RchiOBHm_Chr3g0480561

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0000039
Physical Location & Seq
Reverse (-)
26360814 .. 26364197
3384 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ44557

Sequence Viewer

Length: 2523 bp
ATGTTTGATTTTTTCTTGGTAAATTTGAAGTTTTTATTTATTTTGGTGTCTATTGGGTGGTGTTGTATGATGACAGTGGTGATAATCTGCGGAGCTGGTTTAGCGGTGACATTTTGGGCGCTGTGTGATTCTGTTAAGCAAGTGAAGGACCAGATACTGATATTCAAACCCCAACTCGGGGATATCACGTCCACTCTGGACTCTTTGCAGCCCCTGATCAAGGAGATGGCACAGTGTGAGAAGGAAATGGACGGTCCAGAGGAGGAACTAGAGAATCTTAGAGTGGAAATGGAGGAAGGAGTGGAGCTTATTCGCAAGTGTTCGGAAGCGAGCAGATTCAAGAAGTATAAGTATGGCAGCAGACTTGTTGAATTGAGCAAGTCACTTCAGAGGCTGTTGGAGATATTGAAAGTGCAGGGCATAAGGGATGTGAAGAAGGCATTGGTTTCGGTGAGGAACATTGAAAATGTGGTGCACAGAATTGAAGGGAATTGTGTGATACAGAACAATCAGTCTGAAAAAATTGAAGGGTGGTGTGCTGTGCCTGAGCCTCCAACAATTACGGTTGGATTGGATTTGCCTTTGAGGGAATTGAAGATGAAGCTCTTTAAGGACGGAATGTCGATGGTGATACTTACTGCTCCAGGAGGATGTGGGAAAACTACACTGGCAACAAAATTATGTCATGATGCAGAAGTCAAAGATAAATTCAAGAGCAATGTCTTCTTTGTTACTGTTTCAAAGAAGCCCAACTTATACCTAATTGTAAAAGAGCTATATCATCGAACGGGTGCCCAGGTCCCCGATTTCCAAAGTGAAGTAACTGCAGTTAGCTGGCTGCAGCAATTTCTGAAGCTAACAGGACAAAATCCTATGTTGTTGATCCTGGATGATGTTTGGGCTGGATCAGAATTCATTCTTGAGAAGTTCGATGAATTAAAATTGACAAATTACAAGATATTGGTGACATCAAGGTCTGCTTTTCCAAGATTTGGTTCTCCATATTATTTAGAATGTTTGAACCGCAAAGATGCAATGGCACTTTTTCACCATGCAGCATCCTTAGGAGACAGGAGCTCTTATATTTCAGAAGATCTTTCAACAAAGATAGTAGAACGTTGTATGGGATTTCCACTTGCAATTACAGTTGTCGGAAGATCACTTTGCGGGCAGCCAATAGAGATTTGGCAAAAAAGAGTAATGGAATGGTCTAGAGGTTCTTCTGTTCTTGAGTCTGAACATGATTTGCTTGATTGCCTCCAAAGTAGTTTAGATGTTTTGTATGCAGAGAAGCCTCTTATGAGGGAATGCTTTATAGACCTTGGTTTATTTCCTGAAGATCAAAGAATTCCTACCACTGTCCTGATTGATATGTGGGCAGAGTTATATGGCATACATGAAGACATTTCGTCCATTGCGAACCTCTATGAACTTGCCTCTCGAAGTTTGGCTAATCTGGTAGTCACAAGGAAGGACAACAAGGATAGTGATGGCTATTACACTGAACACTTCGTTACACAGCATGACATGCTTAGGGAGTTGGCTATCCAGCAGTTGAGTCAGGAACCACTACAACATAGAATGAGGCTTATGATTGACATATGTGGAGACAACCTTCCTATGTGGTGGAAAGAACAAAAGTATCAATCCCTCATTGCTCGCCTTGTATCGATCTCGACAGATGAAGAGTTCTCATCTAAATGGCAAAACTTGCAACTACCAGAAGCAGAGGTTTTGATTTTGAACTTTCAGACCAAGTATTATGCCTTACCTGAGTCTGTGGAGAAAATGAATAAATTGAAGACTCTAGTACTCACTAGTTACGGTATCAGTCCTGCTGAGTTGAATAACTTTCAACTAGTTGATTCAAAACCGAATCTGAAGAGAATCAGATTAGAACGAATTTCAATTCCTTCCATAAGCAGGTATCCTATACAGTTGAAAAGTTTGAAAAAGATTTCTCTATTCATGTGTAGCATCGGCCAAGGTTTTGGTAATGGTTCCATCCATATATCAGAGGCGTTTCCAAATCTTGTTGAAATGAATATTGATTACTGCAATGATTTGGTGGAGTTGCCTCCCCAATTGTGTGACCTTATTCAATTGAAGAAACTCAGTGTCACCAATTCTCATAAACTCTCTGCCTTACCTGAAGACATTGGAAATCTAGTAAATTTAGAGCTGTTGAGGCTCAGGTGTTGTACAGACTTGTCAGAATTGCCCGGCTCGATTAGGAACCTCAAGAAGTTAAATTTGCTTGACATATCTGATTGCTTTAGCATTAAGGAATTGCCTGAAGACATTGGAGAATTGTGCAATTTAGAAAGGCTCAACATGAGACAGTGCTCGAGATTGAATGAGTTGCCTTCTTCAGTTTTGAATCTTGAACAGTTGAAGGAAGTGACATGTGATGAAGAGACTGAAATTTTATGGGAACCCTTTTTACCCTTTCTCAAGAACATGCACATGAAGGCTGTGAAAGAAGATATGAACCTGAATTGGCTCCACAAGGCACATTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

840

