FvH4_6g42370

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000039
Physical Location & Seq
Forward (+)
33052649 .. 33058190
5542 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g42370.t1

Sequence Viewer

Length: 1884 bp
ATGTTTAGAAGTCTCTTCAAAGTCATCAGATCCAGGCTAGACACTCTAAAGCCACTTTTCATCGACATGGCACTGTCTAACGAGCAACTGTCTTACACAAACAACGATGAGCTTAGAGGTTTAGAAAAAGTACTCAAGGTTGGCGAAAGGCTTGTTCTGAAGTGCTCAAATGTTCGTCTGTGGGATCTCTACAGAAAGTACAAATATGCCACAAAACTTCTTGCCTGGGAAGAAGAACTTGAAAGACAACTGCAACTGGGGAAGAAAACCGCGGTGGTAGTAGAGAACATAGAGGTTTCTGTAGGGAGTGTGAAGGAGGGGATCAACCAAATGTCTAGTACTCTGGGGGAGGTGAACCAACTCATGAAAGTGAATTTTGTGGTACAAGACCAGCCTGCTGAGAGTAGAGCTTGGTGCGCAGTACCTGAACTTCCAGAGGTTATAGTTGGACTGGAAGCTCCTTTGAAGGATTTGAAGACAAAGCTTCTTGGGAATGATGGATTCTCAATGCTTGTGCTCACTGCTCCCGGAGGATGTGGGAAAACTACCTTGGCAACCAACTTTTGCAAAGATCAGGAAGTCAGAGATAAATTAGGCAAAAATATCTTCTTTGTCACTGTTTCGAAGAAGTACAGTTTTAGCTTTATTGTAGAACAACTATATCGACACAACGGTTCACAAGTTCCTACTTTCCAAAACGAAGTGATTGCAGTTCAGTGGCTTCAAGCATTTTTAAAGGAAGTAGGAAAGAAACCTTTACTGTTTGTCCTGCATGGTGTTTGGTCCGGATCAGAATCTCTTTTTGATAAATTTGAATGCAGAATGGCAAATATTAGGATTCTTGTGACATCAAGATCTGAATTTCGAGCATTCAGTCCGCCATATCATTTAGAAATGCTGGGTTATGATGATGCAATGAAACTTTTTCATCATTCAGCGTCCTTGGGAGATAAGAGCTCTCATATTCCACCACATCTTTCAAGAAAGATAGTAAATCATTGTAAGGGATTTCCACTAGCCATTAAAGTGGTAGGGAGATCGCTTTGTGCACAACCTATAGAGATGTGGCAAAACAGACTAAATCAATGGTCTAAAGGTTCTTCTATTTTGGATTTTGAGACTGATTTACTTCTTTCCCTCCAAAGCAGCTTGGATGCTTTGGATAAGGAAAAGGCTATCATCAAAGAGTGTTTCTTGGACCTTGGTTCATTTCCTGAAGACAAAAATGTCCCGGTTAATGCCCTCATTGATATATGGTCCGAGTTATATGATATAGATGAAGACACTTCCTGCATTTCAAGCCTCTATGAACTCAGCAATCGGAGTCTGGCTAATCTTATTGACAAGAGGCGAACGAAGTTGGATGGAGAAGGATATTATGGTGAACACTATGTCACCCAACATGATTTGCTTAGGGAGCTAGCTATTTATCACTCAAAACTAGATCCGGTAGAGCACAGAGAAAGATTGATCATAGACATATGTGGAAGCAATCTTCCCAAGTGGTGGAGGGAACAAAAGTACCAAGTTATCAAAGCTCGCCTGGTATCTATATCAACTGATGCAGAGTCTTCAACTTTGAAATTGCCCAACATGCATCTGCCAGAAGCAGAGGTACTAGTCTTGAATCTGCGGGAGACACAGAAATATGCTATACCTGAATTTGTGGAGGAAATGGTGAAGTTGAAGCCCAGCAATAGGGCTCCACTCTTGCGTCAGCCAAGTGAAAAAACAACTGACGCGGGTTTTACATGCGTGTGTTTCATGGAGCACTACTATCCTTTCCTATTCACGTGTGTTGCACTTGCTGTTGCAACATACAAGACAAACCCCAACTCTCCAAAGAAATGTGTCAAAAGGCTGTTGGTTGGTTTTAATATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

628

Amino Acids

71.55

Weight (kDa)

8.06

Isoelectric Point (pI)

40.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 1 - 86 3.2e-11 Arabidopsis broad-spectrum mildew resistance protein RPW8
NB-ARC PF00931 152 - 312 1.9e-12 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000039)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66890 AT5G66900 AT5G66900 AT5G66900 AT5G66910
