Rh2BG177600

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0000039
Physical Location & Seq
Forward (+)
15757780 .. 15766605
8826 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG177600.1

Sequence Viewer

Length: 2754 bp
ATGGAACTTGGTCGCTCGAACCAGTGCACAGAAAAATTTAAAATAGAAATAGTCGAGGGCCTAGAGCTTGTCCAAAATTGCTGTAAGGTTGGTCCATTGGAAATATCCAAACAGAGGAATTACATCAGAGAACTTCGTGAGTTGGATGGTTCACTTAAGAACTGGTTAGATATACTCATGTCGGATGGAGTGAGAGATGGGAAGGAGACCTTGCAATCGATCCTTCTTGTTTCACTGGTTCAACCTTTTCTGGGACGCATTGATTTGGATTTTCGTCTTGAACCATCATCACCACAAATGGTATTAGTCAGAGGAGAAGCTTTAGAAGCATCATTTGTGGCACTGTATGATGCCATTAAGGAAGTAAATGAACATCATTTGATGTTTAAAGCCCTCCTTGGAGATATCAGATCTACACTAGATTCTATAGAACCACTGATCAAAGAGACTGCAGAGGATGACAAGGAGTTGGATTTTCCAGAGGATGAACTTCAGAATTTTGGAGTACAAATGGAAGAGGGGATTGAGCTAATTCGTAAGTGCTCTAAGGTTGGTCCATTGAGTACTTACAAAAAATATAAATACACAAACAAACTAGGTGAACTGGGAAAATCTTTGCAAAGACTGTTGGATATACTGAAAGTGCATAGCATAAGAGATGTGAAGAAAACCTTGGTTACCGTCAAGAATATTGAGACTAAGGTCCAGCAACTTGATGTGAATATTGGGATACAAAATAATTCACCTGAAAGTATTGAAGGTTCATGGGCAATACCTGAACCTCCACCACTTGTAGTTGGATTGGATGGGCCTTTGAAGGAATTGAAGATGAAGCTTTTCAGTGATGAGATATCAATGCTTGTGCTAACTGCTCCTGGAGGATGTGGGAAAACTACATTGGCAACGAAGTTTTGTCAAGATGGAGACGTCAGAGATAAATTCAAGAACAATATCTTCTTTGTAACTGTTTCCAAGAAGCCCAACTTAAACCTTATTGTAGAAGAGCTATATCAACGCAAGGGTTCCCAGGTACCCGATTTCCAACATGAAGTAATTGCACTTAATTGGCTGCACGAATTTCTAAACCAAATGGGACAAGATCCTTTACTATTGGTTTTGGATGATGTTTGGTCTGAATCTGAATTTCTTCTTGAGAAGTTTGATGAATGGAGAATGTCTAACTGCAAGATCTTGGTGACATCAAGGTTTACATTTCCAAGATTTGGTTCTCCATATACTTTGAAATCATTGAGTGATGAAGATGCATTTGCTCTTTTTAATCATTCAGCATACTTGGGAGATGTTCGCTCTCATGTTCCAGAAGACCTTGCAAGACAGGTAATAAAACATTGTAAAGGATTTCCTCTTGCAATTACAGTGGTTGGAAGATCACTTTGTGGGCAGCCTATAGAGATCTGGCAAAAAAGATTAATTGAATGGACTAAAGGTTCATCTATTCTTGATTCTGATCATGATTTGCTTGTTTGCCTCCAAAGCAGCCTTGATGCCTTGGAGAAACAAAGTATTACCATCAAGGAATGTTTCCTAGACCTTGGTTCATTTCCAGAAGACCAAAGAATACCTGCTACTACCCTCATTGATATATGGGCGGAGTCATATAAAATAGATGAAGACTGGCTTTGCATTGCAAACCTCTGTGAACTCACCAATAGAAATCTGGCTACTCTTATAATCACAAGGAAGGACCCCATGGAGGTGGATGGCTATTACAGTGAACATTTTGTTACTCTGCATGACATTATTAGAGAATTGGCTATCTATCAGTCAAGTGTGGAATCAATAGAACAGAGAAAAAGGCTGATAATAGATATGTCTGGAGACAATTTTCCCGAGTGGTGGACGGAACAGAAATGTCAACCCATCAATGCTCACCTTTTATCTATCTCGACAGATGGAGACTTCTCATCTAAATGGCTCAACATGCAACTACCACAAGCAGAGGTTCTAATTCTGAACTTTCAGTCAAAGAAGTATGCCTTACCCGAATTTGTGGAGAAAATGGACAAATTAAAGGTTCTGATAGTCACAAAATACGGTCTTTTACCTGCTGAATTGGATAACTTTCCATTACTTGGTTCATTATCAAATCTAAAGAGAATTAGATTAGAACGAATAGCAATACCTTCCATGAGCAAGACCCCTGTACAGTTGAAGAGTTTAGAAAAGATATCATTATTCATGTGCAAAGTGGGTCAAGCCTTTGACAACTGTTCCATCGAACTTTTTAATCTATTGCCAAATGTAGTGGAAATGAACATTGATTACTGCAATGATTTGGTGAAATTGCCCGCCATGCTTTGTGACCTGATTTGCCTGAAAAAGCTTAGTATCACCAATTCTCACAAGCTATCTGTCTTACCTGAAGAAATAGGAAAGTTAGTGAACTTAAAAGTGTTGAGGCTTAGGTCTTGTACTGAATTGTCGGAGCTGCCAGACTCAATTCGGAACCTCAAATTGTTAAATTTTCTGGACATTTCTGATTGCTTTAGTATGAAGGAGCTTCCTGAACATATTGGTGAAATGTGCAGCTTAGAAACGCTAAATATGAGACAATGCTCAAGACTGCAAGAGTTGCCTGCATCAGTTTTGGATCTTGAACAGTTGAAGGATGTGATTTGTGATGAAGAGACTGAAATATTATGGAACCCCTTCTTACCCATTCTCCACAACTTACATATCAGGGTAGTGAAAGAAGATATTAACCTGAATTGGCTCCACACGTTTCCATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

