MD02G1165600.v1.1

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000039
Physical Location & Seq
Forward (+)
14208039 .. 14218546
10508 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1165600.v1.1.491

Sequence Viewer

Length: 2295 bp
ATGTCTGTGGCTTCAGTCGGCGTGGGTGCCCTGAGAACGGCATTTCAAATGCTGTTTGATGCGGTAAAATCAGTGCAGGAGGAGACTACTATGTTCAAGCACCTCCTCGGAGACATCAAATCCACACTGAACTCTCTGCAGCCACTCATCCAAGAGATTGAAAAATATAACTCGAAAGAGGGACTGGAATATTTTACAGTACAAATGGAGAAGGGAGCAGATCTTGTTCAAAAGTGCAGCAAAGTTGGCGTGTGGAAAAAATGCAAATACACCAAGAAGCTCCATCAACTGGAAAAATCTCTTCGAAGAGTGTTTGATGAACTCAAACTGCAGGGCGTAAGGGATGCGAGGGAGACGTTGGTTTCTCTCAGGAATATCGAGACGGCGTTCTCTCGAATAGAAAAGAATTTGGTGATGAAAAGTAATCAACCTGAAACGGTTAAATGTTCGTATGCGGTGCCTGAACCCCCATCCGTTACAGTCGGGTTGGATTTGCCGTTGAGGGAGTTGAAGATGAAGCTCCTTAAGGATGAAAAGGTTTCGATGCTTGTGCTTACAGCTGCTGGAGGATGTGGGAAAACAACCTTGGCTAAAAAGTTTTGTCAAGATCAAGAAGTCAAAGATACATTCAAGAACAATATCTTCTTTGTCAATGTGTCGAAAAAGCCCAACTTGGACCTTATTGTACATGAACTATATCAACTGAAGGGGTTTGAGCCGCCTGTTTTCCAAAACGAATTCATGGCAGTTAACTGGTTGCAACAATTTTTGAAGAAAGCAAGACACAATCCTCTGTTGTTTGTCCTGGATGACGTTTGGTCAGGATCAGAGTCCCTTCTTGATAAGTTTGATCAATTCAAGTTTTCAAATTACAAAATTTTGGTCACATCAAGATTTGCATTTCCGAGATTTGGTTCTCCATACCATTTGGCATTATTGAATGATGAGGATGCAATGGCTCTTTTTCACCATTCAGCATCCTCAGAGGATAAGAGCTCTTATACTTCCGAAGATCTTTCAAGAAAGATTATGAAGCGCTGTAAGGGATGGCCACTTGCCATTGCATTGGTCGGGAGGTCACTTCGCGGCCAGCCTTTAGAAATCTGGCAGAAAAGAGTACTGGAATGGAGCAAAGGTTCATCTATTCTTGATACTGACAAGGATTTGCTTGCTTGCCTCCAGAGCAGCTTAGATGCCTTGGATAAAGAAAAGACTATGATCATGGAATGTTTCCTCGATCTAGGTTCATTTCCCGAGGACCAAAGAATCGCTGCTGCCACCCTCATTGACATGTGGGCGGAGTTATATGGTCTAGAAGAAGAAATTCTGACCATTGCCAACCTTCATGGGCTCACCACCCGAAGTTTGGCAAACCTTGTTGTCACAAGGCGTGAGAGGGAGGCGGACGGCTATTACACCGAAGACTTTGTCACCCAGCATCACATGCTTAGAGAGTTGGCGATCCATCAGGCGATTCAAGACCCAATAGAACAGAGGAAAAGGCTAGTTATAAACATATGTGGGGACAACCTTCCTAAGTGGTTGACAGAACAAAAGTATCAAGCGTCCAAGGCTCGACTATTATCTATCTCAACAGATGGAGCCTTCTCAGCAAAATGGCCAAACATGCAAGTACCTGAAGCAGAGGTTCTAGTTTTGAATTTTCAAACAAAGAACTATGCCTTACCTCAGTTCGTGGAGAAAATGGATAAACTAAAGGTTCTAATAATCACAAATTACGGTTTGTCACCTGCTGGACTAGATAATTTTGAACTACTTGGTTCATTATCAAATCTTAAGAGAATTAGATTAGAACGTATTTCAATTCCCACCATAAGCAAGGTCCTCAAACCGTTGAAAAGTTTGCAAAAGATATCATTTTTCATGTGTGATATCGGTCAAGGTTTCAACAACTGTTCCATTTCAATCACATCAGCATTCCCGAATCTTGAAGAAATGAACATCGACTTTTGCAGTGATTTAATTGAATTGCCTTCCAAGCTTTGTGATCTTAGTAAACTAAAGAAGCTCAGTATTACTAACTGTCATAAGCTATCTGCCTTGCCTGAAGGGATTGGAAAGTTGGAAAATTTAGAAATGTTGAGGCTAAGATCCTGCACAGAACTGTCGAAGCTTCCAATTTCGATGAAGAACCTTGGAAAGTTAGAGGAAGTGGTCTGTGATGAACAGACAAAATGTTTATGGGAATCTTTCTTGTCGTGTCTCAACAACGTAAGGATAATTGTGGCGAAAGAGAATATCAACCCAAATTGGCTCCAAAAGACTCAGTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

765

Amino Acids

86.99

Weight (kDa)

8.43

Isoelectric Point (pI)

43.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 9 - 129 9.6e-23 Arabidopsis broad-spectrum mildew resistance protein RPW8
