RchiOBHm_Chr2g0104311

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000039
Physical Location & Seq
Forward (+)
15693723 .. 15697437
3715 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ47858

Sequence Viewer

Length: 2562 bp
ATGGCCAATCCAGCTGATGTTTTCGCCGGGGCTGCTGGAGGATTACTATTCAATGGGCTCTACGATGGCATTAAGGAGGTGGCCACCAAGCATGCGATGTTCAAAGACGTCCTCGAAAGCGTCAAAACCACACTCGAATCTTTGAAGAAAGTGGTTGAAGACATAGCACAACTGAACGAGGATCTGGATCGCCCCAAGGAAGAAATAGATAGTCTCAAAACCCGACTAGAGGAGGGCAAAGAGCTCGTTGCCAAGTGCCTCAAGGTTCGTAAGTGGAGGGTGGACGAGAAGTACAAGTACTCCAAGAAACTTCTTGGATATGATGACAGGCTCAAAAAACAGTTAGAGAGACTCGAAGTTTATGCAATAAGAGATGGGATGGAGACCTTGACAACGGTAAAGAGAACAGAGAAGACTGTGAGCCAAATTGAATGGAGCAGTTCCAGTATAAAGGAGAGAGTTGAGCAAATTGAGACCAACACTGCAAATCTACCGATGGCGGTGAGCCGAGTGGAAGCGATGTGTTTGAATATACAGGCTGAGATGAAGCAGACACAAAAGGAAGCTAAACCTGAAGCTTGGTATGCAGTACCTGAACTTCCAACTCTTACAGTTGGATTGGATGGGCCTTTGAACGAACTGAAGATGAAGTTGTTAAACAATGGAGTGTCAATGGTTGTGCTGACCGCTCCCGGAGGATGTGGAAAAACCACTTTGGCAACAAAGTTCTGTCAAGATAAGCAAGTCCAAGAAAAATTCAAGAACAATATTTTCTTTATCACTGTTTCCGAAACGCCAGACTTCTACCTTATTGTACAAAGGCTATACCGAAGCAAGGGTAACCCGGCACCTACTTTTAAAGATGATGAAAATGCACTCAAGTTGCTGCGAAAATTTCTGAAGGAAGAAGGACAGAACCCTTTACTGTTGGTCTTGGATGATGTTTGGTCTGGGTCAGACTCCTTACTTGAGAAGTTTGAATTCAAAATGCAGGATTACAAAGTTTTAGTGACATCAAGATCTGAATTTCGAAGATTTGACTCTTATGCATTGCAGTCATTGGATTTTGACAATGCAATGAAACTCTTTCATCATGCAGCATCCTTGGGAGAAAAAAGTTTGGATATTCCACCAGATATTTCAAGAAAGGTAGTAGAGGGCTGTAAGGGATTTCCACTTGCTATAACAGTGGCTGGAAAATCACTTTGCGGACAGCCTATAGAGATTTGGCAGGAAAGACTCATTAAATGGTCCAAACATTCTTCGATTCTTGATTCCGAGACTGAATTGCTTCTTTGCCTCAAAACCAGTTTAGATGTCTTGAATAAGGAAAGGAGTATGGTCAAAGAATGTTTCTTAGACCTAGGTTCATTTCCTGAAGATCAAAGGATCCCTGTTGCTGCCCTCATTGATATCTGGACAGAGTCTTACAGTTCACTGGATCAAGACATTTTGTGTGTTGCAAACCTCCACCAGATCACCAACCGAAGTCTAGCTAATCTTGTAGTCACAAGGAAGGACAAGCCGCAGGGGGATGGATACTACAGTGAGCATTTTGTTACCCAGCATGATCTGCTTAGAGAGCTGGCTATCTATGAGACAAGACAAGAGGACCCCGGACATAGAGAAAGACTAATTATAAACATATCTGAAGACAATTTTCCCAAGTGGTGGAGCGAACAGAAGTATCCACGCATGAAGGCTCGCCTGTTATCTATTTCAACTGATGGATCGTTTTCAACAAAATGGCACGACATGGAACTACCAGAATCTGAGGTTCTAGTCTTGAATTTTCATACCAAGAACTATCCATTACCCCAATTTGTGGAGAAAATGGTTAAGTTGAAGGTACTGGTAGTCACAAATTATGGTATTGTACCTTCAGAATTAACAAATTTTGAAATACTGTGTACGTCATCAAAGTTGAAGCGAATGAGATTAGAACGCATCTCAATTCCATCGATAGCCAAGCACCCCATGCAGTTGAATAGTCTCCAGAAGATTTCTTTATTCATGTGTAATATTGGTCAAGCTTTCAGCAATGGGTCAATCCAATTTTCAAATGCATGGCCAAATCTAGTGGAAGTGAACATTGACTATTGCAATGATTTGGTGGCATTGCCTGCTGACACCAGTGATTTGATTCATCTAAAGAAGCTTAGCATCACCAACTGTCATAAGCTACTTGCCTTGCCTGAAGATATTGGAAAGCTGGCCAAATTAGAATTGTTGAGGCTAAGGTCTTGTACAGACTTAGCAAAGTTGCCGGGTTCGACTGGGAACCTCAAGAAGCTAAAATCTCTCGATATATCTTACTGCTTCAGCATTAAGGAGTTGCCTGAAGACTTTGGTGAATTGAGCTGCTTAAGAGAGCTTAACATGAGAGACTGCTCAAGATTGCAAGAGCTGCCTCTATCAGTTTCAAATCTGGAGTATTTGAAAGTGATAGGTGATGATGATACACAAAGTTTATGGCAACCCTTCTTACCTATCGAAGGCACACACATAGTGGTGGCCAAGGAGAATATCAACCTAAATTGGCTCCCCAAGTTTCAGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

853

Amino Acids

97.18

Weight (kDa)

6.17

Isoelectric Point (pI)

