RLG00000031112

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000073
Physical Location & Seq
Forward (+)
2216378 .. 2222321
5944 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031112

Sequence Viewer

Length: 2517 bp
ATGTCTTCGCATAACCACCTTGCCTCCAGGTTGGAAAACACCTCCCTTGATGAGATTGAAAACGAGGTCTCTCTGCTTGGTGCAATAATTGCAGATGTGGTACCAAACATAGGAGGAATAAAGGGTTCACTCCGAACCTCCTGGAGGAATCTTGGTGCAATCAAAGTGGCACACATCCACCACAACATCTTCTCCATAAAGGCATCACCTGCAGATGCAACAACCCTCCTTGATGGTGGCCCCTGGCATGTTGGCAACATGAGATTCAATGTGGTCAAATGGTTGCCAACACTCACTATCAGTGATGTTCATTTCCACATGGTCTCGTATTGGGTTCAGATATCCGGGCTTACAAGGGAGAAAATGAATACAAAGAATGCTTTGTTGATCGGCTCGGAGATTGGAACTGTTTTGGAAGTGGAAGACCCAAGCGTTGTTGATGCTACTCTTAGGGGCTACCTGAGGGTAAAAGTCCTCTTGGATTCCAGAAACCCACTACCAACTGGTTTCTGGCTTCCTCTCAATGAATACACTTCTACAAGAGTCGATTATGCCTTTGAGGGGCTATGTGATTTTTGCTACAAATGTGGGAAACTTGGCCACCATTTAGATGTCTGCAAAAATTTCCGAATCCTCAATCTGGAGGATGGTGAACCAGAGAATATCTGGTATGGTCCATGGATGAAGACGAAGTCCGTCTCGGTGAAGCATCTTTCTTGGCTTGACCGGCCAGAGCGAGTTAATCGGAGAAAGCCCAAGACCAAGAGGGTCACGGGTACTTACCCAGATTCCCCATACCCATCAGTGGCTGGTCCAAGTCGTCAAACCCAAGGTACCAGGCATAATTCAAACTCTGTAGACATCGACCTGTCTCTCCCTCTGATCTCTGAACCAGCTGATGGTGACATGACCTCCCAAATTTCAAATTCAATTTGCCCCCCCATGTTACACAATTATCCAAAGGACAAGCTGCTCCCGACCCCACCGGTGACCCATGCCTCATCTCTGAAGGTACACCCATTTCCTAGCCCTAACTGGCTAAAACTTGCTGTCACAACAACCAAAACTCATGTCGAGTGGACTAGAAGAGTCAATCCCCCACCAATGAGTGTCAAACCATGGCCCAATGGGTCACAACCCTCAGTGGCTGATAATCCTGAGGCCCAAGCCACTTTAGGCCCAAGCCACTTCAGGCCTGTATTACTGGGGAATGGTAAGGGAAAACCCCCAACGTACCGTTTTTCTCCTACCTCTCTTGATCAGATTCAGACTTTAGCAATTAAACCTCCTCACCCGACTCAACCAACCATCCACCCAACTTTTAAGTCCTCACCCCCATTTCACCCATCAGCCAAAAGACTGTACTCTCAAACCTTTACCACCACAAACCCAAGACATCCCAAAACCCGTTCTCTTTCAACACTCAACTCCAATTCTACTTCGGAGGACAATTGGGACCCAACTCCCCCGGTTCTTACCCCTTCCAAAGCTGGTCTTTCAAGAGGCAGAGGTCGCCCACGTGGTAGAGGCAGAGGAGCCGGTGGGAGGCACTCAGTGGTTTCACCTCCGGTAACCAGAAGTGGCAGATTTGTTTCGAAAAAAAGGAGGTTTGATCCTTCCCCAAGCTCTGAACACAATTTGGGTGAATTTCAAGAGATTAGAGTCGAATGTTTAGATGACTCGTCAGAATTACGTTACCCCGGACAGGATAGATGGGTTCCTAATGGTAATGGTGGTCTGATCAAGCCAATTTTCACCTCCAATAGATTTACTCCCCTCCTTGTTTCGGAAATCATTGACGATAATAGAAGCTGGAACATTCAGCACCTGGAACCATTTTTGGAGACCTCTGATATAAGAGCTATAAAGGCTATCCCAATTGGGAATAGCAATGATGAAGATTCTTTGATCTGGCCCCATACAAAAAATGGCTCTTACTCAGTAAAAAGTGGATACAACAAGTTGTTTTCTTCTGTTACGTTGGAGACAAATTGCAAGCCCTCTTCTTCTTCTCAGCCCCCTCCCCCACCCTTTGTCAAGATTAATGTGGATGGAGCGTGGGATGAGGTGAATGGTAATAGTGGAATGGGAATTATTATCAGGAATCATAGCGGCCACTCAATTGCTGGAGCGAGTTTACATGGAAATCACAATTCTGCAGTAGAAGTGGAAGCTGAGGCTATTGTAAAGGGCCTCCAGCTGGCTGAGTTCATGAAGTGTAGAAATATCATTCTTGAAAGTGATTGTAAAGAAGTTATTTGTGCTATGGACAACCCGGATTTCACCCCAAATTGGAGAATTGTCCCAATGCTTAACAGGGTGACACATTTGAAGACTCTTTTTGACAGGATCAAGTTGTTTTGGATCCCTCGCGAAACTAACCGTGTTGCGGATGCAGCGGCAAAGTTAGCAAAGCTGAGGTTGTGCTCCCAGGACTGGGCCAATAGGCCTCCAACCTCCCTTCTGGCTGTGTTAAGGAATGATGGTCTCCCAGGGCCCCCTAGTAGCTCTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