NB-ARC PF00931 167 - 327 1.9e-13 NB-ARC domain
LRR_14 PF23598 645 - 750 7.9e-09 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000039)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66890 AT5G66900 AT5G66900 AT5G66900 AT5G66910
fragaria_vesca FvH4_1g15090 FvH4_1g15120 FvH4_1g15130 FvH4_1g15140 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15170 FvH4_1g15171 FvH4_1g15171 FvH4_1g15200 FvH4_1g15200 FvH4_1g15200 FvH4_1g15210 FvH4_1g15210 FvH4_1g15210 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15230 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15500 FvH4_1g15500 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_2g19760 FvH4_3g12820 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_6g18970 FvH4_6g42370 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g50100 FvH4_6g50100 FvH4_6g50110 FvH4_6g50130 FvH4_6g50140 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50330
malus_domestica MD00G1053700.v1.1 MD02G1164300.v1.1 MD02G1164400.v1.1 MD02G1164700.v1.1 MD02G1164800.v1.1 MD02G1165300.v1.1 MD02G1165400.v1.1 MD02G1165500.v1.1 MD02G1165600.v1.1 MD02G1165900.v1.1 MD02G1166000.v1.1 MD02G1166100.v1.1 MD02G1166300.v1.1 MD08G1084900.v1.1 MD15G1263100.v1.1 MD15G1263300.v1.1 MD15G1276900.v1.1 MD15G1277200.v1.1 MD15G1277400.v1.1 MD15G1277700.v1.1 MD15G1277800.v1.1 MD15G1278000.v1.1 MD15G1278100.v1.1 MD15G1278400.v1.1 MD15G1278600.v1.1
prunus_persica Prupe.7G138500_v2.0.a1 Prupe.7G138500_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138700_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138900_v2.0.a1 Prupe.7G139100_v2.0.a1 Prupe.7G139200_v2.0.a1 Prupe.7G139500_v2.0.a1 Prupe.7G139600_v2.0.a1 Prupe.7G139700_v2.0.a1 Prupe.7G139800_v2.0.a1
pyrus_communis pycom02g13150 pycom02g13160 pycom02g13190 pycom02g13200 pycom02g13220 pycom02g13230 pycom02g13250 pycom02g13260 pycom02g13270 pycom02g13300 pycom02g13310 pycom02g13330 pycom15g23980 pycom15g23990 pycom15g24030 pycom15g24040 pycom15g24050 pycom15g24070 pycom15g24090 pycom15g24160
rosa_chinensis RchiOBHm_Chr1g0326431 RchiOBHm_Chr1g0326451 RchiOBHm_Chr2g0104121 RchiOBHm_Chr2g0104151 RchiOBHm_Chr2g0104171 RchiOBHm_Chr2g0104181 RchiOBHm_Chr2g0104191 RchiOBHm_Chr2g0104201 RchiOBHm_Chr2g0104211 RchiOBHm_Chr2g0104241 RchiOBHm_Chr2g0104261 RchiOBHm_Chr2g0104281 RchiOBHm_Chr2g0104311 RchiOBHm_Chr2g0104331 RchiOBHm_Chr2g0104361 RchiOBHm_Chr2g0104381 RchiOBHm_Chr2g0104391 RchiOBHm_Chr2g0104431 RchiOBHm_Chr2g0104441 RchiOBHm_Chr2g0104541 RchiOBHm_Chr2g0104611 RchiOBHm_Chr2g0104741 RchiOBHm_Chr2g0104761 RchiOBHm_Chr2g0158291 RchiOBHm_Chr2g0170371 RchiOBHm_Chr2g0170631 RchiOBHm_Chr3g0473971 RchiOBHm_Chr3g0480491 RchiOBHm_Chr3g0480541 RchiOBHm_Chr3g0480561 RchiOBHm_Chr3g0485281 RchiOBHm_Chr3g0485291 RchiOBHm_Chr4g0411541 RchiOBHm_Chr6g0285471
