Rh5DG191300

26S protease regulatory subunit 6B

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000073
Physical Location & Seq
Forward (+)
21236502 .. 21295294
58793 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG191300.1

Sequence Viewer

Length: 1977 bp
ATGCTGGGGTCAGTATTAGAGGTAGAAGATCCTACCATCATTGGGAACAGGGGTTACCTCCGCTTGAGAGTTGATTTCGATATGAGCAAGCCACTCGCAACCTTCATCCAACTCCCCAGAGCAAACTCTTCTACATCCAGAATTCGCCTACAGTACGAGAACCTCCGAAACTTTTGCTTCAATTGTGGCCGCTTGGGGCACATGTTCTCCTCGTGCAGGTATCAAGTTAACCCGATTCTGGTTTCTATGGGAGTTAAGTATGATAATTCCTTGATTGCAGACCCCCCACAGAAACCGGTCTTCACACAATCCAGCTTTCCTGCTGAGTTCCCCTATGTTCCGACAACCGGAATATTTGGCCGGAACAAGAGAGGGTCATTTCAGTCCAAAAATTTGAATTCTGAAAAGTTGGGATCCTCTGAGCACAATGGACCTAACAGGGCACACAATTCAACTTCTGATGGTGACTTGACTGCTAAGTACCATTCAGCAGGCCCATCCTGGTCATTACGTGATAAGTCCCTTTCTCCTTTGAGGCTCCATCGTCCTAGGATCCCTTTGACTGGACCCCCCCACCTTTGGGATCCGGACGCTCATGGCACCTTTTTTAGGAATGGCAGTGTCACCAGAGCTTTGGGTGGATTGAGCATAAAAGATGGGGCTTGGGCTGACCCGGAATGCATTCCTCCATGGGCCTTCCAGAACAAACATGAGTACTACAACTCAAACCCACACTTTCCTGTCCCTACCGAGATAGACAACTTTGGTGGGCCTCTGATTAAGCAAGCTGGACCTCCTTATATTCAGCTAATTGAGTTGAATCTTGCTGACACTATTGTGTCCATGATGGAGGAGAGCACTACTATGCCTGGAGTGACTAAAAGGAAGCGTAAACCAAAGAAATCCCTTGAGGAAGGCGGAGACAGTAAACCAACCCCAAAACGACCCAAAATTCTCCAGAGCCCAAGGCTCAGATTATCTAACACTGGAGTTGAGCAGTATGAAAGAGAGGGTGGATCCCATTGGAATTCTAGTAGAAGGAGCTACTTGAGAACTTTCATCTCTTCTCATTCTCTTCTGGACCCTGGTTTCAAAGGCCAAAGATTTACTTGGGCCAGAGTGGAAGATGGAGTGACGAGGTTACAAGAGAGACTTGACCGCAGCTTGGATATCGGGGGATGCGACATTCAAAAGCAAGAAATCCATGAAGCAGTGGAACTTCCACTAACACACCATGACTTATACAAACAGATTGGAATAGATCCTCCACGTGGTGTATTGCTGTATGGTCCACCTGGAACTGGTAAAATCATGCTAGCTAAGGCTGTTGCCAATCATACAACTACTGCCTTCATTAGAGTTGTTGGTTCAGAATTTGTTCAAAAGTATTTGGGTGAGGTTCCACGAATGGTCCGTGATGTTTTCCATCTCGCCAAGGAGAATGCACCTACCATCATCTTTATTGATGAGGTGGATGCTATTGCTACTGCAAGGTTTGATGCTCAAACTGGAGCTGATAGAAAAGAAAATATTTTTTGTGCAGTACTTCCAGGAATTAATCCAATGATAGTTTGTCCTAAAGTTCTTGAAGCTCAGATATCTCCTAGGTCAAGAAGCTATGCAACTGGTATTTCAAATGTGGCTGAACTTAATAGCCGGAACATGTTCTCAAGCTTAAATAAGAGCCATAGAGCACTTCCTTGGACTTCTAGAAATGATATGACGCTGCGTTTTGCAATGGCTGTCGAGTTGCCAATCTTCTTGAATGATAAAAGGTTGCTATCCACACAAGTAAAAGCACCTGCCCAAGCAAGTGGTTGTAGCTTTGGAGGCGGTGGTTGGTCTCGTGAGAGGTTGAAATTGAGTTCTGGCGACTGTAGATCTGAATCGAACTCTCAAATCAATTTTGTTCTGAGGCAGCATAGGTACCTTTCCTTCAACAATGACGAGGCCAGATCCCCATTCATTCGTTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000502 GO:0003674 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005911 GO:0006355 GO:0006357 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0008134 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009506 GO:0009889 GO:0009891 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010033 GO:0010243 GO:0010468 GO:0010498 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017025 GO:0017111 GO:0019219 GO:0019222 GO:0019538 GO:0019941 GO:0030054 GO:0030162 GO:0030163 GO:0030312 GO:0030433 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031331 GO:0031334 GO:0031595 GO:0031597 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0032991 GO:0033554 GO:0034976 GO:0036402 GO:0036503 GO:0042176 GO:0042221 GO:0042623 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043254 GO:0043632 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0045732 GO:0045862 GO:0045893 GO:0045898 GO:0045899 GO:0045935 GO:0045944 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051603 GO:0051716 GO:0055044 GO:0060255 GO:0060260 GO:0060261 GO:0061136 GO:0065007 GO:0070013 GO:0071704 GO:0071944 GO:0080090 GO:1901564 GO:1901565 GO:1901575 GO:1901698 GO:1901800 GO:1902494 GO:1902680 GO:1903050 GO:1903052 GO:1903362 GO:1903364 GO:1903506 GO:1903508 GO:1905368 GO:1905369 GO:2000112 GO:2000142 GO:2000144 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03050 ko05169 map03050 map05169
Pfam Domains
Protein Families
AAA

