Rh5BG541100

Serine/Threonine protein kinases, catalytic domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000073
Physical Location & Seq
Forward (+)
85755669 .. 85762995
7327 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG541100.1

Sequence Viewer

Length: 1689 bp
ATGGCTGCTCCGCCCCTTTGTTGCCTTCTTGCTGACAACCCCCGAGGCTGGGATCGGCCTCCGAATCAATACTTTGAGGGCCGCTGGAATCGATATGTTGAGCGCCGTAATAATCAAGATGGAAATTACCGTTCCCGACCATGGAACCCTTTGCCTGTGACGGGAGCCCTAGTTCTTGCCCATCCCCAACAGGGCAGGTGGCAAGATGTGCCAATTAATCCACCTATATCTAATGCCCCTGTGGTCACCTCAGCCGAAGCAGGTCCTTCCCACTCCAAATTCATTGTTAAGGGCAAAGCGATTGTTGAAGATTGGCTCCACCCACTCACACCACCTTCGCCAGATCATCCTATGTCTCCTGACGTGGATGATATGAAGTTGGCTATATCTCTCTCACTAGCTGACGTGGTGAATCCTTCTCAACACTTCGGTCCTCATCCTGATGATTCGGGTTCTAATGTCATGCAGCATATGGGCCCAACTATAGCTCCTGCTTTACTCCAGTCAGCTTTGGCCCATCAAGAAATGCTTTTGGATCCAAATCTTCTTTACCCAACCCCACCTTCTATTCCTGCGACCCATGCTAATCCGATGCTACTTGATCCGACCCTCCCAACCTTAATCCCTAATTTCACTCACCCTGTTCCTTTTTATGATCCCCTTCTACCAACTGCTGTTCCCGCTTTCGTCCCAACCCCAGATCCTCTTCACCCAGGGTTTTATGGGGGACCTTCTCAATCACTTCCTCCCTTAGGATCCTTGGCTACCTTAGCTCATCAATTCCAACATCTTCATGTTCGTAAGCGTCTGGAGGATGCTTTGGCTGAGACAACTAGGCAGCTGAAAAAATACAAGGCAATGGAATCACAGGAACCAGAGAGCCTCCCACCCACCTCTCACAAAATCACTGCTTCCAAAGCCAAAATGGGGAAATCAACTCTTTCAGTTTCGGTTAGGGGCAGCGGTAGCCGGGGCCATCCTCGTAAAGCCGCATCTACACTGGCTATCCCACCATCCTCTACTACTGATTTCCAGGTTAGTGGTGGCATCTCTTCATCACTTTCAACTATTCCGGCACCTCCTCTCCACTCTGGGTTTGGTAATGGTCAGGCTGTGTCTTCAGTTGAGATGGTTTCTATTCCAATGGTTATGGGTACTTCTACTGTTGCACTACCAAGCATTTCGGCTTCTCTTGTTTCATCCACTGCTGATATAGGCATAGTTGACGACTACTTGAAGATTTGGAAGGTTTGGGAAAGTTCTGGAAGTGCATTGTCGCATGCTGATTGTTGCAAGTTTTGGAGTTATATCTCGAAGCACTGGAATTCAAAGACAGAAAAGACTGTTGCTGATGCCTTGGTGAAAGTACCTGTCAGTTCTGGATCTGAAGGAATCTTGCTGTGCAACAAGTCTGATGTTTTCATTGCTGATGACCTTCAGCTGAAAATTGGTGTTCGCACTATATCTGAATCTGTACAGAAGGAAGAGGCGTCACTTGCAGATGATGTTGAAGCAGTCTCTTTGCCGAGGGAAAAGCTGATTAAGAAAGCGCTGTTCAGGCTGATTCTTGGTTTTCTTGCTACTCCTCCCATGGAAATGGAAGTTGAGAAGAGGCTTGAAGCTGTTCAATTCCTTGTGAATATGGAACTCACGGAACAATTGGAAAAGGCAAATAGAATAAGGACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

562

Amino Acids

60.97

Weight (kDa)

6.44

Isoelectric Point (pI)

41.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000073)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g17821 FvH4_2g04610 FvH4_2g09611 FvH4_2g13563 FvH4_3g21493 FvH4_3g32115 FvH4_3g35060 FvH4_4g00220 FvH4_4g06410 FvH4_4g10367 FvH4_4g10367 FvH4_4g10367 FvH4_5g06101 FvH4_5g08842 FvH4_5g27366 FvH4_5g31283 FvH4_6g11611 FvH4_6g23032 FvH4_7g05661 FvH4_7g06172