40.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 5 - 138 3e-25 Arabidopsis broad-spectrum mildew resistance protein RPW8
NB-ARC PF00931 210 - 342 2.2e-15 NB-ARC domain
LRR_14 PF23598 711 - 814 3.1e-11 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000039)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66890 AT5G66900 AT5G66900 AT5G66900 AT5G66910
fragaria_vesca FvH4_1g15090 FvH4_1g15120 FvH4_1g15130 FvH4_1g15140 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15170 FvH4_1g15171 FvH4_1g15171 FvH4_1g15200 FvH4_1g15200 FvH4_1g15200 FvH4_1g15210 FvH4_1g15210 FvH4_1g15210 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15230 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15500 FvH4_1g15500 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_2g19760 FvH4_3g12820 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_6g18970 FvH4_6g42370 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g50100 FvH4_6g50100 FvH4_6g50110 FvH4_6g50130 FvH4_6g50140 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50330
malus_domestica MD00G1053700.v1.1 MD02G1164300.v1.1 MD02G1164400.v1.1 MD02G1164700.v1.1 MD02G1164800.v1.1 MD02G1165300.v1.1 MD02G1165400.v1.1 MD02G1165500.v1.1 MD02G1165600.v1.1 MD02G1165900.v1.1 MD02G1166000.v1.1 MD02G1166100.v1.1 MD02G1166300.v1.1 MD08G1084900.v1.1 MD15G1263100.v1.1 MD15G1263300.v1.1 MD15G1276900.v1.1 MD15G1277200.v1.1 MD15G1277400.v1.1 MD15G1277700.v1.1 MD15G1277800.v1.1 MD15G1278000.v1.1 MD15G1278100.v1.1 MD15G1278400.v1.1 MD15G1278600.v1.1
prunus_persica Prupe.7G138500_v2.0.a1 Prupe.7G138500_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138700_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138900_v2.0.a1 Prupe.7G139100_v2.0.a1 Prupe.7G139200_v2.0.a1 Prupe.7G139500_v2.0.a1 Prupe.7G139600_v2.0.a1 Prupe.7G139700_v2.0.a1 Prupe.7G139800_v2.0.a1
pyrus_communis pycom02g13150 pycom02g13160 pycom02g13190 pycom02g13200 pycom02g13220 pycom02g13230 pycom02g13250 pycom02g13260 pycom02g13270 pycom02g13300 pycom02g13310 pycom02g13330 pycom15g23980 pycom15g23990 pycom15g24030 pycom15g24040 pycom15g24050 pycom15g24070 pycom15g24090 pycom15g24160
rosa_chinensis RchiOBHm_Chr1g0326431 RchiOBHm_Chr1g0326451 RchiOBHm_Chr2g0104121 RchiOBHm_Chr2g0104151 RchiOBHm_Chr2g0104171 RchiOBHm_Chr2g0104181 RchiOBHm_Chr2g0104191 RchiOBHm_Chr2g0104201 RchiOBHm_Chr2g0104211 RchiOBHm_Chr2g0104241 RchiOBHm_Chr2g0104261 RchiOBHm_Chr2g0104281 RchiOBHm_Chr2g0104311 RchiOBHm_Chr2g0104331 RchiOBHm_Chr2g0104361 RchiOBHm_Chr2g0104381 RchiOBHm_Chr2g0104391 RchiOBHm_Chr2g0104431 RchiOBHm_Chr2g0104441 RchiOBHm_Chr2g0104541 RchiOBHm_Chr2g0104611 RchiOBHm_Chr2g0104741 RchiOBHm_Chr2g0104761 RchiOBHm_Chr2g0158291 RchiOBHm_Chr2g0170371 RchiOBHm_Chr2g0170631 RchiOBHm_Chr3g0473971 RchiOBHm_Chr3g0480491 RchiOBHm_Chr3g0480541 RchiOBHm_Chr3g0480561 RchiOBHm_Chr3g0485281 RchiOBHm_Chr3g0485291 RchiOBHm_Chr4g0411541 RchiOBHm_Chr6g0285471