Protein Analysis

658

Amino Acids

73.59

Weight (kDa)

9.49

Isoelectric Point (pI)

45.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CCHC_4 PF14392 26 - 74 9e-11 Zinc knuckle
AAA PF00004 426 - 508 1.5e-24 ATPase family associated with various cellular activities (AAA)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000073)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g17821 FvH4_2g04610 FvH4_2g09611 FvH4_2g13563 FvH4_3g21493 FvH4_3g32115 FvH4_3g35060 FvH4_4g00220 FvH4_4g06410 FvH4_4g10367 FvH4_4g10367 FvH4_4g10367 FvH4_5g06101 FvH4_5g08842 FvH4_5g27366 FvH4_5g31283 FvH4_6g11611 FvH4_6g23032 FvH4_7g05661 FvH4_7g06172
malus_domestica MD13G1263700.v1.1
prunus_persica Prupe.1G292300_v2.0.a1 Prupe.5G009700_v2.0.a1 Prupe.6G158100_v2.0.a1 Prupe.8G025100_v2.0.a1 Prupe.8G042600_v2.0.a1
pyrus_communis pycom02g02250 pycom02g16030 pycom05g10490 pycom09g06450 pycom09g06460 pycom13g26200 pycom16g17700
rosa_chinensis RchiOBHm_Chr2g0163321 RchiOBHm_Chr4g0401981 RchiOBHm_Chr5g0005991 RchiOBHm_Chr5g0027191 RchiOBHm_Chr6g0249771
rosa_laevigata RLG00000001040 RLG00000001764 RLG00000002239 RLG00000002240 RLG00000003259 RLG00000008993 RLG00000013847 RLG00000014178 RLG00000015576 RLG00000017269 RLG00000017355 RLG00000018127 RLG00000018602 RLG00000018615 RLG00000019904 RLG00000020044 RLG00000021849 RLG00000021850 RLG00000027965 RLG00000029266 RLG00000029820 RLG00000031112 RLG00000031915 RLG00000033568 RLG00000035013 RLG00000035677 RLG00000036568 RLG00000036628
rosa_multiflora Rmu_co8254085.1_g000001 Rmu_co8462383.1_g000001 Rmu_sc0000185.1_g000049 Rmu_sc0000251.1_g000018 Rmu_sc0000367.1_g000094 Rmu_sc0000498.1_g000023 Rmu_sc0000868.1_g000005 Rmu_sc0001521.1_g000047 Rmu_sc0001534.1_g000007 Rmu_sc0002014.1_g000012 Rmu_sc0003214.1_g000009 Rmu_sc0003573.1_g000041 Rmu_sc0003832.1_g000023 Rmu_sc0004677.1_g000015 Rmu_sc0004967.1_g000014 Rmu_sc0005426.1_g000001 Rmu_sc0005621.1_g000004 Rmu_sc0005640.1_g000009 Rmu_sc0005947.1_g000034 Rmu_sc0006889.1_g000011 Rmu_sc0007121.1_g000014 Rmu_sc0009191.1_g000017 Rmu_sc0010556.1_g000011 Rmu_sc0011396.1_g000007 Rmu_sc0019862.1_g000002 Rmu_sc0022931.1_g000003 Rmu_sc0024164.1_g000001 Rmu_ssc0000391.1_g000027