malus_domestica MD13G1263700.v1.1
prunus_persica Prupe.1G292300_v2.0.a1 Prupe.5G009700_v2.0.a1 Prupe.6G158100_v2.0.a1 Prupe.8G025100_v2.0.a1 Prupe.8G042600_v2.0.a1
pyrus_communis pycom02g02250 pycom02g16030 pycom05g10490 pycom09g06450 pycom09g06460 pycom13g26200 pycom16g17700
rosa_chinensis RchiOBHm_Chr2g0163321 RchiOBHm_Chr4g0401981 RchiOBHm_Chr5g0005991 RchiOBHm_Chr5g0027191 RchiOBHm_Chr6g0249771
rosa_laevigata RLG00000001040 RLG00000001764 RLG00000002239 RLG00000002240 RLG00000003259 RLG00000008993 RLG00000013847 RLG00000014178 RLG00000015576 RLG00000017269 RLG00000017355 RLG00000018127 RLG00000018602 RLG00000018615 RLG00000019904 RLG00000020044 RLG00000021849 RLG00000021850 RLG00000027965 RLG00000029266 RLG00000029820 RLG00000031112 RLG00000031915 RLG00000033568 RLG00000035013 RLG00000035677 RLG00000036568 RLG00000036628
rosa_multiflora Rmu_co8254085.1_g000001 Rmu_co8462383.1_g000001 Rmu_sc0000185.1_g000049 Rmu_sc0000251.1_g000018 Rmu_sc0000367.1_g000094 Rmu_sc0000498.1_g000023 Rmu_sc0000868.1_g000005 Rmu_sc0001521.1_g000047 Rmu_sc0001534.1_g000007 Rmu_sc0002014.1_g000012 Rmu_sc0003214.1_g000009 Rmu_sc0003573.1_g000041 Rmu_sc0003832.1_g000023 Rmu_sc0004677.1_g000015 Rmu_sc0004967.1_g000014 Rmu_sc0005426.1_g000001 Rmu_sc0005621.1_g000004 Rmu_sc0005640.1_g000009 Rmu_sc0005947.1_g000034 Rmu_sc0006889.1_g000011 Rmu_sc0007121.1_g000014 Rmu_sc0009191.1_g000017 Rmu_sc0010556.1_g000011 Rmu_sc0011396.1_g000007 Rmu_sc0019862.1_g000002 Rmu_sc0022931.1_g000003 Rmu_sc0024164.1_g000001 Rmu_ssc0000391.1_g000027
rosa_roxburghii Rroxscaffold_1G00050060 Rroxscaffold_1G00061810 Rroxscaffold_2G00109590 Rroxscaffold_2G00113810 Rroxscaffold_2G00124040 Rroxscaffold_2G00143620 Rroxscaffold_2G00154760 Rroxscaffold_4G00302820 Rroxscaffold_4G00302830 Rroxscaffold_4G00308680 Rroxscaffold_4G00310240 Rroxscaffold_4G00327890 Rroxscaffold_5G00334600 Rroxscaffold_5G00336050 Rroxscaffold_5G00346560 Rroxscaffold_5G00347860 Rroxscaffold_5G00347870 Rroxscaffold_5G00349330 Rroxscaffold_5G00351980 Rroxscaffold_6G00395280 Rroxscaffold_6G00396750 Rroxscaffold_6G00402910 Rroxscaffold_7G00174790 Rroxscaffold_7G00177570 Rroxscaffold_7G00191820 Rroxscaffold_7G00203480 Rroxscaffold_7G00204380