917

Amino Acids

104.74

Weight (kDa)

5.12

Isoelectric Point (pI)

46.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 4 - 73 7.5e-06 Arabidopsis broad-spectrum mildew resistance protein RPW8
RPW8 PF05659 102 - 236 3.6e-24 Arabidopsis broad-spectrum mildew resistance protein RPW8
NB-ARC PF00931 271 - 430 5.2e-16 NB-ARC domain
LRR_14 PF23598 772 - 834 6.3e-08 Leucine-rich repeat region
LRR_14 PF23598 823 - 886 1.1e-07 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000039)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66890 AT5G66900 AT5G66900 AT5G66900 AT5G66910
fragaria_vesca FvH4_1g15090 FvH4_1g15120 FvH4_1g15130 FvH4_1g15140 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15160 FvH4_1g15170 FvH4_1g15171 FvH4_1g15171 FvH4_1g15200 FvH4_1g15200 FvH4_1g15200 FvH4_1g15210 FvH4_1g15210 FvH4_1g15210 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15220 FvH4_1g15230 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15240 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15330 FvH4_1g15500 FvH4_1g15500 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_1g17470 FvH4_2g19760 FvH4_3g12820 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_4g31860 FvH4_6g18970 FvH4_6g42370 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g42380 FvH4_6g50100 FvH4_6g50100 FvH4_6g50110 FvH4_6g50130 FvH4_6g50140 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50310 FvH4_6g50330
malus_domestica MD00G1053700.v1.1 MD02G1164300.v1.1 MD02G1164400.v1.1 MD02G1164700.v1.1 MD02G1164800.v1.1 MD02G1165300.v1.1 MD02G1165400.v1.1 MD02G1165500.v1.1 MD02G1165600.v1.1 MD02G1165900.v1.1 MD02G1166000.v1.1 MD02G1166100.v1.1 MD02G1166300.v1.1 MD08G1084900.v1.1 MD15G1263100.v1.1 MD15G1263300.v1.1 MD15G1276900.v1.1 MD15G1277200.v1.1 MD15G1277400.v1.1 MD15G1277700.v1.1 MD15G1277800.v1.1 MD15G1278000.v1.1 MD15G1278100.v1.1 MD15G1278400.v1.1 MD15G1278600.v1.1
prunus_persica Prupe.7G138500_v2.0.a1 Prupe.7G138500_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138600_v2.0.a1 Prupe.7G138700_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138800_v2.0.a1 Prupe.7G138900_v2.0.a1 Prupe.7G139100_v2.0.a1 Prupe.7G139200_v2.0.a1 Prupe.7G139500_v2.0.a1 Prupe.7G139600_v2.0.a1 Prupe.7G139700_v2.0.a1 Prupe.7G139800_v2.0.a1
pyrus_communis pycom02g13150 pycom02g13160 pycom02g13190 pycom02g13200 pycom02g13220 pycom02g13230 pycom02g13250 pycom02g13260 pycom02g13270 pycom02g13300 pycom02g13310 pycom02g13330 pycom15g23980 pycom15g23990 pycom15g24030 pycom15g24040 pycom15g24050 pycom15g24070 pycom15g24090 pycom15g24160