Amino Acids

96.04

Weight (kDa)

5.56

Isoelectric Point (pI)

45.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 28 - 153 9.1e-23 Arabidopsis broad-spectrum mildew resistance protein RPW8
NB-ARC PF00931 195 - 350 3.9e-15 NB-ARC domain
AAA_22 PF13401 208 - 309 4.1e-06 AAA domain
LRR_14 PF23598 675 - 757 7.4e-09 Leucine-rich repeat region
LRR_14 PF23598 760 - 809 3.1e-07 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000039)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66890 AT5G66900 AT5G66900 AT5G66900 AT5G66910
fragaria_vesca FvH4_1g15090 FvH4_1g15120 FvH4_1g15130 FvH4_1g15140 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15170 FvH4_1g15171 FvH4_1g15171 FvH4_1g15200 FvH4_1g15200 FvH4_1g15200 FvH4_1g15210 FvH4_1g15210 FvH4_1g15210 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15230 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15500 FvH4_1g15500 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_2g19760 FvH4_3g12820 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_6g18970 FvH4_6g42370 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g50100 FvH4_6g50100 FvH4_6g50110 FvH4_6g50130 FvH4_6g50140 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50330
malus_domestica MD00G1053700.v1.1 MD02G1164300.v1.1 MD02G1164400.v1.1 MD02G1164700.v1.1 MD02G1164800.v1.1 MD02G1165300.v1.1 MD02G1165400.v1.1 MD02G1165500.v1.1 MD02G1165600.v1.1 MD02G1165900.v1.1 MD02G1166000.v1.1 MD02G1166100.v1.1 MD02G1166300.v1.1 MD08G1084900.v1.1 MD15G1263100.v1.1 MD15G1263300.v1.1 MD15G1276900.v1.1 MD15G1277200.v1.1 MD15G1277400.v1.1 MD15G1277700.v1.1 MD15G1277800.v1.1 MD15G1278000.v1.1 MD15G1278100.v1.1 MD15G1278400.v1.1 MD15G1278600.v1.1
prunus_persica Prupe.7G138500_v2.0.a1 Prupe.7G138500_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138700_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138900_v2.0.a1 Prupe.7G139100_v2.0.a1 Prupe.7G139200_v2.0.a1 Prupe.7G139500_v2.0.a1 Prupe.7G139600_v2.0.a1 Prupe.7G139700_v2.0.a1 Prupe.7G139800_v2.0.a1
pyrus_communis pycom02g13150 pycom02g13160 pycom02g13190 pycom02g13200 pycom02g13220 pycom02g13230 pycom02g13250 pycom02g13260 pycom02g13270 pycom02g13300 pycom02g13310 pycom02g13330 pycom15g23980 pycom15g23990 pycom15g24030 pycom15g24040 pycom15g24050 pycom15g24070 pycom15g24090 pycom15g24160
rosa_chinensis RchiOBHm_Chr1g0326431 RchiOBHm_Chr1g0326451 RchiOBHm_Chr2g0104121 RchiOBHm_Chr2g0104151 RchiOBHm_Chr2g0104171 RchiOBHm_Chr2g0104181 RchiOBHm_Chr2g0104191 RchiOBHm_Chr2g0104201 RchiOBHm_Chr2g0104211 RchiOBHm_Chr2g0104241 RchiOBHm_Chr2g0104261 RchiOBHm_Chr2g0104281 RchiOBHm_Chr2g0104311 RchiOBHm_Chr2g0104331 RchiOBHm_Chr2g0104361 RchiOBHm_Chr2g0104381 RchiOBHm_Chr2g0104391 RchiOBHm_Chr2g0104431 RchiOBHm_Chr2g0104441 RchiOBHm_Chr2g0104541 RchiOBHm_Chr2g0104611 RchiOBHm_Chr2g0104741 RchiOBHm_Chr2g0104761 RchiOBHm_Chr2g0158291 RchiOBHm_Chr2g0170371 RchiOBHm_Chr2g0170631 RchiOBHm_Chr3g0473971 RchiOBHm_Chr3g0480491 RchiOBHm_Chr3g0480541 RchiOBHm_Chr3g0480561 RchiOBHm_Chr3g0485281 RchiOBHm_Chr3g0485291 RchiOBHm_Chr4g0411541 RchiOBHm_Chr6g0285471
