RchiOBHm_Chr2g0104361

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000039
Physical Location & Seq
Forward (+)
15727779 .. 15731385
3607 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ47863

Sequence Viewer

Length: 2502 bp
ATGGCCAATCCAGTGGATAAACTTTTCGATGAGCTGTACACAGTCTTTAAAGACCTCATCAAGAAGAATATTGAATTTAAAGGGCAACTTGAAGGCATCGAAGCCAAGCTAGAAGCTCTAAAGCCGGTGATCCCAGACATGGTGCAGAGTAATGAGGTATTAGATCGTTCAAGGAAGGAACTAAAAGGCTTAACAGGAGTTATGGAGTCTGGCATTAAGCTCATTGGCAAGTGCTCAAAAGTTCGGGAGTGGAAGGTCTTCAAGAAGTACAAATACGCCAACAAACTTATTGAATGGGAGAAAAGTCTAGAAAGTGAGTTAGTTATACTGAACGTGCTGACCGGCAGGGATGTGAAGGACATTGCAAGAGATGTGAAGGACATTGCAAGAGATGTGAAGGACATTGCAAGAGAGGAGAAGGAGAATGCAGGGGTTGCAAAGGAGACTGCAGATAGGTTGAAGATTATAGAGGAGAAATTGGACCGTATTGTGATACAAAATCAACCTGGGAAACCTAAAGCCTGGATGAGTACTGTAGTACCTGAACTTCCACGTTTTACAGTGGGATTGGACAAGCATTTGAAGGAATTAAAGATGAAGCTTCTTAAGGACGGGGGGTCAATGCTTGTAATAACAGCTCCTGGAGGGTCTGGCAAAACCACTTTGGCTAAAATGTTTTATCATGATCATGAAGTCAAAGATAAATTCACCAAGAACATCTTTGTTACTGTTTCAACAAAGTCCAACTATATTGTTGTCCAAGAACTATGTCAGAAGTTGGGATCCCAGGAATCTACATTCCCTAATGAAGATGGTGCATTCAGCTGGCTGAAAGAATTTCTGAAGGAAACCTCACAAATTCCTTTGTTTCTTATCCTGGATGATGTTTGGACTGAATCAGAATCCCTTCTTGAGAAGTTTAATGAACTGAGTATACAATCAAATTTCAGTATCTTGGTGACATCAAGATCTTCATTTCCAAGTTTTGGTTCTCAATATCCTTTAAATGCATTGAATCACAAAGATGCGATGACTCTTTTTCGGCATTCAGCAATCCGGGGAGATAAGGGATATAATGTTCCAGAAAATCTTCAGGACAAGATAGTTAGGCGCTGTAAGGGATGTCCACTTGCCATCAAAGTGGTCGGAAAATCTCTTTGTGGAAAATTTACAGAATCATGGCTCAAAAAATTAAGAGAATGGTCCAAAGGTGCTTCTATTTTTGATACCGAGACTGAAGTGTTTCTTTGCCTCAAAAGTAGCTTAGACGCCTTGGATGAAAAAGATCAAACTATCAAGGAATGCTTTTTGGACCTGGGTGCATTTCCTGAAGATCAAAGAATCCCAGCTGTTGCTCTGATTGATATGTGGGCAGAGTTATACGGCCTAGATGAAGATTTTCTTTCCATTCAATACCTCCAGGAGCTTTGCAGCCGAAGCCTGGCTAATCTTGTAGTCACAAGGAAGGAAAAGATGGAGGCGGATGGATACTACAGTGAACACTTTGTCTCCCAGCATGATGTGCTCAGGGATCTAGCTATCTTCCAAGCGAAATTAGATCCAAATAAAACGAGACTGATTTTACACAAATGTGGAGACAAAGTACCCAAGAAGTTGACAGAACAGAAGCATCAATCCTTTCAGACTCGCATATTATCCATCTCCTCTGATGGAGTCTTCTCAACAGATATGCACAAGATTCAACTTTCAGATGTTGAGGTTCTAGTTTTGAATTTTCAGTCAGAGAACCATGCTTTTCCCAAATTTGTGGAGGACATGAAAAACTTGAAGGTCCTAATAGTCACAAATAATGGTTCCTTGCCAGCTGAATTGAGTAACTTTGAACTACTGAAATCCTTGCCAAATCTGAAGAGAATCAGACTGGAGCGAATTTCAATTCCTTCCATAACCAAGAATTGCATACAGTTGAAGAGTGTAAAGAAGATCTCTTTATTCATGTGTAGCATCGGTCAAGCTTTCAGCAACTCTTCCTTCCAAATTTCATATGCATTTCCAAATATAGAGGAATTGAACATTGATTATTGCAGTGATTTGGTGGAATTGCCTGGTGATCTCAGTGATCTGATTGAGCTAAGGAAGCTCAGTATCACCCACTGTCATAAGCTATCTACCTTGCCTGAAGAGATAGGAAAGTTGATTAAATTGGTAGTGTTGAGGCTAAAGTCTTGTACGGACTTGGTAACATTTCCAGCCTCAATTAAGAACCTCAAAATGTTGCAGTTTCTCGACATATCTGATTGCTTCAGCGTCAAGGAGTTGCCTGACGACATTGGTGAAATCAGCACTTTAGAAAAGATCAATATGAGACATTGCTCTAGGCTGAAAGATCTACCTCCATCAGTTTCTGATCTCGAGCAACTAAATGAAGTGATATGTGATGAGGACACAAAAGAGTTATGGGAGATTTTCGGGCTCAAAATAGACATAAGGGTCCTCAAAGACGAATTCGACCTAGATTGGCTCTCAAAGTTCCAATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

