Rh2DG176500

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0000039
Physical Location & Seq
Forward (+)
15513734 .. 15519230
5497 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG176500.1

Sequence Viewer

Length: 2505 bp
ATGGCTTCTGAGGGCGCTACGAAATCGAGCTCAGCGGCCCTCGGCTCCGTCGTCGTGGTTGGCTCCGCTGGAGCCGTGGCATTGATCGGAGGAACTGGTTTGCCAGTAACATTTTGGACGCTGTGTGATTCTGTTAAGCAAATGAAGGATATACTGATGTTCAAACCCCAACTAAGGGATATCAAATCCACTTTGGACTCTTTGGAACCACTGATCAAAGAGATGGCAGAGTGTGAGAAGGAATTGGATGGTCAAGAGGAGGAGCTAGAGAATCTTAGAGTGGAAATGGAGGGAGGAGTGGAACTTATCCGCAAGTGCTCGAAAGCAAGCAGGTTCAAGCAGTATAAGTACGGCAGCAGGCTTGTTGAATTCAGCAAGTCACTTCAGAGGCTGTTGGGGATATTGAAAGTGCAGGGAATAAGGGATGTGAAGAAGGCATTGGTTTCGGTGAGAAATATCGAGAATGTGGTGCATAGAATTGAAGGAAATTGTGTGACACAGAACAATCAGTCTGAAAAAATTGCGGGGTGGTGTGCTGTGCCTGAGCCTCCAACGATTACTGTTGGATTGGATGTGCCTTTGTGGGAATTGAAGAAGAAGCTTTTTACTGACGGAGTATCGATGGTGATAATTACTGCTCCTGGAGGATGTGGAAAAACTACATTGGCAACAAAGTTTTGCAAGGATGCAGAAGTCAAAGATAAATTCAAGAGCAATGTCTTCTTTGTTACTGTTTCAAAGAAGCCCAACTTATACCTAATTGTACAAGAGCTATATCATGGAACGGGTGCCCAGGTCCCCGATTTCCAAAATGAAGCAACTGCAGTTAGCTGGCTGCAGCAATTTCTGAAGCAAACAGGACAAAATCCTATGTTGTTGGTCCTGGATGATGTCTGGGCAGGATCAGAATTCATTCTTGAGAAGTTTGATGAATTAAAGATGTCAGATTACAAGATATTGGTGACATCAAGGTCTGCTTTTCCAAGATTTGGTTCTCCATATTATTTAGAATGTTTGAACAACAAAGATGCAATGGCACTTTTTCACCATGCAGCATCCTTAGGTGATAGGAGCTCTTATATTTCAGAAGATCTTCAGAGAAAGATAGTAGAACGTTGTATGGGATTTCCACTTGCAATTACAGTTGTCGGAAGATCACTTTGCGGGCAGCCAATAGAGATTTGGCAAAAAAGAGTACTGGAATGGTCTAGAGGTTGTTCTGTACTTGAGTCAGAACATGATTTGCTTGATTGCCTCCAAAGAAGCTTAGATGCTTTGGATGAAGAGAAGTCTCTTATCAGGGAATGCTTCATAGACCTTGGTTTATTTCCTGAAGATAAAAGAATCTCAGCTACTGTCCTCATTGATATGTGGGCAGAGTTATATGGCATAAATGAGGACATTTCGTCCATTGTGAACCTCTATGAACTTGCCTCTCGGAGTTTGGTTAATCTTGTACTCACAAGGAATGACAAGGATGGCGATGGCTATTACACTGAACACTTTGTTACACAGCATGACATGCTTAGGGAGTTGGCTATCCAACAGATTAGTCGGGAACCACTACAACAGAGAATGAGACTTATGATTGACATAAGTGGAGACAACTTTCCTATGTGGTGGAAAGAACAAAAGTATCAATCCCTCATAGCTCGCCTTGTATCTATCTCAACAGACGAAGAGTTCTCATCTAAATGGCAAAACTTGCCACTACCAGAAGCAGAGGTTTTGATTTTGAACTTTCAGACCAAGTATTATGCCTTACCTGAGTTTGTGGAGGAAATGAATAAACTGAAGACTCTAGTACTCACAAGTTCAGGTATCGGTCCTGCTGAGTTGAATAACTTTCAACTAGTTGATTCGAAACCAAATCTAAAGAGAATCAGACTAGAACGAATTTCATTTCCTTCATTAAGCAGGTATCCTATACAGTTGAAAAGTTTGAAAAAGATTTCTCTATTCATGTGTAGCGTTGGCCAAGGTTTTGGTAACGGTTCCATACATATATCAGAGGCGTTTCCAAATCTTGTTGAAATGAATATTGATTACTGCAATGATTTGGTGGAGTTGCCTGCCGATCTCTGTGACCTTATTCAACTGAAGAAGCTCAGTGTCACCAATTCTCATAAGCTCTCTGTCTTACCTGAAGACATTGGAAATCTAGTAAATTTAGAGCTGCTGAGGCTTAGGTGTTGTACAGACTTGTCAGAGTTACCCGGCTCGATTAGGAAGCTCAAGAACTTAAACTTGCTTGACATATCGAATTGCTTTAGCCTTAAGGAATTGCCTGAAGACATTGGAGAAATGTGCAGTTTAGAGAAGCTCAACATGAGACAGTGCTCGAGATTGAATGAGTTGCCTTCATCAGTTGTGGATCTTGAAAAGTTAATGGAAGTGACATGTGATGAAGTGACAGAAATCTTATGGGAGCCTTGCTTGCCGTATCTCAGTAACATACACATAAAGGTTTTGAAAGAAGATATCAACCTGAATTGGCTCCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

834

Amino Acids

94.29

Weight (kDa)

5.3

Isoelectric Point (pI)

42.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 28 - 152 5.3e-19 Arabidopsis broad-spectrum mildew resistance protein RPW8