rosa_laevigata RLG00000008347 RLG00000012637 RLG00000016961 RLG00000017293 RLG00000017305 RLG00000017308 RLG00000017312 RLG00000017314 RLG00000017317 RLG00000017318 RLG00000017319 RLG00000017321 RLG00000017322 RLG00000017323 RLG00000017324 RLG00000017325 RLG00000017327 RLG00000017328 RLG00000017329 RLG00000017330 RLG00000017334 RLG00000020833 RLG00000021073 RLG00000021950 RLG00000021971 RLG00000023154 RLG00000023157 RLG00000023467 RLG00000023468 RLG00000023525 RLG00000023963 RLG00000033265 RLG00000033267 RLG00000033268
rosa_multiflora Rmu_co8053082.1_g000001 Rmu_co8210358.1_g000001 Rmu_co8331661.1_g000001 Rmu_co8477427.1_g000002 Rmu_co8483217.1_g000001 Rmu_sc0000284.1_g000004 Rmu_sc0000676.1_g000017 Rmu_sc0000676.1_g000019 Rmu_sc0000676.1_g000021 Rmu_sc0002205.1_g000038 Rmu_sc0002205.1_g000041 Rmu_sc0002316.1_g000010 Rmu_sc0002316.1_g000012 Rmu_sc0002316.1_g000040 Rmu_sc0002316.1_g000071 Rmu_sc0002316.1_g000072 Rmu_sc0002316.1_g000095 Rmu_sc0002316.1_g000102 Rmu_sc0002414.1_g000018 Rmu_sc0002576.1_g000002 Rmu_sc0002576.1_g000003 Rmu_sc0002687.1_g000010 Rmu_sc0002687.1_g000017 Rmu_sc0003660.1_g000001 Rmu_sc0003787.1_g000014 Rmu_sc0003787.1_g000020 Rmu_sc0003787.1_g000024 Rmu_sc0003787.1_g000029 Rmu_sc0004059.1_g000026 Rmu_sc0005137.1_g000021 Rmu_sc0006550.1_g000001 Rmu_sc0006561.1_g000025 Rmu_sc0006561.1_g000033 Rmu_sc0007704.1_g000005 Rmu_sc0008904.1_g000005 Rmu_sc0010270.1_g000002 Rmu_sc0010307.1_g000001 Rmu_sc0011601.1_g000013 Rmu_sc0012694.1_g000005 Rmu_sc0013101.1_g000008 Rmu_sc0019029.1_g000001 Rmu_sc0019728.1_g000001 Rmu_sc0025829.1_g000001 Rmu_sc0025829.1_g000002 Rmu_ssc0000257.1_g000007 Rmu_ssc0000257.1_g000008
rosa_roxburghii Rroxscaffold_1G00021710 Rroxscaffold_1G00049350 Rroxscaffold_1G00049360 Rroxscaffold_2G00081160 Rroxscaffold_2G00081360 Rroxscaffold_2G00081390 Rroxscaffold_2G00091540 Rroxscaffold_2G00138790 Rroxscaffold_2G00138800 Rroxscaffold_2G00138920 Rroxscaffold_2G00139000 Rroxscaffold_2G00139010 Rroxscaffold_2G00139020 Rroxscaffold_2G00139030 Rroxscaffold_2G00139040 Rroxscaffold_2G00139050 Rroxscaffold_2G00139060 Rroxscaffold_2G00139070 Rroxscaffold_2G00139090 Rroxscaffold_2G00139100 Rroxscaffold_3G00257470 Rroxscaffold_4G00318040 Rroxscaffold_4G00323740 Rroxscaffold_4G00323750 Rroxscaffold_5G00356140 Rroxscaffold_6G00401280 Rroxscaffold_6G00401290 Rroxscaffold_7G00183120
rosa_rugosa Rorug01G0060500 Rorug01G0060900 Rorug02G0119500 Rorug02G0119600 Rorug02G0119700 Rorug02G0119700 Rorug02G0119700 Rorug02G0119800.1 Rorug02G0119900.1 Rorug02G0120000 Rorug02G0120100 Rorug02G0120200 Rorug02G0120300 Rorug02G0120400 Rorug02G0120500 Rorug02G0120700 Rorug02G0120700 Rorug02G0122300 Rorug02G0122300 Rorug02G0122400.1 Rorug02G0466700 Rorug02G0466700 Rorug02G0466800 Rorug02G0548600 Rorug02G0549900 Rorug02G0550000 Rorug03G0184200 Rorug04G0107900 Rorug05G0121500 Rorug06G0174800
