Rh5BG112600

Domain of unknown function (DUF4283)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000073
Physical Location & Seq
Forward (+)
10554333 .. 10560463
6131 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG112600.1

Sequence Viewer

Length: 2034 bp
ATGTCCTTTCATGACCACCTTGCCTCCCGGCTAGAGAACACATGTTGTCTGGAGGAAGTCGAGAACGAGATTTCCCTCATTGGTGCAATCATAGCTGATGTTGTCCCTAACACTGGTGGAATCAAAGGCTCTCTCAGATCCTTCTGGAAGGACATTGGCAGCTTTAAGCTCGCCCACCTCCATCACAACTTGTTCTCTATCAAGGCAACCCCAAAAGGTGCCTCCAAACTTCTGGAGGGTGGTCCCTGGCATGTGGATAACATGAGGTTCAACGTGGTAAAGTGGCTCCCCAACCTCACTATCAATGATATTCACTTCCATAAGGTTTGGTATTGGGTTCAGATTTCAGGACTCACGAGAGAAAAGATGAACACCCAGAATGCCAGGTTGATTGGGTCTGAGATCGGGTCAGTTCTGGAGATAGAAGACCCCGACAAGGTTGATGCAACTCTTCATGGTTTCCTGAGAGTGAAGGTTCTCATGGATTCCAGAAACCCCTTCCCTACTGGTTTCTGGCTTCCTCTAAACAAATACACCTCAACCAGGGTCGACTATGCTTACGAAGGCCTCTGTGACTTCTGTTACAAATGTGGGCGGCTAGGGCACCACCTGGATGTCTGCAAGAATTTTAGACTTATCAACCTTGAGGAGGGTGAGCCGGAAAATATTTGGTACGGCCCATGGATGAAGAGGAAGCCCCTCTCTATCAAACAGCAATCTTGGCTTGAGCGGCCGACGAGGGTTTACCGGAAAAAGCCTAAAACAAGGAGGTCCAACGAGCTAGGGTTTGATTCCCCGTATGCATCACATGCAAGCACTAGCTACCAGACCCAAGGCCACAGCTCTGAAACAACGTTGGCTGACAAGGATGTGACATGCCTTCTGATCTCTGAGCCTGATGATGAAGACCTGTCATCCCACAATTTAAATGTTCAAATCACTCCCGTGAGAAACCCCGGCACTTGTCACCTTTTGTCCCTTGGTCCACATGCTTTGCCTGTTACAGCCCCAAAGATCAGAGTTCAAAAAATCTCATATCGGAATACATTGGCGCTCTCTTTCTCCTTTGTCAATTCAATCAGCCTCATTCGCTTAGGGTTGCTACACTTGGTTAAGAACACAGCGTCGAAGTACTTTGAGGTCAACGCAAATTTCGAAAGAGGAGGTGGAGGAGACCACAGTGGTGATTGGACGACCCAAATGATGATCCATTTGAGCTACGTCTACGAGCAAGGTTTCGTCACAAATCTACAATCTCGATGCGAATCTCTGCCTTCTTCATCTCTATCAGAACGTGACCATGGAGATGGATGCAACTGCTGCTGGTACGTTGAGGAGGAGGACCTCATGAGATTGGGTGGCACTGATGGCAATTCCCACTCTCGATCCAGCCTTTGTCTCTTCCTAATTCCAGAAAGAGTGACCATCCTCAGAGCTAAAAAATCCTCTCCATTGTCCGCCATACTAAACAAGCTCGAGACTCCACAACTCCCAAAAGGTAAGAAACTCTTGACGTCGCTTTCAATTTTTGGCCATATTTTGCACTACTTTGATCATTGTTTTAATCTAGGAAACTATGTAATCTGCACCCCAACTTATAAGGATGAAGAAAAAATGAGAGCTTTACCCCTCTCTCTCTGGCGGCGGCAAACCCTAGCCTTAGGGTTTTGCTCGTCGCGAACCTTGGCCTCCGATGAGTCTCCTTCTCCTCGTGAAGCCTGTCTATCGCCCTCCTCGAGGTCGTGCTTCCCCTCTGTTCAACTTGTGGGTTGTGGAATCCGTTTTTTGGAGTGCTTCTCGGACTTTTGTGGTGCTAACAGGAAGTCTTTTCTGGAGTCCGATTGCTTGAGAGATATTTGCCGGAGGAGAGCATTCTCCAGAATGGATTTGTGGGCAGCAATTCGAATTTGGGATCCTGGATCTATTTATTCTTTTTCGCAGGGGATGAGATGTTGCTGCGATGGTGGCGGGCAGGCTTGGTGGTGGTTGCCGGTGACAGAGGAGGTTGGGATGCAATCCGATTTGGGAGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 zf-CCHC_4 zf-RING_2

Protein Analysis

677

Amino Acids

76.47

Weight (kDa)

8.06

Isoelectric Point (pI)

49.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4283 PF14111 21 - 100 6.8e-06 Domain of unknown function (DUF4283)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000073)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g17821 FvH4_2g04610 FvH4_2g09611 FvH4_2g13563 FvH4_3g21493 FvH4_3g32115 FvH4_3g35060 FvH4_4g00220 FvH4_4g06410 FvH4_4g10367 FvH4_4g10367 FvH4_4g10367 FvH4_5g06101 FvH4_5g08842 FvH4_5g27366 FvH4_5g31283 FvH4_6g11611 FvH4_6g23032 FvH4_7g05661 FvH4_7g06172
