MD15G1278100.v1.1

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000039
Physical Location & Seq
Forward (+)
24567227 .. 24570934
3708 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1278100.v1.1.491

Sequence Viewer

Length: 2451 bp
ATGTCTCTAGCTTCAGTCGGAATAGGTGCTCTCAGTACGCCATTTCAAATGCTGTTTGATGCTGTAAAATCAGCGCAGCCGGAGAATAAAATGCTCCGGCACCGCCTCCGAGACATCAAATCCACACTAGACTCTCTACAACCACTCATCATAGACATCGAAAAATCTGACCCCAAAGAGCAACTGCAACATTTTGCACTACAGATGGAGGAGGGGGCAAATCTTGTTCACAAGTGCACCAAAAATCGTCTTCAGAAGTGCAGCAAAGTTGCCCTGTGGAGAAAATACATATACGCCAAAAAGCTTCGTCAACTGGACAAATCTCTTCGAAGAGTGTTTGATGTTCTTAAACTGCAGGGTACGAGGGATGCGAGGGAGACGTTGGTTTCTTTGAGGATTATCGAGAAGGTGCTCTCTCGAGTAGAAGAGAGTTTGAAGATGAAAACTAATCAGCCTGAAATAGTTACATGTTCGCCTAGGGTGCCTGAAGCGCCATCGGTTGCAGTCGGGTTGGATGTGCCGTTGAAGGAGTTGAAGATGAAGCTCCTCAAGGATGAAAAGGTGTCTATGCTTGTGCTGACTGCTGCTGGAGGGTGTGGGAAAACCACCTTGGCTAAAAAGTTTTGTCAGGATCAAGAAGTCAAAGATACATTCAAGAAGAACATCTTCTTTGTCAATGTCTCGAAGAAGCCCAACTTGGGCCTTATTGTACATGAACTATATCAACAGAAGGGGACCGAGCTGCCTGCTGTCCAAAACGAAGTCATGGCAGCTAACCGTTTGCAACAATTTCTCAAGGAATCAACACAAAATCCTCTACTATTTGTCCTGGATGATGTTTGGTCGGGATCAGAGCCCCTTCTTGAGAAGTTTGATCAATTGAAGTTTTCAAATTACAAGATTTTGGTCACATCAAGATTTGCATTTCCAAGATTCGGTTCTCCTTATCGTTTGGCATCATTGAGTGATGAAGATGCAATGATCCTTTTTCACTACTCGGCATCCTTGGTGGATAAGAGCTCTTATACTTACGAAGATCTTTCGAGAAAGATTATCAAGCGCTGTAAGGGATGCCCACTGGCCATTGCATTGGTCGGGAGATCACTTCGCGGCCAGCCTATAGAAATCTGGCACAAAAGAGTAGTGGAATGGTGCAAAGGTTCATCTATTCTTGATTCTGACAAGGAATTGCTTGCTTGCCTCCTAAGCAGTTTAGATGCCTTGGATAAAGAAAAAGCTATCATCAAGGAATGTTTCCTCGATCTAGGTTCTTTTCCGGAGGACCAAAGAATTGCTGTTGCCGCCCTCATTGATATGTGGGCAGAGTTATATGATCTAGATGAAGAGATTCTGACCATTGCCAATCTTCACGAGCTCACCGCCCGAAGTTTAGCTAATCTTGTTGTCACAAGGCATGAGATGGGAGCGGATGGCTATTACACTGAACACTTCGTCACCCAGCACGACATGCTTAGAGAGTTGGCGATCCATCAGGCGAGTCAAGGCCCAATAGAACATAGGGAACGACTATTTATAAACATATGTGGGGACAAACTTCCCAAGTGGTTGACAGAACAAAAGTATCAAGCGATCAAGGCTCGACTATTATCTATCTCAACAGATGGAGCCTTCTCGGCAAAATGGCCAAACATGCAAGTACCCGAGGCAGAGGTTCTAGTTCTGAATTTTCAAACGAGGAACTATGCCTTACCTGAGTTTGTGGAGAAAATGGATAAACTAAAGGTTCTAATACTAAAAAATGACGGTTTGTTACCTGCTGAACTAGATAATTTTGAACTACTTGGTTCGCTATCAAATCTTAAGAGAATTAGATTAGAACGTATTTCAATTCCTCCCATAAGCAAGGTCCTTAAACAGTTGAAAAGCTTGCAAAAGATCTCTCTTTTCATGTGTGATATCGGCCAAGGTTTCGGCCAAATTTTAGATGCACTGCCAAATCTAGTGGAATTGAACATTGATTATTGCCATGATCTGGTGCATCTTCCTGACTACGTTTGTGACCTTGTTCACCTTAAGAAGCTTAGCATCACCAACTGTCATAAGCTCTCTGCCTTGCCTGCAGACATCGGAAAGCTAGTCAATCTTGAAGTGTTGAGGCTTAGGTCATGTGCAGAGTTGTTAGAGTTGCCAGGCTCGATTAGGAACCTTAAAGTTTTGAAATTTCTCGACATATCCGGTTGCTTTAGCGTAAAGGAGTTGCCCGAGGACATTGGTGCAATGGGCAGCATGGAAAAGATCAACGTGAGCCAGTGTTCGAGACTGAAAGAGCTGCCTGCTTCTGTTATGGATCTTGAACAGTTGAACGAAGTGATATGCGACGAGGAGACGAAAACTTTATGGGAACCCTTCTTACCGTTTCTCACAAACATACACATAAAGGTGATCAAAGAAGATATCAACCTAAATTGGCTCAACAAGCTTCCATCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

817

Amino Acids

92.6

Weight (kDa)

7.95

Isoelectric Point (pI)