rosa_rugosa Rorug01G0106600 Rorug01G0106600 Rorug01G0148500.1 Rorug02G0224200 Rorug02G0225500 Rorug02G0233000 Rorug02G0630400 Rorug03G0198000 Rorug03G0232600.1 Rorug03G0240500 Rorug04G0032900 Rorug04G0073300 Rorug04G0118500 Rorug04G0149600.1 Rorug04G0205100 Rorug04G0256700 Rorug05G0020200 Rorug05G0050000 Rorug05G0247300 Rorug05G0295100 Rorug05G0337900 Rorug05G0542600 Rorug06G0034800 Rorug06G0035100 Rorug06G0220100 Rorug07G0091900 Rorug07G0271200
rosa_samantha Rh1AG118200 Rh1AG253000 Rh1AG296300 Rh1AG409000 Rh1AG410600 Rh1BG073400 Rh1BG130400 Rh1BG147300 Rh1BG163600 Rh1CG016000 Rh1DG012200 Rh1DG012300 Rh1DG078600 Rh1DG078800 Rh1DG138100 Rh1DG179200 Rh1DG192600 Rh1DG194500 Rh1DG194600 Rh1DG214300 Rh1DG245900 Rh1DG267000 Rh1DG322700 Rh1DG442700 Rh2AG282000 Rh2AG423000 Rh2AG423100 Rh2AG460700 Rh2AG466200 Rh2AG513400 Rh2AG547000 Rh2BG277800 Rh2BG281200 Rh2BG298300 Rh2BG304300 Rh2BG433500 Rh2CG276000 Rh2CG282600 Rh2CG325500 Rh2CG393000 Rh2DG319200 Rh2DG443400 Rh3AG169300 Rh3AG303600 Rh3BG195000 Rh3BG282600 Rh3BG343400 Rh3BG343500 Rh3BG344100 Rh3CG111700 Rh3CG185700 Rh3CG259100 Rh3CG293000 Rh3CG317900 Rh3CG340900 Rh3CG341000 Rh3CG341100 Rh3CG354700 Rh3CG359200 Rh3CG368100 Rh3DG179900 Rh3DG288700 Rh3DG302600 Rh3DG316000 Rh3DG324500 Rh3DG324600 Rh3DG343200 Rh3DG343300 Rh3DG362400 Rh4AG071800 Rh4BG034000 Rh4BG044300 Rh4BG044400 Rh4BG069600 Rh4BG157400 Rh4CG007000 Rh4CG030400 Rh4CG037900 Rh4CG077700 Rh4CG115000 Rh4CG162400 Rh4CG162500 Rh4CG165500 Rh4CG165600 Rh4CG172900 Rh4CG239700 Rh4DG055900 Rh4DG173200 Rh5AG354900 Rh5AG511100 Rh5BG112600 Rh5BG541000 Rh5BG541100 Rh5BG549300 Rh5CG004400 Rh5CG315000 Rh5CG563800 Rh5CG572100 Rh5DG112200 Rh5DG191300 Rh5DG407600 Rh5DG407700 Rh5DG567500 Rh6AG002600 Rh6AG051400 Rh6AG199800 Rh6AG199900 Rh6AG206200 Rh6AG391000 Rh6AG391100 Rh6BG007500 Rh6BG053400 Rh6BG225900 Rh6BG227100 Rh6BG232400 Rh6CG091100 Rh6CG125400 Rh6CG186700 Rh6CG222700 Rh6CG222800 Rh6CG235500 Rh6CG247200 Rh6CG331200 Rh6CG465100 Rh6DG183500 Rh6DG213500 Rh6DG238800 Rh6DG263700 Rh7AG211800 Rh7AG441800 Rh7BG008600 Rh7BG253000 Rh7BG390000 Rh7CG189600 Rh7CG401500 Rh7CG424200 Rh7CG428700 Rh7CG479800 Rh7CG494300 Rh7DG177000 Rh7DG218700 Rh7DG254000 Rh7DG294800 Rh7DG407200 Rh7DG448800 Rh7DG461500
rosa_wichuraiana Rw0G012180 Rw1G005270 Rw1G012310 Rw1G016890 Rw1G021700 Rw2G014380 Rw2G027690 Rw3G016400 Rw3G028230 Rw3G029320 Rw4G002060 Rw4G002100 Rw4G003980 Rw4G014140 Rw4G014770 Rw4G014780 Rw4G015760 Rw4G021230 Rw5G000520 Rw5G035610 Rw6G001680 Rw6G017890 Rw6G027440 Rw6G034130 Rw6G034170 Rw7G012430 Rw7G033870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 186
Acc36I ACCTGC 2 cut(s) 186, 251