fragaria_vesca FvH4_1g15090 FvH4_1g15120 FvH4_1g15130 FvH4_1g15140 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15170 FvH4_1g15171 FvH4_1g15171 FvH4_1g15200 FvH4_1g15200 FvH4_1g15200 FvH4_1g15210 FvH4_1g15210 FvH4_1g15210 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15230 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15500 FvH4_1g15500 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_2g19760 FvH4_3g12820 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_6g18970 FvH4_6g42370 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g50100 FvH4_6g50100 FvH4_6g50110 FvH4_6g50130 FvH4_6g50140 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50330
malus_domestica MD00G1053700.v1.1 MD02G1164300.v1.1 MD02G1164400.v1.1 MD02G1164700.v1.1 MD02G1164800.v1.1 MD02G1165300.v1.1 MD02G1165400.v1.1 MD02G1165500.v1.1 MD02G1165600.v1.1 MD02G1165900.v1.1 MD02G1166000.v1.1 MD02G1166100.v1.1 MD02G1166300.v1.1 MD08G1084900.v1.1 MD15G1263100.v1.1 MD15G1263300.v1.1 MD15G1276900.v1.1 MD15G1277200.v1.1 MD15G1277400.v1.1 MD15G1277700.v1.1 MD15G1277800.v1.1 MD15G1278000.v1.1 MD15G1278100.v1.1 MD15G1278400.v1.1 MD15G1278600.v1.1
prunus_persica Prupe.7G138500_v2.0.a1 Prupe.7G138500_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138700_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138900_v2.0.a1 Prupe.7G139100_v2.0.a1 Prupe.7G139200_v2.0.a1 Prupe.7G139500_v2.0.a1 Prupe.7G139600_v2.0.a1 Prupe.7G139700_v2.0.a1 Prupe.7G139800_v2.0.a1
pyrus_communis pycom02g13150 pycom02g13160 pycom02g13190 pycom02g13200 pycom02g13220 pycom02g13230 pycom02g13250 pycom02g13260 pycom02g13270 pycom02g13300 pycom02g13310 pycom02g13330 pycom15g23980 pycom15g23990 pycom15g24030 pycom15g24040 pycom15g24050 pycom15g24070 pycom15g24090 pycom15g24160
rosa_chinensis RchiOBHm_Chr1g0326431 RchiOBHm_Chr1g0326451 RchiOBHm_Chr2g0104121 RchiOBHm_Chr2g0104151 RchiOBHm_Chr2g0104171 RchiOBHm_Chr2g0104181 RchiOBHm_Chr2g0104191 RchiOBHm_Chr2g0104201 RchiOBHm_Chr2g0104211 RchiOBHm_Chr2g0104241 RchiOBHm_Chr2g0104261 RchiOBHm_Chr2g0104281 RchiOBHm_Chr2g0104311 RchiOBHm_Chr2g0104331 RchiOBHm_Chr2g0104361 RchiOBHm_Chr2g0104381 RchiOBHm_Chr2g0104391 RchiOBHm_Chr2g0104431 RchiOBHm_Chr2g0104441 RchiOBHm_Chr2g0104541 RchiOBHm_Chr2g0104611 RchiOBHm_Chr2g0104741 RchiOBHm_Chr2g0104761 RchiOBHm_Chr2g0158291 RchiOBHm_Chr2g0170371 RchiOBHm_Chr2g0170631 RchiOBHm_Chr3g0473971 RchiOBHm_Chr3g0480491 RchiOBHm_Chr3g0480541 RchiOBHm_Chr3g0480561 RchiOBHm_Chr3g0485281 RchiOBHm_Chr3g0485291 RchiOBHm_Chr4g0411541 RchiOBHm_Chr6g0285471
