Rw2G013270

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000039
Physical Location & Seq
Forward (+)
14012507 .. 14016217
3711 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G013270.1

Sequence Viewer

Length: 2529 bp
ATGGCGGACCTCGCTGCAGGTGGTGCTATGGGAGTGCCGTTTACTCTACTGTATGAAGTGATTAAGGAAGTGGTGATCAAGACTTCGATGTTTAGACCTCTCCTTGAAGAGCTGAAGTCAAAGATAGAAGCTTTGAATCCACTGATTGCTCAGATGGACAGCTGGAATAAGGTGTTGGATCGTCCAGAGAAGGAACTTAGAGAATTTAAAGTACAAATGGAGAAGGGCCCTGAGCTTATTACCAAGTGCGCCAATCTTAGCAAGTGGAAAAGCTACAAAAAGTATAAATACAGCAACCAACTTCTTGAATTGCAGAGCTCTCTTCAGAATCTGCTGTTTATACTGCAATGGCAACAAGCAAGGGATGTGAAGGAGACCTTGGTTGGAGTAAAGAATGTAGAGACGGTGGTGCAGCGAGTGGATGTGAGTGTTGCGGAATTCAAAGAAGAAGGGATGAGTGTTATGAAGGAGACCTTGGATGAGGTAAGAAATATAGGGACGGTGGTCCGGCGGATTGATAAGAAAAGTGAGGTACAGAGCCGAATAGATTGTGACGTACCTGAACCTCCATTGGTTACAGTTGGATTGTATGTGCCTTTGAGGGAATTGAAGATGAAGCTTCTTAAGAATGATAATGTATCAATGCTTGTGCTAACTGCTCCTGGAGGCTGCGGGAAAACCACATTGGCCACAAAGTTTTGTCAAGATGAGGAAGTCAAAGGTATATTCAAGAACAATATCTTCTTCGTCACTGTTACAAAGAAGTCAACCTTCAACCTTATTGTACAAGGACTACATCAACGCAAGAGTACCGACATACCTATGTTCCAAGATGAAGTAACTGCTGTTAAGTGGCTGCGTAAATTTCTAATGGAAGAAGGACAGAACCCTGTACTGTTGGTCCTGGATGATGTTTGGTCTGAATCAAAATATATTCTTGAACAATTTGATGAAGTGAAAATGCCAAATTTCAAGATTCTGGTGACATCAAGATCACAGTTTCCTGAATATGGCTCAGTTTATCATCTAAAGTCACTGAATGATAGAGATGCAATGACTCTTTTCCGCCATTCGGCATCCCTGGGAGATGGTCAATGGAATAATCTTGCTAGAGAGATAGTAGCGCCTTGTAAGGGATATCCACTTGCAATTAAAGTGGTTGGAAAATCACTTCGCGGAAAACCGATTGAGTTCTGGCGTAAAAGAGCATTGGCATGGTCTAAAGGTGATTCTATTATTGATTCTGAGACTGAAGTGCTTCTTCGCCTTCAAACCAGCTTAGATGCTCTGGATGAAAAAGAAGCTTTCATCAAAGAATGTTTTTTAGACCTTGGTTCATTTCCCCAAGACCAAAGAATTCCAGCAGCTGCTCTGATTGATATGTGGGCAGAGTTATATGAAGGTCTCGATAAAGATTTTCTGGCCATTACAAACGTGTATGAGCTTACCACCCGAAGTTTGGCTGATCTTGTAGTCACAAGGAAGGAGACAGTGGAGGATGATGACTACTACAGTGAACACTTTGTCACCCAACATGATTTGCTCAGACAGCTCGCTGTCTACCAAGCGAAACTAGACCCAAGTAAAAAACGACTGATCATTGGCAACCGTGGAGACAATCTACCTAAGTGCTTGACTGAACAGAAGCATCAACCAATTAAACCTCGCCTGTTATCCATCTCCTCTGATGAAACGTTCTCGACAAACTTACACAACATTCAACTAGCTGATGTTGAGGTTCTAGTTTTGAATTTCAACACAAAGAGTTATGCTTTACCTGAATTTGTGGAGAAAATGGTTAATCTGAAGGTTCTAATAGTCACAAATCATGGTTTCATACCTGCTGAATTGAGTAATTTTCACCTACTGGATTCTTTACCAAATCTGAAGAGAATCAGACTAGAGCGTGTTTCAATTCCTTCTATAACCAAGCATCCCATACAGTTGAAGAGTTTAAAGAAGATCTCTTTATTCATGTGCAGTATTGGTGAAGCTTTCGGCAATTGCTCCTTCCAAATTTCAAATGCATTGCCAAATTTAGAGGAACTGAATATTGACTATTGCAATGATTTGGTGAAATTGCCTGAGGAGCTCTGCAATCTGGTTCGCCTAAAGAAGCTGAGTATCACCAACTGTCATAAGCTATCTGCCTTGCCTCAAGATATTGGGAAACTGGTGAAGGTAGAAGTTTTGCGGCTAAGGTCTTGTACGGACTTGGTAAAACTTCCATGCTCAGTTAAAGACCTTAAAAGATTAGATTTTCTTGACATATCTGATTGCTTCAGCATTGTGGAGTTGCCTGAAGACATTGGTGAGCTGAGCAGTTTAAGAAAGATCAACATGAGACAGTGCTCTAGATTGCAAGAGCTCCCCTTATCAATTCTGGATCTTCAGAAACTAGAGGAAGTGATATGCGATGAAGAGACAGCAGGGCTGTGGGAACCCTTCTCATCCGGACTTTGCAACGGATTAGTAAAGGAAGTCAAAGAAGAATTCAGCCTAGATTGGCTCCAAAAGTTTCAATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