AccB1I GGYRCC 1 cut(s) 1075
AciI CCGC 5 cut(s) 11, 82, 681, 963, 990
AclWI GGATC 9 cut(s) 60, 530, 543, 596, 650, 695, 750, 763, 1390
AcsI RAATTY 2 cut(s) 278, 1324
AcuI CTGAAG 3 cut(s) 1104, 1407, 1421
AcyI GRCGYC 1 cut(s) 1490
AfaI GTAC 3 cut(s) 1156, 1368, 1476
AfeI AGCGCT 1 cut(s) 1551
AfiI CCNNNNNNNGG 7 cut(s) 48, 49, 141, 161, 191, 752, 927
AgsI TTSAA 7 cut(s) 308, 1065, 1237, 1329, 1511, 1619, 1628
AjiI CACGTC 2 cut(s) 364, 406
AjnI CCWGG 2 cut(s) 712, 1032
AjuI GAANNNNNNNTTGG 2 cut(s) 547, 579
AloI GAACNNNNNNTCC 2 cut(s) 156, 188
AluBI AGCT 8 cut(s) 401, 488, 509, 773, 841, 1441, 1537, 1622
AluI AGCT 8 cut(s) 401, 488, 509, 773, 841, 1441, 1537, 1622
Alw26I GTCTC 3 cut(s) 360, 821, 1522
AlwI GGATC 9 cut(s) 60, 530, 543, 596, 650, 695, 750, 763, 1390
Ama87I CYCGRG 1 cut(s) 42
Aor51HI AGCGCT 1 cut(s) 1551
AoxI GGCC 5 cut(s) 56, 79, 475, 513, 973
ApaI GGGCCC 1 cut(s) 479
ApeKI GCWGC 4 cut(s) 5, 466, 838, 960
ApoI RAATTY 2 cut(s) 278, 1324
AseI ATTAAT 1 cut(s) 216
Asp700I GAANNNNTTC 1 cut(s) 1623
AspLEI GCGC 2 cut(s) 105, 1552
AspS9I GGNCC 9 cut(s) 79, 263, 431, 475, 476, 514, 728, 973, 1683
AsuC2I CCSGG 1 cut(s) 971
AsuHPI GGTGA 5 cut(s) 238, 421, 629, 701, 1372
AvaI CYCGRG 1 cut(s) 42
AvaII GGWCC 4 cut(s) 263, 431, 728, 1683
AxyI CCTNAGG 1 cut(s) 751
BaeGI GKGCMC 1 cut(s) 479
BamHI GGATCC 2 cut(s) 535, 755
BanI GGYRCC 1 cut(s) 1075
BanII GRGCYC 2 cut(s) 169, 479
BbsI GAAGAC 1 cut(s) 1110
BbvCI CCTCAGC 1 cut(s) 250
BbvI GCAGC 3 cut(s) 478, 850, 972
BccI CCATC 6 cut(s) 113, 189, 525, 984, 1021, 1123
BceAI ACGGC 1 cut(s) 90
BciT130I CCWGG 2 cut(s) 714, 1034
BcnI CCSGG 1 cut(s) 971
BcoDI GTCTC 3 cut(s) 360, 821, 1522
BfaI CTAG 3 cut(s) 170, 398, 834
BfmI CTRYAG 1 cut(s) 483
BfoI RGCGCY 2 cut(s) 106, 1553
BfuAI ACCTGC 2 cut(s) 186, 251
BisI GCNGC 6 cut(s) 6, 82, 467, 839, 961, 990
BlsI GCNGC 6 cut(s) 7, 83, 468, 840, 962, 991
Bme1390I CCNGG 3 cut(s) 714, 971, 1034
Bme18I GGWCC 4 cut(s) 263, 431, 728, 1683
BmeT110I CYCGRG 1 cut(s) 42
BmgBI CACGTC 2 cut(s) 364, 406
BmgT120I GGNCC 9 cut(s) 79, 263, 431, 475, 476, 514, 728, 973, 1683
BmrFI CCNGG 3 cut(s) 714, 971, 1034
BmsI GCATC 5 cut(s) 582, 805, 1001, 1056, 1342
BpiI GAAGAC 1 cut(s) 1110
BpmI CTGGAG 2 cut(s) 485, 830
Bpu10I CCTNAGC 2 cut(s) 250, 769
BpuMI CCSGG 1 cut(s) 971
Bsa29I ATCGAT 1 cut(s) 91
BsaBI GATNNNNATC 1 cut(s) 540
BsaHI GRCGYC 1 cut(s) 1490
BsaJI CCNNGG 9 cut(s) 43, 140, 712, 713, 759, 970, 1356, 1526, 1590
