Rh2DG178800

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0000039
Physical Location & Seq
Reverse (-)
15721051 .. 15727486
6436 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG178800.1

Sequence Viewer

Length: 3738 bp
ATGGTAATAGTGGGGGAGTTTGTTGGAGGGGCTGCTCTTGGAGCAGCATTCGGCACTCTCTTTGATGTGGTTAAGGAAGCGTTGGAGAAATCTGCCATGTTTAAACCCTTACTCAAGACCATTAAATCCACTCTTGATTCTTTGCAACCGCTGATCCAACAGATAGAAATGCGCAATGCTGAATTGAACCTCCCGAACGAGGAAATAGAATCCTGTAAGAAGCAGATGGAGGAAGGTGTTGAGCTCGTTCGAAAGCTATCCAAAGTTTCCAAGTGGAACTACAGGAAGCCTCGTTACACTGATCAACTTGTTCAGTTGGATGGATCCCTTAAGAGAATGTTGGATATAATGAAGGTGCAGGAAGCAAGGGATGTGAAGGAAACCTTGGTTTTGGTGCGGAGTATAGATACTGTGGTCAAGCGACTTGAAGGGAATGGTTTGGTACAGAGTCAATTTGAGATCAACGGTTGTTGTGCAGCGCCTGATGAACAACCTCCTCCAACGATTACAGTTGGATTGGATGCTCCATTGGAGGAACTAAAGAAGAATTTTCTCAAGGATGGGGTGTCGATGCTTGTACTGGCTGGTCCTGGAGGATGTGGGAAAACCACATTGGCAAAAGCCTTCTGTCAAGATCAGGAAGTCAAAGATATATTCAAGAAAAATATCTTCTTTGTTACTGTTTCCAAAACGCCCAACTTGAACCATATTGTTCGAGAACTGTGTCAACTTAAGGGCTTTCAGGTGCCTTTTTTCAATACCGAAGTAACTGCAGTTAGCTGGCTGGAAACATTTCTAAGGGAAACTGTTCAACATCCTCTACTGTTGGTCCTAGATGGTGTTTGGTCTGGATCAGAATCCCTTCTTAACAAGTTTGCGATCAAAATGTCAAATTACAAGATTTTGGTGACATCAAGATTTGCATTTCCGAGATTTGGTTCTCCATATTATTTAGCATCACTGACACATGAAGATGCAATGACTCTTTTTCGTCATTCCGCATCCTTGGGAGATAGGAGCTCTTATATTCCAGAAGATCTTCCAAGAAAGATAGTGGAGCGCTGTAAGGGATTTCCTCCTGCCATTACAATGGTAGGAAGGTCACTTTGCGGGCAGCCTGTTGAGATTTGGCAAAATAGATTGATGGAATGGTCAAGAGGTTCTTCTATTCTTGATTCTGAGAGTGATTTGCTTGACTACCTCAAAAACAACTTAGCTTTCTTGGACAAAGGAGATATGACTATCGTCAAGGAATGTTTCATAGACCTTGGCTCGTTTCCTGAAGACCAGAGAATCCCTGTTGCGGGCCTTATTGATATGTGGGCAGAACTATATGAGCTAGATGAAGACTGCATGTCCATTGCAAATCTCTATGAACTTGCTTCCCAGGGTCTGGCAAATCTTGTATGCACAAGAAAAGATAACTGGGAGGTGGATAGATACTTCAGCGAACACTTTGTCACCCAACACTATATGCTTAGAGAGCTAGCTATCCATCAGACTAGTCAGGAACCAATAGCACAGAGAAAAAAATTGATTATTGACATATGTGGAGACAATCTTCCCAGATGGTGGACAGAACAGAAGTTTCAACCCGTTAAAGCACGCTTGTTATCTATCTCAACTGATGGAGTGTTCTCAACAAAATGGCACAACATGCAACTACCACAGGCTGAGGTTTTAGTGCTGAACTTTCAGACAAAGAACTATGCCTTACCCGAATTTGTGGAGAAAATGGATAAACTGAAGGTTCTAATAGTCACAAATTATGGTTTCTTACCTGCTGAATTAAGCAATTTTCAACTCTTGGGGTCTTCATCAAATCTAAAGAGAATCAGATTAGAGCGGATTTCAATTCCTTCTCTAATTAATGACTCTGTACAATTGAAGAGTCTGCAGAAGATATCTTTATATATGTGTAACATTGGCCAAGCTTTTAGCAATTGCTCCATCCAAATTACATATGCATTTCCAAATCTAGTAGAAATGAACATTGATTACTGCAATGATTTGTTGGATTTACCTTACAACCTTTGTGATCTTATTCATCTAAAGAAGCTCAGTATCACAAACTGTCATAAGCTATATGCCTTGCCCGAAGAAATTGGAAAGCTGGTCAATCTACAGGTCTTGAGGCTGAGGTCTTGTATACACTTGTTAGAGTTGCCAAATTCGATCAGAAGCCTCAAAATGCTATACTTTCTGGACATATCTGATTGCTACAGCATGAAGGAATTGCCTGAAGATATTGGAGAGTTGTGCAGTTTGAAAAAGCTCAATATGAGGCAGTGTTCAAAATTGCAGGAGTTGCCCCCATCAGTTTTGTGGCTCAAGTTGTTACAGGAAGTGGTATGTGATGAACATACAGAAAAATTATGGGAACCTTTCATACCCTATCTCAGAAAGATACACATAAAGGTGGCCAAACAGAATTGGCACCCCTGCTCGGATTGGACTAATCTTGTAGTGACAAGGATGGAAAAGACAGAGGTAGTGGACAACATGGTTGAATCGCAGATAAACACAGTTCAGGCTGACATACAAGCAGAGTTTAAGGGTGAGATAAAGGTGGCCGGAGATAATTTGTTCCCCACCCAGAGTCAATCTAATCTTGTAGTCACAAGGGTGGAGAAGAGAGAGGTGATGGATAAACCAGTTAAATCACATATTGAGACCGTTAAGACAGAGTTACAAGCAGAGTTTAAGGCCGAGATGAAAACACTCAGAGACGAGTTTACAAATAAACTTGATGACATTACGGATTTACTTGTCCACCTCATACAAGGAAAGAATGAGAAGGAATGGGAAGGCAAGGACATTCCAGAACAAATAACAAGTGTTTTCGGAGACTATGAGAGTGGGGCTGCCAAATCAGGTTTCCGTATGCATAGAAACTCAAAGAAGGTAACACACCAACTTCAAAAGGTCAATCAGCTTAGCCCTCTCCCGTCTGAGGAACAGATAGTGAGGCAGTTTAGTGCATGGATCAGTAATCCAAACGCCAGCCGGTGTAACTTGGAAATCTTTGATGCACCTAAAGAGTGGTTTATAGACTTGATGACCTCGGGCACATGGTTGAGTGACTCGCATGTAGATGTGTTCTTGTATGCTGTTCGTAAGAGAGCAAATCTTCAGTCCCAATCTTCCTCTCAGTGTTGCACCATCTTGGATACTGTGTTTTGGAGTTGGATGAATGGGCGATGGGAGAATTTTCAGAACAACTCCAAGTCCTATAAGTGGGATTCAGACCTTAGGGACTACCCGCTTGGCAAGTCGCCTAAATATGCTCGAAGATGGAGTGAAGTTGATCATTTGTACCTTCCGATCAACATCAACAACCAGCATTGGGTAGCTGTATGCGCGGACATAATACGAAGAAAGTTGACGGTATACAATTCTATGCGGTCTGCAACTCAAGATGAGTTCATTTCTAACATATTGAAGCCCATGGCGACAATGTTGCCATCGCTACTTGTCCAGTCTGGCTTTTACGAACACAGGCCTGAGTTGTCCCCTCTGACTACTCCGTTTAAGGTGGTACATTTAGCAGACAACATTCCGCAACAGATTGTCAGTGGCGATTGTGGGGTGTTCATGCTCAATTACATAGAGTACTTGAGCTACGGACGACCATTGGGTTTTGTACAAGCAGACATACAACTATTTAGGGAGAAAATGGCATTGGAGATGTTTGCCTTGACAATGATGGGGTGTTCTCAACAAAATAGAAGCTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

1245

Amino Acids

142.65

Weight (kDa)

6.17

Isoelectric Point (pI)

45.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 6 - 138 3.3e-32 Arabidopsis broad-spectrum mildew resistance protein RPW8
NB-ARC PF00931 175 - 307 3e-12 NB-ARC domain
LRR_14 PF23598 657 - 740 3.8e-09 Leucine-rich repeat region
Peptidase_C48 PF02902 1025 - 1231 5.1e-33 Ulp1 protease family, C-terminal catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000039)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66890 AT5G66900 AT5G66900 AT5G66900 AT5G66910
fragaria_vesca FvH4_1g15090 FvH4_1g15120 FvH4_1g15130 FvH4_1g15140 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15170 FvH4_1g15171 FvH4_1g15171 FvH4_1g15200 FvH4_1g15200 FvH4_1g15200 FvH4_1g15210 FvH4_1g15210 FvH4_1g15210 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15230 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15500 FvH4_1g15500 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_2g19760 FvH4_3g12820 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_6g18970 FvH4_6g42370 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g50100 FvH4_6g50100 FvH4_6g50110 FvH4_6g50130 FvH4_6g50140 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50330
malus_domestica MD00G1053700.v1.1 MD02G1164300.v1.1 MD02G1164400.v1.1 MD02G1164700.v1.1 MD02G1164800.v1.1 MD02G1165300.v1.1 MD02G1165400.v1.1 MD02G1165500.v1.1 MD02G1165600.v1.1 MD02G1165900.v1.1 MD02G1166000.v1.1 MD02G1166100.v1.1 MD02G1166300.v1.1 MD08G1084900.v1.1 MD15G1263100.v1.1 MD15G1263300.v1.1 MD15G1276900.v1.1 MD15G1277200.v1.1 MD15G1277400.v1.1 MD15G1277700.v1.1 MD15G1277800.v1.1 MD15G1278000.v1.1 MD15G1278100.v1.1 MD15G1278400.v1.1 MD15G1278600.v1.1
prunus_persica Prupe.7G138500_v2.0.a1 Prupe.7G138500_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138700_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138900_v2.0.a1 Prupe.7G139100_v2.0.a1 Prupe.7G139200_v2.0.a1 Prupe.7G139500_v2.0.a1 Prupe.7G139600_v2.0.a1 Prupe.7G139700_v2.0.a1 Prupe.7G139800_v2.0.a1