rosa_chinensis RchiOBHm_Chr1g0326431 RchiOBHm_Chr1g0326451 RchiOBHm_Chr2g0104121 RchiOBHm_Chr2g0104151 RchiOBHm_Chr2g0104171 RchiOBHm_Chr2g0104181 RchiOBHm_Chr2g0104191 RchiOBHm_Chr2g0104201 RchiOBHm_Chr2g0104211 RchiOBHm_Chr2g0104241 RchiOBHm_Chr2g0104261 RchiOBHm_Chr2g0104281 RchiOBHm_Chr2g0104311 RchiOBHm_Chr2g0104331 RchiOBHm_Chr2g0104361 RchiOBHm_Chr2g0104381 RchiOBHm_Chr2g0104391 RchiOBHm_Chr2g0104431 RchiOBHm_Chr2g0104441 RchiOBHm_Chr2g0104541 RchiOBHm_Chr2g0104611 RchiOBHm_Chr2g0104741 RchiOBHm_Chr2g0104761 RchiOBHm_Chr2g0158291 RchiOBHm_Chr2g0170371 RchiOBHm_Chr2g0170631 RchiOBHm_Chr3g0473971 RchiOBHm_Chr3g0480491 RchiOBHm_Chr3g0480541 RchiOBHm_Chr3g0480561 RchiOBHm_Chr3g0485281 RchiOBHm_Chr3g0485291 RchiOBHm_Chr4g0411541 RchiOBHm_Chr6g0285471
rosa_laevigata RLG00000008347 RLG00000012637 RLG00000016961 RLG00000017293 RLG00000017305 RLG00000017308 RLG00000017312 RLG00000017314 RLG00000017317 RLG00000017318 RLG00000017319 RLG00000017321 RLG00000017322 RLG00000017323 RLG00000017324 RLG00000017325 RLG00000017327 RLG00000017328 RLG00000017329 RLG00000017330 RLG00000017334 RLG00000020833 RLG00000021073 RLG00000021950 RLG00000021971 RLG00000023154 RLG00000023157 RLG00000023467 RLG00000023468 RLG00000023525 RLG00000023963 RLG00000033265 RLG00000033267 RLG00000033268
rosa_multiflora Rmu_co8053082.1_g000001 Rmu_co8210358.1_g000001 Rmu_co8331661.1_g000001 Rmu_co8477427.1_g000002 Rmu_co8483217.1_g000001 Rmu_sc0000284.1_g000004 Rmu_sc0000676.1_g000017 Rmu_sc0000676.1_g000019 Rmu_sc0000676.1_g000021 Rmu_sc0002205.1_g000038 Rmu_sc0002205.1_g000041 Rmu_sc0002316.1_g000010 Rmu_sc0002316.1_g000012 Rmu_sc0002316.1_g000040 Rmu_sc0002316.1_g000071 Rmu_sc0002316.1_g000072 Rmu_sc0002316.1_g000095 Rmu_sc0002316.1_g000102 Rmu_sc0002414.1_g000018 Rmu_sc0002576.1_g000002 Rmu_sc0002576.1_g000003 Rmu_sc0002687.1_g000010 Rmu_sc0002687.1_g000017 Rmu_sc0003660.1_g000001 Rmu_sc0003787.1_g000014 Rmu_sc0003787.1_g000020 Rmu_sc0003787.1_g000024 Rmu_sc0003787.1_g000029 Rmu_sc0004059.1_g000026 Rmu_sc0005137.1_g000021 Rmu_sc0006550.1_g000001 Rmu_sc0006561.1_g000025 Rmu_sc0006561.1_g000033 Rmu_sc0007704.1_g000005 Rmu_sc0008904.1_g000005 Rmu_sc0010270.1_g000002 Rmu_sc0010307.1_g000001 Rmu_sc0011601.1_g000013 Rmu_sc0012694.1_g000005 Rmu_sc0013101.1_g000008 Rmu_sc0019029.1_g000001 Rmu_sc0019728.1_g000001 Rmu_sc0025829.1_g000001 Rmu_sc0025829.1_g000002 Rmu_ssc0000257.1_g000007 Rmu_ssc0000257.1_g000008
rosa_roxburghii Rroxscaffold_1G00021710 Rroxscaffold_1G00049350 Rroxscaffold_1G00049360 Rroxscaffold_2G00081160 Rroxscaffold_2G00081360 Rroxscaffold_2G00081390 Rroxscaffold_2G00091540 Rroxscaffold_2G00138790 Rroxscaffold_2G00138800 Rroxscaffold_2G00138920 Rroxscaffold_2G00139000 Rroxscaffold_2G00139010 Rroxscaffold_2G00139020 Rroxscaffold_2G00139030 Rroxscaffold_2G00139040 Rroxscaffold_2G00139050 Rroxscaffold_2G00139060 Rroxscaffold_2G00139070 Rroxscaffold_2G00139090 Rroxscaffold_2G00139100 Rroxscaffold_3G00257470 Rroxscaffold_4G00318040 Rroxscaffold_4G00323740 Rroxscaffold_4G00323750 Rroxscaffold_5G00356140 Rroxscaffold_6G00401280 Rroxscaffold_6G00401290 Rroxscaffold_7G00183120
