Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 568 |
| AatII | GACGTC | 1 cut(s) | 279 |
| AccI | GTMKAC | 1 cut(s) | 273 |
| AccII | CGCG | 3 cut(s) | 556, 569, 656 |
| AclWI | GGATC | 1 cut(s) | 252 |
| AcsI | RAATTY | 1 cut(s) | 195 |
| AcyI | GRCGYC | 3 cut(s) | 276, 371, 513 |
| AfaI | GTAC | 1 cut(s) | 587 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 223, 338, 538, 638, 786 |
| AflIII | ACRYGT | 2 cut(s) | 567, 913 |
| AgeI | ACCGGT | 2 cut(s) | 223, 292 |
| AgsI | TTSAA | 6 cut(s) | 188, 200, 233, 254, 446, 629 |
| AjnI | CCWGG | 1 cut(s) | 636 |
| AluBI | AGCT | 5 cut(s) | 18, 44, 89, 402, 696 |
| AluI | AGCT | 5 cut(s) | 18, 44, 89, 402, 696 |
| Alw21I | GWGCWC | 2 cut(s) | 91, 698 |
| Alw26I | GTCTC | 5 cut(s) | 17, 107, 137, 420, 552 |
| AlwI | GGATC | 1 cut(s) | 252 |
| Ama87I | CYCGRG | 3 cut(s) | 507, 685, 697 |
| AoxI | GGCC | 5 cut(s) | 158, 524, 529, 634, 884 |
| ApeKI | GCWGC | 1 cut(s) | 384 |
| ApoI | RAATTY | 1 cut(s) | 195 |
| AsiGI | ACCGGT | 2 cut(s) | 223, 292 |
| AspS9I | GGNCC | 8 cut(s) | 72, 213, 529, 590, 634, 708, 815, 885 |
| AsuHPI | GGTGA | 2 cut(s) | 499, 701 |
| AsuII | TTCGAA | 1 cut(s) | 830 |
| AvaI | CYCGRG | 3 cut(s) | 507, 685, 697 |
| AvaII | GGWCC | 5 cut(s) | 72, 213, 590, 708, 815 |
| BanII | GRGCYC | 5 cut(s) | 91, 698, 776, 809, 864 |
| BbsI | GAAGAC | 1 cut(s) | 438 |
| Bbv12I | GWGCWC | 2 cut(s) | 91, 698 |
| BbvCI | CCTCAGC | 1 cut(s) | 40 |
| BbvI | GCAGC | 1 cut(s) | 396 |
| BciT130I | CCWGG | 1 cut(s) | 638 |
| BciVI | GTATCC | 1 cut(s) | 804 |
| BcoDI | GTCTC | 5 cut(s) | 17, 107, 137, 420, 552 |
| BfmI | CTRYAG | 1 cut(s) | 389 |
| BfuI | GTATCC | 1 cut(s) | 804 |
| BglII | AGATCT | 1 cut(s) | 357 |
| BisI | GCNGC | 4 cut(s) | 95, 343, 346, 385 |
| BlsI | GCNGC | 4 cut(s) | 96, 344, 347, 386 |
| Bme18I | GGWCC | 5 cut(s) | 72, 213, 590, 708, 815 |
| BmeT110I | CYCGRG | 3 cut(s) | 507, 685, 697 |
| BmgT120I | GGNCC | 8 cut(s) | 72, 213, 529, 590, 634, 708, 815, 885 |
| BmiI | GGNNCC | 4 cut(s) | 215, 773, 806, 861 |
| BmrI | ACTGGG | 1 cut(s) | 325 |
| BmuI | ACTGGG | 1 cut(s) | 325 |
| BpiI | GAAGAC | 1 cut(s) | 438 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 73, 105, 389, 421 |
| Bpu10I | CCTNAGC | 1 cut(s) | 40 |
| Bpu14I | TTCGAA | 1 cut(s) | 830 |
| BsaHI | GRCGYC | 3 cut(s) | 276, 371, 513 |
| BsaI | GGTCTC | 1 cut(s) | 107 |
| BsaJI | CCNNGG | 6 cut(s) | 149, 506, 507, 508, 637, 685 |
