FvH4_2g04610

Domain of unknown function (DUF4283)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000073
Physical Location & Seq
Forward (+)
3530817 .. 3533605
2789 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g04610.t1

Sequence Viewer

Length: 2025 bp
ATGGAAACTCACTTATTCAACCCAGGCTTGGTTAACTCATCCATCACCAACCCCACTGAACAAAGAGCTGACTCATACCCAACACCAACTACCCAAGCTTCCCTATCTACTCATCCCCCAAACACTACCCCAAGTAGCTCTATGGACAACCTCTCGGATGACACCGACCAGTTGGTTTTGCAGCTAGTCAGGAGTCTCAACCTCAACCAAGCGAGAAAGCCAGTCAAGTTGATTGGGGTCCTATTCGCAGATAAACCACCTCGAGCAATTGGTTTGAAAAAAGCCATCCAGAGCTCTTGGAAGCGGTTCGGAGCAGTGGGCGTTGAAGTAGCTGAAGATAATCTTGTGGCAATATCAGTGGACAACCCAGGAGTAGCTCAGAGCATTCTTGAAGAAAGCCCTTGGTCCATCATGGGGTATTCTCTGAAGATTGAAGAGTGGCAATCAAATATGGCAATTGAGGAAGTCAATAAAACACATCTGCCTCTATGGGTACAAGCAAAGGGTATTACTTTAGAGGAACTCACCCCAGAAAGTGCTGAGCTCATTGGAAATGAAGTGGGGAAAATTCTGGAAGCTGAAAACCCACTTACACATGAGTGTTTCAGGGGATTTCTTAGAGTAAAGGTGGAAATCGAGGCAAGTATGCCATTGAAGGATGGATTCTGGATGCCGCGAGAGGATGAAGACCAATCATGGGTGGAATTGAAATACGAGAGGTTGTCTGATTTTTGTTTTGGATGTGCAAGGCTTGGCCATACCGTGAATGCATGCATGTTCGGGGAAAGGGATGAATTTGGCACCATAATCAGGAAATTTGATTCAAGATTGAGAGCAAGGATCATTAGACCGGAGCCATTACCAGCCATGGAGATTGATGGGCTAACTCCGACTCGGAGAACAGCCACGATGCGGCGGAGTCAGTTTGCCCGGAACGGGGCACGTAGTGCCACAACAATGGTCCATGCTAATTTGGGTCCAGCAATTCACAGTGTCCAGAGGGATCAGACTGGGCACCTTGCACCCCAACAGCCAAGTGTCCAGAGGGATCAGACTGGGCACCTTACACCCCAACAGCCAAGCATGGGATCACGCCAGACCAACTCCGAAACGCATCAGACTGTAAACTGTGCCCAAGACAAACTTGTGTCAGTGGAATCAGCCCAACGGCTACTAAGAGGTAAAGACCATATCTCCACTAGTGATATTCCCTCTAGAGCCCCAAACAGGCCCACTGTCTCTACCCTCTCAGTCATTAAGGATTGGCTAACCACTAGCCCAAGTAGCCCAAACCAAAATTCACAGGTTTTTAAAGGCCCAAGTAGTTGGCAACTACATCAAGACCCAAGCAACCTAATTCTACCAAGGGCGGTAGGCCCAAATCCCCATGAGCCATGCCTAGACCCACAACTCAATGATACCCCGATCGGTAGCCCAACCTCTCAAACCCACCATAATGTCATTGGTGAGAGAGACATGCCACAACATGAACATTGTTCAAGTATCTGCTGTTTGGGAAAACGACCACTCACCTGTCATGAGAAAGCCCGTCTGTGTGGGGAGCTAGATCACTCTTCAGCCATTCCAATGGTCAATACTCTCCCAGCATTAACCAGCTACCCTCAACCAACTACTACTTCACAAATTACCAAACCTCTCACCTCACCTCATACCTCTCTGAAGAGAAACCTAACCTCAGAAACCTCACAAGCCAACCTCAAATCTAAGCTCCGAAAAGTCTCTTTACCTTCCAACTCTGGAGGTGTCGTTATTAGGGGTGCTCCCTCGCGTTCTAGAGGGGGAAGGGGCAAGGGAAGAAGAGGTCCCAACATGGGTGGTGTTTGCACTCTAGATGGGAGAAGGAACTTTGGGAGTAGTGTTGAAAGGGAGGTCTACAGCGAGATAGTAACAGTTAATGAAGGATCACAAGATATTTCAGCCTCAAGTAGTGGGGATGTTCAGATGGGGGATGTTATGTCTCCGGGCGGTGGCGGCTGGCCTACAACAGCCGCAAGGTCCCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 zf-CCHC_4 zf-RING_2

Protein Analysis

675

Amino Acids