BsaXI ACNNNNNCTCC 6 cut(s) 156, 186, 472, 502, 1068, 1098
Bsc4I CCNNNNNNNGG 7 cut(s) 48, 49, 141, 161, 191, 752, 927
Bse1I ACTGG 3 cut(s) 502, 1005, 1325
Bse21I CCTNAGG 1 cut(s) 751
Bse3DI GCAATG 2 cut(s) 864, 1422
Bse8I GATNNNNATC 1 cut(s) 540
BseBI CCWGG 2 cut(s) 714, 1034
BseCI ATCGAT 1 cut(s) 91
BseDI CCNNGG 9 cut(s) 43, 140, 712, 713, 759, 970, 1356, 1526, 1590
BseGI GGATG 8 cut(s) 181, 346, 373, 436, 820, 976, 1013, 1199
BseJI GATNNNNATC 1 cut(s) 540
BseLI CCNNNNNNNGG 7 cut(s) 48, 49, 141, 161, 191, 752, 927
BseMI GCAATG 2 cut(s) 864, 1422
BseMII CTCAG 2 cut(s) 264, 816
BseNI ACTGG 3 cut(s) 502, 1005, 1325
BseRI GAGGAG 2 cut(s) 1071, 1575
BseSI GKGCMC 1 cut(s) 479
BseXI GCAGC 3 cut(s) 478, 850, 972
BseYI CCCAGC 1 cut(s) 48
BshFI GGCC 5 cut(s) 58, 81, 477, 515, 975
BshNI GGYRCC 1 cut(s) 1075
BshVI ATCGAT 1 cut(s) 91
BsiHKCI CYCGRG 1 cut(s) 42
BsiSI CCGG 2 cut(s) 970, 1073
BslFI GGGAC 2 cut(s) 674, 741
BslI CCNNNNNNNGG 7 cut(s) 48, 49, 141, 161, 191, 752, 927
BsmAI GTCTC 3 cut(s) 360, 821, 1522
BsmFI GGGAC 2 cut(s) 674, 741
BsnI GGCC 5 cut(s) 58, 81, 477, 515, 975
BsoBI CYCGRG 1 cut(s) 42
Bsp120I GGGCCC 1 cut(s) 475
Bsp1286I GDGCHC 2 cut(s) 169, 479
Bsp1407I TGTACA 1 cut(s) 1474
Bsp143I GATC 8 cut(s) 52, 343, 535, 601, 655, 700, 755, 1382
Bsp19I CCATGG 2 cut(s) 140, 1590
BspACI CCGC 5 cut(s) 11, 82, 681, 963, 990
BspANI GGCC 5 cut(s) 58, 81, 477, 515, 975
BspCNI CTCAG 2 cut(s) 263, 817
BspDI ATCGAT 1 cut(s) 91
BspMI ACCTGC 2 cut(s) 186, 251
BspPI GGATC 9 cut(s) 60, 530, 543, 596, 650, 695, 750, 763, 1390
BspT107I GGYRCC 1 cut(s) 1075
BsrDI GCAATG 2 cut(s) 864, 1422
BsrGI TGTACA 1 cut(s) 1474
BsrI ACTGG 3 cut(s) 502, 1005, 1325
BssECI CCNNGG 9 cut(s) 43, 140, 712, 713, 759, 970, 1356, 1526, 1590
BssMI GATC 8 cut(s) 52, 343, 535, 601, 655, 700, 755, 1382
BssNI GRCGYC 1 cut(s) 1490
BssT1I CCWWGG 4 cut(s) 140, 759, 1356, 1590
Bst2UI CCWGG 2 cut(s) 714, 1034
Bst4CI ACNGT 3 cut(s) 131, 1165, 1345
Bst6I CTCTTC 4 cut(s) 711, 1057, 1479, 1604
BstACI GRCGYC 1 cut(s) 1490
BstAPI GCANNNNNTGC 1 cut(s) 208
BstAUI TGTACA 1 cut(s) 1474
BstC8I GCNNGC 1 cut(s) 1281
BstDEI CTNAG 4 cut(s) 250, 751, 769, 825
BstDSI CCRYGG 2 cut(s) 140, 1590
BstEII GGTNACC 1 cut(s) 244
BstENI CCTNNNNNAGG 1 cut(s) 750
BstF5I GGATG 8 cut(s) 181, 346, 373, 436, 820, 976, 1013, 1199
BstH2I RGCGCY 2 cut(s) 106, 1553
BstHHI GCGC 2 cut(s) 105, 1552
BstKTI GATC 8 cut(s) 55, 346, 538, 604, 658, 703, 758, 1385
BstMAI GTCTC 3 cut(s) 360, 821, 1522