| BsaWI | WCCGGW | 3 cut(s) | 223, 292, 817 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 356, 386, 574, 604 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 223, 338, 538, 638, 786 |
| Bse118I | RCCGGY | 5 cut(s) | 97, 223, 292, 484, 675 |
| Bse1I | ACTGG | 3 cut(s) | 179, 246, 320 |
| BseBI | CCWGG | 1 cut(s) | 638 |
| BseDI | CCNNGG | 6 cut(s) | 149, 506, 507, 508, 637, 685 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 223, 338, 538, 638, 786 |
| BseMII | CTCAG | 2 cut(s) | 28, 54 |
| BseNI | ACTGG | 3 cut(s) | 179, 246, 320 |
| BseRI | GAGGAG | 4 cut(s) | 10, 323, 872, 887 |
| BseXI | GCAGC | 1 cut(s) | 396 |
| Bsh1236I | CGCG | 3 cut(s) | 556, 569, 656 |
| Bsh1285I | CGRYCG | 1 cut(s) | 102 |
| BshFI | GGCC | 5 cut(s) | 160, 526, 531, 636, 886 |
| BshTI | ACCGGT | 2 cut(s) | 223, 292 |
| BsiEI | CGRYCG | 1 cut(s) | 102 |
| BsiHKAI | GWGCWC | 2 cut(s) | 91, 698 |
| BsiHKCI | CYCGRG | 3 cut(s) | 507, 685, 697 |
| BslFI | GGGAC | 1 cut(s) | 883 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 223, 338, 538, 638, 786 |
| BsmAI | GTCTC | 5 cut(s) | 17, 107, 137, 420, 552 |
| BsmBI | CGTCTC | 1 cut(s) | 420 |
| BsmFI | GGGAC | 1 cut(s) | 883 |
| BsmI | GAATGC | 2 cut(s) | 607, 932 |
| BsnI | GGCC | 5 cut(s) | 160, 526, 531, 636, 886 |
| Bso31I | GGTCTC | 1 cut(s) | 107 |
| BsoBI | CYCGRG | 3 cut(s) | 507, 685, 697 |
| Bsp119I | TTCGAA | 1 cut(s) | 830 |
| Bsp1286I | GDGCHC | 5 cut(s) | 91, 698, 776, 809, 864 |
| Bsp143I | GATC | 4 cut(s) | 190, 244, 357, 713 |
| BspANI | GGCC | 5 cut(s) | 160, 526, 531, 636, 886 |
| BspCNI | CTCAG | 2 cut(s) | 27, 53 |
| BspFNI | CGCG | 3 cut(s) | 556, 569, 656 |
| BspLI | GGNNCC | 4 cut(s) | 215, 773, 806, 861 |
| BspMAI | CTGCAG | 1 cut(s) | 393 |
| BspPI | GGATC | 1 cut(s) | 252 |
| BspT104I | TTCGAA | 1 cut(s) | 830 |
| BspTNI | GGTCTC | 1 cut(s) | 107 |
| BsrFI | RCCGGY | 5 cut(s) | 97, 223, 292, 484, 675 |
| BsrI | ACTGG | 3 cut(s) | 179, 246, 320 |
| BssAI | RCCGGY | 5 cut(s) | 97, 223, 292, 484, 675 |
| BssECI | CCNNGG | 6 cut(s) | 149, 506, 507, 508, 637, 685 |
| BssMI | GATC | 4 cut(s) | 190, 244, 357, 713 |
| BssNI | GRCGYC | 3 cut(s) | 276, 371, 513 |
| Bst2UI | CCWGG | 1 cut(s) | 638 |
| Bst4CI | ACNGT | 4 cut(s) | 118, 418, 590, 743 |
| Bst6I | CTCTTC | 4 cut(s) | 105, 203, 257, 317 |
| BstACI | GRCGYC | 3 cut(s) | 276, 371, 513 |
| BstBI | TTCGAA | 1 cut(s) | 830 |
| BstC8I | GCNNGC | 4 cut(s) | 369, 389, 720, 888 |
| BstDEI | CTNAG | 2 cut(s) | 14, 40 |