842

Amino Acids

96.13

Weight (kDa)

6.06

Isoelectric Point (pI)

38.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 2 - 137 3.3e-29 Arabidopsis broad-spectrum mildew resistance protein RPW8
NB-ARC PF00931 200 - 356 1.2e-16 NB-ARC domain
LRR_14 PF23598 696 - 759 1.7e-07 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000039)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66890 AT5G66900 AT5G66900 AT5G66900 AT5G66910
fragaria_vesca FvH4_1g15090 FvH4_1g15120 FvH4_1g15130 FvH4_1g15140 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15170 FvH4_1g15171 FvH4_1g15171 FvH4_1g15200 FvH4_1g15200 FvH4_1g15200 FvH4_1g15210 FvH4_1g15210 FvH4_1g15210 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15230 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15500 FvH4_1g15500 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_2g19760 FvH4_3g12820 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_6g18970 FvH4_6g42370 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g50100 FvH4_6g50100 FvH4_6g50110 FvH4_6g50130 FvH4_6g50140 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50330
malus_domestica MD00G1053700.v1.1 MD02G1164300.v1.1 MD02G1164400.v1.1 MD02G1164700.v1.1 MD02G1164800.v1.1 MD02G1165300.v1.1 MD02G1165400.v1.1 MD02G1165500.v1.1 MD02G1165600.v1.1 MD02G1165900.v1.1 MD02G1166000.v1.1 MD02G1166100.v1.1 MD02G1166300.v1.1 MD08G1084900.v1.1 MD15G1263100.v1.1 MD15G1263300.v1.1 MD15G1276900.v1.1 MD15G1277200.v1.1 MD15G1277400.v1.1 MD15G1277700.v1.1 MD15G1277800.v1.1 MD15G1278000.v1.1 MD15G1278100.v1.1 MD15G1278400.v1.1 MD15G1278600.v1.1
prunus_persica Prupe.7G138500_v2.0.a1 Prupe.7G138500_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138700_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138900_v2.0.a1 Prupe.7G139100_v2.0.a1 Prupe.7G139200_v2.0.a1 Prupe.7G139500_v2.0.a1 Prupe.7G139600_v2.0.a1 Prupe.7G139700_v2.0.a1 Prupe.7G139800_v2.0.a1
pyrus_communis pycom02g13150 pycom02g13160 pycom02g13190 pycom02g13200 pycom02g13220 pycom02g13230 pycom02g13250 pycom02g13260 pycom02g13270 pycom02g13300 pycom02g13310 pycom02g13330 pycom15g23980 pycom15g23990 pycom15g24030 pycom15g24040 pycom15g24050 pycom15g24070 pycom15g24090 pycom15g24160
rosa_chinensis RchiOBHm_Chr1g0326431 RchiOBHm_Chr1g0326451 RchiOBHm_Chr2g0104121 RchiOBHm_Chr2g0104151 RchiOBHm_Chr2g0104171 RchiOBHm_Chr2g0104181 RchiOBHm_Chr2g0104191 RchiOBHm_Chr2g0104201 RchiOBHm_Chr2g0104211 RchiOBHm_Chr2g0104241 RchiOBHm_Chr2g0104261 RchiOBHm_Chr2g0104281 RchiOBHm_Chr2g0104311 RchiOBHm_Chr2g0104331 RchiOBHm_Chr2g0104361 RchiOBHm_Chr2g0104381 RchiOBHm_Chr2g0104391 RchiOBHm_Chr2g0104431 RchiOBHm_Chr2g0104441 RchiOBHm_Chr2g0104541 RchiOBHm_Chr2g0104611 RchiOBHm_Chr2g0104741 RchiOBHm_Chr2g0104761 RchiOBHm_Chr2g0158291 RchiOBHm_Chr2g0170371 RchiOBHm_Chr2g0170631 RchiOBHm_Chr3g0473971 RchiOBHm_Chr3g0480491 RchiOBHm_Chr3g0480541 RchiOBHm_Chr3g0480561 RchiOBHm_Chr3g0485281 RchiOBHm_Chr3g0485291 RchiOBHm_Chr4g0411541 RchiOBHm_Chr6g0285471