rosa_samantha Rh1AG198400 Rh1CG074700 Rh1CG075000 Rh1CG183700 Rh2AG169700 Rh2AG169800 Rh2AG169900 Rh2AG170000 Rh2AG170300 Rh2AG170400 Rh2AG171000 Rh2AG171100 Rh2AG171600 Rh2AG172400 Rh2AG172500 Rh2AG532900 Rh2AG620000 Rh2AG622500 Rh2AG622800 Rh2AG623000 Rh2BG176200 Rh2BG176300 Rh2BG176400 Rh2BG176500 Rh2BG176600 Rh2BG176900 Rh2BG177000 Rh2BG177100 Rh2BG177200 Rh2BG177300 Rh2BG177600 Rh2BG177700 Rh2BG177900 Rh2BG178000 Rh2BG178200 Rh2BG178300 Rh2BG178500 Rh2BG179000 Rh2BG180000 Rh2BG180100 Rh2BG259200 Rh2BG546700 Rh2BG630800 Rh2BG633500 Rh2CG176500 Rh2CG177500 Rh2CG177700 Rh2DG174400 Rh2DG174600 Rh2DG174900 Rh2DG175100 Rh2DG175200 Rh2DG175300 Rh2DG175600 Rh2DG175700 Rh2DG175800 Rh2DG175900 Rh2DG176000 Rh2DG176300 Rh2DG176400 Rh2DG176500 Rh2DG176600 Rh2DG176900 Rh2DG177100 Rh2DG177200 Rh2DG177800 Rh2DG177900 Rh2DG178700 Rh2DG178800 Rh2DG555700 Rh2DG643700 Rh2DG646300 Rh3AG235600 Rh3AG235900 Rh3AG236200 Rh3AG266700 Rh3AG266900 Rh3BG216200 Rh3BG269100 Rh3CG212700 Rh3CG265800 Rh4AG168900 Rh4BG165500 Rh4CG180300 Rh5BG261500 Rh6AG286300 Rh6BG287500 Rh6CG288600 Rh7AG022600
rosa_wichuraiana Rw0G008240 Rw0G008250 Rw0G013190 Rw0G013200 Rw2G013230 Rw2G013240 Rw2G013270 Rw2G013280 Rw2G013290 Rw2G013300 Rw2G013330 Rw2G013340 Rw2G013350 Rw2G013360 Rw2G013370 Rw2G013380 Rw2G013410 Rw2G013570 Rw2G044120 Rw2G051370 Rw2G051590 Rw3G017160 Rw3G021150 Rw3G021160 Rw3G023730 Rw3G023740 Rw4G014030 Rw6G024590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1511
AarI CACCTGC 1 cut(s) 1759
Acc36I ACCTGC 1 cut(s) 1759
AccB1I GGYRCC 2 cut(s) 26, 457
AccB7I CCANNNNNTGG 1 cut(s) 289
AccII CGCG 1 cut(s) 1086
AciI CCGC 6 cut(s) 62, 455, 719, 1086, 1298, 1403
AclWI GGATC 3 cut(s) 832, 1456, 2106
AcoI YGGCCR 3 cut(s) 1049, 1087, 1619
AcsI RAATTY 6 cut(s) 406, 737, 876, 1323, 1660, 2089
AcuI CTGAAG 3 cut(s) 725, 1659, 2088
AfaI GTAC 4 cut(s) 201, 687, 1119, 1635
AfeI AGCGCT 1 cut(s) 1037
AfiI CCNNNNNNNGG 4 cut(s) 37, 289, 911, 1366
AflII CTTAAG 2 cut(s) 524, 1796
AflIII ACRYGT 1 cut(s) 1290
AhdI GACNNNNNGTC 1 cut(s) 817
AjnI CCWGG 1 cut(s) 804
AjuI GAANNNNNNNTTGG 2 cut(s) 569, 601
AloI GAACNNNNNNTCC 2 cut(s) 1120, 1152
AluBI AGCT 9 cut(s) 280, 520, 560, 996, 1188, 2002, 2029, 2053, 2134
AluI AGCT 9 cut(s) 280, 520, 560, 996, 1188, 2002, 2029, 2053, 2134
Alw21I GWGCWC 1 cut(s) 998
Alw26I GTCTC 5 cut(s) 77, 105, 347, 374, 2228
AlwI GGATC 3 cut(s) 832, 1456, 2106
AlwNI CAGNNNCTG 1 cut(s) 563
Ama87I CYCGRG 1 cut(s) 1253
Aor51HI AGCGCT 1 cut(s) 1037
AoxI GGCC 3 cut(s) 1049, 1087, 1619
ApeKI GCWGC 6 cut(s) 139, 237, 560, 1185, 1271, 1274
ApoI RAATTY 6 cut(s) 406, 737, 876, 1323, 1660, 2089
ArsI GACNNNNNNTTYG 4 cut(s) 867, 899, 1413, 1445
Asp700I GAANNNNTTC 1 cut(s) 1323
AspLEI GCGC 1 cut(s) 1038