rosa_rugosa Rorug01G0060500 Rorug01G0060900 Rorug02G0119500 Rorug02G0119600 Rorug02G0119700 Rorug02G0119700 Rorug02G0119700 Rorug02G0119800.1 Rorug02G0119900.1 Rorug02G0120000 Rorug02G0120100 Rorug02G0120200 Rorug02G0120300 Rorug02G0120400 Rorug02G0120500 Rorug02G0120700 Rorug02G0120700 Rorug02G0122300 Rorug02G0122300 Rorug02G0122400.1 Rorug02G0466700 Rorug02G0466700 Rorug02G0466800 Rorug02G0548600 Rorug02G0549900 Rorug02G0550000 Rorug03G0184200 Rorug04G0107900 Rorug05G0121500 Rorug06G0174800
rosa_samantha Rh1AG198400 Rh1CG074700 Rh1CG075000 Rh1CG183700 Rh2AG169700 Rh2AG169800 Rh2AG169900 Rh2AG170000 Rh2AG170300 Rh2AG170400 Rh2AG171000 Rh2AG171100 Rh2AG171600 Rh2AG172400 Rh2AG172500 Rh2AG532900 Rh2AG620000 Rh2AG622500 Rh2AG622800 Rh2AG623000 Rh2BG176200 Rh2BG176300 Rh2BG176400 Rh2BG176500 Rh2BG176600 Rh2BG176900 Rh2BG177000 Rh2BG177100 Rh2BG177200 Rh2BG177300 Rh2BG177600 Rh2BG177700 Rh2BG177900 Rh2BG178000 Rh2BG178200 Rh2BG178300 Rh2BG178500 Rh2BG179000 Rh2BG180000 Rh2BG180100 Rh2BG259200 Rh2BG546700 Rh2BG630800 Rh2BG633500 Rh2CG176500 Rh2CG177500 Rh2CG177700 Rh2DG174400 Rh2DG174600 Rh2DG174900 Rh2DG175100 Rh2DG175200 Rh2DG175300 Rh2DG175600 Rh2DG175700 Rh2DG175800 Rh2DG175900 Rh2DG176000 Rh2DG176300 Rh2DG176400 Rh2DG176500 Rh2DG176600 Rh2DG176900 Rh2DG177100 Rh2DG177200 Rh2DG177800 Rh2DG177900 Rh2DG178700 Rh2DG178800 Rh2DG555700 Rh2DG643700 Rh2DG646300 Rh3AG235600 Rh3AG235900 Rh3AG236200 Rh3AG266700 Rh3AG266900 Rh3BG216200 Rh3BG269100 Rh3CG212700 Rh3CG265800 Rh4AG168900 Rh4BG165500 Rh4CG180300 Rh5BG261500 Rh6AG286300 Rh6BG287500 Rh6CG288600 Rh7AG022600
rosa_wichuraiana Rw0G008240 Rw0G008250 Rw0G013190 Rw0G013200 Rw2G013230 Rw2G013240 Rw2G013270 Rw2G013280 Rw2G013290 Rw2G013300 Rw2G013330 Rw2G013340 Rw2G013350 Rw2G013360 Rw2G013370 Rw2G013380 Rw2G013410 Rw2G013570 Rw2G044120 Rw2G051370 Rw2G051590 Rw3G017160 Rw3G021150 Rw3G021160 Rw3G023730 Rw3G023740 Rw4G014030 Rw6G024590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1691
AatII GACGTC 1 cut(s) 930
Acc36I ACCTGC 2 cut(s) 1591, 2074
Acc65I GGTACC 1 cut(s) 1030
AccB1I GGYRCC 1 cut(s) 1030
AccB7I CCANNNNNTGG 2 cut(s) 1223, 2093
AciI CCGC 2 cut(s) 1610, 2310
AclWI GGATC 3 cut(s) 214, 1094, 2619
AcsI RAATTY 7 cut(s) 35, 496, 938, 1076, 1142, 2006, 2482
AcuI CTGAAG 2 cut(s) 476, 2403
AcyI GRCGYC 1 cut(s) 927
AdeI CACNNNGTG 1 cut(s) 1397
AfaI GTAC 5 cut(s) 507, 565, 1032, 2166, 2434
AfiI CCNNNNNNNGG 6 cut(s) 114, 251, 1223, 1714, 1857, 2093
AflII CTTAAG 1 cut(s) 155
AflIII ACRYGT 1 cut(s) 2739
AjnI CCWGG 2 cut(s) 874, 1026
AjuI GAANNNNNNNTTGG 6 cut(s) 656, 688, 881, 913, 1210, 1242
AloI GAACNNNNNNTCC 2 cut(s) 1432, 1464
Alw21I GWGCWC 2 cut(s) 29, 545
