FvH4_1g17470

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0000039
Physical Location & Seq
Reverse (-)
10129353 .. 10134046
4694 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g17470.t1

Sequence Viewer

Length: 2199 bp
ATGGGGAAGTATAACCAAAAATTGGATCGCACAAAGGAGTTGGAAGCTTTAGAAGGACTTATTAAGCGGGGCATAGAGCTCATAGGCAAATGCTCTAAGCTTCGTAAGTGGAACATCTACAAGAAGTACAAATACGCCAGCGAGTTTCTTGAATTCAAAGAATCTCTCCAAAAGGAGTTAGATGTACTCAAGGTGCAGGTAGCAAGAGATGTGAAGAAGACTGAAGTTGGCGTAGGAAATATACAGATTGTGGTTCAAAACATTCAGGAGGAGGTTTCAATGCTTGTGAAGCAGAATAGATCTTTGGAAATCGAAGCGTTAAGTGCAGCACTACCTGACCTGCCAGCGCTGATAGTCGGATTGGATGTTCCTTTGGAAGAACTGAAGCTGAAGCTTCTTAAGGACGAGGTATCCATGCTTATTGTGACTGCTCCTGGAGGATGTGGCAAGACCACTTTGGCAACAAAGTTTTGTCAAGATCAAGACGTCAAAGATAAATTCCAGAAGAACATTTTCTTCGTCACTTTTTCTAAAAAGCCCAACTTGGAGCTTATTATGAAAGGGTTATATGAACAAAAGGGTTATCCAGTACCTACTTTCCAGAATGAAGCAACTGATCCAGTGAAATGGCTGAAAAAAGTTGAGATGGAAAAAGAGGATGATCCTTTATTGTTGATCTTGGATGATGTTTGGTCCGGATCAGAATCTCTTCTTGGGAAGTTTGAGTTCAGAATGTCAAATTACAAGATTTTAGTGACATCGAGGTCTGAATTTCCGAGATTTGGTCCTTCATATCATCTACAACTATTGGATGATGACAATGCTATGGAACTTTTTCGTCAGACAGCATCCTTGGGAGACAAGAGCTCTCATATTCCAAAAGATCTTTCAAAAGAGATAGTAAAACATTGTAATGGATTTCCACTGGCCATTACAGTGGCCGGAAGATCACTTTGCGGGCAACCTGAAGAAATGTGGCGAAGTAGAGTAGATGAATGGTCAAAAGGTTCTTCCATTCTTGATTTTGAGATGGAATTGCTGCTTCGGCTCCAAAGCAGTGTAGATGTCTTGGATGATGCAATGCCTAATGTCAAAGAATGTTTCTTAGACCTAGGTGCTTTTCCTGAAGATGCTGAAATCAAAGTTACAGCTCTCATTGATATGTGGTCAGAGTTGGATAAGGGTTTGGAAGTGGTACATGAGGCAATTGATTACAGCAAATACGTTGTTAGGCAGCATGATTTGCTAAGAGAACTGGCTATCTACAACTCTAAACGGGGTCCCATGCAGCATAGAGAAAGACTGGTTATAGAGATATGCGGAAACAATTTTACCAAATGGTGGAGACAACAAAAATATCCATACATGAATGCTCGCCTTGTATCAATCTCAACTGATGCAGAGTTGTCAGTGAAATTGCCAAACTTGCAATTACCAGAGGCAGAGGTTCTAGTCTTGAATTTTCAATCAAAAACGTCCTATGCCTTACCCGAATTTGTGGAGCGAATGGACAAACTCAAGCTGCTGCTTGTCATAAATTATTCTAGCCTTTGTGAAGAATTGGATAATTTTCACCTACTGGGATCCTTATCAAATCTTAGGAGTGTCAGATTAGACGGCATAAATTGTTCTTGCATAACCAAGAGTCCAGTACAATTGAAGACTGTATACAAATTATATTTGAGAGAAGGTAGATCTTTCGGCGAAAATCCTTTGGGATCATCACCTGTATTTCCATATCTGAAGGAAATGGTCATTGGTGCCTGCAGGGCTATCTTGCGAAACGGTCCTGTCAAACTATCACATGTATTTCCAAATCTGAAGGAATTGGTCATTGATGCCTGCGATATGCGGAAATTGCCTGCAGAACAGCTGATTCATCTAACAAAACTCAAAATCACTAATTGTGGCTTGTTAACAGACTTGCCTGAAGAGATTGGAAATATGGTCAGTTTAGAAGTGTTGAGGATTGAGCACTGTCCACTGCTTGCAAAGTTTCCTAACTCAATTGGGAATCTTAAGAAACTAAAATTTCTTAAGATTTCTGGGACTATTCTGTTCCATAAGTTCCCTGATTCGATTGCAAATTTGGTCAATTTAGAAGTACTGATTCTAAGGAGCTGTCGCCGCTTGTTAGAGTTGCCAGAGTCGATTAGACATTTCCGAAAGTTAAACTTCCTTGACATCCATAGGTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

733

Amino Acids

83.67

Weight (kDa)

7.77

Isoelectric Point (pI)

44.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 2 - 76 1.3e-10 Arabidopsis broad-spectrum mildew resistance protein RPW8
NB-ARC PF00931 124 - 283 1.7e-13 NB-ARC domain