rosa_laevigata RLG00000008347 RLG00000012637 RLG00000016961 RLG00000017293 RLG00000017305 RLG00000017308 RLG00000017312 RLG00000017314 RLG00000017317 RLG00000017318 RLG00000017319 RLG00000017321 RLG00000017322 RLG00000017323 RLG00000017324 RLG00000017325 RLG00000017327 RLG00000017328 RLG00000017329 RLG00000017330 RLG00000017334 RLG00000020833 RLG00000021073 RLG00000021950 RLG00000021971 RLG00000023154 RLG00000023157 RLG00000023467 RLG00000023468 RLG00000023525 RLG00000023963 RLG00000033265 RLG00000033267 RLG00000033268
rosa_multiflora Rmu_co8053082.1_g000001 Rmu_co8210358.1_g000001 Rmu_co8331661.1_g000001 Rmu_co8477427.1_g000002 Rmu_co8483217.1_g000001 Rmu_sc0000284.1_g000004 Rmu_sc0000676.1_g000017 Rmu_sc0000676.1_g000019 Rmu_sc0000676.1_g000021 Rmu_sc0002205.1_g000038 Rmu_sc0002205.1_g000041 Rmu_sc0002316.1_g000010 Rmu_sc0002316.1_g000012 Rmu_sc0002316.1_g000040 Rmu_sc0002316.1_g000071 Rmu_sc0002316.1_g000072 Rmu_sc0002316.1_g000095 Rmu_sc0002316.1_g000102 Rmu_sc0002414.1_g000018 Rmu_sc0002576.1_g000002 Rmu_sc0002576.1_g000003 Rmu_sc0002687.1_g000010 Rmu_sc0002687.1_g000017 Rmu_sc0003660.1_g000001 Rmu_sc0003787.1_g000014 Rmu_sc0003787.1_g000020 Rmu_sc0003787.1_g000024 Rmu_sc0003787.1_g000029 Rmu_sc0004059.1_g000026 Rmu_sc0005137.1_g000021 Rmu_sc0006550.1_g000001 Rmu_sc0006561.1_g000025 Rmu_sc0006561.1_g000033 Rmu_sc0007704.1_g000005 Rmu_sc0008904.1_g000005 Rmu_sc0010270.1_g000002 Rmu_sc0010307.1_g000001 Rmu_sc0011601.1_g000013 Rmu_sc0012694.1_g000005 Rmu_sc0013101.1_g000008 Rmu_sc0019029.1_g000001 Rmu_sc0019728.1_g000001 Rmu_sc0025829.1_g000001 Rmu_sc0025829.1_g000002 Rmu_ssc0000257.1_g000007 Rmu_ssc0000257.1_g000008
rosa_roxburghii Rroxscaffold_1G00021710 Rroxscaffold_1G00049350 Rroxscaffold_1G00049360 Rroxscaffold_2G00081160 Rroxscaffold_2G00081360 Rroxscaffold_2G00081390 Rroxscaffold_2G00091540 Rroxscaffold_2G00138790 Rroxscaffold_2G00138800 Rroxscaffold_2G00138920 Rroxscaffold_2G00139000 Rroxscaffold_2G00139010 Rroxscaffold_2G00139020 Rroxscaffold_2G00139030 Rroxscaffold_2G00139040 Rroxscaffold_2G00139050 Rroxscaffold_2G00139060 Rroxscaffold_2G00139070 Rroxscaffold_2G00139090 Rroxscaffold_2G00139100 Rroxscaffold_3G00257470 Rroxscaffold_4G00318040 Rroxscaffold_4G00323740 Rroxscaffold_4G00323750 Rroxscaffold_5G00356140 Rroxscaffold_6G00401280 Rroxscaffold_6G00401290 Rroxscaffold_7G00183120