rosa_laevigata RLG00000008347 RLG00000012637 RLG00000016961 RLG00000017293 RLG00000017305 RLG00000017308 RLG00000017312 RLG00000017314 RLG00000017317 RLG00000017318 RLG00000017319 RLG00000017321 RLG00000017322 RLG00000017323 RLG00000017324 RLG00000017325 RLG00000017327 RLG00000017328 RLG00000017329 RLG00000017330 RLG00000017334 RLG00000020833 RLG00000021073 RLG00000021950 RLG00000021971 RLG00000023154 RLG00000023157 RLG00000023467 RLG00000023468 RLG00000023525 RLG00000023963 RLG00000033265 RLG00000033267 RLG00000033268
rosa_multiflora Rmu_co8053082.1_g000001 Rmu_co8210358.1_g000001 Rmu_co8331661.1_g000001 Rmu_co8477427.1_g000002 Rmu_co8483217.1_g000001 Rmu_sc0000284.1_g000004 Rmu_sc0000676.1_g000017 Rmu_sc0000676.1_g000019 Rmu_sc0000676.1_g000021 Rmu_sc0002205.1_g000038 Rmu_sc0002205.1_g000041 Rmu_sc0002316.1_g000010 Rmu_sc0002316.1_g000012 Rmu_sc0002316.1_g000040 Rmu_sc0002316.1_g000071 Rmu_sc0002316.1_g000072 Rmu_sc0002316.1_g000095 Rmu_sc0002316.1_g000102 Rmu_sc0002414.1_g000018 Rmu_sc0002576.1_g000002 Rmu_sc0002576.1_g000003 Rmu_sc0002687.1_g000010 Rmu_sc0002687.1_g000017 Rmu_sc0003660.1_g000001 Rmu_sc0003787.1_g000014 Rmu_sc0003787.1_g000020 Rmu_sc0003787.1_g000024 Rmu_sc0003787.1_g000029 Rmu_sc0004059.1_g000026 Rmu_sc0005137.1_g000021 Rmu_sc0006550.1_g000001 Rmu_sc0006561.1_g000025 Rmu_sc0006561.1_g000033 Rmu_sc0007704.1_g000005 Rmu_sc0008904.1_g000005 Rmu_sc0010270.1_g000002 Rmu_sc0010307.1_g000001 Rmu_sc0011601.1_g000013 Rmu_sc0012694.1_g000005 Rmu_sc0013101.1_g000008 Rmu_sc0019029.1_g000001 Rmu_sc0019728.1_g000001 Rmu_sc0025829.1_g000001 Rmu_sc0025829.1_g000002 Rmu_ssc0000257.1_g000007 Rmu_ssc0000257.1_g000008
rosa_roxburghii Rroxscaffold_1G00021710 Rroxscaffold_1G00049350 Rroxscaffold_1G00049360 Rroxscaffold_2G00081160 Rroxscaffold_2G00081360 Rroxscaffold_2G00081390 Rroxscaffold_2G00091540 Rroxscaffold_2G00138790 Rroxscaffold_2G00138800 Rroxscaffold_2G00138920 Rroxscaffold_2G00139000 Rroxscaffold_2G00139010 Rroxscaffold_2G00139020 Rroxscaffold_2G00139030 Rroxscaffold_2G00139040 Rroxscaffold_2G00139050 Rroxscaffold_2G00139060 Rroxscaffold_2G00139070 Rroxscaffold_2G00139090 Rroxscaffold_2G00139100 Rroxscaffold_3G00257470 Rroxscaffold_4G00318040 Rroxscaffold_4G00323740 Rroxscaffold_4G00323750 Rroxscaffold_5G00356140 Rroxscaffold_6G00401280 Rroxscaffold_6G00401290 Rroxscaffold_7G00183120
