FvH4_1g15120

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0000039
Physical Location & Seq
Forward (+)
8563308 .. 8567607
4300 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g15120.t1

Sequence Viewer

Length: 2730 bp
ATGAAGAACAAGCAAACAGCGTGGTCAATGGCCTCCAACTTGGAGCTTGCTGGGTCAATCAATGGACTCGCAGTTGAAACTTCGGAGCTTCCCATTGAACGCGTCCCCCTTTACCAAGCTTTTATTGGAATCTTTGCATCACTGGACTTCACCAATTACAAATTTGATCACCAACACAGAGGATCAAGAATGGCGGACCTCGCTGCAGGTGGTGCTATGGGATTGCCATTTACTGTACTGTACGAAGTCATTAAGGAAGTGGTGATCAAGACTGCGATGTTTGAACCTCTCCTCAAAGAACTTGATTTTACAATAGACTCTTTGAAACCACTGATCGAGGAAATGGACAAGTACAATAAGGTGTTGGATCGTCCAGAGAAGGAACTTCGAGAATTTAAAGTACAAATGGAGAAGGGCCCTGAGCTTATTCGCAAGTGCGCCAATCTTAGCAAGTGGCAAAGCTACAAAAAGTACAAGTACAGCAACCAACTTCTTGAATTGCAGAACTCTCTTCAGAAGCTGCAATTTCTACTGTCGGTACAACAAGCAAGGGATGTGAAGCAGACCTTGGCTGTAACAAAGAATATAGAGACTGTGGTGCTGCGAATAGATGGTGAGGGTCCTGCAGGATGGAAAGAAGGGATGAATGATATGAAAGAGACCTTGGAAGAAGTGAAGATTATAACAGGGACGATGGTCAAGCGCATTGATCAGAAAGGTGAGGTACAAAGTCAAATAGACAGTGAAGTACCTGCATCTCCAAGGGTTACTGTGGGATTGGATGTGCCTTTGATGGAATTGAAGATGAAGCTTCTTAAGGACGATGAGGTAACAATGCTAGTGCTGACTGCTCCTGGAGGATCCGGGAAAACCACATTGGCCACAAAGTTTTGTCAAGATAAGGATGTCCAAGGTAAATTCAAGAACAATATCTTCTTTTTCACGGTTTCAAAGAAGTCGAACTTCAACCTTATTGTACAAGGGCTGCATCAACGCAAGAGTACTGATGTACCTTCTTTCCCAGATGAAGCAACTGCCGTCAAATGGCTGCATAAGTTTCTGAAGGAAGAAGATGTGCCTGTACTGTTGGTCTTGGATGATGTTTGGTCTGAATCAAAATCTATTCTTGAAACATTTGATGAAGTGAAAATGCCAAATTTCAAGATTTTGGTGACATCAAGATCACAGTTTCCAGGATATGGTTCTCTGTATCATCTGCAATCACTAAATGATATAGATGCAATGAAACTTTTGCGTCATTCAGCATCCCTAGGCGATGGGAGCTTTAATTTTCAATGGAACAGACTTGCTAAAAAGATAGTAGAACACTGCAAGGGATATCCACTTGCTATTGATGTGGTCGGAAAATCACTCCGAGGAAAATCTTATGAGTTATGGTGTAAAAGAGAAATGGAGTTGTCCAAAAGTGATTCTATTATTGATTCTGAAACTGAAGTGCTTCTTCGCCTTAAAAGCAGCTTAGATGCTTTGAATGAACAAGAAGCAATCATCAAGGAATGTTTTGTAGACCTTGGTTCATTTCCTGAAGGCCAAAGAATCCCTGTGGTTGCTCTCATTGACATGTGGGCAGAGTTGCATGAAGGGCTAGGGAAAGATGTTTTGGCCGTTGCAAACCTGCAAGAGCTTACTTCTCAAAGTCTGGCTGATCTTGTAGTCACAAGGAAGGAGATAATGGAGGTTGATGACTACTACAGTGAGCACTTCGTCACCCAACATGATTTGCTCAGACAGCTGGCTGTTTATGAAGCCAAACTAGACTCAAGTAAAAAGCGATTGATCATAGGTAACTGCGGAGACAATCTACCCAAGTGCTTGACTGAACAGAAGGATCAACTGATTAAGCCTCGTCTGCTATCTATCTCCTCAGATGAAACGTTCTCAACAAATTTACACAACATTCATCTAGCAGCTGTTGAGGTTCTAGTTTTGAATTTCAATGCAAAGAATTATGCTTTGCCTGAATTTGTGGAGAAAATGATTAATCTGAAGGTTCTAATAATCACAAATCATGGTTTCTTACCTGCTGAATTGAGCAATTTTCACCTCCTGGATTCTTTACCAAATCTTAAGAGAATCAGATTAGAGCGTGTTTCAATTCCTTCCATAACCAAGAATTCGATACAGTTGAAGAGCCTGAAGAAGATATCTTTATTCATGTGTAGCATCGGTCAAGCTTTCAGCAATTGTTCCTTTGACATTTCAGATGCATTGCCAAATTTGGAGGAACTAAATATTGATTATTGCAATGACTTTGTGGGATTGCCTGACACGATCTGCAATTTGGTTCGTCTAAGGAAGCTCAGTATCACAAATTGTCATAAGCTATCTGCCTTGCCTAAAGAGATTGGAAAGCTGGATATGCTAGAGGTTTTGAGGCTAAGGTCTTGTACAGATTTGGTAAAACTTCCGTGCTCTGTTATGAACCTGGGGAGTTTAAGCTTTCTTGACATATCTGACTGTTTCAGCATTGTGGAGTTGCCTGAAGACATTGGTGATCTCAGCGGTTTAAGAAAGATCAACATGAGTCAGTGCTCTAGATTGAAAGAGCTACCACTATCAGTTTTGGATTTTGAGAATATAGAGGAGGTCATATGCGATGAAGAGACAAAAGAGTTGTGGGAGCCCTTCTTGCCTATGCTCCACAGCGTAGCAGTAAGGGTGGTCAAACAAGAATTCAACCTAAATTGGCTTCAAAAGTCCCAGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

