Rw2G013230

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000039
Physical Location & Seq
Forward (+)
13971457 .. 13975981
4525 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G013230.1

Sequence Viewer

Length: 2529 bp
ATGGCGGCGAGCCTCGCTGCAGGTGGTGCTATGGGAGTACCATTTAATGCGCTCTATGAAGCCATTAAGCAAGTGGTGGTCAAGACTAGAATGTTTAAACCCCTTGTTGAAGACCTGAGGTCGAAGATAAACGCCTTGAAACCCCTCATCGATGCAATGGAGATCTACAATATAGCTCTGGGGATTTCAGAGGACGAACTTAGAGATTTCAAAATGCAAATGGAGAAGGGACCCAAGCTTATTCTCAAGTGTTCCAATCTTAGTGTATGGAAGACTTACAAAAAGTATAAATACAGTAACCAACTTCTTGAATTGCAGAACTCTCTTCAGAATCTGCTACTTGTACTGTCAGTGCAACAAGCAAGGGATGGGAAGGTGAACTTGGTTGCAACAAGGAATATAGAGAAGGTGGTGCAGCGAGTGGATGTGAATGTTGAGGCAATATTGAGACAACACCAAGAGATGAATGTTACGAATCAATTAAGAAGCATAAGCAGTACCGCGATCCAGCATACTGAAAACCAAGGTGAGGTACAATTTCGAATAGATTGTGAAGCACCTGTACCGCCAAGGGTTACAGTTGGATTGGAAGGGCCTTTGATGGAATTGAAGATGAAGCTTCTTAAGAATAATAATGTGTCAATGGTTGTGCTGACTGCTCCCGGAGGATCCGGGAAAACCACATTGGCCACAAAGTTTTGTCAAGATGAGGAAGTGAAAGATATATTCAAGAACAACATCTTCTTTGTCACTGTTTCAAAGAAGGCAAACTTCAACCTTATTGTACAAGGACTACATCAACGCAAGGGTACCGAAATACCTACTTTCGAAGAAGAAGCAACTGCCCTCAAGTGGTTGCATAAGTATCTGAAGGAAGAAGGACAGACACCTGTACTGTTGGTCCTGGATGATGTTTGGCCTGAATCAAAATCTCTTCTTGAAATTTTTGATGAAGTGAAAGGGCCGAATTTCAAGATTTTGGTGACATCAAGATCAAAATTTGCAGGATATGGTTCTCTATACCATCTAAAATCATTGAATGAGGCAGATGCGATGACTCTTTTCCGTAATTCAGCATCCCTGGGAGATGGGAGCTCTAGTGTTCAATGGACCAAACTTATTACAAAGATAGTAAAGCGCTGTAAGGGACATCCACTTGCCATTAAAGTGGTAGGAAAATCTCTTCGTGGACAATCTTTTGAGTTCTGGATGAAAAGAGAAATGGAGTGGTCTAAAGGTGATTCTATTATTGATTCTGAAACTGAAGTGCTTCGTTCCCTTCAAAGTAGCTTCGATGCCTTGGATGAAAAAGGAGCTATCATAAAGGAATGTTTTTTAGACCTTGGTTCATTTCCTGAAGACCAAAGAATTCCTGTTGCTGCTCTAATTGATATGTGGGCAGAGTTGCATGAGCTAGATAAAGATTTCCTGGCTGTTACACACCTGCAAGAGCTTACCACACGAAGTCTTGCTGATCTAGTCACAAGGAAAGATGCAGTGGAGGGTGATGAGTACTACAATGAACACTTCGTCACTCAACATGACTTGCTTAGAGAGCTGGCTATCTACCAAACCAAGTTAGATCCAAATAAACAAAGACTGATAATAGGCAACAATGGAGACGATCTACCCAAGTGCTTGAGAGAACAAACAGATCGACCCATCAAGCCTCGCCTATTATCTATCTCCTCTGATGGAGTGTTCTCAACAAGTTTGCACAACATTGAACTGGCAGAGGTTGAAGTTCTGGTTTTGAATTTCGACACAAAGGACTATGCATTTCCCGAATTTGTGGAGAAAATGGACAATCTGAAGGTTCTAATAGTCACAAATAATGGTTTCTTACCTGCTGAGCTGAGAAATTTTCACCTACTGGATTCTTTATCAAATCTGAAGAGAATCAGATTAGAGCGTGTTTCAATTCCTTCCATAACCAAGAACCCCCTACAGTTGAAGAGTCTAAAGAAGTTCTCGTTATTCATGTGTAGCATCGGAGAAGCTTTCAATTGTTCCTTCCAATTTTCACATGCATTTCCAAATTTAGAGGAAATGCATATTGACTATTGCAATGATTTGGTGGAATTACCTGATGAGCTATGCAATCTGGTTCGCCTAAACAAGCTCAGTATCACACACTGTCCTATTCTATCTGCCTTGCCTGAAAAGATTGGAGAGTTGGAAAACTTGGAAGTGTTGAGGCTAAGGTCCTGCACAGATTTAGCAGAATTGCCAAGCTCAACTACCAACCTCAAGAAGTTAAACTTTCTTGACATATCCTATTGCTTGAGCATCAAGGAACTGCCTAAACAGATTGGAGAAATGTGCAGTTTAATTAGGATCAATATGAGAGAATGCTCAAGATTGAAAGCACTGCCTATATCAATTTGTGATCTTGAGAATAATCTAGAGGAAGTGATATGCGATGACGAGACAGAAGAGTTGTGGGAGCCCTTCTTACCTAGACTGAACAAAGGAGTAGTAAGGGTTGTCGAGGAAGAATTCAACCTAAGCTGGCTCCAAAAGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