rosa_roxburghii Rroxscaffold_1G00050060 Rroxscaffold_1G00061810 Rroxscaffold_2G00109590 Rroxscaffold_2G00113810 Rroxscaffold_2G00124040 Rroxscaffold_2G00143620 Rroxscaffold_2G00154760 Rroxscaffold_4G00302820 Rroxscaffold_4G00302830 Rroxscaffold_4G00308680 Rroxscaffold_4G00310240 Rroxscaffold_4G00327890 Rroxscaffold_5G00334600 Rroxscaffold_5G00336050 Rroxscaffold_5G00346560 Rroxscaffold_5G00347860 Rroxscaffold_5G00347870 Rroxscaffold_5G00349330 Rroxscaffold_5G00351980 Rroxscaffold_6G00395280 Rroxscaffold_6G00396750 Rroxscaffold_6G00402910 Rroxscaffold_7G00174790 Rroxscaffold_7G00177570 Rroxscaffold_7G00191820 Rroxscaffold_7G00203480 Rroxscaffold_7G00204380
rosa_rugosa Rorug01G0106600 Rorug01G0106600 Rorug01G0148500.1 Rorug02G0224200 Rorug02G0225500 Rorug02G0233000 Rorug02G0630400 Rorug03G0198000 Rorug03G0232600.1 Rorug03G0240500 Rorug04G0032900 Rorug04G0073300 Rorug04G0118500 Rorug04G0149600.1 Rorug04G0205100 Rorug04G0256700 Rorug05G0020200 Rorug05G0050000 Rorug05G0247300 Rorug05G0295100 Rorug05G0337900 Rorug05G0542600 Rorug06G0034800 Rorug06G0035100 Rorug06G0220100 Rorug07G0091900 Rorug07G0271200
rosa_samantha Rh1AG118200 Rh1AG253000 Rh1AG296300 Rh1AG409000 Rh1AG410600 Rh1BG073400 Rh1BG130400 Rh1BG147300 Rh1BG163600 Rh1CG016000 Rh1DG012200 Rh1DG012300 Rh1DG078600 Rh1DG078800 Rh1DG138100 Rh1DG179200 Rh1DG192600 Rh1DG194500 Rh1DG194600 Rh1DG214300 Rh1DG245900 Rh1DG267000 Rh1DG322700 Rh1DG442700 Rh2AG282000 Rh2AG423000 Rh2AG423100 Rh2AG460700 Rh2AG466200 Rh2AG513400 Rh2AG547000 Rh2BG277800 Rh2BG281200 Rh2BG298300 Rh2BG304300 Rh2BG433500 Rh2CG276000 Rh2CG282600 Rh2CG325500 Rh2CG393000 Rh2DG319200 Rh2DG443400 Rh3AG169300 Rh3AG303600 Rh3BG195000 Rh3BG282600 Rh3BG343400 Rh3BG343500 Rh3BG344100 Rh3CG111700 Rh3CG185700 Rh3CG259100 Rh3CG293000 Rh3CG317900 Rh3CG340900 Rh3CG341000 Rh3CG341100 Rh3CG354700 Rh3CG359200 Rh3CG368100 Rh3DG179900 Rh3DG288700 Rh3DG302600 Rh3DG316000 Rh3DG324500 Rh3DG324600 Rh3DG343200 Rh3DG343300 Rh3DG362400 Rh4AG071800 Rh4BG034000 Rh4BG044300 Rh4BG044400 Rh4BG069600 Rh4BG157400 Rh4CG007000 Rh4CG030400 Rh4CG037900 Rh4CG077700 Rh4CG115000 Rh4CG162400 Rh4CG162500 Rh4CG165500 Rh4CG165600 Rh4CG172900 Rh4CG239700 Rh4DG055900 Rh4DG173200 Rh5AG354900 Rh5AG511100 Rh5BG112600 