910

Amino Acids

102.98

Weight (kDa)

5.67

Isoelectric Point (pI)

39.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 65 - 198 3.4e-31 Arabidopsis broad-spectrum mildew resistance protein RPW8
NB-ARC PF00931 265 - 396 9.7e-16 NB-ARC domain
LRR_14 PF23598 764 - 826 2.1e-10 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000039)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66890 AT5G66900 AT5G66900 AT5G66900 AT5G66910
fragaria_vesca FvH4_1g15090 FvH4_1g15120 FvH4_1g15130 FvH4_1g15140 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15170 FvH4_1g15171 FvH4_1g15171 FvH4_1g15200 FvH4_1g15200 FvH4_1g15200 FvH4_1g15210 FvH4_1g15210 FvH4_1g15210 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15230 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15500 FvH4_1g15500 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_2g19760 FvH4_3g12820 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_6g18970 FvH4_6g42370 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g50100 FvH4_6g50100 FvH4_6g50110 FvH4_6g50130 FvH4_6g50140 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50330
malus_domestica MD00G1053700.v1.1 MD02G1164300.v1.1 MD02G1164400.v1.1 MD02G1164700.v1.1 MD02G1164800.v1.1 MD02G1165300.v1.1 MD02G1165400.v1.1 MD02G1165500.v1.1 MD02G1165600.v1.1 MD02G1165900.v1.1 MD02G1166000.v1.1 MD02G1166100.v1.1 MD02G1166300.v1.1 MD08G1084900.v1.1 MD15G1263100.v1.1 MD15G1263300.v1.1 MD15G1276900.v1.1 MD15G1277200.v1.1 MD15G1277400.v1.1 MD15G1277700.v1.1 MD15G1277800.v1.1 MD15G1278000.v1.1 MD15G1278100.v1.1 MD15G1278400.v1.1 MD15G1278600.v1.1
prunus_persica Prupe.7G138500_v2.0.a1 Prupe.7G138500_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138700_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138900_v2.0.a1 Prupe.7G139100_v2.0.a1 Prupe.7G139200_v2.0.a1 Prupe.7G139500_v2.0.a1 Prupe.7G139600_v2.0.a1 Prupe.7G139700_v2.0.a1 Prupe.7G139800_v2.0.a1
pyrus_communis pycom02g13150 pycom02g13160 pycom02g13190 pycom02g13200 pycom02g13220 pycom02g13230 pycom02g13250 pycom02g13260 pycom02g13270 pycom02g13300 pycom02g13310 pycom02g13330 pycom15g23980 pycom15g23990 pycom15g24030 pycom15g24040 pycom15g24050 pycom15g24070 pycom15g24090 pycom15g24160