rosa_rugosa Rorug01G0060500 Rorug01G0060900 Rorug02G0119500 Rorug02G0119600 Rorug02G0119700 Rorug02G0119700 Rorug02G0119700 Rorug02G0119800.1 Rorug02G0119900.1 Rorug02G0120000 Rorug02G0120100 Rorug02G0120200 Rorug02G0120300 Rorug02G0120400 Rorug02G0120500 Rorug02G0120700 Rorug02G0120700 Rorug02G0122300 Rorug02G0122300 Rorug02G0122400.1 Rorug02G0466700 Rorug02G0466700 Rorug02G0466800 Rorug02G0548600 Rorug02G0549900 Rorug02G0550000 Rorug03G0184200 Rorug04G0107900 Rorug05G0121500 Rorug06G0174800
rosa_samantha Rh1AG198400 Rh1CG074700 Rh1CG075000 Rh1CG183700 Rh2AG169700 Rh2AG169800 Rh2AG169900 Rh2AG170000 Rh2AG170300 Rh2AG170400 Rh2AG171000 Rh2AG171100 Rh2AG171600 Rh2AG172400 Rh2AG172500 Rh2AG532900 Rh2AG620000 Rh2AG622500 Rh2AG622800 Rh2AG623000 Rh2BG176200 Rh2BG176300 Rh2BG176400 Rh2BG176500 Rh2BG176600 Rh2BG176900 Rh2BG177000 Rh2BG177100 Rh2BG177200 Rh2BG177300 Rh2BG177600 Rh2BG177700 Rh2BG177900 Rh2BG178000 Rh2BG178200 Rh2BG178300 Rh2BG178500 Rh2BG179000 Rh2BG180000 Rh2BG180100 Rh2BG259200 Rh2BG546700 Rh2BG630800 Rh2BG633500 Rh2CG176500 Rh2CG177500 Rh2CG177700 Rh2DG174400 Rh2DG174600 Rh2DG174900 Rh2DG175100 Rh2DG175200 Rh2DG175300 Rh2DG175600 Rh2DG175700 Rh2DG175800 Rh2DG175900 Rh2DG176000 Rh2DG176300 Rh2DG176400 Rh2DG176500 Rh2DG176600 Rh2DG176900 Rh2DG177100 Rh2DG177200 Rh2DG177800 Rh2DG177900 Rh2DG178700 Rh2DG178800 Rh2DG555700 Rh2DG643700 Rh2DG646300 Rh3AG235600 Rh3AG235900 Rh3AG236200 Rh3AG266700 Rh3AG266900 Rh3BG216200 Rh3BG269100 Rh3CG212700 Rh3CG265800 Rh4AG168900 Rh4BG165500 Rh4CG180300 Rh5BG261500 Rh6AG286300 Rh6BG287500 Rh6CG288600 Rh7AG022600
rosa_wichuraiana Rw0G008240 Rw0G008250 Rw0G013190 Rw0G013200 Rw2G013230 Rw2G013240 Rw2G013270 Rw2G013280 Rw2G013290 Rw2G013300 Rw2G013330 Rw2G013340 Rw2G013350 Rw2G013360 Rw2G013370 Rw2G013380 Rw2G013410 Rw2G013570 Rw2G044120 Rw2G051370 Rw2G051590 Rw3G017160 Rw3G021150 Rw3G021160 Rw3G023730 Rw3G023740 Rw4G014030 Rw6G024590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 8
Acc36I ACCTGC 2 cut(s) 8, 1849
AccI GTMKAC 1 cut(s) 1560
AccII CGCG 1 cut(s) 1176
AccIII TCCGGA 1 cut(s) 2453
AciI CCGC 7 cut(s) 5, 434, 511, 672, 1066, 1176, 2194
AclI AACGTT 1 cut(s) 1694
AclWI GGATC 2 cut(s) 186, 2394
AcoI YGGCCR 2 cut(s) 687, 1422
AcuI CTGAAG 8 cut(s) 134, 308, 1272, 1826, 1907, 2266, 2322, 2375
AfaI GTAC 7 cut(s) 213, 534, 558, 786, 811, 894, 2209
AfiI CCNNNNNNNGG 4 cut(s) 1010, 1072, 1133, 1459
AflII CTTAAG 1 cut(s) 623
AflIII ACRYGT 1 cut(s) 1434
AjnI CCWGG 3 cut(s) 661, 903, 1080
AjuI GAANNNNNNNTTGG 8 cut(s) 158, 190, 362, 394, 458, 490, 668, 700
Alw21I GWGCWC 4 cut(s) 320, 2094, 2354, 2370
Alw26I GTCTC 9 cut(s) 368, 395, 464, 1241, 1409, 1481, 1608, 2338, 2417
AlwI GGATC 2 cut(s) 186, 2394
AlwNI CAGNNNCTG 2 cut(s) 331, 1367
Aor13HI TCCGGA 1 cut(s) 2453
AoxI GGCC 3 cut(s) 226, 687, 1422
ApaI GGGCCC 1 cut(s) 230
ApeKI GCWGC 6 cut(s) 14, 412, 669, 856, 1364, 1367
Asp700I GAANNNNTTC 1 cut(s) 1257
AspLEI GCGC 2 cut(s) 251, 1126
AspS9I GGNCC 5 cut(s) 7, 226, 227, 505, 901