pyrus_communis pycom02g13150 pycom02g13160 pycom02g13190 pycom02g13200 pycom02g13220 pycom02g13230 pycom02g13250 pycom02g13260 pycom02g13270 pycom02g13300 pycom02g13310 pycom02g13330 pycom15g23980 pycom15g23990 pycom15g24030 pycom15g24040 pycom15g24050 pycom15g24070 pycom15g24090 pycom15g24160
rosa_chinensis RchiOBHm_Chr1g0326431 RchiOBHm_Chr1g0326451 RchiOBHm_Chr2g0104121 RchiOBHm_Chr2g0104151 RchiOBHm_Chr2g0104171 RchiOBHm_Chr2g0104181 RchiOBHm_Chr2g0104191 RchiOBHm_Chr2g0104201 RchiOBHm_Chr2g0104211 RchiOBHm_Chr2g0104241 RchiOBHm_Chr2g0104261 RchiOBHm_Chr2g0104281 RchiOBHm_Chr2g0104311 RchiOBHm_Chr2g0104331 RchiOBHm_Chr2g0104361 RchiOBHm_Chr2g0104381 RchiOBHm_Chr2g0104391 RchiOBHm_Chr2g0104431 RchiOBHm_Chr2g0104441 RchiOBHm_Chr2g0104541 RchiOBHm_Chr2g0104611 RchiOBHm_Chr2g0104741 RchiOBHm_Chr2g0104761 RchiOBHm_Chr2g0158291 RchiOBHm_Chr2g0170371 RchiOBHm_Chr2g0170631 RchiOBHm_Chr3g0473971 RchiOBHm_Chr3g0480491 RchiOBHm_Chr3g0480541 RchiOBHm_Chr3g0480561 RchiOBHm_Chr3g0485281 RchiOBHm_Chr3g0485291 RchiOBHm_Chr4g0411541 RchiOBHm_Chr6g0285471
rosa_laevigata RLG00000008347 RLG00000012637 RLG00000016961 RLG00000017293 RLG00000017305 RLG00000017308 RLG00000017312 RLG00000017314 RLG00000017317 RLG00000017318 RLG00000017319 RLG00000017321 RLG00000017322 RLG00000017323 RLG00000017324 RLG00000017325 RLG00000017327 RLG00000017328 RLG00000017329 RLG00000017330 RLG00000017334 RLG00000020833 RLG00000021073 RLG00000021950 RLG00000021971 RLG00000023154 RLG00000023157 RLG00000023467 RLG00000023468 RLG00000023525 RLG00000023963 RLG00000033265 RLG00000033267 RLG00000033268
rosa_multiflora Rmu_co8053082.1_g000001 Rmu_co8210358.1_g000001 Rmu_co8331661.1_g000001 Rmu_co8477427.1_g000002 Rmu_co8483217.1_g000001 Rmu_sc0000284.1_g000004 Rmu_sc0000676.1_g000017 Rmu_sc0000676.1_g000019 Rmu_sc0000676.1_g000021 Rmu_sc0002205.1_g000038 Rmu_sc0002205.1_g000041 Rmu_sc0002316.1_g000010 Rmu_sc0002316.1_g000012 Rmu_sc0002316.1_g000040 Rmu_sc0002316.1_g000071 Rmu_sc0002316.1_g000072 Rmu_sc0002316.1_g000095 Rmu_sc0002316.1_g000102 Rmu_sc0002414.1_g000018 Rmu_sc0002576.1_g000002 Rmu_sc0002576.1_g000003 Rmu_sc0002687.1_g000010 Rmu_sc0002687.1_g000017 Rmu_sc0003660.1_g000001 Rmu_sc0003787.1_g000014 Rmu_sc0003787.1_g000020 Rmu_sc0003787.1_g000024 Rmu_sc0003787.1_g000029 Rmu_sc0004059.1_g000026 Rmu_sc0005137.1_g000021 Rmu_sc0006550.1_g000001 Rmu_sc0006561.1_g000025 Rmu_sc0006561.1_g000033 Rmu_sc0007704.1_g000005 Rmu_sc0008904.1_g000005 Rmu_sc0010270.1_g000002 Rmu_sc0010307.1_g000001 Rmu_sc0011601.1_g000013 Rmu_sc0012694.1_g000005 Rmu_sc0013101.1_g000008 Rmu_sc0019029.1_g000001 Rmu_sc0019728.1_g000001 Rmu_sc0025829.1_g000001 Rmu_sc0025829.1_g000002 Rmu_ssc0000257.1_g000007 Rmu_ssc0000257.1_g000008
rosa_roxburghii Rroxscaffold_1G00021710 Rroxscaffold_1G00049350 Rroxscaffold_1G00049360 Rroxscaffold_2G00081160 Rroxscaffold_2G00081360 Rroxscaffold_2G00081390 Rroxscaffold_2G00091540 Rroxscaffold_2G00138790 Rroxscaffold_2G00138800 Rroxscaffold_2G00138920 Rroxscaffold_2G00139000 Rroxscaffold_2G00139010 Rroxscaffold_2G00139020 Rroxscaffold_2G00139030 Rroxscaffold_2G00139040 Rroxscaffold_2G00139050 Rroxscaffold_2G00139060 Rroxscaffold_2G00139070 Rroxscaffold_2G00139090 Rroxscaffold_2G00139100 Rroxscaffold_3G00257470 Rroxscaffold_4G00318040 Rroxscaffold_4G00323740 Rroxscaffold_4G00323750 Rroxscaffold_5G00356140 Rroxscaffold_6G00401280 Rroxscaffold_6G00401290 Rroxscaffold_7G00183120
