Rw2G051590

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000039
Physical Location & Seq
Forward (+)
79282656 .. 79287147
4492 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G051590.1

Sequence Viewer

Length: 2457 bp
ATGGCCGTGGTGTCGGACTTTGTTCTAGAGAAAGCATTCGAAGCAGTGTATGTTGGCGTTGAAGACCTGATCGTCAAGAATTTCATTTTTAAGTCTCTCTTCAACGACGCCAAATCCAGGCTCGATGATTTGGCACCACTGATCAAGGAGATGGCGCAATACAACCAGAAAATGGGTCGCCCAGAGAAGGAACTGGAAGCTTTAAAGGGAGTCATGGAGAAAGGCCAAGAGCTCGTCAACAAGTGCTCAACGATTACGCGATGGAGCCCCTTCAAAAAGTACAAGTACGCCAGGAAACTTCTCCAATGGCAGGAATCTCTTAAAGCACCGTTGGAGAAACTCAAGGTGCAAGAATCAATGCAGGTAATGAGGATCGATGTCGCAGTTGATGATATTCATCGAATGATGCTGAAACAACAACCCGCAGAAAGTTCAAGTAGTACACGACTCGAACTTCCACCACTTCCACGGCTTATAGTTGGACTGGATGGTCCTTTGAATGATTTGAAGAAAAAGCTTCTTGCGGATGATCGGGTGTCGAGGCTTGTGCTCACTGCTCCTGGAGGCTGTGGGAAAACCACTCTCGCCACAATGTTTTGTCAAGATGAAGAGGTCAAAGGTAAATTCAAGGACAATATTTTCTTCATTACTGTTTCACAAAAAGGCAATTTGGAACTTGTTGTGAAACGACTTTGTGAATCCGTGAGTTCTCCGGTTCCTACTTTCCAAAACGAAGTACATATGCTTCAATGGATGCGAACATTTTTCAAGGGAAGACCAAATCCTTTGCTGTTGGTCCTCGATGATATTTGGTCTGGATCATCAGAATCCATTCTTCATAGCTTTCAATTTGAATTATCCAATTACAAGATTTTAGTAACCTCAAGATCTGAATTTCCAAATTTTGGTTCATCATATCATTTAAAATTATTGGATGATGACAAAGCTATGGAACTTTTTTATCACTCAACATCATTGGGGGATGAGCGCTCTCCTGAACGAGAAGAACTCTCAAGAAAGATAGTAAAGCGTTGCAACGGATTTCCACTTGCTATTACAGTGGTTGGAAGATCACTTTCTTGGCAACCTATAGAGGTCTGGAGAAAAAGAGTTTCTGAATGGTCTAAAGATTCTTCTATTCTAGATGTTGAGACGGAATTATTTCTTTGTCTCCAAAGCAGCTTAGATGCTTTGGATAAAAGACTTGCAATTGTCAAAGATTGTTACTTGGACCTGGGATCTTTTCCTGAAGACAAAGAAATCCGAGTTGATGACCTCATTGACATGTGGGCAGAGTTATATGATATAGATGATGACAGTGTGTGCTTTGCAAATGTCTATGAACTTGCCAATAGGAATCTGGCTAATCTGGTTGACATAAGGAACAGCCATATGAAGATGGAGGGGGACGATTACTACGGTGAACGGTTTGTCACCCAACATGATTTGCTTAGAGAACTAACTATTTACAATGCTAAGCTAGACCCGGTAGAGCAAAGACAAAGAATGAACATAGAAATAAGGGGAAACAAAATCCCCAAGTGGTGGAGAGAACAAAAATGTCACCCTATCAAGGCTCGCTTGTTATCTATTACTACAACTACTGACTTCGATATGGAATGGCCCAACTTGCAACTACCAGAAGCAGAAGTTTTTGTCTTGCATTTTCAGAGCCCAAGTAGTTACAGCTTGCCCGCGTTTGTACAGAAAATGGATAAATTGAAGGTACTGGTAATAGACGGCGATAGGCGATTAATAAATTTTGAACTACTCGGTTTGTTACCAAATCTAAAGAGAATCAGTTTCATTGGTCTCCGAATTGGTTTCTTGACCCACAAGCCCATACAACTGAAAAGTCTAAAGAAGCTATCTTTCGCGTCATGTGGCGCGGAGGGTATCGACGAAGGTTTTTTCAGAATGTCGAAAGCATTTCCGAATTTGGAAGAATTAAACATTCAGTTCCCCTCCCGTTCATTTGAAGAATTTCTTGTGGAGTGCTGCAATCTGATTCATTTAAAGAAGATTAGTATCTCCTCCTCCTCCTTTTCCGATCCGTTTGGGCCACGTTACTTGCCCGAAGAAATCGGAAATTTGGTCAACTTAGAAGTACTGAGGCTAAGGTCTTGTATTTATCTTTCAAAGCTTCCCAGTTCCATCAGCAAACTCAAAAAGTTAAACTTCCTTGACATATCTGGATGCCACCGCATTGAGTTGCCTAAAAACATTGGTGAATTGAGCAGTTTAAGAAAGTTGGACATGAGGAGTTGCAACAAAGTGAACGAGCTGCCTCCATCAGTTTCGGATCTTCATCAGTTAGAGGAAGTGATATGCAACCGTGGATACCAAGGCAGTTTATGGGAACCCTTTCGACCCACTCTCACAAACCTGCGCGTGGTGGTGATTCCTCGAACCCAACCAAATTGGTTGGAAACTTTCAGCGACGGTTGGAGCAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

818

Amino Acids

94.13

Weight (kDa)

6.71