AvaII GGWCC 3 cut(s) 7, 505, 901
AxyI CCTNAGG 1 cut(s) 2085
BaeGI GKGCMC 1 cut(s) 230
BalI TGGCCA 2 cut(s) 689, 1424
BanII GRGCYC 4 cut(s) 230, 320, 2094, 2370
BbsI GAAGAC 1 cut(s) 2310
Bbv12I GWGCWC 4 cut(s) 320, 2094, 2354, 2370
BbvI GCAGC 5 cut(s) 424, 656, 843, 1354, 1376
BccI CCATC 3 cut(s) 148, 1082, 1685
BceAI ACGGC 1 cut(s) 22
BciT130I CCWGG 3 cut(s) 663, 905, 1082
BclI TGATCA 2 cut(s) 75, 1596
BcoDI GTCTC 9 cut(s) 368, 395, 464, 1241, 1409, 1481, 1608, 2338, 2417
BfaI CTAG 8 cut(s) 1110, 1574, 1724, 1742, 1901, 2355, 2399, 2501
BfmI CTRYAG 2 cut(s) 15, 1510
BfoI RGCGCY 1 cut(s) 1127
BfrI CTTAAG 1 cut(s) 623
BfuAI ACCTGC 2 cut(s) 8, 1849
BglII AGATCT 1 cut(s) 1962
BisI GCNGC 7 cut(s) 15, 413, 670, 857, 1365, 1368, 2195
BlpI GCTNAGC 1 cut(s) 2318
BlsI GCNGC 7 cut(s) 16, 414, 671, 858, 1366, 1369, 2196
Bme1390I CCNGG 3 cut(s) 663, 905, 1082
Bme18I GGWCC 3 cut(s) 7, 505, 901
BmgT120I GGNCC 5 cut(s) 7, 226, 227, 505, 901
BmiI GGNNCC 3 cut(s) 228, 2442, 2510
BmrFI CCNGG 3 cut(s) 663, 905, 1082
BmsI GCATC 5 cut(s) 1039, 1085, 1273, 1657, 1942
BoxI GACNNNNGTC 1 cut(s) 503
BpiI GAAGAC 1 cut(s) 2310
BpmI CTGGAG 1 cut(s) 684
Bpu10I CCTNAGC 2 cut(s) 231, 2198
Bpu1102I GCTNAGC 1 cut(s) 2318
BpuEI CTTGAG 1 cut(s) 2142
BsaI GGTCTC 3 cut(s) 368, 464, 1409
BsaJI CCNNGG 6 cut(s) 378, 474, 1080, 1081, 1330, 1609
BsaWI WCCGGW 1 cut(s) 2453
Bsc4I CCNNNNNNNGG 4 cut(s) 1010, 1072, 1133, 1459
Bse1I ACTGG 2 cut(s) 1872, 2178
Bse21I CCTNAGG 1 cut(s) 2085
Bse3DI GCAATG 4 cut(s) 353, 1059, 2027, 2071
BseAI TCCGGA 1 cut(s) 2453
BseBI CCWGG 3 cut(s) 663, 905, 1082
BseDI CCNNGG 6 cut(s) 378, 474, 1080, 1081, 1330, 1609
BseLI CCNNNNNNNGG 4 cut(s) 1010, 1072, 1133, 1459
BseMI GCAATG 4 cut(s) 353, 1059, 2027, 2071
BseMII CTCAG 9 cut(s) 164, 222, 1029, 1236, 1558, 2076, 2111, 2247, 2309
BseNI ACTGG 2 cut(s) 1872, 2178
BseRI GAGGAG 2 cut(s) 1672, 2102
BseSI GKGCMC 1 cut(s) 230
BseXI GCAGC 5 cut(s) 424, 656, 843, 1354, 1376
BsgI GTGCAG 2 cut(s) 431, 1999
Bsh1236I CGCG 1 cut(s) 1176
BshFI GGCC 3 cut(s) 228, 689, 1424
BsiHKAI GWGCWC 4 cut(s) 320, 2094, 2354, 2370
BsiSI CCGG 2 cut(s) 508, 2454
BslFI GGGAC 1 cut(s) 511
BslI CCNNNNNNNGG 4 cut(s) 1010, 1072, 1133, 1459
BsmAI GTCTC 9 cut(s) 368, 395, 464, 1241, 1409, 1481, 1608, 2338, 2417
BsmBI CGTCTC 1 cut(s) 395
BsmFI GGGAC 1 cut(s) 511
BsnI GGCC 3 cut(s) 228, 689, 1424
Bso31I GGTCTC 3 cut(s) 368, 464, 1409
Bsp120I GGGCCC 1 cut(s) 226
Bsp1286I GDGCHC 5 cut(s) 230, 320, 2094, 2354, 2370
Bsp13I TCCGGA 1 cut(s) 2453
Bsp1407I TGTACA 1 cut(s) 784
Bsp143I GATC 8 cut(s) 75, 178, 992, 1465, 1596, 1962, 2334, 2386
Bsp1720I GCTNAGC 1 cut(s) 2318
BspACI CCGC 7 cut(s) 5, 434, 511, 672, 1066, 1176, 2194
BspANI GGCC 3 cut(s) 228, 689, 1424
BspCNI CTCAG 9 cut(s) 163, 223, 1028, 1237, 1557, 2077, 2112, 2246, 2310
BspEI TCCGGA 1 cut(s) 2453