rosa_chinensis RchiOBHm_Chr1g0326431 RchiOBHm_Chr1g0326451 RchiOBHm_Chr2g0104121 RchiOBHm_Chr2g0104151 RchiOBHm_Chr2g0104171 RchiOBHm_Chr2g0104181 RchiOBHm_Chr2g0104191 RchiOBHm_Chr2g0104201 RchiOBHm_Chr2g0104211 RchiOBHm_Chr2g0104241 RchiOBHm_Chr2g0104261 RchiOBHm_Chr2g0104281 RchiOBHm_Chr2g0104311 RchiOBHm_Chr2g0104331 RchiOBHm_Chr2g0104361 RchiOBHm_Chr2g0104381 RchiOBHm_Chr2g0104391 RchiOBHm_Chr2g0104431 RchiOBHm_Chr2g0104441 RchiOBHm_Chr2g0104541 RchiOBHm_Chr2g0104611 RchiOBHm_Chr2g0104741 RchiOBHm_Chr2g0104761 RchiOBHm_Chr2g0158291 RchiOBHm_Chr2g0170371 RchiOBHm_Chr2g0170631 RchiOBHm_Chr3g0473971 RchiOBHm_Chr3g0480491 RchiOBHm_Chr3g0480541 RchiOBHm_Chr3g0480561 RchiOBHm_Chr3g0485281 RchiOBHm_Chr3g0485291 RchiOBHm_Chr4g0411541 RchiOBHm_Chr6g0285471
rosa_laevigata RLG00000008347 RLG00000012637 RLG00000016961 RLG00000017293 RLG00000017305 RLG00000017308 RLG00000017312 RLG00000017314 RLG00000017317 RLG00000017318 RLG00000017319 RLG00000017321 RLG00000017322 RLG00000017323 RLG00000017324 RLG00000017325 RLG00000017327 RLG00000017328 RLG00000017329 RLG00000017330 RLG00000017334 RLG00000020833 RLG00000021073 RLG00000021950 RLG00000021971 RLG00000023154 RLG00000023157 RLG00000023467 RLG00000023468 RLG00000023525 RLG00000023963 RLG00000033265 RLG00000033267 RLG00000033268
rosa_multiflora Rmu_co8053082.1_g000001 Rmu_co8210358.1_g000001 Rmu_co8331661.1_g000001 Rmu_co8477427.1_g000002 Rmu_co8483217.1_g000001 Rmu_sc0000284.1_g000004 Rmu_sc0000676.1_g000017 Rmu_sc0000676.1_g000019 Rmu_sc0000676.1_g000021 Rmu_sc0002205.1_g000038 Rmu_sc0002205.1_g000041 Rmu_sc0002316.1_g000010 Rmu_sc0002316.1_g000012 Rmu_sc0002316.1_g000040 Rmu_sc0002316.1_g000071 Rmu_sc0002316.1_g000072 Rmu_sc0002316.1_g000095 Rmu_sc0002316.1_g000102 Rmu_sc0002414.1_g000018 Rmu_sc0002576.1_g000002 Rmu_sc0002576.1_g000003 Rmu_sc0002687.1_g000010 Rmu_sc0002687.1_g000017 Rmu_sc0003660.1_g000001 Rmu_sc0003787.1_g000014 Rmu_sc0003787.1_g000020 Rmu_sc0003787.1_g000024 Rmu_sc0003787.1_g000029 Rmu_sc0004059.1_g000026 Rmu_sc0005137.1_g000021 Rmu_sc0006550.1_g000001 Rmu_sc0006561.1_g000025 Rmu_sc0006561.1_g000033 Rmu_sc0007704.1_g000005 Rmu_sc0008904.1_g000005 Rmu_sc0010270.1_g000002 Rmu_sc0010307.1_g000001 Rmu_sc0011601.1_g000013 Rmu_sc0012694.1_g000005 Rmu_sc0013101.1_g000008 Rmu_sc0019029.1_g000001 Rmu_sc0019728.1_g000001 Rmu_sc0025829.1_g000001 Rmu_sc0025829.1_g000002 Rmu_ssc0000257.1_g000007 Rmu_ssc0000257.1_g000008
rosa_roxburghii Rroxscaffold_1G00021710 Rroxscaffold_1G00049350 Rroxscaffold_1G00049360 Rroxscaffold_2G00081160 Rroxscaffold_2G00081360 Rroxscaffold_2G00081390 Rroxscaffold_2G00091540 Rroxscaffold_2G00138790 Rroxscaffold_2G00138800 Rroxscaffold_2G00138920 Rroxscaffold_2G00139000 Rroxscaffold_2G00139010 Rroxscaffold_2G00139020 Rroxscaffold_2G00139030 Rroxscaffold_2G00139040 Rroxscaffold_2G00139050 Rroxscaffold_2G00139060 Rroxscaffold_2G00139070 Rroxscaffold_2G00139090 Rroxscaffold_2G00139100 Rroxscaffold_3G00257470 Rroxscaffold_4G00318040 Rroxscaffold_4G00323740 Rroxscaffold_4G00323750 Rroxscaffold_5G00356140 Rroxscaffold_6G00401280 Rroxscaffold_6G00401290 Rroxscaffold_7G00183120