malus_domestica MD13G1263700.v1.1
prunus_persica Prupe.1G292300_v2.0.a1 Prupe.5G009700_v2.0.a1 Prupe.6G158100_v2.0.a1 Prupe.8G025100_v2.0.a1 Prupe.8G042600_v2.0.a1
pyrus_communis pycom02g02250 pycom02g16030 pycom05g10490 pycom09g06450 pycom09g06460 pycom13g26200 pycom16g17700
rosa_chinensis RchiOBHm_Chr2g0163321 RchiOBHm_Chr4g0401981 RchiOBHm_Chr5g0005991 RchiOBHm_Chr5g0027191 RchiOBHm_Chr6g0249771
rosa_laevigata RLG00000001040 RLG00000001764 RLG00000002239 RLG00000002240 RLG00000003259 RLG00000008993 RLG00000013847 RLG00000014178 RLG00000015576 RLG00000017269 RLG00000017355 RLG00000018127 RLG00000018602 RLG00000018615 RLG00000019904 RLG00000020044 RLG00000021849 RLG00000021850 RLG00000027965 RLG00000029266 RLG00000029820 RLG00000031112 RLG00000031915 RLG00000033568 RLG00000035013 RLG00000035677 RLG00000036568 RLG00000036628
rosa_multiflora Rmu_co8254085.1_g000001 Rmu_co8462383.1_g000001 Rmu_sc0000185.1_g000049 Rmu_sc0000251.1_g000018 Rmu_sc0000367.1_g000094 Rmu_sc0000498.1_g000023 Rmu_sc0000868.1_g000005 Rmu_sc0001521.1_g000047 Rmu_sc0001534.1_g000007 Rmu_sc0002014.1_g000012 Rmu_sc0003214.1_g000009 Rmu_sc0003573.1_g000041 Rmu_sc0003832.1_g000023 Rmu_sc0004677.1_g000015 Rmu_sc0004967.1_g000014 Rmu_sc0005426.1_g000001 Rmu_sc0005621.1_g000004 Rmu_sc0005640.1_g000009 Rmu_sc0005947.1_g000034 Rmu_sc0006889.1_g000011 Rmu_sc0007121.1_g000014 Rmu_sc0009191.1_g000017 Rmu_sc0010556.1_g000011 Rmu_sc0011396.1_g000007 Rmu_sc0019862.1_g000002 Rmu_sc0022931.1_g000003 Rmu_sc0024164.1_g000001 Rmu_ssc0000391.1_g000027
rosa_roxburghii Rroxscaffold_1G00050060 Rroxscaffold_1G00061810 Rroxscaffold_2G00109590 Rroxscaffold_2G00113810 Rroxscaffold_2G00124040 Rroxscaffold_2G00143620 Rroxscaffold_2G00154760 Rroxscaffold_4G00302820 Rroxscaffold_4G00302830 Rroxscaffold_4G00308680 Rroxscaffold_4G00310240 Rroxscaffold_4G00327890 Rroxscaffold_5G00334600 Rroxscaffold_5G00336050 Rroxscaffold_5G00346560 Rroxscaffold_5G00347860 Rroxscaffold_5G00347870 Rroxscaffold_5G00349330 Rroxscaffold_5G00351980 Rroxscaffold_6G00395280 Rroxscaffold_6G00396750 Rroxscaffold_6G00402910 Rroxscaffold_7G00174790 Rroxscaffold_7G00177570 Rroxscaffold_7G00191820 Rroxscaffold_7G00203480 Rroxscaffold_7G00204380
rosa_rugosa Rorug01G0106600 Rorug01G0106600 Rorug01G0148500.1 Rorug02G0224200 Rorug02G0225500 Rorug02G0233000 Rorug02G0630400 Rorug03G0198000 Rorug03G0232600.1 Rorug03G0240500 Rorug04G0032900 Rorug04G0073300 Rorug04G0118500 Rorug04G0149600.1 Rorug04G0205100 Rorug04G0256700 Rorug05G0020200 Rorug05G0050000 Rorug05G0247300 Rorug05G0295100 Rorug05G0337900 Rorug05G0542600 Rorug06G0034800 Rorug06G0035100 Rorug06G0220100 Rorug07G0091900 Rorug07G0271200