45.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 10 - 132 2.6e-19 Arabidopsis broad-spectrum mildew resistance protein RPW8
NB-ARC PF00931 175 - 306 8.1e-15 NB-ARC domain
AAA_22 PF13401 189 - 294 4.7e-07 AAA domain
LRR_14 PF23598 657 - 732 4.2e-09 Leucine-rich repeat region
LRR_14 PF23598 720 - 784 1.2e-07 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000039)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66890 AT5G66900 AT5G66900 AT5G66900 AT5G66910
fragaria_vesca FvH4_1g15090 FvH4_1g15120 FvH4_1g15130 FvH4_1g15140 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15170 FvH4_1g15171 FvH4_1g15171 FvH4_1g15200 FvH4_1g15200 FvH4_1g15200 FvH4_1g15210 FvH4_1g15210 FvH4_1g15210 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15230 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15500 FvH4_1g15500 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_2g19760 FvH4_3g12820 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_6g18970 FvH4_6g42370 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g50100 FvH4_6g50100 FvH4_6g50110 FvH4_6g50130 FvH4_6g50140 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50330
malus_domestica MD00G1053700.v1.1 MD02G1164300.v1.1 MD02G1164400.v1.1 MD02G1164700.v1.1 MD02G1164800.v1.1 MD02G1165300.v1.1 MD02G1165400.v1.1 MD02G1165500.v1.1 MD02G1165600.v1.1 MD02G1165900.v1.1 MD02G1166000.v1.1 MD02G1166100.v1.1 MD02G1166300.v1.1 MD08G1084900.v1.1 MD15G1263100.v1.1 MD15G1263300.v1.1 MD15G1276900.v1.1 MD15G1277200.v1.1 MD15G1277400.v1.1 MD15G1277700.v1.1 MD15G1277800.v1.1 MD15G1278000.v1.1 MD15G1278100.v1.1 MD15G1278400.v1.1 MD15G1278600.v1.1
prunus_persica Prupe.7G138500_v2.0.a1 Prupe.7G138500_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138700_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138900_v2.0.a1 Prupe.7G139100_v2.0.a1 Prupe.7G139200_v2.0.a1 Prupe.7G139500_v2.0.a1 Prupe.7G139600_v2.0.a1 Prupe.7G139700_v2.0.a1 Prupe.7G139800_v2.0.a1
pyrus_communis pycom02g13150 pycom02g13160 pycom02g13190 pycom02g13200 pycom02g13220 pycom02g13230 pycom02g13250 pycom02g13260 pycom02g13270 pycom02g13300 pycom02g13310 pycom02g13330 pycom15g23980 pycom15g23990 pycom15g24030 pycom15g24040 pycom15g24050 pycom15g24070 pycom15g24090 pycom15g24160
rosa_chinensis RchiOBHm_Chr1g0326431 RchiOBHm_Chr1g0326451 RchiOBHm_Chr2g0104121 RchiOBHm_Chr2g0104151 RchiOBHm_Chr2g0104171 RchiOBHm_Chr2g0104181 RchiOBHm_Chr2g0104191 RchiOBHm_Chr2g0104201 RchiOBHm_Chr2g0104211 RchiOBHm_Chr2g0104241 RchiOBHm_Chr2g0104261 RchiOBHm_Chr2g0104281 RchiOBHm_Chr2g0104311 RchiOBHm_Chr2g0104331 RchiOBHm_Chr2g0104361 RchiOBHm_Chr2g0104381 RchiOBHm_Chr2g0104391 RchiOBHm_Chr2g0104431 RchiOBHm_Chr2g0104441 RchiOBHm_Chr2g0104541 RchiOBHm_Chr2g0104611 RchiOBHm_Chr2g0104741 RchiOBHm_Chr2g0104761 RchiOBHm_Chr2g0158291 RchiOBHm_Chr2g0170371 RchiOBHm_Chr2g0170631 RchiOBHm_Chr3g0473971 RchiOBHm_Chr3g0480491 RchiOBHm_Chr3g0480541 RchiOBHm_Chr3g0480561 RchiOBHm_Chr3g0485281 RchiOBHm_Chr3g0485291 RchiOBHm_Chr4g0411541 RchiOBHm_Chr6g0285471