rosa_rugosa Rorug01G0060500 Rorug01G0060900 Rorug02G0119500 Rorug02G0119600 Rorug02G0119700 Rorug02G0119700 Rorug02G0119700 Rorug02G0119800.1 Rorug02G0119900.1 Rorug02G0120000 Rorug02G0120100 Rorug02G0120200 Rorug02G0120300 Rorug02G0120400 Rorug02G0120500 Rorug02G0120700 Rorug02G0120700 Rorug02G0122300 Rorug02G0122300 Rorug02G0122400.1 Rorug02G0466700 Rorug02G0466700 Rorug02G0466800 Rorug02G0548600 Rorug02G0549900 Rorug02G0550000 Rorug03G0184200 Rorug04G0107900 Rorug05G0121500 Rorug06G0174800
rosa_samantha Rh1AG198400 Rh1CG074700 Rh1CG075000 Rh1CG183700 Rh2AG169700 Rh2AG169800 Rh2AG169900 Rh2AG170000 Rh2AG170300 Rh2AG170400 Rh2AG171000 Rh2AG171100 Rh2AG171600 Rh2AG172400 Rh2AG172500 Rh2AG532900 Rh2AG620000 Rh2AG622500 Rh2AG622800 Rh2AG623000 Rh2BG176200 Rh2BG176300 Rh2BG176400 Rh2BG176500 Rh2BG176600 Rh2BG176900 Rh2BG177000 Rh2BG177100 Rh2BG177200 Rh2BG177300 Rh2BG177600 Rh2BG177700 Rh2BG177900 Rh2BG178000 Rh2BG178200 Rh2BG178300 Rh2BG178500 Rh2BG179000 Rh2BG180000 Rh2BG180100 Rh2BG259200 Rh2BG546700 Rh2BG630800 Rh2BG633500 Rh2CG176500 Rh2CG177500 Rh2CG177700 Rh2DG174400 Rh2DG174600 Rh2DG174900 Rh2DG175100 Rh2DG175200 Rh2DG175300 Rh2DG175600 Rh2DG175700 Rh2DG175800 Rh2DG175900 Rh2DG176000 Rh2DG176300 Rh2DG176400 Rh2DG176500 Rh2DG176600 Rh2DG176900 Rh2DG177100 Rh2DG177200 Rh2DG177800 Rh2DG177900 Rh2DG178700 Rh2DG178800 Rh2DG555700 Rh2DG643700 Rh2DG646300 Rh3AG235600 Rh3AG235900 Rh3AG236200 Rh3AG266700 Rh3AG266900 Rh3BG216200 Rh3BG269100 Rh3CG212700 Rh3CG265800 Rh4AG168900 Rh4BG165500 Rh4CG180300 Rh5BG261500 Rh6AG286300 Rh6BG287500 Rh6CG288600 Rh7AG022600
rosa_wichuraiana Rw0G008240 Rw0G008250 Rw0G013190 Rw0G013200 Rw2G013230 Rw2G013240 Rw2G013270 Rw2G013280 Rw2G013290 Rw2G013300 Rw2G013330 Rw2G013340 Rw2G013350 Rw2G013360 Rw2G013370 Rw2G013380 Rw2G013410 Rw2G013570 Rw2G044120 Rw2G051370 Rw2G051590 Rw3G017160 Rw3G021150 Rw3G021160 Rw3G023730 Rw3G023740 Rw4G014030 Rw6G024590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 683
AarI CACCTGC 1 cut(s) 197
Acc36I ACCTGC 4 cut(s) 197, 760, 1644, 2050
AccB7I CCANNNNNTGG 1 cut(s) 1199
AccI GTMKAC 1 cut(s) 1527
AccII CGCG 1 cut(s) 102
AciI CCGC 3 cut(s) 194, 1812, 2523
AclI AACGTT 1 cut(s) 1895
AclWI GGATC 5 cut(s) 190, 375, 855, 868, 1857
AcoI YGGCCR 2 cut(s) 879, 1623
AcuI CTGAAG 7 cut(s) 497, 1082, 1473, 1566, 2027, 2177, 2523
AfiI CCNNNNNNNGG 2 cut(s) 1044, 1199
AflII CTTAAG 2 cut(s) 815, 2087
AflIII ACRYGT 2 cut(s) 100, 1581
AjnI CCWGG 4 cut(s) 853, 1192, 2067, 2445
AjuI GAANNNNNNNTTGG 8 cut(s) 551, 583, 647, 679, 860, 892, 1604, 1636
Alw21I GWGCWC 3 cut(s) 1722, 2435, 2555
Alw26I GTCTC 4 cut(s) 584, 653, 1809, 2618
AlwI GGATC 5 cut(s) 190, 375, 855, 868, 1857
AlwNI CAGNNNCTG 1 cut(s) 520
AoxI GGCC 5 cut(s) 30, 415, 879, 1549, 1623
ApaI GGGCCC 1 cut(s) 419
ApeKI GCWGC 7 cut(s) 203, 520, 601, 985, 1048, 1476, 1928
AseI ATTAAT 1 cut(s) 2001