833

Amino Acids

94.94

Weight (kDa)

6.17

Isoelectric Point (pI)

38.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 9 - 132 8.5e-21 Arabidopsis broad-spectrum mildew resistance protein RPW8
NB-ARC PF00931 191 - 350 2.3e-15 NB-ARC domain
AAA_22 PF13401 205 - 319 6.6e-08 AAA domain
LRR_14 PF23598 549 - 714 1.8e-06 Leucine-rich repeat region
LRR_14 PF23598 691 - 752 1.6e-06 Leucine-rich repeat region
LRR_14 PF23598 715 - 820 3.5e-10 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000039)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66890 AT5G66900 AT5G66900 AT5G66900 AT5G66910
fragaria_vesca FvH4_1g15090 FvH4_1g15120 FvH4_1g15130 FvH4_1g15140 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15170 FvH4_1g15171 FvH4_1g15171 FvH4_1g15200 FvH4_1g15200 FvH4_1g15200 FvH4_1g15210 FvH4_1g15210 FvH4_1g15210 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15230 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15500 FvH4_1g15500 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_2g19760 FvH4_3g12820 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_6g18970 FvH4_6g42370 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g50100 FvH4_6g50100 FvH4_6g50110 FvH4_6g50130 FvH4_6g50140 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50330
malus_domestica MD00G1053700.v1.1 MD02G1164300.v1.1 MD02G1164400.v1.1 MD02G1164700.v1.1 MD02G1164800.v1.1 MD02G1165300.v1.1 MD02G1165400.v1.1 MD02G1165500.v1.1 MD02G1165600.v1.1 MD02G1165900.v1.1 MD02G1166000.v1.1 MD02G1166100.v1.1 MD02G1166300.v1.1 MD08G1084900.v1.1 MD15G1263100.v1.1 MD15G1263300.v1.1 MD15G1276900.v1.1 MD15G1277200.v1.1 MD15G1277400.v1.1 MD15G1277700.v1.1 MD15G1277800.v1.1 MD15G1278000.v1.1 MD15G1278100.v1.1 MD15G1278400.v1.1 MD15G1278600.v1.1
prunus_persica Prupe.7G138500_v2.0.a1 Prupe.7G138500_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138700_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138900_v2.0.a1 Prupe.7G139100_v2.0.a1 Prupe.7G139200_v2.0.a1 Prupe.7G139500_v2.0.a1 Prupe.7G139600_v2.0.a1 Prupe.7G139700_v2.0.a1 Prupe.7G139800_v2.0.a1
pyrus_communis pycom02g13150 pycom02g13160 pycom02g13190 pycom02g13200 pycom02g13220 pycom02g13230 pycom02g13250 pycom02g13260 pycom02g13270 pycom02g13300 pycom02g13310 pycom02g13330 pycom15g23980 pycom15g23990 pycom15g24030 pycom15g24040 pycom15g24050 pycom15g24070 pycom15g24090 pycom15g24160