| BstEII | GGTNACC | 1 cut(s) | 418 |
| BstFNI | CGCG | 3 cut(s) | 556, 569, 656 |
| BstKTI | GATC | 4 cut(s) | 193, 247, 360, 716 |
| BstMAI | GTCTC | 5 cut(s) | 17, 107, 137, 420, 552 |
| BstMBI | GATC | 4 cut(s) | 190, 244, 357, 713 |
| BstMCI | CGRYCG | 1 cut(s) | 102 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 15, 393 |
| BstNI | CCWGG | 1 cut(s) | 638 |
| BstNSI | RCATGY | 1 cut(s) | 917 |
| BstPI | GGTNACC | 1 cut(s) | 418 |
| BstSFI | CTRYAG | 1 cut(s) | 389 |
| BstUI | CGCG | 3 cut(s) | 556, 569, 656 |
| BstV1I | GCAGC | 1 cut(s) | 396 |
| BstV2I | GAAGAC | 1 cut(s) | 438 |
| BstX2I | RGATCY | 1 cut(s) | 357 |
| BstYI | RGATCY | 1 cut(s) | 357 |
| BsuI | GTATCC | 1 cut(s) | 804 |
| BsuRI | GGCC | 5 cut(s) | 160, 526, 531, 636, 886 |
| BtsIMutI | CAGTG | 1 cut(s) | 186 |
| Cac8I | GCNNGC | 4 cut(s) | 369, 389, 720, 888 |
| Cfr10I | RCCGGY | 5 cut(s) | 97, 223, 292, 484, 675 |
| Cfr13I | GGNCC | 8 cut(s) | 72, 213, 529, 590, 634, 708, 815, 885 |
| Cfr9I | CCCGGG | 2 cut(s) | 507, 685 |
| CpoI | CGGWCCG | 1 cut(s) | 590 |
| CseI | GACGC | 6 cut(s) | 360, 502, 558, 562, 575, 645 |
| Csp6I | GTAC | 1 cut(s) | 586 |
| CspAI | ACCGGT | 2 cut(s) | 223, 292 |
| CspI | CGGWCCG | 1 cut(s) | 590 |
| CviAII | CATG | 1 cut(s) | 914 |
| CviQI | GTAC | 1 cut(s) | 586 |
| DdeI | CTNAG | 2 cut(s) | 14, 40 |
| DpnI | GATC | 4 cut(s) | 192, 246, 359, 715 |
| DpnII | GATC | 4 cut(s) | 190, 244, 357, 713 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 568 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 568 |
| Eam1104I | CTCTTC | 4 cut(s) | 105, 203, 257, 317 |
| EarI | CTCTTC | 4 cut(s) | 105, 203, 257, 317 |
| EciI | GGCGGA | 3 cut(s) | 13, 81, 320 |
| Ecl136II | GAGCTC | 2 cut(s) | 89, 696 |
| Eco24I | GRGCYC | 5 cut(s) | 91, 698, 776, 809, 864 |
| Eco31I | GGTCTC | 1 cut(s) | 107 |
| Eco47I | GGWCC | 5 cut(s) | 72, 213, 590, 708, 815 |
| Eco53kI | GAGCTC | 2 cut(s) | 89, 696 |
| Eco88I | CYCGRG | 3 cut(s) | 507, 685, 697 |
| Eco91I | GGTNACC | 1 cut(s) | 418 |
| EcoICRI | GAGCTC | 2 cut(s) | 89, 696 |
| EcoO109I | RGGNCCY | 1 cut(s) | 213 |
| EcoO65I | GGTNACC | 1 cut(s) | 418 |
| EcoRII | CCWGG | 1 cut(s) | 636 |
| EcoT38I | GRGCYC | 5 cut(s) | 91, 698, 776, 809, 864 |
| Esp3I | CGTCTC | 1 cut(s) | 420 |
| FaeI | CATG | 1 cut(s) | 917 |
| FaiI | YATR | 2 cut(s) | 915, 943 |
| FaqI | GGGAC | 1 cut(s) | 883 |
| FatI | CATG | 1 cut(s) | 913 |
| FauI | CCCGC | 2 cut(s) | 642, 895 |