Alw26I GTCTC 8 cut(s) 200, 440, 689, 918, 1833, 1911, 2563, 2642
Alw44I GTGCAC 1 cut(s) 25
AlwI GGATC 3 cut(s) 214, 1094, 2619
Ama87I CYCGRG 1 cut(s) 1850
AoxI GGCC 2 cut(s) 58, 809
ApaLI GTGCAC 1 cut(s) 25
ApeKI GCWGC 5 cut(s) 1069, 1402, 1497, 2449, 2547
ApoI RAATTY 7 cut(s) 35, 496, 938, 1076, 1142, 2006, 2482
AseI ATTAAT 1 cut(s) 1430
Asp700I GAANNNNTTC 3 cut(s) 836, 1146, 2669
Asp718I GGTACC 1 cut(s) 1030
AspS9I GGNCC 6 cut(s) 58, 92, 554, 703, 809, 1705
AsuHPI GGTGA 9 cut(s) 282, 611, 735, 1207, 1657, 1883, 2311, 2344, 2549
AvaI CYCGRG 1 cut(s) 1850
AvaII GGWCC 4 cut(s) 92, 554, 703, 1705
BaeGI GKGCMC 1 cut(s) 29
BanI GGYRCC 1 cut(s) 1030
BbsI GAAGAC 3 cut(s) 1329, 1575, 1638
Bbv12I GWGCWC 2 cut(s) 29, 545
BbvI GCAGC 5 cut(s) 1056, 1414, 1509, 2436, 2559
BciT130I CCWGG 2 cut(s) 876, 1028
BciVI GTATCC 1 cut(s) 723
BclI TGATCA 2 cut(s) 438, 1468
BcoDI GTCTC 8 cut(s) 200, 440, 689, 918, 1833, 1911, 2563, 2642
BfaI CTAG 4 cut(s) 62, 419, 596, 1547
BfmI CTRYAG 3 cut(s) 426, 450, 1407
BfrI CTTAAG 1 cut(s) 155
BfuAI ACCTGC 2 cut(s) 1591, 2074
BfuI GTATCC 1 cut(s) 723
BglII AGATCT 3 cut(s) 410, 1188, 1413
BisI GCNGC 5 cut(s) 1070, 1403, 1498, 2450, 2548
BlsI GCNGC 5 cut(s) 1071, 1404, 1499, 2451, 2549
BmcAI AGTACT 1 cut(s) 565
Bme1390I CCNGG 2 cut(s) 876, 1028
Bme18I GGWCC 4 cut(s) 92, 554, 703, 1705
BmeT110I CYCGRG 1 cut(s) 1850
BmgT120I GGNCC 6 cut(s) 58, 92, 554, 703, 809, 1705
BmiI GGNNCC 6 cut(s) 1024, 1032, 1707, 2468, 2666, 2735
BmrFI CCNGG 2 cut(s) 876, 1028
BmrI ACTGGG 1 cut(s) 614
BmsI GCATC 5 cut(s) 338, 340, 1252, 1495, 2609
BmuI ACTGGG 1 cut(s) 614
BoxI GACNNNNGTC 1 cut(s) 701
BpiI GAAGAC 3 cut(s) 1329, 1575, 1638
BpmI CTGGAG 2 cut(s) 897, 1857
Bpu10I CCTNAGC 1 cut(s) 2423
BpuEI CTTGAG 2 cut(s) 1172, 2563
Bsa29I ATCGAT 1 cut(s) 218
BsaBI GATNNNNATC 1 cut(s) 1467
BsaHI GRCGYC 1 cut(s) 927
BsaI GGTCTC 1 cut(s) 200
BsaJI CCNNGG 6 cut(s) 397, 672, 1026, 1509, 1552, 1710
BsaXI ACNNNNNCTCC 2 cut(s) 2667, 2697
Bsc4I CCNNNNNNNGG 6 cut(s) 114, 251, 1223, 1714, 1857, 2093
Bse1I ACTGG 5 cut(s) 22, 167, 240, 609, 1640
Bse3DI GCAATG 2 cut(s) 1644, 2296
Bse8I GATNNNNATC 1 cut(s) 1467
BseBI CCWGG 2 cut(s) 876, 1028
BseCI ATCGAT 1 cut(s) 218
BseDI CCNNGG 6 cut(s) 397, 672, 1026, 1509, 1552, 1710
BseGI GGATG 9 cut(s) 151, 190, 463, 490, 811, 887, 1126, 1726, 2635
BseJI GATNNNNATC 1 cut(s) 1467
BseLI CCNNNNNNNGG 6 cut(s) 114, 251, 1223, 1714, 1857, 2093
BseMI GCAATG 2 cut(s) 1644, 2296
BseNI ACTGG 5 cut(s) 22, 167, 240, 609, 1640
BseRI GAGGAG 1 cut(s) 327
BseSI GKGCMC 1 cut(s) 29
BseXI GCAGC 5 cut(s) 1056, 1414, 1509, 2436, 2559
BsgI GTGCAG 2 cut(s) 1055, 2566
BshFI GGCC 2 cut(s) 60, 811
BshNI GGYRCC 1 cut(s) 1030
BshVI ATCGAT 1 cut(s) 218
BsiHKAI GWGCWC 2 cut(s) 29, 545
BsiHKCI CYCGRG 1 cut(s) 1850
BslFI GGGAC 2 cut(s) 267, 1107
BslI CCNNNNNNNGG 6 cut(s) 114, 251, 1223, 1714, 1857, 2093