rosa_rugosa Rorug01G0060500 Rorug01G0060900 Rorug02G0119500 Rorug02G0119600 Rorug02G0119700 Rorug02G0119700 Rorug02G0119700 Rorug02G0119800.1 Rorug02G0119900.1 Rorug02G0120000 Rorug02G0120100 Rorug02G0120200 Rorug02G0120300 Rorug02G0120400 Rorug02G0120500 Rorug02G0120700 Rorug02G0120700 Rorug02G0122300 Rorug02G0122300 Rorug02G0122400.1 Rorug02G0466700 Rorug02G0466700 Rorug02G0466800 Rorug02G0548600 Rorug02G0549900 Rorug02G0550000 Rorug03G0184200 Rorug04G0107900 Rorug05G0121500 Rorug06G0174800
rosa_samantha Rh1AG198400 Rh1CG074700 Rh1CG075000 Rh1CG183700 Rh2AG169700 Rh2AG169800 Rh2AG169900 Rh2AG170000 Rh2AG170300 Rh2AG170400 Rh2AG171000 Rh2AG171100 Rh2AG171600 Rh2AG172400 Rh2AG172500 Rh2AG532900 Rh2AG620000 Rh2AG622500 Rh2AG622800 Rh2AG623000 Rh2BG176200 Rh2BG176300 Rh2BG176400 Rh2BG176500 Rh2BG176600 Rh2BG176900 Rh2BG177000 Rh2BG177100 Rh2BG177200 Rh2BG177300 Rh2BG177600 Rh2BG177700 Rh2BG177900 Rh2BG178000 Rh2BG178200 Rh2BG178300 Rh2BG178500 Rh2BG179000 Rh2BG180000 Rh2BG180100 Rh2BG259200 Rh2BG546700 Rh2BG630800 Rh2BG633500 Rh2CG176500 Rh2CG177500 Rh2CG177700 Rh2DG174400 Rh2DG174600 Rh2DG174900 Rh2DG175100 Rh2DG175200 Rh2DG175300 Rh2DG175600 Rh2DG175700 Rh2DG175800 Rh2DG175900 Rh2DG176000 Rh2DG176300 Rh2DG176400 Rh2DG176500 Rh2DG176600 Rh2DG176900 Rh2DG177100 Rh2DG177200 Rh2DG177800 Rh2DG177900 Rh2DG178700 Rh2DG178800 Rh2DG555700 Rh2DG643700 Rh2DG646300 Rh3AG235600 Rh3AG235900 Rh3AG236200 Rh3AG266700 Rh3AG266900 Rh3BG216200 Rh3BG269100 Rh3CG212700 Rh3CG265800 Rh4AG168900 Rh4BG165500 Rh4CG180300 Rh5BG261500 Rh6AG286300 Rh6BG287500 Rh6CG288600 Rh7AG022600
rosa_wichuraiana Rw0G008240 Rw0G008250 Rw0G013190 Rw0G013200 Rw2G013230 Rw2G013240 Rw2G013270 Rw2G013280 Rw2G013290 Rw2G013300 Rw2G013330 Rw2G013340 Rw2G013350 Rw2G013360 Rw2G013370 Rw2G013380 Rw2G013410 Rw2G013570 Rw2G044120 Rw2G051370 Rw2G051590 Rw3G017160 Rw3G021150 Rw3G021160 Rw3G023730 Rw3G023740 Rw4G014030 Rw6G024590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 973
Acc36I ACCTGC 1 cut(s) 1914
AccB1I GGYRCC 1 cut(s) 791
AccB7I CCANNNNNTGG 1 cut(s) 992
AciI CCGC 4 cut(s) 90, 104, 1024, 1167
AclI AACGTT 1 cut(s) 1117
AclWI GGATC 2 cut(s) 877, 913
AcoI YGGCCR 1 cut(s) 1981
AcsI RAATTY 8 cut(s) 22, 707, 911, 1347, 1902, 2173, 2251, 2424
AcuI CTGAAG 7 cut(s) 371, 872, 1356, 1901, 2172, 2316, 2355
AdeI CACNNNGTG 1 cut(s) 236
AfaI GTAC 2 cut(s) 1812, 2203
AfiI CCNNNNNNNGG 6 cut(s) 176, 177, 178, 220, 992, 1923
AflIII ACRYGT 1 cut(s) 2405
AhdI GACNNNNNGTC 2 cut(s) 619, 1408
AhlI ACTAGT 2 cut(s) 1817, 1858
AjiI CACGTC 1 cut(s) 189
AjnI CCWGG 3 cut(s) 643, 795, 885
AjuI GAANNNNNNNTTGG 4 cut(s) 425, 457, 979, 1011
AluBI AGCT 8 cut(s) 95, 307, 604, 775, 834, 856, 1077, 2182
AluI AGCT 8 cut(s) 95, 307, 604, 775, 834, 856, 1077, 2182
Alw21I GWGCWC 3 cut(s) 477, 1079, 2348
Alw26I GTCTC 4 cut(s) 1062, 1602, 2332, 2411
Alw44I GTGCAC 1 cut(s) 473
AlwI GGATC 2 cut(s) 877, 913
AlwNI CAGNNNCTG 3 cut(s) 157, 214, 394
Ama87I CYCGRG 2 cut(s) 176, 2347
AoxI GGCC 1 cut(s) 1981
ApaLI GTGCAC 1 cut(s) 473
ApeKI GCWGC 6 cut(s) 208, 357, 838, 841, 1055, 1171