NB-ARC PF00931 196 - 350 1.4e-15 NB-ARC domain
AAA_22 PF13401 207 - 302 6.9e-07 AAA domain
LRR_14 PF23598 673 - 756 6.6e-09 Leucine-rich repeat region
LRR_14 PF23598 758 - 806 6.6e-07 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000039)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66890 AT5G66900 AT5G66900 AT5G66900 AT5G66910
fragaria_vesca FvH4_1g15090 FvH4_1g15120 FvH4_1g15130 FvH4_1g15140 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15170 FvH4_1g15171 FvH4_1g15171 FvH4_1g15200 FvH4_1g15200 FvH4_1g15200 FvH4_1g15210 FvH4_1g15210 FvH4_1g15210 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15230 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15500 FvH4_1g15500 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_2g19760 FvH4_3g12820 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_6g18970 FvH4_6g42370 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g50100 FvH4_6g50100 FvH4_6g50110 FvH4_6g50130 FvH4_6g50140 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50330
malus_domestica MD00G1053700.v1.1 MD02G1164300.v1.1 MD02G1164400.v1.1 MD02G1164700.v1.1 MD02G1164800.v1.1 MD02G1165300.v1.1 MD02G1165400.v1.1 MD02G1165500.v1.1 MD02G1165600.v1.1 MD02G1165900.v1.1 MD02G1166000.v1.1 MD02G1166100.v1.1 MD02G1166300.v1.1 MD08G1084900.v1.1 MD15G1263100.v1.1 MD15G1263300.v1.1 MD15G1276900.v1.1 MD15G1277200.v1.1 MD15G1277400.v1.1 MD15G1277700.v1.1 MD15G1277800.v1.1 MD15G1278000.v1.1 MD15G1278100.v1.1 MD15G1278400.v1.1 MD15G1278600.v1.1
prunus_persica Prupe.7G138500_v2.0.a1 Prupe.7G138500_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138700_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138900_v2.0.a1 Prupe.7G139100_v2.0.a1 Prupe.7G139200_v2.0.a1 Prupe.7G139500_v2.0.a1 Prupe.7G139600_v2.0.a1 Prupe.7G139700_v2.0.a1 Prupe.7G139800_v2.0.a1
pyrus_communis pycom02g13150 pycom02g13160 pycom02g13190 pycom02g13200 pycom02g13220 pycom02g13230 pycom02g13250 pycom02g13260 pycom02g13270 pycom02g13300 pycom02g13310 pycom02g13330 pycom15g23980 pycom15g23990 pycom15g24030 pycom15g24040 pycom15g24050 pycom15g24070 pycom15g24090 pycom15g24160
rosa_chinensis RchiOBHm_Chr1g0326431 RchiOBHm_Chr1g0326451 RchiOBHm_Chr2g0104121 RchiOBHm_Chr2g0104151 RchiOBHm_Chr2g0104171 RchiOBHm_Chr2g0104181 RchiOBHm_Chr2g0104191 RchiOBHm_Chr2g0104201 RchiOBHm_Chr2g0104211 RchiOBHm_Chr2g0104241 RchiOBHm_Chr2g0104261 RchiOBHm_Chr2g0104281 RchiOBHm_Chr2g0104311 RchiOBHm_Chr2g0104331 RchiOBHm_Chr2g0104361 RchiOBHm_Chr2g0104381 RchiOBHm_Chr2g0104391 RchiOBHm_Chr2g0104431 RchiOBHm_Chr2g0104441 RchiOBHm_Chr2g0104541 RchiOBHm_Chr2g0104611 RchiOBHm_Chr2g0104741 RchiOBHm_Chr2g0104761 RchiOBHm_Chr2g0158291 RchiOBHm_Chr2g0170371 RchiOBHm_Chr2g0170631 RchiOBHm_Chr3g0473971 RchiOBHm_Chr3g0480491 RchiOBHm_Chr3g0480541 RchiOBHm_Chr3g0480561 RchiOBHm_Chr3g0485281 RchiOBHm_Chr3g0485291 RchiOBHm_Chr4g0411541 RchiOBHm_Chr6g0285471