73.71

Weight (kDa)

7.64

Isoelectric Point (pI)

54.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4283 PF14111 84 - 154 3.2e-06 Domain of unknown function (DUF4283)
zf-CCHC_4 PF14392 229 - 259 9.5e-09 Zinc knuckle
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000073)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g17821 FvH4_2g04610 FvH4_2g09611 FvH4_2g13563 FvH4_3g21493 FvH4_3g32115 FvH4_3g35060 FvH4_4g00220 FvH4_4g06410 FvH4_4g10367 FvH4_4g10367 FvH4_4g10367 FvH4_5g06101 FvH4_5g08842 FvH4_5g27366 FvH4_5g31283 FvH4_6g11611 FvH4_6g23032 FvH4_7g05661 FvH4_7g06172
malus_domestica MD13G1263700.v1.1
prunus_persica Prupe.1G292300_v2.0.a1 Prupe.5G009700_v2.0.a1 Prupe.6G158100_v2.0.a1 Prupe.8G025100_v2.0.a1 Prupe.8G042600_v2.0.a1
pyrus_communis pycom02g02250 pycom02g16030 pycom05g10490 pycom09g06450 pycom09g06460 pycom13g26200 pycom16g17700
rosa_chinensis RchiOBHm_Chr2g0163321 RchiOBHm_Chr4g0401981 RchiOBHm_Chr5g0005991 RchiOBHm_Chr5g0027191 RchiOBHm_Chr6g0249771
rosa_laevigata RLG00000001040 RLG00000001764 RLG00000002239 RLG00000002240 RLG00000003259 RLG00000008993 RLG00000013847 RLG00000014178 RLG00000015576 RLG00000017269 RLG00000017355 RLG00000018127 RLG00000018602 RLG00000018615 RLG00000019904 RLG00000020044 RLG00000021849 RLG00000021850 RLG00000027965 RLG00000029266 RLG00000029820 RLG00000031112 RLG00000031915 RLG00000033568 RLG00000035013 RLG00000035677 RLG00000036568 RLG00000036628
rosa_multiflora Rmu_co8254085.1_g000001 Rmu_co8462383.1_g000001 Rmu_sc0000185.1_g000049 Rmu_sc0000251.1_g000018 Rmu_sc0000367.1_g000094 Rmu_sc0000498.1_g000023 Rmu_sc0000868.1_g000005 Rmu_sc0001521.1_g000047 Rmu_sc0001534.1_g000007 Rmu_sc0002014.1_g000012 Rmu_sc0003214.1_g000009 Rmu_sc0003573.1_g000041 Rmu_sc0003832.1_g000023 Rmu_sc0004677.1_g000015 Rmu_sc0004967.1_g000014 Rmu_sc0005426.1_g000001 Rmu_sc0005621.1_g000004 Rmu_sc0005640.1_g000009 Rmu_sc0005947.1_g000034 Rmu_sc0006889.1_g000011 Rmu_sc0007121.1_g000014 Rmu_sc0009191.1_g000017 Rmu_sc0010556.1_g000011 Rmu_sc0011396.1_g000007 Rmu_sc0019862.1_g000002 Rmu_sc0022931.1_g000003 Rmu_sc0024164.1_g000001 Rmu_ssc0000391.1_g000027