839

Amino Acids

92.87

Weight (kDa)

9.33

Isoelectric Point (pI)

43.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4283 PF14111 21 - 99 2.5e-07 Domain of unknown function (DUF4283)
RVT_3 PF13456 682 - 804 2e-24 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000073)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g17821 FvH4_2g04610 FvH4_2g09611 FvH4_2g13563 FvH4_3g21493 FvH4_3g32115 FvH4_3g35060 FvH4_4g00220 FvH4_4g06410 FvH4_4g10367 FvH4_4g10367 FvH4_4g10367 FvH4_5g06101 FvH4_5g08842 FvH4_5g27366 FvH4_5g31283 FvH4_6g11611 FvH4_6g23032 FvH4_7g05661 FvH4_7g06172
malus_domestica MD13G1263700.v1.1
prunus_persica Prupe.1G292300_v2.0.a1 Prupe.5G009700_v2.0.a1 Prupe.6G158100_v2.0.a1 Prupe.8G025100_v2.0.a1 Prupe.8G042600_v2.0.a1
pyrus_communis pycom02g02250 pycom02g16030 pycom05g10490 pycom09g06450 pycom09g06460 pycom13g26200 pycom16g17700
rosa_chinensis RchiOBHm_Chr2g0163321 RchiOBHm_Chr4g0401981 RchiOBHm_Chr5g0005991 RchiOBHm_Chr5g0027191 RchiOBHm_Chr6g0249771
rosa_laevigata RLG00000001040 RLG00000001764 RLG00000002239 RLG00000002240 RLG00000003259 RLG00000008993 RLG00000013847 RLG00000014178 RLG00000015576 RLG00000017269 RLG00000017355 RLG00000018127 RLG00000018602 RLG00000018615 RLG00000019904 RLG00000020044 RLG00000021849 RLG00000021850 RLG00000027965 RLG00000029266 RLG00000029820 RLG00000031112 RLG00000031915 RLG00000033568 RLG00000035013 RLG00000035677 RLG00000036568 RLG00000036628
rosa_multiflora Rmu_co8254085.1_g000001 Rmu_co8462383.1_g000001 Rmu_sc0000185.1_g000049 Rmu_sc0000251.1_g000018 Rmu_sc0000367.1_g000094 Rmu_sc0000498.1_g000023 Rmu_sc0000868.1_g000005 Rmu_sc0001521.1_g000047 Rmu_sc0001534.1_g000007 Rmu_sc0002014.1_g000012 Rmu_sc0003214.1_g000009 Rmu_sc0003573.1_g000041 Rmu_sc0003832.1_g000023 Rmu_sc0004677.1_g000015 Rmu_sc0004967.1_g000014 Rmu_sc0005426.1_g000001 Rmu_sc0005621.1_g000004 Rmu_sc0005640.1_g000009 Rmu_sc0005947.1_g000034 Rmu_sc0006889.1_g000011 Rmu_sc0007121.1_g000014 Rmu_sc0009191.1_g000017 Rmu_sc0010556.1_g000011 Rmu_sc0011396.1_g000007 Rmu_sc0019862.1_g000002 Rmu_sc0022931.1_g000003 Rmu_sc0024164.1_g000001 Rmu_ssc0000391.1_g000027