rosa_chinensis RchiOBHm_Chr1g0326431 RchiOBHm_Chr1g0326451 RchiOBHm_Chr2g0104121 RchiOBHm_Chr2g0104151 RchiOBHm_Chr2g0104171 RchiOBHm_Chr2g0104181 RchiOBHm_Chr2g0104191 RchiOBHm_Chr2g0104201 RchiOBHm_Chr2g0104211 RchiOBHm_Chr2g0104241 RchiOBHm_Chr2g0104261 RchiOBHm_Chr2g0104281 RchiOBHm_Chr2g0104311 RchiOBHm_Chr2g0104331 RchiOBHm_Chr2g0104361 RchiOBHm_Chr2g0104381 RchiOBHm_Chr2g0104391 RchiOBHm_Chr2g0104431 RchiOBHm_Chr2g0104441 RchiOBHm_Chr2g0104541 RchiOBHm_Chr2g0104611 RchiOBHm_Chr2g0104741 RchiOBHm_Chr2g0104761 RchiOBHm_Chr2g0158291 RchiOBHm_Chr2g0170371 RchiOBHm_Chr2g0170631 RchiOBHm_Chr3g0473971 RchiOBHm_Chr3g0480491 RchiOBHm_Chr3g0480541 RchiOBHm_Chr3g0480561 RchiOBHm_Chr3g0485281 RchiOBHm_Chr3g0485291 RchiOBHm_Chr4g0411541 RchiOBHm_Chr6g0285471
rosa_laevigata RLG00000008347 RLG00000012637 RLG00000016961 RLG00000017293 RLG00000017305 RLG00000017308 RLG00000017312 RLG00000017314 RLG00000017317 RLG00000017318 RLG00000017319 RLG00000017321 RLG00000017322 RLG00000017323 RLG00000017324 RLG00000017325 RLG00000017327 RLG00000017328 RLG00000017329 RLG00000017330 RLG00000017334 RLG00000020833 RLG00000021073 RLG00000021950 RLG00000021971 RLG00000023154 RLG00000023157 RLG00000023467 RLG00000023468 RLG00000023525 RLG00000023963 RLG00000033265 RLG00000033267 RLG00000033268
rosa_multiflora Rmu_co8053082.1_g000001 Rmu_co8210358.1_g000001 Rmu_co8331661.1_g000001 Rmu_co8477427.1_g000002 Rmu_co8483217.1_g000001 Rmu_sc0000284.1_g000004 Rmu_sc0000676.1_g000017 Rmu_sc0000676.1_g000019 Rmu_sc0000676.1_g000021 Rmu_sc0002205.1_g000038 Rmu_sc0002205.1_g000041 Rmu_sc0002316.1_g000010 Rmu_sc0002316.1_g000012 Rmu_sc0002316.1_g000040 Rmu_sc0002316.1_g000071 Rmu_sc0002316.1_g000072 Rmu_sc0002316.1_g000095 Rmu_sc0002316.1_g000102 Rmu_sc0002414.1_g000018 Rmu_sc0002576.1_g000002 Rmu_sc0002576.1_g000003 Rmu_sc0002687.1_g000010 Rmu_sc0002687.1_g000017 Rmu_sc0003660.1_g000001 Rmu_sc0003787.1_g000014 Rmu_sc0003787.1_g000020 Rmu_sc0003787.1_g000024 Rmu_sc0003787.1_g000029 Rmu_sc0004059.1_g000026 Rmu_sc0005137.1_g000021 Rmu_sc0006550.1_g000001 Rmu_sc0006561.1_g000025 Rmu_sc0006561.1_g000033 Rmu_sc0007704.1_g000005 Rmu_sc0008904.1_g000005 Rmu_sc0010270.1_g000002 Rmu_sc0010307.1_g000001 Rmu_sc0011601.1_g000013 Rmu_sc0012694.1_g000005 Rmu_sc0013101.1_g000008 Rmu_sc0019029.1_g000001 Rmu_sc0019728.1_g000001 Rmu_sc0025829.1_g000001 Rmu_sc0025829.1_g000002 Rmu_ssc0000257.1_g000007 Rmu_ssc0000257.1_g000008
rosa_roxburghii Rroxscaffold_1G00021710 Rroxscaffold_1G00049350 Rroxscaffold_1G00049360 Rroxscaffold_2G00081160 Rroxscaffold_2G00081360 Rroxscaffold_2G00081390 Rroxscaffold_2G00091540 Rroxscaffold_2G00138790 Rroxscaffold_2G00138800 Rroxscaffold_2G00138920 Rroxscaffold_2G00139000 Rroxscaffold_2G00139010 Rroxscaffold_2G00139020 Rroxscaffold_2G00139030 Rroxscaffold_2G00139040 Rroxscaffold_2G00139050 Rroxscaffold_2G00139060 Rroxscaffold_2G00139070 Rroxscaffold_2G00139090 Rroxscaffold_2G00139100 Rroxscaffold_3G00257470 Rroxscaffold_4G00318040 Rroxscaffold_4G00323740 Rroxscaffold_4G00323750 Rroxscaffold_5G00356140 Rroxscaffold_6G00401280 Rroxscaffold_6G00401290 Rroxscaffold_7G00183120