rosa_laevigata RLG00000008347 RLG00000012637 RLG00000016961 RLG00000017293 RLG00000017305 RLG00000017308 RLG00000017312 RLG00000017314 RLG00000017317 RLG00000017318 RLG00000017319 RLG00000017321 RLG00000017322 RLG00000017323 RLG00000017324 RLG00000017325 RLG00000017327 RLG00000017328 RLG00000017329 RLG00000017330 RLG00000017334 RLG00000020833 RLG00000021073 RLG00000021950 RLG00000021971 RLG00000023154 RLG00000023157 RLG00000023467 RLG00000023468 RLG00000023525 RLG00000023963 RLG00000033265 RLG00000033267 RLG00000033268
rosa_multiflora Rmu_co8053082.1_g000001 Rmu_co8210358.1_g000001 Rmu_co8331661.1_g000001 Rmu_co8477427.1_g000002 Rmu_co8483217.1_g000001 Rmu_sc0000284.1_g000004 Rmu_sc0000676.1_g000017 Rmu_sc0000676.1_g000019 Rmu_sc0000676.1_g000021 Rmu_sc0002205.1_g000038 Rmu_sc0002205.1_g000041 Rmu_sc0002316.1_g000010 Rmu_sc0002316.1_g000012 Rmu_sc0002316.1_g000040 Rmu_sc0002316.1_g000071 Rmu_sc0002316.1_g000072 Rmu_sc0002316.1_g000095 Rmu_sc0002316.1_g000102 Rmu_sc0002414.1_g000018 Rmu_sc0002576.1_g000002 Rmu_sc0002576.1_g000003 Rmu_sc0002687.1_g000010 Rmu_sc0002687.1_g000017 Rmu_sc0003660.1_g000001 Rmu_sc0003787.1_g000014 Rmu_sc0003787.1_g000020 Rmu_sc0003787.1_g000024 Rmu_sc0003787.1_g000029 Rmu_sc0004059.1_g000026 Rmu_sc0005137.1_g000021 Rmu_sc0006550.1_g000001 Rmu_sc0006561.1_g000025 Rmu_sc0006561.1_g000033 Rmu_sc0007704.1_g000005 Rmu_sc0008904.1_g000005 Rmu_sc0010270.1_g000002 Rmu_sc0010307.1_g000001 Rmu_sc0011601.1_g000013 Rmu_sc0012694.1_g000005 Rmu_sc0013101.1_g000008 Rmu_sc0019029.1_g000001 Rmu_sc0019728.1_g000001 Rmu_sc0025829.1_g000001 Rmu_sc0025829.1_g000002 Rmu_ssc0000257.1_g000007 Rmu_ssc0000257.1_g000008
rosa_roxburghii Rroxscaffold_1G00021710 Rroxscaffold_1G00049350 Rroxscaffold_1G00049360 Rroxscaffold_2G00081160 Rroxscaffold_2G00081360 Rroxscaffold_2G00081390 Rroxscaffold_2G00091540 Rroxscaffold_2G00138790 Rroxscaffold_2G00138800 Rroxscaffold_2G00138920 Rroxscaffold_2G00139000 Rroxscaffold_2G00139010 Rroxscaffold_2G00139020 Rroxscaffold_2G00139030 Rroxscaffold_2G00139040 Rroxscaffold_2G00139050 Rroxscaffold_2G00139060 Rroxscaffold_2G00139070 Rroxscaffold_2G00139090 Rroxscaffold_2G00139100 Rroxscaffold_3G00257470 Rroxscaffold_4G00318040 Rroxscaffold_4G00323740 Rroxscaffold_4G00323750 Rroxscaffold_5G00356140 Rroxscaffold_6G00401280 Rroxscaffold_6G00401290 Rroxscaffold_7G00183120
rosa_rugosa Rorug01G0060500 Rorug01G0060900 Rorug02G0119500 Rorug02G0119600 Rorug02G0119700 Rorug02G0119700 Rorug02G0119700 Rorug02G0119800.1 Rorug02G0119900.1 Rorug02G0120000 Rorug02G0120100 Rorug02G0120200 Rorug02G0120300 Rorug02G0120400 Rorug02G0120500 Rorug02G0120700 Rorug02G0120700 Rorug02G0122300 Rorug02G0122300 Rorug02G0122400.1 Rorug02G0466700 Rorug02G0466700 Rorug02G0466800 Rorug02G0548600 Rorug02G0549900 Rorug02G0550000 Rorug03G0184200 Rorug04G0107900 Rorug05G0121500 Rorug06G0174800