rosa_roxburghii Rroxscaffold_1G00050060 Rroxscaffold_1G00061810 Rroxscaffold_2G00109590 Rroxscaffold_2G00113810 Rroxscaffold_2G00124040 Rroxscaffold_2G00143620 Rroxscaffold_2G00154760 Rroxscaffold_4G00302820 Rroxscaffold_4G00302830 Rroxscaffold_4G00308680 Rroxscaffold_4G00310240 Rroxscaffold_4G00327890 Rroxscaffold_5G00334600 Rroxscaffold_5G00336050 Rroxscaffold_5G00346560 Rroxscaffold_5G00347860 Rroxscaffold_5G00347870 Rroxscaffold_5G00349330 Rroxscaffold_5G00351980 Rroxscaffold_6G00395280 Rroxscaffold_6G00396750 Rroxscaffold_6G00402910 Rroxscaffold_7G00174790 Rroxscaffold_7G00177570 Rroxscaffold_7G00191820 Rroxscaffold_7G00203480 Rroxscaffold_7G00204380
rosa_rugosa Rorug01G0106600 Rorug01G0106600 Rorug01G0148500.1 Rorug02G0224200 Rorug02G0225500 Rorug02G0233000 Rorug02G0630400 Rorug03G0198000 Rorug03G0232600.1 Rorug03G0240500 Rorug04G0032900 Rorug04G0073300 Rorug04G0118500 Rorug04G0149600.1 Rorug04G0205100 Rorug04G0256700 Rorug05G0020200 Rorug05G0050000 Rorug05G0247300 Rorug05G0295100 Rorug05G0337900 Rorug05G0542600 Rorug06G0034800 Rorug06G0035100 Rorug06G0220100 Rorug07G0091900 Rorug07G0271200
rosa_samantha Rh1AG118200 Rh1AG253000 Rh1AG296300 Rh1AG409000 Rh1AG410600 Rh1BG073400 Rh1BG130400 Rh1BG147300 Rh1BG163600 Rh1CG016000 Rh1DG012200 Rh1DG012300 Rh1DG078600 Rh1DG078800 Rh1DG138100 Rh1DG179200 Rh1DG192600 Rh1DG194500 Rh1DG194600 Rh1DG214300 Rh1DG245900 Rh1DG267000 Rh1DG322700 Rh1DG442700 Rh2AG282000 Rh2AG423000 Rh2AG423100 Rh2AG460700 Rh2AG466200 Rh2AG513400 Rh2AG547000 Rh2BG277800 Rh2BG281200 Rh2BG298300 Rh2BG304300 Rh2BG433500 Rh2CG276000 Rh2CG282600 Rh2CG325500 Rh2CG393000 Rh2DG319200 Rh2DG443400 Rh3AG169300 Rh3AG303600 Rh3BG195000 Rh3BG282600 Rh3BG343400 Rh3BG343500 Rh3BG344100 Rh3CG111700 Rh3CG185700 Rh3CG259100 Rh3CG293000 Rh3CG317900 Rh3CG340900 Rh3CG341000 Rh3CG341100 Rh3CG354700 Rh3CG359200 Rh3CG368100 Rh3DG179900 Rh3DG288700 Rh3DG302600 Rh3DG316000 Rh3DG324500 Rh3DG324600 Rh3DG343200 Rh3DG343300 Rh3DG362400 Rh4AG071800 Rh4BG034000 Rh4BG044300 Rh4BG044400 Rh4BG069600 Rh4BG157400 Rh4CG007000 Rh4CG030400 Rh4CG037900 Rh4CG077700 Rh4CG115000 Rh4CG162400 Rh4CG162500 Rh4CG165500 Rh4CG165600 Rh4CG172900 Rh4CG239700 Rh4DG055900 Rh4DG173200 Rh5AG354900 Rh5AG511100 Rh5BG112600 Rh5BG541000 Rh5BG541100 Rh5BG549300 Rh5CG004400 Rh5CG315000 Rh5CG563800 Rh5CG572100 Rh5DG112200 Rh5DG191300 Rh5DG407600 Rh5DG407700 Rh5DG567500 Rh6AG002600 Rh6AG051400 Rh6AG199800 Rh6AG199900 Rh6AG206200 Rh6AG391000 Rh6AG391100 Rh6BG007500 Rh6BG053400 Rh6BG225900 Rh6BG227100 Rh6BG232400 Rh6CG091100 Rh6CG125400 Rh6CG186700 Rh6CG222700 Rh6CG222800 Rh6CG235500 Rh6CG247200 Rh6CG331200 Rh6CG465100 Rh6DG183500 Rh6DG213500 Rh6DG238800 Rh6DG263700 Rh7AG211800 Rh7AG441800 Rh7BG008600 Rh7BG253000 Rh7BG390000 Rh7CG189600 Rh7CG401500 Rh7CG424200 Rh7CG428700 Rh7CG479800 Rh7CG494300 Rh7DG177000 Rh7DG218700 Rh7DG254000 Rh7DG294800 Rh7DG407200 Rh7DG448800 Rh7DG461500