rosa_rugosa Rorug01G0060500 Rorug01G0060900 Rorug02G0119500 Rorug02G0119600 Rorug02G0119700 Rorug02G0119700 Rorug02G0119700 Rorug02G0119800.1 Rorug02G0119900.1 Rorug02G0120000 Rorug02G0120100 Rorug02G0120200 Rorug02G0120300 Rorug02G0120400 Rorug02G0120500 Rorug02G0120700 Rorug02G0120700 Rorug02G0122300 Rorug02G0122300 Rorug02G0122400.1 Rorug02G0466700 Rorug02G0466700 Rorug02G0466800 Rorug02G0548600 Rorug02G0549900 Rorug02G0550000 Rorug03G0184200 Rorug04G0107900 Rorug05G0121500 Rorug06G0174800
rosa_samantha Rh1AG198400 Rh1CG074700 Rh1CG075000 Rh1CG183700 Rh2AG169700 Rh2AG169800 Rh2AG169900 Rh2AG170000 Rh2AG170300 Rh2AG170400 Rh2AG171000 Rh2AG171100 Rh2AG171600 Rh2AG172400 Rh2AG172500 Rh2AG532900 Rh2AG620000 Rh2AG622500 Rh2AG622800 Rh2AG623000 Rh2BG176200 Rh2BG176300 Rh2BG176400 Rh2BG176500 Rh2BG176600 Rh2BG176900 Rh2BG177000 Rh2BG177100 Rh2BG177200 Rh2BG177300 Rh2BG177600 Rh2BG177700 Rh2BG177900 Rh2BG178000 Rh2BG178200 Rh2BG178300 Rh2BG178500 Rh2BG179000 Rh2BG180000 Rh2BG180100 Rh2BG259200 Rh2BG546700 Rh2BG630800 Rh2BG633500 Rh2CG176500 Rh2CG177500 Rh2CG177700 Rh2DG174400 Rh2DG174600 Rh2DG174900 Rh2DG175100 Rh2DG175200 Rh2DG175300 Rh2DG175600 Rh2DG175700 Rh2DG175800 Rh2DG175900 Rh2DG176000 Rh2DG176300 Rh2DG176400 Rh2DG176500 Rh2DG176600 Rh2DG176900 Rh2DG177100 Rh2DG177200 Rh2DG177800 Rh2DG177900 Rh2DG178700 Rh2DG178800 Rh2DG555700 Rh2DG643700 Rh2DG646300 Rh3AG235600 Rh3AG235900 Rh3AG236200 Rh3AG266700 Rh3AG266900 Rh3BG216200 Rh3BG269100 Rh3CG212700 Rh3CG265800 Rh4AG168900 Rh4BG165500 Rh4CG180300 Rh5BG261500 Rh6AG286300 Rh6BG287500 Rh6CG288600 Rh7AG022600
rosa_wichuraiana Rw0G008240 Rw0G008250 Rw0G013190 Rw0G013200 Rw2G013230 Rw2G013240 Rw2G013270 Rw2G013280 Rw2G013290 Rw2G013300 Rw2G013330 Rw2G013340 Rw2G013350 Rw2G013360 Rw2G013370 Rw2G013380 Rw2G013410 Rw2G013570 Rw2G044120 Rw2G051370 Rw2G051590 Rw3G017160 Rw3G021150 Rw3G021160 Rw3G023730 Rw3G023740 Rw4G014030 Rw6G024590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2450
AccB7I CCANNNNNTGG 1 cut(s) 986
AccI GTMKAC 1 cut(s) 934
AciI CCGC 1 cut(s) 1481
AclWI GGATC 5 cut(s) 124, 777, 790, 1539, 1553
AcoI YGGCCR 1 cut(s) 3
AcuI CTGAAG 7 cut(s) 863, 1076, 1257, 1350, 1889, 2160, 2248
AcyI GRCGYC 1 cut(s) 1269
AfaI GTAC 6 cut(s) 38, 269, 532, 540, 1605, 2191
AfiI CCNNNNNNNGG 3 cut(s) 139, 986, 1441
AflII CTTAAG 1 cut(s) 605
AhdI GACNNNNNGTC 1 cut(s) 616
AjnI CCWGG 9 cut(s) 505, 521, 640, 786, 876, 1314, 1419, 1440, 2065
AjuI GAANNNNNNNTTGG 2 cut(s) 973, 1005
AleI CACNNNNGTG 1 cut(s) 1590
AloI GAACNNNNNNTCC 2 cut(s) 1062, 1094
Alw21I GWGCWC 2 cut(s) 236, 1527
Alw26I GTCTC 6 cut(s) 437, 1226, 1513, 1567, 1590, 2320
AlwI GGATC 5 cut(s) 124, 777, 790, 1539, 1553
AlwNI CAGNNNCTG 2 cut(s) 641, 2366
Ama87I CYCGRG 1 cut(s) 2372