rosa_laevigata RLG00000008347 RLG00000012637 RLG00000016961 RLG00000017293 RLG00000017305 RLG00000017308 RLG00000017312 RLG00000017314 RLG00000017317 RLG00000017318 RLG00000017319 RLG00000017321 RLG00000017322 RLG00000017323 RLG00000017324 RLG00000017325 RLG00000017327 RLG00000017328 RLG00000017329 RLG00000017330 RLG00000017334 RLG00000020833 RLG00000021073 RLG00000021950 RLG00000021971 RLG00000023154 RLG00000023157 RLG00000023467 RLG00000023468 RLG00000023525 RLG00000023963 RLG00000033265 RLG00000033267 RLG00000033268
rosa_multiflora Rmu_co8053082.1_g000001 Rmu_co8210358.1_g000001 Rmu_co8331661.1_g000001 Rmu_co8477427.1_g000002 Rmu_co8483217.1_g000001 Rmu_sc0000284.1_g000004 Rmu_sc0000676.1_g000017 Rmu_sc0000676.1_g000019 Rmu_sc0000676.1_g000021 Rmu_sc0002205.1_g000038 Rmu_sc0002205.1_g000041 Rmu_sc0002316.1_g000010 Rmu_sc0002316.1_g000012 Rmu_sc0002316.1_g000040 Rmu_sc0002316.1_g000071 Rmu_sc0002316.1_g000072 Rmu_sc0002316.1_g000095 Rmu_sc0002316.1_g000102 Rmu_sc0002414.1_g000018 Rmu_sc0002576.1_g000002 Rmu_sc0002576.1_g000003 Rmu_sc0002687.1_g000010 Rmu_sc0002687.1_g000017 Rmu_sc0003660.1_g000001 Rmu_sc0003787.1_g000014 Rmu_sc0003787.1_g000020 Rmu_sc0003787.1_g000024 Rmu_sc0003787.1_g000029 Rmu_sc0004059.1_g000026 Rmu_sc0005137.1_g000021 Rmu_sc0006550.1_g000001 Rmu_sc0006561.1_g000025 Rmu_sc0006561.1_g000033 Rmu_sc0007704.1_g000005 Rmu_sc0008904.1_g000005 Rmu_sc0010270.1_g000002 Rmu_sc0010307.1_g000001 Rmu_sc0011601.1_g000013 Rmu_sc0012694.1_g000005 Rmu_sc0013101.1_g000008 Rmu_sc0019029.1_g000001 Rmu_sc0019728.1_g000001 Rmu_sc0025829.1_g000001 Rmu_sc0025829.1_g000002 Rmu_ssc0000257.1_g000007 Rmu_ssc0000257.1_g000008
rosa_roxburghii Rroxscaffold_1G00021710 Rroxscaffold_1G00049350 Rroxscaffold_1G00049360 Rroxscaffold_2G00081160 Rroxscaffold_2G00081360 Rroxscaffold_2G00081390 Rroxscaffold_2G00091540 Rroxscaffold_2G00138790 Rroxscaffold_2G00138800 Rroxscaffold_2G00138920 Rroxscaffold_2G00139000 Rroxscaffold_2G00139010 Rroxscaffold_2G00139020 Rroxscaffold_2G00139030 Rroxscaffold_2G00139040 Rroxscaffold_2G00139050 Rroxscaffold_2G00139060 Rroxscaffold_2G00139070 Rroxscaffold_2G00139090 Rroxscaffold_2G00139100 Rroxscaffold_3G00257470 Rroxscaffold_4G00318040 Rroxscaffold_4G00323740 Rroxscaffold_4G00323750 Rroxscaffold_5G00356140 Rroxscaffold_6G00401280 Rroxscaffold_6G00401290 Rroxscaffold_7G00183120
rosa_rugosa Rorug01G0060500 Rorug01G0060900 Rorug02G0119500 Rorug02G0119600 Rorug02G0119700 Rorug02G0119700 Rorug02G0119700 Rorug02G0119800.1 Rorug02G0119900.1 Rorug02G0120000 Rorug02G0120100 Rorug02G0120200 Rorug02G0120300 Rorug02G0120400 Rorug02G0120500 Rorug02G0120700 Rorug02G0120700 Rorug02G0122300 Rorug02G0122300 Rorug02G0122400.1 Rorug02G0466700 Rorug02G0466700 Rorug02G0466800 Rorug02G0548600 Rorug02G0549900 Rorug02G0550000 Rorug03G0184200 Rorug04G0107900 Rorug05G0121500 Rorug06G0174800