rosa_wichuraiana Rw0G012180 Rw1G005270 Rw1G012310 Rw1G016890 Rw1G021700 Rw2G014380 Rw2G027690 Rw3G016400 Rw3G028230 Rw3G029320 Rw4G002060 Rw4G002100 Rw4G003980 Rw4G014140 Rw4G014770 Rw4G014780 Rw4G015760 Rw4G021230 Rw5G000520 Rw5G035610 Rw6G001680 Rw6G017890 Rw6G027440 Rw6G034130 Rw6G034170 Rw7G012430 Rw7G033870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 217
Acc36I ACCTGC 1 cut(s) 217
Acc65I GGTACC 2 cut(s) 100, 833
AccB1I GGYRCC 2 cut(s) 100, 833
AccB7I CCANNNNNTGG 1 cut(s) 899
AccI GTMKAC 1 cut(s) 858
AccII CGCG 1 cut(s) 2373
AciI CCGC 3 cut(s) 2112, 2390, 2399
AclWI GGATC 4 cut(s) 1607, 2357, 2359, 2372
AcoI YGGCCR 3 cut(s) 598, 728, 2113
AcsI RAATTY 4 cut(s) 622, 918, 925, 1646
AcuI CTGAAG 2 cut(s) 1028, 1174
AcvI CACGTG 1 cut(s) 1520
AdeI CACNNNGTG 1 cut(s) 1555
AfaI GTAC 6 cut(s) 102, 778, 835, 1014, 1235, 1364
AgeI ACCGGT 1 cut(s) 985
AjnI CCWGG 7 cut(s) 26, 140, 242, 836, 1827, 2430, 2491
Alw21I GWGCWC 1 cut(s) 2429
Alw26I GTCTC 7 cut(s) 73, 328, 703, 876, 1838, 1979, 2492
AlwI GGATC 4 cut(s) 1607, 2357, 2359, 2372
AlwNI CAGNNNCTG 3 cut(s) 809, 1148, 1828
ApaI GGGCCC 1 cut(s) 2499
ApeKI GCWGC 2 cut(s) 970, 2396
ApoI RAATTY 4 cut(s) 622, 918, 925, 1646
ArsI GACNNNNNNTTYG 2 cut(s) 1056, 1088
AseI ATTAAT 1 cut(s) 2043
AsiGI ACCGGT 1 cut(s) 985
Asp718I GGTACC 2 cut(s) 100, 833
AsuC2I CCSGG 4 cut(s) 346, 1469, 1701, 2276
AsuII TTCGAA 1 cut(s) 1595
AvaII GGWCC 3 cut(s) 674, 812, 1456
AxyI CCTNAGG 2 cut(s) 461, 1158
BaeGI GKGCMC 1 cut(s) 2499
BalI TGGCCA 1 cut(s) 600
BamHI GGATCC 1 cut(s) 2364
BanI GGYRCC 2 cut(s) 100, 833
BanII GRGCYC 1 cut(s) 2499
BbrPI CACGTG 1 cut(s) 1520
BbsI GAAGAC 3 cut(s) 429, 692, 2339
Bbv12I GWGCWC 1 cut(s) 2429
BbvCI CCTCAGC 2 cut(s) 2175, 2417
BbvI GCAGC 2 cut(s) 957, 2408
BccI CCATC 9 cut(s) 227, 641, 808, 893, 1316, 1354, 1707, 2045, 2477
BciT130I CCWGG 7 cut(s) 28, 142, 244, 838, 1829, 2432, 2493
BciVI GTATCC 1 cut(s) 1946
BclI TGATCA 2 cut(s) 1258, 1740
BcnI CCSGG 4 cut(s) 346, 1469, 1701, 2276
BcoDI GTCTC 7 cut(s) 73, 328, 703, 876, 1838, 1979, 2492
BfaI CTAG 3 cut(s) 1026, 1083, 2502
BfmI CTRYAG 3 cut(s) 210, 855, 2157
BfuAI ACCTGC 1 cut(s) 217
BfuI GTATCC 1 cut(s) 1946
BisI GCNGC 4 cut(s) 971, 2113, 2397, 2400
BlsI GCNGC 4 cut(s) 972, 2114, 2398, 2401
Bme18I GGWCC 3 cut(s) 674, 812, 1456
BmrI ACTGGG 2 cut(s) 1214, 2446
BmsI GCATC 5 cut(s) 205, 212, 430, 718, 2383