rosa_roxburghii Rroxscaffold_1G00050060 Rroxscaffold_1G00061810 Rroxscaffold_2G00109590 Rroxscaffold_2G00113810 Rroxscaffold_2G00124040 Rroxscaffold_2G00143620 Rroxscaffold_2G00154760 Rroxscaffold_4G00302820 Rroxscaffold_4G00302830 Rroxscaffold_4G00308680 Rroxscaffold_4G00310240 Rroxscaffold_4G00327890 Rroxscaffold_5G00334600 Rroxscaffold_5G00336050 Rroxscaffold_5G00346560 Rroxscaffold_5G00347860 Rroxscaffold_5G00347870 Rroxscaffold_5G00349330 Rroxscaffold_5G00351980 Rroxscaffold_6G00395280 Rroxscaffold_6G00396750 Rroxscaffold_6G00402910 Rroxscaffold_7G00174790 Rroxscaffold_7G00177570 Rroxscaffold_7G00191820 Rroxscaffold_7G00203480 Rroxscaffold_7G00204380
rosa_rugosa Rorug01G0106600 Rorug01G0106600 Rorug01G0148500.1 Rorug02G0224200 Rorug02G0225500 Rorug02G0233000 Rorug02G0630400 Rorug03G0198000 Rorug03G0232600.1 Rorug03G0240500 Rorug04G0032900 Rorug04G0073300 Rorug04G0118500 Rorug04G0149600.1 Rorug04G0205100 Rorug04G0256700 Rorug05G0020200 Rorug05G0050000 Rorug05G0247300 Rorug05G0295100 Rorug05G0337900 Rorug05G0542600 Rorug06G0034800 Rorug06G0035100 Rorug06G0220100 Rorug07G0091900 Rorug07G0271200
rosa_samantha Rh1AG118200 Rh1AG253000 Rh1AG296300 Rh1AG409000 Rh1AG410600 Rh1BG073400 Rh1BG130400 Rh1BG147300 Rh1BG163600 Rh1CG016000 Rh1DG012200 Rh1DG012300 Rh1DG078600 Rh1DG078800 Rh1DG138100 Rh1DG179200 Rh1DG192600 Rh1DG194500 Rh1DG194600 Rh1DG214300 Rh1DG245900 Rh1DG267000 Rh1DG322700 Rh1DG442700 Rh2AG282000 Rh2AG423000 Rh2AG423100 Rh2AG460700 Rh2AG466200 Rh2AG513400 Rh2AG547000 Rh2BG277800 Rh2BG281200 Rh2BG298300 Rh2BG304300 Rh2BG433500 Rh2CG276000 Rh2CG282600 Rh2CG325500 Rh2CG393000 Rh2DG319200 Rh2DG443400 Rh3AG169300 Rh3AG303600 Rh3BG195000 Rh3BG282600 Rh3BG343400 Rh3BG343500 Rh3BG344100 Rh3CG111700 Rh3CG185700 Rh3CG259100 Rh3CG293000 Rh3CG317900 Rh3CG340900 Rh3CG341000 Rh3CG341100 Rh3CG354700 Rh3CG359200 Rh3CG368100 Rh3DG179900 Rh3DG288700 Rh3DG302600 Rh3DG316000 Rh3DG324500 Rh3DG324600 Rh3DG343200 Rh3DG343300 Rh3DG362400 Rh4AG071800 Rh4BG034000 Rh4BG044300 Rh4BG044400 Rh4BG069600 Rh4BG157400 Rh4CG007000 Rh4CG030400 Rh4CG037900 Rh4CG077700 Rh4CG115000 Rh4CG162400 Rh4CG162500 Rh4CG165500 Rh4CG165600 Rh4CG172900 Rh4CG239700 Rh4DG055900 Rh4DG173200 Rh5AG354900 Rh5AG511100 Rh5BG112600 Rh5BG541000 Rh5BG541100 Rh5BG549300 Rh5CG004400 Rh5CG315000 Rh5CG563800 Rh5CG572100 Rh5DG112200 Rh5DG191300 Rh5DG407600 Rh5DG407700 Rh5DG567500 Rh6AG002600 Rh6AG051400 Rh6AG199800 Rh6AG199900 Rh6AG206200 Rh6AG391000 Rh6AG391100 Rh6BG007500 Rh6BG053400 Rh6BG225900 Rh6BG227100 Rh6BG232400 Rh6CG091100 Rh6CG125400 Rh6CG186700 Rh6CG222700 Rh6CG222800 Rh6CG235500 Rh6CG247200 Rh6CG331200 Rh6CG465100 Rh6DG183500 Rh6DG213500 Rh6DG238800 Rh6DG263700 Rh7AG211800 Rh7AG441800 Rh7BG008600 Rh7BG253000 Rh7BG390000 Rh7CG189600 Rh7CG401500 Rh7CG424200 Rh7CG428700 Rh7CG479800 Rh7CG494300 Rh7DG177000 Rh7DG218700 Rh7DG254000 Rh7DG294800 Rh7DG407200 Rh7DG448800 Rh7DG461500