rosa_samantha Rh1AG198400 Rh1CG074700 Rh1CG075000 Rh1CG183700 Rh2AG169700 Rh2AG169800 Rh2AG169900 Rh2AG170000 Rh2AG170300 Rh2AG170400 Rh2AG171000 Rh2AG171100 Rh2AG171600 Rh2AG172400 Rh2AG172500 Rh2AG532900 Rh2AG620000 Rh2AG622500 Rh2AG622800 Rh2AG623000 Rh2BG176200 Rh2BG176300 Rh2BG176400 Rh2BG176500 Rh2BG176600 Rh2BG176900 Rh2BG177000 Rh2BG177100 Rh2BG177200 Rh2BG177300 Rh2BG177600 Rh2BG177700 Rh2BG177900 Rh2BG178000 Rh2BG178200 Rh2BG178300 Rh2BG178500 Rh2BG179000 Rh2BG180000 Rh2BG180100 Rh2BG259200 Rh2BG546700 Rh2BG630800 Rh2BG633500 Rh2CG176500 Rh2CG177500 Rh2CG177700 Rh2DG174400 Rh2DG174600 Rh2DG174900 Rh2DG175100 Rh2DG175200 Rh2DG175300 Rh2DG175600 Rh2DG175700 Rh2DG175800 Rh2DG175900 Rh2DG176000 Rh2DG176300 Rh2DG176400 Rh2DG176500 Rh2DG176600 Rh2DG176900 Rh2DG177100 Rh2DG177200 Rh2DG177800 Rh2DG177900 Rh2DG178700 Rh2DG178800 Rh2DG555700 Rh2DG643700 Rh2DG646300 Rh3AG235600 Rh3AG235900 Rh3AG236200 Rh3AG266700 Rh3AG266900 Rh3BG216200 Rh3BG269100 Rh3CG212700 Rh3CG265800 Rh4AG168900 Rh4BG165500 Rh4CG180300 Rh5BG261500 Rh6AG286300 Rh6BG287500 Rh6CG288600 Rh7AG022600
rosa_wichuraiana Rw0G008240 Rw0G008250 Rw0G013190 Rw0G013200 Rw2G013230 Rw2G013240 Rw2G013270 Rw2G013280 Rw2G013290 Rw2G013300 Rw2G013330 Rw2G013340 Rw2G013350 Rw2G013360 Rw2G013370 Rw2G013380 Rw2G013410 Rw2G013570 Rw2G044120 Rw2G051370 Rw2G051590 Rw3G017160 Rw3G021150 Rw3G021160 Rw3G023730 Rw3G023740 Rw4G014030 Rw6G024590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1535
Acc36I ACCTGC 1 cut(s) 1783
AccB1I GGYRCC 2 cut(s) 99, 481
AccB7I CCANNNNNTGG 1 cut(s) 1993
AccBSI CCGCTC 1 cut(s) 1427
AccII CGCG 1 cut(s) 1110
AccIII TCCGGA 1 cut(s) 1276
AciI CCGC 5 cut(s) 103, 1110, 1302, 1380, 1427
AclWI GGATC 5 cut(s) 639, 856, 976, 1480, 2316
AcoI YGGCCR 5 cut(s) 1080, 1111, 1643, 1921, 1933
AcsI RAATTY 3 cut(s) 1684, 1938, 2180
AcuI CTGAAG 2 cut(s) 236, 507
AfaI GTAC 4 cut(s) 37, 361, 711, 1659
AfeI AGCGCT 1 cut(s) 1061
AfiI CCNNNNNNNGG 3 cut(s) 698, 935, 1993
AflII CTTAAG 2 cut(s) 1820, 2033
AflIII ACRYGT 1 cut(s) 467
AjnI CCWGG 2 cut(s) 828, 2149
AjuI GAANNNNNNNTTGG 6 cut(s) 593, 625, 680, 712, 922, 954
Alw21I GWGCWC 5 cut(s) 31, 239, 414, 1022, 1377
Alw26I GTCTC 6 cut(s) 9, 105, 371, 685, 2272, 2339
Alw44I GTGCAC 1 cut(s) 235
AlwI GGATC 5 cut(s) 639, 856, 976, 1480, 2316
Ama87I CYCGRG 3 cut(s) 417, 1661, 2222
Aor13HI TCCGGA 1 cut(s) 1276
Aor51HI AGCGCT 1 cut(s) 1061
AoxI GGCC 7 cut(s) 700, 1080, 1111, 1504, 1643, 1921, 1933
ApaLI GTGCAC 1 cut(s) 235
ApeKI GCWGC 7 cut(s) 76, 261, 584, 742, 770, 2244, 2290
ApoI RAATTY 3 cut(s) 1684, 1938, 2180
Asp700I GAANNNNTTC 2 cut(s) 665, 1347
AspA2I CCTAGG 1 cut(s) 476
AspLEI GCGC 3 cut(s) 76, 493, 1062
AspS9I GGNCC 5 cut(s) 700, 735, 1282, 1505, 1867
AsuHPI GGTGA 5 cut(s) 1369, 1447, 2021, 2041, 2413
AsuII TTCGAA 1 cut(s) 328
AvaI CYCGRG 3 cut(s) 417, 1661, 2222
AvaII GGWCC 3 cut(s) 735, 1282, 1867