Asp700I GAANNNNTTC 1 cut(s) 1458
AspA2I CCTAGG 1 cut(s) 1270
AspLEI GCGC 2 cut(s) 440, 705
AspS9I GGNCC 4 cut(s) 196, 415, 416, 620
AsuC2I CCSGG 1 cut(s) 865
AsuHPI GGTGA 9 cut(s) 142, 161, 274, 626, 731, 1183, 1720, 2054, 2525
AvaII GGWCC 2 cut(s) 196, 620
AvrII CCTAGG 1 cut(s) 1270
BaeGI GKGCMC 1 cut(s) 419
BaeI ACNNNNGTAYC 2 cut(s) 993, 1026
BalI TGGCCA 1 cut(s) 881
BamHI GGATCC 1 cut(s) 860
BanII GRGCYC 2 cut(s) 419, 2646
BbsI GAAGAC 1 cut(s) 2511
Bbv12I GWGCWC 3 cut(s) 1722, 2435, 2555
BbvI GCAGC 7 cut(s) 190, 507, 588, 972, 1035, 1488, 1940
BccI CCATC 5 cut(s) 605, 624, 688, 787, 1271
BceAI ACGGC 2 cut(s) 1022, 1610
BciT130I CCWGG 4 cut(s) 855, 1194, 2069, 2447
BclI TGATCA 4 cut(s) 166, 264, 709, 1797
BcnI CCSGG 1 cut(s) 865
BcoDI GTCTC 4 cut(s) 584, 653, 1809, 2618
BfaI CTAG 8 cut(s) 839, 1271, 1607, 1775, 1925, 1943, 2384, 2556
BfmI CTRYAG 3 cut(s) 204, 624, 1711
BfrI CTTAAG 2 cut(s) 815, 2087
BfuAI ACCTGC 4 cut(s) 197, 760, 1644, 2050
BisI GCNGC 7 cut(s) 204, 521, 602, 986, 1049, 1477, 1929
BlnI CCTAGG 1 cut(s) 1270
BlsI GCNGC 7 cut(s) 205, 522, 603, 987, 1050, 1478, 1930
BmcAI AGTACT 1 cut(s) 1003
Bme1390I CCNGG 5 cut(s) 855, 865, 1194, 2069, 2447
Bme18I GGWCC 2 cut(s) 196, 620
BmgT120I GGNCC 4 cut(s) 196, 415, 416, 620
BmiI GGNNCC 4 cut(s) 417, 621, 862, 2643
BmrFI CCNGG 5 cut(s) 855, 865, 1194, 2069, 2447
BmrI ACTGGG 1 cut(s) 2716
BmsI GCATC 8 cut(s) 146, 764, 997, 1228, 1274, 1474, 2193, 2215
BmuI ACTGGG 1 cut(s) 2716
BoxI GACNNNNGTC 1 cut(s) 695
BpiI GAAGAC 1 cut(s) 2511
BpmI CTGGAG 1 cut(s) 876
Bpu10I CCTNAGC 2 cut(s) 420, 2399
BpuEI CTTGAG 1 cut(s) 1765
BpuMI CCSGG 1 cut(s) 865
BsaI GGTCTC 1 cut(s) 653
BsaJI CCNNGG 8 cut(s) 567, 663, 761, 910, 1270, 1375, 1531, 2446
Bsc4I CCNNNNNNNGG 2 cut(s) 1044, 1199
Bse1I ACTGG 2 cut(s) 147, 2722
Bse3DI GCAATG 3 cut(s) 1248, 2228, 2272
BseBI CCWGG 4 cut(s) 855, 1194, 2069, 2447
BseDI CCNNGG 8 cut(s) 567, 663, 761, 910, 1270, 1375, 1531, 2446
BseGI GGATG 7 cut(s) 559, 635, 648, 787, 910, 1102, 1265
BseLI CCNNNNNNNGG 2 cut(s) 1044, 1199
BseMI GCAATG 3 cut(s) 1248, 2228, 2272
BseMII CTCAG 5 cut(s) 411, 1759, 1899, 2335, 2533
BseNI ACTGG 2 cut(s) 147, 2722
BseRI GAGGAG 3 cut(s) 281, 1873, 2618
BseSI GKGCMC 1 cut(s) 419
BseXI GCAGC 7 cut(s) 190, 507, 588, 972, 1035, 1488, 1940
BseYI CCCAGC 1 cut(s) 50
Bsh1236I CGCG 1 cut(s) 102
BshFI GGCC 5 cut(s) 32, 417, 881, 1551, 1625
BsiHKAI GWGCWC 3 cut(s) 1722, 2435, 2555
BsiSI CCGG 1 cut(s) 864
BslFI GGGAC 3 cut(s) 89, 703, 2704
BslI CCNNNNNNNGG 2 cut(s) 1044, 1199
BsmAI GTCTC 4 cut(s) 584, 653, 1809, 2618
BsmFI GGGAC 3 cut(s) 89, 703, 2704
BsnI GGCC 5 cut(s) 32, 417, 881, 1551, 1625
Bso31I GGTCTC 1 cut(s) 653
Bsp120I GGGCCC 1 cut(s) 415
Bsp1286I GDGCHC 5 cut(s) 419, 1722, 2435, 2555, 2646
Bsp1407I TGTACA 2 cut(s) 976, 2408
BspACI CCGC 3 cut(s) 194, 1812, 2523