rosa_laevigata RLG00000008347 RLG00000012637 RLG00000016961 RLG00000017293 RLG00000017305 RLG00000017308 RLG00000017312 RLG00000017314 RLG00000017317 RLG00000017318 RLG00000017319 RLG00000017321 RLG00000017322 RLG00000017323 RLG00000017324 RLG00000017325 RLG00000017327 RLG00000017328 RLG00000017329 RLG00000017330 RLG00000017334 RLG00000020833 RLG00000021073 RLG00000021950 RLG00000021971 RLG00000023154 RLG00000023157 RLG00000023467 RLG00000023468 RLG00000023525 RLG00000023963 RLG00000033265 RLG00000033267 RLG00000033268
rosa_multiflora Rmu_co8053082.1_g000001 Rmu_co8210358.1_g000001 Rmu_co8331661.1_g000001 Rmu_co8477427.1_g000002 Rmu_co8483217.1_g000001 Rmu_sc0000284.1_g000004 Rmu_sc0000676.1_g000017 Rmu_sc0000676.1_g000019 Rmu_sc0000676.1_g000021 Rmu_sc0002205.1_g000038 Rmu_sc0002205.1_g000041 Rmu_sc0002316.1_g000010 Rmu_sc0002316.1_g000012 Rmu_sc0002316.1_g000040 Rmu_sc0002316.1_g000071 Rmu_sc0002316.1_g000072 Rmu_sc0002316.1_g000095 Rmu_sc0002316.1_g000102 Rmu_sc0002414.1_g000018 Rmu_sc0002576.1_g000002 Rmu_sc0002576.1_g000003 Rmu_sc0002687.1_g000010 Rmu_sc0002687.1_g000017 Rmu_sc0003660.1_g000001 Rmu_sc0003787.1_g000014 Rmu_sc0003787.1_g000020 Rmu_sc0003787.1_g000024 Rmu_sc0003787.1_g000029 Rmu_sc0004059.1_g000026 Rmu_sc0005137.1_g000021 Rmu_sc0006550.1_g000001 Rmu_sc0006561.1_g000025 Rmu_sc0006561.1_g000033 Rmu_sc0007704.1_g000005 Rmu_sc0008904.1_g000005 Rmu_sc0010270.1_g000002 Rmu_sc0010307.1_g000001 Rmu_sc0011601.1_g000013 Rmu_sc0012694.1_g000005 Rmu_sc0013101.1_g000008 Rmu_sc0019029.1_g000001 Rmu_sc0019728.1_g000001 Rmu_sc0025829.1_g000001 Rmu_sc0025829.1_g000002 Rmu_ssc0000257.1_g000007 Rmu_ssc0000257.1_g000008
rosa_roxburghii Rroxscaffold_1G00021710 Rroxscaffold_1G00049350 Rroxscaffold_1G00049360 Rroxscaffold_2G00081160 Rroxscaffold_2G00081360 Rroxscaffold_2G00081390 Rroxscaffold_2G00091540 Rroxscaffold_2G00138790 Rroxscaffold_2G00138800 Rroxscaffold_2G00138920 Rroxscaffold_2G00139000 Rroxscaffold_2G00139010 Rroxscaffold_2G00139020 Rroxscaffold_2G00139030 Rroxscaffold_2G00139040 Rroxscaffold_2G00139050 Rroxscaffold_2G00139060 Rroxscaffold_2G00139070 Rroxscaffold_2G00139090 Rroxscaffold_2G00139100 Rroxscaffold_3G00257470 Rroxscaffold_4G00318040 Rroxscaffold_4G00323740 Rroxscaffold_4G00323750 Rroxscaffold_5G00356140 Rroxscaffold_6G00401280 Rroxscaffold_6G00401290 Rroxscaffold_7G00183120
rosa_rugosa Rorug01G0060500 Rorug01G0060900 Rorug02G0119500 Rorug02G0119600 Rorug02G0119700 Rorug02G0119700 Rorug02G0119700 Rorug02G0119800.1 Rorug02G0119900.1 Rorug02G0120000 Rorug02G0120100 Rorug02G0120200 Rorug02G0120300 Rorug02G0120400 Rorug02G0120500 Rorug02G0120700 Rorug02G0120700 Rorug02G0122300 Rorug02G0122300 Rorug02G0122400.1 Rorug02G0466700 Rorug02G0466700 Rorug02G0466800 Rorug02G0548600 Rorug02G0549900 Rorug02G0550000 Rorug03G0184200 Rorug04G0107900 Rorug05G0121500 Rorug06G0174800