rosa_wichuraiana Rw0G012180 Rw1G005270 Rw1G012310 Rw1G016890 Rw1G021700 Rw2G014380 Rw2G027690 Rw3G016400 Rw3G028230 Rw3G029320 Rw4G002060 Rw4G002100 Rw4G003980 Rw4G014140 Rw4G014770 Rw4G014780 Rw4G015760 Rw4G021230 Rw5G000520 Rw5G035610 Rw6G001680 Rw6G017890 Rw6G027440 Rw6G034130 Rw6G034170 Rw7G012430 Rw7G033870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 800, 1014, 1059
AccI GTMKAC 1 cut(s) 1893
AccII CGCG 2 cut(s) 676, 1789
AciI CCGC 8 cut(s) 304, 674, 913, 916, 1370, 1986, 1992, 2010
AclWI GGATC 5 cut(s) 848, 1011, 1056, 1096, 1930
AcoI YGGCCR 1 cut(s) 754
AcsI RAATTY 4 cut(s) 567, 794, 815, 1297
AcuI CTGAAG 4 cut(s) 354, 446, 1560, 1700
AdeI CACNNNGTG 1 cut(s) 947
AfaI GTAC 1 cut(s) 495
AhlI ACTAGT 1 cut(s) 1199
AjnI CCWGG 2 cut(s) 22, 367
AjuI GAANNNNNNNTTGG 4 cut(s) 1282, 1314, 1621, 1653
AleI CACNNNNGTG 1 cut(s) 598
Alw21I GWGCWC 3 cut(s) 296, 546, 1783
Alw26I GTCTC 5 cut(s) 200, 1243, 1467, 1744, 1983
AlwI GGATC 5 cut(s) 848, 1011, 1056, 1096, 1930
Ama87I CYCGRG 1 cut(s) 261
AoxI GGCC 5 cut(s) 754, 1229, 1315, 1375, 1997
ApeKI GCWGC 1 cut(s) 181
ApoI RAATTY 4 cut(s) 567, 794, 815, 1297
Asp700I GAANNNNTTC 1 cut(s) 305
AspS9I GGNCC 9 cut(s) 238, 405, 961, 977, 1230, 1316, 1376, 1823, 2016
AsuC2I CCSGG 2 cut(s) 931, 1983
AsuHPI GGTGA 6 cut(s) 37, 517, 1478, 1522, 1651, 1656
AvaI CYCGRG 1 cut(s) 261
AvaII GGWCC 6 cut(s) 238, 405, 961, 977, 1823, 2016
BaeGI GKGCMC 4 cut(s) 943, 1017, 1062, 1135
BalI TGGCCA 1 cut(s) 756
BanI GGYRCC 3 cut(s) 800, 1014, 1059
BanII GRGCYC 3 cut(s) 296, 546, 1222
BbsI GAAGAC 1 cut(s) 693
Bbv12I GWGCWC 3 cut(s) 296, 546, 1783
BbvI GCAGC 1 cut(s) 193
BccI CCATC 7 cut(s) 50, 293, 416, 653, 872, 1847, 1957
BceAI ACGGC 1 cut(s) 1184
BciT130I CCWGG 2 cut(s) 24, 369
BcnI CCSGG 2 cut(s) 931, 1983
BcoDI GTCTC 5 cut(s) 200, 1243, 1467, 1744, 1983
BcuI ACTAGT 1 cut(s) 1199
BfaI CTAG 8 cut(s) 185, 1200, 1215, 1275, 1400, 1565, 1794, 1850
BfmI CTRYAG 1 cut(s) 1894
BisI GCNGC 5 cut(s) 182, 674, 914, 1993, 2010
BlpI GCTNAGC 1 cut(s) 540
BlsI GCNGC 5 cut(s) 183, 675, 915, 1994, 2011
Bme1390I CCNGG 4 cut(s) 24, 369, 931, 1983
Bme18I GGWCC 6 cut(s) 238, 405, 961, 977, 1823, 2016
BmeT110I CYCGRG 1 cut(s) 261