rosa_samantha Rh1AG118200 Rh1AG253000 Rh1AG296300 Rh1AG409000 Rh1AG410600 Rh1BG073400 Rh1BG130400 Rh1BG147300 Rh1BG163600 Rh1CG016000 Rh1DG012200 Rh1DG012300 Rh1DG078600 Rh1DG078800 Rh1DG138100 Rh1DG179200 Rh1DG192600 Rh1DG194500 Rh1DG194600 Rh1DG214300 Rh1DG245900 Rh1DG267000 Rh1DG322700 Rh1DG442700 Rh2AG282000 Rh2AG423000 Rh2AG423100 Rh2AG460700 Rh2AG466200 Rh2AG513400 Rh2AG547000 Rh2BG277800 Rh2BG281200 Rh2BG298300 Rh2BG304300 Rh2BG433500 Rh2CG276000 Rh2CG282600 Rh2CG325500 Rh2CG393000 Rh2DG319200 Rh2DG443400 Rh3AG169300 Rh3AG303600 Rh3BG195000 Rh3BG282600 Rh3BG343400 Rh3BG343500 Rh3BG344100 Rh3CG111700 Rh3CG185700 Rh3CG259100 Rh3CG293000 Rh3CG317900 Rh3CG340900 Rh3CG341000 Rh3CG341100 Rh3CG354700 Rh3CG359200 Rh3CG368100 Rh3DG179900 Rh3DG288700 Rh3DG302600 Rh3DG316000 Rh3DG324500 Rh3DG324600 Rh3DG343200 Rh3DG343300 Rh3DG362400 Rh4AG071800 Rh4BG034000 Rh4BG044300 Rh4BG044400 Rh4BG069600 Rh4BG157400 Rh4CG007000 Rh4CG030400 Rh4CG037900 Rh4CG077700 Rh4CG115000 Rh4CG162400 Rh4CG162500 Rh4CG165500 Rh4CG165600 Rh4CG172900 Rh4CG239700 Rh4DG055900 Rh4DG173200 Rh5AG354900 Rh5AG511100 Rh5BG112600 Rh5BG541000 Rh5BG541100 Rh5BG549300 Rh5CG004400 Rh5CG315000 Rh5CG563800 Rh5CG572100 Rh5DG112200 Rh5DG191300 Rh5DG407600 Rh5DG407700 Rh5DG567500 Rh6AG002600 Rh6AG051400 Rh6AG199800 Rh6AG199900 Rh6AG206200 Rh6AG391000 Rh6AG391100 Rh6BG007500 Rh6BG053400 Rh6BG225900 Rh6BG227100 Rh6BG232400 Rh6CG091100 Rh6CG125400 Rh6CG186700 Rh6CG222700 Rh6CG222800 Rh6CG235500 Rh6CG247200 Rh6CG331200 Rh6CG465100 Rh6DG183500 Rh6DG213500 Rh6DG238800 Rh6DG263700 Rh7AG211800 Rh7AG441800 Rh7BG008600 Rh7BG253000 Rh7BG390000 Rh7CG189600 Rh7CG401500 Rh7CG424200 Rh7CG428700 Rh7CG479800 Rh7CG494300 Rh7DG177000 Rh7DG218700 Rh7DG254000 Rh7DG294800 Rh7DG407200 Rh7DG448800 Rh7DG461500
rosa_wichuraiana Rw0G012180 Rw1G005270 Rw1G012310 Rw1G016890 Rw1G021700 Rw2G014380 Rw2G027690 Rw3G016400 Rw3G028230 Rw3G029320 Rw4G002060 Rw4G002100 Rw4G003980 Rw4G014140 Rw4G014770 Rw4G014780 Rw4G015760 Rw4G021230 Rw5G000520 Rw5G035610 Rw6G001680 Rw6G017890 Rw6G027440 Rw6G034130 Rw6G034170 Rw7G012430 Rw7G033870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1599
AatII GACGTC 1 cut(s) 1517
AbsI CCTCGAGG 1 cut(s) 1735
AccB1I GGYRCC 2 cut(s) 218, 603
AccBSI CCGCTC 1 cut(s) 730
AccI GTMKAC 2 cut(s) 549, 1224
AccII CGCG 1 cut(s) 1678
AciI CCGC 6 cut(s) 595, 730, 1458, 1642, 1645, 1968
AclI AACGTT 1 cut(s) 854
AclWI GGATC 6 cut(s) 132, 1201, 1380, 1907, 1920, 1927
AcoI YGGCCR 2 cut(s) 731, 1531
AcsI RAATTY 3 cut(s) 625, 1150, 1905
AcyI GRCGYC 1 cut(s) 1514
AfaI GTAC 3 cut(s) 674, 1133, 1328
AfiI CCNNNNNNNGG 6 cut(s) 113, 436, 543, 649, 1737, 1786
AflIII ACRYGT 1 cut(s) 41