BspANI GGCC 5 cut(s) 32, 417, 881, 1551, 1625
BspCNI CTCAG 5 cut(s) 412, 1758, 1898, 2334, 2532
BspFNI CGCG 1 cut(s) 102
BspLI GGNNCC 4 cut(s) 417, 621, 862, 2643
BspMAI CTGCAG 2 cut(s) 208, 628
BspMI ACCTGC 4 cut(s) 197, 760, 1644, 2050
BspPI GGATC 5 cut(s) 190, 375, 855, 868, 1857
BspQI GCTCTTC 1 cut(s) 2144
BspTI CTTAAG 2 cut(s) 815, 2087
BspTNI GGTCTC 1 cut(s) 653
BsrDI GCAATG 3 cut(s) 1248, 2228, 2272
BsrGI TGTACA 2 cut(s) 976, 2408
BsrI ACTGG 2 cut(s) 147, 2722
BssECI CCNNGG 8 cut(s) 567, 663, 761, 910, 1270, 1375, 1531, 2446
BssT1I CCWWGG 6 cut(s) 567, 663, 761, 910, 1270, 1531
Bst2UI CCWGG 4 cut(s) 855, 1194, 2069, 2447
Bst6I CTCTTC 3 cut(s) 516, 2144, 2616
BstAFI CTTAAG 2 cut(s) 815, 2087
BstAPI GCANNNNNTGC 1 cut(s) 212
BstAUI TGTACA 2 cut(s) 976, 2408
BstC8I GCNNGC 2 cut(s) 48, 1755
BstDEI CTNAG 9 cut(s) 420, 446, 1480, 1745, 1885, 2312, 2321, 2399, 2519
BstF5I GGATG 7 cut(s) 559, 635, 648, 787, 910, 1102, 1265
BstFNI CGCG 1 cut(s) 102
BstHHI GCGC 2 cut(s) 440, 705
BstMAI GTCTC 4 cut(s) 584, 653, 1809, 2618
BstMWI GCNNNNNNNGC 9 cut(s) 200, 212, 1281, 1473, 1603, 1750, 1870, 2380, 2650
BstNI CCWGG 4 cut(s) 855, 1194, 2069, 2447
BstNSI RCATGY 1 cut(s) 1585
BstPAI GACNNNNGTC 1 cut(s) 695
BstSCI CCNGG 5 cut(s) 853, 863, 1192, 2067, 2445
BstSFI CTRYAG 3 cut(s) 204, 624, 1711
BstSLI GKGCMC 1 cut(s) 419
BstUI CGCG 1 cut(s) 102
BstV1I GCAGC 7 cut(s) 190, 507, 588, 972, 1035, 1488, 1940
BstV2I GAAGAC 1 cut(s) 2511
BstX2I RGATCY 1 cut(s) 860
BstYI RGATCY 1 cut(s) 860
BsuRI GGCC 5 cut(s) 32, 417, 881, 1551, 1625
BtgZI GCGATG 3 cut(s) 290, 1290, 2631
BtsCI GGATG 7 cut(s) 559, 635, 648, 787, 910, 1102, 1265
BtsI GCAGTG 1 cut(s) 1327
BtsIMutI CAGTG 6 cut(s) 140, 329, 748, 1327, 1720, 2555
BveI ACCTGC 4 cut(s) 197, 760, 1644, 2050
Cac8I GCNNGC 2 cut(s) 48, 1755
CaiI CAGNNNCTG 1 cut(s) 520
CfoI GCGC 2 cut(s) 440, 705
Cfr13I GGNCC 4 cut(s) 196, 415, 416, 620
CseI GACGC 2 cut(s) 91, 1244
CspCI CAANNNNNGTGG 1 cut(s) 37
CviAII CATG 6 cut(s) 1582, 1598, 1736, 2030, 2176, 2542
DdeI CTNAG 9 cut(s) 420, 446, 1480, 1745, 1885, 2312, 2321, 2399, 2519
DraI TTTAAA 1 cut(s) 397
EaeI YGGCCR 2 cut(s) 879, 1623
Eam1104I CTCTTC 3 cut(s) 516, 2144, 2616
EarI CTCTTC 3 cut(s) 516, 2144, 2616
EciI GGCGGA 1 cut(s) 209
Eco130I CCWWGG 6 cut(s) 567, 663, 761, 910, 1270, 1531
Eco24I GRGCYC 2 cut(s) 419, 2646
Eco31I GGTCTC 1 cut(s) 653
Eco32I GATATC 2 cut(s) 1340, 2166
Eco47I GGWCC 2 cut(s) 196, 620
Eco57I CTGAAG 7 cut(s) 497, 1082, 1473, 1566, 2027, 2177, 2523
EcoO109I RGGNCCY 3 cut(s) 415, 416, 620
EcoRI GAATTC 2 cut(s) 2134, 2693
EcoRII CCWGG 4 cut(s) 853, 1192, 2067, 2445
EcoRV GATATC 2 cut(s) 1340, 2166
EcoT14I CCWWGG 6 cut(s) 567, 663, 761, 910, 1270, 1531
EcoT22I ATGCAT 1 cut(s) 2230
EcoT38I GRGCYC 2 cut(s) 419, 2646
ErhI CCWWGG 6 cut(s) 567, 663, 761, 910, 1270, 1531