BmuI ACTGGG 2 cut(s) 1214, 2446
BpiI GAAGAC 3 cut(s) 429, 692, 2339
BpmI CTGGAG 5 cut(s) 10, 163, 662, 2148, 2180
Bpu10I CCTNAGC 2 cut(s) 2175, 2417
Bpu14I TTCGAA 1 cut(s) 1595
BpuMI CCSGG 4 cut(s) 346, 1469, 1701, 2276
BsaAI YACGTR 1 cut(s) 1520
BsaI GGTCTC 4 cut(s) 73, 328, 1838, 2492
BsaJI CCNNGG 9 cut(s) 242, 677, 829, 1118, 1467, 1699, 2430, 2491, 2492
BsaWI WCCGGW 2 cut(s) 985, 1567
BsaXI ACNNNNNCTCC 6 cut(s) 8, 38, 176, 206, 896, 926
Bse118I RCCGGY 3 cut(s) 726, 985, 1538
Bse1I ACTGG 4 cut(s) 508, 1040, 1209, 2441
Bse21I CCTNAGG 2 cut(s) 461, 1158
Bse3DI GCAATG 1 cut(s) 1897
BseBI CCWGG 7 cut(s) 28, 142, 244, 838, 1829, 2432, 2493
BseDI CCNNGG 9 cut(s) 242, 677, 829, 1118, 1467, 1699, 2430, 2491, 2492
BseGI GGATG 8 cut(s) 174, 652, 687, 1308, 1396, 2056, 2068, 2398
BseMI GCAATG 1 cut(s) 1897
BseMII CTCAG 9 cut(s) 452, 1149, 1155, 1566, 1953, 2027, 2166, 2196, 2408
BseNI ACTGG 4 cut(s) 508, 1040, 1209, 2441
BseRI GAGGAG 2 cut(s) 1278, 1548
BseSI GKGCMC 1 cut(s) 2499
BseXI GCAGC 2 cut(s) 957, 2408
Bsh1236I CGCG 1 cut(s) 2373
BshNI GGYRCC 2 cut(s) 100, 833
BshTI ACCGGT 1 cut(s) 985
BsiHKAI GWGCWC 1 cut(s) 2429
BsiSI CCGG 8 cut(s) 345, 727, 986, 1469, 1539, 1568, 1701, 2276
BslFI GGGAC 2 cut(s) 1469, 2288
BsmAI GTCTC 7 cut(s) 73, 328, 703, 876, 1838, 1979, 2492
BsmBI CGTCTC 1 cut(s) 703
BsmFI GGGAC 2 cut(s) 1469, 2288
BsmI GAATGC 1 cut(s) 382
Bso31I GGTCTC 4 cut(s) 73, 328, 1838, 2492
Bsp119I TTCGAA 1 cut(s) 1595
Bsp120I GGGCCC 1 cut(s) 2495
Bsp1286I GDGCHC 2 cut(s) 2429, 2499
Bsp143I GATC 8 cut(s) 387, 882, 1258, 1612, 1740, 1908, 2349, 2364
Bsp19I CCATGG 2 cut(s) 677, 1118
Bsp68I TCGCGA 1 cut(s) 2373
BspACI CCGC 3 cut(s) 2112, 2390, 2399
BspCNI CTCAG 9 cut(s) 453, 1150, 1154, 1565, 1952, 2026, 2167, 2197, 2409
BspFNI CGCG 1 cut(s) 2373
BspHI TCATGA 1 cut(s) 2211
BspMAI CTGCAG 2 cut(s) 214, 2161
BspMI ACCTGC 1 cut(s) 217
BspPI GGATC 4 cut(s) 1607, 2357, 2359, 2372
BspT104I TTCGAA 1 cut(s) 1595
BspT107I GGYRCC 2 cut(s) 100, 833
BspTNI GGTCTC 4 cut(s) 73, 328, 1838, 2492
BsrDI GCAATG 1 cut(s) 1897
BsrFI RCCGGY 3 cut(s) 726, 985, 1538
BsrI ACTGG 4 cut(s) 508, 1040, 1209, 2441
BssAI RCCGGY 3 cut(s) 726, 985, 1538
BssECI CCNNGG 9 cut(s) 242, 677, 829, 1118, 1467, 1699, 2430, 2491, 2492
BssMI GATC 8 cut(s) 387, 882, 1258, 1612, 1740, 1908, 2349, 2364
BssT1I CCWWGG 3 cut(s) 677, 829, 1118
Bst2UI CCWGG 7 cut(s) 28, 142, 244, 838, 1829, 2432, 2493
Bst4CI ACNGT 4 cut(s) 409, 1238, 1362, 2384
Bst6I CTCTTC 2 cut(s) 1081, 2008
BstAPI GCANNNNNTGC 2 cut(s) 89, 209
BstBAI YACGTR 1 cut(s) 1520
BstBI TTCGAA 1 cut(s) 1595