AvrII CCTAGG 1 cut(s) 476
BaeGI GKGCMC 1 cut(s) 239
BaeI ACNNNNGTAYC 2 cut(s) 1565, 1598
BalI TGGCCA 2 cut(s) 1082, 1645
BanI GGYRCC 2 cut(s) 99, 481
BanII GRGCYC 3 cut(s) 858, 1022, 1377
BauI CACGAG 1 cut(s) 1370
BbsI GAAGAC 1 cut(s) 242
Bbv12I GWGCWC 5 cut(s) 31, 239, 414, 1022, 1377
BbvI GCAGC 7 cut(s) 88, 273, 571, 729, 782, 2256, 2277
BccI CCATC 6 cut(s) 199, 502, 1414, 1424, 1497, 1616
BceAI ACGGC 1 cut(s) 505
BcgI CGANNNNNNTGC 2 cut(s) 728, 762
BciT130I CCWGG 2 cut(s) 830, 2151
BclI TGATCA 2 cut(s) 874, 2403
BcoDI GTCTC 6 cut(s) 9, 105, 371, 685, 2272, 2339
BfaI CTAG 9 cut(s) 8, 128, 477, 1265, 1337, 1676, 1784, 1961, 2096
BfmI CTRYAG 4 cut(s) 200, 353, 1119, 2079
BfoI RGCGCY 2 cut(s) 494, 1063
BfrI CTTAAG 2 cut(s) 1820, 2033
BfuAI ACCTGC 1 cut(s) 1783
BglI GCCNNNNNGGC 1 cut(s) 1634
BglII AGATCT 2 cut(s) 1036, 1896
BisI GCNGC 9 cut(s) 77, 262, 585, 743, 771, 1111, 1302, 2245, 2291
BlnI CCTAGG 1 cut(s) 476
BlpI GCTNAGC 1 cut(s) 2042
BlsI GCNGC 9 cut(s) 78, 263, 586, 744, 772, 1112, 1303, 2246, 2292
Bme1390I CCNGG 2 cut(s) 830, 2151
Bme18I GGWCC 3 cut(s) 735, 1282, 1867
BmeT110I CYCGRG 3 cut(s) 417, 1661, 2222
BmgT120I GGNCC 5 cut(s) 700, 735, 1282, 1505, 1867
BmiI GGNNCC 6 cut(s) 101, 483, 736, 1627, 2165, 2364
BmrFI CCNGG 2 cut(s) 830, 2151
BpiI GAAGAC 1 cut(s) 242
BpmI CTGGAG 1 cut(s) 609
Bpu10I CCTNAGC 2 cut(s) 1205, 2120
Bpu1102I GCTNAGC 1 cut(s) 2042
Bpu14I TTCGAA 1 cut(s) 328
BpuEI CTTGAG 3 cut(s) 533, 779, 884
BsaJI CCNNGG 7 cut(s) 476, 609, 1005, 1221, 1662, 1924, 2223
BsaWI WCCGGW 2 cut(s) 1276, 2195
Bsc4I CCNNNNNNNGG 3 cut(s) 698, 935, 1993
Bse1I ACTGG 3 cut(s) 318, 1083, 2269
Bse3DI GCAATG 4 cut(s) 984, 1083, 1356, 2244
BseAI TCCGGA 1 cut(s) 1276
BseBI CCWGG 2 cut(s) 830, 2151
BseDI CCNNGG 7 cut(s) 476, 609, 1005, 1221, 1662, 1924, 2223
BseGI GGATG 8 cut(s) 373, 520, 559, 838, 1001, 1076, 1435, 2444
BseLI CCNNNNNNNGG 3 cut(s) 698, 935, 1993
BseMI GCAATG 4 cut(s) 984, 1083, 1356, 2244
BseMII CTCAG 2 cut(s) 46, 1704
BseNI ACTGG 3 cut(s) 318, 1083, 2269
BseRI GAGGAG 3 cut(s) 224, 536, 2357
BseSI GKGCMC 1 cut(s) 239
BseXI GCAGC 7 cut(s) 88, 273, 571, 729, 782, 2256, 2277
BseYI CCCAGC 1 cut(s) 1458
BsgI GTGCAG 2 cut(s) 280, 2151
Bsh1236I CGCG 1 cut(s) 1110
BshFI GGCC 7 cut(s) 702, 1082, 1113, 1506, 1645, 1923, 1935
BshNI GGYRCC 2 cut(s) 99, 481
BsiHKAI GWGCWC 5 cut(s) 31, 239, 414, 1022, 1377
BsiHKCI CYCGRG 3 cut(s) 417, 1661, 2222
BsiSI CCGG 4 cut(s) 80, 97, 1277, 2196
BslFI GGGAC 2 cut(s) 748, 1562
BslI CCNNNNNNNGG 3 cut(s) 698, 935, 1993
BsmAI GTCTC 6 cut(s) 9, 105, 371, 685, 2272, 2339
BsmBI CGTCTC 2 cut(s) 371, 2339
BsmFI GGGAC 2 cut(s) 748, 1562
BsnI GGCC 7 cut(s) 702, 1082, 1113, 1506, 1645, 1923, 1935
BsoBI CYCGRG 3 cut(s) 417, 1661, 2222
Bsp119I TTCGAA 1 cut(s) 328
Bsp1286I GDGCHC 6 cut(s) 31, 239, 414, 858, 1022, 1377
Bsp13I TCCGGA 1 cut(s) 1276
Bsp1407I TGTACA 1 cut(s) 709
Bsp1720I GCTNAGC 1 cut(s) 2042