rosa_samantha Rh1AG198400 Rh1CG074700 Rh1CG075000 Rh1CG183700 Rh2AG169700 Rh2AG169800 Rh2AG169900 Rh2AG170000 Rh2AG170300 Rh2AG170400 Rh2AG171000 Rh2AG171100 Rh2AG171600 Rh2AG172400 Rh2AG172500 Rh2AG532900 Rh2AG620000 Rh2AG622500 Rh2AG622800 Rh2AG623000 Rh2BG176200 Rh2BG176300 Rh2BG176400 Rh2BG176500 Rh2BG176600 Rh2BG176900 Rh2BG177000 Rh2BG177100 Rh2BG177200 Rh2BG177300 Rh2BG177600 Rh2BG177700 Rh2BG177900 Rh2BG178000 Rh2BG178200 Rh2BG178300 Rh2BG178500 Rh2BG179000 Rh2BG180000 Rh2BG180100 Rh2BG259200 Rh2BG546700 Rh2BG630800 Rh2BG633500 Rh2CG176500 Rh2CG177500 Rh2CG177700 Rh2DG174400 Rh2DG174600 Rh2DG174900 Rh2DG175100 Rh2DG175200 Rh2DG175300 Rh2DG175600 Rh2DG175700 Rh2DG175800 Rh2DG175900 Rh2DG176000 Rh2DG176300 Rh2DG176400 Rh2DG176500 Rh2DG176600 Rh2DG176900 Rh2DG177100 Rh2DG177200 Rh2DG177800 Rh2DG177900 Rh2DG178700 Rh2DG178800 Rh2DG555700 Rh2DG643700 Rh2DG646300 Rh3AG235600 Rh3AG235900 Rh3AG236200 Rh3AG266700 Rh3AG266900 Rh3BG216200 Rh3BG269100 Rh3CG212700 Rh3CG265800 Rh4AG168900 Rh4BG165500 Rh4CG180300 Rh5BG261500 Rh6AG286300 Rh6BG287500 Rh6CG288600 Rh7AG022600
rosa_wichuraiana Rw0G008240 Rw0G008250 Rw0G013190 Rw0G013200 Rw2G013230 Rw2G013240 Rw2G013270 Rw2G013280 Rw2G013290 Rw2G013300 Rw2G013330 Rw2G013340 Rw2G013350 Rw2G013360 Rw2G013370 Rw2G013380 Rw2G013410 Rw2G013570 Rw2G044120 Rw2G051370 Rw2G051590 Rw3G017160 Rw3G021150 Rw3G021160 Rw3G023730 Rw3G023740 Rw4G014030 Rw6G024590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1640
AatII GACGTC 1 cut(s) 111
AccB1I GGYRCC 1 cut(s) 847
AccB7I CCANNNNNTGG 2 cut(s) 1671, 1825
AccBSI CCGCTC 1 cut(s) 689
AciI CCGC 4 cut(s) 500, 687, 1209, 1526
AclWI GGATC 6 cut(s) 189, 195, 1384, 1397, 1449, 1738
AcoI YGGCCR 5 cut(s) 3, 81, 2069, 2214, 2514
AcsI RAATTY 6 cut(s) 755, 893, 980, 1025, 1789, 1894
AcuI CTGAAG 9 cut(s) 594, 662, 920, 1398, 1671, 1866, 2217, 2305, 2361
AcyI GRCGYC 1 cut(s) 108
AdeI CACNNNGTG 1 cut(s) 2509
AfaI GTAC 8 cut(s) 293, 299, 591, 816, 1851, 1878, 1912, 2248
AfiI CCNNNNNNNGG 5 cut(s) 229, 835, 1671, 1825, 2495
AflII CTTAAG 1 cut(s) 2365
Alw21I GWGCWC 1 cut(s) 246
Alw26I GTCTC 8 cut(s) 218, 343, 377, 467, 1274, 1592, 1997, 2379
AlwI GGATC 6 cut(s) 189, 195, 1384, 1397, 1449, 1738
AlwNI CAGNNNCTG 3 cut(s) 593, 1193, 1772
AoxI GGCC 6 cut(s) 3, 81, 626, 2069, 2214, 2514
ApeKI GCWGC 6 cut(s) 32, 886, 1097, 1400, 2361, 2407
ApoI RAATTY 6 cut(s) 755, 893, 980, 1025, 1789, 1894
Asp700I GAANNNNTTC 1 cut(s) 1290
AspA2I CCTAGG 1 cut(s) 1363
AspS9I GGNCC 3 cut(s) 626, 1251, 1612
AsuC2I CCSGG 5 cut(s) 28, 693, 845, 1617, 2268
AsuHPI GGTGA 5 cut(s) 514, 1471, 2158, 2363, 2462
AsuII TTCGAA 1 cut(s) 1030
AvaII GGWCC 2 cut(s) 1251, 1612
AvrII CCTAGG 1 cut(s) 1363
BaeI ACNNNNGTAYC 4 cut(s) 1531, 1564, 1670, 1703
BalI TGGCCA 5 cut(s) 5, 83, 2071, 2216, 2516
BamHI GGATCC 1 cut(s) 1389
BanI GGYRCC 1 cut(s) 847
BanII GRGCYC 2 cut(s) 60, 246
BarI GAAGNNNNNNTAC 6 cut(s) 281, 313, 582, 614, 1864, 1896
BbsI GAAGAC 4 cut(s) 165, 419, 1659, 2349
Bbv12I GWGCWC 1 cut(s) 246
BbvI GCAGC 6 cut(s) 19, 873, 1109, 1387, 2348, 2394
BccI CCATC 8 cut(s) 59, 368, 373, 490, 617, 1529, 1721, 1966
BcgI CGANNNNNNTGC 4 cut(s) 226, 260, 344, 378
BciVI GTATCC 2 cut(s) 1532, 1698