| FblI | GTMKAC | 1 cut(s) | 273 |
| Fnu4HI | GCNGC | 4 cut(s) | 95, 343, 346, 385 |
| FriOI | GRGCYC | 5 cut(s) | 91, 698, 776, 809, 864 |
| Fsp4HI | GCNGC | 4 cut(s) | 95, 343, 346, 385 |
| GluI | GCNGC | 4 cut(s) | 95, 343, 346, 385 |
| HaeIII | GGCC | 5 cut(s) | 160, 526, 531, 636, 886 |
| HgaI | GACGC | 6 cut(s) | 360, 502, 558, 562, 575, 645 |
| Hin1I | GRCGYC | 3 cut(s) | 276, 371, 513 |
| Hin1II | CATG | 1 cut(s) | 917 |
| HincII | GTYRAC | 2 cut(s) | 274, 460 |
| HindII | GTYRAC | 2 cut(s) | 274, 460 |
| HinfI | GANTC | 2 cut(s) | 668, 896 |
| HphI | GGTGA | 2 cut(s) | 499, 701 |
| Hpy166II | GTNNAC | 3 cut(s) | 106, 274, 460 |
| Hpy188III | TCNNGA | 5 cut(s) | 67, 188, 200, 254, 711 |
| Hpy8I | GTNNAC | 3 cut(s) | 106, 274, 460 |
| HpyAV | CCTTC | 1 cut(s) | 115 |
| HpyCH4III | ACNGT | 4 cut(s) | 118, 418, 590, 743 |
| HpyCH4IV | ACGT | 1 cut(s) | 276 |
| HpyCH4V | TGCA | 3 cut(s) | 384, 391, 605 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 15, 393 |
| HpyF3I | CTNAG | 2 cut(s) | 14, 40 |
| HpySE526I | ACGT | 1 cut(s) | 276 |
| Hsp92I | GRCGYC | 3 cut(s) | 276, 371, 513 |
| Hsp92II | CATG | 1 cut(s) | 917 |
| Kzo9I | GATC | 4 cut(s) | 190, 244, 357, 713 |
| LmnI | GCTCC | 7 cut(s) | 23, 49, 94, 364, 771, 804, 859 |
| Lsp1109I | GCAGC | 1 cut(s) | 396 |
| MaeII | ACGT | 1 cut(s) | 276 |
| MaeIII | GTNAC | 2 cut(s) | 418, 680 |
| MalI | GATC | 4 cut(s) | 192, 246, 359, 715 |
| MboI | GATC | 4 cut(s) | 190, 244, 357, 713 |
| MboII | GAAGA | 5 cut(s) | 122, 220, 274, 334, 443 |
| MflI | RGATCY | 1 cut(s) | 357 |
| MhlI | GDGCHC | 5 cut(s) | 91, 698, 776, 809, 864 |
| MluCI | AATT | 2 cut(s) | 195, 491 |
| MluI | ACGCGT | 1 cut(s) | 567 |
| MlyI | GAGTC | 2 cut(s) | 677, 905 |
| MmeI | TCCRAC | 7 cut(s) | 99, 354, 560, 575, 683, 749, 782 |
| MseI | TTAA | 1 cut(s) | 750 |
| Mva1269I | GAATGC | 2 cut(s) | 607, 932 |
| MvaI | CCWGG | 1 cut(s) | 638 |
| MvnI | CGCG | 3 cut(s) | 556, 569, 656 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 15, 393 |
| NdeII | GATC | 4 cut(s) | 190, 244, 357, 713 |
| NlaIII | CATG | 1 cut(s) | 917 |
| NlaIV | GGNNCC | 4 cut(s) | 215, 773, 806, 861 |
| NmeAIII | GCCGAG | 1 cut(s) | 386 |
| NmuCI | GTSAC | 1 cut(s) | 680 |
| NspI | RCATGY | 1 cut(s) | 917 |
| NspV | TTCGAA | 1 cut(s) | 830 |
| PciI | ACATGT | 1 cut(s) | 913 |
| PcsI | WCGNNNNNNNCGW | 3 cut(s) | 114, 368, 836 |
| PctI | GAATGC | 2 cut(s) | 607, 932 |