BstMBI GATC 8 cut(s) 52, 343, 535, 601, 655, 700, 755, 1382
BstMWI GCNNNNNNNGC 8 cut(s) 208, 581, 680, 770, 917, 966, 1496, 1558
BstNI CCWGG 2 cut(s) 714, 1034
BstNSI RCATGY 1 cut(s) 1283
BstPI GGTNACC 1 cut(s) 244
BstSCI CCNGG 3 cut(s) 712, 969, 1032
BstSFI CTRYAG 1 cut(s) 483
BstSLI GKGCMC 1 cut(s) 479
BstV1I GCAGC 3 cut(s) 478, 850, 972
BstV2I GAAGAC 1 cut(s) 1110
BstX2I RGATCY 4 cut(s) 535, 700, 755, 1382
BstXI CCANNNNNNTGG 2 cut(s) 1040, 1597
BstYI RGATCY 4 cut(s) 535, 700, 755, 1382
Bsu15I ATCGAT 1 cut(s) 91
Bsu36I CCTNAGG 1 cut(s) 751
BsuRI GGCC 5 cut(s) 58, 81, 477, 515, 975
BsuTUI ATCGAT 1 cut(s) 91
BtgI CCRYGG 2 cut(s) 140, 1590
BtrI CACGTC 2 cut(s) 364, 406
BtsCI GGATG 8 cut(s) 181, 346, 373, 436, 820, 976, 1013, 1199
BtsI GCAGTG 2 cut(s) 906, 1203
BtsIMutI CAGTG 4 cut(s) 906, 998, 1203, 1318
BveI ACCTGC 2 cut(s) 186, 251
Cac8I GCNNGC 1 cut(s) 1281
CfoI GCGC 2 cut(s) 105, 1552
Cfr13I GGNCC 9 cut(s) 79, 263, 431, 475, 476, 514, 728, 973, 1683
ClaI ATCGAT 1 cut(s) 91
CseI GACGC 2 cut(s) 794, 1479
Csp6I GTAC 3 cut(s) 1155, 1367, 1475
CviAII CATG 6 cut(s) 141, 463, 581, 794, 1280, 1591
CviQI GTAC 3 cut(s) 1155, 1367, 1475
DdeI CTNAG 4 cut(s) 250, 751, 769, 825
DpnI GATC 8 cut(s) 54, 345, 537, 603, 657, 702, 757, 1384
DpnII GATC 8 cut(s) 52, 343, 535, 601, 655, 700, 755, 1382
Eam1104I CTCTTC 4 cut(s) 711, 1057, 1479, 1604
EarI CTCTTC 4 cut(s) 711, 1057, 1479, 1604
Eco130I CCWWGG 4 cut(s) 140, 759, 1356, 1590
Eco24I GRGCYC 2 cut(s) 169, 479
Eco47I GGWCC 4 cut(s) 263, 431, 728, 1683
Eco47III AGCGCT 1 cut(s) 1551
Eco57I CTGAAG 3 cut(s) 1104, 1407, 1421
Eco81I CCTNAGG 1 cut(s) 751
Eco88I CYCGRG 1 cut(s) 42
Eco91I GGTNACC 1 cut(s) 244
EcoNI CCTNNNNNAGG 1 cut(s) 750
EcoO109I RGGNCCY 3 cut(s) 263, 728, 1683
EcoO65I GGTNACC 1 cut(s) 244
EcoRI GAATTC 1 cut(s) 1324
EcoRII CCWGG 2 cut(s) 712, 1032
EcoT14I CCWWGG 4 cut(s) 140, 759, 1356, 1590
EcoT38I GRGCYC 2 cut(s) 169, 479
ErhI CCWWGG 4 cut(s) 140, 759, 1356, 1590
FaeI CATG 6 cut(s) 144, 466, 584, 797, 1283, 1594
FalI AAGNNNNNCTT 2 cut(s) 513, 545
FaqI GGGAC 2 cut(s) 674, 741
FatI CATG 6 cut(s) 140, 462, 580, 793, 1279, 1590
FauI CCCGC 1 cut(s) 688
FauNDI CATATG 1 cut(s) 471
Fnu4HI GCNGC 6 cut(s) 6, 82, 467, 839, 961, 990
FokI GGATG 8 cut(s) 168, 333, 380, 423, 827, 963, 1000, 1186
FriOI GRGCYC 2 cut(s) 169, 479
Fsp4HI GCNGC 6 cut(s) 6, 82, 467, 839, 961, 990
FspBI CTAG 3 cut(s) 170, 398, 834
GlaI GCGC 2 cut(s) 104, 1551