842

Amino Acids

95.7

Weight (kDa)

5.73

Isoelectric Point (pI)

38.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 3 - 132 2.8e-22 Arabidopsis broad-spectrum mildew resistance protein RPW8
NB-ARC PF00931 199 - 359 4e-17 NB-ARC domain
LRR_14 PF23598 676 - 784 6.5e-13 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000039)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66890 AT5G66900 AT5G66900 AT5G66900 AT5G66910
fragaria_vesca FvH4_1g15090 FvH4_1g15120 FvH4_1g15130 FvH4_1g15140 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15170 FvH4_1g15171 FvH4_1g15171 FvH4_1g15200 FvH4_1g15200 FvH4_1g15200 FvH4_1g15210 FvH4_1g15210 FvH4_1g15210 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15230 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15500 FvH4_1g15500 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_2g19760 FvH4_3g12820 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_6g18970 FvH4_6g42370 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g50100 FvH4_6g50100 FvH4_6g50110 FvH4_6g50130 FvH4_6g50140 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50330
malus_domestica MD00G1053700.v1.1 MD02G1164300.v1.1 MD02G1164400.v1.1 MD02G1164700.v1.1 MD02G1164800.v1.1 MD02G1165300.v1.1 MD02G1165400.v1.1 MD02G1165500.v1.1 MD02G1165600.v1.1 MD02G1165900.v1.1 MD02G1166000.v1.1 MD02G1166100.v1.1 MD02G1166300.v1.1 MD08G1084900.v1.1 MD15G1263100.v1.1 MD15G1263300.v1.1 MD15G1276900.v1.1 MD15G1277200.v1.1 MD15G1277400.v1.1 MD15G1277700.v1.1 MD15G1277800.v1.1 MD15G1278000.v1.1 MD15G1278100.v1.1 MD15G1278400.v1.1 MD15G1278600.v1.1
prunus_persica Prupe.7G138500_v2.0.a1 Prupe.7G138500_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138700_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138900_v2.0.a1 Prupe.7G139100_v2.0.a1 Prupe.7G139200_v2.0.a1 Prupe.7G139500_v2.0.a1 Prupe.7G139600_v2.0.a1 Prupe.7G139700_v2.0.a1 Prupe.7G139800_v2.0.a1
pyrus_communis pycom02g13150 pycom02g13160 pycom02g13190 pycom02g13200 pycom02g13220 pycom02g13230 pycom02g13250 pycom02g13260 pycom02g13270 pycom02g13300 pycom02g13310 pycom02g13330 pycom15g23980 pycom15g23990 pycom15g24030 pycom15g24040 pycom15g24050 pycom15g24070 pycom15g24090 pycom15g24160
rosa_chinensis RchiOBHm_Chr1g0326431 RchiOBHm_Chr1g0326451 RchiOBHm_Chr2g0104121 RchiOBHm_Chr2g0104151 RchiOBHm_Chr2g0104171 RchiOBHm_Chr2g0104181 RchiOBHm_Chr2g0104191 RchiOBHm_Chr2g0104201 RchiOBHm_Chr2g0104211 RchiOBHm_Chr2g0104241 RchiOBHm_Chr2g0104261 RchiOBHm_Chr2g0104281 RchiOBHm_Chr2g0104311 RchiOBHm_Chr2g0104331 RchiOBHm_Chr2g0104361 RchiOBHm_Chr2g0104381 RchiOBHm_Chr2g0104391 RchiOBHm_Chr2g0104431 RchiOBHm_Chr2g0104441 RchiOBHm_Chr2g0104541 RchiOBHm_Chr2g0104611 RchiOBHm_Chr2g0104741 RchiOBHm_Chr2g0104761 RchiOBHm_Chr2g0158291 RchiOBHm_Chr2g0170371 RchiOBHm_Chr2g0170631 RchiOBHm_Chr3g0473971 RchiOBHm_Chr3g0480491 RchiOBHm_Chr3g0480541 RchiOBHm_Chr3g0480561 RchiOBHm_Chr3g0485281 RchiOBHm_Chr3g0485291 RchiOBHm_Chr4g0411541 RchiOBHm_Chr6g0285471