ApoI RAATTY 8 cut(s) 22, 707, 911, 1347, 1902, 2173, 2251, 2424
Asp700I GAANNNNTTC 1 cut(s) 915
AspLEI GCGC 1 cut(s) 121
AspS9I GGNCC 3 cut(s) 148, 254, 799
AsuC2I CCSGG 1 cut(s) 2223
AsuHPI GGTGA 7 cut(s) 91, 118, 463, 640, 976, 1040, 2113
AvaI CYCGRG 2 cut(s) 176, 2347
AvaII GGWCC 3 cut(s) 148, 254, 799
AxyI CCTNAGG 1 cut(s) 1063
BaeGI GKGCMC 2 cut(s) 477, 796
BaeI ACNNNNGTAYC 2 cut(s) 1625, 1658
BanI GGYRCC 1 cut(s) 791
BanII GRGCYC 1 cut(s) 1079
BbsI GAAGAC 5 cut(s) 715, 1407, 1808, 2160, 2304
Bbv12I GWGCWC 3 cut(s) 477, 1079, 2348
BbvI GCAGC 6 cut(s) 220, 369, 825, 853, 1067, 1183
BccI CCATC 4 cut(s) 220, 619, 1484, 2012
BcgI CGANNNNNNTGC 2 cut(s) 429, 463
BciT130I CCWGG 3 cut(s) 645, 797, 887
BciVI GTATCC 1 cut(s) 1938
BclI TGATCA 1 cut(s) 216
BcnI CCSGG 1 cut(s) 2223
BcoDI GTCTC 4 cut(s) 1062, 1602, 2332, 2411
BcuI ACTAGT 2 cut(s) 1817, 1858
BfaI CTAG 6 cut(s) 269, 1212, 1808, 1818, 1859, 2168
BfmI CTRYAG 2 cut(s) 825, 839
BfoI RGCGCY 1 cut(s) 122
BfuAI ACCTGC 1 cut(s) 1914
BfuI GTATCC 1 cut(s) 1938
BglII AGATCT 1 cut(s) 1093
BisI GCNGC 6 cut(s) 209, 358, 839, 842, 1056, 1172
BlsI GCNGC 6 cut(s) 210, 359, 840, 843, 1057, 1173
BmcAI AGTACT 1 cut(s) 1812
Bme1390I CCNGG 4 cut(s) 645, 797, 887, 2223
Bme18I GGWCC 3 cut(s) 148, 254, 799
BmeRI GACNNNNNGTC 2 cut(s) 619, 1408
BmeT110I CYCGRG 2 cut(s) 176, 2347
BmgBI CACGTC 1 cut(s) 189
BmgT120I GGNCC 3 cut(s) 148, 254, 799
BmiI GGNNCC 7 cut(s) 793, 801, 1566, 2002, 2237, 2436, 2504
BmrFI CCNGG 4 cut(s) 645, 797, 887, 2223
BmsI GCATC 4 cut(s) 679, 1021, 1067, 1986
BpiI GAAGAC 5 cut(s) 715, 1407, 1808, 2160, 2304
BpmI CTGGAG 1 cut(s) 627
Bpu10I CCTNAGC 3 cut(s) 546, 1532, 2192
BpuEI CTTGAG 4 cut(s) 941, 1250, 2225, 2438
BpuMI CCSGG 1 cut(s) 2223
Bsa29I ATCGAT 1 cut(s) 1670
BsaJI CCNNGG 3 cut(s) 795, 1321, 1984
Bsc4I CCNNNNNNNGG 6 cut(s) 176, 177, 178, 220, 992, 1923
Bse1I ACTGG 1 cut(s) 672
Bse21I CCTNAGG 1 cut(s) 1063
Bse3DI GCAATG 6 cut(s) 724, 1041, 1413, 1653, 2065, 2515
BseBI CCWGG 3 cut(s) 645, 797, 887
BseCI ATCGAT 1 cut(s) 1670
BseDI CCNNGG 3 cut(s) 795, 1321, 1984
BseGI GGATG 5 cut(s) 433, 656, 895, 1058, 2004
BseLI CCNNNNNNNGG 6 cut(s) 176, 177, 178, 220, 992, 1923
BseMI GCAATG 6 cut(s) 724, 1041, 1413, 1653, 2065, 2515
BseMII CTCAG 5 cut(s) 537, 1764, 1830, 2128, 2206
BseNI ACTGG 1 cut(s) 672
BseRI GAGGAG 1 cut(s) 275
BseSI GKGCMC 2 cut(s) 477, 796
BseXI GCAGC 6 cut(s) 220, 369, 825, 853, 1067, 1183
BsgI GTGCAG 1 cut(s) 434
BshFI GGCC 1 cut(s) 1983
BshNI GGYRCC 1 cut(s) 791
BshVI ATCGAT 1 cut(s) 1670
BsiHKAI GWGCWC 3 cut(s) 477, 1079, 2348
BsiHKCI CYCGRG 2 cut(s) 176, 2347
BsiSI CCGG 1 cut(s) 2223
BslFI GGGAC 1 cut(s) 785
BslI CCNNNNNNNGG 6 cut(s) 176, 177, 178, 220, 992, 1923
BsmAI GTCTC 4 cut(s) 1062, 1602, 2332, 2411
BsmFI GGGAC 1 cut(s) 785
BsmI GAATGC 1 cut(s) 1313
BsnI GGCC 1 cut(s) 1983
BsoBI CYCGRG 2 cut(s) 176, 2347