BspFNI CGCG 1 cut(s) 1176
BspLI GGNNCC 3 cut(s) 228, 2442, 2510
BspMAI CTGCAG 1 cut(s) 19
BspMI ACCTGC 2 cut(s) 8, 1849
BspPI GGATC 2 cut(s) 186, 2394
BspQI GCTCTTC 1 cut(s) 102
BspTI CTTAAG 1 cut(s) 623
BspTNI GGTCTC 3 cut(s) 368, 464, 1409
BsrDI GCAATG 4 cut(s) 353, 1059, 2027, 2071
BsrGI TGTACA 1 cut(s) 784
BsrI ACTGG 2 cut(s) 1872, 2178
BssECI CCNNGG 6 cut(s) 378, 474, 1080, 1081, 1330, 1609
BssMI GATC 8 cut(s) 75, 178, 992, 1465, 1596, 1962, 2334, 2386
BssT1I CCWWGG 3 cut(s) 378, 474, 1330
Bst2UI CCWGG 3 cut(s) 663, 905, 1082
Bst6I CTCTTC 5 cut(s) 102, 327, 1883, 1943, 2415
BstAFI CTTAAG 1 cut(s) 623
BstAPI GCANNNNNTGC 1 cut(s) 23
BstAUI TGTACA 1 cut(s) 784
BstC8I GCNNGC 1 cut(s) 1554
BstDSI CCRYGG 1 cut(s) 1609
BstFNI CGCG 1 cut(s) 1176
BstH2I RGCGCY 1 cut(s) 1127
BstHHI GCGC 2 cut(s) 251, 1126
BstKTI GATC 8 cut(s) 78, 181, 995, 1468, 1599, 1965, 2337, 2389
BstMAI GTCTC 9 cut(s) 368, 395, 464, 1241, 1409, 1481, 1608, 2338, 2417
BstMBI GATC 8 cut(s) 75, 178, 992, 1465, 1596, 1962, 2334, 2386
BstMWI GCNNNNNNNGC 4 cut(s) 11, 23, 1549, 2089
BstNI CCWGG 3 cut(s) 663, 905, 1082
BstPAI GACNNNNGTC 1 cut(s) 503
BstSCI CCNGG 3 cut(s) 661, 903, 1080
BstSFI CTRYAG 2 cut(s) 15, 1510
BstSLI GKGCMC 1 cut(s) 230
BstUI CGCG 1 cut(s) 1176
BstV1I GCAGC 5 cut(s) 424, 656, 843, 1354, 1376
BstV2I GAAGAC 1 cut(s) 2310
BstX2I RGATCY 2 cut(s) 1962, 2386
BstYI RGATCY 2 cut(s) 1962, 2386
Bsu36I CCTNAGG 1 cut(s) 2085
BsuRI GGCC 3 cut(s) 228, 689, 1424
BtgI CCRYGG 1 cut(s) 1609
BtgZI GCGATG 1 cut(s) 2430
BtsIMutI CAGTG 6 cut(s) 140, 750, 1034, 1497, 1519, 2354
BveI ACCTGC 2 cut(s) 8, 1849
Cac8I GCNNGC 1 cut(s) 1554
CaiI CAGNNNCTG 2 cut(s) 331, 1367
CfoI GCGC 2 cut(s) 251, 1126
Cfr13I GGNCC 5 cut(s) 7, 226, 227, 505, 901
Csp6I GTAC 7 cut(s) 212, 533, 557, 785, 810, 893, 2208
CspCI CAANNNNNGTGG 2 cut(s) 1137, 1172
CviAII CATG 6 cut(s) 1215, 1535, 1829, 1975, 2229, 2341
CviQI GTAC 7 cut(s) 212, 533, 557, 785, 810, 893, 2208
DpnI GATC 8 cut(s) 77, 180, 994, 1467, 1598, 1964, 2336, 2388
DpnII GATC 8 cut(s) 75, 178, 992, 1465, 1596, 1962, 2334, 2386
DraI TTTAAA 2 cut(s) 208, 1956
EaeI YGGCCR 2 cut(s) 687, 1422
Eam1104I CTCTTC 5 cut(s) 102, 327, 1883, 1943, 2415
EarI CTCTTC 5 cut(s) 102, 327, 1883, 1943, 2415
EciI GGCGGA 3 cut(s) 20, 526, 1055
Ecl136II GAGCTC 3 cut(s) 318, 2092, 2368
Eco130I CCWWGG 3 cut(s) 378, 474, 1330
Eco24I GRGCYC 4 cut(s) 230, 320, 2094, 2370
Eco31I GGTCTC 3 cut(s) 368, 464, 1409
Eco32I GATATC 1 cut(s) 1139
Eco47I GGWCC 3 cut(s) 7, 505, 901
Eco53kI GAGCTC 3 cut(s) 318, 2092, 2368
Eco57I CTGAAG 8 cut(s) 134, 308, 1272, 1826, 1907, 2266, 2322, 2375
Eco81I CCTNAGG 1 cut(s) 2085
EcoICRI GAGCTC 3 cut(s) 318, 2092, 2368
EcoO109I RGGNCCY 2 cut(s) 226, 227
EcoRI GAATTC 3 cut(s) 437, 1356, 2492
EcoRII CCWGG 3 cut(s) 661, 903, 1080