rosa_laevigata RLG00000008347 RLG00000012637 RLG00000016961 RLG00000017293 RLG00000017305 RLG00000017308 RLG00000017312 RLG00000017314 RLG00000017317 RLG00000017318 RLG00000017319 RLG00000017321 RLG00000017322 RLG00000017323 RLG00000017324 RLG00000017325 RLG00000017327 RLG00000017328 RLG00000017329 RLG00000017330 RLG00000017334 RLG00000020833 RLG00000021073 RLG00000021950 RLG00000021971 RLG00000023154 RLG00000023157 RLG00000023467 RLG00000023468 RLG00000023525 RLG00000023963 RLG00000033265 RLG00000033267 RLG00000033268
rosa_multiflora Rmu_co8053082.1_g000001 Rmu_co8210358.1_g000001 Rmu_co8331661.1_g000001 Rmu_co8477427.1_g000002 Rmu_co8483217.1_g000001 Rmu_sc0000284.1_g000004 Rmu_sc0000676.1_g000017 Rmu_sc0000676.1_g000019 Rmu_sc0000676.1_g000021 Rmu_sc0002205.1_g000038 Rmu_sc0002205.1_g000041 Rmu_sc0002316.1_g000010 Rmu_sc0002316.1_g000012 Rmu_sc0002316.1_g000040 Rmu_sc0002316.1_g000071 Rmu_sc0002316.1_g000072 Rmu_sc0002316.1_g000095 Rmu_sc0002316.1_g000102 Rmu_sc0002414.1_g000018 Rmu_sc0002576.1_g000002 Rmu_sc0002576.1_g000003 Rmu_sc0002687.1_g000010 Rmu_sc0002687.1_g000017 Rmu_sc0003660.1_g000001 Rmu_sc0003787.1_g000014 Rmu_sc0003787.1_g000020 Rmu_sc0003787.1_g000024 Rmu_sc0003787.1_g000029 Rmu_sc0004059.1_g000026 Rmu_sc0005137.1_g000021 Rmu_sc0006550.1_g000001 Rmu_sc0006561.1_g000025 Rmu_sc0006561.1_g000033 Rmu_sc0007704.1_g000005 Rmu_sc0008904.1_g000005 Rmu_sc0010270.1_g000002 Rmu_sc0010307.1_g000001 Rmu_sc0011601.1_g000013 Rmu_sc0012694.1_g000005 Rmu_sc0013101.1_g000008 Rmu_sc0019029.1_g000001 Rmu_sc0019728.1_g000001 Rmu_sc0025829.1_g000001 Rmu_sc0025829.1_g000002 Rmu_ssc0000257.1_g000007 Rmu_ssc0000257.1_g000008
rosa_roxburghii Rroxscaffold_1G00021710 Rroxscaffold_1G00049350 Rroxscaffold_1G00049360 Rroxscaffold_2G00081160 Rroxscaffold_2G00081360 Rroxscaffold_2G00081390 Rroxscaffold_2G00091540 Rroxscaffold_2G00138790 Rroxscaffold_2G00138800 Rroxscaffold_2G00138920 Rroxscaffold_2G00139000 Rroxscaffold_2G00139010 Rroxscaffold_2G00139020 Rroxscaffold_2G00139030 Rroxscaffold_2G00139040 Rroxscaffold_2G00139050 Rroxscaffold_2G00139060 Rroxscaffold_2G00139070 Rroxscaffold_2G00139090 Rroxscaffold_2G00139100 Rroxscaffold_3G00257470 Rroxscaffold_4G00318040 Rroxscaffold_4G00323740 Rroxscaffold_4G00323750 Rroxscaffold_5G00356140 Rroxscaffold_6G00401280 Rroxscaffold_6G00401290 Rroxscaffold_7G00183120
rosa_rugosa Rorug01G0060500 Rorug01G0060900 Rorug02G0119500 Rorug02G0119600 Rorug02G0119700 Rorug02G0119700 Rorug02G0119700 Rorug02G0119800.1 Rorug02G0119900.1 Rorug02G0120000 Rorug02G0120100 Rorug02G0120200 Rorug02G0120300 Rorug02G0120400 Rorug02G0120500 Rorug02G0120700 Rorug02G0120700 Rorug02G0122300 Rorug02G0122300 Rorug02G0122400.1 Rorug02G0466700 Rorug02G0466700 Rorug02G0466800 Rorug02G0548600 Rorug02G0549900 Rorug02G0550000 Rorug03G0184200 Rorug04G0107900 Rorug05G0121500 Rorug06G0174800