Bsp1286I GDGCHC 4 cut(s) 477, 796, 1079, 2348
Bsp1407I TGTACA 1 cut(s) 2201
Bsp143I GATC 7 cut(s) 216, 882, 905, 1093, 1157, 1339, 1671
BspACI CCGC 4 cut(s) 90, 104, 1024, 1167
BspANI GGCC 1 cut(s) 1983
BspCNI CTCAG 5 cut(s) 538, 1765, 1831, 2127, 2205
BspDI ATCGAT 1 cut(s) 1670
BspHI TCATGA 1 cut(s) 685
BspLI GGNNCC 7 cut(s) 793, 801, 1566, 2002, 2237, 2436, 2504
BspMAI CTGCAG 2 cut(s) 829, 843
BspMI ACCTGC 1 cut(s) 1914
BspPI GGATC 2 cut(s) 877, 913
BspT107I GGYRCC 1 cut(s) 791
BsrDI GCAATG 6 cut(s) 724, 1041, 1413, 1653, 2065, 2515
BsrGI TGTACA 1 cut(s) 2201
BsrI ACTGG 1 cut(s) 672
BssECI CCNNGG 3 cut(s) 795, 1321, 1984
BssMI GATC 7 cut(s) 216, 882, 905, 1093, 1157, 1339, 1671
BssT1I CCWWGG 2 cut(s) 1321, 1984
Bst2UI CCWGG 3 cut(s) 645, 797, 887
Bst6I CTCTTC 3 cut(s) 1680, 1877, 2409
BstAPI GCANNNNNTGC 2 cut(s) 1528, 1711
BstAUI TGTACA 1 cut(s) 2201
BstC8I GCNNGC 4 cut(s) 331, 836, 1169, 1660
BstDEI CTNAG 8 cut(s) 278, 546, 1063, 1532, 1773, 1839, 2114, 2192
BstENI CCTNNNNNAGG 1 cut(s) 218
BstF5I GGATG 5 cut(s) 433, 656, 895, 1058, 2004
BstH2I RGCGCY 1 cut(s) 122
BstHHI GCGC 1 cut(s) 121
BstKTI GATC 7 cut(s) 219, 885, 908, 1096, 1160, 1342, 1674
BstMAI GTCTC 4 cut(s) 1062, 1602, 2332, 2411
BstMBI GATC 7 cut(s) 216, 882, 905, 1093, 1157, 1339, 1671
BstMWI GCNNNNNNNGC 4 cut(s) 101, 1528, 1711, 2188
BstNI CCWGG 3 cut(s) 645, 797, 887
BstNSI RCATGY 3 cut(s) 1531, 2409, 2464
BstSCI CCNGG 4 cut(s) 643, 795, 885, 2221
BstSFI CTRYAG 2 cut(s) 825, 839
BstSLI GKGCMC 2 cut(s) 477, 796
BstV1I GCAGC 6 cut(s) 220, 369, 825, 853, 1067, 1183
BstV2I GAAGAC 5 cut(s) 715, 1407, 1808, 2160, 2304
BstX2I RGATCY 1 cut(s) 1093
BstXI CCANNNNNNTGG 1 cut(s) 1991
BstYI RGATCY 1 cut(s) 1093
Bsu15I ATCGAT 1 cut(s) 1670
Bsu36I CCTNAGG 1 cut(s) 1063
BsuI GTATCC 1 cut(s) 1938
BsuRI GGCC 1 cut(s) 1983
BsuTUI ATCGAT 1 cut(s) 1670
BtrI CACGTC 1 cut(s) 189
BtsCI GGATG 5 cut(s) 433, 656, 895, 1058, 2004
BtsIMutI CAGTG 7 cut(s) 81, 239, 665, 1356, 1500, 2122, 2348
BveI ACCTGC 1 cut(s) 1914
Cac8I GCNNGC 4 cut(s) 331, 836, 1169, 1660
CaiI CAGNNNCTG 3 cut(s) 157, 214, 394
CciI TCATGA 1 cut(s) 685
CfoI GCGC 1 cut(s) 121
Cfr13I GGNCC 3 cut(s) 148, 254, 799
ClaI ATCGAT 1 cut(s) 1670
Csp6I GTAC 2 cut(s) 1811, 2202
CviQI GTAC 2 cut(s) 1811, 2202
DdeI CTNAG 8 cut(s) 278, 546, 1063, 1532, 1773, 1839, 2114, 2192
DpnI GATC 7 cut(s) 218, 884, 907, 1095, 1159, 1341, 1673
DpnII GATC 7 cut(s) 216, 882, 905, 1093, 1157, 1339, 1671
DraIII CACNNNGTG 1 cut(s) 236
DrdI GACNNNNNNGTC 1 cut(s) 973
DriI GACNNNNNGTC 2 cut(s) 619, 1408
DseDI GACNNNNNNGTC 1 cut(s) 973
EaeI YGGCCR 1 cut(s) 1981
Eam1104I CTCTTC 3 cut(s) 1680, 1877, 2409
Eam1105I GACNNNNNGTC 2 cut(s) 619, 1408
EarI CTCTTC 3 cut(s) 1680, 1877, 2409
Ecl136II GAGCTC 1 cut(s) 1077
Eco130I CCWWGG 2 cut(s) 1321, 1984
Eco24I GRGCYC 1 cut(s) 1079
Eco32I GATATC 1 cut(s) 184