Isoelectric Point (pI)

47.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 8 - 109 4.4e-17 Arabidopsis broad-spectrum mildew resistance protein RPW8
NB-ARC PF00931 165 - 321 5e-15 NB-ARC domain
AAA_22 PF13401 182 - 270 1.2e-06 AAA domain
LRR_14 PF23598 663 - 774 3.4e-16 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000039)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66890 AT5G66900 AT5G66900 AT5G66900 AT5G66910
fragaria_vesca FvH4_1g15090 FvH4_1g15120 FvH4_1g15130 FvH4_1g15140 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15170 FvH4_1g15171 FvH4_1g15171 FvH4_1g15200 FvH4_1g15200 FvH4_1g15200 FvH4_1g15210 FvH4_1g15210 FvH4_1g15210 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15230 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15500 FvH4_1g15500 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_2g19760 FvH4_3g12820 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_6g18970 FvH4_6g42370 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g50100 FvH4_6g50100 FvH4_6g50110 FvH4_6g50130 FvH4_6g50140 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50330
malus_domestica MD00G1053700.v1.1 MD02G1164300.v1.1 MD02G1164400.v1.1 MD02G1164700.v1.1 MD02G1164800.v1.1 MD02G1165300.v1.1 MD02G1165400.v1.1 MD02G1165500.v1.1 MD02G1165600.v1.1 MD02G1165900.v1.1 MD02G1166000.v1.1 MD02G1166100.v1.1 MD02G1166300.v1.1 MD08G1084900.v1.1 MD15G1263100.v1.1 MD15G1263300.v1.1 MD15G1276900.v1.1 MD15G1277200.v1.1 MD15G1277400.v1.1 MD15G1277700.v1.1 MD15G1277800.v1.1 MD15G1278000.v1.1 MD15G1278100.v1.1 MD15G1278400.v1.1 MD15G1278600.v1.1
prunus_persica Prupe.7G138500_v2.0.a1 Prupe.7G138500_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138700_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138900_v2.0.a1 Prupe.7G139100_v2.0.a1 Prupe.7G139200_v2.0.a1 Prupe.7G139500_v2.0.a1 Prupe.7G139600_v2.0.a1 Prupe.7G139700_v2.0.a1 Prupe.7G139800_v2.0.a1
pyrus_communis pycom02g13150 pycom02g13160 pycom02g13190 pycom02g13200 pycom02g13220 pycom02g13230 pycom02g13250 pycom02g13260 pycom02g13270 pycom02g13300 pycom02g13310 pycom02g13330 pycom15g23980 pycom15g23990 pycom15g24030 pycom15g24040 pycom15g24050 pycom15g24070 pycom15g24090 pycom15g24160
rosa_chinensis RchiOBHm_Chr1g0326431 RchiOBHm_Chr1g0326451 RchiOBHm_Chr2g0104121 RchiOBHm_Chr2g0104151 RchiOBHm_Chr2g0104171 RchiOBHm_Chr2g0104181 RchiOBHm_Chr2g0104191 RchiOBHm_Chr2g0104201 RchiOBHm_Chr2g0104211 RchiOBHm_Chr2g0104241 RchiOBHm_Chr2g0104261 RchiOBHm_Chr2g0104281 RchiOBHm_Chr2g0104311 RchiOBHm_Chr2g0104331 RchiOBHm_Chr2g0104361 RchiOBHm_Chr2g0104381 RchiOBHm_Chr2g0104391 RchiOBHm_Chr2g0104431 RchiOBHm_Chr2g0104441 RchiOBHm_Chr2g0104541 RchiOBHm_Chr2g0104611 RchiOBHm_Chr2g0104741 RchiOBHm_Chr2g0104761 RchiOBHm_Chr2g0158291 RchiOBHm_Chr2g0170371 RchiOBHm_Chr2g0170631 RchiOBHm_Chr3g0473971 RchiOBHm_Chr3g0480491 RchiOBHm_Chr3g0480541 RchiOBHm_Chr3g0480561 RchiOBHm_Chr3g0485281 RchiOBHm_Chr3g0485291 RchiOBHm_Chr4g0411541 RchiOBHm_Chr6g0285471