BmgT120I GGNCC 9 cut(s) 238, 405, 961, 977, 1230, 1316, 1376, 1823, 2016
BmiI GGNNCC 8 cut(s) 239, 802, 855, 978, 1016, 1061, 1825, 2018
BmrFI CCNGG 4 cut(s) 24, 369, 931, 1983
BmrI ACTGGG 2 cut(s) 1020, 1065
BmsI GCATC 3 cut(s) 660, 900, 1123
BmuI ACTGGG 2 cut(s) 1020, 1065
BpiI GAAGAC 1 cut(s) 693
BpmI CTGGAG 1 cut(s) 1779
Bpu1102I GCTNAGC 1 cut(s) 540
BpuEI CTTGAG 1 cut(s) 1927
BpuMI CCSGG 2 cut(s) 931, 1983
BsaAI YACGTR 1 cut(s) 944
BsaJI CCNNGG 5 cut(s) 22, 367, 401, 867, 1364
BsaWI WCCGGW 1 cut(s) 850
BsaXI ACNNNNNCTCC 4 cut(s) 1178, 1208, 1752, 1782
Bse1I ACTGG 4 cut(s) 169, 221, 1015, 1060
BseBI CCWGG 2 cut(s) 24, 369
BseDI CCNNGG 5 cut(s) 22, 367, 401, 867, 1364
BseMII CTCAG 4 cut(s) 392, 531, 1263, 1710
BseNI ACTGG 4 cut(s) 169, 221, 1015, 1060
BseSI GKGCMC 4 cut(s) 943, 1017, 1062, 1135
BseXI GCAGC 1 cut(s) 193
BseYI CCCAGC 1 cut(s) 1603
Bsh1236I CGCG 2 cut(s) 676, 1789
Bsh1285I CGRYCG 1 cut(s) 1428
BshFI GGCC 5 cut(s) 756, 1231, 1317, 1377, 1999
BshNI GGYRCC 3 cut(s) 800, 1014, 1059
BsiEI CGRYCG 1 cut(s) 1428
BsiHKAI GWGCWC 3 cut(s) 296, 546, 1783
BsiHKCI CYCGRG 1 cut(s) 261
BsiSI CCGG 3 cut(s) 851, 931, 1982
BslFI GGGAC 2 cut(s) 1809, 2002
BsmAI GTCTC 5 cut(s) 200, 1243, 1467, 1744, 1983
BsmFI GGGAC 2 cut(s) 1809, 2002
BsmI GAATGC 2 cut(s) 384, 772
BsnI GGCC 5 cut(s) 756, 1231, 1317, 1377, 1999
BsoBI CYCGRG 1 cut(s) 261
Bsp1286I GDGCHC 8 cut(s) 296, 546, 943, 1017, 1062, 1135, 1222, 1783
Bsp143I GATC 7 cut(s) 840, 1003, 1048, 1088, 1425, 1567, 1922
Bsp1720I GCTNAGC 1 cut(s) 540
Bsp19I CCATGG 1 cut(s) 867
BspACI CCGC 8 cut(s) 304, 674, 913, 916, 1370, 1986, 1992, 2010
BspANI GGCC 5 cut(s) 756, 1231, 1317, 1377, 1999
BspCNI CTCAG 4 cut(s) 391, 532, 1262, 1709
BspFNI CGCG 2 cut(s) 676, 1789
BspHI TCATGA 1 cut(s) 1537
BspLI GGNNCC 8 cut(s) 239, 802, 855, 978, 1016, 1061, 1825, 2018
BspPI GGATC 5 cut(s) 848, 1011, 1056, 1096, 1930
BspT107I GGYRCC 3 cut(s) 800, 1014, 1059
BsrI ACTGG 4 cut(s) 169, 221, 1015, 1060
BssECI CCNNGG 5 cut(s) 22, 367, 401, 867, 1364
BssMI GATC 7 cut(s) 840, 1003, 1048, 1088, 1425, 1567, 1922
BssT1I CCWWGG 3 cut(s) 401, 867, 1364
Bst2UI CCWGG 2 cut(s) 24, 369
Bst4CI ACNGT 6 cut(s) 763, 992, 1123, 1130, 1237, 1912
Bst6I CTCTTC 4 cut(s) 429, 1579, 1676, 1813
BstAPI GCANNNNNTGC 1 cut(s) 947
BstBAI YACGTR 1 cut(s) 944
BstC8I GCNNGC 2 cut(s) 772, 1997
BstDEI CTNAG 7 cut(s) 378, 540, 617, 1175, 1249, 1696, 1725
BstDSI CCRYGG 1 cut(s) 867