rosa_laevigata RLG00000008347 RLG00000012637 RLG00000016961 RLG00000017293 RLG00000017305 RLG00000017308 RLG00000017312 RLG00000017314 RLG00000017317 RLG00000017318 RLG00000017319 RLG00000017321 RLG00000017322 RLG00000017323 RLG00000017324 RLG00000017325 RLG00000017327 RLG00000017328 RLG00000017329 RLG00000017330 RLG00000017334 RLG00000020833 RLG00000021073 RLG00000021950 RLG00000021971 RLG00000023154 RLG00000023157 RLG00000023467 RLG00000023468 RLG00000023525 RLG00000023963 RLG00000033265 RLG00000033267 RLG00000033268
rosa_multiflora Rmu_co8053082.1_g000001 Rmu_co8210358.1_g000001 Rmu_co8331661.1_g000001 Rmu_co8477427.1_g000002 Rmu_co8483217.1_g000001 Rmu_sc0000284.1_g000004 Rmu_sc0000676.1_g000017 Rmu_sc0000676.1_g000019 Rmu_sc0000676.1_g000021 Rmu_sc0002205.1_g000038 Rmu_sc0002205.1_g000041 Rmu_sc0002316.1_g000010 Rmu_sc0002316.1_g000012 Rmu_sc0002316.1_g000040 Rmu_sc0002316.1_g000071 Rmu_sc0002316.1_g000072 Rmu_sc0002316.1_g000095 Rmu_sc0002316.1_g000102 Rmu_sc0002414.1_g000018 Rmu_sc0002576.1_g000002 Rmu_sc0002576.1_g000003 Rmu_sc0002687.1_g000010 Rmu_sc0002687.1_g000017 Rmu_sc0003660.1_g000001 Rmu_sc0003787.1_g000014 Rmu_sc0003787.1_g000020 Rmu_sc0003787.1_g000024 Rmu_sc0003787.1_g000029 Rmu_sc0004059.1_g000026 Rmu_sc0005137.1_g000021 Rmu_sc0006550.1_g000001 Rmu_sc0006561.1_g000025 Rmu_sc0006561.1_g000033 Rmu_sc0007704.1_g000005 Rmu_sc0008904.1_g000005 Rmu_sc0010270.1_g000002 Rmu_sc0010307.1_g000001 Rmu_sc0011601.1_g000013 Rmu_sc0012694.1_g000005 Rmu_sc0013101.1_g000008 Rmu_sc0019029.1_g000001 Rmu_sc0019728.1_g000001 Rmu_sc0025829.1_g000001 Rmu_sc0025829.1_g000002 Rmu_ssc0000257.1_g000007 Rmu_ssc0000257.1_g000008
rosa_roxburghii Rroxscaffold_1G00021710 Rroxscaffold_1G00049350 Rroxscaffold_1G00049360 Rroxscaffold_2G00081160 Rroxscaffold_2G00081360 Rroxscaffold_2G00081390 Rroxscaffold_2G00091540 Rroxscaffold_2G00138790 Rroxscaffold_2G00138800 Rroxscaffold_2G00138920 Rroxscaffold_2G00139000 Rroxscaffold_2G00139010 Rroxscaffold_2G00139020 Rroxscaffold_2G00139030 Rroxscaffold_2G00139040 Rroxscaffold_2G00139050 Rroxscaffold_2G00139060 Rroxscaffold_2G00139070 Rroxscaffold_2G00139090 Rroxscaffold_2G00139100 Rroxscaffold_3G00257470 Rroxscaffold_4G00318040 Rroxscaffold_4G00323740 Rroxscaffold_4G00323750 Rroxscaffold_5G00356140 Rroxscaffold_6G00401280 Rroxscaffold_6G00401290 Rroxscaffold_7G00183120
rosa_rugosa Rorug01G0060500 Rorug01G0060900 Rorug02G0119500 Rorug02G0119600 Rorug02G0119700 Rorug02G0119700 Rorug02G0119700 Rorug02G0119800.1 Rorug02G0119900.1 Rorug02G0120000 Rorug02G0120100 Rorug02G0120200 Rorug02G0120300 Rorug02G0120400 Rorug02G0120500 Rorug02G0120700 Rorug02G0120700 Rorug02G0122300 Rorug02G0122300 Rorug02G0122400.1 Rorug02G0466700 Rorug02G0466700 Rorug02G0466800 Rorug02G0548600 Rorug02G0549900 Rorug02G0550000 Rorug03G0184200 Rorug04G0107900 Rorug05G0121500 Rorug06G0174800