AspS9I GGNCC 3 cut(s) 676, 1258, 1843
AsuHPI GGTGA 5 cut(s) 424, 959, 1345, 1423, 1740
AsuII TTCGAA 1 cut(s) 304
AvaI CYCGRG 1 cut(s) 1253
AvaII GGWCC 3 cut(s) 676, 1258, 1843
BaeGI GKGCMC 1 cut(s) 31
BaeI ACNNNNGTAYC 2 cut(s) 1541, 1574
BalI TGGCCA 2 cut(s) 1051, 1621
BanI GGYRCC 2 cut(s) 26, 457
BanII GRGCYC 2 cut(s) 998, 1353
BbsI GAAGAC 1 cut(s) 1428
Bbv12I GWGCWC 1 cut(s) 998
BbvI GCAGC 6 cut(s) 151, 249, 547, 1197, 1258, 1261
BccI CCATC 5 cut(s) 291, 478, 1041, 1473, 1592
BceAI ACGGC 4 cut(s) 54, 399, 481, 1423
BcgI CGANNNNNNTGC 2 cut(s) 532, 566
BciT130I CCWGG 1 cut(s) 806
BclI TGATCA 2 cut(s) 850, 1218
BcoDI GTCTC 5 cut(s) 77, 105, 347, 374, 2228
BfaI CTAG 5 cut(s) 1241, 1313, 1505, 1652, 1760
BfmI CTRYAG 2 cut(s) 137, 329
BfoI RGCGCY 1 cut(s) 1039
BfrI CTTAAG 2 cut(s) 524, 1796
BfuAI ACCTGC 1 cut(s) 1759
BglII AGATCT 2 cut(s) 220, 1012
BisI GCNGC 8 cut(s) 140, 238, 561, 719, 1087, 1186, 1272, 1275
BlsI GCNGC 8 cut(s) 141, 239, 562, 720, 1088, 1187, 1273, 1276
BmcAI AGTACT 1 cut(s) 1119
Bme1390I CCNGG 1 cut(s) 806
Bme18I GGWCC 3 cut(s) 676, 1258, 1843
BmeRI GACNNNNNGTC 1 cut(s) 817
BmeT110I CYCGRG 1 cut(s) 1253
BmgT120I GGNCC 3 cut(s) 676, 1258, 1843
BmiI GGNNCC 4 cut(s) 28, 459, 1603, 2276
BmrFI CCNGG 1 cut(s) 806
BmsI GCATC 7 cut(s) 49, 334, 534, 940, 986, 1183, 1447
BpiI GAAGAC 1 cut(s) 1428
BpmI CTGGAG 2 cut(s) 585, 1163
Bpu14I TTCGAA 1 cut(s) 304
BsaJI CCNNGG 6 cut(s) 106, 585, 1197, 1254, 1569, 2155
BsaXI ACNNNNNCTCC 2 cut(s) 1120, 1150
Bsc4I CCNNNNNNNGG 4 cut(s) 37, 289, 911, 1366
Bse1I ACTGG 4 cut(s) 189, 294, 758, 1125
Bse3DI GCAATG 3 cut(s) 960, 1059, 1332
BseBI CCWGG 1 cut(s) 806
BseDI CCNNGG 6 cut(s) 106, 585, 1197, 1254, 1569, 2155
BseGI GGATG 9 cut(s) 147, 349, 470, 535, 575, 814, 955, 977, 1052
BseLI CCNNNNNNNGG 4 cut(s) 37, 289, 911, 1366
BseMI GCAATG 3 cut(s) 960, 1059, 1332
BseMII CTCAG 6 cut(s) 23, 382, 996, 1623, 1703, 2044
BseNI ACTGG 4 cut(s) 189, 294, 758, 1125
BseRI GAGGAG 2 cut(s) 95, 95
BseSI GKGCMC 1 cut(s) 31
BseXI GCAGC 6 cut(s) 151, 249, 547, 1197, 1258, 1261
BseYI CCCAGC 1 cut(s) 1434
BsgI GTGCAG 3 cut(s) 95, 256, 2101
Bsh1236I CGCG 1 cut(s) 1086
BshFI GGCC 3 cut(s) 1051, 1089, 1621
BshNI GGYRCC 2 cut(s) 26, 457
BsiHKAI GWGCWC 1 cut(s) 998
BsiHKCI CYCGRG 1 cut(s) 1253
BslFI GGGAC 3 cut(s) 195, 817, 1538
BslI CCNNNNNNNGG 4 cut(s) 37, 289, 911, 1366
BsmAI GTCTC 5 cut(s) 77, 105, 347, 374, 2228
BsmBI CGTCTC 2 cut(s) 347, 374
BsmFI GGGAC 3 cut(s) 195, 817, 1538
BsmI GAATGC 1 cut(s) 1937
BsnI GGCC 3 cut(s) 1051, 1089, 1621
BsoBI CYCGRG 1 cut(s) 1253
Bsp119I TTCGAA 1 cut(s) 304
Bsp1286I GDGCHC 3 cut(s) 31, 998, 1353
Bsp1407I TGTACA 1 cut(s) 685
BspACI CCGC 6 cut(s) 62, 455, 719, 1086, 1298, 1403
BspANI GGCC 3 cut(s) 1051, 1089, 1621
BspCNI CTCAG 6 cut(s) 24, 381, 995, 1622, 1702, 2043
