Domain of unknown function (DUF4283)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 1 cut(s) | 741 |
| AciI | CCGC | 4 cut(s) | 140, 148, 293, 741 |
| AclWI | GGATC | 6 cut(s) | 139, 358, 371, 396, 535, 548 |
| AcoI | YGGCCR | 1 cut(s) | 388 |
| AcsI | RAATTY | 3 cut(s) | 49, 122, 575 |
| AfaI | GTAC | 1 cut(s) | 339 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 265, 537, 731 |
| AgsI | TTSAA | 3 cut(s) | 20, 357, 452 |
| AjnI | CCWGG | 1 cut(s) | 272 |
| AluBI | AGCT | 4 cut(s) | 71, 323, 371, 425 |
| AluI | AGCT | 4 cut(s) | 71, 323, 371, 425 |
| AlwI | GGATC | 6 cut(s) | 139, 358, 371, 396, 535, 548 |
| AoxI | GGCC | 3 cut(s) | 269, 388, 625 |
| ApaI | GGGCCC | 1 cut(s) | 273 |
| ApeKI | GCWGC | 1 cut(s) | 137 |
| ApoI | RAATTY | 3 cut(s) | 49, 122, 575 |
| AseI | ATTAAT | 1 cut(s) | 468 |
| AspA2I | CCTAGG | 1 cut(s) | 100 |
| AspS9I | GGNCC | 5 cut(s) | 269, 270, 343, 625, 748 |
| AsuC2I | CCSGG | 1 cut(s) | 531 |
| AsuHPI | GGTGA | 4 cut(s) | 154, 215, 292, 517 |
| AvaII | GGWCC | 2 cut(s) | 343, 748 |
| AvrII | CCTAGG | 1 cut(s) | 100 |
| AxyI | CCTNAGG | 1 cut(s) | 488 |
| BaeGI | GKGCMC | 1 cut(s) | 273 |
| BalI | TGGCCA | 1 cut(s) | 390 |
| BamHI | GGATCC | 2 cut(s) | 363, 540 |
| BanII | GRGCYC | 1 cut(s) | 273 |
| BbsI | GAAGAC | 1 cut(s) | 459 |
| BbvI | GCAGC | 1 cut(s) | 124 |
| BccI | CCATC | 4 cut(s) | 236, 312, 354, 725 |
| BciT130I | CCWGG | 1 cut(s) | 274 |
| BcnI | CCSGG | 1 cut(s) | 531 |
| BfaI | CTAG | 2 cut(s) | 101, 155 |
| BglII | AGATCT | 1 cut(s) | 55 |
| BisI | GCNGC | 2 cut(s) | 138, 742 |
| BlnI | CCTAGG | 1 cut(s) | 100 |
| BlsI | GCNGC | 2 cut(s) | 139, 743 |
| Bme1390I | CCNGG | 2 cut(s) | 274, 531 |
| Bme18I | GGWCC | 2 cut(s) | 343, 748 |
| BmgT120I | GGNCC | 5 cut(s) | 269, 270, 343, 625, 748 |
| BmiI | GGNNCC | 5 cut(s) | 270, 271, 365, 542, 749 |
| BmrFI | CCNGG | 2 cut(s) | 274, 531 |
| BmrI | ACTGGG | 1 cut(s) | 3 |
| BmsI | GCATC | 3 cut(s) | 159, 513, 600 |
| BmuI | ACTGGG | 1 cut(s) | 3 |
| BpiI | GAAGAC | 1 cut(s) | 459 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 471, 503 |
| BpmI | CTGGAG | 1 cut(s) | 496 |
| BpuEI | CTTGAG | 1 cut(s) | 464 |
| BpuMI | CCSGG | 1 cut(s) | 531 |
| BsaBI | GATNNNNATC | 1 cut(s) | 149 |
| BsaJI | CCNNGG | 4 cut(s) | 100, 272, 529, 662 |
| BsaWI | WCCGGW | 1 cut(s) | 543 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 265, 537, 731 |