BspACI CCGC 5 cut(s) 103, 1110, 1302, 1380, 1427
BspANI GGCC 7 cut(s) 702, 1082, 1113, 1506, 1645, 1923, 1935
BspCNI CTCAG 2 cut(s) 45, 1705
BspEI TCCGGA 1 cut(s) 1276
BspFNI CGCG 1 cut(s) 1110
BspLI GGNNCC 6 cut(s) 101, 483, 736, 1627, 2165, 2364
BspMAI CTGCAG 2 cut(s) 357, 2083
BspMI ACCTGC 1 cut(s) 1783
BspPI GGATC 5 cut(s) 639, 856, 976, 1480, 2316
BspT104I TTCGAA 1 cut(s) 328
BspT107I GGYRCC 2 cut(s) 99, 481
BspTI CTTAAG 2 cut(s) 1820, 2033
BsrBI CCGCTC 1 cut(s) 1427
BsrDI GCAATG 4 cut(s) 984, 1083, 1356, 2244
BsrGI TGTACA 1 cut(s) 709
BsrI ACTGG 3 cut(s) 318, 1083, 2269
BssECI CCNNGG 7 cut(s) 476, 609, 1005, 1221, 1662, 1924, 2223
BssSI CACGAG 1 cut(s) 1370
BssT1I CCWWGG 5 cut(s) 476, 609, 1005, 1221, 1924
Bst2BI CACGAG 1 cut(s) 1370
Bst2UI CCWGG 2 cut(s) 830, 2151
Bst4CI ACNGT 6 cut(s) 779, 1766, 1878, 2057, 2319, 2376
Bst6I CTCTTC 4 cut(s) 325, 330, 420, 1338
BstAFI CTTAAG 2 cut(s) 1820, 2033
BstAPI GCANNNNNTGC 1 cut(s) 1468
BstAUI TGTACA 1 cut(s) 709
BstBI TTCGAA 1 cut(s) 328
BstC8I GCNNGC 7 cut(s) 747, 1115, 1194, 1198, 1889, 2079, 2295
BstDEI CTNAG 6 cut(s) 32, 1205, 1472, 1713, 2042, 2120
BstF5I GGATG 8 cut(s) 373, 520, 559, 838, 1001, 1076, 1435, 2444
BstFNI CGCG 1 cut(s) 1110
BstH2I RGCGCY 2 cut(s) 494, 1063
BstHHI GCGC 3 cut(s) 76, 493, 1062
BstMAI GTCTC 6 cut(s) 9, 105, 371, 685, 2272, 2339
BstNI CCWGG 2 cut(s) 830, 2151
BstNSI RCATGY 3 cut(s) 471, 1471, 1654
BstSCI CCNGG 2 cut(s) 828, 2149
BstSFI CTRYAG 4 cut(s) 200, 353, 1119, 2079
BstSLI GKGCMC 1 cut(s) 239
BstUI CGCG 1 cut(s) 1110
BstV1I GCAGC 7 cut(s) 88, 273, 571, 729, 782, 2256, 2277
BstV2I GAAGAC 1 cut(s) 242
BstX2I RGATCY 3 cut(s) 1036, 1896, 2308
BstXI CCANNNNNNTGG 1 cut(s) 1090
BstYI RGATCY 3 cut(s) 1036, 1896, 2308
BsuRI GGCC 7 cut(s) 702, 1082, 1113, 1506, 1645, 1923, 1935
BtsCI GGATG 8 cut(s) 373, 520, 559, 838, 1001, 1076, 1435, 2444
BtsI GCAGTG 1 cut(s) 1949
BtsIMutI CAGTG 4 cut(s) 1076, 1440, 1949, 2276
BveI ACCTGC 1 cut(s) 1783
Cac8I GCNNGC 7 cut(s) 747, 1115, 1194, 1198, 1889, 2079, 2295
CfoI GCGC 3 cut(s) 76, 493, 1062
Cfr13I GGNCC 5 cut(s) 700, 735, 1282, 1505, 1867
Csp6I GTAC 4 cut(s) 36, 360, 710, 1658
CspCI CAANNNNNGTGG 2 cut(s) 1944, 1979
CviQI GTAC 4 cut(s) 36, 360, 710, 1658
DdeI CTNAG 6 cut(s) 32, 1205, 1472, 1713, 2042, 2120
EaeI YGGCCR 5 cut(s) 1080, 1111, 1643, 1921, 1933
Eam1104I CTCTTC 4 cut(s) 325, 330, 420, 1338
EarI CTCTTC 4 cut(s) 325, 330, 420, 1338
Ecl136II GAGCTC 2 cut(s) 1020, 1375
Eco130I CCWWGG 5 cut(s) 476, 609, 1005, 1221, 1924
Eco24I GRGCYC 3 cut(s) 858, 1022, 1377
Eco32I GATATC 2 cut(s) 1918, 2416
Eco47I GGWCC 3 cut(s) 735, 1282, 1867
Eco47III AGCGCT 1 cut(s) 1061
Eco53kI GAGCTC 2 cut(s) 1020, 1375
Eco57I CTGAAG 2 cut(s) 236, 507
Eco88I CYCGRG 3 cut(s) 417, 1661, 2222
EcoICRI GAGCTC 2 cut(s) 1020, 1375
EcoO109I RGGNCCY 1 cut(s) 1867
EcoRII CCWGG 2 cut(s) 828, 2149
EcoRV GATATC 2 cut(s) 1918, 2416