rosa_samantha Rh1AG198400 Rh1CG074700 Rh1CG075000 Rh1CG183700 Rh2AG169700 Rh2AG169800 Rh2AG169900 Rh2AG170000 Rh2AG170300 Rh2AG170400 Rh2AG171000 Rh2AG171100 Rh2AG171600 Rh2AG172400 Rh2AG172500 Rh2AG532900 Rh2AG620000 Rh2AG622500 Rh2AG622800 Rh2AG623000 Rh2BG176200 Rh2BG176300 Rh2BG176400 Rh2BG176500 Rh2BG176600 Rh2BG176900 Rh2BG177000 Rh2BG177100 Rh2BG177200 Rh2BG177300 Rh2BG177600 Rh2BG177700 Rh2BG177900 Rh2BG178000 Rh2BG178200 Rh2BG178300 Rh2BG178500 Rh2BG179000 Rh2BG180000 Rh2BG180100 Rh2BG259200 Rh2BG546700 Rh2BG630800 Rh2BG633500 Rh2CG176500 Rh2CG177500 Rh2CG177700 Rh2DG174400 Rh2DG174600 Rh2DG174900 Rh2DG175100 Rh2DG175200 Rh2DG175300 Rh2DG175600 Rh2DG175700 Rh2DG175800 Rh2DG175900 Rh2DG176000 Rh2DG176300 Rh2DG176400 Rh2DG176500 Rh2DG176600 Rh2DG176900 Rh2DG177100 Rh2DG177200 Rh2DG177800 Rh2DG177900 Rh2DG178700 Rh2DG178800 Rh2DG555700 Rh2DG643700 Rh2DG646300 Rh3AG235600 Rh3AG235900 Rh3AG236200 Rh3AG266700 Rh3AG266900 Rh3BG216200 Rh3BG269100 Rh3CG212700 Rh3CG265800 Rh4AG168900 Rh4BG165500 Rh4CG180300 Rh5BG261500 Rh6AG286300 Rh6BG287500 Rh6CG288600 Rh7AG022600
rosa_wichuraiana Rw0G008240 Rw0G008250 Rw0G013190 Rw0G013200 Rw2G013230 Rw2G013240 Rw2G013270 Rw2G013280 Rw2G013290 Rw2G013300 Rw2G013330 Rw2G013340 Rw2G013350 Rw2G013360 Rw2G013370 Rw2G013380 Rw2G013410 Rw2G013570 Rw2G044120 Rw2G051370 Rw2G051590 Rw3G017160 Rw3G021150 Rw3G021160 Rw3G023730 Rw3G023740 Rw4G014030 Rw6G024590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 11, 1452
Acc36I ACCTGC 3 cut(s) 11, 1452, 1855
Acc65I GGTACC 1 cut(s) 809
AccB1I GGYRCC 1 cut(s) 809
AccII CGCG 1 cut(s) 503
AciI CCGC 3 cut(s) 5, 501, 566
AclWI GGATC 5 cut(s) 499, 663, 676, 1577, 2345
AcoI YGGCCR 1 cut(s) 687
AcsI RAATTY 9 cut(s) 942, 967, 998, 1368, 1756, 1787, 1861, 2038, 2498
AcuI CTGAAG 6 cut(s) 311, 890, 1284, 1377, 1832, 1913
AfaI GTAC 9 cut(s) 39, 345, 499, 534, 564, 786, 811, 894, 1514
AfeI AGCGCT 1 cut(s) 1139
AfiI CCNNNNNNNGG 2 cut(s) 529, 852
AflII CTTAAG 1 cut(s) 623
AhdI GACNNNNNGTC 1 cut(s) 118
AjnI CCWGG 3 cut(s) 903, 1080, 1428
AjuI GAANNNNNNNTTGG 4 cut(s) 365, 397, 668, 700
Alw21I GWGCWC 1 cut(s) 1097
Alw26I GTCTC 3 cut(s) 442, 1614, 2423
AlwI GGATC 5 cut(s) 499, 663, 676, 1577, 2345
AlwNI CAGNNNCTG 1 cut(s) 334
Aor51HI AGCGCT 1 cut(s) 1139
AoxI GGCC 4 cut(s) 593, 687, 917, 962
ApeKI GCWGC 3 cut(s) 17, 415, 1379
ApoI RAATTY 9 cut(s) 942, 967, 998, 1368, 1756, 1787, 1861, 2038, 2498
Asp700I GAANNNNTTC 1 cut(s) 1269
Asp718I GGTACC 1 cut(s) 809
AspLEI GCGC 2 cut(s) 52, 1140
AspS9I GGNCC 6 cut(s) 230, 593, 901, 962, 1110, 2205
AsuC2I CCSGG 2 cut(s) 663, 673
AsuHPI GGTGA 6 cut(s) 388, 539, 994, 1250, 1517, 1859