rosa_samantha Rh1AG198400 Rh1CG074700 Rh1CG075000 Rh1CG183700 Rh2AG169700 Rh2AG169800 Rh2AG169900 Rh2AG170000 Rh2AG170300 Rh2AG170400 Rh2AG171000 Rh2AG171100 Rh2AG171600 Rh2AG172400 Rh2AG172500 Rh2AG532900 Rh2AG620000 Rh2AG622500 Rh2AG622800 Rh2AG623000 Rh2BG176200 Rh2BG176300 Rh2BG176400 Rh2BG176500 Rh2BG176600 Rh2BG176900 Rh2BG177000 Rh2BG177100 Rh2BG177200 Rh2BG177300 Rh2BG177600 Rh2BG177700 Rh2BG177900 Rh2BG178000 Rh2BG178200 Rh2BG178300 Rh2BG178500 Rh2BG179000 Rh2BG180000 Rh2BG180100 Rh2BG259200 Rh2BG546700 Rh2BG630800 Rh2BG633500 Rh2CG176500 Rh2CG177500 Rh2CG177700 Rh2DG174400 Rh2DG174600 Rh2DG174900 Rh2DG175100 Rh2DG175200 Rh2DG175300 Rh2DG175600 Rh2DG175700 Rh2DG175800 Rh2DG175900 Rh2DG176000 Rh2DG176300 Rh2DG176400 Rh2DG176500 Rh2DG176600 Rh2DG176900 Rh2DG177100 Rh2DG177200 Rh2DG177800 Rh2DG177900 Rh2DG178700 Rh2DG178800 Rh2DG555700 Rh2DG643700 Rh2DG646300 Rh3AG235600 Rh3AG235900 Rh3AG236200 Rh3AG266700 Rh3AG266900 Rh3BG216200 Rh3BG269100 Rh3CG212700 Rh3CG265800 Rh4AG168900 Rh4BG165500 Rh4CG180300 Rh5BG261500 Rh6AG286300 Rh6BG287500 Rh6CG288600 Rh7AG022600
rosa_wichuraiana Rw0G008240 Rw0G008250 Rw0G013190 Rw0G013200 Rw2G013230 Rw2G013240 Rw2G013270 Rw2G013280 Rw2G013290 Rw2G013300 Rw2G013330 Rw2G013340 Rw2G013350 Rw2G013360 Rw2G013370 Rw2G013380 Rw2G013410 Rw2G013570 Rw2G044120 Rw2G051370 Rw2G051590 Rw3G017160 Rw3G021150 Rw3G021160 Rw3G023730 Rw3G023740 Rw4G014030 Rw6G024590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1226
Acc16I TGCGCA 1 cut(s) 418
AccB7I CCANNNNNTGG 1 cut(s) 1506
AccII CGCG 2 cut(s) 272, 1742
AccIII TCCGGA 1 cut(s) 785
AciI CCGC 5 cut(s) 270, 272, 878, 1633, 1742
AclWI GGATC 5 cut(s) 24, 192, 329, 796, 1439
AcsI RAATTY 4 cut(s) 373, 809, 860, 1661
AcuI CTGAAG 2 cut(s) 179, 1236
AcvI CACGTG 1 cut(s) 1794
AfaI GTAC 8 cut(s) 132, 200, 340, 384, 423, 632, 1523, 1617
AfiI CCNNNNNNNGG 2 cut(s) 1506, 1698
AflIII ACRYGT 1 cut(s) 1793
AhlI ACTAGT 1 cut(s) 1618
AjnI CCWGG 3 cut(s) 32, 224, 1542
AjuI GAANNNNNNNTTGG 2 cut(s) 533, 565
Alw21I GWGCWC 6 cut(s) 167, 519, 959, 1051, 1458, 1773
Alw26I GTCTC 3 cut(s) 17, 1112, 1631
Alw44I GTGCAC 1 cut(s) 1047
AlwI GGATC 5 cut(s) 24, 192, 329, 796, 1439
AlwNI CAGNNNCTG 1 cut(s) 425
Aor13HI TCCGGA 1 cut(s) 785
ApaLI GTGCAC 1 cut(s) 1047
ApeKI GCWGC 1 cut(s) 1146
ApoI RAATTY 4 cut(s) 373, 809, 860, 1661
AspLEI GCGC 1 cut(s) 419
AspS9I GGNCC 3 cut(s) 783, 1198, 1257
AsuC2I CCSGG 2 cut(s) 528, 1232
AsuHPI GGTGA 4 cut(s) 364, 1387, 1394, 1690
AsuII TTCGAA 1 cut(s) 623
AsuNHI GCTAGC 1 cut(s) 1420
AvaII GGWCC 3 cut(s) 783, 1198, 1257
BaeGI GKGCMC 1 cut(s) 1051
BaeI ACNNNNGTAYC 2 cut(s) 1505, 1538
BanII GRGCYC 2 cut(s) 959, 1705
BarI GAAGNNNNNNTAC 4 cut(s) 414, 446, 1599, 1631