LRR_14 PF23598 623 - 729 5e-12 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000039)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66890 AT5G66900 AT5G66900 AT5G66900 AT5G66910
fragaria_vesca FvH4_1g15090 FvH4_1g15120 FvH4_1g15130 FvH4_1g15140 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15170 FvH4_1g15171 FvH4_1g15171 FvH4_1g15200 FvH4_1g15200 FvH4_1g15200 FvH4_1g15210 FvH4_1g15210 FvH4_1g15210 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15230 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15500 FvH4_1g15500 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_2g19760 FvH4_3g12820 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_6g18970 FvH4_6g42370 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g50100 FvH4_6g50100 FvH4_6g50110 FvH4_6g50130 FvH4_6g50140 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50330
malus_domestica MD00G1053700.v1.1 MD02G1164300.v1.1 MD02G1164400.v1.1 MD02G1164700.v1.1 MD02G1164800.v1.1 MD02G1165300.v1.1 MD02G1165400.v1.1 MD02G1165500.v1.1 MD02G1165600.v1.1 MD02G1165900.v1.1 MD02G1166000.v1.1 MD02G1166100.v1.1 MD02G1166300.v1.1 MD08G1084900.v1.1 MD15G1263100.v1.1 MD15G1263300.v1.1 MD15G1276900.v1.1 MD15G1277200.v1.1 MD15G1277400.v1.1 MD15G1277700.v1.1 MD15G1277800.v1.1 MD15G1278000.v1.1 MD15G1278100.v1.1 MD15G1278400.v1.1 MD15G1278600.v1.1
prunus_persica Prupe.7G138500_v2.0.a1 Prupe.7G138500_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138700_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138900_v2.0.a1 Prupe.7G139100_v2.0.a1 Prupe.7G139200_v2.0.a1 Prupe.7G139500_v2.0.a1 Prupe.7G139600_v2.0.a1 Prupe.7G139700_v2.0.a1 Prupe.7G139800_v2.0.a1
pyrus_communis pycom02g13150 pycom02g13160 pycom02g13190 pycom02g13200 pycom02g13220 pycom02g13230 pycom02g13250 pycom02g13260 pycom02g13270 pycom02g13300 pycom02g13310 pycom02g13330 pycom15g23980 pycom15g23990 pycom15g24030 pycom15g24040 pycom15g24050 pycom15g24070 pycom15g24090 pycom15g24160
rosa_chinensis RchiOBHm_Chr1g0326431 RchiOBHm_Chr1g0326451 RchiOBHm_Chr2g0104121 RchiOBHm_Chr2g0104151 RchiOBHm_Chr2g0104171 RchiOBHm_Chr2g0104181 RchiOBHm_Chr2g0104191 RchiOBHm_Chr2g0104201 RchiOBHm_Chr2g0104211 RchiOBHm_Chr2g0104241 RchiOBHm_Chr2g0104261 RchiOBHm_Chr2g0104281 RchiOBHm_Chr2g0104311 RchiOBHm_Chr2g0104331 RchiOBHm_Chr2g0104361 RchiOBHm_Chr2g0104381 RchiOBHm_Chr2g0104391 RchiOBHm_Chr2g0104431 RchiOBHm_Chr2g0104441 RchiOBHm_Chr2g0104541 RchiOBHm_Chr2g0104611 RchiOBHm_Chr2g0104741 RchiOBHm_Chr2g0104761 RchiOBHm_Chr2g0158291 RchiOBHm_Chr2g0170371 RchiOBHm_Chr2g0170631 RchiOBHm_Chr3g0473971 RchiOBHm_Chr3g0480491 RchiOBHm_Chr3g0480541 RchiOBHm_Chr3g0480561 RchiOBHm_Chr3g0485281 RchiOBHm_Chr3g0485291 RchiOBHm_Chr4g0411541 RchiOBHm_Chr6g0285471