FaeI CATG 6 cut(s) 1585, 1601, 1739, 2033, 2179, 2545
FalI AAGNNNNNCTT 6 cut(s) 551, 583, 947, 979, 1446, 1478
FaqI GGGAC 3 cut(s) 89, 703, 2704
FatI CATG 6 cut(s) 1581, 1597, 1735, 2029, 2175, 2541
FauNDI CATATG 1 cut(s) 2612
FbaI TGATCA 4 cut(s) 166, 264, 709, 1797
FblI GTMKAC 1 cut(s) 1527
Fnu4HI GCNGC 7 cut(s) 204, 521, 602, 986, 1049, 1477, 1929
FokI GGATG 7 cut(s) 566, 642, 655, 794, 917, 1109, 1252
FriOI GRGCYC 2 cut(s) 419, 2646
Fsp4HI GCNGC 7 cut(s) 204, 521, 602, 986, 1049, 1477, 1929
FspBI CTAG 8 cut(s) 839, 1271, 1607, 1775, 1925, 1943, 2384, 2556
GlaI GCGC 2 cut(s) 439, 704
GluI GCNGC 7 cut(s) 204, 521, 602, 986, 1049, 1477, 1929
GsaI CCCAGC 1 cut(s) 54
GsuI CTGGAG 1 cut(s) 876
HaeIII GGCC 5 cut(s) 32, 417, 881, 1551, 1625
HapII CCGG 1 cut(s) 864
HgaI GACGC 2 cut(s) 91, 1244
HhaI GCGC 2 cut(s) 440, 705
Hin1II CATG 6 cut(s) 1585, 1601, 1739, 2033, 2179, 2545
Hin6I GCGC 2 cut(s) 438, 703
HinP1I GCGC 2 cut(s) 438, 703
HindIII AAGCTT 4 cut(s) 117, 809, 2193, 2458
HpaII CCGG 1 cut(s) 864
HphI GGTGA 9 cut(s) 142, 161, 274, 626, 731, 1183, 1720, 2054, 2525
Hpy166II GTNNAC 1 cut(s) 1528
Hpy8I GTNNAC 1 cut(s) 1528
HpyCH4IV ACGT 1 cut(s) 1895
HpyF10VI GCNNNNNNNGC 9 cut(s) 200, 212, 1281, 1473, 1603, 1750, 1870, 2380, 2650
HpyF3I CTNAG 9 cut(s) 420, 446, 1480, 1745, 1885, 2312, 2321, 2399, 2519
HpySE526I ACGT 1 cut(s) 1895
Hsp92II CATG 6 cut(s) 1585, 1601, 1739, 2033, 2179, 2545
HspAI GCGC 2 cut(s) 438, 703
Ksp22I TGATCA 4 cut(s) 166, 264, 709, 1797
LguI GCTCTTC 1 cut(s) 2144
LmnI GCTCC 6 cut(s) 43, 85, 856, 1281, 2641, 2664
Lsp1109I GCAGC 7 cut(s) 190, 507, 588, 972, 1035, 1488, 1940
LweI GCATC 8 cut(s) 146, 764, 997, 1228, 1274, 1474, 2193, 2215
MaeI CTAG 8 cut(s) 839, 1271, 1607, 1775, 1925, 1943, 2384, 2556
MaeII ACGT 1 cut(s) 1895
MaeIII GTNAC 7 cut(s) 574, 766, 829, 1171, 1675, 1726, 1805
MfeI CAATTG 1 cut(s) 2203
MflI RGATCY 1 cut(s) 860
MhlI GDGCHC 5 cut(s) 419, 1722, 2435, 2555, 2646
MlsI TGGCCA 1 cut(s) 881
MluI ACGCGT 1 cut(s) 100
MluNI TGGCCA 1 cut(s) 881
MlyI GAGTC 4 cut(s) 60, 311, 1772, 2554
MmeI TCCRAC 3 cut(s) 60, 345, 1342
Mox20I TGGCCA 1 cut(s) 881
Mph1103I ATGCAT 1 cut(s) 2230
MroXI GAANNNNTTC 1 cut(s) 1458
MscI TGGCCA 1 cut(s) 881
MslI CAYNNNNRTG 1 cut(s) 1580
Msp20I TGGCCA 1 cut(s) 881
MspA1I CMGCKG 3 cut(s) 1753, 1931, 2523
MspCI CTTAAG 2 cut(s) 815, 2087
MspI CCGG 1 cut(s) 864
MspR9I CCNGG 5 cut(s) 855, 865, 1194, 2069, 2447
MunI CAATTG 1 cut(s) 2203
MvaI CCWGG 4 cut(s) 855, 1194, 2069, 2447
MvnI CGCG 1 cut(s) 102
MwoI GCNNNNNNNGC 9 cut(s) 200, 212, 1281, 1473, 1603, 1750, 1870, 2380, 2650
NciI CCSGG 1 cut(s) 865
NdeI CATATG 1 cut(s) 2612
NlaIII CATG 6 cut(s) 1585, 1601, 1739, 2033, 2179, 2545
NlaIV GGNNCC 4 cut(s) 417, 621, 862, 2643
NmuCI GTSAC 3 cut(s) 1171, 1675, 1726
NsiI ATGCAT 1 cut(s) 2230