Eco47I GGWCC 3 cut(s) 148, 254, 799
Eco53kI GAGCTC 1 cut(s) 1077
Eco57I CTGAAG 7 cut(s) 371, 872, 1356, 1901, 2172, 2316, 2355
Eco81I CCTNAGG 1 cut(s) 1063
Eco88I CYCGRG 2 cut(s) 176, 2347
EcoICRI GAGCTC 1 cut(s) 1077
EcoNI CCTNNNNNAGG 1 cut(s) 218
EcoO109I RGGNCCY 1 cut(s) 799
EcoRI GAATTC 2 cut(s) 911, 1347
EcoRII CCWGG 3 cut(s) 643, 795, 885
EcoRV GATATC 1 cut(s) 184
EcoT14I CCWWGG 2 cut(s) 1321, 1984
EcoT38I GRGCYC 1 cut(s) 1079
ErhI CCWWGG 2 cut(s) 1321, 1984
FalI AAGNNNNNCTT 4 cut(s) 737, 769, 964, 996
FaqI GGGAC 1 cut(s) 785
FauI CCCGC 1 cut(s) 1160
FauNDI CATATG 1 cut(s) 1601
FbaI TGATCA 1 cut(s) 216
Fnu4HI GCNGC 6 cut(s) 209, 358, 839, 842, 1056, 1172
FokI GGATG 5 cut(s) 440, 663, 902, 1045, 1991
FriOI GRGCYC 1 cut(s) 1079
Fsp4HI GCNGC 6 cut(s) 209, 358, 839, 842, 1056, 1172
FspBI CTAG 6 cut(s) 269, 1212, 1808, 1818, 1859, 2168
GlaI GCGC 1 cut(s) 120
GluI GCNGC 6 cut(s) 209, 358, 839, 842, 1056, 1172
GsuI CTGGAG 1 cut(s) 627
HaeII RGCGCY 1 cut(s) 122
HaeIII GGCC 1 cut(s) 1983
HapII CCGG 1 cut(s) 2223
HhaI GCGC 1 cut(s) 121
Hin6I GCGC 1 cut(s) 119
HinP1I GCGC 1 cut(s) 119
HpaII CCGG 1 cut(s) 2223
HphI GGTGA 7 cut(s) 91, 118, 463, 640, 976, 1040, 2113
Hpy166II GTNNAC 2 cut(s) 192, 475
Hpy8I GTNNAC 2 cut(s) 192, 475
HpyCH4IV ACGT 2 cut(s) 188, 1117
HpyF10VI GCNNNNNNNGC 4 cut(s) 101, 1528, 1711, 2188
HpyF3I CTNAG 8 cut(s) 278, 546, 1063, 1532, 1773, 1839, 2114, 2192
HpySE526I ACGT 2 cut(s) 188, 1117
HspAI GCGC 1 cut(s) 119
Ksp22I TGATCA 1 cut(s) 216
Kzo9I GATC 7 cut(s) 216, 882, 905, 1093, 1157, 1339, 1671
LmnI GCTCC 5 cut(s) 92, 304, 646, 1074, 2508
Lsp1109I GCAGC 6 cut(s) 220, 369, 825, 853, 1067, 1183
LweI GCATC 4 cut(s) 679, 1021, 1067, 1986
MaeI CTAG 6 cut(s) 269, 1212, 1808, 1818, 1859, 2168
MaeII ACGT 2 cut(s) 188, 1117
MalI GATC 7 cut(s) 218, 884, 907, 1095, 1159, 1341, 1673
MboI GATC 7 cut(s) 216, 882, 905, 1093, 1157, 1339, 1671
MfeI CAATTG 2 cut(s) 2084, 2102
MflI RGATCY 1 cut(s) 1093
MhlI GDGCHC 4 cut(s) 477, 796, 1079, 2348
MlyI GAGTC 5 cut(s) 194, 1241, 1567, 1784, 1798
MmeI TCCRAC 4 cut(s) 378, 547, 578, 1132
MroXI GAANNNNTTC 1 cut(s) 915
MseI TTAA 5 cut(s) 135, 609, 938, 2249, 2283
MslI CAYNNNNRTG 2 cut(s) 1699, 2465
MspI CCGG 1 cut(s) 2223
MspR9I CCNGG 4 cut(s) 645, 797, 887, 2223
MunI CAATTG 2 cut(s) 2084, 2102
Mva1269I GAATGC 1 cut(s) 1313
MvaI CCWGG 3 cut(s) 645, 797, 887
MwoI GCNNNNNNNGC 4 cut(s) 101, 1528, 1711, 2188
NciI CCSGG 1 cut(s) 2223
NdeI CATATG 1 cut(s) 1601
NdeII GATC 7 cut(s) 216, 882, 905, 1093, 1157, 1339, 1671
NlaIV GGNNCC 7 cut(s) 793, 801, 1566, 2002, 2237, 2436, 2504
NmuCI GTSAC 7 cut(s) 106, 381, 964, 1462, 2090, 2119, 2401
NspI RCATGY 3 cut(s) 1531, 2409, 2464
PaeR7I CTCGAG 1 cut(s) 2347
PagI TCATGA 1 cut(s) 685
PciI ACATGT 1 cut(s) 2405
PcsI WCGNNNNNNNCGW 1 cut(s) 1415
PctI GAATGC 1 cut(s) 1313
PdmI GAANNNNTTC 1 cut(s) 915
