Rw3G021160

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0000039
Physical Location & Seq
Reverse (-)
25392466 .. 25395861
3396 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G021160.1

Sequence Viewer

Length: 2442 bp
ATGTGTTGTGTAATGGCAGTGGCATTGATAGGAGGAGCGGGTTTGTCAGTAACATTTTGGACGCTGTGTGATTCTGTTAAGCAAGTGAAGGAGCAGTTCAAACCCCAACTCGGGGATATCAAATCCACTCTGGACTCTTTGGAACCGCTGATCAAAGAGATGGCAGAGTGTGAGAAGGAATTGGGTGGTCAAGGGGAGGAACTAGAGAATCTTAGAGTGGAAATGGGGGAAGGAGTGGAGCTTATTCGCAAGTGCTCGAAAGCGAGCAGGCTCAAGCAGTATAAGTATGGCAGCCGGCTTGTTAAATTGAGCAAGTCACTTCAAAGATTGTTGGGGATACTCAAAGTGCAGGGAATAAGGGATGTGAAGAAGGCATTGGTTTCAGTGAGGAACATTGAGAATGTCGTGCAGAGAATCGAAGGGAATTGTGTGATACAGAACAGTCAGTCTGAAAAAATTCAAGGGTGGTGTGCTGTGCCTGAGCCTCCAACAATTACGGTTGGATTGGATGTGCCTTTGTGGGAATTAAAGAAGAAGCTCTTTACTGACGGAGTTTCGATGGTGATAATTACTGCTCCTGGAGGATGTGGAAAAACTACACTGGCAACAAAGTTGTGCCAAGATGCAGAGGTCAAAGATAAATTCAAGAGCAATGTCTTCTTTGTTACTGTTTCAAAGAAGCCCAACTTATACCTAATTGTACAAGAGCTGTATCATGGAACGGGTGGCCAGGTCCCCGATTTCCAAAATGAAGCAACTGCAGTTAGCTGGCTGCAGCAATTTCTGAAGCAAACAGGACAAAATCCTATGTTGTTGGTCCTAGATGATGTCTGGGCAGGATCGGAATTCATTCTTGAGAAGTTTGATGAATTAAAGATGTCAGATTGCAAGATATTGGTAACATCATGGTCTGCTTTTCCAAGATTTGGTTCTCCATATTATCTAGAATGTTTGAACGATGAAGATGCAATGGCACTTTTTCACCATGCAGCATCCTTAGGAGACAGGAGCTCTTATATTTCAGAAGATCTTTCAAGAAAGATAGTAGAACGTTGTATGGGATTTCCACTTGCAATTACAGTTGTTGGAAGATCACTATGCGGGCAGCCAATAGAGATTTGGCAAAAAAGAGTATTGGAATGGTCTAGAGGTTCTTCTGTTCTTGAGTCTGAACATGATTTGCTTGATTACCTCCAGAGTAGCTTAGATGCTTTGGATGGAGAGAAGTCTCTTATCAGGGAATGCTTCATAGACCTTGGTTTATTTCCCAAAGATCAAAGAATCTCTGCTACTGTCCTCATCGATATGTGGGCAGAGTTATATGGCATAGAAGACATTTCGTCCATTGTGAATCTCTATGAACTCGCCTCTCGGAGTTTGGCTAATCTGGTAGACACAAGGAACGACAACAAGGATGGCGATGGCTATTACACTGAACACTTTGTTACACAGCTTGACATGCTTAGGGAGTTGGCTATCCAACAGATTAGTCGGGAACCACTACAACAGAGAATGAGACTTATGATTGACATAAGTGGAGACAACTTTCCTATGTGGTGGAAAGAACAAAAGTATCAATACCTCATCACTCGCGTTGTATCTATCTCGACAGACGAAGAGTTTTCATCTAAATGGCAAAACTTGCAACTACCAGAAGCAGAGGTTTTGATTTTGGACTTTCAGACCAAGTATTATGCCTTACCTGGGTTTGTGGAGAAAATGAATAAACTGAAGACTCTAGCACTCACAAGTTCGGGTATCAGTCCTGCTGAGTTGAATAACTTTCAACTAGTTGATTCGAAACCAAATTTAAAGAGAATCAGATTAGAACGAATTTCATTTCCTTCGATAAGCAGGTATCCTATACAGTTGAAAAGTTTGAAAAAGATTTCTCTATTCATGTGTAGCATCGGCCAAGGTTTTGGTAACGGTTACATACATATATCAGAGGCGTTTCCAAATCTTGTTGAAATGAATATTGATTACTGCAATGATTTGGTGGAGCTGCCTGCCGATCTCTGTGACCTTATTCAATTGAAGAAACTCAGTGTCACCAATTCTCATAAGCTCTCCGTCTTACCTGAAGACATTGGAAATCTAGTAAATTTAGAGATGCTGAGGCTTAGGTGTTGTACAGACTTGTCAGAGTTACCCGGCTCCATTAGGAACCTCAAGAACTTAAACTTGCTTGACATATTGAATTGCTTTAGCCTTAAGGAATTGCCTGAAGACATTGGAGAAATGTGCAGTTTAGAGAAGCTCAACATGAGACAGTGCTCGAGATTGAATGAGTTGCCTTCATCAGTTGTGGATCTTGAAAAGCTAATGGAAGTGACATGTGATGAAGAGACAGAAATCTTATGGGAGCCCTTCTTACCGTATCTCAGGAACATACACATAAAGGTTGTGAAAGAAGATATGAACCTGAATTGGCTCCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

813

Amino Acids

92.25

Weight (kDa)

5.19

Isoelectric Point (pI)