rosa_laevigata RLG00000008347 RLG00000012637 RLG00000016961 RLG00000017293 RLG00000017305 RLG00000017308 RLG00000017312 RLG00000017314 RLG00000017317 RLG00000017318 RLG00000017319 RLG00000017321 RLG00000017322 RLG00000017323 RLG00000017324 RLG00000017325 RLG00000017327 RLG00000017328 RLG00000017329 RLG00000017330 RLG00000017334 RLG00000020833 RLG00000021073 RLG00000021950 RLG00000021971 RLG00000023154 RLG00000023157 RLG00000023467 RLG00000023468 RLG00000023525 RLG00000023963 RLG00000033265 RLG00000033267 RLG00000033268
rosa_multiflora Rmu_co8053082.1_g000001 Rmu_co8210358.1_g000001 Rmu_co8331661.1_g000001 Rmu_co8477427.1_g000002 Rmu_co8483217.1_g000001 Rmu_sc0000284.1_g000004 Rmu_sc0000676.1_g000017 Rmu_sc0000676.1_g000019 Rmu_sc0000676.1_g000021 Rmu_sc0002205.1_g000038 Rmu_sc0002205.1_g000041 Rmu_sc0002316.1_g000010 Rmu_sc0002316.1_g000012 Rmu_sc0002316.1_g000040 Rmu_sc0002316.1_g000071 Rmu_sc0002316.1_g000072 Rmu_sc0002316.1_g000095 Rmu_sc0002316.1_g000102 Rmu_sc0002414.1_g000018 Rmu_sc0002576.1_g000002 Rmu_sc0002576.1_g000003 Rmu_sc0002687.1_g000010 Rmu_sc0002687.1_g000017 Rmu_sc0003660.1_g000001 Rmu_sc0003787.1_g000014 Rmu_sc0003787.1_g000020 Rmu_sc0003787.1_g000024 Rmu_sc0003787.1_g000029 Rmu_sc0004059.1_g000026 Rmu_sc0005137.1_g000021 Rmu_sc0006550.1_g000001 Rmu_sc0006561.1_g000025 Rmu_sc0006561.1_g000033 Rmu_sc0007704.1_g000005 Rmu_sc0008904.1_g000005 Rmu_sc0010270.1_g000002 Rmu_sc0010307.1_g000001 Rmu_sc0011601.1_g000013 Rmu_sc0012694.1_g000005 Rmu_sc0013101.1_g000008 Rmu_sc0019029.1_g000001 Rmu_sc0019728.1_g000001 Rmu_sc0025829.1_g000001 Rmu_sc0025829.1_g000002 Rmu_ssc0000257.1_g000007 Rmu_ssc0000257.1_g000008
rosa_roxburghii Rroxscaffold_1G00021710 Rroxscaffold_1G00049350 Rroxscaffold_1G00049360 Rroxscaffold_2G00081160 Rroxscaffold_2G00081360 Rroxscaffold_2G00081390 Rroxscaffold_2G00091540 Rroxscaffold_2G00138790 Rroxscaffold_2G00138800 Rroxscaffold_2G00138920 Rroxscaffold_2G00139000 Rroxscaffold_2G00139010 Rroxscaffold_2G00139020 Rroxscaffold_2G00139030 Rroxscaffold_2G00139040 Rroxscaffold_2G00139050 Rroxscaffold_2G00139060 Rroxscaffold_2G00139070 Rroxscaffold_2G00139090 Rroxscaffold_2G00139100 Rroxscaffold_3G00257470 Rroxscaffold_4G00318040 Rroxscaffold_4G00323740 Rroxscaffold_4G00323750 Rroxscaffold_5G00356140 Rroxscaffold_6G00401280 Rroxscaffold_6G00401290 Rroxscaffold_7G00183120
rosa_rugosa Rorug01G0060500 Rorug01G0060900 Rorug02G0119500 Rorug02G0119600 Rorug02G0119700 Rorug02G0119700 Rorug02G0119700 Rorug02G0119800.1 Rorug02G0119900.1 Rorug02G0120000 Rorug02G0120100 Rorug02G0120200 Rorug02G0120300 Rorug02G0120400 Rorug02G0120500 Rorug02G0120700 Rorug02G0120700 Rorug02G0122300 Rorug02G0122300 Rorug02G0122400.1 Rorug02G0466700 Rorug02G0466700 Rorug02G0466800 Rorug02G0548600 Rorug02G0549900 Rorug02G0550000 Rorug03G0184200 Rorug04G0107900 Rorug05G0121500 Rorug06G0174800