AoxI GGCC 2 cut(s) 3, 1384
ApeKI GCWGC 1 cut(s) 1431
Asp700I GAANNNNTTC 5 cut(s) 257, 906, 1089, 1242, 1398
AspLEI GCGC 1 cut(s) 1113
AspS9I GGNCC 5 cut(s) 481, 1203, 1312, 1792, 2452
AsuC2I CCSGG 1 cut(s) 1058
AsuHPI GGTGA 6 cut(s) 139, 700, 970, 2081, 2101, 2306
AvaI CYCGRG 1 cut(s) 2372
AvaII GGWCC 5 cut(s) 481, 1203, 1312, 1792, 2452
BaeI ACNNNNGTAYC 4 cut(s) 522, 555, 1480, 1513
BalI TGGCCA 1 cut(s) 5
BamHI GGATCC 1 cut(s) 782
BanII GRGCYC 1 cut(s) 2436
BarI GAAGNNNNNNTAC 4 cut(s) 257, 289, 531, 563
BbsI GAAGAC 2 cut(s) 250, 1669
Bbv12I GWGCWC 2 cut(s) 236, 1527
BbvI GCAGC 1 cut(s) 1443
BccI CCATC 7 cut(s) 806, 1142, 1468, 1478, 1664, 1667, 2365
BceAI ACGGC 1 cut(s) 1399
BciT130I CCWGG 9 cut(s) 507, 523, 642, 788, 878, 1316, 1421, 1442, 2067
BciVI GTATCC 1 cut(s) 1481
BclI TGATCA 1 cut(s) 685
BcnI CCSGG 1 cut(s) 1058
BcoDI GTCTC 6 cut(s) 437, 1226, 1513, 1567, 1590, 2320
BfaI CTAG 7 cut(s) 110, 308, 1388, 1535, 1724, 2337, 2474
BfmI CTRYAG 3 cut(s) 447, 534, 1492
BfoI RGCGCY 1 cut(s) 1114
BfrI CTTAAG 1 cut(s) 605
BfuI GTATCC 1 cut(s) 1481
BglII AGATCT 3 cut(s) 968, 1944, 2347
BisI GCNGC 1 cut(s) 1432
BlsI GCNGC 1 cut(s) 1433
BmcAI AGTACT 1 cut(s) 532
Bme18I GGWCC 5 cut(s) 481, 1203, 1312, 1792, 2452
BmeRI GACNNNNNGTC 1 cut(s) 616
BmeT110I CYCGRG 1 cut(s) 2372
BmgT120I GGNCC 5 cut(s) 481, 1203, 1312, 1792, 2452
BmiI GGNNCC 3 cut(s) 784, 1816, 2453
BmsI GCATC 4 cut(s) 105, 1015, 1639, 1974
BpiI GAAGAC 2 cut(s) 250, 1669
BpmI CTGGAG 3 cut(s) 663, 1403, 1904
Bpu10I CCTNAGC 2 cut(s) 1526, 2093
BpuEI CTTGAG 1 cut(s) 932
BpuMI CCSGG 1 cut(s) 1058
BsaHI GRCGYC 1 cut(s) 1269
BsaJI CCNNGG 5 cut(s) 506, 786, 1057, 1272, 1315
BsaXI ACNNNNNCTCC 4 cut(s) 1493, 1523, 1587, 1617
Bsc4I CCNNNNNNNGG 3 cut(s) 139, 986, 1441
Bse118I RCCGGY 2 cut(s) 124, 341
Bse1I ACTGG 2 cut(s) 11, 1887
Bse3DI GCAATG 4 cut(s) 360, 381, 402, 2329
BseBI CCWGG 9 cut(s) 507, 523, 642, 788, 878, 1316, 1421, 1442, 2067
BseDI CCNNGG 5 cut(s) 506, 786, 1057, 1272, 1315
BseGI GGATG 6 cut(s) 355, 531, 886, 1127, 1282, 1489
BseLI CCNNNNNNNGG 3 cut(s) 139, 986, 1441
BseMI GCAATG 4 cut(s) 360, 381, 402, 2329
BseMII CTCAG 4 cut(s) 920, 1540, 2089, 2116
BseNI ACTGG 2 cut(s) 11, 1887
BseRI GAGGAG 3 cut(s) 428, 485, 1654
BseXI GCAGC 1 cut(s) 1443
BseYI CCCAGC 2 cut(s) 1345, 1512
BsgI GTGCAG 1 cut(s) 164
BshFI GGCC 2 cut(s) 5, 1386
BsiHKAI GWGCWC 2 cut(s) 236, 1527
BsiHKCI CYCGRG 1 cut(s) 2372
BsiSI CCGG 3 cut(s) 125, 342, 1057
BslI CCNNNNNNNGG 3 cut(s) 139, 986, 1441
BsmAI GTCTC 6 cut(s) 437, 1226, 1513, 1567, 1590, 2320
BsmI GAATGC 4 cut(s) 430, 818, 1045, 1307
BsnI GGCC 2 cut(s) 5, 1386
BsoBI CYCGRG 1 cut(s) 2372
Bsp1286I GDGCHC 3 cut(s) 236, 1527, 2436
Bsp1407I TGTACA 1 cut(s) 36
BspACI CCGC 1 cut(s) 1481
BspANI GGCC 2 cut(s) 5, 1386