BstFNI CGCG 2 cut(s) 676, 1789
BstKTI GATC 7 cut(s) 843, 1006, 1051, 1091, 1428, 1570, 1925
BstMAI GTCTC 5 cut(s) 200, 1243, 1467, 1744, 1983
BstMBI GATC 7 cut(s) 840, 1003, 1048, 1088, 1425, 1567, 1922
BstMCI CGRYCG 1 cut(s) 1428
BstMWI GCNNNNNNNGC 3 cut(s) 947, 1284, 1992
BstNI CCWGG 2 cut(s) 24, 369
BstNSI RCATGY 3 cut(s) 774, 778, 1480
BstSCI CCNGG 4 cut(s) 22, 367, 929, 1981
BstSFI CTRYAG 1 cut(s) 1894
BstSLI GKGCMC 4 cut(s) 943, 1017, 1062, 1135
BstUI CGCG 2 cut(s) 676, 1789
BstV1I GCAGC 1 cut(s) 193
BstV2I GAAGAC 1 cut(s) 693
BstXI CCANNNNNNTGG 3 cut(s) 958, 1326, 1588
BsuRI GGCC 5 cut(s) 756, 1231, 1317, 1377, 1999
BtgI CCRYGG 1 cut(s) 867
BtsI GCAGTG 1 cut(s) 321
BtsIMutI CAGTG 6 cut(s) 54, 321, 363, 997, 1158, 1233
Cac8I GCNNGC 2 cut(s) 772, 1997
CciI TCATGA 1 cut(s) 1537
Cfr13I GGNCC 9 cut(s) 238, 405, 961, 977, 1230, 1316, 1376, 1823, 2016
Csp6I GTAC 1 cut(s) 494
CspCI CAANNNNNGTGG 4 cut(s) 339, 374, 1816, 1851
CviQI GTAC 1 cut(s) 494
DdeI CTNAG 7 cut(s) 378, 540, 617, 1175, 1249, 1696, 1725
DpnI GATC 7 cut(s) 842, 1005, 1050, 1090, 1427, 1569, 1924
DpnII GATC 7 cut(s) 840, 1003, 1048, 1088, 1425, 1567, 1922
DraI TTTAAA 1 cut(s) 1312
DraIII CACNNNGTG 1 cut(s) 947
EaeI YGGCCR 1 cut(s) 754
Eam1104I CTCTTC 4 cut(s) 429, 1579, 1676, 1813
EarI CTCTTC 4 cut(s) 429, 1579, 1676, 1813
EciI GGCGGA 1 cut(s) 931
Ecl136II GAGCTC 2 cut(s) 294, 544
Eco130I CCWWGG 3 cut(s) 401, 867, 1364
Eco24I GRGCYC 3 cut(s) 296, 546, 1222
Eco47I GGWCC 6 cut(s) 238, 405, 961, 977, 1823, 2016
Eco53kI GAGCTC 2 cut(s) 294, 544
Eco57I CTGAAG 4 cut(s) 354, 446, 1560, 1700
Eco88I CYCGRG 1 cut(s) 261
EcoICRI GAGCTC 2 cut(s) 294, 544
EcoO109I RGGNCCY 3 cut(s) 238, 1823, 2016
EcoRII CCWGG 2 cut(s) 22, 367
EcoT14I CCWWGG 3 cut(s) 401, 867, 1364
EcoT22I ATGCAT 2 cut(s) 772, 776
EcoT38I GRGCYC 3 cut(s) 296, 546, 1222
ErhI CCWWGG 3 cut(s) 401, 867, 1364
FalI AAGNNNNNCTT 4 cut(s) 327, 359, 1128, 1160
FaqI GGGAC 2 cut(s) 1809, 2002
FblI GTMKAC 1 cut(s) 1893
Fnu4HI GCNGC 5 cut(s) 182, 674, 914, 1993, 2010
FriOI GRGCYC 3 cut(s) 296, 546, 1222
Fsp4HI GCNGC 5 cut(s) 182, 674, 914, 1993, 2010
FspBI CTAG 8 cut(s) 185, 1200, 1215, 1275, 1400, 1565, 1794, 1850
GluI GCNGC 5 cut(s) 182, 674, 914, 1993, 2010
GsaI CCCAGC 1 cut(s) 1607
GsuI CTGGAG 1 cut(s) 1779
HaeIII GGCC 5 cut(s) 756, 1231, 1317, 1377, 1999
HapII CCGG 3 cut(s) 851, 931, 1982
HincII GTYRAC 1 cut(s) 34
HindII GTYRAC 1 cut(s) 34