rosa_samantha Rh1AG198400 Rh1CG074700 Rh1CG075000 Rh1CG183700 Rh2AG169700 Rh2AG169800 Rh2AG169900 Rh2AG170000 Rh2AG170300 Rh2AG170400 Rh2AG171000 Rh2AG171100 Rh2AG171600 Rh2AG172400 Rh2AG172500 Rh2AG532900 Rh2AG620000 Rh2AG622500 Rh2AG622800 Rh2AG623000 Rh2BG176200 Rh2BG176300 Rh2BG176400 Rh2BG176500 Rh2BG176600 Rh2BG176900 Rh2BG177000 Rh2BG177100 Rh2BG177200 Rh2BG177300 Rh2BG177600 Rh2BG177700 Rh2BG177900 Rh2BG178000 Rh2BG178200 Rh2BG178300 Rh2BG178500 Rh2BG179000 Rh2BG180000 Rh2BG180100 Rh2BG259200 Rh2BG546700 Rh2BG630800 Rh2BG633500 Rh2CG176500 Rh2CG177500 Rh2CG177700 Rh2DG174400 Rh2DG174600 Rh2DG174900 Rh2DG175100 Rh2DG175200 Rh2DG175300 Rh2DG175600 Rh2DG175700 Rh2DG175800 Rh2DG175900 Rh2DG176000 Rh2DG176300 Rh2DG176400 Rh2DG176500 Rh2DG176600 Rh2DG176900 Rh2DG177100 Rh2DG177200 Rh2DG177800 Rh2DG177900 Rh2DG178700 Rh2DG178800 Rh2DG555700 Rh2DG643700 Rh2DG646300 Rh3AG235600 Rh3AG235900 Rh3AG236200 Rh3AG266700 Rh3AG266900 Rh3BG216200 Rh3BG269100 Rh3CG212700 Rh3CG265800 Rh4AG168900 Rh4BG165500 Rh4CG180300 Rh5BG261500 Rh6AG286300 Rh6BG287500 Rh6CG288600 Rh7AG022600
rosa_wichuraiana Rw0G008240 Rw0G008250 Rw0G013190 Rw0G013200 Rw2G013230 Rw2G013240 Rw2G013270 Rw2G013280 Rw2G013290 Rw2G013300 Rw2G013330 Rw2G013340 Rw2G013350 Rw2G013360 Rw2G013370 Rw2G013380 Rw2G013410 Rw2G013570 Rw2G044120 Rw2G051370 Rw2G051590 Rw3G017160 Rw3G021150 Rw3G021160 Rw3G023730 Rw3G023740 Rw4G014030 Rw6G024590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 763
AatII GACGTC 1 cut(s) 489
Acc36I ACCTGC 2 cut(s) 187, 348
AccB1I GGYRCC 1 cut(s) 1760
AccB7I CCANNNNNTGG 2 cut(s) 22, 1341
AccI GTMKAC 1 cut(s) 1668
AccIII TCCGGA 1 cut(s) 695
AciI CCGC 5 cut(s) 67, 957, 1320, 1852, 2128
AclWI GGATC 7 cut(s) 33, 611, 656, 706, 1578, 1591, 1726
AcoI YGGCCR 2 cut(s) 927, 939
AcsI RAATTY 7 cut(s) 152, 497, 770, 1459, 1493, 2030, 2086
AcuI CTGAAG 8 cut(s) 243, 404, 410, 987, 1146, 1763, 1841, 1950
AcyI GRCGYC 1 cut(s) 486
AfaI GTAC 6 cut(s) 128, 186, 591, 1197, 1653, 2106
AfeI AGCGCT 1 cut(s) 348
AfiI CCNNNNNNNGG 3 cut(s) 22, 782, 1341
AflII CTTAAG 3 cut(s) 398, 2018, 2036
AflIII ACRYGT 1 cut(s) 1804
AgsI TTSAA 8 cut(s) 152, 157, 257, 279, 891, 1459, 1466, 1660
AjnI CCWGG 1 cut(s) 433
AjuI GAANNNNNNNTTGG 8 cut(s) 287, 319, 696, 728, 871, 903, 1740, 1772
AloI GAACNNNNNNTCC 2 cut(s) 351, 383
Alw21I GWGCWC 3 cut(s) 81, 869, 1977
Alw26I GTCTC 2 cut(s) 852, 1339
AlwI GGATC 7 cut(s) 33, 611, 656, 706, 1578, 1591, 1726
Aor13HI TCCGGA 1 cut(s) 695
Aor51HI AGCGCT 1 cut(s) 348
AoxI GGCC 2 cut(s) 927, 939
ApeKI GCWGC 6 cut(s) 326, 1039, 1234, 1288, 1522, 1525
ApoI RAATTY 7 cut(s) 152, 497, 770, 1459, 1493, 2030, 2086