Rh5BG541000 Rh5BG541100 Rh5BG549300 Rh5CG004400 Rh5CG315000 Rh5CG563800 Rh5CG572100 Rh5DG112200 Rh5DG191300 Rh5DG407600 Rh5DG407700 Rh5DG567500 Rh6AG002600 Rh6AG051400 Rh6AG199800 Rh6AG199900 Rh6AG206200 Rh6AG391000 Rh6AG391100 Rh6BG007500 Rh6BG053400 Rh6BG225900 Rh6BG227100 Rh6BG232400 Rh6CG091100 Rh6CG125400 Rh6CG186700 Rh6CG222700 Rh6CG222800 Rh6CG235500 Rh6CG247200 Rh6CG331200 Rh6CG465100 Rh6DG183500 Rh6DG213500 Rh6DG238800 Rh6DG263700 Rh7AG211800 Rh7AG441800 Rh7BG008600 Rh7BG253000 Rh7BG390000 Rh7CG189600 Rh7CG401500 Rh7CG424200 Rh7CG428700 Rh7CG479800 Rh7CG494300 Rh7DG177000 Rh7DG218700 Rh7DG254000 Rh7DG294800 Rh7DG407200 Rh7DG448800 Rh7DG461500
rosa_wichuraiana Rw0G012180 Rw1G005270 Rw1G012310 Rw1G016890 Rw1G021700 Rw2G014380 Rw2G027690 Rw3G016400 Rw3G028230 Rw3G029320 Rw4G002060 Rw4G002100 Rw4G003980 Rw4G014140 Rw4G014770 Rw4G014780 Rw4G015760 Rw4G021230 Rw5G000520 Rw5G035610 Rw6G001680 Rw6G017890 Rw6G027440 Rw6G034130 Rw6G034170 Rw7G012430 Rw7G033870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1810
Acc36I ACCTGC 2 cut(s) 207, 1810
Acc65I GGTACC 1 cut(s) 1926
AccB1I GGYRCC 2 cut(s) 599, 1926
AccIII TCCGGA 1 cut(s) 586
AciI CCGC 5 cut(s) 61, 190, 918, 1159, 1833
AcoI YGGCCR 2 cut(s) 187, 358
AcsI RAATTY 6 cut(s) 141, 391, 397, 951, 1027, 1373
AcvI CACGTG 1 cut(s) 1271
AdeI CACNNNGTG 1 cut(s) 1274
AfaI GTAC 5 cut(s) 155, 482, 716, 1545, 1928
AflIII ACRYGT 2 cut(s) 201, 1662
AgeI ACCGGT 1 cut(s) 295
AjnI CCWGG 5 cut(s) 500, 868, 1084, 1294, 1549
AleI CACNNNNGTG 1 cut(s) 836
Alw21I GWGCWC 3 cut(s) 426, 860, 1696
Alw26I GTCTC 3 cut(s) 915, 1144, 1848
Aor13HI TCCGGA 1 cut(s) 586
AoxI GGCC 8 cut(s) 187, 358, 493, 693, 770, 1096, 1113, 1950
ApeKI GCWGC 3 cut(s) 1161, 1726, 1918
ApoI RAATTY 6 cut(s) 141, 391, 397, 951, 1027, 1373
AseI ATTAAT 1 cut(s) 1557
AsiGI ACCGGT 1 cut(s) 295
Asp700I GAANNNNTTC 3 cut(s) 681, 1377, 1664
Asp718I GGTACC 1 cut(s) 1926
AspA2I CCTAGG 2 cut(s) 548, 1604
AsuC2I CCSGG 1 cut(s) 674
AsuHPI GGTGA 3 cut(s) 476, 616, 1406
AsuNHI GCTAGC 1 cut(s) 1315
AvaII GGWCC 6 cut(s) 431, 566, 791, 1081, 1289, 1411
AvrII CCTAGG 2 cut(s) 548, 1604
BaeGI GKGCMC 2 cut(s) 201, 445
BamHI GGATCC 4 cut(s) 413, 552, 583, 1016