rosa_laevigata RLG00000008347 RLG00000012637 RLG00000016961 RLG00000017293 RLG00000017305 RLG00000017308 RLG00000017312 RLG00000017314 RLG00000017317 RLG00000017318 RLG00000017319 RLG00000017321 RLG00000017322 RLG00000017323 RLG00000017324 RLG00000017325 RLG00000017327 RLG00000017328 RLG00000017329 RLG00000017330 RLG00000017334 RLG00000020833 RLG00000021073 RLG00000021950 RLG00000021971 RLG00000023154 RLG00000023157 RLG00000023467 RLG00000023468 RLG00000023525 RLG00000023963 RLG00000033265 RLG00000033267 RLG00000033268
rosa_multiflora Rmu_co8053082.1_g000001 Rmu_co8210358.1_g000001 Rmu_co8331661.1_g000001 Rmu_co8477427.1_g000002 Rmu_co8483217.1_g000001 Rmu_sc0000284.1_g000004 Rmu_sc0000676.1_g000017 Rmu_sc0000676.1_g000019 Rmu_sc0000676.1_g000021 Rmu_sc0002205.1_g000038 Rmu_sc0002205.1_g000041 Rmu_sc0002316.1_g000010 Rmu_sc0002316.1_g000012 Rmu_sc0002316.1_g000040 Rmu_sc0002316.1_g000071 Rmu_sc0002316.1_g000072 Rmu_sc0002316.1_g000095 Rmu_sc0002316.1_g000102 Rmu_sc0002414.1_g000018 Rmu_sc0002576.1_g000002 Rmu_sc0002576.1_g000003 Rmu_sc0002687.1_g000010 Rmu_sc0002687.1_g000017 Rmu_sc0003660.1_g000001 Rmu_sc0003787.1_g000014 Rmu_sc0003787.1_g000020 Rmu_sc0003787.1_g000024 Rmu_sc0003787.1_g000029 Rmu_sc0004059.1_g000026 Rmu_sc0005137.1_g000021 Rmu_sc0006550.1_g000001 Rmu_sc0006561.1_g000025 Rmu_sc0006561.1_g000033 Rmu_sc0007704.1_g000005 Rmu_sc0008904.1_g000005 Rmu_sc0010270.1_g000002 Rmu_sc0010307.1_g000001 Rmu_sc0011601.1_g000013 Rmu_sc0012694.1_g000005 Rmu_sc0013101.1_g000008 Rmu_sc0019029.1_g000001 Rmu_sc0019728.1_g000001 Rmu_sc0025829.1_g000001 Rmu_sc0025829.1_g000002 Rmu_ssc0000257.1_g000007 Rmu_ssc0000257.1_g000008
rosa_roxburghii Rroxscaffold_1G00021710 Rroxscaffold_1G00049350 Rroxscaffold_1G00049360 Rroxscaffold_2G00081160 Rroxscaffold_2G00081360 Rroxscaffold_2G00081390 Rroxscaffold_2G00091540 Rroxscaffold_2G00138790 Rroxscaffold_2G00138800 Rroxscaffold_2G00138920 Rroxscaffold_2G00139000 Rroxscaffold_2G00139010 Rroxscaffold_2G00139020 Rroxscaffold_2G00139030 Rroxscaffold_2G00139040 Rroxscaffold_2G00139050 Rroxscaffold_2G00139060 Rroxscaffold_2G00139070 Rroxscaffold_2G00139090 Rroxscaffold_2G00139100 Rroxscaffold_3G00257470 Rroxscaffold_4G00318040 Rroxscaffold_4G00323740 Rroxscaffold_4G00323750 Rroxscaffold_5G00356140 Rroxscaffold_6G00401280 Rroxscaffold_6G00401290 Rroxscaffold_7G00183120
rosa_rugosa Rorug01G0060500 Rorug01G0060900 Rorug02G0119500 Rorug02G0119600 Rorug02G0119700 Rorug02G0119700 Rorug02G0119700 Rorug02G0119800.1 Rorug02G0119900.1 Rorug02G0120000 Rorug02G0120100 Rorug02G0120200 Rorug02G0120300 Rorug02G0120400 Rorug02G0120500 Rorug02G0120700 Rorug02G0120700 Rorug02G0122300 Rorug02G0122300 Rorug02G0122400.1 Rorug02G0466700 Rorug02G0466700 Rorug02G0466800 Rorug02G0548600 Rorug02G0549900 Rorug02G0550000 Rorug03G0184200 Rorug04G0107900 Rorug05G0121500 Rorug06G0174800