AgsI TTSAA 6 cut(s) 271, 935, 1025, 1077, 1524, 1760
AjnI CCWGG 5 cut(s) 245, 383, 542, 609, 1915
AleI CACNNNNGTG 2 cut(s) 944, 1182
AloI GAACNNNNNNTCC 2 cut(s) 56, 88
Alw26I GTCTC 4 cut(s) 1167, 1403, 1472, 1704
AlwI GGATC 6 cut(s) 132, 1201, 1380, 1907, 1920, 1927
Ama87I CYCGRG 2 cut(s) 1475, 1735
AoxI GGCC 6 cut(s) 565, 676, 731, 835, 1531, 1686
ApeKI GCWGC 4 cut(s) 159, 1320, 1895, 1956
ApoI RAATTY 3 cut(s) 625, 1150, 1905
AspLEI GCGC 1 cut(s) 1054
AspS9I GGNCC 5 cut(s) 242, 677, 771, 983, 1342
AsuC2I CCSGG 2 cut(s) 28, 957
AsuHPI GGTGA 4 cut(s) 665, 959, 1196, 2005
AsuII TTCGAA 2 cut(s) 1155, 1903
AvaI CYCGRG 2 cut(s) 1475, 1735
AvaII GGWCC 4 cut(s) 242, 771, 983, 1342
AxyI CCTNAGG 1 cut(s) 1660
BaeGI GKGCMC 1 cut(s) 606
BalI TGGCCA 1 cut(s) 1533
BamHI GGATCC 1 cut(s) 1912
BanI GGYRCC 2 cut(s) 218, 603
BauI CACGAG 2 cut(s) 355, 1710
BbsI GAAGAC 2 cut(s) 432, 912
BbvI GCAGC 4 cut(s) 171, 1307, 1907, 1943
BccI CCATC 5 cut(s) 189, 1301, 1361, 1433, 1955
BceAI ACGGC 1 cut(s) 691
BciT130I CCWGG 5 cut(s) 247, 385, 544, 611, 1917
BclI TGATCA 1 cut(s) 1552
BcnI CCSGG 2 cut(s) 28, 957
BcoDI GTCTC 4 cut(s) 1167, 1403, 1472, 1704
BfaI CTAG 6 cut(s) 32, 599, 782, 819, 1568, 1655
BfoI RGCGCY 1 cut(s) 1055
BisI GCNGC 8 cut(s) 160, 596, 731, 1321, 1643, 1646, 1896, 1957
BlsI GCNGC 8 cut(s) 161, 597, 732, 1322, 1644, 1647, 1897, 1958
BmcAI AGTACT 1 cut(s) 1133
Bme1390I CCNGG 7 cut(s) 28, 247, 385, 544, 611, 957, 1917
Bme18I GGWCC 4 cut(s) 242, 771, 983, 1342
BmeT110I CYCGRG 2 cut(s) 1475, 1735
BmgT120I GGNCC 5 cut(s) 242, 677, 771, 983, 1342
BmiI GGNNCC 5 cut(s) 220, 244, 287, 605, 1914
BmrFI CCNGG 7 cut(s) 28, 247, 385, 544, 611, 957, 1917
BmsI GCATC 5 cut(s) 433, 812, 1250, 1301, 2001
BpiI GAAGAC 2 cut(s) 432, 912
BplI GAGNNNNNCTC 2 cut(s) 1781, 1813
BpmI CTGGAG 5 cut(s) 71, 254, 437, 1853, 1861
Bpu10I CCTNAGC 1 cut(s) 1093
Bpu14I TTCGAA 2 cut(s) 1155, 1903
BpuEI CTTGAG 3 cut(s) 665, 746, 1867
BpuMI CCSGG 2 cut(s) 28, 957
BsaBI GATNNNNATC 1 cut(s) 1264
BsaHI GRCGYC 1 cut(s) 1514
BsaI GGTCTC 1 cut(s) 1167
BsaJI CCNNGG 8 cut(s) 245, 543, 680, 832, 955, 979, 1300, 1683
BsaWI WCCGGW 1 cut(s) 747
BsaXI ACNNNNNCTCC 2 cut(s) 8, 38
Bsc4I CCNNNNNNNGG 6 cut(s) 113, 436, 543, 649, 1737, 1786
Bse118I RCCGGY 1 cut(s) 1990
Bse1I ACTGG 2 cut(s) 118, 511
Bse21I CCTNAGG 1 cut(s) 1660
Bse8I GATNNNNATC 1 cut(s) 1264
BseBI CCWGG 5 cut(s) 247, 385, 544, 611, 1917
BseDI CCNNGG 8 cut(s) 245, 543, 680, 832, 955, 979, 1300, 1683
BseGI GGATG 9 cut(s) 619, 690, 874, 914, 1316, 1425, 1609, 1950, 2016
BseJI GATNNNNATC 1 cut(s) 1264
BseLI CCNNNNNNNGG 6 cut(s) 113, 436, 543, 649, 1737, 1786
BseMII CTCAG 5 cut(s) 148, 390, 455, 882, 1444
BseNI ACTGG 2 cut(s) 118, 511
BseRI GAGGAG 9 cut(s) 662, 1176, 1185, 1349, 1352, 1698, 1723, 1879, 2015