| Bse1I | ACTGG | 3 cut(s) | 9, 321, 333 |
| Bse21I | CCTNAGG | 1 cut(s) | 488 |
| Bse8I | GATNNNNATC | 1 cut(s) | 149 |
| BseBI | CCWGG | 1 cut(s) | 274 |
| BseDI | CCNNGG | 4 cut(s) | 100, 272, 529, 662 |
| BseGI | GGATG | 4 cut(s) | 150, 337, 658, 693 |
| BseJI | GATNNNNATC | 1 cut(s) | 149 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 265, 537, 731 |
| BseMII | CTCAG | 1 cut(s) | 502 |
| BseNI | ACTGG | 3 cut(s) | 9, 321, 333 |
| BseSI | GKGCMC | 1 cut(s) | 273 |
| BseXI | GCAGC | 1 cut(s) | 124 |
| BseYI | CCCAGC | 2 cut(s) | 676, 744 |
| Bsh1236I | CGCG | 1 cut(s) | 741 |
| BshFI | GGCC | 3 cut(s) | 271, 390, 627 |
| BsiSI | CCGG | 2 cut(s) | 531, 544 |
| BslFI | GGGAC | 1 cut(s) | 761 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 265, 537, 731 |
| BsmFI | GGGAC | 1 cut(s) | 761 |
| BsnI | GGCC | 3 cut(s) | 271, 390, 627 |
| Bsp120I | GGGCCC | 1 cut(s) | 269 |
| Bsp1286I | GDGCHC | 1 cut(s) | 273 |
| Bsp1407I | TGTACA | 1 cut(s) | 337 |
| Bsp143I | GATC | 5 cut(s) | 55, 144, 363, 401, 540 |
| BspACI | CCGC | 4 cut(s) | 140, 148, 293, 741 |
| BspANI | GGCC | 3 cut(s) | 271, 390, 627 |
| BspCNI | CTCAG | 1 cut(s) | 501 |
| BspFNI | CGCG | 1 cut(s) | 741 |
| BspLI | GGNNCC | 5 cut(s) | 270, 271, 365, 542, 749 |
| BspPI | GGATC | 6 cut(s) | 139, 358, 371, 396, 535, 548 |
| BsrGI | TGTACA | 1 cut(s) | 337 |
| BsrI | ACTGG | 3 cut(s) | 9, 321, 333 |
| BssECI | CCNNGG | 4 cut(s) | 100, 272, 529, 662 |
| BssMI | GATC | 5 cut(s) | 55, 144, 363, 401, 540 |
| BssT1I | CCWWGG | 2 cut(s) | 100, 662 |
| Bst2UI | CCWGG | 1 cut(s) | 274 |
| Bst4CI | ACNGT | 3 cut(s) | 614, 719, 752 |
| BstAUI | TGTACA | 1 cut(s) | 337 |
| BstC8I | GCNNGC | 1 cut(s) | 635 |
| BstDEI | CTNAG | 2 cut(s) | 488, 494 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 263 |
| BstF5I | GGATG | 4 cut(s) | 150, 337, 658, 693 |
| BstFNI | CGCG | 1 cut(s) | 741 |
| BstKTI | GATC | 5 cut(s) | 58, 147, 366, 404, 543 |
| BstMBI | GATC | 5 cut(s) | 55, 144, 363, 401, 540 |
| BstNI | CCWGG | 1 cut(s) | 274 |
| BstSCI | CCNGG | 2 cut(s) | 272, 529 |
| BstSLI | GKGCMC | 1 cut(s) | 273 |
| BstUI | CGCG | 1 cut(s) | 741 |
| BstV1I | GCAGC | 1 cut(s) | 124 |
| BstV2I | GAAGAC | 1 cut(s) | 459 |
| BstX2I | RGATCY | 4 cut(s) | 55, 363, 401, 540 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 374 |
| BstYI | RGATCY | 4 cut(s) | 55, 363, 401, 540 |
| Bsu36I | CCTNAGG | 1 cut(s) | 488 |