rosa_samantha Rh1AG198400 Rh1CG074700 Rh1CG075000 Rh1CG183700 Rh2AG169700 Rh2AG169800 Rh2AG169900 Rh2AG170000 Rh2AG170300 Rh2AG170400 Rh2AG171000 Rh2AG171100 Rh2AG171600 Rh2AG172400 Rh2AG172500 Rh2AG532900 Rh2AG620000 Rh2AG622500 Rh2AG622800 Rh2AG623000 Rh2BG176200 Rh2BG176300 Rh2BG176400 Rh2BG176500 Rh2BG176600 Rh2BG176900 Rh2BG177000 Rh2BG177100 Rh2BG177200 Rh2BG177300 Rh2BG177600 Rh2BG177700 Rh2BG177900 Rh2BG178000 Rh2BG178200 Rh2BG178300 Rh2BG178500 Rh2BG179000 Rh2BG180000 Rh2BG180100 Rh2BG259200 Rh2BG546700 Rh2BG630800 Rh2BG633500 Rh2CG176500 Rh2CG177500 Rh2CG177700 Rh2DG174400 Rh2DG174600 Rh2DG174900 Rh2DG175100 Rh2DG175200 Rh2DG175300 Rh2DG175600 Rh2DG175700 Rh2DG175800 Rh2DG175900 Rh2DG176000 Rh2DG176300 Rh2DG176400 Rh2DG176500 Rh2DG176600 Rh2DG176900 Rh2DG177100 Rh2DG177200 Rh2DG177800 Rh2DG177900 Rh2DG178700 Rh2DG178800 Rh2DG555700 Rh2DG643700 Rh2DG646300 Rh3AG235600 Rh3AG235900 Rh3AG236200 Rh3AG266700 Rh3AG266900 Rh3BG216200 Rh3BG269100 Rh3CG212700 Rh3CG265800 Rh4AG168900 Rh4BG165500 Rh4CG180300 Rh5BG261500 Rh6AG286300 Rh6BG287500 Rh6CG288600 Rh7AG022600
rosa_wichuraiana Rw0G008240 Rw0G008250 Rw0G013190 Rw0G013200 Rw2G013230 Rw2G013240 Rw2G013270 Rw2G013280 Rw2G013290 Rw2G013300 Rw2G013330 Rw2G013340 Rw2G013350 Rw2G013360 Rw2G013370 Rw2G013380 Rw2G013410 Rw2G013570 Rw2G044120 Rw2G051370 Rw2G051590 Rw3G017160 Rw3G021150 Rw3G021160 Rw3G023730 Rw3G023740 Rw4G014030 Rw6G024590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 71, 489
Acc36I ACCTGC 2 cut(s) 352, 2394
AccB1I GGYRCC 1 cut(s) 133
AccB7I CCANNNNNTGG 3 cut(s) 172, 905, 1545
AccII CGCG 5 cut(s) 259, 1697, 1877, 1889, 2391
AciI CCGC 5 cut(s) 423, 524, 1695, 1889, 2203
AclWI GGATC 5 cut(s) 380, 826, 1246, 2045, 2310
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 8 cut(s) 79, 623, 893, 901, 1759, 1936, 1982, 2089
AcuI CTGAAG 1 cut(s) 1269
AcyI GRCGYC 1 cut(s) 108
AfaI GTAC 7 cut(s) 281, 287, 442, 738, 1704, 1728, 2109
AfeI AGCGCT 1 cut(s) 989
AfiI CCNNNNNNNGG 8 cut(s) 117, 172, 187, 310, 905, 1545, 1573, 2392
AflIII ACRYGT 1 cut(s) 1284
AjnI CCWGG 4 cut(s) 116, 290, 559, 1233
AjuI GAANNNNNNNTTGG 2 cut(s) 892, 924
Alw21I GWGCWC 3 cut(s) 234, 248, 552
Alw26I GTCTC 4 cut(s) 99, 1145, 1175, 1817
AlwI GGATC 5 cut(s) 380, 826, 1246, 2045, 2310
Aor51HI AGCGCT 1 cut(s) 989
AoxI GGCC 4 cut(s) 3, 223, 1622, 2060
ApeKI GCWGC 4 cut(s) 1179, 1998, 2284, 2451
ApoI RAATTY 8 cut(s) 79, 623, 893, 901, 1759, 1936, 1982, 2089
AseI ATTAAT 1 cut(s) 1754
Asp700I GAANNNNTTC 5 cut(s) 35, 831, 1161, 1983, 2364
AspLEI GCGC 4 cut(s) 157, 990, 1889, 2391
AspS9I GGNCC 5 cut(s) 491, 796, 1231, 1623, 2060