42.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 9 - 132 2.6e-17 Arabidopsis broad-spectrum mildew resistance protein RPW8
NB-ARC PF00931 175 - 329 5.8e-15 NB-ARC domain
AAA_22 PF13401 186 - 285 4.8e-07 AAA domain
LRR_14 PF23598 652 - 732 1.1e-08 Leucine-rich repeat region
LRR_14 PF23598 735 - 786 2.4e-07 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000039)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66890 AT5G66900 AT5G66900 AT5G66900 AT5G66910
fragaria_vesca FvH4_1g15090 FvH4_1g15120 FvH4_1g15130 FvH4_1g15140 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15170 FvH4_1g15171 FvH4_1g15171 FvH4_1g15200 FvH4_1g15200 FvH4_1g15200 FvH4_1g15210 FvH4_1g15210 FvH4_1g15210 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15230 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15500 FvH4_1g15500 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_2g19760 FvH4_3g12820 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_6g18970 FvH4_6g42370 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g50100 FvH4_6g50100 FvH4_6g50110 FvH4_6g50130 FvH4_6g50140 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50330
malus_domestica MD00G1053700.v1.1 MD02G1164300.v1.1 MD02G1164400.v1.1 MD02G1164700.v1.1 MD02G1164800.v1.1 MD02G1165300.v1.1 MD02G1165400.v1.1 MD02G1165500.v1.1 MD02G1165600.v1.1 MD02G1165900.v1.1 MD02G1166000.v1.1 MD02G1166100.v1.1 MD02G1166300.v1.1 MD08G1084900.v1.1 MD15G1263100.v1.1 MD15G1263300.v1.1 MD15G1276900.v1.1 MD15G1277200.v1.1 MD15G1277400.v1.1 MD15G1277700.v1.1 MD15G1277800.v1.1 MD15G1278000.v1.1 MD15G1278100.v1.1 MD15G1278400.v1.1 MD15G1278600.v1.1
prunus_persica Prupe.7G138500_v2.0.a1 Prupe.7G138500_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138700_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138900_v2.0.a1 Prupe.7G139100_v2.0.a1 Prupe.7G139200_v2.0.a1 Prupe.7G139500_v2.0.a1 Prupe.7G139600_v2.0.a1 Prupe.7G139700_v2.0.a1 Prupe.7G139800_v2.0.a1
pyrus_communis pycom02g13150 pycom02g13160 pycom02g13190 pycom02g13200 pycom02g13220 pycom02g13230 pycom02g13250 pycom02g13260 pycom02g13270 pycom02g13300 pycom02g13310 pycom02g13330 pycom15g23980 pycom15g23990 pycom15g24030 pycom15g24040 pycom15g24050 pycom15g24070 pycom15g24090 pycom15g24160
rosa_chinensis RchiOBHm_Chr1g0326431 RchiOBHm_Chr1g0326451 RchiOBHm_Chr2g0104121 RchiOBHm_Chr2g0104151 RchiOBHm_Chr2g0104171 RchiOBHm_Chr2g0104181 RchiOBHm_Chr2g0104191 RchiOBHm_Chr2g0104201 RchiOBHm_Chr2g0104211 RchiOBHm_Chr2g0104241 RchiOBHm_Chr2g0104261 RchiOBHm_Chr2g0104281 RchiOBHm_Chr2g0104311 RchiOBHm_Chr2g0104331 RchiOBHm_Chr2g0104361 RchiOBHm_Chr2g0104381 RchiOBHm_Chr2g0104391 RchiOBHm_Chr2g0104431 RchiOBHm_Chr2g0104441 RchiOBHm_Chr2g0104541 RchiOBHm_Chr2g0104611 RchiOBHm_Chr2g0104741 RchiOBHm_Chr2g0104761 RchiOBHm_Chr2g0158291 RchiOBHm_Chr2g0170371 RchiOBHm_Chr2g0170631 RchiOBHm_Chr3g0473971 RchiOBHm_Chr3g0480491 RchiOBHm_Chr3g0480541 RchiOBHm_Chr3g0480561 RchiOBHm_Chr3g0485281 RchiOBHm_Chr3g0485291 RchiOBHm_Chr4g0411541 RchiOBHm_Chr6g0285471