BbrPI CACGTG 1 cut(s) 1794
BbsI GAAGAC 4 cut(s) 482, 1224, 1287, 1563
Bbv12I GWGCWC 6 cut(s) 167, 519, 959, 1051, 1458, 1773
BbvI GCAGC 1 cut(s) 1158
BccI CCATC 2 cut(s) 491, 1358
BcgI CGANNNNNNTGC 2 cut(s) 689, 723
BciT130I CCWGG 3 cut(s) 34, 226, 1544
BclI TGATCA 1 cut(s) 1470
BcnI CCSGG 2 cut(s) 528, 1232
BcoDI GTCTC 3 cut(s) 17, 1112, 1631
BcuI ACTAGT 1 cut(s) 1618
BfaI CTAG 6 cut(s) 38, 336, 1016, 1421, 1442, 1619
BfmI CTRYAG 3 cut(s) 190, 300, 1056
BglII AGATCT 1 cut(s) 854
BisI GCNGC 1 cut(s) 1147
BlsI GCNGC 1 cut(s) 1148
BmcAI AGTACT 2 cut(s) 132, 340
Bme1390I CCNGG 5 cut(s) 34, 226, 528, 1232, 1544
Bme18I GGWCC 3 cut(s) 783, 1198, 1257
BmgT120I GGNCC 3 cut(s) 783, 1198, 1257
BmiI GGNNCC 1 cut(s) 1704
BmrFI CCNGG 5 cut(s) 34, 226, 528, 1232, 1544
BmrI ACTGGG 1 cut(s) 266
BmsI GCATC 4 cut(s) 901, 1144, 1552, 1606
BmtI GCTAGC 1 cut(s) 1424
BmuI ACTGGG 1 cut(s) 266
BpiI GAAGAC 4 cut(s) 482, 1224, 1287, 1563
Bpu10I CCTNAGC 1 cut(s) 1412
Bpu14I TTCGAA 1 cut(s) 623
BpuEI CTTGAG 1 cut(s) 119
BpuMI CCSGG 2 cut(s) 528, 1232
BsaAI YACGTR 1 cut(s) 1794
BsaBI GATNNNNATC 1 cut(s) 793
BsaJI CCNNGG 5 cut(s) 225, 270, 549, 942, 1201
BsaWI WCCGGW 2 cut(s) 785, 1447
Bsc4I CCNNNNNNNGG 2 cut(s) 1506, 1698
Bse1I ACTGG 2 cut(s) 261, 456
Bse3DI GCAATG 1 cut(s) 921
Bse8I GATNNNNATC 1 cut(s) 793
BseAI TCCGGA 1 cut(s) 785
BseBI CCWGG 3 cut(s) 34, 226, 1544
BseDI CCNNGG 5 cut(s) 225, 270, 549, 942, 1201
BseGI GGATG 3 cut(s) 539, 1159, 1369
BseJI GATNNNNATC 1 cut(s) 793
BseLI CCNNNNNNNGG 2 cut(s) 1506, 1698
BseMI GCAATG 1 cut(s) 921
BseMII CTCAG 2 cut(s) 390, 1327
BseNI ACTGG 2 cut(s) 261, 456
BseSI GKGCMC 1 cut(s) 1051
BseXI GCAGC 1 cut(s) 1158
BseYI CCCAGC 2 cut(s) 898, 1691
Bsh1236I CGCG 2 cut(s) 272, 1742
BsiHKAI GWGCWC 6 cut(s) 167, 519, 959, 1051, 1458, 1773
BsiSI CCGG 4 cut(s) 528, 786, 1232, 1448
BslFI GGGAC 1 cut(s) 1214
BslI CCNNNNNNNGG 2 cut(s) 1506, 1698
BsmAI GTCTC 3 cut(s) 17, 1112, 1631
BsmFI GGGAC 1 cut(s) 1214
BsmI GAATGC 2 cut(s) 821, 869
Bsp119I TTCGAA 1 cut(s) 623
Bsp1286I GDGCHC 7 cut(s) 167, 519, 959, 1051, 1458, 1705, 1773
Bsp13I TCCGGA 1 cut(s) 785
Bsp143I GATC 9 cut(s) 29, 184, 321, 571, 788, 854, 1037, 1444, 1470
BspACI CCGC 5 cut(s) 270, 272, 878, 1633, 1742
BspCNI CTCAG 2 cut(s) 391, 1326
BspEI TCCGGA 1 cut(s) 785
BspFNI CGCG 2 cut(s) 272, 1742
BspHI TCATGA 1 cut(s) 363
BspLI GGNNCC 1 cut(s) 1704
BspOI GCTAGC 1 cut(s) 1424
BspPI GGATC 5 cut(s) 24, 192, 329, 796, 1439
BspT104I TTCGAA 1 cut(s) 623
BsrDI GCAATG 1 cut(s) 921
BsrI ACTGG 2 cut(s) 261, 456
BssECI CCNNGG 5 cut(s) 225, 270, 549, 942, 1201
BssMI GATC 9 cut(s) 29, 184, 321, 571, 788, 854, 1037, 1444, 1470