Asp700I GAANNNNTTC 3 cut(s) 512, 708, 834
AspA2I CCTAGG 1 cut(s) 1111
AspLEI GCGC 1 cut(s) 349
AspS9I GGNCC 4 cut(s) 693, 785, 1280, 1787
AsuHPI GGTGA 2 cut(s) 1565, 1716
AvaII GGWCC 4 cut(s) 693, 785, 1280, 1787
AvrII CCTAGG 1 cut(s) 1111
BalI TGGCCA 1 cut(s) 929
BamHI GGATCC 1 cut(s) 1583
BanI GGYRCC 1 cut(s) 1760
BanII GRGCYC 2 cut(s) 81, 869
BarI GAAGNNNNNNTAC 4 cut(s) 116, 148, 1652, 1684
BbsI GAAGAC 2 cut(s) 224, 1667
Bbv12I GWGCWC 3 cut(s) 81, 869, 1977
BbvI GCAGC 6 cut(s) 338, 1026, 1246, 1300, 1509, 1512
BccI CCATC 2 cut(s) 640, 1024
BceAI ACGGC 1 cut(s) 1633
BciT130I CCWGG 1 cut(s) 435
BciVI GTATCC 1 cut(s) 421
BcoDI GTCTC 2 cut(s) 852, 1339
BfaI CTAG 3 cut(s) 1112, 1451, 1545
BfmI CTRYAG 2 cut(s) 1765, 1863
BfoI RGCGCY 1 cut(s) 350
BfrI CTTAAG 3 cut(s) 398, 2018, 2036
BfuAI ACCTGC 2 cut(s) 187, 348
BfuI GTATCC 1 cut(s) 421
BglI GCCNNNNNGGC 1 cut(s) 1769
BglII AGATCT 3 cut(s) 299, 883, 1694
BisI GCNGC 7 cut(s) 327, 1040, 1235, 1289, 1523, 1526, 2128
BlnI CCTAGG 1 cut(s) 1111
BlsI GCNGC 7 cut(s) 328, 1041, 1236, 1290, 1524, 1527, 2129
BmcAI AGTACT 1 cut(s) 2106
Bme1390I CCNGG 1 cut(s) 435
Bme18I GGWCC 4 cut(s) 693, 785, 1280, 1787
BmgT120I GGNCC 4 cut(s) 693, 785, 1280, 1787
BmiI GGNNCC 5 cut(s) 1049, 1281, 1282, 1585, 1762
BmrFI CCNGG 1 cut(s) 435
BmrI ACTGGG 1 cut(s) 1589
BmsI GCATC 5 cut(s) 857, 1066, 1120, 1387, 1828
BmuI ACTGGG 1 cut(s) 1589
BpiI GAAGAC 2 cut(s) 224, 1667
BpmI CTGGAG 1 cut(s) 456
BpuEI CTTGAG 2 cut(s) 173, 1502
BsaBI GATNNNNATC 2 cut(s) 703, 1588
BsaHI GRCGYC 1 cut(s) 486
BsaJI CCNNGG 2 cut(s) 852, 1111
BsaWI WCCGGW 1 cut(s) 695
Bsc4I CCNNNNNNNGG 3 cut(s) 22, 782, 1341
Bse1I ACTGG 7 cut(s) 587, 620, 930, 1260, 1308, 1584, 1649
Bse3DI GCAATG 1 cut(s) 1086
Bse8I GATNNNNATC 2 cut(s) 703, 1588
BseAI TCCGGA 1 cut(s) 695
BseBI CCWGG 1 cut(s) 435
BseDI CCNNGG 2 cut(s) 852, 1111
BseGI GGATG 8 cut(s) 370, 446, 664, 688, 817, 848, 1078, 2184
BseJI GATNNNNATC 2 cut(s) 703, 1588
BseLI CCNNNNNNNGG 3 cut(s) 22, 782, 1341
BseMI GCAATG 1 cut(s) 1086
BseNI ACTGG 7 cut(s) 587, 620, 930, 1260, 1308, 1584, 1649
BseRI GAGGAG 1 cut(s) 284
BseXI GCAGC 6 cut(s) 338, 1026, 1246, 1300, 1509, 1512
BsgI GTGCAG 2 cut(s) 215, 345
BshFI GGCC 2 cut(s) 929, 941
BshNI GGYRCC 1 cut(s) 1760
BsiHKAI GWGCWC 3 cut(s) 81, 869, 1977
BsiSI CCGG 2 cut(s) 696, 942
BslFI GGGAC 2 cut(s) 1266, 2062
BslI CCNNNNNNNGG 3 cut(s) 22, 782, 1341
BsmAI GTCTC 2 cut(s) 852, 1339
BsmFI GGGAC 2 cut(s) 1266, 2062
BsmI GAATGC 1 cut(s) 1375
BsnI GGCC 2 cut(s) 929, 941
Bsp1286I GDGCHC 3 cut(s) 81, 869, 1977
Bsp13I TCCGGA 1 cut(s) 695
BspACI CCGC 5 cut(s) 67, 957, 1320, 1852, 2128
BspANI GGCC 2 cut(s) 929, 941
BspEI TCCGGA 1 cut(s) 695
BspLI GGNNCC 5 cut(s) 1049, 1281, 1282, 1585, 1762