EcoRV GATATC 1 cut(s) 1139
EcoT14I CCWWGG 3 cut(s) 378, 474, 1330
EcoT22I ATGCAT 1 cut(s) 2029
EcoT38I GRGCYC 4 cut(s) 230, 320, 2094, 2370
ErhI CCWWGG 3 cut(s) 378, 474, 1330
Esp3I CGTCTC 1 cut(s) 395
FaeI CATG 6 cut(s) 1218, 1538, 1832, 1978, 2232, 2344
FalI AAGNNNNNCTT 8 cut(s) 362, 394, 458, 490, 755, 787, 1245, 1277
FaqI GGGAC 1 cut(s) 511
FatI CATG 6 cut(s) 1214, 1534, 1828, 1974, 2228, 2340
FauI CCCGC 1 cut(s) 665
FbaI TGATCA 2 cut(s) 75, 1596
FblI GTMKAC 1 cut(s) 1560
Fnu4HI GCNGC 7 cut(s) 15, 413, 670, 857, 1365, 1368, 2195
FriOI GRGCYC 4 cut(s) 230, 320, 2094, 2370
Fsp4HI GCNGC 7 cut(s) 15, 413, 670, 857, 1365, 1368, 2195
FspBI CTAG 8 cut(s) 1110, 1574, 1724, 1742, 1901, 2355, 2399, 2501
GlaI GCGC 2 cut(s) 250, 1125
GluI GCNGC 7 cut(s) 15, 413, 670, 857, 1365, 1368, 2195
GsuI CTGGAG 1 cut(s) 684
HaeII RGCGCY 1 cut(s) 1127
HaeIII GGCC 3 cut(s) 228, 689, 1424
HapII CCGG 2 cut(s) 508, 2454
HhaI GCGC 2 cut(s) 251, 1126
Hin1II CATG 6 cut(s) 1218, 1538, 1832, 1978, 2232, 2344
Hin6I GCGC 2 cut(s) 249, 1124
HinP1I GCGC 2 cut(s) 249, 1124
HincII GTYRAC 1 cut(s) 768
HindII GTYRAC 1 cut(s) 768
HindIII AAGCTT 4 cut(s) 129, 617, 1302, 1992
HinfI GANTC 9 cut(s) 136, 328, 923, 976, 1057, 1229, 1241, 1871, 1893
HpaII CCGG 2 cut(s) 508, 2454
Hpy166II GTNNAC 4 cut(s) 42, 768, 1517, 1561
Hpy8I GTNNAC 4 cut(s) 42, 768, 1517, 1561
HpyCH4IV ACGT 3 cut(s) 555, 1434, 1694
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 23, 1549, 2089
HpySE526I ACGT 3 cut(s) 555, 1434, 1694
Hsp92II CATG 6 cut(s) 1218, 1538, 1832, 1978, 2232, 2344
HspAI GCGC 2 cut(s) 249, 1124
Kpn2I TCCGGA 1 cut(s) 2453
Ksp22I TGATCA 2 cut(s) 75, 1596
Kzo9I GATC 8 cut(s) 75, 178, 992, 1465, 1596, 1962, 2334, 2386
LguI GCTCTTC 1 cut(s) 102
LmnI GCTCC 5 cut(s) 664, 2012, 2089, 2373, 2514
Lsp1109I GCAGC 5 cut(s) 424, 656, 843, 1354, 1376
LweI GCATC 5 cut(s) 1039, 1085, 1273, 1657, 1942
MaeI CTAG 8 cut(s) 1110, 1574, 1724, 1742, 1901, 2355, 2399, 2501
MaeII ACGT 3 cut(s) 555, 1434, 1694
MalI GATC 8 cut(s) 77, 180, 994, 1467, 1598, 1964, 2336, 2388
MboI GATC 8 cut(s) 75, 178, 992, 1465, 1596, 1962, 2334, 2386
MfeI CAATTG 1 cut(s) 2002
MflI RGATCY 2 cut(s) 1962, 2386
MhlI GDGCHC 5 cut(s) 230, 320, 2094, 2354, 2370
MlsI TGGCCA 2 cut(s) 689, 1424
MluNI TGGCCA 2 cut(s) 689, 1424
MlyI GAGTC 1 cut(s) 1051
MmeI TCCRAC 4 cut(s) 156, 364, 562, 1141
Mox20I TGGCCA 2 cut(s) 689, 1424
Mph1103I ATGCAT 1 cut(s) 2029
MroI TCCGGA 1 cut(s) 2453
MroXI GAANNNNTTC 1 cut(s) 1257
MscI TGGCCA 2 cut(s) 689, 1424
MslI CAYNNNNRTG 2 cut(s) 821, 1213
Msp20I TGGCCA 2 cut(s) 689, 1424
MspA1I CMGCKG 2 cut(s) 162, 1367
MspCI CTTAAG 1 cut(s) 623
MspI CCGG 2 cut(s) 508, 2454
MspR9I CCNGG 3 cut(s) 663, 905, 1082
MunI CAATTG 1 cut(s) 2002
MvaI CCWGG 3 cut(s) 663, 905, 1082
MvnI CGCG 1 cut(s) 1176