BspFNI CGCG 1 cut(s) 1086
BspLI GGNNCC 4 cut(s) 28, 459, 1603, 2276
BspMAI CTGCAG 2 cut(s) 141, 333
BspMI ACCTGC 1 cut(s) 1759
BspPI GGATC 3 cut(s) 832, 1456, 2106
BspT104I TTCGAA 1 cut(s) 304
BspT107I GGYRCC 2 cut(s) 26, 457
BspTI CTTAAG 2 cut(s) 524, 1796
BsrDI GCAATG 3 cut(s) 960, 1059, 1332
BsrGI TGTACA 1 cut(s) 685
BsrI ACTGG 4 cut(s) 189, 294, 758, 1125
BssECI CCNNGG 6 cut(s) 106, 585, 1197, 1254, 1569, 2155
BssT1I CCWWGG 4 cut(s) 585, 1197, 1569, 2155
Bst2UI CCWGG 1 cut(s) 806
Bst4CI ACNGT 8 cut(s) 199, 439, 481, 1742, 1854, 1916, 2045, 2127
Bst6I CTCTTC 2 cut(s) 301, 306
BstAFI CTTAAG 2 cut(s) 524, 1796
BstAPI GCANNNNNTGC 1 cut(s) 1444
BstAUI TGTACA 1 cut(s) 685
BstBI TTCGAA 1 cut(s) 304
BstC8I GCNNGC 3 cut(s) 1091, 1170, 1174
BstF5I GGATG 9 cut(s) 147, 349, 470, 535, 575, 814, 955, 977, 1052
BstFNI CGCG 1 cut(s) 1086
BstH2I RGCGCY 1 cut(s) 1039
BstHHI GCGC 1 cut(s) 1038
BstMAI GTCTC 5 cut(s) 77, 105, 347, 374, 2228
BstMWI GCNNNNNNNGC 7 cut(s) 246, 1182, 1444, 1571, 1610, 1627, 1999
BstNI CCWGG 1 cut(s) 806
BstNSI RCATGY 3 cut(s) 1294, 1447, 1630
BstSCI CCNGG 1 cut(s) 804
BstSFI CTRYAG 2 cut(s) 137, 329
BstSLI GKGCMC 1 cut(s) 31
BstUI CGCG 1 cut(s) 1086
BstV1I GCAGC 6 cut(s) 151, 249, 547, 1197, 1258, 1261
BstV2I GAAGAC 1 cut(s) 1428
BstX2I RGATCY 3 cut(s) 220, 1012, 2111
BstXI CCANNNNNNTGG 1 cut(s) 1066
BstYI RGATCY 3 cut(s) 220, 1012, 2111
BsuRI GGCC 3 cut(s) 1051, 1089, 1621
BtsCI GGATG 9 cut(s) 147, 349, 470, 535, 575, 814, 955, 977, 1052
BtsI GCAGTG 1 cut(s) 1981
BtsIMutI CAGTG 3 cut(s) 78, 125, 1981
BveI ACCTGC 1 cut(s) 1759
Cac8I GCNNGC 3 cut(s) 1091, 1170, 1174
CaiI CAGNNNCTG 1 cut(s) 563
CfoI GCGC 1 cut(s) 1038
Cfr13I GGNCC 3 cut(s) 676, 1258, 1843
CseI GACGC 1 cut(s) 1554
Csp6I GTAC 4 cut(s) 200, 686, 1118, 1634
CspCI CAANNNNNGTGG 2 cut(s) 1267, 1302
CviAII CATG 8 cut(s) 689, 742, 1222, 1291, 1346, 1444, 1627, 1885
CviQI GTAC 4 cut(s) 200, 686, 1118, 1634
DriI GACNNNNNGTC 1 cut(s) 817
EaeI YGGCCR 3 cut(s) 1049, 1087, 1619
Eam1104I CTCTTC 2 cut(s) 301, 306
Eam1105I GACNNNNNGTC 1 cut(s) 817
EarI CTCTTC 2 cut(s) 301, 306
EciI GGCGGA 2 cut(s) 1313, 1418
Ecl136II GAGCTC 1 cut(s) 996
Eco130I CCWWGG 4 cut(s) 585, 1197, 1569, 2155
Eco24I GRGCYC 2 cut(s) 998, 1353
Eco32I GATATC 2 cut(s) 1875, 1894
Eco47I GGWCC 3 cut(s) 676, 1258, 1843
Eco47III AGCGCT 1 cut(s) 1037
Eco53kI GAGCTC 1 cut(s) 996
Eco57I CTGAAG 3 cut(s) 725, 1659, 2088
Eco88I CYCGRG 1 cut(s) 1253
EcoICRI GAGCTC 1 cut(s) 996
EcoO109I RGGNCCY 1 cut(s) 1843
EcoRI GAATTC 1 cut(s) 737
EcoRII CCWGG 1 cut(s) 804
EcoRV GATATC 2 cut(s) 1875, 1894
EcoT14I CCWWGG 4 cut(s) 585, 1197, 1569, 2155
EcoT38I GRGCYC 2 cut(s) 998, 1353
ErhI CCWWGG 4 cut(s) 585, 1197, 1569, 2155
Esp3I CGTCTC 2 cut(s) 347, 374
FaeI CATG 8 cut(s) 692, 745, 1225, 1294, 1349, 1447, 1630, 1888