rosa_laevigata RLG00000008347 RLG00000012637 RLG00000016961 RLG00000017293 RLG00000017305 RLG00000017308 RLG00000017312 RLG00000017314 RLG00000017317 RLG00000017318 RLG00000017319 RLG00000017321 RLG00000017322 RLG00000017323 RLG00000017324 RLG00000017325 RLG00000017327 RLG00000017328 RLG00000017329 RLG00000017330 RLG00000017334 RLG00000020833 RLG00000021073 RLG00000021950 RLG00000021971 RLG00000023154 RLG00000023157 RLG00000023467 RLG00000023468 RLG00000023525 RLG00000023963 RLG00000033265 RLG00000033267 RLG00000033268
rosa_multiflora Rmu_co8053082.1_g000001 Rmu_co8210358.1_g000001 Rmu_co8331661.1_g000001 Rmu_co8477427.1_g000002 Rmu_co8483217.1_g000001 Rmu_sc0000284.1_g000004 Rmu_sc0000676.1_g000017 Rmu_sc0000676.1_g000019 Rmu_sc0000676.1_g000021 Rmu_sc0002205.1_g000038 Rmu_sc0002205.1_g000041 Rmu_sc0002316.1_g000010 Rmu_sc0002316.1_g000012 Rmu_sc0002316.1_g000040 Rmu_sc0002316.1_g000071 Rmu_sc0002316.1_g000072 Rmu_sc0002316.1_g000095 Rmu_sc0002316.1_g000102 Rmu_sc0002414.1_g000018 Rmu_sc0002576.1_g000002 Rmu_sc0002576.1_g000003 Rmu_sc0002687.1_g000010 Rmu_sc0002687.1_g000017 Rmu_sc0003660.1_g000001 Rmu_sc0003787.1_g000014 Rmu_sc0003787.1_g000020 Rmu_sc0003787.1_g000024 Rmu_sc0003787.1_g000029 Rmu_sc0004059.1_g000026 Rmu_sc0005137.1_g000021 Rmu_sc0006550.1_g000001 Rmu_sc0006561.1_g000025 Rmu_sc0006561.1_g000033 Rmu_sc0007704.1_g000005 Rmu_sc0008904.1_g000005 Rmu_sc0010270.1_g000002 Rmu_sc0010307.1_g000001 Rmu_sc0011601.1_g000013 Rmu_sc0012694.1_g000005 Rmu_sc0013101.1_g000008 Rmu_sc0019029.1_g000001 Rmu_sc0019728.1_g000001 Rmu_sc0025829.1_g000001 Rmu_sc0025829.1_g000002 Rmu_ssc0000257.1_g000007 Rmu_ssc0000257.1_g000008
rosa_roxburghii Rroxscaffold_1G00021710 Rroxscaffold_1G00049350 Rroxscaffold_1G00049360 Rroxscaffold_2G00081160 Rroxscaffold_2G00081360 Rroxscaffold_2G00081390 Rroxscaffold_2G00091540 Rroxscaffold_2G00138790 Rroxscaffold_2G00138800 Rroxscaffold_2G00138920 Rroxscaffold_2G00139000 Rroxscaffold_2G00139010 Rroxscaffold_2G00139020 Rroxscaffold_2G00139030 Rroxscaffold_2G00139040 Rroxscaffold_2G00139050 Rroxscaffold_2G00139060 Rroxscaffold_2G00139070 Rroxscaffold_2G00139090 Rroxscaffold_2G00139100 Rroxscaffold_3G00257470 Rroxscaffold_4G00318040 Rroxscaffold_4G00323740 Rroxscaffold_4G00323750 Rroxscaffold_5G00356140 Rroxscaffold_6G00401280 Rroxscaffold_6G00401290 Rroxscaffold_7G00183120
rosa_rugosa Rorug01G0060500 Rorug01G0060900 Rorug02G0119500 Rorug02G0119600 Rorug02G0119700 Rorug02G0119700 Rorug02G0119700 Rorug02G0119800.1 Rorug02G0119900.1 Rorug02G0120000 Rorug02G0120100 Rorug02G0120200 Rorug02G0120300 Rorug02G0120400 Rorug02G0120500 Rorug02G0120700 Rorug02G0120700 Rorug02G0122300 Rorug02G0122300 Rorug02G0122400.1 Rorug02G0466700 Rorug02G0466700 Rorug02G0466800 Rorug02G0548600 Rorug02G0549900 Rorug02G0550000 Rorug03G0184200 Rorug04G0107900 Rorug05G0121500 Rorug06G0174800