rosa_samantha Rh1AG198400 Rh1CG074700 Rh1CG075000 Rh1CG183700 Rh2AG169700 Rh2AG169800 Rh2AG169900 Rh2AG170000 Rh2AG170300 Rh2AG170400 Rh2AG171000 Rh2AG171100 Rh2AG171600 Rh2AG172400 Rh2AG172500 Rh2AG532900 Rh2AG620000 Rh2AG622500 Rh2AG622800 Rh2AG623000 Rh2BG176200 Rh2BG176300 Rh2BG176400 Rh2BG176500 Rh2BG176600 Rh2BG176900 Rh2BG177000 Rh2BG177100 Rh2BG177200 Rh2BG177300 Rh2BG177600 Rh2BG177700 Rh2BG177900 Rh2BG178000 Rh2BG178200 Rh2BG178300 Rh2BG178500 Rh2BG179000 Rh2BG180000 Rh2BG180100 Rh2BG259200 Rh2BG546700 Rh2BG630800 Rh2BG633500 Rh2CG176500 Rh2CG177500 Rh2CG177700 Rh2DG174400 Rh2DG174600 Rh2DG174900 Rh2DG175100 Rh2DG175200 Rh2DG175300 Rh2DG175600 Rh2DG175700 Rh2DG175800 Rh2DG175900 Rh2DG176000 Rh2DG176300 Rh2DG176400 Rh2DG176500 Rh2DG176600 Rh2DG176900 Rh2DG177100 Rh2DG177200 Rh2DG177800 Rh2DG177900 Rh2DG178700 Rh2DG178800 Rh2DG555700 Rh2DG643700 Rh2DG646300 Rh3AG235600 Rh3AG235900 Rh3AG236200 Rh3AG266700 Rh3AG266900 Rh3BG216200 Rh3BG269100 Rh3CG212700 Rh3CG265800 Rh4AG168900 Rh4BG165500 Rh4CG180300 Rh5BG261500 Rh6AG286300 Rh6BG287500 Rh6CG288600 Rh7AG022600
rosa_wichuraiana Rw0G008240 Rw0G008250 Rw0G013190 Rw0G013200 Rw2G013230 Rw2G013240 Rw2G013270 Rw2G013280 Rw2G013290 Rw2G013300 Rw2G013330 Rw2G013340 Rw2G013350 Rw2G013360 Rw2G013370 Rw2G013380 Rw2G013410 Rw2G013570 Rw2G044120 Rw2G051370 Rw2G051590 Rw3G017160 Rw3G021150 Rw3G021160 Rw3G023730 Rw3G023740 Rw4G014030 Rw6G024590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 970
Acc36I ACCTGC 2 cut(s) 321, 1908
AccB1I GGYRCC 1 cut(s) 788
AccB7I CCANNNNNTGG 1 cut(s) 989
AciI CCGC 5 cut(s) 35, 66, 310, 524, 1164
AclI AACGTT 1 cut(s) 1114
AclWI GGATC 2 cut(s) 910, 2382
AcoI YGGCCR 1 cut(s) 1975
AcsI RAATTY 5 cut(s) 368, 704, 908, 1896, 2167
AcuI CTGAAG 8 cut(s) 368, 869, 1079, 1353, 1814, 2120, 2166, 2310
AfaI GTAC 7 cut(s) 350, 765, 1197, 1224, 1458, 1806, 2197
AfiI CCNNNNNNNGG 3 cut(s) 174, 175, 989
AflII CTTAAG 1 cut(s) 2276
AflIII ACRYGT 1 cut(s) 2399
AhdI GACNNNNNGTC 1 cut(s) 1405
AhlI ACTAGT 1 cut(s) 1852
AjnI CCWGG 3 cut(s) 640, 792, 882
AjuI GAANNNNNNNTTGG 4 cut(s) 422, 454, 976, 1008
Alw21I GWGCWC 4 cut(s) 32, 320, 1076, 2342
Alw26I GTCTC 4 cut(s) 1296, 1573, 1596, 2326
AlwI GGATC 2 cut(s) 910, 2382
AlwNI CAGNNNCTG 2 cut(s) 391, 1355
Ama87I CYCGRG 1 cut(s) 2341
AoxI GGCC 2 cut(s) 36, 1975
ApeKI GCWGC 6 cut(s) 354, 835, 838, 1052, 1168, 2176
ApoI RAATTY 5 cut(s) 368, 704, 908, 1896, 2167
Asp700I GAANNNNTTC 2 cut(s) 912, 1092
AspLEI GCGC 1 cut(s) 17
AspS9I GGNCC 4 cut(s) 37, 796, 880, 1826
AsuC2I CCSGG 1 cut(s) 2217
AsuHPI GGTGA 6 cut(s) 460, 637, 973, 1037, 1076, 2107
AsuII TTCGAA 1 cut(s) 1862
AvaI CYCGRG 1 cut(s) 2341
AvaII GGWCC 3 cut(s) 796, 880, 1826
AxyI CCTNAGG 1 cut(s) 1060
BaeGI GKGCMC 1 cut(s) 793
BaeI ACNNNNGTAYC 2 cut(s) 1619, 1652
BalI TGGCCA 1 cut(s) 1977
BanI GGYRCC 1 cut(s) 788
BanII GRGCYC 2 cut(s) 32, 1076
BbsI GAAGAC 4 cut(s) 712, 1802, 2154, 2298
Bbv12I GWGCWC 4 cut(s) 32, 320, 1076, 2342
BbvCI CCTCAGC 1 cut(s) 2180
BbvI GCAGC 6 cut(s) 366, 822, 850, 1064, 1180, 2163