HindIII AAGCTT 1 cut(s) 96
HinfI GANTC 7 cut(s) 71, 193, 663, 821, 892, 919, 1157
HpaI GTTAAC 1 cut(s) 34
HpaII CCGG 3 cut(s) 851, 931, 1982
HphI GGTGA 6 cut(s) 37, 517, 1478, 1522, 1651, 1656
Hpy166II GTNNAC 4 cut(s) 34, 361, 1126, 1894
Hpy8I GTNNAC 4 cut(s) 34, 361, 1126, 1894
HpyAV CCTTC 5 cut(s) 649, 1758, 1797, 1854, 1913
HpyCH4III ACNGT 6 cut(s) 763, 992, 1123, 1130, 1237, 1912
HpyCH4IV ACGT 1 cut(s) 943
HpyCH4V TGCA 6 cut(s) 181, 746, 770, 774, 1022, 1845
HpyF10VI GCNNNNNNNGC 3 cut(s) 947, 1284, 1992
HpyF3I CTNAG 7 cut(s) 378, 540, 617, 1175, 1249, 1696, 1725
HpySE526I ACGT 1 cut(s) 943
KspAI GTTAAC 1 cut(s) 34
Kzo9I GATC 7 cut(s) 840, 1003, 1048, 1088, 1425, 1567, 1922
LmnI GCTCC 5 cut(s) 311, 853, 1561, 1734, 1786
Lsp1109I GCAGC 1 cut(s) 193
LweI GCATC 3 cut(s) 660, 900, 1123
MaeI CTAG 8 cut(s) 185, 1200, 1215, 1275, 1400, 1565, 1794, 1850
MaeII ACGT 1 cut(s) 943
MaeIII GTNAC 1 cut(s) 1906
MalI GATC 7 cut(s) 842, 1005, 1050, 1090, 1427, 1569, 1924
MboI GATC 7 cut(s) 840, 1003, 1048, 1088, 1425, 1567, 1922
MboII GAAGA 9 cut(s) 347, 404, 439, 446, 698, 1566, 1693, 1827, 1830
MfeI CAATTG 2 cut(s) 267, 456
MhlI GDGCHC 8 cut(s) 296, 546, 943, 1017, 1062, 1135, 1222, 1783
MlsI TGGCCA 1 cut(s) 756
MluNI TGGCCA 1 cut(s) 756
MlyI GAGTC 4 cut(s) 65, 202, 886, 928
MmeI TCCRAC 2 cut(s) 914, 1776
Mox20I TGGCCA 1 cut(s) 756
Mph1103I ATGCAT 2 cut(s) 772, 776
MroXI GAANNNNTTC 1 cut(s) 305
MscI TGGCCA 1 cut(s) 756
MseI TTAA 5 cut(s) 33, 1257, 1311, 1610, 1914
MslI CAYNNNNRTG 4 cut(s) 598, 956, 1455, 1586
Msp20I TGGCCA 1 cut(s) 756
MspI CCGG 3 cut(s) 851, 931, 1982
MspR9I CCNGG 4 cut(s) 24, 369, 931, 1983
MunI CAATTG 2 cut(s) 267, 456
Mva1269I GAATGC 2 cut(s) 384, 772
MvaI CCWGG 2 cut(s) 24, 369
MvnI CGCG 2 cut(s) 676, 1789
MwoI GCNNNNNNNGC 3 cut(s) 947, 1284, 1992
NciI CCSGG 2 cut(s) 931, 1983
NcoI CCATGG 1 cut(s) 867
NdeII GATC 7 cut(s) 840, 1003, 1048, 1088, 1425, 1567, 1922
NlaIV GGNNCC 8 cut(s) 239, 802, 855, 978, 1016, 1061, 1825, 2018
NsiI ATGCAT 2 cut(s) 772, 776
NspI RCATGY 3 cut(s) 774, 778, 1480
OliI CACNNNNGTG 1 cut(s) 598
PaeI GCATGC 1 cut(s) 774
PaeR7I CTCGAG 1 cut(s) 261
PagI TCATGA 1 cut(s) 1537
PctI GAATGC 2 cut(s) 384, 772
PdmI GAANNNNTTC 1 cut(s) 305
PfeI GAWTC 3 cut(s) 663, 821, 1157
PkrI GCNGC 5 cut(s) 183, 675, 915, 1994, 2011
Ple19I CGATCG 1 cut(s) 1428
PleI GAGTC 4 cut(s) 65, 201, 886, 927
PpsI GAGTC 4 cut(s) 65, 201, 886, 927