AsuII TTCGAA 2 cut(s) 541, 828
AvaII GGWCC 4 cut(s) 230, 901, 1110, 2205
AxyI CCTNAGG 1 cut(s) 116
BalI TGGCCA 1 cut(s) 689
BamHI GGATCC 1 cut(s) 668
BanI GGYRCC 1 cut(s) 809
BanII GRGCYC 2 cut(s) 1097, 2451
BarI GAAGNNNNNNTAC 2 cut(s) 546, 578
BbsI GAAGAC 3 cut(s) 117, 278, 1365
Bbv12I GWGCWC 1 cut(s) 1097
BbvI GCAGC 3 cut(s) 4, 427, 1366
BccI CCATC 6 cut(s) 362, 595, 1032, 1082, 1670, 1688
BciT130I CCWGG 3 cut(s) 905, 1082, 1430
BcnI CCSGG 2 cut(s) 663, 673
BcoDI GTCTC 3 cut(s) 442, 1614, 2423
BfaI CTAG 6 cut(s) 87, 1098, 1415, 1478, 2405, 2460
BfmI CTRYAG 2 cut(s) 18, 1946
BfoI RGCGCY 1 cut(s) 1141
BfrI CTTAAG 1 cut(s) 623
BfuAI ACCTGC 3 cut(s) 11, 1452, 1855
BglII AGATCT 1 cut(s) 162
BisI GCNGC 4 cut(s) 6, 18, 416, 1380
BlpI GCTNAGC 1 cut(s) 1851
BlsI GCNGC 4 cut(s) 7, 19, 417, 1381
BmcAI AGTACT 1 cut(s) 1514
Bme1390I CCNGG 5 cut(s) 663, 673, 905, 1082, 1430
Bme18I GGWCC 4 cut(s) 230, 901, 1110, 2205
BmeRI GACNNNNNGTC 1 cut(s) 118
BmgT120I GGNCC 6 cut(s) 230, 593, 901, 962, 1110, 2205
BmiI GGNNCC 6 cut(s) 231, 232, 670, 811, 2448, 2516
BmrFI CCNGG 5 cut(s) 663, 673, 905, 1082, 1430
BmsI GCATC 7 cut(s) 142, 1039, 1085, 1285, 1483, 1998, 2298
BpiI GAAGAC 3 cut(s) 117, 278, 1365
Bpu10I CCTNAGC 2 cut(s) 2201, 2507
Bpu1102I GCTNAGC 1 cut(s) 1851
Bpu14I TTCGAA 2 cut(s) 541, 828
BpuEI CTTGAG 7 cut(s) 230, 833, 1660, 2234, 2305, 2341, 2414
BpuMI CCSGG 2 cut(s) 663, 673
Bsa29I ATCGAT 1 cut(s) 150
BsaJI CCNNGG 6 cut(s) 523, 569, 1080, 1081, 1299, 1342
BsaXI ACNNNNNCTCC 2 cut(s) 1084, 1114
Bsc4I CCNNNNNNNGG 2 cut(s) 529, 852
Bse1I ACTGG 2 cut(s) 1734, 1878
Bse21I CCTNAGG 1 cut(s) 116
Bse3DI GCAATG 2 cut(s) 162, 2074
BseBI CCWGG 3 cut(s) 905, 1082, 1430
BseCI ATCGAT 1 cut(s) 150
BseDI CCNNGG 6 cut(s) 523, 569, 1080, 1081, 1299, 1342
BseGI GGATG 7 cut(s) 373, 430, 913, 1076, 1150, 1215, 1309
BseLI CCNNNNNNNGG 2 cut(s) 529, 852
BseMI GCAATG 2 cut(s) 162, 2074
BseMII CTCAG 4 cut(s) 107, 1842, 1847, 2137
BseNI ACTGG 2 cut(s) 1734, 1878
BseRI GAGGAG 1 cut(s) 1678
BseXI GCAGC 3 cut(s) 4, 427, 1366
BsgI GTGCAG 3 cut(s) 434, 2194, 2344
Bsh1236I CGCG 1 cut(s) 503
BshFI GGCC 4 cut(s) 595, 689, 919, 964
BshNI GGYRCC 1 cut(s) 809
BshVI ATCGAT 1 cut(s) 150
BsiHKAI GWGCWC 1 cut(s) 1097
BsiSI CCGG 2 cut(s) 663, 672
BslFI GGGAC 2 cut(s) 243, 1161
BslI CCNNNNNNNGG 2 cut(s) 529, 852
BsmAI GTCTC 3 cut(s) 442, 1614, 2423
BsmBI CGTCTC 1 cut(s) 1614
BsmFI GGGAC 2 cut(s) 243, 1161
BsmI GAATGC 1 cut(s) 2357
BsnI GGCC 4 cut(s) 595, 689, 919, 964
Bsp119I TTCGAA 2 cut(s) 541, 828
Bsp1286I GDGCHC 2 cut(s) 1097, 2451
Bsp1407I TGTACA 1 cut(s) 784
Bsp1720I GCTNAGC 1 cut(s) 1851
BspACI CCGC 3 cut(s) 5, 501, 566
BspANI GGCC 4 cut(s) 595, 689, 919, 964
BspCNI CTCAG 4 cut(s) 108, 1843, 1848, 2136
BspDI ATCGAT 1 cut(s) 150
BspFNI CGCG 1 cut(s) 503