BssT1I CCWWGG 3 cut(s) 549, 942, 1201
Bst2UI CCWGG 3 cut(s) 34, 226, 1544
Bst4CI ACNGT 6 cut(s) 75, 90, 619, 635, 674, 762
Bst6I CTCTTC 1 cut(s) 20
BstBAI YACGTR 1 cut(s) 1794
BstBI TTCGAA 1 cut(s) 623
BstC8I GCNNGC 3 cut(s) 396, 1422, 1540
BstDEI CTNAG 4 cut(s) 113, 399, 1313, 1412
BstDSI CCRYGG 1 cut(s) 270
BstF5I GGATG 3 cut(s) 539, 1159, 1369
BstFNI CGCG 2 cut(s) 272, 1742
BstHHI GCGC 1 cut(s) 419
BstKTI GATC 9 cut(s) 32, 187, 324, 574, 791, 857, 1040, 1447, 1473
BstMAI GTCTC 3 cut(s) 17, 1112, 1631
BstMBI GATC 9 cut(s) 29, 184, 321, 571, 788, 854, 1037, 1444, 1470
BstMWI GCNNNNNNNGC 4 cut(s) 416, 1299, 1417, 1594
BstNI CCWGG 3 cut(s) 34, 226, 1544
BstNSI RCATGY 2 cut(s) 1597, 1755
BstSCI CCNGG 5 cut(s) 32, 224, 526, 1230, 1542
BstSFI CTRYAG 3 cut(s) 190, 300, 1056
BstSLI GKGCMC 1 cut(s) 1051
BstUI CGCG 2 cut(s) 272, 1742
BstV1I GCAGC 1 cut(s) 1158
BstV2I GAAGAC 4 cut(s) 482, 1224, 1287, 1563
BstX2I RGATCY 4 cut(s) 29, 184, 854, 1444
BstXI CCANNNNNNTGG 1 cut(s) 1027
BstYI RGATCY 4 cut(s) 29, 184, 854, 1444
BtgI CCRYGG 1 cut(s) 270
BtsCI GGATG 3 cut(s) 539, 1159, 1369
BtsI GCAGTG 1 cut(s) 519
BtsIMutI CAGTG 4 cut(s) 71, 519, 615, 722
Cac8I GCNNGC 3 cut(s) 396, 1422, 1540
CaiI CAGNNNCTG 1 cut(s) 425
CciI TCATGA 1 cut(s) 363
CfoI GCGC 1 cut(s) 419
Cfr13I GGNCC 3 cut(s) 783, 1198, 1257
Cfr42I CCGCGG 1 cut(s) 273
CseI GACGC 3 cut(s) 927, 1703, 1748
Csp6I GTAC 8 cut(s) 131, 199, 339, 383, 422, 631, 1522, 1616
CviAII CATG 7 cut(s) 67, 364, 773, 1403, 1594, 1752, 1765
CviQI GTAC 8 cut(s) 131, 199, 339, 383, 422, 631, 1522, 1616
DdeI CTNAG 4 cut(s) 113, 399, 1313, 1412
DpnI GATC 9 cut(s) 31, 186, 323, 573, 790, 856, 1039, 1446, 1472
DpnII GATC 9 cut(s) 29, 184, 321, 571, 788, 854, 1037, 1444, 1470
DraI TTTAAA 1 cut(s) 735
DrdI GACNNNNNNGTC 1 cut(s) 1226
DseDI GACNNNNNNGTC 1 cut(s) 1226
Eam1104I CTCTTC 1 cut(s) 20
EarI CTCTTC 1 cut(s) 20
EciI GGCGGA 1 cut(s) 867
Ecl136II GAGCTC 1 cut(s) 957
Eco130I CCWWGG 3 cut(s) 549, 942, 1201
Eco24I GRGCYC 2 cut(s) 959, 1705
Eco47I GGWCC 3 cut(s) 783, 1198, 1257
Eco53kI GAGCTC 1 cut(s) 957
Eco57I CTGAAG 2 cut(s) 179, 1236
Eco72I CACGTG 1 cut(s) 1794
EcoICRI GAGCTC 1 cut(s) 957
EcoRII CCWGG 3 cut(s) 32, 224, 1542
EcoT14I CCWWGG 3 cut(s) 549, 942, 1201
EcoT22I ATGCAT 1 cut(s) 1599
EcoT38I GRGCYC 2 cut(s) 959, 1705
ErhI CCWWGG 3 cut(s) 549, 942, 1201
FaeI CATG 7 cut(s) 70, 367, 776, 1406, 1597, 1755, 1768
FalI AAGNNNNNCTT 2 cut(s) 222, 254
FaqI GGGAC 1 cut(s) 1214
FatI CATG 7 cut(s) 66, 363, 772, 1402, 1593, 1751, 1764
FauI CCCGC 2 cut(s) 1626, 1735
FauNDI CATATG 1 cut(s) 1481
FbaI TGATCA 1 cut(s) 1470
Fnu4HI GCNGC 1 cut(s) 1147
FokI GGATG 3 cut(s) 546, 1166, 1376
FriOI GRGCYC 2 cut(s) 959, 1705