BccI CCATC 5 cut(s) 217, 242, 616, 1472, 1478
BceAI ACGGC 3 cut(s) 59, 367, 2425
BcgI CGANNNNNNTGC 2 cut(s) 426, 460
BciT130I CCWGG 3 cut(s) 642, 794, 884
BciVI GTATCC 1 cut(s) 1932
BclI TGATCA 1 cut(s) 213
BcnI CCSGG 1 cut(s) 2217
BcoDI GTCTC 4 cut(s) 1296, 1573, 1596, 2326
BcuI ACTAGT 1 cut(s) 1852
BfaI CTAG 7 cut(s) 266, 1209, 1802, 1853, 1889, 2162, 2503
BfmI CTRYAG 2 cut(s) 822, 836
BfoI RGCGCY 1 cut(s) 18
BfrI CTTAAG 1 cut(s) 2276
BfuAI ACCTGC 2 cut(s) 321, 1908
BfuI GTATCC 1 cut(s) 1932
BglII AGATCT 1 cut(s) 1090
BisI GCNGC 7 cut(s) 36, 355, 836, 839, 1053, 1169, 2177
BlpI GCTNAGC 1 cut(s) 31
BlsI GCNGC 7 cut(s) 37, 356, 837, 840, 1054, 1170, 2178
BmcAI AGTACT 2 cut(s) 1197, 1806
Bme1390I CCNGG 4 cut(s) 642, 794, 884, 2217
Bme18I GGWCC 3 cut(s) 796, 880, 1826
BmeRI GACNNNNNGTC 1 cut(s) 1405
BmeT110I CYCGRG 1 cut(s) 2341
BmgT120I GGNCC 4 cut(s) 37, 796, 880, 1826
BmrFI CCNGG 4 cut(s) 642, 794, 884, 2217
BmsI GCATC 4 cut(s) 676, 1018, 1064, 1261
BpiI GAAGAC 4 cut(s) 712, 1802, 2154, 2298
BpmI CTGGAG 2 cut(s) 90, 663
Bpu10I CCTNAGC 4 cut(s) 543, 1526, 2180, 2186
Bpu1102I GCTNAGC 1 cut(s) 31
Bpu14I TTCGAA 1 cut(s) 1862
BpuEI CTTGAG 3 cut(s) 938, 1247, 2219
BpuMI CCSGG 1 cut(s) 2217
Bsa29I ATCGAT 1 cut(s) 620
BsaJI CCNNGG 5 cut(s) 40, 75, 792, 1318, 1978
Bsc4I CCNNNNNNNGG 3 cut(s) 174, 175, 989
Bse1I ACTGG 3 cut(s) 100, 104, 1203
Bse21I CCTNAGG 1 cut(s) 1060
Bse3DI GCAATG 3 cut(s) 721, 1038, 2059
BseBI CCWGG 3 cut(s) 642, 794, 884
BseCI ATCGAT 1 cut(s) 620
BseDI CCNNGG 5 cut(s) 40, 75, 792, 1318, 1978
BseGI GGATG 9 cut(s) 253, 430, 577, 653, 691, 892, 1055, 1285, 1483
BseLI CCNNNNNNNGG 3 cut(s) 174, 175, 989
BseMI GCAATG 3 cut(s) 721, 1038, 2059
BseMII CTCAG 8 cut(s) 45, 534, 1362, 1758, 1824, 2122, 2171, 2461
BseNI ACTGG 3 cut(s) 100, 104, 1203
BseRI GAGGAG 3 cut(s) 272, 275, 309
BseSI GKGCMC 1 cut(s) 793
BseXI GCAGC 6 cut(s) 366, 822, 850, 1064, 1180, 2163
BsgI GTGCAG 2 cut(s) 431, 2329
BshFI GGCC 2 cut(s) 38, 1977
BshNI GGYRCC 1 cut(s) 788
BshVI ATCGAT 1 cut(s) 620
BsiHKAI GWGCWC 4 cut(s) 32, 320, 1076, 2342
BsiHKCI CYCGRG 1 cut(s) 2341
BsiSI CCGG 1 cut(s) 2217
BslFI GGGAC 1 cut(s) 782
BslI CCNNNNNNNGG 3 cut(s) 174, 175, 989
BsmAI GTCTC 4 cut(s) 1296, 1573, 1596, 2326
BsmFI GGGAC 1 cut(s) 782
BsmI GAATGC 1 cut(s) 1310
BsnI GGCC 2 cut(s) 38, 1977
BsoBI CYCGRG 1 cut(s) 2341
Bsp119I TTCGAA 1 cut(s) 1862
Bsp1286I GDGCHC 5 cut(s) 32, 320, 793, 1076, 2342
Bsp1407I TGTACA 2 cut(s) 763, 2195
Bsp143I GATC 7 cut(s) 84, 213, 902, 1090, 1154, 2077, 2374
Bsp1720I GCTNAGC 1 cut(s) 31
BspACI CCGC 5 cut(s) 35, 66, 310, 524, 1164
BspANI GGCC 2 cut(s) 38, 1977
BspCNI CTCAG 8 cut(s) 44, 535, 1361, 1759, 1825, 2121, 2172, 2460
BspDI ATCGAT 1 cut(s) 620
BspMAI CTGCAG 2 cut(s) 826, 840
BspMI ACCTGC 2 cut(s) 321, 1908
BspPI GGATC 2 cut(s) 910, 2382
BspT104I TTCGAA 1 cut(s) 1862
BspT107I GGYRCC 1 cut(s) 788
BspTI CTTAAG 1 cut(s) 2276
BsrDI GCAATG 3 cut(s) 721, 1038, 2059
BsrGI TGTACA 2 cut(s) 763, 2195