BspLI GGNNCC 6 cut(s) 231, 232, 670, 811, 2448, 2516
BspMAI CTGCAG 1 cut(s) 22
BspMI ACCTGC 3 cut(s) 11, 1452, 1855
BspPI GGATC 5 cut(s) 499, 663, 676, 1577, 2345
BspT104I TTCGAA 2 cut(s) 541, 828
BspT107I GGYRCC 1 cut(s) 809
BspTI CTTAAG 1 cut(s) 623
BsrDI GCAATG 2 cut(s) 162, 2074
BsrGI TGTACA 1 cut(s) 784
BsrI ACTGG 2 cut(s) 1734, 1878
BssECI CCNNGG 6 cut(s) 523, 569, 1080, 1081, 1299, 1342
BssT1I CCWWGG 4 cut(s) 523, 569, 1299, 1342
Bst2UI CCWGG 3 cut(s) 905, 1082, 1430
Bst4CI ACNGT 7 cut(s) 296, 348, 580, 754, 897, 1950, 2138
Bst6I CTCTTC 6 cut(s) 330, 939, 1188, 1889, 1949, 2430
BstAFI CTTAAG 1 cut(s) 623
BstAPI GCANNNNNTGC 1 cut(s) 26
BstAUI TGTACA 1 cut(s) 784
BstBI TTCGAA 2 cut(s) 541, 828
BstC8I GCNNGC 3 cut(s) 10, 1560, 2513
BstDEI CTNAG 9 cut(s) 116, 200, 260, 1550, 1851, 1856, 2123, 2201, 2507
BstF5I GGATG 7 cut(s) 373, 430, 913, 1076, 1150, 1215, 1309
BstFNI CGCG 1 cut(s) 503
BstH2I RGCGCY 1 cut(s) 1141
BstHHI GCGC 2 cut(s) 52, 1140
BstMAI GTCTC 3 cut(s) 442, 1614, 2423
BstMWI GCNNNNNNNGC 3 cut(s) 14, 26, 1555
BstNI CCWGG 3 cut(s) 905, 1082, 1430
BstNSI RCATGY 1 cut(s) 2030
BstSCI CCNGG 5 cut(s) 661, 671, 903, 1080, 1428
BstSFI CTRYAG 2 cut(s) 18, 1946
BstUI CGCG 1 cut(s) 503
BstV1I GCAGC 3 cut(s) 4, 427, 1366
BstV2I GAAGAC 3 cut(s) 117, 278, 1365
BstX2I RGATCY 3 cut(s) 162, 668, 1582
BstXI CCANNNNNNTGG 1 cut(s) 1168
BstYI RGATCY 3 cut(s) 162, 668, 1582
Bsu15I ATCGAT 1 cut(s) 150
Bsu36I CCTNAGG 1 cut(s) 116
BsuRI GGCC 4 cut(s) 595, 689, 919, 964
BsuTUI ATCGAT 1 cut(s) 150
BtgZI GCGATG 2 cut(s) 1067, 2436
BtsCI GGATG 7 cut(s) 373, 430, 913, 1076, 1150, 1215, 1309
BtsI GCAGTG 2 cut(s) 1503, 2369
BtsIMutI CAGTG 5 cut(s) 357, 750, 1503, 2134, 2369
BveI ACCTGC 3 cut(s) 11, 1452, 1855
Cac8I GCNNGC 3 cut(s) 10, 1560, 2513
CaiI CAGNNNCTG 1 cut(s) 334
CfoI GCGC 2 cut(s) 52, 1140
Cfr13I GGNCC 6 cut(s) 230, 593, 901, 962, 1110, 2205
ClaI ATCGAT 1 cut(s) 150
Csp6I GTAC 9 cut(s) 38, 344, 498, 533, 563, 785, 810, 893, 1513
CviAII CATG 4 cut(s) 1409, 1541, 1981, 2027
CviQI GTAC 9 cut(s) 38, 344, 498, 533, 563, 785, 810, 893, 1513
DdeI CTNAG 9 cut(s) 116, 200, 260, 1550, 1851, 1856, 2123, 2201, 2507
DraI TTTAAA 1 cut(s) 97
DriI GACNNNNNGTC 1 cut(s) 118
EaeI YGGCCR 1 cut(s) 687
Eam1104I CTCTTC 6 cut(s) 330, 939, 1188, 1889, 1949, 2430
Eam1105I GACNNNNNGTC 1 cut(s) 118
EarI CTCTTC 6 cut(s) 330, 939, 1188, 1889, 1949, 2430
Ecl136II GAGCTC 1 cut(s) 1095
Eco130I CCWWGG 4 cut(s) 523, 569, 1299, 1342
Eco24I GRGCYC 2 cut(s) 1097, 2451
Eco47I GGWCC 4 cut(s) 230, 901, 1110, 2205
Eco47III AGCGCT 1 cut(s) 1139
Eco53kI GAGCTC 1 cut(s) 1095
Eco57I CTGAAG 6 cut(s) 311, 890, 1284, 1377, 1832, 1913
Eco81I CCTNAGG 1 cut(s) 116
EcoICRI GAGCTC 1 cut(s) 1095
EcoO109I RGGNCCY 3 cut(s) 230, 593, 2205
EcoRI GAATTC 2 cut(s) 1368, 2498
EcoRII CCWGG 3 cut(s) 903, 1080, 1428
EcoT14I CCWWGG 4 cut(s) 523, 569, 1299, 1342