Fsp4HI GCNGC 1 cut(s) 1147
FspBI CTAG 6 cut(s) 38, 336, 1016, 1421, 1442, 1619
FspI TGCGCA 1 cut(s) 418
GlaI GCGC 1 cut(s) 418
GluI GCNGC 1 cut(s) 1147
GsaI CCCAGC 2 cut(s) 902, 1695
HapII CCGG 4 cut(s) 528, 786, 1232, 1448
HgaI GACGC 3 cut(s) 927, 1703, 1748
HhaI GCGC 1 cut(s) 419
Hin1II CATG 7 cut(s) 70, 367, 776, 1406, 1597, 1755, 1768
Hin6I GCGC 1 cut(s) 417
HinP1I GCGC 1 cut(s) 417
HindIII AAGCTT 1 cut(s) 482
HinfI GANTC 6 cut(s) 501, 794, 838, 1324, 1568, 1627
HpaII CCGG 4 cut(s) 528, 786, 1232, 1448
HphI GGTGA 4 cut(s) 364, 1387, 1394, 1690
Hpy166II GTNNAC 4 cut(s) 355, 677, 1049, 1385
Hpy188I TCNGA 7 cut(s) 29, 159, 584, 793, 859, 1261, 1323
Hpy188III TCNNGA 8 cut(s) 364, 434, 575, 786, 852, 981, 1214, 1624
Hpy8I GTNNAC 4 cut(s) 355, 677, 1049, 1385
HpyAV CCTTC 3 cut(s) 307, 460, 1364
HpyCH4III ACNGT 6 cut(s) 75, 90, 619, 635, 674, 762
HpyCH4IV ACGT 1 cut(s) 1793
HpyF10VI GCNNNNNNNGC 4 cut(s) 416, 1299, 1417, 1594
HpyF3I CTNAG 4 cut(s) 113, 399, 1313, 1412
HpySE526I ACGT 1 cut(s) 1793
Hsp92II CATG 7 cut(s) 70, 367, 776, 1406, 1597, 1755, 1768
HspAI GCGC 1 cut(s) 417
Kpn2I TCCGGA 1 cut(s) 785
Ksp22I TGATCA 1 cut(s) 1470
KspI CCGCGG 1 cut(s) 273
Kzo9I GATC 9 cut(s) 29, 184, 321, 571, 788, 854, 1037, 1444, 1470
LmnI GCTCC 5 cut(s) 463, 529, 1417, 1708, 1768
Lsp1109I GCAGC 1 cut(s) 1158
LweI GCATC 4 cut(s) 901, 1144, 1552, 1606
MaeI CTAG 6 cut(s) 38, 336, 1016, 1421, 1442, 1619
MaeII ACGT 1 cut(s) 1793
MaeIII GTNAC 3 cut(s) 613, 844, 1393
MalI GATC 9 cut(s) 31, 186, 323, 573, 790, 856, 1039, 1446, 1472
MboI GATC 9 cut(s) 29, 184, 321, 571, 788, 854, 1037, 1444, 1470
MflI RGATCY 4 cut(s) 29, 184, 854, 1444
MhlI GDGCHC 7 cut(s) 167, 519, 959, 1051, 1458, 1705, 1773
MluCI AATT 6 cut(s) 373, 590, 809, 860, 1583, 1661
MlyI GAGTC 2 cut(s) 1333, 1577
MmeI TCCRAC 2 cut(s) 427, 1341
Mph1103I ATGCAT 1 cut(s) 1599
MroI TCCGGA 1 cut(s) 785
MseI TTAA 5 cut(s) 734, 1023, 1236, 1875, 1882
MslI CAYNNNNRTG 4 cut(s) 65, 368, 1025, 1756
MspA1I CMGCKG 1 cut(s) 272
MspI CCGG 4 cut(s) 528, 786, 1232, 1448
MspR9I CCNGG 5 cut(s) 34, 226, 528, 1232, 1544
Mva1269I GAATGC 2 cut(s) 821, 869
MvaI CCWGG 3 cut(s) 34, 226, 1544
MvnI CGCG 2 cut(s) 272, 1742
MwoI GCNNNNNNNGC 4 cut(s) 416, 1299, 1417, 1594
NciI CCSGG 2 cut(s) 528, 1232
NdeI CATATG 1 cut(s) 1481
NdeII GATC 9 cut(s) 29, 184, 321, 571, 788, 854, 1037, 1444, 1470
NheI GCTAGC 1 cut(s) 1420
NlaIII CATG 7 cut(s) 70, 367, 776, 1406, 1597, 1755, 1768
NlaIV GGNNCC 1 cut(s) 1704
NmuCI GTSAC 3 cut(s) 613, 844, 1393
NsbI TGCGCA 1 cut(s) 418
NsiI ATGCAT 1 cut(s) 1599
NspI RCATGY 2 cut(s) 1597, 1755
NspV TTCGAA 1 cut(s) 623
PagI TCATGA 1 cut(s) 363