BspMAI CTGCAG 2 cut(s) 1769, 1867
BspMI ACCTGC 2 cut(s) 187, 348
BspPI GGATC 7 cut(s) 33, 611, 656, 706, 1578, 1591, 1726
BspT107I GGYRCC 1 cut(s) 1760
BspTI CTTAAG 3 cut(s) 398, 2018, 2036
BsrDI GCAATG 1 cut(s) 1086
BsrI ACTGG 7 cut(s) 587, 620, 930, 1260, 1308, 1584, 1649
BssECI CCNNGG 2 cut(s) 852, 1111
BssNAI GTATAC 1 cut(s) 1669
BssNI GRCGYC 1 cut(s) 486
BssT1I CCWWGG 2 cut(s) 852, 1111
Bst1107I GTATAC 1 cut(s) 1669
Bst2UI CCWGG 1 cut(s) 435
Bst4CI ACNGT 4 cut(s) 937, 1666, 1787, 1979
Bst6I CTCTTC 2 cut(s) 714, 1926
BstACI GRCGYC 1 cut(s) 486
BstAFI CTTAAG 3 cut(s) 398, 2018, 2036
BstAPI GCANNNNNTGC 1 cut(s) 1243
BstC8I GCNNGC 8 cut(s) 139, 345, 959, 1375, 1765, 1843, 1863, 1989
BstDEI CTNAG 5 cut(s) 96, 1105, 1247, 1598, 2114
BstF5I GGATG 8 cut(s) 370, 446, 664, 688, 817, 848, 1078, 2184
BstH2I RGCGCY 1 cut(s) 350
BstHHI GCGC 1 cut(s) 349
BstMAI GTCTC 2 cut(s) 852, 1339
BstMWI GCNNNNNNNGC 8 cut(s) 289, 323, 1045, 1243, 1426, 1769, 1858, 2127
BstNI CCWGG 1 cut(s) 435
BstNSI RCATGY 1 cut(s) 1808
BstSCI CCNGG 1 cut(s) 433
BstSFI CTRYAG 2 cut(s) 1765, 1863
BstV1I GCAGC 6 cut(s) 338, 1026, 1246, 1300, 1509, 1512
BstV2I GAAGAC 2 cut(s) 224, 1667
BstX2I RGATCY 4 cut(s) 299, 883, 1583, 1694
BstXI CCANNNNNNTGG 2 cut(s) 627, 937
BstYI RGATCY 4 cut(s) 299, 883, 1583, 1694
BstZ17I GTATAC 1 cut(s) 1669
BsuI GTATCC 1 cut(s) 421
BsuRI GGCC 2 cut(s) 929, 941
BtsCI GGATG 8 cut(s) 370, 446, 664, 688, 817, 848, 1078, 2184
BtsI GCAGTG 2 cut(s) 1063, 1982
BtsIMutI CAGTG 7 cut(s) 627, 923, 942, 1063, 1416, 1975, 1982
BveI ACCTGC 2 cut(s) 187, 348
Cac8I GCNNGC 8 cut(s) 139, 345, 959, 1375, 1765, 1843, 1863, 1989
CfoI GCGC 1 cut(s) 349
Cfr13I GGNCC 4 cut(s) 693, 785, 1280, 1787
Csp6I GTAC 6 cut(s) 127, 185, 590, 1196, 1652, 2105
CviAII CATG 6 cut(s) 415, 1199, 1238, 1285, 1366, 1805
CviQI GTAC 6 cut(s) 127, 185, 590, 1196, 1652, 2105
DdeI CTNAG 5 cut(s) 96, 1105, 1247, 1598, 2114
DrdI GACNNNNNNGTC 1 cut(s) 763
DseDI GACNNNNNNGTC 1 cut(s) 763
EaeI YGGCCR 2 cut(s) 927, 939
Eam1104I CTCTTC 2 cut(s) 714, 1926
EarI CTCTTC 2 cut(s) 714, 1926
Ecl136II GAGCTC 2 cut(s) 79, 867
Eco130I CCWWGG 2 cut(s) 852, 1111
Eco24I GRGCYC 2 cut(s) 81, 869
Eco47I GGWCC 4 cut(s) 693, 785, 1280, 1787
Eco47III AGCGCT 1 cut(s) 348
Eco53kI GAGCTC 2 cut(s) 79, 867
Eco57I CTGAAG 8 cut(s) 243, 404, 410, 987, 1146, 1763, 1841, 1950
EcoICRI GAGCTC 2 cut(s) 79, 867
EcoO109I RGGNCCY 1 cut(s) 1280
EcoRI GAATTC 1 cut(s) 152
EcoRII CCWGG 1 cut(s) 433
EcoT14I CCWWGG 2 cut(s) 852, 1111
EcoT38I GRGCYC 2 cut(s) 81, 869
ErhI CCWWGG 2 cut(s) 852, 1111
FaeI CATG 6 cut(s) 418, 1202, 1241, 1288, 1369, 1808
FalI AAGNNNNNCTT 4 cut(s) 527, 559, 2159, 2191
FaqI GGGAC 2 cut(s) 1266, 2062
FatI CATG 6 cut(s) 414, 1198, 1237, 1284, 1365, 1804