BsrI ACTGG 3 cut(s) 100, 104, 1203
BssECI CCNNGG 5 cut(s) 40, 75, 792, 1318, 1978
BssMI GATC 7 cut(s) 84, 213, 902, 1090, 1154, 2077, 2374
BssT1I CCWWGG 2 cut(s) 1318, 1978
Bst2UI CCWGG 3 cut(s) 642, 794, 884
Bst4CI ACNGT 7 cut(s) 562, 733, 1144, 1357, 1932, 1994, 2337
Bst6I CTCTTC 2 cut(s) 1278, 1674
BstAFI CTTAAG 1 cut(s) 2276
BstAPI GCANNNNNTGC 2 cut(s) 1522, 1705
BstAUI TGTACA 2 cut(s) 763, 2195
BstBI TTCGAA 1 cut(s) 1862
BstC8I GCNNGC 7 cut(s) 328, 359, 833, 1166, 1654, 2073, 2438
BstDSI CCRYGG 1 cut(s) 75
BstF5I GGATG 9 cut(s) 253, 430, 577, 653, 691, 892, 1055, 1285, 1483
BstH2I RGCGCY 1 cut(s) 18
BstHHI GCGC 1 cut(s) 17
BstKTI GATC 7 cut(s) 87, 216, 905, 1093, 1157, 2080, 2377
BstMAI GTCTC 4 cut(s) 1296, 1573, 1596, 2326
BstMBI GATC 7 cut(s) 84, 213, 902, 1090, 1154, 2077, 2374
BstMWI GCNNNNNNNGC 4 cut(s) 1522, 1705, 2182, 2437
BstNI CCWGG 3 cut(s) 642, 794, 884
BstNSI RCATGY 2 cut(s) 1525, 2403
BstSCI CCNGG 4 cut(s) 640, 792, 882, 2215
BstSFI CTRYAG 2 cut(s) 822, 836
BstSLI GKGCMC 1 cut(s) 793
BstV1I GCAGC 6 cut(s) 366, 822, 850, 1064, 1180, 2163
BstV2I GAAGAC 4 cut(s) 712, 1802, 2154, 2298
BstX2I RGATCY 2 cut(s) 1090, 2374
BstXI CCANNNNNNTGG 1 cut(s) 1985
BstYI RGATCY 2 cut(s) 1090, 2374
Bsu15I ATCGAT 1 cut(s) 620
Bsu36I CCTNAGG 1 cut(s) 1060
BsuI GTATCC 1 cut(s) 1932
BsuRI GGCC 2 cut(s) 38, 1977
BsuTUI ATCGAT 1 cut(s) 620
BtgI CCRYGG 1 cut(s) 75
BtgZI GCGATG 1 cut(s) 1497
BtsCI GGATG 9 cut(s) 253, 430, 577, 653, 691, 892, 1055, 1285, 1483
BtsIMutI CAGTG 4 cut(s) 209, 1494, 2116, 2342
BveI ACCTGC 2 cut(s) 321, 1908
Cac8I GCNNGC 7 cut(s) 328, 359, 833, 1166, 1654, 2073, 2438
CaiI CAGNNNCTG 2 cut(s) 391, 1355
CfoI GCGC 1 cut(s) 17
Cfr13I GGNCC 4 cut(s) 37, 796, 880, 1826
ClaI ATCGAT 1 cut(s) 620
CseI GACGC 1 cut(s) 127
Csp6I GTAC 7 cut(s) 349, 764, 1196, 1223, 1457, 1805, 2196
CviAII CATG 8 cut(s) 779, 1049, 1238, 1517, 1522, 1963, 2329, 2400
CviQI GTAC 7 cut(s) 349, 764, 1196, 1223, 1457, 1805, 2196
DpnI GATC 7 cut(s) 86, 215, 904, 1092, 1156, 2079, 2376
DpnII GATC 7 cut(s) 84, 213, 902, 1090, 1154, 2077, 2374
DrdI GACNNNNNNGTC 1 cut(s) 970
DriI GACNNNNNGTC 1 cut(s) 1405
DseDI GACNNNNNNGTC 1 cut(s) 970
EaeI YGGCCR 1 cut(s) 1975
Eam1104I CTCTTC 2 cut(s) 1278, 1674
Eam1105I GACNNNNNGTC 1 cut(s) 1405
EarI CTCTTC 2 cut(s) 1278, 1674
Ecl136II GAGCTC 2 cut(s) 30, 1074
Eco130I CCWWGG 2 cut(s) 1318, 1978
Eco24I GRGCYC 2 cut(s) 32, 1076
Eco32I GATATC 2 cut(s) 181, 2482
Eco47I GGWCC 3 cut(s) 796, 880, 1826
Eco53kI GAGCTC 2 cut(s) 30, 1074
Eco57I CTGAAG 8 cut(s) 368, 869, 1079, 1353, 1814, 2120, 2166, 2310
Eco81I CCTNAGG 1 cut(s) 1060
Eco88I CYCGRG 1 cut(s) 2341
EcoICRI GAGCTC 2 cut(s) 30, 1074
EcoO109I RGGNCCY 1 cut(s) 796
EcoRI GAATTC 2 cut(s) 368, 908
EcoRII CCWGG 3 cut(s) 640, 792, 882
EcoRV GATATC 2 cut(s) 181, 2482
EcoT14I CCWWGG 2 cut(s) 1318, 1978
EcoT38I GRGCYC 2 cut(s) 32, 1076
ErhI CCWWGG 2 cut(s) 1318, 1978
FaeI CATG 8 cut(s) 782, 1052, 1241, 1520, 1525, 1966, 2332, 2403