BanI GGYRCC 2 cut(s) 599, 1926
BanII GRGCYC 1 cut(s) 965
BarI GAAGNNNNNNTAC 2 cut(s) 1919, 1951
BauI CACGAG 2 cut(s) 211, 1845
BbrPI CACGTG 1 cut(s) 1271
BbsI GAAGAC 1 cut(s) 292
Bbv12I GWGCWC 3 cut(s) 426, 860, 1696
BbvI GCAGC 3 cut(s) 1173, 1713, 1930
BccI CCATC 9 cut(s) 44, 455, 505, 549, 650, 841, 1121, 1434, 1460
BcgI CGANNNNNNTGC 4 cut(s) 76, 110, 156, 190
BciT130I CCWGG 5 cut(s) 502, 870, 1086, 1296, 1551
BcnI CCSGG 1 cut(s) 674
BcoDI GTCTC 3 cut(s) 915, 1144, 1848
BfaI CTAG 5 cut(s) 549, 1032, 1316, 1605, 1710
BfmI CTRYAG 2 cut(s) 149, 1876
BfuAI ACCTGC 2 cut(s) 207, 1810
BglII AGATCT 1 cut(s) 1880
BisI GCNGC 4 cut(s) 190, 1162, 1727, 1919
BlnI CCTAGG 2 cut(s) 548, 1604
BlsI GCNGC 4 cut(s) 191, 1163, 1728, 1920
BmcAI AGTACT 2 cut(s) 716, 1545
Bme1390I CCNGG 6 cut(s) 502, 674, 870, 1086, 1296, 1551
Bme18I GGWCC 6 cut(s) 431, 566, 791, 1081, 1289, 1411
BmrFI CCNGG 6 cut(s) 502, 674, 870, 1086, 1296, 1551
BmsI GCATC 3 cut(s) 1169, 1465, 1489
BmtI GCTAGC 1 cut(s) 1319
BpiI GAAGAC 1 cut(s) 292
BpmI CTGGAG 4 cut(s) 891, 941, 1008, 1530
Bpu10I CCTNAGC 1 cut(s) 1320
BpuEI CTTGAG 4 cut(s) 85, 929, 1069, 1654
BpuMI CCSGG 1 cut(s) 674
BsaAI YACGTR 2 cut(s) 512, 1271
BsaBI GATNNNNATC 1 cut(s) 1885
BsaI GGTCTC 1 cut(s) 1848
BsaJI CCNNGG 7 cut(s) 548, 689, 965, 1084, 1434, 1604, 1700
BsaWI WCCGGW 3 cut(s) 295, 347, 586
BsaXI ACNNNNNCTCC 2 cut(s) 191, 221
Bse118I RCCGGY 1 cut(s) 295
Bse1I ACTGG 5 cut(s) 568, 991, 1306, 1513, 1630
Bse3DI GCAATG 1 cut(s) 1743
Bse8I GATNNNNATC 1 cut(s) 1885
BseAI TCCGGA 1 cut(s) 586
BseBI CCWGG 5 cut(s) 502, 870, 1086, 1296, 1551
BseDI CCNNGG 7 cut(s) 548, 689, 965, 1084, 1434, 1604, 1700
BseGI GGATG 5 cut(s) 105, 134, 497, 1184, 1480
BseJI GATNNNNATC 1 cut(s) 1885
BseMI GCAATG 1 cut(s) 1743
BseMII CTCAG 5 cut(s) 315, 411, 985, 1607, 1904
BseNI ACTGG 5 cut(s) 568, 991, 1306, 1513, 1630
BseRI GAGGAG 2 cut(s) 199, 866
BseSI GKGCMC 2 cut(s) 201, 445
BseXI GCAGC 3 cut(s) 1173, 1713, 1930
BseYI CCCAGC 1 cut(s) 4
BsgI GTGCAG 2 cut(s) 235, 1560
BshFI GGCC 8 cut(s) 189, 360, 495, 695, 772, 1098, 1115, 1952
BshNI GGYRCC 2 cut(s) 599, 1926
BshTI ACCGGT 1 cut(s) 295
BsiHKAI GWGCWC 3 cut(s) 426, 860, 1696
BsiSI CCGG 6 cut(s) 296, 348, 361, 587, 674, 1657