BcnI CCSGG 5 cut(s) 28, 693, 845, 1617, 2268
BcoDI GTCTC 8 cut(s) 218, 343, 377, 467, 1274, 1592, 1997, 2379
BfaI CTAG 5 cut(s) 227, 1364, 1493, 1781, 2078
BfmI CTRYAG 2 cut(s) 1218, 1543
BfrI CTTAAG 1 cut(s) 2365
BfuI GTATCC 2 cut(s) 1532, 1698
BglII AGATCT 1 cut(s) 1019
BisI GCNGC 7 cut(s) 33, 887, 1098, 1401, 1526, 2362, 2408
BlnI CCTAGG 1 cut(s) 1363
BlpI GCTNAGC 1 cut(s) 2159
BlsI GCNGC 7 cut(s) 34, 888, 1099, 1402, 1527, 2363, 2409
BmcAI AGTACT 1 cut(s) 299
Bme1390I CCNGG 5 cut(s) 28, 693, 845, 1617, 2268
Bme18I GGWCC 2 cut(s) 1251, 1612
BmgT120I GGNCC 3 cut(s) 626, 1251, 1612
BmiI GGNNCC 5 cut(s) 849, 1391, 1614, 2282, 2543
BmrFI CCNGG 5 cut(s) 28, 693, 845, 1617, 2268
BmrI ACTGGG 1 cut(s) 2286
BmsI GCATC 3 cut(s) 1109, 1956, 2172
BmuI ACTGGG 1 cut(s) 2286
BpiI GAAGAC 4 cut(s) 165, 419, 1659, 2349
BpmI CTGGAG 3 cut(s) 57, 1979, 2450
Bpu10I CCTNAGC 1 cut(s) 2237
Bpu1102I GCTNAGC 1 cut(s) 2159
Bpu14I TTCGAA 1 cut(s) 1030
BpuEI CTTGAG 5 cut(s) 245, 863, 989, 2270, 2377
BpuMI CCSGG 5 cut(s) 28, 693, 845, 1617, 2268
Bsa29I ATCGAT 1 cut(s) 1961
BsaBI GATNNNNATC 1 cut(s) 186
BsaHI GRCGYC 1 cut(s) 108
BsaI GGTCTC 2 cut(s) 377, 467
BsaJI CCNNGG 6 cut(s) 27, 195, 1104, 1363, 1615, 2517
BsaXI ACNNNNNCTCC 2 cut(s) 284, 314
Bsc4I CCNNNNNNNGG 5 cut(s) 229, 835, 1671, 1825, 2495
Bse1I ACTGG 6 cut(s) 444, 1308, 1443, 1857, 2133, 2281
Bse3DI GCAATG 5 cut(s) 1049, 1083, 2047, 2110, 2117
Bse8I GATNNNNATC 1 cut(s) 186
BseCI ATCGAT 1 cut(s) 1961
BseDI CCNNGG 6 cut(s) 27, 195, 1104, 1363, 1615, 2517
BseGI GGATG 6 cut(s) 384, 628, 704, 943, 1100, 1540
BseJI GATNNNNATC 1 cut(s) 186
BseLI CCNNNNNNNGG 5 cut(s) 229, 835, 1671, 1825, 2495
BseMI GCAATG 5 cut(s) 1049, 1083, 2047, 2110, 2117
BseMII CTCAG 2 cut(s) 531, 1764
BseNI ACTGG 6 cut(s) 444, 1308, 1443, 1857, 2133, 2281
BseRI GAGGAG 1 cut(s) 245
BseXI GCAGC 6 cut(s) 19, 873, 1109, 1387, 2348, 2394
BseYI CCCAGC 1 cut(s) 1563
BshFI GGCC 6 cut(s) 5, 83, 628, 2071, 2216, 2516
BshNI GGYRCC 1 cut(s) 847
BshVI ATCGAT 1 cut(s) 1961
BsiHKAI GWGCWC 1 cut(s) 246
BsiSI CCGG 5 cut(s) 27, 693, 845, 1617, 2267
BslI CCNNNNNNNGG 5 cut(s) 229, 835, 1671, 1825, 2495
BsmAI GTCTC 8 cut(s) 218, 343, 377, 467, 1274, 1592, 1997, 2379
BsnI GGCC 6 cut(s) 5, 83, 628, 2071, 2216, 2516
Bso31I GGTCTC 2 cut(s) 377, 467
Bsp119I TTCGAA 1 cut(s) 1030
Bsp1286I GDGCHC 2 cut(s) 60, 246
Bsp1407I TGTACA 2 cut(s) 814, 2246
Bsp143I GATC 9 cut(s) 181, 187, 1019, 1381, 1389, 1441, 1476, 1570, 1730
Bsp1720I GCTNAGC 1 cut(s) 2159
BspACI CCGC 4 cut(s) 500, 687, 1209, 1526
BspANI GGCC 6 cut(s) 5, 83, 628, 2071, 2216, 2516
BspCNI CTCAG 2 cut(s) 532, 1765
BspDI ATCGAT 1 cut(s) 1961
BspLI GGNNCC 5 cut(s) 849, 1391, 1614, 2282, 2543
BspPI GGATC 6 cut(s) 189, 195, 1384, 1397, 1449, 1738
BspT104I TTCGAA 1 cut(s) 1030
BspT107I GGYRCC 1 cut(s) 847
BspTI CTTAAG 1 cut(s) 2365
BspTNI GGTCTC 2 cut(s) 377, 467
BsrBI CCGCTC 1 cut(s) 689
BsrDI GCAATG 5 cut(s) 1049, 1083, 2047, 2110, 2117
BsrGI TGTACA 2 cut(s) 814, 2246