FalI AAGNNNNNCTT 2 cut(s) 656, 688
FaqI GGGAC 3 cut(s) 195, 817, 1538
FatI CATG 8 cut(s) 688, 741, 1221, 1290, 1345, 1443, 1626, 1884
FauNDI CATATG 1 cut(s) 1517
FbaI TGATCA 2 cut(s) 850, 1218
Fnu4HI GCNGC 8 cut(s) 140, 238, 561, 719, 1087, 1186, 1272, 1275
FokI GGATG 9 cut(s) 134, 356, 457, 542, 582, 821, 962, 964, 1059
FriOI GRGCYC 2 cut(s) 998, 1353
Fsp4HI GCNGC 8 cut(s) 140, 238, 561, 719, 1087, 1186, 1272, 1275
FspBI CTAG 5 cut(s) 1241, 1313, 1505, 1652, 1760
GlaI GCGC 1 cut(s) 1037
GluI GCNGC 8 cut(s) 140, 238, 561, 719, 1087, 1186, 1272, 1275
GsaI CCCAGC 1 cut(s) 1438
GsuI CTGGAG 2 cut(s) 585, 1163
HaeII RGCGCY 1 cut(s) 1039
HaeIII GGCC 3 cut(s) 1051, 1089, 1621
HgaI GACGC 1 cut(s) 1554
HhaI GCGC 1 cut(s) 1038
Hin1II CATG 8 cut(s) 692, 745, 1225, 1294, 1349, 1447, 1630, 1888
Hin6I GCGC 1 cut(s) 1036
HinP1I GCGC 1 cut(s) 1036
HincII GTYRAC 2 cut(s) 751, 1545
HindII GTYRAC 2 cut(s) 751, 1545
HindIII AAGCTT 2 cut(s) 2000, 2132
HinfI GANTC 6 cut(s) 830, 1266, 1474, 1945, 2207, 2284
HpaI GTTAAC 1 cut(s) 751
HphI GGTGA 5 cut(s) 424, 959, 1345, 1423, 1740
Hpy166II GTNNAC 3 cut(s) 751, 1545, 2018
Hpy188I TCNGA 7 cut(s) 110, 829, 906, 985, 1009, 1329, 2294
Hpy8I GTNNAC 3 cut(s) 751, 1545, 2018
HpyAV CCTTC 8 cut(s) 205, 700, 845, 1352, 1541, 1615, 2004, 2063
HpyCH4III ACNGT 8 cut(s) 199, 439, 481, 1742, 1854, 1916, 2045, 2127
HpyCH4IV ACGT 4 cut(s) 356, 813, 1816, 2232
HpyF10VI GCNNNNNNNGC 7 cut(s) 246, 1182, 1444, 1571, 1610, 1627, 1999
HpySE526I ACGT 4 cut(s) 356, 813, 1816, 2232
Hsp92II CATG 8 cut(s) 692, 745, 1225, 1294, 1349, 1447, 1630, 1888
HspAI GCGC 1 cut(s) 1036
Ksp22I TGATCA 2 cut(s) 850, 1218
KspAI GTTAAC 1 cut(s) 751
LmnI GCTCC 6 cut(s) 215, 285, 525, 1128, 1601, 2280
Lsp1109I GCAGC 6 cut(s) 151, 249, 547, 1197, 1258, 1261
LweI GCATC 7 cut(s) 49, 334, 534, 940, 986, 1183, 1447
MaeI CTAG 5 cut(s) 1241, 1313, 1505, 1652, 1760
MaeII ACGT 4 cut(s) 356, 813, 1816, 2232
MaeIII GTNAC 6 cut(s) 475, 883, 1077, 1381, 1429, 1746
MflI RGATCY 3 cut(s) 220, 1012, 2111
MhlI GDGCHC 3 cut(s) 31, 998, 1353
MlsI TGGCCA 2 cut(s) 1051, 1621
MluNI TGGCCA 2 cut(s) 1051, 1621
MlyI GAGTC 2 cut(s) 839, 2278
MmeI TCCRAC 2 cut(s) 468, 2064
Mox20I TGGCCA 2 cut(s) 1051, 1621
MroXI GAANNNNTTC 1 cut(s) 1323
MscI TGGCCA 2 cut(s) 1051, 1621
MseI TTAA 5 cut(s) 441, 525, 750, 1797, 1982
MslI CAYNNNNRTG 1 cut(s) 1289
Msp20I TGGCCA 2 cut(s) 1051, 1621
MspA1I CMGCKG 1 cut(s) 560
MspCI CTTAAG 2 cut(s) 524, 1796
MspR9I CCNGG 1 cut(s) 806
Mva1269I GAATGC 1 cut(s) 1937
MvaI CCWGG 1 cut(s) 806
MvnI CGCG 1 cut(s) 1086
MwoI GCNNNNNNNGC 7 cut(s) 246, 1182, 1444, 1571, 1610, 1627, 1999
NdeI CATATG 1 cut(s) 1517
NlaIII CATG 8 cut(s) 692, 745, 1225, 1294, 1349, 1447, 1630, 1888
NlaIV GGNNCC 4 cut(s) 28, 459, 1603, 2276
NmuCI GTSAC 5 cut(s) 883, 1077, 1381, 1429, 1746