BstC8I GCNNGC 2 cut(s) 1997, 2202
BstDSI CCRYGG 2 cut(s) 677, 1118
BstEII GGTNACC 2 cut(s) 988, 1570
BstENI CCTNNNNNAGG 1 cut(s) 142
BstF5I GGATG 8 cut(s) 174, 652, 687, 1308, 1396, 2056, 2068, 2398
BstFNI CGCG 1 cut(s) 2373
BstKTI GATC 8 cut(s) 390, 885, 1261, 1615, 1743, 1911, 2352, 2367
BstMAI GTCTC 7 cut(s) 73, 328, 703, 876, 1838, 1979, 2492
BstMBI GATC 8 cut(s) 387, 882, 1258, 1612, 1740, 1908, 2349, 2364
BstMWI GCNNNNNNNGC 7 cut(s) 89, 209, 727, 1512, 1868, 2396, 2408
BstNI CCWGG 7 cut(s) 28, 142, 244, 838, 1829, 2432, 2493
BstNSI RCATGY 1 cut(s) 251
BstPI GGTNACC 2 cut(s) 988, 1570
BstSFI CTRYAG 3 cut(s) 210, 855, 2157
BstSLI GKGCMC 1 cut(s) 2499
BstUI CGCG 1 cut(s) 2373
BstV1I GCAGC 2 cut(s) 957, 2408
BstV2I GAAGAC 3 cut(s) 429, 692, 2339
BstX2I RGATCY 1 cut(s) 2364
BstYI RGATCY 1 cut(s) 2364
Bsu36I CCTNAGG 2 cut(s) 461, 1158
BsuI GTATCC 1 cut(s) 1946
BtgI CCRYGG 2 cut(s) 677, 1118
BtsCI GGATG 8 cut(s) 174, 652, 687, 1308, 1396, 2056, 2068, 2398
BtsIMutI CAGTG 4 cut(s) 307, 810, 1149, 1560
BtuMI TCGCGA 1 cut(s) 2373
BveI ACCTGC 1 cut(s) 217
Cac8I GCNNGC 2 cut(s) 1997, 2202
CaiI CAGNNNCTG 3 cut(s) 809, 1148, 1828
CciI TCATGA 1 cut(s) 2211
Cfr10I RCCGGY 3 cut(s) 726, 985, 1538
Csp6I GTAC 6 cut(s) 101, 777, 834, 1013, 1234, 1363
CspAI ACCGGT 1 cut(s) 985
CspCI CAANNNNNGTGG 2 cut(s) 147, 182
CviQI GTAC 6 cut(s) 101, 777, 834, 1013, 1234, 1363
DpnI GATC 8 cut(s) 389, 884, 1260, 1614, 1742, 1910, 2351, 2366
DpnII GATC 8 cut(s) 387, 882, 1258, 1612, 1740, 1908, 2349, 2364
DraIII CACNNNGTG 1 cut(s) 1555
EaeI YGGCCR 3 cut(s) 598, 728, 2113
Eam1104I CTCTTC 2 cut(s) 1081, 2008
EarI CTCTTC 2 cut(s) 1081, 2008
Eco130I CCWWGG 3 cut(s) 677, 829, 1118
Eco147I AGGCCT 2 cut(s) 1195, 2449
Eco24I GRGCYC 1 cut(s) 2499
Eco31I GGTCTC 4 cut(s) 73, 328, 1838, 2492
Eco32I GATATC 1 cut(s) 342
Eco47I GGWCC 3 cut(s) 674, 812, 1456
Eco57I CTGAAG 2 cut(s) 1028, 1174
Eco72I CACGTG 1 cut(s) 1520
Eco81I CCTNAGG 2 cut(s) 461, 1158
Eco91I GGTNACC 2 cut(s) 988, 1570
EcoNI CCTNNNNNAGG 1 cut(s) 142
EcoO109I RGGNCCY 4 cut(s) 1456, 2191, 2495, 2496
EcoO65I GGTNACC 2 cut(s) 988, 1570
EcoRII CCWGG 7 cut(s) 26, 140, 242, 836, 1827, 2430, 2491
EcoRV GATATC 1 cut(s) 342
EcoT14I CCWWGG 3 cut(s) 677, 829, 1118
EcoT38I GRGCYC 1 cut(s) 2499
ErhI CCWWGG 3 cut(s) 677, 829, 1118
Esp3I CGTCTC 1 cut(s) 703
FalI AAGNNNNNCTT 2 cut(s) 1479, 1511
FaqI GGGAC 2 cut(s) 1469, 2288
FbaI TGATCA 2 cut(s) 1258, 1740
FblI GTMKAC 1 cut(s) 858
Fnu4HI GCNGC 4 cut(s) 971, 2113, 2397, 2400
FokI GGATG 8 cut(s) 161, 659, 694, 1295, 1383, 2063, 2075, 2405