BsrI ACTGG 6 cut(s) 444, 1308, 1443, 1857, 2133, 2281
BssECI CCNNGG 6 cut(s) 27, 195, 1104, 1363, 1615, 2517
BssMI GATC 9 cut(s) 181, 187, 1019, 1381, 1389, 1441, 1476, 1570, 1730
BssNI GRCGYC 1 cut(s) 108
BssT1I CCWWGG 4 cut(s) 195, 1104, 1363, 2517
BstACI GRCGYC 1 cut(s) 108
BstAFI CTTAAG 1 cut(s) 2365
BstAPI GCANNNNNTGC 4 cut(s) 1573, 1978, 2123, 2407
BstAUI TGTACA 2 cut(s) 814, 2246
BstBI TTCGAA 1 cut(s) 1030
BstC8I GCNNGC 5 cut(s) 93, 1587, 1705, 2124, 2214
BstDEI CTNAG 7 cut(s) 540, 1357, 1577, 1773, 2159, 2237, 2254
BstEII GGTNACC 1 cut(s) 839
BstENI CCTNNNNNAGG 1 cut(s) 2493
BstF5I GGATG 6 cut(s) 384, 628, 704, 943, 1100, 1540
BstKTI GATC 9 cut(s) 184, 190, 1022, 1384, 1392, 1444, 1479, 1573, 1733
BstMAI GTCTC 8 cut(s) 218, 343, 377, 467, 1274, 1592, 1997, 2379
BstMBI GATC 9 cut(s) 181, 187, 1019, 1381, 1389, 1441, 1476, 1570, 1730
BstMWI GCNNNNNNNGC 8 cut(s) 11, 32, 584, 1573, 1582, 1978, 2123, 2407
BstNSI RCATGY 1 cut(s) 95
BstPI GGTNACC 1 cut(s) 839
BstSCI CCNGG 5 cut(s) 26, 691, 843, 1615, 2266
BstSFI CTRYAG 2 cut(s) 1218, 1543
BstV1I GCAGC 6 cut(s) 19, 873, 1109, 1387, 2348, 2394
BstV2I GAAGAC 4 cut(s) 165, 419, 1659, 2349
BstX2I RGATCY 3 cut(s) 181, 1019, 1389
BstYI RGATCY 3 cut(s) 181, 1019, 1389
Bsu15I ATCGAT 1 cut(s) 1961
BsuI GTATCC 2 cut(s) 1532, 1698
BsuRI GGCC 6 cut(s) 5, 83, 628, 2071, 2216, 2516
BsuTUI ATCGAT 1 cut(s) 1961
BtgZI GCGATG 2 cut(s) 110, 533
BtsCI GGATG 6 cut(s) 384, 628, 704, 943, 1100, 1540
BtsI GCAGTG 1 cut(s) 480
BtsIMutI CAGTG 6 cut(s) 480, 780, 1194, 1436, 1552, 2140
Cac8I GCNNGC 5 cut(s) 93, 1587, 1705, 2124, 2214
CaiI CAGNNNCTG 3 cut(s) 593, 1193, 1772
Cfr13I GGNCC 3 cut(s) 626, 1251, 1612
ClaI ATCGAT 1 cut(s) 1961
CseI GACGC 1 cut(s) 109
Csp6I GTAC 8 cut(s) 292, 298, 590, 815, 1850, 1877, 1911, 2247
CspCI CAANNNNNGTGG 2 cut(s) 2061, 2096
CviAII CATG 9 cut(s) 92, 1094, 1568, 1696, 1756, 1978, 2014, 2067, 2380
CviQI GTAC 8 cut(s) 292, 298, 590, 815, 1850, 1877, 1911, 2247
DdeI CTNAG 7 cut(s) 540, 1357, 1577, 1773, 2159, 2237, 2254
DpnI GATC 9 cut(s) 183, 189, 1021, 1383, 1391, 1443, 1478, 1572, 1732
DpnII GATC 9 cut(s) 181, 187, 1019, 1381, 1389, 1441, 1476, 1570, 1730
DraI TTTAAA 1 cut(s) 859
DraIII CACNNNGTG 1 cut(s) 2509
EaeI YGGCCR 5 cut(s) 3, 81, 2069, 2214, 2514
Ecl136II GAGCTC 1 cut(s) 244
Eco130I CCWWGG 4 cut(s) 195, 1104, 1363, 2517
Eco24I GRGCYC 2 cut(s) 60, 246
Eco31I GGTCTC 2 cut(s) 377, 467
Eco32I GATATC 1 cut(s) 1414
Eco47I GGWCC 2 cut(s) 1251, 1612
Eco53kI GAGCTC 1 cut(s) 244
Eco57I CTGAAG 9 cut(s) 594, 662, 920, 1398, 1671, 1866, 2217, 2305, 2361
Eco91I GGTNACC 1 cut(s) 839
EcoICRI GAGCTC 1 cut(s) 244
EcoNI CCTNNNNNAGG 1 cut(s) 2493
EcoO109I RGGNCCY 1 cut(s) 1612
EcoO65I GGTNACC 1 cut(s) 839
EcoRI GAATTC 1 cut(s) 980
EcoRV GATATC 1 cut(s) 1414
EcoT14I CCWWGG 4 cut(s) 195, 1104, 1363, 2517
EcoT22I ATGCAT 2 cut(s) 1051, 2068
EcoT38I GRGCYC 2 cut(s) 60, 246
ErhI CCWWGG 4 cut(s) 195, 1104, 1363, 2517
FaeI CATG 9 cut(s) 95, 1097, 1571, 1699, 1759, 1981, 2017, 2070, 2383