FauI CCCGC 2 cut(s) 60, 950
FblI GTMKAC 1 cut(s) 1668
Fnu4HI GCNGC 7 cut(s) 327, 1040, 1235, 1289, 1523, 1526, 2128
FokI GGATG 8 cut(s) 377, 453, 671, 695, 824, 835, 1085, 2171
FriOI GRGCYC 2 cut(s) 81, 869
Fsp4HI GCNGC 7 cut(s) 327, 1040, 1235, 1289, 1523, 1526, 2128
FspBI CTAG 3 cut(s) 1112, 1451, 1545
GlaI GCGC 1 cut(s) 348
GluI GCNGC 7 cut(s) 327, 1040, 1235, 1289, 1523, 1526, 2128
GsuI CTGGAG 1 cut(s) 456
HaeII RGCGCY 1 cut(s) 350
HaeIII GGCC 2 cut(s) 929, 941
HapII CCGG 2 cut(s) 696, 942
HhaI GCGC 1 cut(s) 349
Hin1I GRCGYC 1 cut(s) 486
Hin1II CATG 6 cut(s) 418, 1202, 1241, 1288, 1369, 1808
Hin6I GCGC 1 cut(s) 347
HinP1I GCGC 1 cut(s) 347
HincII GTYRAC 1 cut(s) 1917
HindII GTYRAC 1 cut(s) 1917
HindIII AAGCTT 3 cut(s) 45, 98, 392
HinfI GANTC 8 cut(s) 161, 704, 1645, 1876, 2014, 2075, 2110, 2147
HpaI GTTAAC 1 cut(s) 1917
HpaII CCGG 2 cut(s) 696, 942
HphI GGTGA 2 cut(s) 1565, 1716
Hpy166II GTNNAC 3 cut(s) 1669, 1917, 1982
Hpy8I GTNNAC 3 cut(s) 1669, 1917, 1982
HpyAV CCTTC 5 cut(s) 47, 798, 1682, 1738, 1816
HpyCH4III ACNGT 4 cut(s) 937, 1666, 1787, 1979
HpyCH4IV ACGT 3 cut(s) 486, 1224, 1475
HpyF10VI GCNNNNNNNGC 8 cut(s) 289, 323, 1045, 1243, 1426, 1769, 1858, 2127
HpyF3I CTNAG 5 cut(s) 96, 1105, 1247, 1598, 2114
HpySE526I ACGT 3 cut(s) 486, 1224, 1475
Hsp92I GRCGYC 1 cut(s) 486
Hsp92II CATG 6 cut(s) 418, 1202, 1241, 1288, 1369, 1808
HspAI GCGC 1 cut(s) 347
KflI GGGWCCC 1 cut(s) 1280
Kpn2I TCCGGA 1 cut(s) 695
KspAI GTTAAC 1 cut(s) 1917
LmnI GCTCC 5 cut(s) 436, 547, 1053, 1501, 2118
Lsp1109I GCAGC 6 cut(s) 338, 1026, 1246, 1300, 1509, 1512
LweI GCATC 5 cut(s) 857, 1066, 1120, 1387, 1828
MaeI CTAG 3 cut(s) 1112, 1451, 1545
MaeII ACGT 3 cut(s) 486, 1224, 1475
MaeIII GTNAC 4 cut(s) 424, 520, 754, 1144
MfeI CAATTG 3 cut(s) 1206, 1655, 2007
MflI RGATCY 4 cut(s) 299, 883, 1583, 1694
MhlI GDGCHC 3 cut(s) 81, 869, 1977
MlsI TGGCCA 1 cut(s) 929
MluNI TGGCCA 1 cut(s) 929
MlyI GAGTC 2 cut(s) 1654, 2156
MmeI TCCRAC 3 cut(s) 21, 337, 1155
Mox20I TGGCCA 1 cut(s) 929
MroI TCCGGA 1 cut(s) 695
MroXI GAANNNNTTC 3 cut(s) 512, 708, 834
MscI TGGCCA 1 cut(s) 929
MseI TTAA 7 cut(s) 63, 320, 399, 1916, 2019, 2037, 2171
MslI CAYNNNNRTG 3 cut(s) 912, 935, 1160
Msp20I TGGCCA 1 cut(s) 929
MspA1I CMGCKG 1 cut(s) 1873
MspCI CTTAAG 3 cut(s) 398, 2018, 2036
MspI CCGG 2 cut(s) 696, 942
MspR9I CCNGG 1 cut(s) 435
MunI CAATTG 3 cut(s) 1206, 1655, 2007
Mva1269I GAATGC 1 cut(s) 1375
MvaI CCWGG 1 cut(s) 435
MwoI GCNNNNNNNGC 8 cut(s) 289, 323, 1045, 1243, 1426, 1769, 1858, 2127
NlaIII CATG 6 cut(s) 418, 1202, 1241, 1288, 1369, 1808
NlaIV GGNNCC 5 cut(s) 1049, 1281, 1282, 1585, 1762
NmuCI GTSAC 3 cut(s) 424, 520, 754
NspI RCATGY 1 cut(s) 1808
PciI ACATGT 1 cut(s) 1804
PctI GAATGC 1 cut(s) 1375