rosa_rugosa Rorug01G0060500 Rorug01G0060900 Rorug02G0119500 Rorug02G0119600 Rorug02G0119700 Rorug02G0119700 Rorug02G0119700 Rorug02G0119800.1 Rorug02G0119900.1 Rorug02G0120000 Rorug02G0120100 Rorug02G0120200 Rorug02G0120300 Rorug02G0120400 Rorug02G0120500 Rorug02G0120700 Rorug02G0120700 Rorug02G0122300 Rorug02G0122300 Rorug02G0122400.1 Rorug02G0466700 Rorug02G0466700 Rorug02G0466800 Rorug02G0548600 Rorug02G0549900 Rorug02G0550000 Rorug03G0184200 Rorug04G0107900 Rorug05G0121500 Rorug06G0174800
rosa_samantha Rh1AG198400 Rh1CG074700 Rh1CG075000 Rh1CG183700 Rh2AG169700 Rh2AG169800 Rh2AG169900 Rh2AG170000 Rh2AG170300 Rh2AG170400 Rh2AG171000 Rh2AG171100 Rh2AG171600 Rh2AG172400 Rh2AG172500 Rh2AG532900 Rh2AG620000 Rh2AG622500 Rh2AG622800 Rh2AG623000 Rh2BG176200 Rh2BG176300 Rh2BG176400 Rh2BG176500 Rh2BG176600 Rh2BG176900 Rh2BG177000 Rh2BG177100 Rh2BG177200 Rh2BG177300 Rh2BG177600 Rh2BG177700 Rh2BG177900 Rh2BG178000 Rh2BG178200 Rh2BG178300 Rh2BG178500 Rh2BG179000 Rh2BG180000 Rh2BG180100 Rh2BG259200 Rh2BG546700 Rh2BG630800 Rh2BG633500 Rh2CG176500 Rh2CG177500 Rh2CG177700 Rh2DG174400 Rh2DG174600 Rh2DG174900 Rh2DG175100 Rh2DG175200 Rh2DG175300 Rh2DG175600 Rh2DG175700 Rh2DG175800 Rh2DG175900 Rh2DG176000 Rh2DG176300 Rh2DG176400 Rh2DG176500 Rh2DG176600 Rh2DG176900 Rh2DG177100 Rh2DG177200 Rh2DG177800 Rh2DG177900 Rh2DG178700 Rh2DG178800 Rh2DG555700 Rh2DG643700 Rh2DG646300 Rh3AG235600 Rh3AG235900 Rh3AG236200 Rh3AG266700 Rh3AG266900 Rh3BG216200 Rh3BG269100 Rh3CG212700 Rh3CG265800 Rh4AG168900 Rh4BG165500 Rh4CG180300 Rh5BG261500 Rh6AG286300 Rh6BG287500 Rh6CG288600 Rh7AG022600
rosa_wichuraiana Rw0G008240 Rw0G008250 Rw0G013190 Rw0G013200 Rw2G013230 Rw2G013240 Rw2G013270 Rw2G013280 Rw2G013290 Rw2G013300 Rw2G013330 Rw2G013340 Rw2G013350 Rw2G013360 Rw2G013370 Rw2G013380 Rw2G013410 Rw2G013570 Rw2G044120 Rw2G051370 Rw2G051590 Rw3G017160 Rw3G021150 Rw3G021160 Rw3G023730 Rw3G023740 Rw4G014030 Rw6G024590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 173
Acc36I ACCTGC 1 cut(s) 1789
AccB1I GGYRCC 2 cut(s) 745, 2436
AccB7I CCANNNNNTGG 2 cut(s) 1393, 1572
AccBSI CCGCTC 1 cut(s) 1846
AccI GTMKAC 2 cut(s) 2149, 3390
AccII CGCG 1 cut(s) 3362
AclWI GGATC 5 cut(s) 148, 318, 331, 859, 2993
AcoI YGGCCR 3 cut(s) 1927, 2421, 2571
AcsI RAATTY 4 cut(s) 547, 1721, 2170, 3208
AcuI CTGAAG 5 cut(s) 1302, 1429, 1766, 2262, 3116
AfaI GTAC 7 cut(s) 444, 579, 1881, 3317, 3540, 3614, 3645
AfeI AGCGCT 1 cut(s) 1061
AflII CTTAAG 2 cut(s) 329, 731
AhdI GACNNNNNGTC 1 cut(s) 1354
AhlI ACTAGT 1 cut(s) 1502
AjnI CCWGG 2 cut(s) 589, 1386
AjuI GAANNNNNNNTTGG 8 cut(s) 368, 400, 596, 628, 2861, 2893, 3665, 3697
AleI CACNNNNGTG 1 cut(s) 2624
Alw21I GWGCWC 2 cut(s) 246, 1022
Alw26I GTCTC 4 cut(s) 1548, 2666, 2721, 2841
AlwI GGATC 5 cut(s) 148, 318, 331, 859, 2993
AlwNI CAGNNNCTG 2 cut(s) 482, 1393
Ama87I CYCGRG 1 cut(s) 3064