FatI CATG 9 cut(s) 91, 1093, 1567, 1695, 1755, 1977, 2013, 2066, 2379
Fnu4HI GCNGC 7 cut(s) 33, 887, 1098, 1401, 1526, 2362, 2408
FokI GGATG 6 cut(s) 391, 635, 711, 950, 1087, 1547
FriOI GRGCYC 2 cut(s) 60, 246
Fsp4HI GCNGC 7 cut(s) 33, 887, 1098, 1401, 1526, 2362, 2408
FspBI CTAG 5 cut(s) 227, 1364, 1493, 1781, 2078
GluI GCNGC 7 cut(s) 33, 887, 1098, 1401, 1526, 2362, 2408
GsaI CCCAGC 1 cut(s) 1567
GsuI CTGGAG 3 cut(s) 57, 1979, 2450
HaeIII GGCC 6 cut(s) 5, 83, 628, 2071, 2216, 2516
HapII CCGG 5 cut(s) 27, 693, 845, 1617, 2267
HgaI GACGC 1 cut(s) 109
Hin1I GRCGYC 1 cut(s) 108
Hin1II CATG 9 cut(s) 95, 1097, 1571, 1699, 1759, 1981, 2017, 2070, 2383
HindIII AAGCTT 3 cut(s) 576, 2031, 2156
HpaII CCGG 5 cut(s) 27, 693, 845, 1617, 2267
HphI GGTGA 5 cut(s) 514, 1471, 2158, 2363, 2462
Hpy166II GTNNAC 4 cut(s) 283, 1436, 1911, 2089
Hpy188I TCNGA 8 cut(s) 790, 900, 958, 1024, 1279, 1651, 1774, 1885
Hpy8I GTNNAC 4 cut(s) 283, 1436, 1911, 2089
HpyAV CCTTC 8 cut(s) 895, 902, 1510, 1693, 1840, 1890, 2489, 2491
HpyCH4IV ACGT 2 cut(s) 108, 1913
HpyF10VI GCNNNNNNNGC 8 cut(s) 11, 32, 584, 1573, 1582, 1978, 2123, 2407
HpyF3I CTNAG 7 cut(s) 540, 1357, 1577, 1773, 2159, 2237, 2254
HpySE526I ACGT 2 cut(s) 108, 1913
Hsp92I GRCGYC 1 cut(s) 108
Hsp92II CATG 9 cut(s) 95, 1097, 1571, 1699, 1759, 1981, 2017, 2070, 2383
Kzo9I GATC 9 cut(s) 181, 187, 1019, 1381, 1389, 1441, 1476, 1570, 1730
LmnI GCTCC 4 cut(s) 435, 694, 1674, 2547
Lsp1109I GCAGC 6 cut(s) 19, 873, 1109, 1387, 2348, 2394
LweI GCATC 3 cut(s) 1109, 1956, 2172
MaeI CTAG 5 cut(s) 227, 1364, 1493, 1781, 2078
MaeII ACGT 2 cut(s) 108, 1913
MaeIII GTNAC 5 cut(s) 839, 1009, 1507, 1558, 1858
MalI GATC 9 cut(s) 183, 189, 1021, 1383, 1391, 1443, 1478, 1572, 1732
MbiI CCGCTC 1 cut(s) 689
MboI GATC 9 cut(s) 181, 187, 1019, 1381, 1389, 1441, 1476, 1570, 1730
MflI RGATCY 3 cut(s) 181, 1019, 1389
MhlI GDGCHC 2 cut(s) 60, 246
MlsI TGGCCA 5 cut(s) 5, 83, 2071, 2216, 2516
MluNI TGGCCA 5 cut(s) 5, 83, 2071, 2216, 2516
MlyI GAGTC 5 cut(s) 345, 953, 1034, 1233, 1433
MmeI TCCRAC 2 cut(s) 595, 626
Mox20I TGGCCA 5 cut(s) 5, 83, 2071, 2216, 2516
Mph1103I ATGCAT 2 cut(s) 1051, 2068
MroXI GAANNNNTTC 1 cut(s) 1290
MscI TGGCCA 5 cut(s) 5, 83, 2071, 2216, 2516
MseI TTAA 9 cut(s) 72, 656, 858, 1245, 1839, 1889, 2328, 2366, 2376
MslI CAYNNNNRTG 1 cut(s) 2510
Msp20I TGGCCA 5 cut(s) 5, 83, 2071, 2216, 2516
MspA1I CMGCKG 1 cut(s) 14
MspCI CTTAAG 1 cut(s) 2365
MspI CCGG 5 cut(s) 27, 693, 845, 1617, 2267
MspR9I CCNGG 5 cut(s) 28, 693, 845, 1617, 2268
MwoI GCNNNNNNNGC 8 cut(s) 11, 32, 584, 1573, 1582, 1978, 2123, 2407
NciI CCSGG 5 cut(s) 28, 693, 845, 1617, 2268
NdeII GATC 9 cut(s) 181, 187, 1019, 1381, 1389, 1441, 1476, 1570, 1730
NlaIII CATG 9 cut(s) 95, 1097, 1571, 1699, 1759, 1981, 2017, 2070, 2383
NlaIV GGNNCC 5 cut(s) 849, 1391, 1614, 2282, 2543
NmeAIII GCCGAG 1 cut(s) 533
NmuCI GTSAC 3 cut(s) 1009, 1507, 1858
NsiI ATGCAT 2 cut(s) 1051, 2068
NspI RCATGY 1 cut(s) 95