BseSI GKGCMC 1 cut(s) 606
BseX3I CGGCCG 1 cut(s) 731
BseXI GCAGC 4 cut(s) 171, 1307, 1907, 1943
BsgI GTGCAG 1 cut(s) 1570
Bsh1236I CGCG 1 cut(s) 1678
Bsh1285I CGRYCG 1 cut(s) 734
BshFI GGCC 6 cut(s) 567, 678, 733, 837, 1533, 1688
BshNI GGYRCC 2 cut(s) 218, 603
BsiEI CGRYCG 1 cut(s) 734
BsiHKCI CYCGRG 2 cut(s) 1475, 1735
BsiSI CCGG 6 cut(s) 28, 659, 748, 957, 1861, 1991
BslFI GGGAC 3 cut(s) 89, 228, 961
BslI CCNNNNNNNGG 6 cut(s) 113, 436, 543, 649, 1737, 1786
BsmAI GTCTC 4 cut(s) 1167, 1403, 1472, 1704
BsmFI GGGAC 3 cut(s) 89, 228, 961
BsmI GAATGC 2 cut(s) 385, 1871
BsnI GGCC 6 cut(s) 567, 678, 733, 837, 1533, 1688
Bso31I GGTCTC 1 cut(s) 1167
BsoBI CYCGRG 2 cut(s) 1475, 1735
Bsp119I TTCGAA 2 cut(s) 1155, 1903
Bsp1286I GDGCHC 1 cut(s) 606
Bsp143I GATC 9 cut(s) 137, 402, 885, 1014, 1206, 1385, 1552, 1912, 1919
Bsp19I CCATGG 2 cut(s) 680, 1300
Bsp68I TCGCGA 1 cut(s) 1678
BspACI CCGC 6 cut(s) 595, 730, 1458, 1642, 1645, 1968
BspANI GGCC 6 cut(s) 567, 678, 733, 837, 1533, 1688
BspCNI CTCAG 5 cut(s) 147, 391, 456, 883, 1443
BspFNI CGCG 1 cut(s) 1678
BspHI TCATGA 2 cut(s) 10, 1347
BspLI GGNNCC 5 cut(s) 220, 244, 287, 605, 1914
BspPI GGATC 6 cut(s) 132, 1201, 1380, 1907, 1920, 1927
BspT104I TTCGAA 2 cut(s) 1155, 1903
BspT107I GGYRCC 2 cut(s) 218, 603
BspTNI GGTCTC 1 cut(s) 1167
BsrBI CCGCTC 1 cut(s) 730
BsrFI RCCGGY 1 cut(s) 1990
BsrI ACTGG 2 cut(s) 118, 511
BssAI RCCGGY 1 cut(s) 1990
BssECI CCNNGG 8 cut(s) 245, 543, 680, 832, 955, 979, 1300, 1683
BssMI GATC 9 cut(s) 137, 402, 885, 1014, 1206, 1385, 1552, 1912, 1919
BssNI GRCGYC 1 cut(s) 1514
BssSI CACGAG 2 cut(s) 355, 1710
BssT1I CCWWGG 5 cut(s) 680, 832, 979, 1300, 1683
Bst2BI CACGAG 2 cut(s) 355, 1710
Bst2UI CCWGG 5 cut(s) 247, 385, 544, 611, 1917
Bst4CI ACNGT 1 cut(s) 1181
Bst6I CTCTTC 3 cut(s) 456, 683, 1406
BstACI GRCGYC 1 cut(s) 1514
BstAPI GCANNNNNTGC 2 cut(s) 809, 1320
BstBI TTCGAA 2 cut(s) 1155, 1903
BstC8I GCNNGC 4 cut(s) 171, 814, 1970, 1974
BstDEI CTNAG 7 cut(s) 134, 399, 464, 891, 1093, 1430, 1660
BstDSI CCRYGG 2 cut(s) 680, 1300
BstENI CCTNNNNNAGG 3 cut(s) 541, 647, 1735
BstF5I GGATG 9 cut(s) 619, 690, 874, 914, 1316, 1425, 1609, 1950, 2016
BstFNI CGCG 1 cut(s) 1678
BstH2I RGCGCY 1 cut(s) 1055
BstHHI GCGC 1 cut(s) 1054
BstKTI GATC 9 cut(s) 140, 405, 888, 1017, 1209, 1388, 1555, 1915, 1922
BstMAI GTCTC 4 cut(s) 1167, 1403, 1472, 1704
BstMBI GATC 9 cut(s) 137, 402, 885, 1014, 1206, 1385, 1552, 1912, 1919
BstMCI CGRYCG 1 cut(s) 734
BstMWI GCNNNNNNNGC 9 cut(s) 92, 601, 721, 730, 809, 1089, 1320, 1368, 1965
BstNI CCWGG 5 cut(s) 247, 385, 544, 611, 1917
BstNSI RCATGY 5 cut(s) 45, 254, 812, 879, 992
BstSCI CCNGG 7 cut(s) 26, 245, 383, 542, 609, 955, 1915
BstSLI GKGCMC 1 cut(s) 606
BstUI CGCG 1 cut(s) 1678
BstV1I GCAGC 4 cut(s) 171, 1307, 1907, 1943