PdmI GAANNNNTTC 3 cut(s) 512, 708, 834
PfeI GAWTC 6 cut(s) 161, 704, 1876, 2014, 2075, 2110
PflMI CCANNNNNTGG 2 cut(s) 22, 1341
PfoI TCCNGGA 1 cut(s) 433
PkrI GCNGC 7 cut(s) 328, 1041, 1236, 1290, 1524, 1527, 2129
PleI GAGTC 2 cut(s) 1653, 2155
PpsI GAGTC 2 cut(s) 1653, 2155
PpuMI RGGWCCY 1 cut(s) 1280
PscI ACATGT 1 cut(s) 1804
Psp124BI GAGCTC 2 cut(s) 81, 869
Psp5II RGGWCCY 1 cut(s) 1280
Psp6I CCWGG 1 cut(s) 433
PspGI CCWGG 1 cut(s) 433
PspN4I GGNNCC 5 cut(s) 1049, 1281, 1282, 1585, 1762
PspPI GGNCC 4 cut(s) 693, 785, 1280, 1787
PspPPI RGGWCCY 1 cut(s) 1280
PstI CTGCAG 2 cut(s) 1769, 1867
PsuI RGATCY 4 cut(s) 299, 883, 1583, 1694
PvuII CAGCTG 1 cut(s) 1873
RsaI GTAC 6 cut(s) 128, 186, 591, 1197, 1653, 2106
RsaNI GTAC 6 cut(s) 127, 185, 590, 1196, 1652, 2105
RseI CAYNNNNRTG 3 cut(s) 912, 935, 1160
SacI GAGCTC 2 cut(s) 81, 869
SaqAI TTAA 7 cut(s) 63, 320, 399, 1916, 2019, 2037, 2171
SatI GCNGC 7 cut(s) 327, 1040, 1235, 1289, 1523, 1526, 2128
Sau96I GGNCC 4 cut(s) 693, 785, 1280, 1787
SbfI CCTGCAGG 1 cut(s) 1769
ScaI AGTACT 1 cut(s) 2106
SchI GAGTC 2 cut(s) 1654, 2156
ScrFI CCNGG 1 cut(s) 435
SdaI CCTGCAGG 1 cut(s) 1769
SduI GDGCHC 3 cut(s) 81, 869, 1977
SfaNI GCATC 5 cut(s) 857, 1066, 1120, 1387, 1828
SfcI CTRYAG 2 cut(s) 1765, 1863
SinI GGWCC 4 cut(s) 693, 785, 1280, 1787
SmiMI CAYNNNNRTG 3 cut(s) 912, 935, 1160
SmlI CTYRAG 5 cut(s) 188, 398, 1517, 2018, 2036
SmoI CTYRAG 5 cut(s) 188, 398, 1517, 2018, 2036
Sse8387I CCTGCAGG 1 cut(s) 1769
SsiI CCGC 5 cut(s) 67, 957, 1320, 1852, 2128
SspMI CTAG 3 cut(s) 1112, 1451, 1545
SstI GAGCTC 2 cut(s) 81, 869
StyD4I CCNGG 1 cut(s) 433
StyI CCWWGG 2 cut(s) 852, 1111
TaaI ACNGT 4 cut(s) 937, 1666, 1787, 1979
TaiI ACGT 3 cut(s) 489, 1227, 1478
TaqI TCGA 4 cut(s) 312, 761, 2078, 2150
TatI WGTACW 4 cut(s) 126, 184, 1651, 2104
TauI GCSGC 1 cut(s) 2130
TfiI GAWTC 6 cut(s) 161, 704, 1876, 2014, 2075, 2110
Tru1I TTAA 7 cut(s) 63, 320, 399, 1916, 2019, 2037, 2171
Tru9I TTAA 7 cut(s) 63, 320, 399, 1916, 2019, 2037, 2171
TscAI CASTG 7 cut(s) 627, 930, 942, 1063, 1416, 1982, 1989
TseFI GTSAC 3 cut(s) 424, 520, 754
TseI GCWGC 6 cut(s) 326, 1039, 1234, 1288, 1522, 1525
Tsp45I GTSAC 3 cut(s) 424, 520, 754
TspDTI ATGAA 7 cut(s) 572, 585, 621, 780, 1008, 1382, 1868
TspRI CASTG 7 cut(s) 627, 930, 942, 1063, 1416, 1982, 1989
Van91I CCANNNNNTGG 2 cut(s) 22, 1341
Vha464I CTTAAG 3 cut(s) 398, 2018, 2036
VpaK11BI GGWCC 4 cut(s) 693, 785, 1280, 1787
XapI RAATTY 7 cut(s) 152, 497, 770, 1459, 1493, 2030, 2086
XceI RCATGY 1 cut(s) 1808
XmaJI CCTAGG 1 cut(s) 1111
XmiI GTMKAC 1 cut(s) 1668
XmnI GAANNNNTTC 3 cut(s) 512, 708, 834
XspI CTAG 3 cut(s) 1112, 1451, 1545
ZraI GACGTC 1 cut(s) 487
ZrmI AGTACT 1 cut(s) 2106
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.