AsuC2I CCSGG 1 cut(s) 1487
AsuHPI GGTGA 5 cut(s) 1426, 1433, 1556, 2240, 2410
AsuII TTCGAA 1 cut(s) 39
AvaII GGWCC 3 cut(s) 491, 796, 1231
BanI GGYRCC 1 cut(s) 133
BanII GRGCYC 3 cut(s) 234, 269, 1676
BarI GAAGNNNNNNTAC 2 cut(s) 729, 761
BbsI GAAGAC 3 cut(s) 69, 781, 1257
Bbv12I GWGCWC 3 cut(s) 234, 248, 552
BbvI GCAGC 3 cut(s) 1191, 1985, 2271
BccI CCATC 6 cut(s) 145, 255, 482, 1393, 2162, 2299
BceAI ACGGC 2 cut(s) 485, 1756
BcgI CGANNNNNNTGC 2 cut(s) 529, 563
BciT130I CCWGG 4 cut(s) 118, 292, 561, 1235
BciVI GTATCC 1 cut(s) 2333
BclI TGATCA 1 cut(s) 141
BcnI CCSGG 1 cut(s) 1487
BcoDI GTCTC 4 cut(s) 99, 1145, 1175, 1817
BfaI CTAG 4 cut(s) 26, 1142, 1481, 2455
BfmI CTRYAG 1 cut(s) 1089
BfoI RGCGCY 1 cut(s) 991
BfuAI ACCTGC 2 cut(s) 352, 2394
BfuI GTATCC 1 cut(s) 2333
BglII AGATCT 1 cut(s) 887
BisI GCNGC 4 cut(s) 1180, 1999, 2285, 2452
BlpI GCTNAGC 1 cut(s) 1476
BlsI GCNGC 4 cut(s) 1181, 2000, 2286, 2453
BmcAI AGTACT 1 cut(s) 2109
Bme1390I CCNGG 5 cut(s) 118, 292, 561, 1235, 1487
Bme18I GGWCC 3 cut(s) 491, 796, 1231
BmgT120I GGNCC 5 cut(s) 491, 796, 1231, 1623, 2060
BmiI GGNNCC 4 cut(s) 135, 266, 717, 2361
BmrFI CCNGG 5 cut(s) 118, 292, 561, 1235, 1487
BmrI ACTGGG 1 cut(s) 2142
BmsI GCATC 4 cut(s) 396, 744, 1177, 2186
BmuI ACTGGG 1 cut(s) 2142
BpiI GAAGAC 3 cut(s) 69, 781, 1257
BplI GAGNNNNNCTC 2 cut(s) 993, 1025
BpmI CTGGAG 2 cut(s) 582, 1120
Bpu10I CCTNAGC 1 cut(s) 2117
Bpu1102I GCTNAGC 1 cut(s) 1476
Bpu14I TTCGAA 1 cut(s) 39
BpuEI CTTGAG 3 cut(s) 326, 868, 997
BpuMI CCSGG 1 cut(s) 1487
Bsa29I ATCGAT 1 cut(s) 375
BsaBI GATNNNNATC 3 cut(s) 396, 2027, 2307
BsaHI GRCGYC 1 cut(s) 108
BsaI GGTCTC 1 cut(s) 1817
BsaJI CCNNGG 5 cut(s) 6, 467, 1234, 2335, 2344
BsaWI WCCGGW 1 cut(s) 712
Bsc4I CCNNNNNNNGG 8 cut(s) 117, 172, 187, 310, 905, 1545, 1573, 2392
Bse1I ACTGG 4 cut(s) 198, 489, 1734, 2148
Bse8I GATNNNNATC 3 cut(s) 396, 2027, 2307
BseBI CCWGG 4 cut(s) 118, 292, 561, 1235
BseCI ATCGAT 1 cut(s) 375
BseDI CCNNGG 5 cut(s) 6, 467, 1234, 2335, 2344
BseGI GGATG 6 cut(s) 493, 532, 759, 940, 988, 2201
BseJI GATNNNNATC 3 cut(s) 396, 2027, 2307
BseLI CCNNNNNNNGG 8 cut(s) 117, 172, 187, 310, 905, 1545, 1573, 2392
BseMII CTCAG 1 cut(s) 2102
BseNI ACTGG 4 cut(s) 198, 489, 1734, 2148
BseRI GAGGAG 4 cut(s) 2023, 2026, 2029, 2275
BseXI GCAGC 3 cut(s) 1191, 1985, 2271
Bsh1236I CGCG 5 cut(s) 259, 1697, 1877, 1889, 2391
BshFI GGCC 4 cut(s) 5, 225, 1624, 2062
BshNI GGYRCC 1 cut(s) 133
BshVI ATCGAT 1 cut(s) 375
BsiHKAI GWGCWC 3 cut(s) 234, 248, 552
BsiSI CCGG 2 cut(s) 713, 1487
BslFI GGGAC 1 cut(s) 1421
BslI CCNNNNNNNGG 8 cut(s) 117, 172, 187, 310, 905, 1545, 1573, 2392
BsmAI GTCTC 4 cut(s) 99, 1145, 1175, 1817
BsmBI CGTCTC 1 cut(s) 1145
BsmFI GGGAC 1 cut(s) 1421
BsmI GAATGC 1 cut(s) 35
BsnI GGCC 4 cut(s) 5, 225, 1624, 2062
Bso31I GGTCTC 1 cut(s) 1817
Bsp119I TTCGAA 1 cut(s) 39