NspI RCATGY 3 cut(s) 1294, 1447, 1630
NspV TTCGAA 1 cut(s) 304
PaqCI CACCTGC 1 cut(s) 1759
PciI ACATGT 1 cut(s) 1290
PctI GAATGC 1 cut(s) 1937
PdmI GAANNNNTTC 1 cut(s) 1323
PfeI GAWTC 4 cut(s) 1266, 1474, 1945, 2207
PflFI GACNNNGTC 1 cut(s) 1427
PflMI CCANNNNNTGG 1 cut(s) 289
PfoI TCCNGGA 1 cut(s) 804
PkrI GCNGC 8 cut(s) 141, 239, 562, 720, 1088, 1187, 1273, 1276
PleI GAGTC 2 cut(s) 838, 2278
PpsI GAGTC 2 cut(s) 838, 2278
PpuMI RGGWCCY 1 cut(s) 1843
PscI ACATGT 1 cut(s) 1290
PsiI TTATAA 1 cut(s) 1511
Psp124BI GAGCTC 1 cut(s) 998
Psp5II RGGWCCY 1 cut(s) 1843
Psp6I CCWGG 1 cut(s) 804
PspFI CCCAGC 1 cut(s) 1434
PspGI CCWGG 1 cut(s) 804
PspN4I GGNNCC 4 cut(s) 28, 459, 1603, 2276
PspPI GGNCC 3 cut(s) 676, 1258, 1843
PspPPI RGGWCCY 1 cut(s) 1843
PstI CTGCAG 2 cut(s) 141, 333
PstNI CAGNNNCTG 1 cut(s) 563
PsuI RGATCY 3 cut(s) 220, 1012, 2111
PsyI GACNNNGTC 1 cut(s) 1427
PvuII CAGCTG 1 cut(s) 560
RsaI GTAC 4 cut(s) 201, 687, 1119, 1635
RsaNI GTAC 4 cut(s) 200, 686, 1118, 1634
RseI CAYNNNNRTG 1 cut(s) 1289
SacI GAGCTC 1 cut(s) 998
SaqAI TTAA 5 cut(s) 441, 525, 750, 1797, 1982
SatI GCNGC 8 cut(s) 140, 238, 561, 719, 1087, 1186, 1272, 1275
Sau96I GGNCC 3 cut(s) 676, 1258, 1843
ScaI AGTACT 1 cut(s) 1119
SchI GAGTC 2 cut(s) 839, 2278
ScrFI CCNGG 1 cut(s) 806
SduI GDGCHC 3 cut(s) 31, 998, 1353
SfaNI GCATC 7 cut(s) 49, 334, 534, 940, 986, 1183, 1447
SfcI CTRYAG 2 cut(s) 137, 329
SfuI TTCGAA 1 cut(s) 304
SinI GGWCC 3 cut(s) 676, 1258, 1843
SmiMI CAYNNNNRTG 1 cut(s) 1289
SmlI CTYRAG 2 cut(s) 524, 1796
SmoI CTYRAG 2 cut(s) 524, 1796
SsiI CCGC 6 cut(s) 62, 455, 719, 1086, 1298, 1403
SspI AATATT 1 cut(s) 191
SspMI CTAG 5 cut(s) 1241, 1313, 1505, 1652, 1760
SstI GAGCTC 1 cut(s) 998
StyD4I CCNGG 1 cut(s) 804
StyI CCWWGG 4 cut(s) 585, 1197, 1569, 2155
TaaI ACNGT 8 cut(s) 199, 439, 481, 1742, 1854, 1916, 2045, 2127
TaiI ACGT 4 cut(s) 359, 816, 1819, 2235
TaqII GACCGA 1 cut(s) 1886
TatI WGTACW 3 cut(s) 199, 685, 1117
TauI GCSGC 2 cut(s) 721, 1089
TfiI GAWTC 4 cut(s) 1266, 1474, 1945, 2207
Tru1I TTAA 5 cut(s) 441, 525, 750, 1797, 1982
Tru9I TTAA 5 cut(s) 441, 525, 750, 1797, 1982
TscAI CASTG 3 cut(s) 78, 132, 1981
TseFI GTSAC 5 cut(s) 883, 1077, 1381, 1429, 1746
TseI GCWGC 6 cut(s) 139, 237, 560, 1185, 1271, 1274
Tsp45I GTSAC 5 cut(s) 883, 1077, 1381, 1429, 1746
TspGWI ACGGA 1 cut(s) 463
TspRI CASTG 3 cut(s) 78, 132, 1981
Tth111I GACNNNGTC 1 cut(s) 1427
Van91I CCANNNNNTGG 1 cut(s) 289
Vha464I CTTAAG 2 cut(s) 524, 1796
VpaK11BI GGWCC 3 cut(s) 676, 1258, 1843
XapI RAATTY 6 cut(s) 406, 737, 876, 1323, 1660, 2089
XbaI TCTAGA 1 cut(s) 1312
XceI RCATGY 3 cut(s) 1294, 1447, 1630
XcmI CCANNNNNNNNNTGG 1 cut(s) 1363
XmnI GAANNNNTTC 1 cut(s) 1323
XspI CTAG 5 cut(s) 1241, 1313, 1505, 1652, 1760
ZrmI AGTACT 1 cut(s) 1119
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.