PfeI GAWTC 7 cut(s) 128, 274, 336, 1865, 1876, 1887, 2380
PflMI CCANNNNNTGG 1 cut(s) 992
PfoI TCCNGGA 2 cut(s) 643, 885
PkrI GCNGC 6 cut(s) 210, 359, 840, 843, 1057, 1173
PleI GAGTC 5 cut(s) 194, 1240, 1566, 1783, 1798
PpsI GAGTC 5 cut(s) 194, 1240, 1566, 1783, 1798
PpuMI RGGWCCY 1 cut(s) 799
PscI ACATGT 1 cut(s) 2405
Psp124BI GAGCTC 1 cut(s) 1079
Psp1406I AACGTT 1 cut(s) 1117
Psp5II RGGWCCY 1 cut(s) 799
Psp6I CCWGG 3 cut(s) 643, 795, 885
PspGI CCWGG 3 cut(s) 643, 795, 885
PspN4I GGNNCC 7 cut(s) 793, 801, 1566, 2002, 2237, 2436, 2504
PspPI GGNCC 3 cut(s) 148, 254, 799
PspPPI RGGWCCY 1 cut(s) 799
PsrI GAACNNNNNNTAC 2 cut(s) 2427, 2459
PstI CTGCAG 2 cut(s) 829, 843
PstNI CAGNNNCTG 3 cut(s) 157, 214, 394
PsuI RGATCY 1 cut(s) 1093
RsaI GTAC 2 cut(s) 1812, 2203
RsaNI GTAC 2 cut(s) 1811, 2202
RseI CAYNNNNRTG 2 cut(s) 1699, 2465
SacI GAGCTC 1 cut(s) 1079
SaqAI TTAA 5 cut(s) 135, 609, 938, 2249, 2283
SatI GCNGC 6 cut(s) 209, 358, 839, 842, 1056, 1172
Sau3AI GATC 7 cut(s) 216, 882, 905, 1093, 1157, 1339, 1671
Sau96I GGNCC 3 cut(s) 148, 254, 799
ScaI AGTACT 1 cut(s) 1812
SchI GAGTC 5 cut(s) 194, 1241, 1567, 1784, 1798
ScrFI CCNGG 4 cut(s) 645, 797, 887, 2223
SduI GDGCHC 4 cut(s) 477, 796, 1079, 2348
SfaNI GCATC 4 cut(s) 679, 1021, 1067, 1986
SfcI CTRYAG 2 cut(s) 825, 839
Sfr274I CTCGAG 1 cut(s) 2347
SinI GGWCC 3 cut(s) 148, 254, 799
SlaI CTCGAG 1 cut(s) 2347
SmiMI CAYNNNNRTG 2 cut(s) 1699, 2465
SmlI CTYRAG 5 cut(s) 920, 1229, 2240, 2347, 2453
SmoI CTYRAG 5 cut(s) 920, 1229, 2240, 2347, 2453
SpeI ACTAGT 2 cut(s) 1817, 1858
SsiI CCGC 4 cut(s) 90, 104, 1024, 1167
SspI AATATT 1 cut(s) 2047
SspMI CTAG 6 cut(s) 269, 1212, 1808, 1818, 1859, 2168
SstI GAGCTC 1 cut(s) 1079
StyD4I CCNGG 4 cut(s) 643, 795, 885, 2221
StyI CCWWGG 2 cut(s) 1321, 1984
TaiI ACGT 2 cut(s) 191, 1120
TaqI TCGA 8 cut(s) 623, 784, 930, 1441, 1670, 1676, 2228, 2348
TatI WGTACW 2 cut(s) 1810, 2201
TfiI GAWTC 7 cut(s) 128, 274, 336, 1865, 1876, 1887, 2380
Tru1I TTAA 5 cut(s) 135, 609, 938, 2249, 2283
Tru9I TTAA 5 cut(s) 135, 609, 938, 2249, 2283
TscAI CASTG 7 cut(s) 81, 239, 672, 1363, 1507, 2122, 2348
TseFI GTSAC 7 cut(s) 106, 381, 964, 1462, 2090, 2119, 2401
TseI GCWGC 6 cut(s) 208, 357, 838, 841, 1055, 1171
Tsp45I GTSAC 7 cut(s) 106, 381, 964, 1462, 2090, 2119, 2401
TspGWI ACGGA 1 cut(s) 630
TspRI CASTG 7 cut(s) 81, 239, 672, 1363, 1507, 2122, 2348
Van91I CCANNNNNTGG 1 cut(s) 992
VneI GTGCAC 1 cut(s) 473
VpaK11BI GGWCC 3 cut(s) 148, 254, 799
XagI CCTNNNNNAGG 1 cut(s) 218
XapI RAATTY 8 cut(s) 22, 707, 911, 1347, 1902, 2173, 2251, 2424
XbaI TCTAGA 1 cut(s) 1211
XceI RCATGY 3 cut(s) 1531, 2409, 2464
XcmI CCANNNNNNNNNTGG 1 cut(s) 1182
XhoI CTCGAG 1 cut(s) 2347
XmnI GAANNNNTTC 1 cut(s) 915
XspI CTAG 6 cut(s) 269, 1212, 1808, 1818, 1859, 2168
ZrmI AGTACT 1 cut(s) 1812
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.