Aor51HI AGCGCT 1 cut(s) 1061
AoxI GGCC 6 cut(s) 1306, 1927, 2421, 2571, 2706, 3500
ApeKI GCWGC 6 cut(s) 32, 44, 476, 1114, 2864, 3732
ApoI RAATTY 4 cut(s) 547, 1721, 2170, 3208
ArsI GACNNNNNNTTYG 4 cut(s) 1443, 1475, 3119, 3151
AseI ATTAAT 1 cut(s) 1869
Asp700I GAANNNNTTC 4 cut(s) 792, 807, 861, 1038
AspLEI GCGC 4 cut(s) 174, 481, 1062, 3362
AspS9I GGNCC 3 cut(s) 587, 829, 1306
AsuHPI GGTGA 4 cut(s) 919, 1453, 2570, 2653
AsuII TTCGAA 1 cut(s) 250
AsuNHI GCTAGC 1 cut(s) 1486
AvaI CYCGRG 1 cut(s) 3064
AvaII GGWCC 2 cut(s) 587, 829
AxyI CCTNAGG 1 cut(s) 3251
BaeGI GKGCMC 1 cut(s) 3071
BaeI ACNNNNGTAYC 2 cut(s) 399, 432
BalI TGGCCA 2 cut(s) 1929, 2423
BamHI GGATCC 1 cut(s) 323
BanI GGYRCC 2 cut(s) 745, 2436
BanII GRGCYC 2 cut(s) 246, 1022
BarI GAAGNNNNNNTAC 2 cut(s) 278, 310
BbsI GAAGAC 3 cut(s) 1290, 1353, 1806
Bbv12I GWGCWC 2 cut(s) 246, 1022
BbvCI CCTCAGC 2 cut(s) 1674, 2138
BbvI GCAGC 6 cut(s) 19, 56, 488, 1126, 2851, 3719
BcgI CGANNNNNNTGC 4 cut(s) 752, 786, 3442, 3476
BciT130I CCWGG 2 cut(s) 591, 1388
BciVI GTATCC 1 cut(s) 3163
BclI TGATCA 2 cut(s) 301, 3307
BcoDI GTCTC 4 cut(s) 1548, 2666, 2721, 2841
BcuI ACTAGT 1 cut(s) 1502
BfaI CTAG 5 cut(s) 833, 1340, 1487, 1503, 1979
BfmI CTRYAG 5 cut(s) 280, 771, 1895, 2123, 2221
BfoI RGCGCY 2 cut(s) 482, 1063
BfrI CTTAAG 2 cut(s) 329, 731
BfuAI ACCTGC 1 cut(s) 1789
BfuI GTATCC 1 cut(s) 3163
BglII AGATCT 1 cut(s) 1036
BisI GCNGC 6 cut(s) 33, 45, 477, 1115, 2865, 3733
BlpI GCTNAGC 1 cut(s) 2936
BlsI GCNGC 6 cut(s) 34, 46, 478, 1116, 2866, 3734
BmcAI AGTACT 1 cut(s) 3614
Bme1390I CCNGG 2 cut(s) 591, 1388
Bme18I GGWCC 2 cut(s) 587, 829
BmeRI GACNNNNNGTC 1 cut(s) 1354
BmeT110I CYCGRG 1 cut(s) 3064
BmgT120I GGNCC 3 cut(s) 587, 829, 1306
BmiI GGNNCC 5 cut(s) 325, 747, 1512, 2382, 2438
BmrFI CCNGG 2 cut(s) 591, 1388
BmrI ACTGGG 1 cut(s) 1435
BmsI GCATC 6 cut(s) 511, 561, 964, 965, 1010, 3019
BmtI GCTAGC 1 cut(s) 1490
BmuI ACTGGG 1 cut(s) 1435
BoxI GACNNNNGTC 1 cut(s) 1244
BpiI GAAGAC 3 cut(s) 1290, 1353, 1806
BpmI CTGGAG 1 cut(s) 612
Bpu10I CCTNAGC 2 cut(s) 1674, 2138
Bpu1102I GCTNAGC 1 cut(s) 2936
Bpu14I TTCGAA 1 cut(s) 250
BpuEI CTTGAG 6 cut(s) 98, 539, 2152, 2315, 3399, 3637
BsaBI GATNNNNATC 2 cut(s) 856, 3329
BsaI GGTCTC 1 cut(s) 2666
BsaJI CCNNGG 7 cut(s) 384, 1005, 1267, 1386, 1387, 3063, 3447
Bse118I RCCGGY 1 cut(s) 3006
Bse1I ACTGG 4 cut(s) 585, 1430, 2654, 3478
Bse21I CCTNAGG 1 cut(s) 3251
Bse3DI GCAATG 4 cut(s) 181, 984, 1359, 2011
Bse8I GATNNNNATC 2 cut(s) 856, 3329
BseBI CCWGG 2 cut(s) 591, 1388
BseDI CCNNGG 7 cut(s) 384, 1005, 1267, 1386, 1387, 3063, 3447
BseJI GATNNNNATC 2 cut(s) 856, 3329
BseMI GCAATG 4 cut(s) 181, 984, 1359, 2011
BseMII CTCAG 9 cut(s) 1170, 1665, 2074, 2129, 2413, 2737, 2943, 3164, 3495
BseNI ACTGG 4 cut(s) 585, 1430, 2654, 3478
BseRI GAGGAG 1 cut(s) 486
BseSI GKGCMC 1 cut(s) 3071
BseXI GCAGC 6 cut(s) 19, 56, 488, 1126, 2851, 3719
BsgI GTGCAG 3 cut(s) 377, 495, 2281
Bsh1236I CGCG 1 cut(s) 3362
BshFI GGCC 6 cut(s) 1308, 1929, 2423, 2573, 2708, 3502
BshNI GGYRCC 2 cut(s) 745, 2436
BsiHKAI GWGCWC 2 cut(s) 246, 1022