| BsuRI | GGCC | 3 cut(s) | 271, 390, 627 |
| BtsCI | GGATG | 4 cut(s) | 150, 337, 658, 693 |
| Cac8I | GCNNGC | 1 cut(s) | 635 |
| Cfr13I | GGNCC | 5 cut(s) | 269, 270, 343, 625, 748 |
| Csp6I | GTAC | 1 cut(s) | 338 |
| CviAII | CATG | 2 cut(s) | 41, 374 |
| CviQI | GTAC | 1 cut(s) | 338 |
| DdeI | CTNAG | 2 cut(s) | 488, 494 |
| DpnI | GATC | 5 cut(s) | 57, 146, 365, 403, 542 |
| DpnII | GATC | 5 cut(s) | 55, 144, 363, 401, 540 |
| EaeI | YGGCCR | 1 cut(s) | 388 |
| Eco130I | CCWWGG | 2 cut(s) | 100, 662 |
| Eco24I | GRGCYC | 1 cut(s) | 273 |
| Eco47I | GGWCC | 2 cut(s) | 343, 748 |
| Eco81I | CCTNAGG | 1 cut(s) | 488 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 263 |
| EcoO109I | RGGNCCY | 2 cut(s) | 269, 270 |
| EcoRI | GAATTC | 1 cut(s) | 575 |
| EcoRII | CCWGG | 1 cut(s) | 272 |
| EcoT14I | CCWWGG | 2 cut(s) | 100, 662 |
| EcoT38I | GRGCYC | 1 cut(s) | 273 |
| ErhI | CCWWGG | 2 cut(s) | 100, 662 |
| FaeI | CATG | 2 cut(s) | 44, 377 |
| FaqI | GGGAC | 1 cut(s) | 761 |
| FatI | CATG | 2 cut(s) | 40, 373 |
| Fnu4HI | GCNGC | 2 cut(s) | 138, 742 |
| FokI | GGATG | 4 cut(s) | 137, 344, 645, 700 |
| FriOI | GRGCYC | 1 cut(s) | 273 |
| Fsp4HI | GCNGC | 2 cut(s) | 138, 742 |
| FspBI | CTAG | 2 cut(s) | 101, 155 |
| GluI | GCNGC | 2 cut(s) | 138, 742 |
| GsaI | CCCAGC | 2 cut(s) | 680, 748 |
| GsuI | CTGGAG | 1 cut(s) | 496 |
| HaeIII | GGCC | 3 cut(s) | 271, 390, 627 |
| HapII | CCGG | 2 cut(s) | 531, 544 |
| Hin1II | CATG | 2 cut(s) | 44, 377 |
| HinfI | GANTC | 2 cut(s) | 194, 553 |
| HpaII | CCGG | 2 cut(s) | 531, 544 |
| HphI | GGTGA | 4 cut(s) | 154, 215, 292, 517 |
| Hpy188I | TCNGA | 6 cut(s) | 34, 213, 231, 699, 730, 759 |
| Hpy188III | TCNNGA | 4 cut(s) | 74, 361, 443, 538 |
| HpyCH4III | ACNGT | 3 cut(s) | 614, 719, 752 |
| HpyCH4V | TGCA | 1 cut(s) | 464 |
| HpyF3I | CTNAG | 2 cut(s) | 488, 494 |
| Hsp92II | CATG | 2 cut(s) | 44, 377 |
| Kzo9I | GATC | 5 cut(s) | 55, 144, 363, 401, 540 |
| LmnI | GCTCC | 2 cut(s) | 422, 650 |
| Lsp1109I | GCAGC | 1 cut(s) | 124 |
| LweI | GCATC | 3 cut(s) | 159, 513, 600 |
| MaeI | CTAG | 2 cut(s) | 101, 155 |
| MaeIII | GTNAC | 2 cut(s) | 11, 515 |
| MalI | GATC | 5 cut(s) | 57, 146, 365, 403, 542 |
| MboI | GATC | 5 cut(s) | 55, 144, 363, 401, 540 |
| MboII | GAAGA | 2 cut(s) | 464, 630 |
| MflI | RGATCY | 4 cut(s) | 55, 363, 401, 540 |
| MhlI | GDGCHC | 1 cut(s) | 273 |
| MlsI | TGGCCA | 1 cut(s) | 390 |