BstV2I GAAGAC 2 cut(s) 432, 912
BstX2I RGATCY 3 cut(s) 137, 1912, 1919
BstXI CCANNNNNNTGG 2 cut(s) 232, 1307
BstYI RGATCY 3 cut(s) 137, 1912, 1919
BstZI CGGCCG 1 cut(s) 731
Bsu36I CCTNAGG 1 cut(s) 1660
BsuRI GGCC 6 cut(s) 567, 678, 733, 837, 1533, 1688
BtgI CCRYGG 2 cut(s) 680, 1300
BtgZI GCGATG 1 cut(s) 1974
BtsCI GGATG 9 cut(s) 619, 690, 874, 914, 1316, 1425, 1609, 1950, 2016
BtsIMutI CAGTG 3 cut(s) 111, 1186, 1362
BtuMI TCGCGA 1 cut(s) 1678
Cac8I GCNNGC 4 cut(s) 171, 814, 1970, 1974
CciI TCATGA 2 cut(s) 10, 1347
CfoI GCGC 1 cut(s) 1054
Cfr10I RCCGGY 1 cut(s) 1990
Cfr13I GGNCC 5 cut(s) 242, 677, 771, 983, 1342
CseI GACGC 1 cut(s) 1113
Csp6I GTAC 3 cut(s) 673, 1132, 1327
CspCI CAANNNNNGTGG 2 cut(s) 975, 1010
CviQI GTAC 3 cut(s) 673, 1132, 1327
DdeI CTNAG 7 cut(s) 134, 399, 464, 891, 1093, 1430, 1660
DpnI GATC 9 cut(s) 139, 404, 887, 1016, 1208, 1387, 1554, 1914, 1921
DpnII GATC 9 cut(s) 137, 402, 885, 1014, 1206, 1385, 1552, 1912, 1919
DraI TTTAAA 1 cut(s) 927
EaeI YGGCCR 2 cut(s) 731, 1531
EagI CGGCCG 1 cut(s) 731
Eam1104I CTCTTC 3 cut(s) 456, 683, 1406
EarI CTCTTC 3 cut(s) 456, 683, 1406
EciI GGCGGA 1 cut(s) 1447
EclXI CGGCCG 1 cut(s) 731
Eco130I CCWWGG 5 cut(s) 680, 832, 979, 1300, 1683
Eco147I AGGCCT 1 cut(s) 567
Eco31I GGTCTC 1 cut(s) 1167
Eco47I GGWCC 4 cut(s) 242, 771, 983, 1342
Eco52I CGGCCG 1 cut(s) 731
Eco81I CCTNAGG 1 cut(s) 1660
Eco88I CYCGRG 2 cut(s) 1475, 1735
EcoNI CCTNNNNNAGG 3 cut(s) 541, 647, 1735
EcoO109I RGGNCCY 1 cut(s) 1342
EcoRII CCWGG 5 cut(s) 245, 383, 542, 609, 1915
EcoT14I CCWWGG 5 cut(s) 680, 832, 979, 1300, 1683
EcoT22I ATGCAT 1 cut(s) 805
ErhI CCWWGG 5 cut(s) 680, 832, 979, 1300, 1683
FalI AAGNNNNNCTT 2 cut(s) 1493, 1525
FaqI GGGAC 3 cut(s) 89, 228, 961
FauI CCCGC 1 cut(s) 1961
FbaI TGATCA 1 cut(s) 1552
FblI GTMKAC 2 cut(s) 549, 1224
Fnu4HI GCNGC 8 cut(s) 160, 596, 731, 1321, 1643, 1646, 1896, 1957
FokI GGATG 9 cut(s) 626, 697, 881, 901, 1323, 1412, 1616, 1957, 2023
Fsp4HI GCNGC 8 cut(s) 160, 596, 731, 1321, 1643, 1646, 1896, 1957
FspBI CTAG 6 cut(s) 32, 599, 782, 819, 1568, 1655
GlaI GCGC 1 cut(s) 1053
GluI GCNGC 8 cut(s) 160, 596, 731, 1321, 1643, 1646, 1896, 1957
GsuI CTGGAG 5 cut(s) 71, 254, 437, 1853, 1861
HaeII RGCGCY 1 cut(s) 1055
HaeIII GGCC 6 cut(s) 567, 678, 733, 837, 1533, 1688
HapII CCGG 6 cut(s) 28, 659, 748, 957, 1861, 1991
HgaI GACGC 1 cut(s) 1113
HhaI GCGC 1 cut(s) 1054
Hin1I GRCGYC 1 cut(s) 1514
Hin6I GCGC 1 cut(s) 1052
HinP1I GCGC 1 cut(s) 1052
HincII GTYRAC 2 cut(s) 550, 1144
HindII GTYRAC 2 cut(s) 550, 1144
HinfI GANTC 9 cut(s) 120, 351, 485, 791, 1265, 1480, 1697, 1776, 1835
HpaII CCGG 6 cut(s) 28, 659, 748, 957, 1861, 1991
HphI GGTGA 4 cut(s) 665, 959, 1196, 2005
Hpy166II GTNNAC 5 cut(s) 550, 745, 986, 1144, 1225
Hpy8I GTNNAC 5 cut(s) 550, 745, 986, 1144, 1225