BsiHKCI CYCGRG 1 cut(s) 3064
BsiSI CCGG 2 cut(s) 2574, 3007
BslFI GGGAC 3 cut(s) 3121, 3269, 3496
BsmAI GTCTC 4 cut(s) 1548, 2666, 2721, 2841
BsmBI CGTCTC 1 cut(s) 2721
BsmFI GGGAC 3 cut(s) 3121, 3269, 3496
BsmI GAATGC 1 cut(s) 47
BsnI GGCC 6 cut(s) 1308, 1929, 2423, 2573, 2708, 3502
Bso31I GGTCTC 1 cut(s) 2666
BsoBI CYCGRG 1 cut(s) 3064
Bsp119I TTCGAA 1 cut(s) 250
Bsp1286I GDGCHC 3 cut(s) 246, 1022, 3071
Bsp1407I TGTACA 2 cut(s) 1879, 3643
Bsp1720I GCTNAGC 1 cut(s) 2936
Bsp19I CCATGG 1 cut(s) 3447
BspANI GGCC 6 cut(s) 1308, 1929, 2423, 2573, 2708, 3502
BspCNI CTCAG 9 cut(s) 1171, 1666, 2073, 2130, 2412, 2736, 2944, 3163, 3496
BspFNI CGCG 1 cut(s) 3362
BspLI GGNNCC 5 cut(s) 325, 747, 1512, 2382, 2438
BspMAI CTGCAG 2 cut(s) 775, 1899
BspMI ACCTGC 1 cut(s) 1789
BspOI GCTAGC 1 cut(s) 1490
BspPI GGATC 5 cut(s) 148, 318, 331, 859, 2993
BspT104I TTCGAA 1 cut(s) 250
BspT107I GGYRCC 2 cut(s) 745, 2436
BspTI CTTAAG 2 cut(s) 329, 731
BspTNI GGTCTC 1 cut(s) 2666
BsrBI CCGCTC 1 cut(s) 1846
BsrDI GCAATG 4 cut(s) 181, 984, 1359, 2011
BsrFI RCCGGY 1 cut(s) 3006
BsrGI TGTACA 2 cut(s) 1879, 3643
BsrI ACTGG 4 cut(s) 585, 1430, 2654, 3478
BssAI RCCGGY 1 cut(s) 3006
BssECI CCNNGG 7 cut(s) 384, 1005, 1267, 1386, 1387, 3063, 3447
BssNAI GTATAC 2 cut(s) 2150, 3391
BssT1I CCWWGG 4 cut(s) 384, 1005, 1267, 3447
Bst1107I GTATAC 2 cut(s) 2150, 3391
Bst2UI CCWGG 2 cut(s) 591, 1388
Bst6I CTCTTC 2 cut(s) 1883, 2627
BstAFI CTTAAG 2 cut(s) 329, 731
BstAPI GCANNNNNTGC 2 cut(s) 1657, 2308
BstAUI TGTACA 2 cut(s) 1879, 3643
BstBI TTCGAA 1 cut(s) 250
BstC8I GCNNGC 6 cut(s) 782, 1112, 1306, 1488, 1606, 3004
BstDSI CCRYGG 1 cut(s) 3447
BstFNI CGCG 1 cut(s) 3362
BstH2I RGCGCY 2 cut(s) 482, 1063
BstHHI GCGC 4 cut(s) 174, 481, 1062, 3362
BstMAI GTCTC 4 cut(s) 1548, 2666, 2721, 2841
BstMWI GCNNNNNNNGC 5 cut(s) 41, 1483, 1657, 2308, 3359
BstNI CCWGG 2 cut(s) 591, 1388
BstNSI RCATGY 3 cut(s) 1357, 1660, 3092
BstPAI GACNNNNGTC 1 cut(s) 1244
BstSCI CCNGG 2 cut(s) 589, 1386
BstSFI CTRYAG 5 cut(s) 280, 771, 1895, 2123, 2221
BstSLI GKGCMC 1 cut(s) 3071
BstUI CGCG 1 cut(s) 3362
BstV1I GCAGC 6 cut(s) 19, 56, 488, 1126, 2851, 3719
BstV2I GAAGAC 3 cut(s) 1290, 1353, 1806
BstX2I RGATCY 2 cut(s) 323, 1036
BstXI CCANNNNNNTGG 1 cut(s) 1090
BstYI RGATCY 2 cut(s) 323, 1036
BstZ17I GTATAC 2 cut(s) 2150, 3391
Bsu36I CCTNAGG 1 cut(s) 3251
BsuI GTATCC 1 cut(s) 3163
BsuRI GGCC 6 cut(s) 1308, 1929, 2423, 2573, 2708, 3502
BtgI CCRYGG 1 cut(s) 3447
BtgZI GCGATG 2 cut(s) 3214, 3450
BtsI GCAGTG 1 cut(s) 2294
BtsIMutI CAGTG 5 cut(s) 297, 959, 2294, 3158, 3580
BveI ACCTGC 1 cut(s) 1789
Cac8I GCNNGC 6 cut(s) 782, 1112, 1306, 1488, 1606, 3004
CaiI CAGNNNCTG 2 cut(s) 482, 1393
CfoI GCGC 4 cut(s) 174, 481, 1062, 3362
Cfr10I RCCGGY 1 cut(s) 3006
Cfr13I GGNCC 3 cut(s) 587, 829, 1306
Csp6I GTAC 7 cut(s) 443, 578, 1880, 3316, 3539, 3613, 3644
CviQI GTAC 7 cut(s) 443, 578, 1880, 3316, 3539, 3613, 3644
DraI TTTAAA 1 cut(s) 103
DriI GACNNNNNGTC 1 cut(s) 1354
EaeI YGGCCR 3 cut(s) 1927, 2421, 2571
Eam1104I CTCTTC 2 cut(s) 1883, 2627
Eam1105I GACNNNNNGTC 1 cut(s) 1354
EarI CTCTTC 2 cut(s) 1883, 2627