MwoI GCNNNNNNNGC 4 cut(s) 11, 23, 1549, 2089
NdeII GATC 8 cut(s) 75, 178, 992, 1465, 1596, 1962, 2334, 2386
NlaIII CATG 6 cut(s) 1218, 1538, 1832, 1978, 2232, 2344
NlaIV GGNNCC 3 cut(s) 228, 2442, 2510
NmuCI GTSAC 7 cut(s) 551, 748, 982, 1032, 1474, 1525, 1819
NsiI ATGCAT 1 cut(s) 2029
PaqCI CACCTGC 1 cut(s) 8
PasI CCCWGGG 1 cut(s) 1081
PciSI GCTCTTC 1 cut(s) 102
PdmI GAANNNNTTC 1 cut(s) 1257
PfeI GAWTC 8 cut(s) 136, 328, 923, 976, 1229, 1241, 1871, 1893
PfoI TCCNGGA 2 cut(s) 661, 903
PkrI GCNGC 7 cut(s) 16, 414, 671, 858, 1366, 1369, 2196
PleI GAGTC 1 cut(s) 1051
PpsI GAGTC 1 cut(s) 1051
PshAI GACNNNNGTC 1 cut(s) 503
Psp124BI GAGCTC 3 cut(s) 320, 2094, 2370
Psp1406I AACGTT 1 cut(s) 1694
Psp6I CCWGG 3 cut(s) 661, 903, 1080
PspGI CCWGG 3 cut(s) 661, 903, 1080
PspN4I GGNNCC 3 cut(s) 228, 2442, 2510
PspOMI GGGCCC 1 cut(s) 226
PspPI GGNCC 5 cut(s) 7, 226, 227, 505, 901
PstI CTGCAG 1 cut(s) 19
PstNI CAGNNNCTG 2 cut(s) 331, 1367
PsuI RGATCY 2 cut(s) 1962, 2386
PvuII CAGCTG 2 cut(s) 162, 1367
RsaI GTAC 7 cut(s) 213, 534, 558, 786, 811, 894, 2209
RsaNI GTAC 7 cut(s) 212, 533, 557, 785, 810, 893, 2208
RseI CAYNNNNRTG 2 cut(s) 821, 1213
SacI GAGCTC 3 cut(s) 320, 2094, 2370
SapI GCTCTTC 1 cut(s) 102
SatI GCNGC 7 cut(s) 15, 413, 670, 857, 1365, 1368, 2195
Sau3AI GATC 8 cut(s) 75, 178, 992, 1465, 1596, 1962, 2334, 2386
Sau96I GGNCC 5 cut(s) 7, 226, 227, 505, 901
SchI GAGTC 1 cut(s) 1051
ScrFI CCNGG 3 cut(s) 663, 905, 1082
SduI GDGCHC 5 cut(s) 230, 320, 2094, 2354, 2370
SfaNI GCATC 5 cut(s) 1039, 1085, 1273, 1657, 1942
SfcI CTRYAG 2 cut(s) 15, 1510
SinI GGWCC 3 cut(s) 7, 505, 901
SmiMI CAYNNNNRTG 2 cut(s) 821, 1213
SmlI CTYRAG 2 cut(s) 623, 2157
SmoI CTYRAG 2 cut(s) 623, 2157
SsiI CCGC 7 cut(s) 5, 434, 511, 672, 1066, 1176, 2194
SspI AATATT 2 cut(s) 2053, 2525
SspMI CTAG 8 cut(s) 1110, 1574, 1724, 1742, 1901, 2355, 2399, 2501
SstI GAGCTC 3 cut(s) 320, 2094, 2370
StyD4I CCNGG 3 cut(s) 661, 903, 1080
StyI CCWWGG 3 cut(s) 378, 474, 1330
TaiI ACGT 3 cut(s) 558, 1437, 1697
TaqI TCGA 3 cut(s) 86, 1407, 1700
TatI WGTACW 3 cut(s) 211, 784, 892
TauI GCSGC 1 cut(s) 2197
TfiI GAWTC 8 cut(s) 136, 328, 923, 976, 1229, 1241, 1871, 1893
TscAI CASTG 6 cut(s) 147, 757, 1041, 1497, 1519, 2354
TseFI GTSAC 7 cut(s) 551, 748, 982, 1032, 1474, 1525, 1819
TseI GCWGC 6 cut(s) 14, 412, 669, 856, 1364, 1367
Tsp45I GTSAC 7 cut(s) 551, 748, 982, 1032, 1474, 1525, 1819
TspGWI ACGGA 2 cut(s) 2225, 2481
TspRI CASTG 6 cut(s) 147, 757, 1041, 1497, 1519, 2354
Vha464I CTTAAG 1 cut(s) 623
VpaK11BI GGWCC 3 cut(s) 7, 505, 901
XbaI TCTAGA 1 cut(s) 2354
XcmI CCANNNNNNNNNTGG 1 cut(s) 1456
XmiI GTMKAC 1 cut(s) 1560
XmnI GAANNNNTTC 1 cut(s) 1257
XspI CTAG 8 cut(s) 1110, 1574, 1724, 1742, 1901, 2355, 2399, 2501
Zsp2I ATGCAT 1 cut(s) 2029
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.