FalI AAGNNNNNCTT 4 cut(s) 734, 766, 961, 993
FaqI GGGAC 1 cut(s) 782
FatI CATG 8 cut(s) 778, 1048, 1237, 1516, 1521, 1962, 2328, 2399
FauI CCCGC 2 cut(s) 517, 1157
FbaI TGATCA 1 cut(s) 213
Fnu4HI GCNGC 7 cut(s) 36, 355, 836, 839, 1053, 1169, 2177
FokI GGATG 9 cut(s) 260, 437, 584, 660, 698, 899, 1042, 1292, 1490
FriOI GRGCYC 2 cut(s) 32, 1076
Fsp4HI GCNGC 7 cut(s) 36, 355, 836, 839, 1053, 1169, 2177
FspBI CTAG 7 cut(s) 266, 1209, 1802, 1853, 1889, 2162, 2503
GlaI GCGC 1 cut(s) 16
GluI GCNGC 7 cut(s) 36, 355, 836, 839, 1053, 1169, 2177
GsuI CTGGAG 2 cut(s) 90, 663
HaeII RGCGCY 1 cut(s) 18
HaeIII GGCC 2 cut(s) 38, 1977
HapII CCGG 1 cut(s) 2217
HgaI GACGC 1 cut(s) 127
HhaI GCGC 1 cut(s) 17
Hin1II CATG 8 cut(s) 782, 1052, 1241, 1520, 1525, 1966, 2332, 2403
Hin6I GCGC 1 cut(s) 15
HinP1I GCGC 1 cut(s) 15
HindIII AAGCTT 2 cut(s) 599, 1264
HinfI GANTC 8 cut(s) 128, 197, 271, 1229, 1344, 1798, 1859, 1881
HpaII CCGG 1 cut(s) 2217
HphI GGTGA 6 cut(s) 460, 637, 973, 1037, 1076, 2107
Hpy166II GTNNAC 1 cut(s) 1417
Hpy8I GTNNAC 1 cut(s) 1417
Hpy99I CGWCG 2 cut(s) 53, 56
HpyAV CCTTC 6 cut(s) 139, 232, 427, 476, 1917, 2370
HpyCH4III ACNGT 7 cut(s) 562, 733, 1144, 1357, 1932, 1994, 2337
HpyCH4IV ACGT 1 cut(s) 1114
HpyF10VI GCNNNNNNNGC 4 cut(s) 1522, 1705, 2182, 2437
HpySE526I ACGT 1 cut(s) 1114
Hsp92II CATG 8 cut(s) 782, 1052, 1241, 1520, 1525, 1966, 2332, 2403
HspAI GCGC 1 cut(s) 15
Ksp22I TGATCA 1 cut(s) 213
Kzo9I GATC 7 cut(s) 84, 213, 902, 1090, 1154, 2077, 2374
LmnI GCTCC 8 cut(s) 50, 68, 71, 262, 643, 1071, 2428, 2502
Lsp1109I GCAGC 6 cut(s) 366, 822, 850, 1064, 1180, 2163
LweI GCATC 4 cut(s) 676, 1018, 1064, 1261
MaeI CTAG 7 cut(s) 266, 1209, 1802, 1853, 1889, 2162, 2503
MaeII ACGT 1 cut(s) 1114
MalI GATC 7 cut(s) 86, 215, 904, 1092, 1156, 2079, 2376
MboI GATC 7 cut(s) 84, 213, 902, 1090, 1154, 2077, 2374
MflI RGATCY 2 cut(s) 1090, 2374
MhlI GDGCHC 5 cut(s) 32, 320, 793, 1076, 2342
MlsI TGGCCA 1 cut(s) 1977
MluNI TGGCCA 1 cut(s) 1977
MlyI GAGTC 3 cut(s) 191, 1238, 1792
MmeI TCCRAC 4 cut(s) 544, 575, 1129, 1567
Mox20I TGGCCA 1 cut(s) 1977
MroXI GAANNNNTTC 2 cut(s) 912, 1092
MscI TGGCCA 1 cut(s) 1977
MseI TTAA 7 cut(s) 135, 935, 1449, 1913, 2243, 2277, 2387
MslI CAYNNNNRTG 2 cut(s) 1367, 1693
Msp20I TGGCCA 1 cut(s) 1977
MspA1I CMGCKG 2 cut(s) 35, 68
MspCI CTTAAG 1 cut(s) 2276
MspI CCGG 1 cut(s) 2217
MspR9I CCNGG 4 cut(s) 642, 794, 884, 2217
Mva1269I GAATGC 1 cut(s) 1310
MvaI CCWGG 3 cut(s) 642, 794, 884
MwoI GCNNNNNNNGC 4 cut(s) 1522, 1705, 2182, 2437
NciI CCSGG 1 cut(s) 2217
NdeII GATC 7 cut(s) 84, 213, 902, 1090, 1154, 2077, 2374
NlaIII CATG 8 cut(s) 782, 1052, 1241, 1520, 1525, 1966, 2332, 2403
NmeAIII GCCGAG 1 cut(s) 21
NmuCI GTSAC 7 cut(s) 378, 493, 961, 2084, 2113, 2395, 2410
NspI RCATGY 2 cut(s) 1525, 2403
NspV TTCGAA 1 cut(s) 1862
PaeR7I CTCGAG 1 cut(s) 2341
PciI ACATGT 1 cut(s) 2399
PcsI WCGNNNNNNNCGW 1 cut(s) 48
PctI GAATGC 1 cut(s) 1310