Ecl136II GAGCTC 2 cut(s) 244, 1020
Eco130I CCWWGG 4 cut(s) 384, 1005, 1267, 3447
Eco147I AGGCCT 1 cut(s) 3502
Eco24I GRGCYC 2 cut(s) 246, 1022
Eco31I GGTCTC 1 cut(s) 2666
Eco32I GATATC 1 cut(s) 1905
Eco47I GGWCC 2 cut(s) 587, 829
Eco47III AGCGCT 1 cut(s) 1061
Eco53kI GAGCTC 2 cut(s) 244, 1020
Eco57I CTGAAG 5 cut(s) 1302, 1429, 1766, 2262, 3116
Eco81I CCTNAGG 1 cut(s) 3251
Eco88I CYCGRG 1 cut(s) 3064
EcoICRI GAGCTC 2 cut(s) 244, 1020
EcoRII CCWGG 2 cut(s) 589, 1386
EcoRV GATATC 1 cut(s) 1905
EcoT14I CCWWGG 4 cut(s) 384, 1005, 1267, 3447
EcoT22I ATGCAT 2 cut(s) 1969, 2889
EcoT38I GRGCYC 2 cut(s) 246, 1022
ErhI CCWWGG 4 cut(s) 384, 1005, 1267, 3447
Esp3I CGTCTC 1 cut(s) 2721
FalI AAGNNNNNCTT 4 cut(s) 368, 400, 1147, 1179
FaqI GGGAC 3 cut(s) 3121, 3269, 3496
FauI CCCGC 3 cut(s) 1103, 1297, 3270
FauNDI CATATG 2 cut(s) 1547, 1963
FbaI TGATCA 2 cut(s) 301, 3307
FblI GTMKAC 2 cut(s) 2149, 3390
Fnu4HI GCNGC 6 cut(s) 33, 45, 477, 1115, 2865, 3733
FriOI GRGCYC 2 cut(s) 246, 1022
Fsp4HI GCNGC 6 cut(s) 33, 45, 477, 1115, 2865, 3733
FspBI CTAG 5 cut(s) 833, 1340, 1487, 1503, 1979
FspI TGCGCA 1 cut(s) 173
GlaI GCGC 4 cut(s) 173, 480, 1061, 3361
GluI GCNGC 6 cut(s) 33, 45, 477, 1115, 2865, 3733
GsuI CTGGAG 1 cut(s) 612
HaeII RGCGCY 2 cut(s) 482, 1063
HaeIII GGCC 6 cut(s) 1308, 1929, 2423, 2573, 2708, 3502
HapII CCGG 2 cut(s) 2574, 3007
HhaI GCGC 4 cut(s) 174, 481, 1062, 3362
Hin6I GCGC 4 cut(s) 172, 479, 1060, 3360
HinP1I GCGC 4 cut(s) 172, 479, 1060, 3360
HincII GTYRAC 2 cut(s) 728, 3384
HindII GTYRAC 2 cut(s) 728, 3384
HindIII AAGCTT 1 cut(s) 1932
HpaII CCGG 2 cut(s) 2574, 3007
HphI GGTGA 4 cut(s) 919, 1453, 2570, 2653
Hpy166II GTNNAC 8 cut(s) 728, 1575, 2150, 2497, 2736, 2773, 3384, 3391
Hpy8I GTNNAC 8 cut(s) 728, 1575, 2150, 2497, 2736, 2773, 3384, 3391
HpyF10VI GCNNNNNNNGC 5 cut(s) 41, 1483, 1657, 2308, 3359
HspAI GCGC 4 cut(s) 172, 479, 1060, 3360
Ksp22I TGATCA 2 cut(s) 301, 3307
LmnI GCTCC 5 cut(s) 41, 529, 1017, 1057, 1952
Lsp1109I GCAGC 6 cut(s) 19, 56, 488, 1126, 2851, 3719
LweI GCATC 6 cut(s) 511, 561, 964, 965, 1010, 3019
MaeI CTAG 5 cut(s) 833, 1340, 1487, 1503, 1979
MbiI CCGCTC 1 cut(s) 1846
MfeI CAATTG 2 cut(s) 1883, 1942
MflI RGATCY 2 cut(s) 323, 1036
MhlI GDGCHC 3 cut(s) 246, 1022, 3071
MlsI TGGCCA 2 cut(s) 1929, 2423
MluNI TGGCCA 2 cut(s) 1929, 2423
MlyI GAGTC 6 cut(s) 457, 976, 1868, 1900, 2608, 3077
MmeI TCCRAC 9 cut(s) 4, 63, 181, 297, 321, 493, 524, 1995, 3167
Mox20I TGGCCA 2 cut(s) 1929, 2423
Mph1103I ATGCAT 2 cut(s) 1969, 2889
MroXI GAANNNNTTC 4 cut(s) 792, 807, 861, 1038
MscI TGGCCA 2 cut(s) 1929, 2423
MslI CAYNNNNRTG 5 cut(s) 972, 1088, 2417, 2624, 3093
Msp20I TGGCCA 2 cut(s) 1929, 2423
MspA1I CMGCKG 1 cut(s) 151
MspCI CTTAAG 2 cut(s) 329, 731
MspI CCGG 2 cut(s) 2574, 3007
MspR9I CCNGG 2 cut(s) 591, 1388
MssI GTTTAAAC 1 cut(s) 103
MunI CAATTG 2 cut(s) 1883, 1942
Mva1269I GAATGC 1 cut(s) 47
MvaI CCWGG 2 cut(s) 591, 1388
MvnI CGCG 1 cut(s) 3362
MwoI GCNNNNNNNGC 5 cut(s) 41, 1483, 1657, 2308, 3359
NcoI CCATGG 1 cut(s) 3447
NdeI CATATG 2 cut(s) 1547, 1963
NheI GCTAGC 1 cut(s) 1486