BspCNI CTCAG 4 cut(s) 921, 1539, 2088, 2115
BspHI TCATGA 2 cut(s) 682, 688
BspLI GGNNCC 3 cut(s) 784, 1816, 2453
BspMAI CTGCAG 1 cut(s) 451
BspPI GGATC 5 cut(s) 124, 777, 790, 1539, 1553
BspTI CTTAAG 1 cut(s) 605
BsrDI GCAATG 4 cut(s) 360, 381, 402, 2329
BsrFI RCCGGY 2 cut(s) 124, 341
BsrGI TGTACA 1 cut(s) 36
BsrI ACTGG 2 cut(s) 11, 1887
BssAI RCCGGY 2 cut(s) 124, 341
BssECI CCNNGG 5 cut(s) 506, 786, 1057, 1272, 1315
BssNAI GTATAC 1 cut(s) 935
BssNI GRCGYC 1 cut(s) 1269
BssT1I CCWWGG 1 cut(s) 1272
Bst1107I GTATAC 1 cut(s) 935
Bst2UI CCWGG 9 cut(s) 507, 523, 642, 788, 878, 1316, 1421, 1442, 2067
Bst4CI ACNGT 8 cut(s) 43, 485, 535, 562, 730, 1496, 1926, 2117
Bst6I CTCTTC 4 cut(s) 1865, 1925, 1993, 2136
BstACI GRCGYC 1 cut(s) 1269
BstAFI CTTAAG 1 cut(s) 605
BstAPI GCANNNNNTGC 2 cut(s) 434, 1522
BstAUI TGTACA 1 cut(s) 36
BstC8I GCNNGC 2 cut(s) 827, 1824
BstDEI CTNAG 6 cut(s) 929, 1264, 1526, 2075, 2093, 2102
BstF5I GGATG 6 cut(s) 355, 531, 886, 1127, 1282, 1489
BstH2I RGCGCY 1 cut(s) 1114
BstHHI GCGC 1 cut(s) 1113
BstMAI GTCTC 6 cut(s) 437, 1226, 1513, 1567, 1590, 2320
BstMWI GCNNNNNNNGC 4 cut(s) 434, 1437, 1522, 2098
BstNI CCWGG 9 cut(s) 507, 523, 642, 788, 878, 1316, 1421, 1442, 2067
BstSFI CTRYAG 3 cut(s) 447, 534, 1492
BstV1I GCAGC 1 cut(s) 1443
BstV2I GAAGAC 2 cut(s) 250, 1669
BstX2I RGATCY 6 cut(s) 782, 968, 1531, 1558, 1944, 2347
BstXI CCANNNNNNTGG 3 cut(s) 13, 1141, 1768
BstYI RGATCY 6 cut(s) 782, 968, 1531, 1558, 1944, 2347
BstZ17I GTATAC 1 cut(s) 935
BsuI GTATCC 1 cut(s) 1481
BsuRI GGCC 2 cut(s) 5, 1386
BtgZI GCGATG 1 cut(s) 1043
BtsCI GGATG 6 cut(s) 355, 531, 886, 1127, 1282, 1489
BtsI GCAGTG 1 cut(s) 2053
BtsIMutI CAGTG 6 cut(s) 18, 567, 1501, 2053, 2083, 2113
Cac8I GCNNGC 2 cut(s) 827, 1824
CaiI CAGNNNCTG 2 cut(s) 641, 2366
CciI TCATGA 2 cut(s) 682, 688
CfoI GCGC 1 cut(s) 1113
Cfr10I RCCGGY 2 cut(s) 124, 341
Cfr13I GGNCC 5 cut(s) 481, 1203, 1312, 1792, 2452
CseI GACGC 2 cut(s) 1277, 2257
Csp6I GTAC 6 cut(s) 37, 268, 531, 539, 1604, 2190
CviAII CATG 8 cut(s) 139, 683, 689, 1179, 1517, 1751, 1777, 1957
CviQI GTAC 6 cut(s) 37, 268, 531, 539, 1604, 2190
DdeI CTNAG 6 cut(s) 929, 1264, 1526, 2075, 2093, 2102
DraI TTTAAA 3 cut(s) 49, 79, 1005
DrdI GACNNNNNNGTC 1 cut(s) 2450
DriI GACNNNNNGTC 1 cut(s) 616
DseDI GACNNNNNNGTC 1 cut(s) 2450
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 4 cut(s) 1865, 1925, 1993, 2136
Eam1105I GACNNNNNGTC 1 cut(s) 616
EarI CTCTTC 4 cut(s) 1865, 1925, 1993, 2136
EciI GGCGGA 1 cut(s) 1496
Eco130I CCWWGG 1 cut(s) 1272
Eco24I GRGCYC 1 cut(s) 2436
Eco47I GGWCC 5 cut(s) 481, 1203, 1312, 1792, 2452
Eco57I CTGAAG 7 cut(s) 863, 1076, 1257, 1350, 1889, 2160, 2248
Eco88I CYCGRG 1 cut(s) 2372
EcoO109I RGGNCCY 2 cut(s) 1792, 2452
EcoRI GAATTC 1 cut(s) 2465