rosa_samantha Rh1AG198400 Rh1CG074700 Rh1CG075000 Rh1CG183700 Rh2AG169700 Rh2AG169800 Rh2AG169900 Rh2AG170000 Rh2AG170300 Rh2AG170400 Rh2AG171000 Rh2AG171100 Rh2AG171600 Rh2AG172400 Rh2AG172500 Rh2AG532900 Rh2AG620000 Rh2AG622500 Rh2AG622800 Rh2AG623000 Rh2BG176200 Rh2BG176300 Rh2BG176400 Rh2BG176500 Rh2BG176600 Rh2BG176900 Rh2BG177000 Rh2BG177100 Rh2BG177200 Rh2BG177300 Rh2BG177600 Rh2BG177700 Rh2BG177900 Rh2BG178000 Rh2BG178200 Rh2BG178300 Rh2BG178500 Rh2BG179000 Rh2BG180000 Rh2BG180100 Rh2BG259200 Rh2BG546700 Rh2BG630800 Rh2BG633500 Rh2CG176500 Rh2CG177500 Rh2CG177700 Rh2DG174400 Rh2DG174600 Rh2DG174900 Rh2DG175100 Rh2DG175200 Rh2DG175300 Rh2DG175600 Rh2DG175700 Rh2DG175800 Rh2DG175900 Rh2DG176000 Rh2DG176300 Rh2DG176400 Rh2DG176500 Rh2DG176600 Rh2DG176900 Rh2DG177100 Rh2DG177200 Rh2DG177800 Rh2DG177900 Rh2DG178700 Rh2DG178800 Rh2DG555700 Rh2DG643700 Rh2DG646300 Rh3AG235600 Rh3AG235900 Rh3AG236200 Rh3AG266700 Rh3AG266900 Rh3BG216200 Rh3BG269100 Rh3CG212700 Rh3CG265800 Rh4AG168900 Rh4BG165500 Rh4CG180300 Rh5BG261500 Rh6AG286300 Rh6BG287500 Rh6CG288600 Rh7AG022600
rosa_wichuraiana Rw0G008240 Rw0G008250 Rw0G013190 Rw0G013200 Rw2G013230 Rw2G013240 Rw2G013270 Rw2G013280 Rw2G013290 Rw2G013300 Rw2G013330 Rw2G013340 Rw2G013350 Rw2G013360 Rw2G013370 Rw2G013380 Rw2G013410 Rw2G013570 Rw2G044120 Rw2G051370 Rw2G051590 Rw3G017160 Rw3G021150 Rw3G021160 Rw3G023730 Rw3G023740 Rw4G014030 Rw6G024590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1845
AccB7I CCANNNNNTGG 1 cut(s) 926
AccBSI CCGCTC 1 cut(s) 38
AccI GTMKAC 1 cut(s) 1392
AccII CGCG 1 cut(s) 1593
AciI CCGC 3 cut(s) 38, 146, 1101
AclI AACGTT 1 cut(s) 1051
AclWI GGATC 2 cut(s) 847, 2319
AcoI YGGCCR 2 cut(s) 727, 1912
AcsI RAATTY 6 cut(s) 456, 641, 845, 1807, 1833, 2104
AcuI CTGAAG 4 cut(s) 806, 1751, 2103, 2247
AfaI GTAC 2 cut(s) 702, 2134
AfiI CCNNNNNNNGG 5 cut(s) 110, 111, 112, 926, 1704
AflII CTTAAG 1 cut(s) 2213
AflIII ACRYGT 1 cut(s) 2336
AhdI GACNNNNNGTC 1 cut(s) 1339
AhlI ACTAGT 1 cut(s) 1789
AjnI CCWGG 3 cut(s) 577, 729, 1702
AjuI GAANNNNNNNTTGG 4 cut(s) 359, 391, 913, 945
Alw21I GWGCWC 3 cut(s) 257, 1013, 2279
Alw26I GTCTC 6 cut(s) 996, 1233, 1510, 1533, 2263, 2342
AlwI GGATC 2 cut(s) 847, 2319
Ama87I CYCGRG 2 cut(s) 110, 2278
AoxI GGCC 2 cut(s) 727, 1912
ApeKI GCWGC 6 cut(s) 291, 772, 775, 989, 1105, 2005
ApoI RAATTY 6 cut(s) 456, 641, 845, 1807, 1833, 2104
Asp700I GAANNNNTTC 2 cut(s) 456, 849
AspS9I GGNCC 2 cut(s) 733, 817
AsuC2I CCSGG 1 cut(s) 2154
AsuHPI GGTGA 3 cut(s) 574, 974, 2044
AsuII TTCGAA 1 cut(s) 1799
AvaI CYCGRG 2 cut(s) 110, 2278
AvaII GGWCC 2 cut(s) 733, 817
AxyI CCTNAGG 1 cut(s) 997
BaeI ACNNNNGTAYC 2 cut(s) 1556, 1589
BalI TGGCCA 1 cut(s) 729
BanII GRGCYC 2 cut(s) 1013, 2370
BbsI GAAGAC 5 cut(s) 649, 1338, 1739, 2091, 2235