Bsp1286I GDGCHC 5 cut(s) 234, 248, 269, 552, 1676
Bsp1407I TGTACA 1 cut(s) 1702
Bsp1720I GCTNAGC 1 cut(s) 1476
BspACI CCGC 5 cut(s) 423, 524, 1695, 1889, 2203
BspANI GGCC 4 cut(s) 5, 225, 1624, 2062
BspCNI CTCAG 1 cut(s) 2103
BspDI ATCGAT 1 cut(s) 375
BspFNI CGCG 5 cut(s) 259, 1697, 1877, 1889, 2391
BspLI GGNNCC 4 cut(s) 135, 266, 717, 2361
BspMI ACCTGC 2 cut(s) 352, 2394
BspPI GGATC 5 cut(s) 380, 826, 1246, 2045, 2310
BspT104I TTCGAA 1 cut(s) 39
BspT107I GGYRCC 1 cut(s) 133
BspTNI GGTCTC 1 cut(s) 1817
BsrGI TGTACA 1 cut(s) 1702
BsrI ACTGG 4 cut(s) 198, 489, 1734, 2148
BssECI CCNNGG 5 cut(s) 6, 467, 1234, 2335, 2344
BssNI GRCGYC 1 cut(s) 108
BssT1I CCWWGG 1 cut(s) 2344
Bst2UI CCWGG 4 cut(s) 118, 292, 561, 1235
Bst4CI ACNGT 8 cut(s) 330, 652, 1060, 1319, 1421, 1428, 2336, 2444
Bst6I CTCTTC 2 cut(s) 104, 603
BstACI GRCGYC 1 cut(s) 108
BstAUI TGTACA 1 cut(s) 1702
BstBI TTCGAA 1 cut(s) 39
BstC8I GCNNGC 3 cut(s) 1579, 1691, 1695
BstDEI CTNAG 6 cut(s) 1183, 1451, 1476, 2101, 2111, 2117
BstDSI CCRYGG 3 cut(s) 6, 467, 2335
BstF5I GGATG 6 cut(s) 493, 532, 759, 940, 988, 2201
BstFNI CGCG 5 cut(s) 259, 1697, 1877, 1889, 2391
BstH2I RGCGCY 1 cut(s) 991
BstHHI GCGC 4 cut(s) 157, 990, 1889, 2391
BstMAI GTCTC 4 cut(s) 99, 1145, 1175, 1817
BstMWI GCNNNNNNNGC 2 cut(s) 41, 1630
BstNI CCWGG 4 cut(s) 118, 292, 561, 1235
BstNSI RCATGY 1 cut(s) 1288
BstSCI CCNGG 5 cut(s) 116, 290, 559, 1233, 1485
BstSFI CTRYAG 1 cut(s) 1089
BstUI CGCG 5 cut(s) 259, 1697, 1877, 1889, 2391
BstV1I GCAGC 3 cut(s) 1191, 1985, 2271
BstV2I GAAGAC 3 cut(s) 69, 781, 1257
BstX2I RGATCY 3 cut(s) 887, 1238, 2302
BstYI RGATCY 3 cut(s) 887, 1238, 2302
Bsu15I ATCGAT 1 cut(s) 375
BsuI GTATCC 1 cut(s) 2333
BsuRI GGCC 4 cut(s) 5, 225, 1624, 2062
BsuTUI ATCGAT 1 cut(s) 375
BtgI CCRYGG 3 cut(s) 6, 467, 2335
BtgZI GCGATG 1 cut(s) 274
BtsCI GGATG 6 cut(s) 493, 532, 759, 940, 988, 2201
BtsI GCAGTG 2 cut(s) 51, 552
BtsIMutI CAGTG 5 cut(s) 51, 137, 552, 1065, 1324
BveI ACCTGC 2 cut(s) 352, 2394
Cac8I GCNNGC 3 cut(s) 1579, 1691, 1695
CfoI GCGC 4 cut(s) 157, 990, 1889, 2391
Cfr13I GGNCC 5 cut(s) 491, 796, 1231, 1623, 2060
ClaI ATCGAT 1 cut(s) 375
CseI GACGC 2 cut(s) 116, 1866
Csp6I GTAC 7 cut(s) 280, 286, 441, 737, 1703, 1727, 2108
CspCI CAANNNNNGTGG 2 cut(s) 2052, 2087
CviAII CATG 5 cut(s) 214, 1285, 1442, 1881, 2257
CviQI GTAC 7 cut(s) 280, 286, 441, 737, 1703, 1727, 2108
DdeI CTNAG 6 cut(s) 1183, 1451, 1476, 2101, 2111, 2117
DraI TTTAAA 3 cut(s) 204, 924, 2016
DrdI GACNNNNNNGTC 2 cut(s) 71, 489
DseDI GACNNNNNNGTC 2 cut(s) 71, 489
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 104, 603
EarI CTCTTC 2 cut(s) 104, 603
Ecl136II GAGCTC 1 cut(s) 232
Eco130I CCWWGG 1 cut(s) 2344
Eco24I GRGCYC 3 cut(s) 234, 269, 1676
Eco31I GGTCTC 1 cut(s) 1817
Eco47I GGWCC 3 cut(s) 491, 796, 1231