Hpy99I CGWCG 4 cut(s) 739, 1129, 1519, 1678
HpyAV CCTTC 8 cut(s) 142, 151, 466, 508, 557, 890, 1284, 1713
HpyCH4III ACNGT 1 cut(s) 1181
HpyCH4IV ACGT 6 cut(s) 273, 854, 1221, 1294, 1329, 1514
HpyCH4V TGCA 9 cut(s) 86, 446, 621, 803, 812, 1314, 1543, 1587, 2014
HpyF10VI GCNNNNNNNGC 9 cut(s) 92, 601, 721, 730, 809, 1089, 1320, 1368, 1965
HpyF3I CTNAG 7 cut(s) 134, 399, 464, 891, 1093, 1430, 1660
HpySE526I ACGT 6 cut(s) 273, 854, 1221, 1294, 1329, 1514
Hsp92I GRCGYC 1 cut(s) 1514
HspAI GCGC 1 cut(s) 1052
Ksp22I TGATCA 1 cut(s) 1552
Kzo9I GATC 9 cut(s) 137, 402, 885, 1014, 1206, 1385, 1552, 1912, 1919
LmnI GCTCC 1 cut(s) 291
Lsp1109I GCAGC 4 cut(s) 171, 1307, 1907, 1943
LweI GCATC 5 cut(s) 433, 812, 1250, 1301, 2001
MaeI CTAG 6 cut(s) 32, 599, 782, 819, 1568, 1655
MaeII ACGT 6 cut(s) 273, 854, 1221, 1294, 1329, 1514
MaeIII GTNAC 9 cut(s) 572, 581, 871, 965, 1000, 1240, 1295, 1420, 1993
MalI GATC 9 cut(s) 139, 404, 887, 1016, 1208, 1387, 1554, 1914, 1921
MbiI CCGCTC 1 cut(s) 730
MboI GATC 9 cut(s) 137, 402, 885, 1014, 1206, 1385, 1552, 1912, 1919
MboII GAAGA 7 cut(s) 437, 443, 700, 917, 1269, 1393, 1619
MflI RGATCY 3 cut(s) 137, 1912, 1919
MhlI GDGCHC 1 cut(s) 606
MlsI TGGCCA 1 cut(s) 1533
MluCI AATT 9 cut(s) 625, 922, 1072, 1150, 1372, 1407, 1524, 1899, 1905
MluNI TGGCCA 1 cut(s) 1533
MlyI GAGTC 4 cut(s) 345, 1474, 1706, 1844
MmeI TCCRAC 1 cut(s) 798
Mox20I TGGCCA 1 cut(s) 1533
Mph1103I ATGCAT 1 cut(s) 805
MscI TGGCCA 1 cut(s) 1533
MseI TTAA 4 cut(s) 165, 926, 1113, 1563
MslI CAYNNNNRTG 4 cut(s) 612, 944, 1182, 1305
Msp20I TGGCCA 1 cut(s) 1533
MspI CCGG 6 cut(s) 28, 659, 748, 957, 1861, 1991
MspR9I CCNGG 7 cut(s) 28, 247, 385, 544, 611, 957, 1917
Mva1269I GAATGC 2 cut(s) 385, 1871
MvaI CCWGG 5 cut(s) 247, 385, 544, 611, 1917
MvnI CGCG 1 cut(s) 1678
MwoI GCNNNNNNNGC 9 cut(s) 92, 601, 721, 730, 809, 1089, 1320, 1368, 1965
NciI CCSGG 2 cut(s) 28, 957
NcoI CCATGG 2 cut(s) 680, 1300
NdeII GATC 9 cut(s) 137, 402, 885, 1014, 1206, 1385, 1552, 1912, 1919
NlaIV GGNNCC 5 cut(s) 220, 244, 287, 605, 1914
NmuCI GTSAC 7 cut(s) 572, 871, 965, 1240, 1295, 1420, 1993
NruI TCGCGA 1 cut(s) 1678
NsiI ATGCAT 1 cut(s) 805
NspI RCATGY 5 cut(s) 45, 254, 812, 879, 992
NspV TTCGAA 2 cut(s) 1155, 1903
OliI CACNNNNGTG 2 cut(s) 944, 1182
PaeR7I CTCGAG 2 cut(s) 1475, 1735
PagI TCATGA 2 cut(s) 10, 1347
PceI AGGCCT 1 cut(s) 567
PciI ACATGT 1 cut(s) 41
PcsI WCGNNNNNNNCGW 2 cut(s) 1152, 1733
PctI GAATGC 2 cut(s) 385, 1871
PfeI GAWTC 5 cut(s) 120, 485, 791, 1265, 1776
PfoI TCCNGGA 1 cut(s) 1915
PkrI GCNGC 8 cut(s) 161, 597, 732, 1322, 1644, 1647, 1897, 1958
PleI GAGTC 4 cut(s) 345, 1474, 1705, 1843
PpsI GAGTC 4 cut(s) 345, 1474, 1705, 1843
PpuMI RGGWCCY 1 cut(s) 1342
PscI ACATGT 1 cut(s) 41
PsiI TTATAA 1 cut(s) 1599