Bbv12I GWGCWC 3 cut(s) 257, 1013, 2279
BbvCI CCTCAGC 1 cut(s) 2117
BbvI GCAGC 6 cut(s) 303, 759, 787, 1001, 1117, 1992
BccI CCATC 5 cut(s) 154, 553, 1211, 1409, 1415
BcgI CGANNNNNNTGC 2 cut(s) 947, 981
BciT130I CCWGG 3 cut(s) 579, 731, 1704
BciVI GTATCC 2 cut(s) 330, 1869
BclI TGATCA 1 cut(s) 150
BcnI CCSGG 1 cut(s) 2154
BcoDI GTCTC 6 cut(s) 996, 1233, 1510, 1533, 2263, 2342
BcuI ACTAGT 1 cut(s) 1789
BfaI CTAG 8 cut(s) 203, 821, 944, 1146, 1739, 1790, 2099, 2440
BfmI CTRYAG 2 cut(s) 759, 773
BfrI CTTAAG 1 cut(s) 2213
BfuAI ACCTGC 1 cut(s) 1845
BfuI GTATCC 2 cut(s) 330, 1869
BglII AGATCT 1 cut(s) 1027
BisI GCNGC 6 cut(s) 292, 773, 776, 990, 1106, 2006
BlsI GCNGC 6 cut(s) 293, 774, 777, 991, 1107, 2007
Bme1390I CCNGG 4 cut(s) 579, 731, 1704, 2154
Bme18I GGWCC 2 cut(s) 733, 817
BmeRI GACNNNNNGTC 1 cut(s) 1339
BmeT110I CYCGRG 2 cut(s) 110, 2278
BmgT120I GGNCC 2 cut(s) 733, 817
BmiI GGNNCC 7 cut(s) 144, 735, 1497, 2158, 2168, 2367, 2435
BmrFI CCNGG 4 cut(s) 579, 731, 1704, 2154
BmsI GCATC 6 cut(s) 613, 955, 1001, 1198, 1917, 2103
BpiI GAAGAC 5 cut(s) 649, 1338, 1739, 2091, 2235
BpmI CTGGAG 2 cut(s) 600, 1178
Bpu10I CCTNAGC 4 cut(s) 480, 1463, 2117, 2123
Bpu14I TTCGAA 1 cut(s) 1799
BpuEI CTTGAG 4 cut(s) 257, 875, 1184, 2156
BpuMI CCSGG 1 cut(s) 2154
Bsa29I ATCGAT 1 cut(s) 1302
BsaJI CCNNGG 3 cut(s) 1255, 1703, 1915
Bsc4I CCNNNNNNNGG 5 cut(s) 110, 111, 112, 926, 1704
Bse118I RCCGGY 1 cut(s) 294
Bse1I ACTGG 1 cut(s) 606
Bse21I CCTNAGG 1 cut(s) 997
Bse3DI GCAATG 3 cut(s) 658, 975, 1996
BseBI CCWGG 3 cut(s) 579, 731, 1704
BseCI ATCGAT 1 cut(s) 1302
BseDI CCNNGG 3 cut(s) 1255, 1703, 1915
BseGI GGATG 6 cut(s) 367, 514, 590, 992, 1222, 1420
BseLI CCNNNNNNNGG 5 cut(s) 110, 111, 112, 926, 1704
BseMI GCAATG 3 cut(s) 658, 975, 1996
BseMII CTCAG 5 cut(s) 471, 1761, 2059, 2108, 2398
BseNI ACTGG 1 cut(s) 606
BseRI GAGGAG 1 cut(s) 48
BseXI GCAGC 6 cut(s) 303, 759, 787, 1001, 1117, 1992
BsgI GTGCAG 3 cut(s) 368, 428, 2266
Bsh1236I CGCG 1 cut(s) 1593
BshFI GGCC 2 cut(s) 729, 1914
BshVI ATCGAT 1 cut(s) 1302
BsiHKAI GWGCWC 3 cut(s) 257, 1013, 2279
BsiHKCI CYCGRG 2 cut(s) 110, 2278
BsiSI CCGG 2 cut(s) 295, 2154
BslFI GGGAC 1 cut(s) 719
BslI CCNNNNNNNGG 5 cut(s) 110, 111, 112, 926, 1704
BsmAI GTCTC 6 cut(s) 996, 1233, 1510, 1533, 2263, 2342
BsmFI GGGAC 1 cut(s) 719
BsmI GAATGC 1 cut(s) 1247
BsnI GGCC 2 cut(s) 729, 1914
BsoBI CYCGRG 2 cut(s) 110, 2278
Bsp119I TTCGAA 1 cut(s) 1799
Bsp1286I GDGCHC 4 cut(s) 257, 1013, 2279, 2370
Bsp1407I TGTACA 2 cut(s) 700, 2132
Bsp143I GATC 7 cut(s) 150, 839, 1027, 1091, 1273, 2014, 2311
BspACI CCGC 3 cut(s) 38, 146, 1101
BspANI GGCC 2 cut(s) 729, 1914
BspCNI CTCAG 5 cut(s) 472, 1762, 2058, 2109, 2397
BspDI ATCGAT 1 cut(s) 1302
BspFNI CGCG 1 cut(s) 1593
BspLI GGNNCC 7 cut(s) 144, 735, 1497, 2158, 2168, 2367, 2435
BspMAI CTGCAG 2 cut(s) 763, 777
BspMI ACCTGC 1 cut(s) 1845
BspPI GGATC 2 cut(s) 847, 2319