Eco47III AGCGCT 1 cut(s) 989
Eco53kI GAGCTC 1 cut(s) 232
Eco57I CTGAAG 1 cut(s) 1269
EcoICRI GAGCTC 1 cut(s) 232
EcoRII CCWGG 4 cut(s) 116, 290, 559, 1233
EcoT14I CCWWGG 1 cut(s) 2344
EcoT38I GRGCYC 3 cut(s) 234, 269, 1676
ErhI CCWWGG 1 cut(s) 2344
Esp3I CGTCTC 1 cut(s) 1145
FaeI CATG 5 cut(s) 217, 1288, 1445, 1884, 2260
FalI AAGNNNNNCTT 8 cut(s) 83, 115, 1565, 1597, 1971, 2003, 2162, 2194
FaqI GGGAC 1 cut(s) 1421
FatI CATG 5 cut(s) 213, 1284, 1441, 1880, 2256
FauI CCCGC 2 cut(s) 430, 1702
FauNDI CATATG 2 cut(s) 741, 1392
FbaI TGATCA 1 cut(s) 141
Fnu4HI GCNGC 4 cut(s) 1180, 1999, 2285, 2452
FokI GGATG 6 cut(s) 500, 539, 766, 947, 995, 2208
FriOI GRGCYC 3 cut(s) 234, 269, 1676
Fsp4HI GCNGC 4 cut(s) 1180, 1999, 2285, 2452
FspBI CTAG 4 cut(s) 26, 1142, 1481, 2455
GlaI GCGC 4 cut(s) 156, 989, 1888, 2390
GluI GCNGC 4 cut(s) 1180, 1999, 2285, 2452
GsuI CTGGAG 2 cut(s) 582, 1120
HaeII RGCGCY 1 cut(s) 991
HaeIII GGCC 4 cut(s) 5, 225, 1624, 2062
HapII CCGG 2 cut(s) 713, 1487
HgaI GACGC 2 cut(s) 116, 1866
HhaI GCGC 4 cut(s) 157, 990, 1889, 2391
Hin1I GRCGYC 1 cut(s) 108
Hin1II CATG 5 cut(s) 217, 1288, 1445, 1884, 2260
Hin6I GCGC 4 cut(s) 155, 988, 1887, 2389
HinP1I GCGC 4 cut(s) 155, 988, 1887, 2389
HincII GTYRAC 3 cut(s) 238, 1375, 2098
HindII GTYRAC 3 cut(s) 238, 1375, 2098
HindIII AAGCTT 3 cut(s) 198, 515, 2141
HpaII CCGG 2 cut(s) 713, 1487
HphI GGTGA 5 cut(s) 1426, 1433, 1556, 2240, 2410
Hpy166II GTNNAC 6 cut(s) 238, 443, 1375, 1424, 2098, 2278
Hpy8I GTNNAC 6 cut(s) 238, 443, 1375, 1424, 2098, 2278
Hpy99I CGWCG 3 cut(s) 110, 1904, 2444
HpyAV CCTTC 4 cut(s) 181, 280, 1717, 1898
HpyCH4III ACNGT 8 cut(s) 330, 652, 1060, 1319, 1421, 1428, 2336, 2444
HpyCH4IV ACGT 1 cut(s) 2065
HpyF10VI GCNNNNNNNGC 2 cut(s) 41, 1630
HpyF3I CTNAG 6 cut(s) 1183, 1451, 1476, 2101, 2111, 2117
HpySE526I ACGT 1 cut(s) 2065
Hsp92I GRCGYC 1 cut(s) 108
Hsp92II CATG 5 cut(s) 217, 1288, 1445, 1884, 2260
HspAI GCGC 4 cut(s) 155, 988, 1887, 2389
Ksp22I TGATCA 1 cut(s) 141
LmnI GCTCC 3 cut(s) 264, 562, 2448
Lsp1109I GCAGC 3 cut(s) 1191, 1985, 2271
LweI GCATC 4 cut(s) 396, 744, 1177, 2186
MaeI CTAG 4 cut(s) 26, 1142, 1481, 2455
MaeII ACGT 1 cut(s) 2065
MaeIII GTNAC 7 cut(s) 877, 1223, 1432, 1562, 1682, 1779, 2066
MfeI CAATTG 1 cut(s) 1209
MflI RGATCY 3 cut(s) 887, 1238, 2302
MhlI GDGCHC 5 cut(s) 234, 248, 269, 552, 1676
MlyI GAGTC 2 cut(s) 219, 441
MmeI TCCRAC 6 cut(s) 312, 460, 1045, 2232, 2406, 2426
MroXI GAANNNNTTC 5 cut(s) 35, 831, 1161, 1983, 2364
MseI TTAA 9 cut(s) 90, 203, 321, 923, 1754, 1949, 2015, 2174, 2243
MslI CAYNNNNRTG 1 cut(s) 1283
MspI CCGG 2 cut(s) 713, 1487
MspR9I CCNGG 5 cut(s) 118, 292, 561, 1235, 1487
MunI CAATTG 1 cut(s) 1209
Mva1269I GAATGC 1 cut(s) 35
MvaI CCWGG 4 cut(s) 118, 292, 561, 1235
MvnI CGCG 5 cut(s) 259, 1697, 1877, 1889, 2391
MwoI GCNNNNNNNGC 2 cut(s) 41, 1630