NlaIV GGNNCC 5 cut(s) 325, 747, 1512, 2382, 2438
NmeAIII GCCGAG 1 cut(s) 2734
NmuCI GTSAC 7 cut(s) 907, 1101, 1459, 1759, 2467, 2617, 3080
NsbI TGCGCA 1 cut(s) 173
NsiI ATGCAT 2 cut(s) 1969, 2889
NspI RCATGY 3 cut(s) 1357, 1660, 3092
NspV TTCGAA 1 cut(s) 250
OliI CACNNNNGTG 1 cut(s) 2624
PasI CCCWGGG 1 cut(s) 1387
PceI AGGCCT 1 cut(s) 3502
PctI GAATGC 1 cut(s) 47
PdmI GAANNNNTTC 4 cut(s) 792, 807, 861, 1038
PfeI GAWTC 8 cut(s) 137, 209, 857, 1175, 1293, 1833, 2510, 3242
PflMI CCANNNNNTGG 2 cut(s) 1393, 1572
PfoI TCCNGGA 1 cut(s) 589
PkrI GCNGC 6 cut(s) 34, 46, 478, 1116, 2866, 3734
PleI GAGTC 6 cut(s) 456, 976, 1868, 1899, 2607, 3077
PmeI GTTTAAAC 1 cut(s) 103
PpsI GAGTC 6 cut(s) 456, 976, 1868, 1899, 2607, 3077
PshAI GACNNNNGTC 1 cut(s) 1244
PshBI ATTAAT 1 cut(s) 1869
Psp124BI GAGCTC 2 cut(s) 246, 1022
Psp6I CCWGG 2 cut(s) 589, 1386
PspGI CCWGG 2 cut(s) 589, 1386
PspN4I GGNNCC 5 cut(s) 325, 747, 1512, 2382, 2438
PspPI GGNCC 3 cut(s) 587, 829, 1306
PsrI GAACNNNNNNTAC 4 cut(s) 1982, 2014, 2373, 2405
PstI CTGCAG 2 cut(s) 775, 1899
PstNI CAGNNNCTG 2 cut(s) 482, 1393
PsuI RGATCY 2 cut(s) 323, 1036
RsaI GTAC 7 cut(s) 444, 579, 1881, 3317, 3540, 3614, 3645
RsaNI GTAC 7 cut(s) 443, 578, 1880, 3316, 3539, 3613, 3644
RseI CAYNNNNRTG 5 cut(s) 972, 1088, 2417, 2624, 3093
SacI GAGCTC 2 cut(s) 246, 1022
SatI GCNGC 6 cut(s) 33, 45, 477, 1115, 2865, 3733
Sau96I GGNCC 3 cut(s) 587, 829, 1306
ScaI AGTACT 1 cut(s) 3614
SchI GAGTC 6 cut(s) 457, 976, 1868, 1900, 2608, 3077
ScrFI CCNGG 2 cut(s) 591, 1388
SduI GDGCHC 3 cut(s) 246, 1022, 3071
SfaNI GCATC 6 cut(s) 511, 561, 964, 965, 1010, 3019
SfcI CTRYAG 5 cut(s) 280, 771, 1895, 2123, 2221
SfuI TTCGAA 1 cut(s) 250
SinI GGWCC 2 cut(s) 587, 829
SmiMI CAYNNNNRTG 5 cut(s) 972, 1088, 2417, 2624, 3093
SmlI CTYRAG 8 cut(s) 113, 329, 554, 731, 2131, 2330, 3414, 3616
SmoI CTYRAG 8 cut(s) 113, 329, 554, 731, 2131, 2330, 3414, 3616
SpeI ACTAGT 1 cut(s) 1502
SseBI AGGCCT 1 cut(s) 3502
SspMI CTAG 5 cut(s) 833, 1340, 1487, 1503, 1979
SstI GAGCTC 2 cut(s) 246, 1022
StuI AGGCCT 1 cut(s) 3502
StyD4I CCNGG 2 cut(s) 589, 1386
StyI CCWWGG 4 cut(s) 384, 1005, 1267, 3447
TaqI TCGA 5 cut(s) 250, 569, 715, 2174, 3289
TatI WGTACW 4 cut(s) 577, 1879, 3612, 3643
TfiI GAWTC 8 cut(s) 137, 209, 857, 1175, 1293, 1833, 2510, 3242
TscAI CASTG 5 cut(s) 304, 966, 2294, 3158, 3580
TseFI GTSAC 7 cut(s) 907, 1101, 1459, 1759, 2467, 2617, 3080
TseI GCWGC 6 cut(s) 32, 44, 476, 1114, 2864, 3732
Tsp45I GTSAC 7 cut(s) 907, 1101, 1459, 1759, 2467, 2617, 3080
TspGWI ACGGA 4 cut(s) 2774, 2870, 3516, 3639
TspRI CASTG 5 cut(s) 304, 966, 2294, 3158, 3580
Van91I CCANNNNNTGG 2 cut(s) 1393, 1572
Vha464I CTTAAG 2 cut(s) 329, 731
VpaK11BI GGWCC 2 cut(s) 587, 829
VspI ATTAAT 1 cut(s) 1869
XapI RAATTY 4 cut(s) 547, 1721, 2170, 3208
XceI RCATGY 3 cut(s) 1357, 1660, 3092
XcmI CCANNNNNNNNNTGG 1 cut(s) 2322
XmiI GTMKAC 2 cut(s) 2149, 3390
XmnI GAANNNNTTC 4 cut(s) 792, 807, 861, 1038
XspI CTAG 5 cut(s) 833, 1340, 1487, 1503, 1979
ZrmI AGTACT 1 cut(s) 3614
Zsp2I ATGCAT 2 cut(s) 1969, 2889
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.