EcoRII CCWGG 9 cut(s) 505, 521, 640, 786, 876, 1314, 1419, 1440, 2065
EcoT14I CCWWGG 1 cut(s) 1272
EcoT22I ATGCAT 2 cut(s) 1012, 2011
EcoT38I GRGCYC 1 cut(s) 2436
ErhI CCWWGG 1 cut(s) 1272
FaeI CATG 8 cut(s) 142, 686, 692, 1182, 1520, 1754, 1780, 1960
FatI CATG 8 cut(s) 138, 682, 688, 1178, 1516, 1750, 1776, 1956
FauNDI CATATG 1 cut(s) 2005
FbaI TGATCA 1 cut(s) 685
FblI GTMKAC 1 cut(s) 934
Fnu4HI GCNGC 1 cut(s) 1432
FokI GGATG 6 cut(s) 362, 538, 893, 1134, 1289, 1496
FriOI GRGCYC 1 cut(s) 2436
Fsp4HI GCNGC 1 cut(s) 1432
FspBI CTAG 7 cut(s) 110, 308, 1388, 1535, 1724, 2337, 2474
GlaI GCGC 1 cut(s) 1112
GluI GCNGC 1 cut(s) 1432
GsaI CCCAGC 2 cut(s) 1349, 1516
GsuI CTGGAG 3 cut(s) 663, 1403, 1904
HaeII RGCGCY 1 cut(s) 1114
HaeIII GGCC 2 cut(s) 5, 1386
HapII CCGG 3 cut(s) 125, 342, 1057
HgaI GACGC 2 cut(s) 1277, 2257
HhaI GCGC 1 cut(s) 1113
Hin1I GRCGYC 1 cut(s) 1269
Hin1II CATG 8 cut(s) 142, 686, 692, 1182, 1520, 1754, 1780, 1960
Hin6I GCGC 1 cut(s) 1111
HinP1I GCGC 1 cut(s) 1111
HincII GTYRAC 1 cut(s) 1617
HindII GTYRAC 1 cut(s) 1617
HindIII AAGCTT 2 cut(s) 599, 1974
HpaII CCGG 3 cut(s) 125, 342, 1057
HphI GGTGA 6 cut(s) 139, 700, 970, 2081, 2101, 2306
Hpy166II GTNNAC 5 cut(s) 39, 935, 1127, 1499, 1617
Hpy8I GTNNAC 5 cut(s) 39, 935, 1127, 1499, 1617
HpyCH4III ACNGT 8 cut(s) 43, 485, 535, 562, 730, 1496, 1926, 2117
HpyCH4IV ACGT 2 cut(s) 333, 553
HpyF10VI GCNNNNNNNGC 4 cut(s) 434, 1437, 1522, 2098
HpyF3I CTNAG 6 cut(s) 929, 1264, 1526, 2075, 2093, 2102
HpySE526I ACGT 2 cut(s) 333, 553
Hsp92I GRCGYC 1 cut(s) 1269
Hsp92II CATG 8 cut(s) 142, 686, 692, 1182, 1520, 1754, 1780, 1960
HspAI GCGC 1 cut(s) 1111
Ksp22I TGATCA 1 cut(s) 685
LmnI GCTCC 3 cut(s) 643, 1423, 1885
Lsp1109I GCAGC 1 cut(s) 1443
LweI GCATC 4 cut(s) 105, 1015, 1639, 1974
MaeI CTAG 7 cut(s) 110, 308, 1388, 1535, 1724, 2337, 2474
MaeII ACGT 2 cut(s) 333, 553
MaeIII GTNAC 6 cut(s) 724, 958, 1456, 1801, 1835, 2200
MflI RGATCY 6 cut(s) 782, 968, 1531, 1558, 1944, 2347
MhlI GDGCHC 3 cut(s) 236, 1527, 2436
MlsI TGGCCA 1 cut(s) 5
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 4 cut(s) 215, 1027, 1639, 1683
MmeI TCCRAC 2 cut(s) 768, 1126
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 2 cut(s) 1012, 2011
MroXI GAANNNNTTC 5 cut(s) 257, 906, 1089, 1242, 1398
MscI TGGCCA 1 cut(s) 5
MslI CAYNNNNRTG 4 cut(s) 687, 1023, 1139, 1590
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 3 cut(s) 825, 1349, 1826
MspCI CTTAAG 1 cut(s) 605
MspI CCGG 3 cut(s) 125, 342, 1057
Mva1269I GAATGC 4 cut(s) 430, 818, 1045, 1307
MvaI CCWGG 9 cut(s) 507, 523, 642, 788, 878, 1316, 1421, 1442, 2067
MwoI GCNNNNNNNGC 4 cut(s) 434, 1437, 1522, 2098
NciI CCSGG 1 cut(s) 1058
NdeI CATATG 1 cut(s) 2005
NlaIII CATG 8 cut(s) 142, 686, 692, 1182, 1520, 1754, 1780, 1960