BspT104I TTCGAA 1 cut(s) 1799
BspTI CTTAAG 1 cut(s) 2213
BsrBI CCGCTC 1 cut(s) 38
BsrDI GCAATG 3 cut(s) 658, 975, 1996
BsrFI RCCGGY 1 cut(s) 294
BsrGI TGTACA 2 cut(s) 700, 2132
BsrI ACTGG 1 cut(s) 606
BssAI RCCGGY 1 cut(s) 294
BssECI CCNNGG 3 cut(s) 1255, 1703, 1915
BssMI GATC 7 cut(s) 150, 839, 1027, 1091, 1273, 2014, 2311
BssT1I CCWWGG 2 cut(s) 1255, 1915
Bst2UI CCWGG 3 cut(s) 579, 731, 1704
Bst4CI ACNGT 9 cut(s) 443, 499, 670, 1081, 1294, 1869, 1931, 2274, 2379
Bst6I CTCTTC 2 cut(s) 1611, 2340
BstAFI CTTAAG 1 cut(s) 2213
BstAPI GCANNNNNTGC 1 cut(s) 1642
BstAUI TGTACA 2 cut(s) 700, 2132
BstBI TTCGAA 1 cut(s) 1799
BstC8I GCNNGC 6 cut(s) 265, 269, 296, 770, 1103, 2010
BstF5I GGATG 6 cut(s) 367, 514, 590, 992, 1222, 1420
BstFNI CGCG 1 cut(s) 1593
BstKTI GATC 7 cut(s) 153, 842, 1030, 1094, 1276, 2017, 2314
BstMAI GTCTC 6 cut(s) 996, 1233, 1510, 1533, 2263, 2342
BstMBI GATC 7 cut(s) 150, 839, 1027, 1091, 1273, 2014, 2311
BstMWI GCNNNNNNNGC 2 cut(s) 1459, 1642
BstNI CCWGG 3 cut(s) 579, 731, 1704
BstNSI RCATGY 2 cut(s) 1462, 2340
BstSCI CCNGG 4 cut(s) 577, 729, 1702, 2152
BstSFI CTRYAG 2 cut(s) 759, 773
BstUI CGCG 1 cut(s) 1593
BstV1I GCAGC 6 cut(s) 303, 759, 787, 1001, 1117, 1992
BstV2I GAAGAC 5 cut(s) 649, 1338, 1739, 2091, 2235
BstX2I RGATCY 2 cut(s) 1027, 2311
BstXI CCANNNNNNTGG 1 cut(s) 1922
BstYI RGATCY 2 cut(s) 1027, 2311
Bsu15I ATCGAT 1 cut(s) 1302
Bsu36I CCTNAGG 1 cut(s) 997
BsuI GTATCC 2 cut(s) 330, 1869
BsuRI GGCC 2 cut(s) 729, 1914
BsuTUI ATCGAT 1 cut(s) 1302
BtgZI GCGATG 1 cut(s) 1434
BtsCI GGATG 6 cut(s) 367, 514, 590, 992, 1222, 1420
BtsI GCAGTG 1 cut(s) 24
BtsIMutI CAGTG 6 cut(s) 24, 390, 599, 1431, 2053, 2279
BveI ACCTGC 1 cut(s) 1845
Cac8I GCNNGC 6 cut(s) 265, 269, 296, 770, 1103, 2010
Cfr10I RCCGGY 1 cut(s) 294
Cfr13I GGNCC 2 cut(s) 733, 817
ClaI ATCGAT 1 cut(s) 1302
CseI GACGC 1 cut(s) 70
Csp6I GTAC 2 cut(s) 701, 2133
CviAII CATG 8 cut(s) 716, 906, 986, 1175, 1459, 1900, 2266, 2337
CviQI GTAC 2 cut(s) 701, 2133
DpnI GATC 7 cut(s) 152, 841, 1029, 1093, 1275, 2016, 2313
DpnII GATC 7 cut(s) 150, 839, 1027, 1091, 1273, 2014, 2311
DraI TTTAAA 1 cut(s) 1812
DriI GACNNNNNGTC 1 cut(s) 1339
EaeI YGGCCR 2 cut(s) 727, 1912
Eam1104I CTCTTC 2 cut(s) 1611, 2340
Eam1105I GACNNNNNGTC 1 cut(s) 1339
EarI CTCTTC 2 cut(s) 1611, 2340
Ecl136II GAGCTC 1 cut(s) 1011
Eco130I CCWWGG 2 cut(s) 1255, 1915
Eco24I GRGCYC 2 cut(s) 1013, 2370
Eco32I GATATC 1 cut(s) 118
Eco47I GGWCC 2 cut(s) 733, 817
Eco53kI GAGCTC 1 cut(s) 1011
Eco57I CTGAAG 4 cut(s) 806, 1751, 2103, 2247
Eco81I CCTNAGG 1 cut(s) 997
Eco88I CYCGRG 2 cut(s) 110, 2278
EcoICRI GAGCTC 1 cut(s) 1011
EcoO109I RGGNCCY 1 cut(s) 733
EcoRI GAATTC 1 cut(s) 845
EcoRII CCWGG 3 cut(s) 577, 729, 1702
EcoRV GATATC 1 cut(s) 118
EcoT14I CCWWGG 2 cut(s) 1255, 1915
EcoT38I GRGCYC 2 cut(s) 1013, 2370
ErhI CCWWGG 2 cut(s) 1255, 1915