BsmAI GTCTC 8 cut(s) 200, 440, 689, 918, 1833, 1911, 2563, 2642
BsmBI CGTCTC 1 cut(s) 918
BsmFI GGGAC 2 cut(s) 267, 1107
BsnI GGCC 2 cut(s) 60, 811
Bso31I GGTCTC 1 cut(s) 200
BsoBI CYCGRG 1 cut(s) 1850
Bsp1286I GDGCHC 2 cut(s) 29, 545
Bsp1407I TGTACA 1 cut(s) 2164
Bsp143I GATC 9 cut(s) 219, 410, 438, 1099, 1188, 1388, 1413, 1468, 2611
Bsp19I CCATGG 1 cut(s) 1710
BspACI CCGC 2 cut(s) 1610, 2310
BspANI GGCC 2 cut(s) 60, 811
BspDI ATCGAT 1 cut(s) 218
BspHI TCATGA 1 cut(s) 1471
BspLI GGNNCC 6 cut(s) 1024, 1032, 1707, 2468, 2666, 2735
BspMAI CTGCAG 1 cut(s) 454
BspMI ACCTGC 2 cut(s) 1591, 2074
BspPI GGATC 3 cut(s) 214, 1094, 2619
BspQI GCTCTTC 1 cut(s) 996
BspT107I GGYRCC 1 cut(s) 1030
BspTI CTTAAG 1 cut(s) 155
BspTNI GGTCTC 1 cut(s) 200
BsrDI GCAATG 2 cut(s) 1644, 2296
BsrGI TGTACA 1 cut(s) 2164
BsrI ACTGG 5 cut(s) 22, 167, 240, 609, 1640
BssECI CCNNGG 6 cut(s) 397, 672, 1026, 1509, 1552, 1710
BssMI GATC 9 cut(s) 219, 410, 438, 1099, 1188, 1388, 1413, 1468, 2611
BssNI GRCGYC 1 cut(s) 927
BssT1I CCWWGG 5 cut(s) 397, 672, 1509, 1552, 1710
Bst2UI CCWGG 2 cut(s) 876, 1028
Bst6I CTCTTC 4 cut(s) 510, 996, 2168, 2640
BstACI GRCGYC 1 cut(s) 927
BstAFI CTTAAG 1 cut(s) 155
BstAPI GCANNNNNTGC 1 cut(s) 2593
BstAUI TGTACA 1 cut(s) 2164
BstC8I GCNNGC 2 cut(s) 2310, 2598
BstDEI CTNAG 5 cut(s) 546, 699, 2345, 2423, 2551
BstDSI CCRYGG 1 cut(s) 1710
BstEII GGTNACC 1 cut(s) 676
BstF5I GGATG 9 cut(s) 151, 190, 463, 490, 811, 887, 1126, 1726, 2635
BstKTI GATC 9 cut(s) 222, 413, 441, 1102, 1191, 1391, 1416, 1471, 2614
BstMAI GTCTC 8 cut(s) 200, 440, 689, 918, 1833, 1911, 2563, 2642
BstMBI GATC 9 cut(s) 219, 410, 438, 1099, 1188, 1388, 1413, 1468, 2611
BstMWI GCNNNNNNNGC 5 cut(s) 326, 1494, 1942, 2314, 2593
BstNI CCWGG 2 cut(s) 876, 1028
BstNSI RCATGY 1 cut(s) 1945
BstPAI GACNNNNGTC 1 cut(s) 701
BstPI GGTNACC 1 cut(s) 676
BstSCI CCNGG 2 cut(s) 874, 1026
BstSFI CTRYAG 3 cut(s) 426, 450, 1407
BstSLI GKGCMC 1 cut(s) 29
BstV1I GCAGC 5 cut(s) 1056, 1414, 1509, 2436, 2559
BstV2I GAAGAC 3 cut(s) 1329, 1575, 1638
BstX2I RGATCY 5 cut(s) 410, 1099, 1188, 1413, 2611
BstXI CCANNNNNNTGG 1 cut(s) 1717
BstYI RGATCY 5 cut(s) 410, 1099, 1188, 1413, 2611
Bsu15I ATCGAT 1 cut(s) 218
BsuI GTATCC 1 cut(s) 723
BsuRI GGCC 2 cut(s) 60, 811
BsuTUI ATCGAT 1 cut(s) 218
BtgI CCRYGG 1 cut(s) 1710
BtsCI GGATG 9 cut(s) 151, 190, 463, 490, 811, 887, 1126, 1726, 2635
BtsIMutI CAGTG 7 cut(s) 29, 233, 341, 434, 847, 1383, 1738
BveI ACCTGC 2 cut(s) 1591, 2074
Cac8I GCNNGC 2 cut(s) 2310, 2598
CciI TCATGA 1 cut(s) 1471
Cfr13I GGNCC 6 cut(s) 58, 92, 554, 703, 809, 1705
ClaI ATCGAT 1 cut(s) 218
CseI GACGC 1 cut(s) 264
Csp6I GTAC 5 cut(s) 506, 564, 1031, 2165, 2433
CspCI CAANNNNNGTGG 4 cut(s) 1359, 1394, 2247, 2282
CviQI GTAC 5 cut(s) 506, 564, 1031, 2165, 2433
DdeI CTNAG 5 cut(s) 546, 699, 2345, 2423, 2551
DpnI GATC 9 cut(s) 221, 412, 440, 1101, 1190, 1390, 1415, 1470, 2613