| PflFI | GACNNNGTC | 1 cut(s) | 667 |
| PinAI | ACCGGT | 2 cut(s) | 223, 292 |
| PkrI | GCNGC | 4 cut(s) | 96, 344, 347, 386 |
| PleI | GAGTC | 2 cut(s) | 676, 904 |
| PpsI | GAGTC | 2 cut(s) | 676, 904 |
| PpuMI | RGGWCCY | 1 cut(s) | 213 |
| PscI | ACATGT | 1 cut(s) | 913 |
| Psp124BI | GAGCTC | 2 cut(s) | 91, 698 |
| Psp5II | RGGWCCY | 1 cut(s) | 213 |
| Psp6I | CCWGG | 1 cut(s) | 636 |
| PspEI | GGTNACC | 1 cut(s) | 418 |
| PspGI | CCWGG | 1 cut(s) | 636 |
| PspN4I | GGNNCC | 4 cut(s) | 215, 773, 806, 861 |
| PspPI | GGNCC | 8 cut(s) | 72, 213, 529, 590, 634, 708, 815, 885 |
| PspPPI | RGGWCCY | 1 cut(s) | 213 |
| PstI | CTGCAG | 1 cut(s) | 393 |
| PsuI | RGATCY | 1 cut(s) | 357 |
| PsyI | GACNNNGTC | 1 cut(s) | 667 |
| RsaI | GTAC | 1 cut(s) | 587 |
| RsaNI | GTAC | 1 cut(s) | 586 |
| Rsr2I | CGGWCCG | 1 cut(s) | 590 |
| RsrII | CGGWCCG | 1 cut(s) | 590 |
| SacI | GAGCTC | 2 cut(s) | 91, 698 |
| SalI | GTCGAC | 1 cut(s) | 272 |
| SaqAI | TTAA | 1 cut(s) | 750 |
| SatI | GCNGC | 4 cut(s) | 95, 343, 346, 385 |
| Sau3AI | GATC | 4 cut(s) | 190, 244, 357, 713 |
| Sau96I | GGNCC | 8 cut(s) | 72, 213, 529, 590, 634, 708, 815, 885 |
| SchI | GAGTC | 2 cut(s) | 677, 905 |
| SduI | GDGCHC | 5 cut(s) | 91, 698, 776, 809, 864 |
| SfcI | CTRYAG | 1 cut(s) | 389 |
| SfuI | TTCGAA | 1 cut(s) | 830 |
| SgrAI | CRCCGGYG | 1 cut(s) | 484 |
| SgrDI | CGTCGACG | 1 cut(s) | 272 |
| SinI | GGWCC | 5 cut(s) | 72, 213, 590, 708, 815 |
| SmaI | CCCGGG | 2 cut(s) | 509, 687 |
| Sse9I | AATT | 2 cut(s) | 195, 491 |
| SspI | AATATT | 1 cut(s) | 852 |
| SstI | GAGCTC | 2 cut(s) | 91, 698 |
| TaaI | ACNGT | 4 cut(s) | 118, 418, 590, 743 |
| TaiI | ACGT | 1 cut(s) | 279 |
| TaqI | TCGA | 5 cut(s) | 273, 659, 671, 830, 839 |
| TasI | AATT | 2 cut(s) | 195, 491 |
| TauI | GCSGC | 3 cut(s) | 97, 345, 348 |
| Tru1I | TTAA | 1 cut(s) | 750 |
| Tru9I | TTAA | 1 cut(s) | 750 |
| TscAI | CASTG | 1 cut(s) | 186 |
| TseFI | GTSAC | 1 cut(s) | 680 |
| TseI | GCWGC | 1 cut(s) | 384 |
| Tsp45I | GTSAC | 1 cut(s) | 680 |
| TspDTI | ATGAA | 1 cut(s) | 922 |
| TspGWI | ACGGA | 3 cut(s) | 366, 578, 680 |
| TspMI | CCCGGG | 2 cut(s) | 507, 685 |
| TspRI | CASTG | 1 cut(s) | 186 |
| Tth111I | GACNNNGTC | 1 cut(s) | 667 |
| VpaK11BI | GGWCC | 5 cut(s) | 72, 213, 590, 708, 815 |
| XapI | RAATTY | 1 cut(s) | 195 |
| XceI | RCATGY | 1 cut(s) | 917 |
| XmaI | CCCGGG | 2 cut(s) | 507, 685 |
| XmiI | GTMKAC | 1 cut(s) | 273 |
| ZraI | GACGTC | 1 cut(s) | 277 |