EcoT14I CCWWGG 5 cut(s) 476, 609, 1005, 1221, 1924
EcoT38I GRGCYC 3 cut(s) 858, 1022, 1377
ErhI CCWWGG 5 cut(s) 476, 609, 1005, 1221, 1924
Esp3I CGTCTC 2 cut(s) 371, 2339
FalI AAGNNNNNCTT 4 cut(s) 650, 682, 680, 712
FaqI GGGAC 2 cut(s) 748, 1562
FauNDI CATATG 1 cut(s) 1541
FbaI TGATCA 2 cut(s) 874, 2403
Fnu4HI GCNGC 9 cut(s) 77, 262, 585, 743, 771, 1111, 1302, 2245, 2291
FokI GGATG 8 cut(s) 380, 527, 566, 845, 988, 1083, 1442, 2431
FriOI GRGCYC 3 cut(s) 858, 1022, 1377
Fsp4HI GCNGC 9 cut(s) 77, 262, 585, 743, 771, 1111, 1302, 2245, 2291
FspBI CTAG 9 cut(s) 8, 128, 477, 1265, 1337, 1676, 1784, 1961, 2096
GlaI GCGC 3 cut(s) 75, 492, 1061
GluI GCNGC 9 cut(s) 77, 262, 585, 743, 771, 1111, 1302, 2245, 2291
GsaI CCCAGC 1 cut(s) 1462
GsuI CTGGAG 1 cut(s) 609
HaeII RGCGCY 2 cut(s) 494, 1063
HaeIII GGCC 7 cut(s) 702, 1082, 1113, 1506, 1645, 1923, 1935
HapII CCGG 4 cut(s) 80, 97, 1277, 2196
HhaI GCGC 3 cut(s) 76, 493, 1062
Hin6I GCGC 3 cut(s) 74, 491, 1060
HinP1I GCGC 3 cut(s) 74, 491, 1060
HincII GTYRAC 2 cut(s) 311, 1569
HindII GTYRAC 2 cut(s) 311, 1569
HindIII AAGCTT 4 cut(s) 302, 1885, 2039, 2438
HinfI GANTC 6 cut(s) 131, 800, 933, 1175, 1348, 1498
HpaII CCGG 4 cut(s) 80, 97, 1277, 2196
HphI GGTGA 5 cut(s) 1369, 1447, 2021, 2041, 2413
Hpy166II GTNNAC 5 cut(s) 229, 237, 311, 1569, 2029
Hpy188I TCNGA 9 cut(s) 20, 110, 169, 255, 853, 1180, 1353, 1683, 2090
Hpy8I GTNNAC 5 cut(s) 229, 237, 311, 1569, 2029
Hpy99I CGWCG 1 cut(s) 2342
HpyAV CCTTC 6 cut(s) 400, 520, 724, 869, 1639, 2377
HpyCH4III ACNGT 6 cut(s) 779, 1766, 1878, 2057, 2319, 2376
HpyCH4IV ACGT 4 cut(s) 380, 1840, 2013, 2262
HpyF3I CTNAG 6 cut(s) 32, 1205, 1472, 1713, 2042, 2120
HpySE526I ACGT 4 cut(s) 380, 1840, 2013, 2262
HspAI GCGC 3 cut(s) 74, 491, 1060
Kpn2I TCCGGA 1 cut(s) 1276
Ksp22I TGATCA 2 cut(s) 874, 2403
LmnI GCTCC 4 cut(s) 99, 549, 1424, 1625
Lsp1109I GCAGC 7 cut(s) 88, 273, 571, 729, 782, 2256, 2277
MaeI CTAG 9 cut(s) 8, 128, 477, 1265, 1337, 1676, 1784, 1961, 2096
MaeII ACGT 4 cut(s) 380, 1840, 2013, 2262
MaeIII GTNAC 6 cut(s) 463, 907, 1405, 1453, 1770, 2018
MbiI CCGCTC 1 cut(s) 1427
MfeI CAATTG 1 cut(s) 878
MflI RGATCY 3 cut(s) 1036, 1896, 2308
MhlI GDGCHC 6 cut(s) 31, 239, 414, 858, 1022, 1377
MlsI TGGCCA 2 cut(s) 1082, 1645
MluNI TGGCCA 2 cut(s) 1082, 1645
MlyI GAGTC 2 cut(s) 125, 1507
MmeI TCCRAC 1 cut(s) 492
Mox20I TGGCCA 2 cut(s) 1082, 1645
MroI TCCGGA 1 cut(s) 1276
MroXI GAANNNNTTC 2 cut(s) 665, 1347
MscI TGGCCA 2 cut(s) 1082, 1645
MseI TTAA 5 cut(s) 348, 1821, 1872, 2034, 2169
MslI CAYNNNNRTG 2 cut(s) 1313, 2399
Msp20I TGGCCA 2 cut(s) 1082, 1645
MspCI CTTAAG 2 cut(s) 1820, 2033
MspI CCGG 4 cut(s) 80, 97, 1277, 2196
MspR9I CCNGG 2 cut(s) 830, 2151
MunI CAATTG 1 cut(s) 878
MvaI CCWGG 2 cut(s) 830, 2151
MvnI CGCG 1 cut(s) 1110
NdeI CATATG 1 cut(s) 1541
NlaIV GGNNCC 6 cut(s) 101, 483, 736, 1627, 2165, 2364
NmeAIII GCCGAG 2 cut(s) 977, 1613
NmuCI GTSAC 4 cut(s) 907, 1405, 1453, 2018