GluI GCNGC 6 cut(s) 6, 82, 467, 839, 961, 990
GsaI CCCAGC 1 cut(s) 52
GsuI CTGGAG 2 cut(s) 485, 830
HaeII RGCGCY 2 cut(s) 106, 1553
HaeIII GGCC 5 cut(s) 58, 81, 477, 515, 975
HapII CCGG 2 cut(s) 970, 1073
HgaI GACGC 2 cut(s) 794, 1479
HhaI GCGC 2 cut(s) 105, 1552
Hin1I GRCGYC 1 cut(s) 1490
Hin1II CATG 6 cut(s) 144, 466, 584, 797, 1283, 1594
Hin6I GCGC 2 cut(s) 103, 1550
HinP1I GCGC 2 cut(s) 103, 1550
HincII GTYRAC 1 cut(s) 1225
HindII GTYRAC 1 cut(s) 1225
HinfI GANTC 8 cut(s) 64, 88, 412, 446, 863, 1392, 1469, 1564
HpaII CCGG 2 cut(s) 970, 1073
HphI GGTGA 5 cut(s) 238, 421, 629, 701, 1372
Hpy166II GTNNAC 1 cut(s) 1225
Hpy188I TCNGA 6 cut(s) 63, 591, 606, 1387, 1414, 1468
Hpy188III TCNNGA 9 cut(s) 116, 135, 359, 440, 521, 809, 1263, 1312, 1380
Hpy8I GTNNAC 1 cut(s) 1225
HpyCH4III ACNGT 3 cut(s) 131, 1165, 1345
HpyCH4IV ACGT 2 cut(s) 363, 405
HpyCH4V TGCA 6 cut(s) 466, 1169, 1271, 1293, 1404, 1499
HpyF10VI GCNNNNNNNGC 8 cut(s) 208, 581, 680, 770, 917, 966, 1496, 1558
HpyF3I CTNAG 4 cut(s) 250, 751, 769, 825
HpySE526I ACGT 2 cut(s) 363, 405
Hsp92I GRCGYC 1 cut(s) 1490
Hsp92II CATG 6 cut(s) 144, 466, 584, 797, 1283, 1594
HspAI GCGC 2 cut(s) 103, 1550
Kzo9I GATC 8 cut(s) 52, 343, 535, 601, 655, 700, 755, 1382
LmnI GCTCC 4 cut(s) 13, 164, 321, 493
Lsp1109I GCAGC 3 cut(s) 478, 850, 972
LweI GCATC 5 cut(s) 582, 805, 1001, 1056, 1342
MaeI CTAG 3 cut(s) 170, 398, 834
MaeII ACGT 2 cut(s) 363, 405
MaeIII GTNAC 3 cut(s) 157, 244, 1491
MalI GATC 8 cut(s) 54, 345, 537, 603, 657, 702, 757, 1384
MboI GATC 8 cut(s) 52, 343, 535, 601, 655, 700, 755, 1382
MboII GAAGA 9 cut(s) 320, 536, 698, 782, 1044, 1110, 1249, 1496, 1621
MfeI CAATTG 1 cut(s) 1658
MflI RGATCY 4 cut(s) 535, 700, 755, 1382
MhlI GDGCHC 2 cut(s) 169, 479
MluCI AATT 9 cut(s) 124, 213, 278, 628, 779, 1324, 1446, 1628, 1658
MmeI TCCRAC 2 cut(s) 629, 808
MroXI GAANNNNTTC 1 cut(s) 1623
MseI TTAA 4 cut(s) 216, 288, 620, 1542
MslI CAYNNNNRTG 3 cut(s) 441, 792, 1595
MspA1I CMGCKG 4 cut(s) 84, 841, 963, 1441
MspI CCGG 2 cut(s) 970, 1073
MspR9I CCNGG 3 cut(s) 714, 971, 1034
MunI CAATTG 1 cut(s) 1658
MvaI CCWGG 2 cut(s) 714, 1034
MwoI GCNNNNNNNGC 8 cut(s) 208, 581, 680, 770, 917, 966, 1496, 1558
NciI CCSGG 1 cut(s) 971
NcoI CCATGG 2 cut(s) 140, 1590
NdeI CATATG 1 cut(s) 471
NdeII GATC 8 cut(s) 52, 343, 535, 601, 655, 700, 755, 1382
NlaIII CATG 6 cut(s) 144, 466, 584, 797, 1283, 1594
NmeAIII GCCGAG 1 cut(s) 1551
NmuCI GTSAC 3 cut(s) 157, 244, 1491