PctI GAATGC 2 cut(s) 821, 869
PfeI GAWTC 4 cut(s) 501, 794, 838, 1627
PflMI CCANNNNNTGG 1 cut(s) 1506
PfoI TCCNGGA 1 cut(s) 526
PkrI GCNGC 1 cut(s) 1148
PleI GAGTC 2 cut(s) 1332, 1576
PmaCI CACGTG 1 cut(s) 1794
PmlI CACGTG 1 cut(s) 1794
PpsI GAGTC 2 cut(s) 1332, 1576
Ppu21I YACGTR 1 cut(s) 1794
Psp124BI GAGCTC 1 cut(s) 959
Psp6I CCWGG 3 cut(s) 32, 224, 1542
PspCI CACGTG 1 cut(s) 1794
PspFI CCCAGC 2 cut(s) 898, 1691
PspGI CCWGG 3 cut(s) 32, 224, 1542
PspN4I GGNNCC 1 cut(s) 1704
PspPI GGNCC 3 cut(s) 783, 1198, 1257
PstNI CAGNNNCTG 1 cut(s) 425
PsuI RGATCY 4 cut(s) 29, 184, 854, 1444
RsaI GTAC 8 cut(s) 132, 200, 340, 384, 423, 632, 1523, 1617
RsaNI GTAC 8 cut(s) 131, 199, 339, 383, 422, 631, 1522, 1616
RseI CAYNNNNRTG 4 cut(s) 65, 368, 1025, 1756
SacI GAGCTC 1 cut(s) 959
SacII CCGCGG 1 cut(s) 273
SaqAI TTAA 5 cut(s) 734, 1023, 1236, 1875, 1882
SatI GCNGC 1 cut(s) 1147
Sau3AI GATC 9 cut(s) 29, 184, 321, 571, 788, 854, 1037, 1444, 1470
Sau96I GGNCC 3 cut(s) 783, 1198, 1257
ScaI AGTACT 2 cut(s) 132, 340
SchI GAGTC 2 cut(s) 1333, 1577
ScrFI CCNGG 5 cut(s) 34, 226, 528, 1232, 1544
SduI GDGCHC 7 cut(s) 167, 519, 959, 1051, 1458, 1705, 1773
SfaNI GCATC 4 cut(s) 901, 1144, 1552, 1606
SfcI CTRYAG 3 cut(s) 190, 300, 1056
Sfr303I CCGCGG 1 cut(s) 273
SfuI TTCGAA 1 cut(s) 623
SgrBI CCGCGG 1 cut(s) 273
SinI GGWCC 3 cut(s) 783, 1198, 1257
SmiMI CAYNNNNRTG 4 cut(s) 65, 368, 1025, 1756
SmlI CTYRAG 1 cut(s) 134
SmoI CTYRAG 1 cut(s) 134
SpeI ACTAGT 1 cut(s) 1618
Sse9I AATT 6 cut(s) 373, 590, 809, 860, 1583, 1661
SsiI CCGC 5 cut(s) 270, 272, 878, 1633, 1742
SspI AATATT 2 cut(s) 832, 1879
SspMI CTAG 6 cut(s) 38, 336, 1016, 1421, 1442, 1619
SstI GAGCTC 1 cut(s) 959
StyD4I CCNGG 5 cut(s) 32, 224, 526, 1230, 1542
StyI CCWWGG 3 cut(s) 549, 942, 1201
TaaI ACNGT 6 cut(s) 75, 90, 619, 635, 674, 762
TaiI ACGT 1 cut(s) 1796
TaqI TCGA 4 cut(s) 63, 623, 664, 865
TasI AATT 6 cut(s) 373, 590, 809, 860, 1583, 1661
TatI WGTACW 4 cut(s) 130, 198, 338, 630
TfiI GAWTC 4 cut(s) 501, 794, 838, 1627
Tru1I TTAA 5 cut(s) 734, 1023, 1236, 1875, 1882
Tru9I TTAA 5 cut(s) 734, 1023, 1236, 1875, 1882
TscAI CASTG 4 cut(s) 78, 526, 622, 722
TseFI GTSAC 3 cut(s) 613, 844, 1393
TseI GCWGC 1 cut(s) 1146
Tsp45I GTSAC 3 cut(s) 613, 844, 1393
TspDTI ATGAA 8 cut(s) 49, 380, 917, 932, 1197, 1293, 1323, 1753
TspRI CASTG 4 cut(s) 78, 526, 622, 722
Van91I CCANNNNNTGG 1 cut(s) 1506
VneI GTGCAC 1 cut(s) 1047
VpaK11BI GGWCC 3 cut(s) 783, 1198, 1257
XapI RAATTY 4 cut(s) 373, 809, 860, 1661
XceI RCATGY 2 cut(s) 1597, 1755
XspI CTAG 6 cut(s) 38, 336, 1016, 1421, 1442, 1619
ZrmI AGTACT 2 cut(s) 132, 340
Zsp2I ATGCAT 1 cut(s) 1599
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.