FriOI GRGCYC 1 cut(s) 2499
Fsp4HI GCNGC 4 cut(s) 971, 2113, 2397, 2400
FspBI CTAG 3 cut(s) 1026, 1083, 2502
GluI GCNGC 4 cut(s) 971, 2113, 2397, 2400
GsuI CTGGAG 5 cut(s) 10, 163, 662, 2148, 2180
HapII CCGG 8 cut(s) 345, 727, 986, 1469, 1539, 1568, 1701, 2276
HpaII CCGG 8 cut(s) 345, 727, 986, 1469, 1539, 1568, 1701, 2276
Hpy166II GTNNAC 6 cut(s) 128, 653, 859, 1015, 1080, 2138
Hpy8I GTNNAC 6 cut(s) 128, 653, 859, 1015, 1080, 2138
HpyAV CCTTC 4 cut(s) 1003, 1491, 1626, 2471
HpyCH4III ACNGT 4 cut(s) 409, 1238, 1362, 2384
HpyCH4IV ACGT 4 cut(s) 1232, 1519, 1693, 1979
HpyCH4V TGCA 9 cut(s) 83, 92, 158, 212, 218, 618, 1995, 2159, 2396
HpyF10VI GCNNNNNNNGC 7 cut(s) 89, 209, 727, 1512, 1868, 2396, 2408
HpySE526I ACGT 4 cut(s) 1232, 1519, 1693, 1979
KflI GGGWCCC 1 cut(s) 1456
KpnI GGTACC 2 cut(s) 104, 837
Ksp22I TGATCA 2 cut(s) 1258, 1740
Kzo9I GATC 8 cut(s) 387, 882, 1258, 1612, 1740, 1908, 2349, 2364
LmnI GCTCC 5 cut(s) 978, 1535, 2054, 2129, 2432
Lsp1109I GCAGC 2 cut(s) 957, 2408
LweI GCATC 5 cut(s) 205, 212, 430, 718, 2383
MaeI CTAG 3 cut(s) 1026, 1083, 2502
MaeII ACGT 4 cut(s) 1232, 1519, 1693, 1979
MalI GATC 8 cut(s) 389, 884, 1260, 1614, 1742, 1910, 2351, 2366
MboI GATC 8 cut(s) 387, 882, 1258, 1612, 1740, 1908, 2349, 2364
MfeI CAATTG 3 cut(s) 1450, 1878, 2121
MflI RGATCY 1 cut(s) 2364
MhlI GDGCHC 2 cut(s) 2429, 2499
MlsI TGGCCA 1 cut(s) 600
MluNI TGGCCA 1 cut(s) 600
MlyI GAGTC 6 cut(s) 552, 1098, 1291, 1671, 1673, 2329
MmeI TCCRAC 3 cut(s) 12, 1962, 2477
Mox20I TGGCCA 1 cut(s) 600
MscI TGGCCA 1 cut(s) 600
MseI TTAA 6 cut(s) 741, 1281, 1323, 2043, 2313, 2474
MslI CAYNNNNRTG 1 cut(s) 609
Msp20I TGGCCA 1 cut(s) 600
MspA1I CMGCKG 3 cut(s) 896, 2200, 2399
MspI CCGG 8 cut(s) 345, 727, 986, 1469, 1539, 1568, 1701, 2276
MunI CAATTG 3 cut(s) 1450, 1878, 2121
Mva1269I GAATGC 1 cut(s) 382
MvaI CCWGG 7 cut(s) 28, 142, 244, 838, 1829, 2432, 2493
MvnI CGCG 1 cut(s) 2373
MwoI GCNNNNNNNGC 7 cut(s) 89, 209, 727, 1512, 1868, 2396, 2408
NciI CCSGG 4 cut(s) 346, 1469, 1701, 2276
NcoI CCATGG 2 cut(s) 677, 1118
NdeII GATC 8 cut(s) 387, 882, 1258, 1612, 1740, 1908, 2349, 2364
NmuCI GTSAC 6 cut(s) 769, 902, 988, 1051, 1131, 2320
NruI TCGCGA 1 cut(s) 2373
NspI RCATGY 1 cut(s) 251
NspV TTCGAA 1 cut(s) 1595
PagI TCATGA 1 cut(s) 2211
PaqCI CACCTGC 1 cut(s) 217
PasI CCCWGGG 1 cut(s) 2492
PceI AGGCCT 2 cut(s) 1195, 2449
PctI GAATGC 1 cut(s) 382
PfeI GAWTC 8 cut(s) 148, 264, 482, 630, 788, 1264, 1901, 2104
PflFI GACNNNGTC 1 cut(s) 691
PflMI CCANNNNNTGG 1 cut(s) 899
PfoI TCCNGGA 1 cut(s) 140
PinAI ACCGGT 1 cut(s) 985