PdmI GAANNNNTTC 2 cut(s) 912, 1092
PfeI GAWTC 5 cut(s) 128, 271, 1344, 1859, 1881
PflMI CCANNNNNTGG 1 cut(s) 989
PfoI TCCNGGA 2 cut(s) 640, 882
PkrI GCNGC 7 cut(s) 37, 356, 837, 840, 1054, 1170, 2178
PleI GAGTC 3 cut(s) 191, 1237, 1792
PpsI GAGTC 3 cut(s) 191, 1237, 1792
PpuMI RGGWCCY 1 cut(s) 796
PscI ACATGT 1 cut(s) 2399
Psp124BI GAGCTC 2 cut(s) 32, 1076
Psp1406I AACGTT 1 cut(s) 1114
Psp5II RGGWCCY 1 cut(s) 796
Psp6I CCWGG 3 cut(s) 640, 792, 882
PspGI CCWGG 3 cut(s) 640, 792, 882
PspPI GGNCC 4 cut(s) 37, 796, 880, 1826
PspPPI RGGWCCY 1 cut(s) 796
PstI CTGCAG 2 cut(s) 826, 840
PstNI CAGNNNCTG 2 cut(s) 391, 1355
PsuI RGATCY 2 cut(s) 1090, 2374
RsaI GTAC 7 cut(s) 350, 765, 1197, 1224, 1458, 1806, 2197
RsaNI GTAC 7 cut(s) 349, 764, 1196, 1223, 1457, 1805, 2196
RseI CAYNNNNRTG 2 cut(s) 1367, 1693
SacI GAGCTC 2 cut(s) 32, 1076
SaqAI TTAA 7 cut(s) 135, 935, 1449, 1913, 2243, 2277, 2387
SatI GCNGC 7 cut(s) 36, 355, 836, 839, 1053, 1169, 2177
Sau3AI GATC 7 cut(s) 84, 213, 902, 1090, 1154, 2077, 2374
Sau96I GGNCC 4 cut(s) 37, 796, 880, 1826
ScaI AGTACT 2 cut(s) 1197, 1806
SchI GAGTC 3 cut(s) 191, 1238, 1792
ScrFI CCNGG 4 cut(s) 642, 794, 884, 2217
SduI GDGCHC 5 cut(s) 32, 320, 793, 1076, 2342
SfaNI GCATC 4 cut(s) 676, 1018, 1064, 1261
SfcI CTRYAG 2 cut(s) 822, 836
Sfr274I CTCGAG 1 cut(s) 2341
SfuI TTCGAA 1 cut(s) 1862
SinI GGWCC 3 cut(s) 796, 880, 1826
SlaI CTCGAG 1 cut(s) 2341
SmiMI CAYNNNNRTG 2 cut(s) 1367, 1693
SmlI CTYRAG 5 cut(s) 917, 1226, 2234, 2276, 2341
SmoI CTYRAG 5 cut(s) 917, 1226, 2234, 2276, 2341
SpeI ACTAGT 1 cut(s) 1852
SsiI CCGC 5 cut(s) 35, 66, 310, 524, 1164
SspI AATATT 1 cut(s) 2041
SspMI CTAG 7 cut(s) 266, 1209, 1802, 1853, 1889, 2162, 2503
SstI GAGCTC 2 cut(s) 32, 1076
StyD4I CCNGG 4 cut(s) 640, 792, 882, 2215
StyI CCWWGG 2 cut(s) 1318, 1978
TaaI ACNGT 7 cut(s) 562, 733, 1144, 1357, 1932, 1994, 2337
TaiI ACGT 1 cut(s) 1117
TaqI TCGA 8 cut(s) 26, 320, 459, 620, 1862, 2222, 2261, 2342
TaqII GACCGA 1 cut(s) 1814
TatI WGTACW 6 cut(s) 763, 1195, 1222, 1456, 1804, 2195
TauI GCSGC 1 cut(s) 38
TfiI GAWTC 5 cut(s) 128, 271, 1344, 1859, 1881
Tru1I TTAA 7 cut(s) 135, 935, 1449, 1913, 2243, 2277, 2387
Tru9I TTAA 7 cut(s) 135, 935, 1449, 1913, 2243, 2277, 2387
TscAI CASTG 4 cut(s) 216, 1501, 2116, 2342
TseFI GTSAC 7 cut(s) 378, 493, 961, 2084, 2113, 2395, 2410
TseI GCWGC 6 cut(s) 354, 835, 838, 1052, 1168, 2176
Tsp45I GTSAC 7 cut(s) 378, 493, 961, 2084, 2113, 2395, 2410
TspGWI ACGGA 2 cut(s) 37, 627
TspRI CASTG 4 cut(s) 216, 1501, 2116, 2342
Van91I CCANNNNNTGG 1 cut(s) 989
Vha464I CTTAAG 1 cut(s) 2276
VpaK11BI GGWCC 3 cut(s) 796, 880, 1826
XapI RAATTY 5 cut(s) 368, 704, 908, 1896, 2167
XbaI TCTAGA 1 cut(s) 1208
XceI RCATGY 2 cut(s) 1525, 2403
XcmI CCANNNNNNNNNTGG 2 cut(s) 111, 1179
XhoI CTCGAG 1 cut(s) 2341
XmnI GAANNNNTTC 2 cut(s) 912, 1092
XspI CTAG 7 cut(s) 266, 1209, 1802, 1853, 1889, 2162, 2503
ZrmI AGTACT 2 cut(s) 1197, 1806
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.