Psp1406I AACGTT 1 cut(s) 854
Psp5II RGGWCCY 1 cut(s) 1342
Psp6I CCWGG 5 cut(s) 245, 383, 542, 609, 1915
PspGI CCWGG 5 cut(s) 245, 383, 542, 609, 1915
PspN4I GGNNCC 5 cut(s) 220, 244, 287, 605, 1914
PspPI GGNCC 5 cut(s) 242, 677, 771, 983, 1342
PspPPI RGGWCCY 1 cut(s) 1342
PspXI VCTCGAGB 1 cut(s) 1735
PsuI RGATCY 3 cut(s) 137, 1912, 1919
RruI TCGCGA 1 cut(s) 1678
RsaI GTAC 3 cut(s) 674, 1133, 1328
RsaNI GTAC 3 cut(s) 673, 1132, 1327
RseI CAYNNNNRTG 4 cut(s) 612, 944, 1182, 1305
SalI GTCGAC 1 cut(s) 548
SaqAI TTAA 4 cut(s) 165, 926, 1113, 1563
SatI GCNGC 8 cut(s) 160, 596, 731, 1321, 1643, 1646, 1896, 1957
Sau3AI GATC 9 cut(s) 137, 402, 885, 1014, 1206, 1385, 1552, 1912, 1919
Sau96I GGNCC 5 cut(s) 242, 677, 771, 983, 1342
ScaI AGTACT 1 cut(s) 1133
SchI GAGTC 4 cut(s) 345, 1474, 1706, 1844
ScrFI CCNGG 7 cut(s) 28, 247, 385, 544, 611, 957, 1917
SduI GDGCHC 1 cut(s) 606
SfaNI GCATC 5 cut(s) 433, 812, 1250, 1301, 2001
Sfr274I CTCGAG 2 cut(s) 1475, 1735
SfuI TTCGAA 2 cut(s) 1155, 1903
SinI GGWCC 4 cut(s) 242, 771, 983, 1342
SlaI CTCGAG 2 cut(s) 1475, 1735
SmiI ATTTAAAT 1 cut(s) 927
SmiMI CAYNNNNRTG 4 cut(s) 612, 944, 1182, 1305
SmlI CTYRAG 5 cut(s) 644, 725, 1475, 1735, 1846
SmoI CTYRAG 5 cut(s) 644, 725, 1475, 1735, 1846
Sse9I AATT 9 cut(s) 625, 922, 1072, 1150, 1372, 1407, 1524, 1899, 1905
SseBI AGGCCT 1 cut(s) 567
SsiI CCGC 6 cut(s) 595, 730, 1458, 1642, 1645, 1968
SspI AATATT 1 cut(s) 667
SspMI CTAG 6 cut(s) 32, 599, 782, 819, 1568, 1655
StuI AGGCCT 1 cut(s) 567
StyD4I CCNGG 7 cut(s) 26, 245, 383, 542, 609, 955, 1915
StyI CCWWGG 5 cut(s) 680, 832, 979, 1300, 1683
SwaI ATTTAAAT 1 cut(s) 927
TaaI ACNGT 1 cut(s) 1181
TaiI ACGT 6 cut(s) 276, 857, 1224, 1297, 1332, 1517
TaqI TCGA 9 cut(s) 60, 549, 1127, 1155, 1258, 1384, 1476, 1736, 1903
TasI AATT 9 cut(s) 625, 922, 1072, 1150, 1372, 1407, 1524, 1899, 1905
TatI WGTACW 1 cut(s) 1131
TauI GCSGC 4 cut(s) 598, 733, 1645, 1648
TfiI GAWTC 5 cut(s) 120, 485, 791, 1265, 1776
Tru1I TTAA 4 cut(s) 165, 926, 1113, 1563
Tru9I TTAA 4 cut(s) 165, 926, 1113, 1563
TscAI CASTG 3 cut(s) 118, 1186, 1369
TseFI GTSAC 7 cut(s) 572, 871, 965, 1240, 1295, 1420, 1993
TseI GCWGC 4 cut(s) 159, 1320, 1895, 1956
Tsp45I GTSAC 7 cut(s) 572, 871, 965, 1240, 1295, 1420, 1993
TspDTI ATGAA 6 cut(s) 383, 443, 701, 918, 1269, 1620
TspGWI ACGGA 1 cut(s) 1769
TspRI CASTG 3 cut(s) 118, 1186, 1369
VpaK11BI GGWCC 4 cut(s) 242, 771, 983, 1342
XagI CCTNNNNNAGG 3 cut(s) 541, 647, 1735
XapI RAATTY 3 cut(s) 625, 1150, 1905
XceI RCATGY 5 cut(s) 45, 254, 812, 879, 992
XhoI CTCGAG 2 cut(s) 1475, 1735
XmiI GTMKAC 2 cut(s) 549, 1224
XspI CTAG 6 cut(s) 32, 599, 782, 819, 1568, 1655
ZraI GACGTC 1 cut(s) 1515
ZrmI AGTACT 1 cut(s) 1133
Zsp2I ATGCAT 1 cut(s) 805
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.