PkrI GCNGC 4 cut(s) 972, 2114, 2398, 2401
PleI GAGTC 6 cut(s) 551, 1097, 1291, 1670, 1673, 2329
PmaCI CACGTG 1 cut(s) 1520
PmlI CACGTG 1 cut(s) 1520
PpsI GAGTC 6 cut(s) 551, 1097, 1291, 1670, 1673, 2329
Ppu21I YACGTR 1 cut(s) 1520
PpuMI RGGWCCY 1 cut(s) 1456
PshBI ATTAAT 1 cut(s) 2043
Psp5II RGGWCCY 1 cut(s) 1456
Psp6I CCWGG 7 cut(s) 26, 140, 242, 836, 1827, 2430, 2491
PspCI CACGTG 1 cut(s) 1520
PspEI GGTNACC 2 cut(s) 988, 1570
PspGI CCWGG 7 cut(s) 26, 140, 242, 836, 1827, 2430, 2491
PspOMI GGGCCC 1 cut(s) 2495
PspPPI RGGWCCY 1 cut(s) 1456
PstI CTGCAG 2 cut(s) 214, 2161
PstNI CAGNNNCTG 3 cut(s) 809, 1148, 1828
PsuI RGATCY 1 cut(s) 2364
PsyI GACNNNGTC 1 cut(s) 691
PvuII CAGCTG 2 cut(s) 896, 2200
RruI TCGCGA 1 cut(s) 2373
RsaI GTAC 6 cut(s) 102, 778, 835, 1014, 1235, 1364
RsaNI GTAC 6 cut(s) 101, 777, 834, 1013, 1234, 1363
RseI CAYNNNNRTG 1 cut(s) 609
SaqAI TTAA 6 cut(s) 741, 1281, 1323, 2043, 2313, 2474
SatI GCNGC 4 cut(s) 971, 2113, 2397, 2400
Sau3AI GATC 8 cut(s) 387, 882, 1258, 1612, 1740, 1908, 2349, 2364
SchI GAGTC 6 cut(s) 552, 1098, 1291, 1671, 1673, 2329
SduI GDGCHC 2 cut(s) 2429, 2499
SfaNI GCATC 5 cut(s) 205, 212, 430, 718, 2383
SfcI CTRYAG 3 cut(s) 210, 855, 2157
SfuI TTCGAA 1 cut(s) 1595
SgrAI CRCCGGYG 1 cut(s) 985
SinI GGWCC 3 cut(s) 674, 812, 1456
SmiMI CAYNNNNRTG 1 cut(s) 609
SseBI AGGCCT 2 cut(s) 1195, 2449
SsiI CCGC 3 cut(s) 2112, 2390, 2399
SspMI CTAG 3 cut(s) 1026, 1083, 2502
StuI AGGCCT 2 cut(s) 1195, 2449
StyI CCWWGG 3 cut(s) 677, 829, 1118
TaaI ACNGT 4 cut(s) 409, 1238, 1362, 2384
TaiI ACGT 4 cut(s) 1235, 1522, 1696, 1982
TaqI TCGA 5 cut(s) 546, 864, 1074, 1595, 1665
TatI WGTACW 1 cut(s) 1362
TauI GCSGC 2 cut(s) 2115, 2402
TfiI GAWTC 8 cut(s) 148, 264, 482, 630, 788, 1264, 1901, 2104
Tru1I TTAA 6 cut(s) 741, 1281, 1323, 2043, 2313, 2474
Tru9I TTAA 6 cut(s) 741, 1281, 1323, 2043, 2313, 2474
TscAI CASTG 4 cut(s) 307, 810, 1149, 1560
TseFI GTSAC 6 cut(s) 769, 902, 988, 1051, 1131, 2320
TseI GCWGC 2 cut(s) 970, 2396
Tsp45I GTSAC 6 cut(s) 769, 902, 988, 1051, 1131, 2320
TspDTI ATGAA 7 cut(s) 299, 380, 540, 698, 1911, 2200, 2228
TspGWI ACGGA 1 cut(s) 685
TspRI CASTG 4 cut(s) 307, 810, 1149, 1560
Tth111I GACNNNGTC 1 cut(s) 691
Van91I CCANNNNNTGG 1 cut(s) 899
VpaK11BI GGWCC 3 cut(s) 674, 812, 1456
VspI ATTAAT 1 cut(s) 2043
XagI CCTNNNNNAGG 1 cut(s) 142
XapI RAATTY 4 cut(s) 622, 918, 925, 1646
XceI RCATGY 1 cut(s) 251
XcmI CCANNNNNNNNNTGG 4 cut(s) 507, 663, 1925, 2123
XmiI GTMKAC 1 cut(s) 858
XspI CTAG 3 cut(s) 1026, 1083, 2502
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.