DpnII GATC 9 cut(s) 219, 410, 438, 1099, 1188, 1388, 1413, 1468, 2611
DraI TTTAAA 2 cut(s) 40, 388
DraIII CACNNNGTG 1 cut(s) 1397
Eam1104I CTCTTC 4 cut(s) 510, 996, 2168, 2640
EarI CTCTTC 4 cut(s) 510, 996, 2168, 2640
EciI GGCGGA 1 cut(s) 1625
Eco130I CCWWGG 5 cut(s) 397, 672, 1509, 1552, 1710
Eco31I GGTCTC 1 cut(s) 200
Eco32I GATATC 3 cut(s) 406, 852, 2190
Eco47I GGWCC 4 cut(s) 92, 554, 703, 1705
Eco57I CTGAAG 2 cut(s) 476, 2403
Eco88I CYCGRG 1 cut(s) 1850
Eco91I GGTNACC 1 cut(s) 676
EcoO109I RGGNCCY 2 cut(s) 58, 1705
EcoO65I GGTNACC 1 cut(s) 676
EcoRII CCWGG 2 cut(s) 874, 1026
EcoRV GATATC 3 cut(s) 406, 852, 2190
EcoT14I CCWWGG 5 cut(s) 397, 672, 1509, 1552, 1710
EcoT22I ATGCAT 1 cut(s) 1267
ErhI CCWWGG 5 cut(s) 397, 672, 1509, 1552, 1710
Esp3I CGTCTC 1 cut(s) 918
FaqI GGGAC 2 cut(s) 267, 1107
FauI CCCGC 1 cut(s) 2317
FbaI TGATCA 2 cut(s) 438, 1468
Fnu4HI GCNGC 5 cut(s) 1070, 1403, 1498, 2450, 2548
FokI GGATG 9 cut(s) 158, 197, 470, 497, 818, 894, 1133, 1733, 2642
Fsp4HI GCNGC 5 cut(s) 1070, 1403, 1498, 2450, 2548
FspBI CTAG 4 cut(s) 62, 419, 596, 1547
GluI GCNGC 5 cut(s) 1070, 1403, 1498, 2450, 2548
GsuI CTGGAG 2 cut(s) 897, 1857
HaeIII GGCC 2 cut(s) 60, 811
HgaI GACGC 1 cut(s) 264
Hin1I GRCGYC 1 cut(s) 927
HincII GTYRAC 1 cut(s) 1877
HindII GTYRAC 1 cut(s) 1877
HindIII AAGCTT 3 cut(s) 318, 833, 2342
HinfI GANTC 6 cut(s) 422, 1136, 1463, 1613, 1796, 2456
HphI GGTGA 9 cut(s) 282, 611, 735, 1207, 1657, 1883, 2311, 2344, 2549
Hpy166II GTNNAC 9 cut(s) 27, 152, 602, 1209, 1661, 1736, 1860, 1877, 2404
Hpy8I GTNNAC 9 cut(s) 27, 152, 602, 1209, 1661, 1736, 1860, 1877, 2404
HpyAV CCTTC 9 cut(s) 196, 233, 752, 811, 1696, 2154, 2509, 2620, 2680
HpyCH4IV ACGT 2 cut(s) 927, 2741
HpyF10VI GCNNNNNNNGC 5 cut(s) 326, 1494, 1942, 2314, 2593
HpyF3I CTNAG 5 cut(s) 546, 699, 2345, 2423, 2551
HpySE526I ACGT 2 cut(s) 927, 2741
Hsp92I GRCGYC 1 cut(s) 927
KpnI GGTACC 1 cut(s) 1034
Ksp22I TGATCA 2 cut(s) 438, 1468
Kzo9I GATC 9 cut(s) 219, 410, 438, 1099, 1188, 1388, 1413, 1468, 2611
LguI GCTCTTC 1 cut(s) 996
LmnI GCTCC 4 cut(s) 877, 2446, 2518, 2739
Lsp1109I GCAGC 5 cut(s) 1056, 1414, 1509, 2436, 2559
LweI GCATC 5 cut(s) 338, 340, 1252, 1495, 2609
MaeI CTAG 4 cut(s) 62, 419, 596, 1547
MaeII ACGT 2 cut(s) 927, 2741
MaeIII GTNAC 6 cut(s) 676, 961, 1195, 1744, 2044, 2321
MalI GATC 9 cut(s) 221, 412, 440, 1101, 1190, 1390, 1415, 1470, 2613
MboI GATC 9 cut(s) 219, 410, 438, 1099, 1188, 1388, 1413, 1468, 2611
MflI RGATCY 5 cut(s) 410, 1099, 1188, 1413, 2611
MhlI GDGCHC 2 cut(s) 29, 545
MlyI GAGTC 2 cut(s) 1622, 2450
MmeI TCCRAC 8 cut(s) 123, 162, 450, 609, 778, 1066, 1363, 2424
Mph1103I ATGCAT 1 cut(s) 1267
MroXI GAANNNNTTC 3 cut(s) 836, 1146, 2669
MslI CAYNNNNRTG 3 cut(s) 1715, 1930, 2535
MspCI CTTAAG 1 cut(s) 155
MspR9I CCNGG 2 cut(s) 876, 1028
MvaI CCWGG 2 cut(s) 876, 1028
MwoI GCNNNNNNNGC 5 cut(s) 326, 1494, 1942, 2314, 2593