NspV TTCGAA 1 cut(s) 1030
PaeI GCATGC 1 cut(s) 95
PdmI GAANNNNTTC 1 cut(s) 1290
PfeI GAWTC 5 cut(s) 137, 1267, 1274, 1769, 2143
PflFI GACNNNGTC 1 cut(s) 1423
PflMI CCANNNNNTGG 2 cut(s) 1671, 1825
PfoI TCCNGGA 1 cut(s) 691
PkrI GCNGC 7 cut(s) 34, 888, 1099, 1402, 1527, 2363, 2409
PleI GAGTC 5 cut(s) 345, 953, 1034, 1233, 1432
PpsI GAGTC 5 cut(s) 345, 953, 1034, 1233, 1432
PpuMI RGGWCCY 1 cut(s) 1612
PsiI TTATAA 1 cut(s) 1640
Psp124BI GAGCTC 1 cut(s) 246
Psp5II RGGWCCY 1 cut(s) 1612
PspEI GGTNACC 1 cut(s) 839
PspFI CCCAGC 1 cut(s) 1563
PspN4I GGNNCC 5 cut(s) 849, 1391, 1614, 2282, 2543
PspPI GGNCC 3 cut(s) 626, 1251, 1612
PspPPI RGGWCCY 1 cut(s) 1612
PstNI CAGNNNCTG 3 cut(s) 593, 1193, 1772
PsuI RGATCY 3 cut(s) 181, 1019, 1389
PsyI GACNNNGTC 1 cut(s) 1423
PvuII CAGCTG 1 cut(s) 14
RsaI GTAC 8 cut(s) 293, 299, 591, 816, 1851, 1878, 1912, 2248
RsaNI GTAC 8 cut(s) 292, 298, 590, 815, 1850, 1877, 1911, 2247
RseI CAYNNNNRTG 1 cut(s) 2510
SacI GAGCTC 1 cut(s) 246
SaqAI TTAA 9 cut(s) 72, 656, 858, 1245, 1839, 1889, 2328, 2366, 2376
SatI GCNGC 7 cut(s) 33, 887, 1098, 1401, 1526, 2362, 2408
Sau3AI GATC 9 cut(s) 181, 187, 1019, 1381, 1389, 1441, 1476, 1570, 1730
Sau96I GGNCC 3 cut(s) 626, 1251, 1612
ScaI AGTACT 1 cut(s) 299
SchI GAGTC 5 cut(s) 345, 953, 1034, 1233, 1433
ScrFI CCNGG 5 cut(s) 28, 693, 845, 1617, 2268
SduI GDGCHC 2 cut(s) 60, 246
SfaNI GCATC 3 cut(s) 1109, 1956, 2172
SfcI CTRYAG 2 cut(s) 1218, 1543
SfuI TTCGAA 1 cut(s) 1030
SinI GGWCC 2 cut(s) 1251, 1612
SmiMI CAYNNNNRTG 1 cut(s) 2510
SmlI CTYRAG 6 cut(s) 260, 878, 968, 2285, 2365, 2392
SmoI CTYRAG 6 cut(s) 260, 878, 968, 2285, 2365, 2392
SphI GCATGC 1 cut(s) 95
SsiI CCGC 4 cut(s) 500, 687, 1209, 1526
SspI AATATT 2 cut(s) 769, 2023
SspMI CTAG 5 cut(s) 227, 1364, 1493, 1781, 2078
SstI GAGCTC 1 cut(s) 246
StyD4I CCNGG 5 cut(s) 26, 691, 843, 1615, 2266
StyI CCWWGG 4 cut(s) 195, 1104, 1363, 2517
TaiI ACGT 2 cut(s) 111, 1916
TaqI TCGA 9 cut(s) 114, 135, 354, 1030, 1265, 1961, 2273, 2304, 2493
TatI WGTACW 4 cut(s) 291, 297, 814, 2246
TauI GCSGC 1 cut(s) 1528
TfiI GAWTC 5 cut(s) 137, 1267, 1274, 1769, 2143
Tru1I TTAA 9 cut(s) 72, 656, 858, 1245, 1839, 1889, 2328, 2366, 2376
Tru9I TTAA 9 cut(s) 72, 656, 858, 1245, 1839, 1889, 2328, 2366, 2376
TscAI CASTG 6 cut(s) 487, 787, 1194, 1443, 1552, 2140
TseFI GTSAC 3 cut(s) 1009, 1507, 1858
TseI GCWGC 6 cut(s) 32, 886, 1097, 1400, 2361, 2407
Tsp45I GTSAC 3 cut(s) 1009, 1507, 1858
TspRI CASTG 6 cut(s) 487, 787, 1194, 1443, 1552, 2140
Tth111I GACNNNGTC 1 cut(s) 1423
Van91I CCANNNNNTGG 2 cut(s) 1671, 1825
Vha464I CTTAAG 1 cut(s) 2365
VpaK11BI GGWCC 2 cut(s) 1251, 1612
XagI CCTNNNNNAGG 1 cut(s) 2493
XapI RAATTY 6 cut(s) 755, 893, 980, 1025, 1789, 1894
XceI RCATGY 1 cut(s) 95
XmaJI CCTAGG 1 cut(s) 1363
XmnI GAANNNNTTC 1 cut(s) 1290
XspI CTAG 5 cut(s) 227, 1364, 1493, 1781, 2078
ZraI GACGTC 1 cut(s) 109
ZrmI AGTACT 1 cut(s) 299
Zsp2I ATGCAT 2 cut(s) 1051, 2068
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.