NspI RCATGY 1 cut(s) 1585
PaqCI CACCTGC 1 cut(s) 197
PciI ACATGT 1 cut(s) 1581
PciSI GCTCTTC 1 cut(s) 2144
PdmI GAANNNNTTC 1 cut(s) 1458
PfeI GAWTC 7 cut(s) 129, 1112, 1430, 1442, 1557, 2072, 2094
PflMI CCANNNNNTGG 1 cut(s) 1199
PfoI TCCNGGA 4 cut(s) 853, 863, 1192, 2067
PkrI GCNGC 7 cut(s) 205, 522, 603, 987, 1050, 1478, 1930
PleI GAGTC 4 cut(s) 60, 311, 1772, 2553
PpsI GAGTC 4 cut(s) 60, 311, 1772, 2553
PpuMI RGGWCCY 1 cut(s) 620
PscI ACATGT 1 cut(s) 1581
PshAI GACNNNNGTC 1 cut(s) 695
PshBI ATTAAT 1 cut(s) 2001
PsiI TTATAA 1 cut(s) 683
Psp1406I AACGTT 1 cut(s) 1895
Psp5II RGGWCCY 1 cut(s) 620
Psp6I CCWGG 4 cut(s) 853, 1192, 2067, 2445
PspFI CCCAGC 1 cut(s) 50
PspGI CCWGG 4 cut(s) 853, 1192, 2067, 2445
PspN4I GGNNCC 4 cut(s) 417, 621, 862, 2643
PspOMI GGGCCC 1 cut(s) 415
PspPI GGNCC 4 cut(s) 196, 415, 416, 620
PspPPI RGGWCCY 1 cut(s) 620
PstI CTGCAG 2 cut(s) 208, 628
PstNI CAGNNNCTG 1 cut(s) 520
PsuI RGATCY 1 cut(s) 860
PvuII CAGCTG 2 cut(s) 1753, 1931
RseI CAYNNNNRTG 1 cut(s) 1580
SapI GCTCTTC 1 cut(s) 2144
SatI GCNGC 7 cut(s) 204, 521, 602, 986, 1049, 1477, 1929
Sau96I GGNCC 4 cut(s) 196, 415, 416, 620
SbfI CCTGCAGG 1 cut(s) 628
ScaI AGTACT 1 cut(s) 1003
SchI GAGTC 4 cut(s) 60, 311, 1772, 2554
ScrFI CCNGG 5 cut(s) 855, 865, 1194, 2069, 2447
SdaI CCTGCAGG 1 cut(s) 628
SduI GDGCHC 5 cut(s) 419, 1722, 2435, 2555, 2646
SfaNI GCATC 8 cut(s) 146, 764, 997, 1228, 1274, 1474, 2193, 2215
SfcI CTRYAG 3 cut(s) 204, 624, 1711
SinI GGWCC 2 cut(s) 196, 620
SmiMI CAYNNNNRTG 1 cut(s) 1580
SmlI CTYRAG 3 cut(s) 815, 1780, 2087
SmoI CTYRAG 3 cut(s) 815, 1780, 2087
Sse8387I CCTGCAGG 1 cut(s) 628
SsiI CCGC 3 cut(s) 194, 1812, 2523
SspI AATATT 1 cut(s) 2254
SspMI CTAG 8 cut(s) 839, 1271, 1607, 1775, 1925, 1943, 2384, 2556
StyD4I CCNGG 5 cut(s) 853, 863, 1192, 2067, 2445
StyI CCWWGG 6 cut(s) 567, 663, 761, 910, 1270, 1531
TaiI ACGT 1 cut(s) 1898
TaqI TCGA 4 cut(s) 336, 388, 959, 2138
TaqII GACCGA 1 cut(s) 2177
TatI WGTACW 9 cut(s) 235, 351, 400, 471, 477, 976, 1001, 1081, 2408
TfiI GAWTC 7 cut(s) 129, 1112, 1430, 1442, 1557, 2072, 2094
TscAI CASTG 6 cut(s) 147, 336, 748, 1334, 1720, 2555
TseFI GTSAC 3 cut(s) 1171, 1675, 1726
TseI GCWGC 7 cut(s) 203, 520, 601, 985, 1048, 1476, 1928
Tsp45I GTSAC 3 cut(s) 1171, 1675, 1726
TspGWI ACGGA 1 cut(s) 2418
TspRI CASTG 6 cut(s) 147, 336, 748, 1334, 1720, 2555
Van91I CCANNNNNTGG 1 cut(s) 1199
Vha464I CTTAAG 2 cut(s) 815, 2087
VpaK11BI GGWCC 2 cut(s) 196, 620
VspI ATTAAT 1 cut(s) 2001
XbaI TCTAGA 1 cut(s) 2555
XceI RCATGY 1 cut(s) 1585
XcmI CCANNNNNNNNNTGG 1 cut(s) 122
XmaJI CCTAGG 1 cut(s) 1270
XmiI GTMKAC 1 cut(s) 1527
XmnI GAANNNNTTC 1 cut(s) 1458
XspI CTAG 8 cut(s) 839, 1271, 1607, 1775, 1925, 1943, 2384, 2556
ZrmI AGTACT 1 cut(s) 1003
Zsp2I ATGCAT 1 cut(s) 2230
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.