NspI RCATGY 3 cut(s) 471, 1471, 1654
NspV TTCGAA 1 cut(s) 328
PaeR7I CTCGAG 1 cut(s) 417
PciI ACATGT 1 cut(s) 467
PcsI WCGNNNNNNNCGW 1 cut(s) 368
PdmI GAANNNNTTC 2 cut(s) 665, 1347
PfeI GAWTC 4 cut(s) 800, 933, 1175, 1348
PflMI CCANNNNNTGG 1 cut(s) 1993
PfoI TCCNGGA 1 cut(s) 828
PkrI GCNGC 9 cut(s) 78, 263, 586, 744, 772, 1112, 1303, 2246, 2292
PleI GAGTC 2 cut(s) 125, 1506
PpsI GAGTC 2 cut(s) 125, 1506
PpuMI RGGWCCY 1 cut(s) 1867
PscI ACATGT 1 cut(s) 467
PsiI TTATAA 1 cut(s) 1535
Psp124BI GAGCTC 2 cut(s) 1022, 1377
Psp5II RGGWCCY 1 cut(s) 1867
Psp6I CCWGG 2 cut(s) 828, 2149
PspFI CCCAGC 1 cut(s) 1458
PspGI CCWGG 2 cut(s) 828, 2149
PspN4I GGNNCC 6 cut(s) 101, 483, 736, 1627, 2165, 2364
PspPI GGNCC 5 cut(s) 700, 735, 1282, 1505, 1867
PspPPI RGGWCCY 1 cut(s) 1867
PsrI GAACNNNNNNTAC 2 cut(s) 2355, 2387
PstI CTGCAG 2 cut(s) 357, 2083
PsuI RGATCY 3 cut(s) 1036, 1896, 2308
RsaI GTAC 4 cut(s) 37, 361, 711, 1659
RsaNI GTAC 4 cut(s) 36, 360, 710, 1658
RseI CAYNNNNRTG 2 cut(s) 1313, 2399
SacI GAGCTC 2 cut(s) 1022, 1377
SaqAI TTAA 5 cut(s) 348, 1821, 1872, 2034, 2169
SatI GCNGC 9 cut(s) 77, 262, 585, 743, 771, 1111, 1302, 2245, 2291
Sau96I GGNCC 5 cut(s) 700, 735, 1282, 1505, 1867
SchI GAGTC 2 cut(s) 125, 1507
ScrFI CCNGG 2 cut(s) 830, 2151
SduI GDGCHC 6 cut(s) 31, 239, 414, 858, 1022, 1377
SfcI CTRYAG 4 cut(s) 200, 353, 1119, 2079
Sfr274I CTCGAG 1 cut(s) 417
SfuI TTCGAA 1 cut(s) 328
SinI GGWCC 3 cut(s) 735, 1282, 1867
SlaI CTCGAG 1 cut(s) 417
SmiMI CAYNNNNRTG 2 cut(s) 1313, 2399
SmlI CTYRAG 6 cut(s) 417, 548, 794, 863, 1820, 2033
SmoI CTYRAG 6 cut(s) 417, 548, 794, 863, 1820, 2033
SsiI CCGC 5 cut(s) 103, 1110, 1302, 1380, 1427
SspMI CTAG 9 cut(s) 8, 128, 477, 1265, 1337, 1676, 1784, 1961, 2096
SstI GAGCTC 2 cut(s) 1022, 1377
StyD4I CCNGG 2 cut(s) 828, 2149
StyI CCWWGG 5 cut(s) 476, 609, 1005, 1221, 1924
TaaI ACNGT 6 cut(s) 779, 1766, 1878, 2057, 2319, 2376
TaiI ACGT 4 cut(s) 383, 1843, 2016, 2265
TaqII GACCGA 1 cut(s) 752
TatI WGTACW 1 cut(s) 709
TauI GCSGC 2 cut(s) 1113, 1304
TfiI GAWTC 4 cut(s) 800, 933, 1175, 1348
Tru1I TTAA 5 cut(s) 348, 1821, 1872, 2034, 2169
Tru9I TTAA 5 cut(s) 348, 1821, 1872, 2034, 2169
TscAI CASTG 4 cut(s) 1083, 1447, 1956, 2276
TseFI GTSAC 4 cut(s) 907, 1405, 1453, 2018
TseI GCWGC 7 cut(s) 76, 261, 584, 742, 770, 2244, 2290
Tsp45I GTSAC 4 cut(s) 907, 1405, 1453, 2018
TspDTI ATGAA 8 cut(s) 455, 554, 570, 729, 984, 1152, 1356, 1897
TspRI CASTG 4 cut(s) 1083, 1447, 1956, 2276
Van91I CCANNNNNTGG 1 cut(s) 1993
Vha464I CTTAAG 2 cut(s) 1820, 2033
VneI GTGCAC 1 cut(s) 235
VpaK11BI GGWCC 3 cut(s) 735, 1282, 1867
XapI RAATTY 3 cut(s) 1684, 1938, 2180
XbaI TCTAGA 1 cut(s) 1336
XceI RCATGY 3 cut(s) 471, 1471, 1654
XhoI CTCGAG 1 cut(s) 417
XmaJI CCTAGG 1 cut(s) 476
XmnI GAANNNNTTC 2 cut(s) 665, 1347
XspI CTAG 9 cut(s) 8, 128, 477, 1265, 1337, 1676, 1784, 1961, 2096
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.