NspI RCATGY 1 cut(s) 1283
PaeI GCATGC 1 cut(s) 1283
PaqCI CACCTGC 1 cut(s) 186
PasI CCCWGGG 1 cut(s) 713
PdmI GAANNNNTTC 1 cut(s) 1623
PfeI GAWTC 8 cut(s) 64, 88, 412, 446, 863, 1392, 1469, 1564
PkrI GCNGC 6 cut(s) 7, 83, 468, 840, 962, 991
PpuMI RGGWCCY 3 cut(s) 263, 728, 1683
PshBI ATTAAT 1 cut(s) 216
Psp5II RGGWCCY 3 cut(s) 263, 728, 1683
Psp6I CCWGG 2 cut(s) 712, 1032
PspEI GGTNACC 1 cut(s) 244
PspFI CCCAGC 1 cut(s) 48
PspGI CCWGG 2 cut(s) 712, 1032
PspOMI GGGCCC 1 cut(s) 475
PspPI GGNCC 9 cut(s) 79, 263, 431, 475, 476, 514, 728, 973, 1683
PspPPI RGGWCCY 3 cut(s) 263, 728, 1683
PsuI RGATCY 4 cut(s) 535, 700, 755, 1382
PvuII CAGCTG 2 cut(s) 841, 1441
RsaI GTAC 3 cut(s) 1156, 1368, 1476
RsaNI GTAC 3 cut(s) 1155, 1367, 1475
RseI CAYNNNNRTG 3 cut(s) 441, 792, 1595
SaqAI TTAA 4 cut(s) 216, 288, 620, 1542
SatI GCNGC 6 cut(s) 6, 82, 467, 839, 961, 990
Sau3AI GATC 8 cut(s) 52, 343, 535, 601, 655, 700, 755, 1382
Sau96I GGNCC 9 cut(s) 79, 263, 431, 475, 476, 514, 728, 973, 1683
ScrFI CCNGG 3 cut(s) 714, 971, 1034
SduI GDGCHC 2 cut(s) 169, 479
SfaNI GCATC 5 cut(s) 582, 805, 1001, 1056, 1342
SfcI CTRYAG 1 cut(s) 483
SinI GGWCC 4 cut(s) 263, 431, 728, 1683
SmiMI CAYNNNNRTG 3 cut(s) 441, 792, 1595
SphI GCATGC 1 cut(s) 1283
Sse9I AATT 9 cut(s) 124, 213, 278, 628, 779, 1324, 1446, 1628, 1658
SsiI CCGC 5 cut(s) 11, 82, 681, 963, 990
SspMI CTAG 3 cut(s) 170, 398, 834
StyD4I CCNGG 3 cut(s) 712, 969, 1032
StyI CCWWGG 4 cut(s) 140, 759, 1356, 1590
TaaI ACNGT 3 cut(s) 131, 1165, 1345
TaiI ACGT 2 cut(s) 366, 408
TaqI TCGA 2 cut(s) 91, 1313
TaqII GACCGA 1 cut(s) 419
TasI AATT 9 cut(s) 124, 213, 278, 628, 779, 1324, 1446, 1628, 1658
TatI WGTACW 1 cut(s) 1474
TauI GCSGC 2 cut(s) 84, 992
TfiI GAWTC 8 cut(s) 64, 88, 412, 446, 863, 1392, 1469, 1564
Tru1I TTAA 4 cut(s) 216, 288, 620, 1542
Tru9I TTAA 4 cut(s) 216, 288, 620, 1542
TscAI CASTG 4 cut(s) 913, 1005, 1210, 1325
TseFI GTSAC 3 cut(s) 157, 244, 1491
TseI GCWGC 4 cut(s) 5, 466, 838, 960
Tsp45I GTSAC 3 cut(s) 157, 244, 1491
TspDTI ATGAA 6 cut(s) 271, 389, 782, 1044, 1188, 1411
TspGWI ACGGA 1 cut(s) 1667
TspRI CASTG 4 cut(s) 913, 1005, 1210, 1325
VpaK11BI GGWCC 4 cut(s) 263, 431, 728, 1683
VspI ATTAAT 1 cut(s) 216
XagI CCTNNNNNAGG 1 cut(s) 750
XapI RAATTY 2 cut(s) 278, 1324
XceI RCATGY 1 cut(s) 1283
XcmI CCANNNNNNNNNTGG 3 cut(s) 922, 1040, 1094
XmnI GAANNNNTTC 1 cut(s) 1623
XspI CTAG 3 cut(s) 170, 398, 834
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.