EcoT22I ATGCAT 3 cut(s) 1780, 2032, 2055
EcoT38I GRGCYC 2 cut(s) 1097, 2451
ErhI CCWWGG 4 cut(s) 523, 569, 1299, 1342
Esp3I CGTCTC 1 cut(s) 1614
FaeI CATG 4 cut(s) 1412, 1544, 1984, 2030
FalI AAGNNNNNCTT 6 cut(s) 365, 397, 755, 787, 2246, 2278
FaqI GGGAC 2 cut(s) 243, 1161
FatI CATG 4 cut(s) 1408, 1540, 1980, 2026
Fnu4HI GCNGC 4 cut(s) 6, 18, 416, 1380
FokI GGATG 7 cut(s) 380, 437, 920, 1063, 1137, 1222, 1316
FriOI GRGCYC 2 cut(s) 1097, 2451
Fsp4HI GCNGC 4 cut(s) 6, 18, 416, 1380
FspBI CTAG 6 cut(s) 87, 1098, 1415, 1478, 2405, 2460
GlaI GCGC 2 cut(s) 51, 1139
GluI GCNGC 4 cut(s) 6, 18, 416, 1380
HaeII RGCGCY 1 cut(s) 1141
HaeIII GGCC 4 cut(s) 595, 689, 919, 964
HapII CCGG 2 cut(s) 663, 672
HhaI GCGC 2 cut(s) 52, 1140
Hin1II CATG 4 cut(s) 1412, 1544, 1984, 2030
Hin6I GCGC 2 cut(s) 50, 1138
HinP1I GCGC 2 cut(s) 50, 1138
HindIII AAGCTT 3 cut(s) 236, 617, 1998
HinfI GANTC 9 cut(s) 331, 475, 923, 1057, 1241, 1253, 1877, 1899, 1957
HpaII CCGG 2 cut(s) 663, 672
HphI GGTGA 6 cut(s) 388, 539, 994, 1250, 1517, 1859
Hpy166II GTNNAC 2 cut(s) 379, 1190
Hpy188I TCNGA 9 cut(s) 190, 330, 870, 1258, 1693, 1812, 1893, 1904, 1994
Hpy8I GTNNAC 2 cut(s) 379, 1190
HpyCH4III ACNGT 7 cut(s) 296, 348, 580, 754, 897, 1950, 2138
HpyF10VI GCNNNNNNNGC 3 cut(s) 14, 26, 1555
HpyF3I CTNAG 9 cut(s) 116, 200, 260, 1550, 1851, 1856, 2123, 2201, 2507
Hsp92II CATG 4 cut(s) 1412, 1544, 1984, 2030
HspAI GCGC 2 cut(s) 50, 1138
KflI GGGWCCC 1 cut(s) 230
KpnI GGTACC 1 cut(s) 813
LmnI GCTCC 5 cut(s) 664, 1092, 1313, 2446, 2520
Lsp1109I GCAGC 3 cut(s) 4, 427, 1366
LweI GCATC 7 cut(s) 142, 1039, 1085, 1285, 1483, 1998, 2298
MaeI CTAG 6 cut(s) 87, 1098, 1415, 1478, 2405, 2460
MaeIII GTNAC 9 cut(s) 296, 469, 574, 748, 982, 1435, 1480, 1531, 1825
MfeI CAATTG 1 cut(s) 2005
MflI RGATCY 3 cut(s) 162, 668, 1582
MhlI GDGCHC 2 cut(s) 1097, 2451
MlsI TGGCCA 1 cut(s) 689
MluNI TGGCCA 1 cut(s) 689
MlyI GAGTC 2 cut(s) 1051, 1966
MmeI TCCRAC 2 cut(s) 562, 2157
Mox20I TGGCCA 1 cut(s) 689
Mph1103I ATGCAT 3 cut(s) 1780, 2032, 2055
MroXI GAANNNNTTC 1 cut(s) 1269
MscI TGGCCA 1 cut(s) 689
MseI TTAA 8 cut(s) 45, 66, 96, 482, 624, 1164, 2258, 2330
MslI CAYNNNNRTG 1 cut(s) 1166
Msp20I TGGCCA 1 cut(s) 689
MspCI CTTAAG 1 cut(s) 623
MspI CCGG 2 cut(s) 663, 672
MspR9I CCNGG 5 cut(s) 663, 673, 905, 1082, 1430
MssI GTTTAAAC 1 cut(s) 97
MunI CAATTG 1 cut(s) 2005
Mva1269I GAATGC 1 cut(s) 2357
MvaI CCWGG 3 cut(s) 905, 1082, 1430
MvnI CGCG 1 cut(s) 503
MwoI GCNNNNNNNGC 3 cut(s) 14, 26, 1555
NciI CCSGG 2 cut(s) 663, 673
NlaIII CATG 4 cut(s) 1412, 1544, 1984, 2030
NlaIV GGNNCC 6 cut(s) 231, 232, 670, 811, 2448, 2516
NmuCI GTSAC 5 cut(s) 748, 982, 1480, 1531, 1825
NsiI ATGCAT 3 cut(s) 1780, 2032, 2055
NspI RCATGY 1 cut(s) 2030
NspV TTCGAA 2 cut(s) 541, 828
PaqCI CACCTGC 2 cut(s) 11, 1452
PasI CCCWGGG 1 cut(s) 1081