FaeI CATG 8 cut(s) 719, 909, 989, 1178, 1462, 1903, 2269, 2340
FalI AAGNNNNNCTT 4 cut(s) 524, 556, 671, 703
FaqI GGGAC 1 cut(s) 719
FatI CATG 8 cut(s) 715, 905, 985, 1174, 1458, 1899, 2265, 2336
FauI CCCGC 2 cut(s) 31, 1094
FbaI TGATCA 1 cut(s) 150
FblI GTMKAC 1 cut(s) 1392
Fnu4HI GCNGC 6 cut(s) 292, 773, 776, 990, 1106, 2006
FokI GGATG 6 cut(s) 374, 521, 597, 979, 1229, 1427
FriOI GRGCYC 2 cut(s) 1013, 2370
Fsp4HI GCNGC 6 cut(s) 292, 773, 776, 990, 1106, 2006
FspBI CTAG 8 cut(s) 203, 821, 944, 1146, 1739, 1790, 2099, 2440
GluI GCNGC 6 cut(s) 292, 773, 776, 990, 1106, 2006
GsuI CTGGAG 2 cut(s) 600, 1178
HaeIII GGCC 2 cut(s) 729, 1914
HapII CCGG 2 cut(s) 295, 2154
HgaI GACGC 1 cut(s) 70
Hin1II CATG 8 cut(s) 719, 909, 989, 1178, 1462, 1903, 2269, 2340
HpaII CCGG 2 cut(s) 295, 2154
HphI GGTGA 3 cut(s) 574, 974, 2044
Hpy166II GTNNAC 1 cut(s) 1393
Hpy8I GTNNAC 1 cut(s) 1393
HpyAV CCTTC 8 cut(s) 82, 169, 224, 364, 413, 1854, 2307, 2380
HpyCH4III ACNGT 9 cut(s) 443, 499, 670, 1081, 1294, 1869, 1931, 2274, 2379
HpyCH4IV ACGT 1 cut(s) 1051
HpyF10VI GCNNNNNNNGC 2 cut(s) 1459, 1642
HpySE526I ACGT 1 cut(s) 1051
Hsp92II CATG 8 cut(s) 719, 909, 989, 1178, 1462, 1903, 2269, 2340
KroI GCCGGC 1 cut(s) 294
KroNI GCCGGC 1 cut(s) 296
Ksp22I TGATCA 1 cut(s) 150
Kzo9I GATC 7 cut(s) 150, 839, 1027, 1091, 1273, 2014, 2311
LmnI GCTCC 9 cut(s) 35, 91, 238, 580, 1008, 2002, 2162, 2365, 2439
Lsp1109I GCAGC 6 cut(s) 303, 759, 787, 1001, 1117, 1992
LweI GCATC 6 cut(s) 613, 955, 1001, 1198, 1917, 2103
MaeI CTAG 8 cut(s) 203, 821, 944, 1146, 1739, 1790, 2099, 2440
MaeII ACGT 1 cut(s) 1051
MalI GATC 7 cut(s) 152, 841, 1029, 1093, 1275, 2016, 2313
MbiI CCGCTC 1 cut(s) 38
MboI GATC 7 cut(s) 150, 839, 1027, 1091, 1273, 2014, 2311
MfeI CAATTG 1 cut(s) 2033
MflI RGATCY 2 cut(s) 1027, 2311
MhlI GDGCHC 4 cut(s) 257, 1013, 2279, 2370
MlsI TGGCCA 1 cut(s) 729
MluNI TGGCCA 1 cut(s) 729
MlyI GAGTC 3 cut(s) 128, 1175, 1729
MmeI TCCRAC 4 cut(s) 481, 512, 1066, 1504
Mox20I TGGCCA 1 cut(s) 729
MroNI GCCGGC 1 cut(s) 294
MroXI GAANNNNTTC 2 cut(s) 456, 849
MscI TGGCCA 1 cut(s) 729
MseI TTAA 7 cut(s) 78, 303, 527, 872, 1811, 2180, 2214
MslI CAYNNNNRTG 2 cut(s) 1304, 1630
Msp20I TGGCCA 1 cut(s) 729
MspA1I CMGCKG 1 cut(s) 148
MspCI CTTAAG 1 cut(s) 2213
MspI CCGG 2 cut(s) 295, 2154
MspR9I CCNGG 4 cut(s) 579, 731, 1704, 2154
MunI CAATTG 1 cut(s) 2033
Mva1269I GAATGC 1 cut(s) 1247
MvaI CCWGG 3 cut(s) 579, 731, 1704
MvnI CGCG 1 cut(s) 1593
MwoI GCNNNNNNNGC 2 cut(s) 1459, 1642
NaeI GCCGGC 1 cut(s) 296
NciI CCSGG 1 cut(s) 2154
NdeII GATC 7 cut(s) 150, 839, 1027, 1091, 1273, 2014, 2311
NgoMIV GCCGGC 1 cut(s) 294
NlaIII CATG 8 cut(s) 719, 909, 989, 1178, 1462, 1903, 2269, 2340
NlaIV GGNNCC 7 cut(s) 144, 735, 1497, 2158, 2168, 2367, 2435
NmuCI GTSAC 4 cut(s) 315, 2021, 2050, 2332
NspI RCATGY 2 cut(s) 1462, 2340
NspV TTCGAA 1 cut(s) 1799
PaeR7I CTCGAG 1 cut(s) 2278