| MluCI | AATT | 5 cut(s) | 49, 122, 465, 498, 575 |
| MluNI | TGGCCA | 1 cut(s) | 390 |
| MmeI | TCCRAC | 1 cut(s) | 722 |
| Mox20I | TGGCCA | 1 cut(s) | 390 |
| MscI | TGGCCA | 1 cut(s) | 390 |
| MseI | TTAA | 1 cut(s) | 468 |
| Msp20I | TGGCCA | 1 cut(s) | 390 |
| MspI | CCGG | 2 cut(s) | 531, 544 |
| MspR9I | CCNGG | 2 cut(s) | 274, 531 |
| MvaI | CCWGG | 1 cut(s) | 274 |
| MvnI | CGCG | 1 cut(s) | 741 |
| NciI | CCSGG | 1 cut(s) | 531 |
| NdeII | GATC | 5 cut(s) | 55, 144, 363, 401, 540 |
| NlaIII | CATG | 2 cut(s) | 44, 377 |
| NlaIV | GGNNCC | 5 cut(s) | 270, 271, 365, 542, 749 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 715 |
| PfeI | GAWTC | 2 cut(s) | 194, 553 |
| PkrI | GCNGC | 2 cut(s) | 139, 743 |
| PshBI | ATTAAT | 1 cut(s) | 468 |
| Psp6I | CCWGG | 1 cut(s) | 272 |
| PspFI | CCCAGC | 2 cut(s) | 676, 744 |
| PspGI | CCWGG | 1 cut(s) | 272 |
| PspN4I | GGNNCC | 5 cut(s) | 270, 271, 365, 542, 749 |
| PspOMI | GGGCCC | 1 cut(s) | 269 |
| PspPI | GGNCC | 5 cut(s) | 269, 270, 343, 625, 748 |
| PsuI | RGATCY | 4 cut(s) | 55, 363, 401, 540 |
| RsaI | GTAC | 1 cut(s) | 339 |
| RsaNI | GTAC | 1 cut(s) | 338 |
| SaqAI | TTAA | 1 cut(s) | 468 |
| SatI | GCNGC | 2 cut(s) | 138, 742 |
| Sau3AI | GATC | 5 cut(s) | 55, 144, 363, 401, 540 |
| Sau96I | GGNCC | 5 cut(s) | 269, 270, 343, 625, 748 |
| ScrFI | CCNGG | 2 cut(s) | 274, 531 |
| SduI | GDGCHC | 1 cut(s) | 273 |
| SfaNI | GCATC | 3 cut(s) | 159, 513, 600 |
| SinI | GGWCC | 2 cut(s) | 343, 748 |
| SmlI | CTYRAG | 1 cut(s) | 443 |
| SmoI | CTYRAG | 1 cut(s) | 443 |
| Sse9I | AATT | 5 cut(s) | 49, 122, 465, 498, 575 |
| SsiI | CCGC | 4 cut(s) | 140, 148, 293, 741 |
| SspMI | CTAG | 2 cut(s) | 101, 155 |
| StyD4I | CCNGG | 2 cut(s) | 272, 529 |
| StyI | CCWWGG | 2 cut(s) | 100, 662 |
| TaaI | ACNGT | 3 cut(s) | 614, 719, 752 |
| TaqI | TCGA | 1 cut(s) | 192 |
| TasI | AATT | 5 cut(s) | 49, 122, 465, 498, 575 |
| TatI | WGTACW | 1 cut(s) | 337 |
| TauI | GCSGC | 1 cut(s) | 744 |
| TfiI | GAWTC | 2 cut(s) | 194, 553 |
| Tru1I | TTAA | 1 cut(s) | 468 |
| Tru9I | TTAA | 1 cut(s) | 468 |
| TseI | GCWGC | 1 cut(s) | 137 |
| TspDTI | ATGAA | 2 cut(s) | 17, 600 |
| VpaK11BI | GGWCC | 2 cut(s) | 343, 748 |
| VspI | ATTAAT | 1 cut(s) | 468 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 263 |
| XapI | RAATTY | 3 cut(s) | 49, 122, 575 |
| XmaJI | CCTAGG | 1 cut(s) | 100 |
| XspI | CTAG | 2 cut(s) | 101, 155 |