PctI GAATGC 1 cut(s) 2357
PdmI GAANNNNTTC 1 cut(s) 1269
PfeI GAWTC 7 cut(s) 331, 475, 923, 1241, 1253, 1877, 1899
PfoI TCCNGGA 3 cut(s) 661, 671, 903
PkrI GCNGC 4 cut(s) 7, 19, 417, 1381
PleI GAGTC 2 cut(s) 1051, 1965
PmeI GTTTAAAC 1 cut(s) 97
PpsI GAGTC 2 cut(s) 1051, 1965
PpuMI RGGWCCY 2 cut(s) 230, 2205
Psp124BI GAGCTC 1 cut(s) 1097
Psp5II RGGWCCY 2 cut(s) 230, 2205
Psp6I CCWGG 3 cut(s) 903, 1080, 1428
PspGI CCWGG 3 cut(s) 903, 1080, 1428
PspN4I GGNNCC 6 cut(s) 231, 232, 670, 811, 2448, 2516
PspPI GGNCC 6 cut(s) 230, 593, 901, 962, 1110, 2205
PspPPI RGGWCCY 2 cut(s) 230, 2205
PstI CTGCAG 1 cut(s) 22
PstNI CAGNNNCTG 1 cut(s) 334
PsuI RGATCY 3 cut(s) 162, 668, 1582
RsaI GTAC 9 cut(s) 39, 345, 499, 534, 564, 786, 811, 894, 1514
RsaNI GTAC 9 cut(s) 38, 344, 498, 533, 563, 785, 810, 893, 1513
RseI CAYNNNNRTG 1 cut(s) 1166
SacI GAGCTC 1 cut(s) 1097
SaqAI TTAA 8 cut(s) 45, 66, 96, 482, 624, 1164, 2258, 2330
SatI GCNGC 4 cut(s) 6, 18, 416, 1380
Sau96I GGNCC 6 cut(s) 230, 593, 901, 962, 1110, 2205
ScaI AGTACT 1 cut(s) 1514
SchI GAGTC 2 cut(s) 1051, 1966
ScrFI CCNGG 5 cut(s) 663, 673, 905, 1082, 1430
SduI GDGCHC 2 cut(s) 1097, 2451
SfaNI GCATC 7 cut(s) 142, 1039, 1085, 1285, 1483, 1998, 2298
SfcI CTRYAG 2 cut(s) 18, 1946
SfuI TTCGAA 2 cut(s) 541, 828
SinI GGWCC 4 cut(s) 230, 901, 1110, 2205
SmiMI CAYNNNNRTG 1 cut(s) 1166
SmlI CTYRAG 8 cut(s) 245, 623, 848, 1639, 2249, 2284, 2356, 2393
SmoI CTYRAG 8 cut(s) 245, 623, 848, 1639, 2249, 2284, 2356, 2393
SsiI CCGC 3 cut(s) 5, 501, 566
SspI AATATT 1 cut(s) 444
SspMI CTAG 6 cut(s) 87, 1098, 1415, 1478, 2405, 2460
SstI GAGCTC 1 cut(s) 1097
StyD4I CCNGG 5 cut(s) 661, 671, 903, 1080, 1428
StyI CCWWGG 4 cut(s) 523, 569, 1299, 1342
TaaI ACNGT 7 cut(s) 296, 348, 580, 754, 897, 1950, 2138
TaqI TCGA 8 cut(s) 122, 150, 541, 828, 1293, 1657, 1761, 2490
TatI WGTACW 4 cut(s) 343, 784, 892, 1512
TauI GCSGC 1 cut(s) 8
TfiI GAWTC 7 cut(s) 331, 475, 923, 1241, 1253, 1877, 1899
Tru1I TTAA 8 cut(s) 45, 66, 96, 482, 624, 1164, 2258, 2330
Tru9I TTAA 8 cut(s) 45, 66, 96, 482, 624, 1164, 2258, 2330
TscAI CASTG 5 cut(s) 357, 757, 1503, 2141, 2376
TseFI GTSAC 5 cut(s) 748, 982, 1480, 1531, 1825
TseI GCWGC 3 cut(s) 17, 415, 1379
Tsp45I GTSAC 5 cut(s) 748, 982, 1480, 1531, 1825
TspDTI ATGAA 9 cut(s) 72, 479, 629, 966, 1226, 1320, 1338, 1536, 1969
TspGWI ACGGA 1 cut(s) 1055
TspRI CASTG 5 cut(s) 357, 757, 1503, 2141, 2376
Vha464I CTTAAG 1 cut(s) 623
VpaK11BI GGWCC 4 cut(s) 230, 901, 1110, 2205
XapI RAATTY 9 cut(s) 942, 967, 998, 1368, 1756, 1787, 1861, 2038, 2498
XbaI TCTAGA 1 cut(s) 2404
XceI RCATGY 1 cut(s) 2030
XcmI CCANNNNNNNNNTGG 1 cut(s) 70
XmnI GAANNNNTTC 1 cut(s) 1269
XspI CTAG 6 cut(s) 87, 1098, 1415, 1478, 2405, 2460
ZrmI AGTACT 1 cut(s) 1514
Zsp2I ATGCAT 3 cut(s) 1780, 2032, 2055
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.