PciI ACATGT 1 cut(s) 2336
PctI GAATGC 1 cut(s) 1247
PdiI GCCGGC 1 cut(s) 296
PdmI GAANNNNTTC 2 cut(s) 456, 849
PfeI GAWTC 7 cut(s) 71, 208, 414, 1281, 1351, 1796, 1818
PflMI CCANNNNNTGG 1 cut(s) 926
PfoI TCCNGGA 1 cut(s) 577
PkrI GCNGC 6 cut(s) 293, 774, 777, 991, 1107, 2007
PleI GAGTC 3 cut(s) 128, 1174, 1729
PpsI GAGTC 3 cut(s) 128, 1174, 1729
PpuMI RGGWCCY 1 cut(s) 733
PscI ACATGT 1 cut(s) 2336
Psp124BI GAGCTC 1 cut(s) 1013
Psp1406I AACGTT 1 cut(s) 1051
Psp5II RGGWCCY 1 cut(s) 733
Psp6I CCWGG 3 cut(s) 577, 729, 1702
PspGI CCWGG 3 cut(s) 577, 729, 1702
PspN4I GGNNCC 7 cut(s) 144, 735, 1497, 2158, 2168, 2367, 2435
PspPI GGNCC 2 cut(s) 733, 817
PspPPI RGGWCCY 1 cut(s) 733
PstI CTGCAG 2 cut(s) 763, 777
PsuI RGATCY 2 cut(s) 1027, 2311
RsaI GTAC 2 cut(s) 702, 2134
RsaNI GTAC 2 cut(s) 701, 2133
RseI CAYNNNNRTG 2 cut(s) 1304, 1630
SacI GAGCTC 1 cut(s) 1013
SaqAI TTAA 7 cut(s) 78, 303, 527, 872, 1811, 2180, 2214
SatI GCNGC 6 cut(s) 292, 773, 776, 990, 1106, 2006
Sau3AI GATC 7 cut(s) 150, 839, 1027, 1091, 1273, 2014, 2311
Sau96I GGNCC 2 cut(s) 733, 817
SchI GAGTC 3 cut(s) 128, 1175, 1729
ScrFI CCNGG 4 cut(s) 579, 731, 1704, 2154
SduI GDGCHC 4 cut(s) 257, 1013, 2279, 2370
SfaNI GCATC 6 cut(s) 613, 955, 1001, 1198, 1917, 2103
SfcI CTRYAG 2 cut(s) 759, 773
Sfr274I CTCGAG 1 cut(s) 2278
SfuI TTCGAA 1 cut(s) 1799
SinI GGWCC 2 cut(s) 733, 817
SlaI CTCGAG 1 cut(s) 2278
SmiMI CAYNNNNRTG 2 cut(s) 1304, 1630
SmlI CTYRAG 6 cut(s) 272, 854, 1163, 2171, 2213, 2278
SmoI CTYRAG 6 cut(s) 272, 854, 1163, 2171, 2213, 2278
SpeI ACTAGT 1 cut(s) 1789
SsiI CCGC 3 cut(s) 38, 146, 1101
SspI AATATT 1 cut(s) 1978
SspMI CTAG 8 cut(s) 203, 821, 944, 1146, 1739, 1790, 2099, 2440
SstI GAGCTC 1 cut(s) 1013
StyD4I CCNGG 4 cut(s) 577, 729, 1702, 2152
StyI CCWWGG 2 cut(s) 1255, 1915
TaaI ACNGT 9 cut(s) 443, 499, 670, 1081, 1294, 1869, 1931, 2274, 2379
TaiI ACGT 1 cut(s) 1054
TaqI TCGA 8 cut(s) 257, 417, 557, 1302, 1607, 1799, 1847, 2279
TatI WGTACW 2 cut(s) 700, 2132
TfiI GAWTC 7 cut(s) 71, 208, 414, 1281, 1351, 1796, 1818
Tru1I TTAA 7 cut(s) 78, 303, 527, 872, 1811, 2180, 2214
Tru9I TTAA 7 cut(s) 78, 303, 527, 872, 1811, 2180, 2214
TscAI CASTG 6 cut(s) 24, 390, 606, 1438, 2053, 2279
TseFI GTSAC 4 cut(s) 315, 2021, 2050, 2332
TseI GCWGC 6 cut(s) 291, 772, 775, 989, 1105, 2005
Tsp45I GTSAC 4 cut(s) 315, 2021, 2050, 2332
TspGWI ACGGA 2 cut(s) 564, 2062
TspRI CASTG 6 cut(s) 24, 390, 606, 1438, 2053, 2279
Van91I CCANNNNNTGG 1 cut(s) 926
Vha464I CTTAAG 1 cut(s) 2213
VpaK11BI GGWCC 2 cut(s) 733, 817
XapI RAATTY 6 cut(s) 456, 641, 845, 1807, 1833, 2104
XbaI TCTAGA 2 cut(s) 943, 1145
XceI RCATGY 2 cut(s) 1462, 2340
XcmI CCANNNNNNNNNTGG 1 cut(s) 1116
XhoI CTCGAG 1 cut(s) 2278
XmiI GTMKAC 1 cut(s) 1392
XmnI GAANNNNTTC 2 cut(s) 456, 849
XspI CTAG 8 cut(s) 203, 821, 944, 1146, 1739, 1790, 2099, 2440
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.