NlaIV GGNNCC 3 cut(s) 784, 1816, 2453
NmuCI GTSAC 3 cut(s) 958, 1456, 1801
NsiI ATGCAT 2 cut(s) 1012, 2011
OliI CACNNNNGTG 1 cut(s) 1590
PaeR7I CTCGAG 1 cut(s) 2372
PagI TCATGA 2 cut(s) 682, 688
PctI GAATGC 4 cut(s) 430, 818, 1045, 1307
PdmI GAANNNNTTC 5 cut(s) 257, 906, 1089, 1242, 1398
PfeI GAWTC 8 cut(s) 791, 896, 902, 1015, 1175, 1341, 1699, 1875
PflMI CCANNNNNTGG 1 cut(s) 986
PfoI TCCNGGA 3 cut(s) 640, 876, 1419
PkrI GCNGC 1 cut(s) 1433
PleI GAGTC 4 cut(s) 214, 1027, 1639, 1682
PpsI GAGTC 4 cut(s) 214, 1027, 1639, 1682
PpuMI RGGWCCY 2 cut(s) 1792, 2452
Psp5II RGGWCCY 2 cut(s) 1792, 2452
Psp6I CCWGG 9 cut(s) 505, 521, 640, 786, 876, 1314, 1419, 1440, 2065
PspFI CCCAGC 2 cut(s) 1345, 1512
PspGI CCWGG 9 cut(s) 505, 521, 640, 786, 876, 1314, 1419, 1440, 2065
PspN4I GGNNCC 3 cut(s) 784, 1816, 2453
PspPI GGNCC 5 cut(s) 481, 1203, 1312, 1792, 2452
PspPPI RGGWCCY 2 cut(s) 1792, 2452
PsrI GAACNNNNNNTAC 2 cut(s) 918, 950
PstI CTGCAG 1 cut(s) 451
PstNI CAGNNNCTG 2 cut(s) 641, 2366
PsuI RGATCY 6 cut(s) 782, 968, 1531, 1558, 1944, 2347
PvuII CAGCTG 3 cut(s) 825, 1349, 1826
RsaI GTAC 6 cut(s) 38, 269, 532, 540, 1605, 2191
RsaNI GTAC 6 cut(s) 37, 268, 531, 539, 1604, 2190
RseI CAYNNNNRTG 4 cut(s) 687, 1023, 1139, 1590
SatI GCNGC 1 cut(s) 1432
Sau96I GGNCC 5 cut(s) 481, 1203, 1312, 1792, 2452
ScaI AGTACT 1 cut(s) 532
SchI GAGTC 4 cut(s) 215, 1027, 1639, 1683
SduI GDGCHC 3 cut(s) 236, 1527, 2436
SfaNI GCATC 4 cut(s) 105, 1015, 1639, 1974
SfcI CTRYAG 3 cut(s) 447, 534, 1492
Sfr274I CTCGAG 1 cut(s) 2372
SinI GGWCC 5 cut(s) 481, 1203, 1312, 1792, 2452
SlaI CTCGAG 1 cut(s) 2372
SmiMI CAYNNNNRTG 4 cut(s) 687, 1023, 1139, 1590
SmlI CTYRAG 3 cut(s) 605, 911, 2372
SmoI CTYRAG 3 cut(s) 605, 911, 2372
SsiI CCGC 1 cut(s) 1481
SspI AATATT 1 cut(s) 70
SspMI CTAG 7 cut(s) 110, 308, 1388, 1535, 1724, 2337, 2474
StyI CCWWGG 1 cut(s) 1272
TaaI ACNGT 8 cut(s) 43, 485, 535, 562, 730, 1496, 1926, 2117
TaiI ACGT 2 cut(s) 336, 556
TaqI TCGA 5 cut(s) 27, 99, 2247, 2373, 2469
TaqII GACCGA 1 cut(s) 1958
TatI WGTACW 3 cut(s) 36, 267, 530
TfiI GAWTC 8 cut(s) 791, 896, 902, 1015, 1175, 1341, 1699, 1875
TscAI CASTG 6 cut(s) 18, 567, 1501, 2053, 2083, 2120
TseFI GTSAC 3 cut(s) 958, 1456, 1801
TseI GCWGC 1 cut(s) 1431
Tsp45I GTSAC 3 cut(s) 958, 1456, 1801
TspGWI ACGGA 1 cut(s) 2207
TspRI CASTG 6 cut(s) 18, 567, 1501, 2053, 2083, 2120
Van91I CCANNNNNTGG 1 cut(s) 986
Vha464I CTTAAG 1 cut(s) 605
VpaK11BI GGWCC 5 cut(s) 481, 1203, 1312, 1792, 2452
XbaI TCTAGA 1 cut(s) 307
XhoI CTCGAG 1 cut(s) 2372
XmiI GTMKAC 1 cut(s) 934
XmnI GAANNNNTTC 5 cut(s) 257, 906, 1089, 1242, 1398
XspI CTAG 7 cut(s) 110, 308, 1388, 1535, 1724, 2337, 2474
ZrmI AGTACT 1 cut(s) 532
Zsp2I ATGCAT 2 cut(s) 1012, 2011
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.