NcoI CCATGG 1 cut(s) 1710
NdeII GATC 9 cut(s) 219, 410, 438, 1099, 1188, 1388, 1413, 1468, 2611
NlaIV GGNNCC 6 cut(s) 1024, 1032, 1707, 2468, 2666, 2735
NmuCI GTSAC 3 cut(s) 1195, 2044, 2321
NsiI ATGCAT 1 cut(s) 1267
NspI RCATGY 1 cut(s) 1945
PagI TCATGA 1 cut(s) 1471
PciSI GCTCTTC 1 cut(s) 996
PdmI GAANNNNTTC 3 cut(s) 836, 1146, 2669
PfeI GAWTC 4 cut(s) 422, 1136, 1463, 1796
PflMI CCANNNNNTGG 2 cut(s) 1223, 2093
PfoI TCCNGGA 1 cut(s) 874
PkrI GCNGC 5 cut(s) 1071, 1404, 1499, 2451, 2549
PleI GAGTC 2 cut(s) 1621, 2450
PpsI GAGTC 2 cut(s) 1621, 2450
PpuMI RGGWCCY 1 cut(s) 1705
PshAI GACNNNNGTC 1 cut(s) 701
PshBI ATTAAT 1 cut(s) 1430
PsiI TTATAA 1 cut(s) 1691
Psp5II RGGWCCY 1 cut(s) 1705
Psp6I CCWGG 2 cut(s) 874, 1026
PspEI GGTNACC 1 cut(s) 676
PspGI CCWGG 2 cut(s) 874, 1026
PspN4I GGNNCC 6 cut(s) 1024, 1032, 1707, 2468, 2666, 2735
PspPI GGNCC 6 cut(s) 58, 92, 554, 703, 809, 1705
PspPPI RGGWCCY 1 cut(s) 1705
PsrI GAACNNNNNNTAC 4 cut(s) 745, 777, 2267, 2299
PstI CTGCAG 1 cut(s) 454
PsuI RGATCY 5 cut(s) 410, 1099, 1188, 1413, 2611
RsaI GTAC 5 cut(s) 507, 565, 1032, 2166, 2434
RsaNI GTAC 5 cut(s) 506, 564, 1031, 2165, 2433
RseI CAYNNNNRTG 3 cut(s) 1715, 1930, 2535
SapI GCTCTTC 1 cut(s) 996
SatI GCNGC 5 cut(s) 1070, 1403, 1498, 2450, 2548
Sau3AI GATC 9 cut(s) 219, 410, 438, 1099, 1188, 1388, 1413, 1468, 2611
Sau96I GGNCC 6 cut(s) 58, 92, 554, 703, 809, 1705
ScaI AGTACT 1 cut(s) 565
SchI GAGTC 2 cut(s) 1622, 2450
ScrFI CCNGG 2 cut(s) 876, 1028
SduI GDGCHC 2 cut(s) 29, 545
SfaNI GCATC 5 cut(s) 338, 340, 1252, 1495, 2609
SfcI CTRYAG 3 cut(s) 426, 450, 1407
SinI GGWCC 4 cut(s) 92, 554, 703, 1705
SmiMI CAYNNNNRTG 3 cut(s) 1715, 1930, 2535
SmlI CTYRAG 3 cut(s) 155, 1151, 2578
SmoI CTYRAG 3 cut(s) 155, 1151, 2578
SsiI CCGC 2 cut(s) 1610, 2310
SspI AATATT 3 cut(s) 691, 724, 2658
SspMI CTAG 4 cut(s) 62, 419, 596, 1547
StyD4I CCNGG 2 cut(s) 874, 1026
StyI CCWWGG 5 cut(s) 397, 672, 1509, 1552, 1710
TaiI ACGT 2 cut(s) 930, 2744
TaqI TCGA 5 cut(s) 17, 54, 218, 1907, 2238
TatI WGTACW 4 cut(s) 505, 563, 2164, 2432
TfiI GAWTC 4 cut(s) 422, 1136, 1463, 1796
TscAI CASTG 7 cut(s) 29, 240, 348, 441, 847, 1383, 1738
TseFI GTSAC 3 cut(s) 1195, 2044, 2321
TseI GCWGC 5 cut(s) 1069, 1402, 1497, 2449, 2547
Tsp45I GTSAC 3 cut(s) 1195, 2044, 2321
TspGWI ACGGA 1 cut(s) 1877
TspRI CASTG 7 cut(s) 29, 240, 348, 441, 847, 1383, 1738
Van91I CCANNNNNTGG 2 cut(s) 1223, 2093
Vha464I CTTAAG 1 cut(s) 155
VneI GTGCAC 1 cut(s) 25
VpaK11BI GGWCC 4 cut(s) 92, 554, 703, 1705
VspI ATTAAT 1 cut(s) 1430
XapI RAATTY 7 cut(s) 35, 496, 938, 1076, 1142, 2006, 2482
XceI RCATGY 1 cut(s) 1945
XcmI CCANNNNNNNNNTGG 1 cut(s) 1675
XmnI GAANNNNTTC 3 cut(s) 836, 1146, 2669
XspI CTAG 4 cut(s) 62, 419, 596, 1547
ZraI GACGTC 1 cut(s) 928
ZrmI AGTACT 1 cut(s) 565
Zsp2I ATGCAT 1 cut(s) 1267
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.