NciI CCSGG 1 cut(s) 1487
NdeI CATATG 2 cut(s) 741, 1392
NlaIII CATG 5 cut(s) 217, 1288, 1445, 1884, 2260
NlaIV GGNNCC 4 cut(s) 135, 266, 717, 2361
NmuCI GTSAC 2 cut(s) 1432, 1562
NspI RCATGY 1 cut(s) 1288
NspV TTCGAA 1 cut(s) 39
PciI ACATGT 1 cut(s) 1284
PctI GAATGC 1 cut(s) 35
PdmI GAANNNNTTC 5 cut(s) 35, 831, 1161, 1983, 2364
PfeI GAWTC 9 cut(s) 314, 353, 698, 827, 1130, 1357, 1797, 2008, 2401
PflMI CCANNNNNTGG 3 cut(s) 172, 905, 1545
PfoI TCCNGGA 1 cut(s) 559
PkrI GCNGC 4 cut(s) 1181, 2000, 2286, 2453
PleI GAGTC 2 cut(s) 218, 441
PpsI GAGTC 2 cut(s) 218, 441
PscI ACATGT 1 cut(s) 1284
PshBI ATTAAT 1 cut(s) 1754
Psp124BI GAGCTC 1 cut(s) 234
Psp6I CCWGG 4 cut(s) 116, 290, 559, 1233
PspGI CCWGG 4 cut(s) 116, 290, 559, 1233
PspN4I GGNNCC 4 cut(s) 135, 266, 717, 2361
PspPI GGNCC 5 cut(s) 491, 796, 1231, 1623, 2060
PsuI RGATCY 3 cut(s) 887, 1238, 2302
RsaI GTAC 7 cut(s) 281, 287, 442, 738, 1704, 1728, 2109
RsaNI GTAC 7 cut(s) 280, 286, 441, 737, 1703, 1727, 2108
RseI CAYNNNNRTG 1 cut(s) 1283
SacI GAGCTC 1 cut(s) 234
SaqAI TTAA 9 cut(s) 90, 203, 321, 923, 1754, 1949, 2015, 2174, 2243
SatI GCNGC 4 cut(s) 1180, 1999, 2285, 2452
Sau96I GGNCC 5 cut(s) 491, 796, 1231, 1623, 2060
ScaI AGTACT 1 cut(s) 2109
SchI GAGTC 2 cut(s) 219, 441
ScrFI CCNGG 5 cut(s) 118, 292, 561, 1235, 1487
SduI GDGCHC 5 cut(s) 234, 248, 269, 552, 1676
SfaNI GCATC 4 cut(s) 396, 744, 1177, 2186
SfcI CTRYAG 1 cut(s) 1089
SfuI TTCGAA 1 cut(s) 39
SinI GGWCC 3 cut(s) 491, 796, 1231
SmiMI CAYNNNNRTG 1 cut(s) 1283
SmlI CTYRAG 3 cut(s) 341, 883, 1012
SmoI CTYRAG 3 cut(s) 341, 883, 1012
SsiI CCGC 5 cut(s) 423, 524, 1695, 1889, 2203
SspI AATATT 1 cut(s) 637
SspMI CTAG 4 cut(s) 26, 1142, 1481, 2455
SstI GAGCTC 1 cut(s) 234
StyD4I CCNGG 5 cut(s) 116, 290, 559, 1233, 1485
StyI CCWWGG 1 cut(s) 2344
TaaI ACNGT 8 cut(s) 330, 652, 1060, 1319, 1421, 1428, 2336, 2444
TaiI ACGT 1 cut(s) 2068
TatI WGTACW 5 cut(s) 279, 440, 736, 1702, 2107
TfiI GAWTC 9 cut(s) 314, 353, 698, 827, 1130, 1357, 1797, 2008, 2401
Tru1I TTAA 9 cut(s) 90, 203, 321, 923, 1754, 1949, 2015, 2174, 2243
Tru9I TTAA 9 cut(s) 90, 203, 321, 923, 1754, 1949, 2015, 2174, 2243
TscAI CASTG 5 cut(s) 51, 144, 559, 1065, 1324
TseFI GTSAC 2 cut(s) 1432, 1562
TseI GCWGC 4 cut(s) 1179, 1998, 2284, 2451
Tsp45I GTSAC 2 cut(s) 1432, 1562
TspGWI ACGGA 4 cut(s) 691, 1053, 1169, 2043
TspRI CASTG 5 cut(s) 51, 144, 559, 1065, 1324
Van91I CCANNNNNTGG 3 cut(s) 172, 905, 1545
VpaK11BI GGWCC 3 cut(s) 491, 796, 1231
VspI ATTAAT 1 cut(s) 1754
XapI RAATTY 8 cut(s) 79, 623, 893, 901, 1759, 1936, 1982, 2089
XbaI TCTAGA 2 cut(s) 25, 1141
XceI RCATGY 1 cut(s) 1288
XcmI CCANNNNNNNNNTGG 1 cut(s) 1357
XmnI GAANNNNTTC 5 cut(s) 35, 831, 1161, 1983, 2364
XspI CTAG 4 cut(s) 26, 1142, 1481, 2455
ZrmI AGTACT 1 cut(s) 2109
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.