Ppu21I YACGTR 1 cut(s) 944
PpuMI RGGWCCY 3 cut(s) 238, 1823, 2016
Psp124BI GAGCTC 2 cut(s) 296, 546
Psp5II RGGWCCY 3 cut(s) 238, 1823, 2016
Psp6I CCWGG 2 cut(s) 22, 367
PspFI CCCAGC 1 cut(s) 1603
PspGI CCWGG 2 cut(s) 22, 367
PspN4I GGNNCC 8 cut(s) 239, 802, 855, 978, 1016, 1061, 1825, 2018
PspPI GGNCC 9 cut(s) 238, 405, 961, 977, 1230, 1316, 1376, 1823, 2016
PspPPI RGGWCCY 3 cut(s) 238, 1823, 2016
PspXI VCTCGAGB 1 cut(s) 261
PvuI CGATCG 1 cut(s) 1428
RsaI GTAC 1 cut(s) 495
RsaNI GTAC 1 cut(s) 494
RseI CAYNNNNRTG 4 cut(s) 598, 956, 1455, 1586
SacI GAGCTC 2 cut(s) 296, 546
SaqAI TTAA 5 cut(s) 33, 1257, 1311, 1610, 1914
SatI GCNGC 5 cut(s) 182, 674, 914, 1993, 2010
Sau3AI GATC 7 cut(s) 840, 1003, 1048, 1088, 1425, 1567, 1922
Sau96I GGNCC 9 cut(s) 238, 405, 961, 977, 1230, 1316, 1376, 1823, 2016
SchI GAGTC 4 cut(s) 65, 202, 886, 928
ScrFI CCNGG 4 cut(s) 24, 369, 931, 1983
SduI GDGCHC 8 cut(s) 296, 546, 943, 1017, 1062, 1135, 1222, 1783
SfaNI GCATC 3 cut(s) 660, 900, 1123
SfcI CTRYAG 1 cut(s) 1894
Sfr274I CTCGAG 1 cut(s) 261
SinI GGWCC 6 cut(s) 238, 405, 961, 977, 1823, 2016
SlaI CTCGAG 1 cut(s) 261
SmiMI CAYNNNNRTG 4 cut(s) 598, 956, 1455, 1586
SmlI CTYRAG 2 cut(s) 261, 1942
SmoI CTYRAG 2 cut(s) 261, 1942
SpeI ACTAGT 1 cut(s) 1199
SphI GCATGC 1 cut(s) 774
SsiI CCGC 8 cut(s) 304, 674, 913, 916, 1370, 1986, 1992, 2010
SspMI CTAG 8 cut(s) 185, 1200, 1215, 1275, 1400, 1565, 1794, 1850
SstI GAGCTC 2 cut(s) 296, 546
StyD4I CCNGG 4 cut(s) 22, 367, 929, 1981
StyI CCWWGG 3 cut(s) 401, 867, 1364
TaaI ACNGT 6 cut(s) 763, 992, 1123, 1130, 1237, 1912
TaiI ACGT 1 cut(s) 946
TaqI TCGA 2 cut(s) 262, 636
TauI GCSGC 4 cut(s) 676, 916, 1995, 2012
TfiI GAWTC 3 cut(s) 663, 821, 1157
Tru1I TTAA 5 cut(s) 33, 1257, 1311, 1610, 1914
Tru9I TTAA 5 cut(s) 33, 1257, 1311, 1610, 1914
TscAI CASTG 6 cut(s) 61, 321, 363, 997, 1158, 1240
TseI GCWGC 1 cut(s) 181
TspDTI ATGAA 5 cut(s) 570, 699, 807, 1503, 1932
TspRI CASTG 6 cut(s) 61, 321, 363, 997, 1158, 1240
VpaK11BI GGWCC 6 cut(s) 238, 405, 961, 977, 1823, 2016
XapI RAATTY 4 cut(s) 567, 794, 815, 1297
XbaI TCTAGA 3 cut(s) 1214, 1793, 1849
XceI RCATGY 3 cut(s) 774, 778, 1480
XcmI CCANNNNNNNNNTGG 1 cut(s) 1460
XhoI CTCGAG 1 cut(s) 261
XmiI GTMKAC 1 cut(s) 1893
XmnI GAANNNNTTC 1 cut(s) 305
XspI CTAG 8 cut(s) 185, 1200, 1215, 1275, 1400, 1565, 1794, 1850
Zsp2I ATGCAT 2 cut(s) 772, 776
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.