BslFI GGGAC 2 cut(s) 505, 728
BsmAI GTCTC 3 cut(s) 915, 1144, 1848
BsmFI GGGAC 2 cut(s) 505, 728
BsmI GAATGC 3 cut(s) 681, 683, 1447
BsnI GGCC 8 cut(s) 189, 360, 495, 695, 772, 1098, 1115, 1952
Bso31I GGTCTC 1 cut(s) 1848
Bsp1286I GDGCHC 6 cut(s) 201, 426, 445, 860, 965, 1696
Bsp13I TCCGGA 1 cut(s) 586
Bsp143I GATC 8 cut(s) 28, 413, 552, 583, 1016, 1261, 1880, 1955
Bsp19I CCATGG 1 cut(s) 689
BspACI CCGC 5 cut(s) 61, 190, 918, 1159, 1833
BspANI GGCC 8 cut(s) 189, 360, 495, 695, 772, 1098, 1115, 1952
BspCNI CTCAG 5 cut(s) 316, 412, 984, 1606, 1905
BspEI TCCGGA 1 cut(s) 586
BspMI ACCTGC 2 cut(s) 207, 1810
BspOI GCTAGC 1 cut(s) 1319
BspT107I GGYRCC 2 cut(s) 599, 1926
BspTNI GGTCTC 1 cut(s) 1848
BsrDI GCAATG 1 cut(s) 1743
BsrFI RCCGGY 1 cut(s) 295
BsrI ACTGG 5 cut(s) 568, 991, 1306, 1513, 1630
BssAI RCCGGY 1 cut(s) 295
BssECI CCNNGG 7 cut(s) 548, 689, 965, 1084, 1434, 1604, 1700
BssMI GATC 8 cut(s) 28, 413, 552, 583, 1016, 1261, 1880, 1955
BssSI CACGAG 2 cut(s) 211, 1845
BssT1I CCWWGG 6 cut(s) 548, 689, 965, 1434, 1604, 1700
Bst2BI CACGAG 2 cut(s) 211, 1845
Bst2UI CCWGG 5 cut(s) 502, 870, 1086, 1296, 1551
Bst4CI ACNGT 3 cut(s) 153, 926, 1877
Bst6I CTCTTC 3 cut(s) 133, 1069, 1080
BstBAI YACGTR 2 cut(s) 512, 1271
BstC8I GCNNGC 4 cut(s) 89, 493, 786, 1317
BstDEI CTNAG 7 cut(s) 324, 420, 477, 971, 1320, 1593, 1913
BstDSI CCRYGG 1 cut(s) 689
BstEII GGTNACC 1 cut(s) 53
BstENI CCTNNNNNAGG 2 cut(s) 214, 607
BstF5I GGATG 5 cut(s) 105, 134, 497, 1184, 1480
BstKTI GATC 8 cut(s) 31, 416, 555, 586, 1019, 1264, 1883, 1958
BstMAI GTCTC 3 cut(s) 915, 1144, 1848
BstMBI GATC 8 cut(s) 28, 413, 552, 583, 1016, 1261, 1880, 1955
BstMWI GCNNNNNNNGC 1 cut(s) 1830
BstNI CCWGG 5 cut(s) 502, 870, 1086, 1296, 1551
BstNSI RCATGY 2 cut(s) 205, 1666
BstPI GGTNACC 1 cut(s) 53
BstSCI CCNGG 6 cut(s) 500, 672, 868, 1084, 1294, 1549
BstSFI CTRYAG 2 cut(s) 149, 1876
BstSLI GKGCMC 2 cut(s) 201, 445
BstV1I GCAGC 3 cut(s) 1173, 1713, 1930
BstV2I GAAGAC 1 cut(s) 292
BstX2I RGATCY 8 cut(s) 28, 413, 552, 583, 1016, 1261, 1880, 1955
BstXI CCANNNNNNTGG 2 cut(s) 634, 1814
BstYI RGATCY 8 cut(s) 28, 413, 552, 583, 1016, 1261, 1880, 1955
BsuRI GGCC 8 cut(s) 189, 360, 495, 695, 772, 1098, 1115, 1952
BtgI CCRYGG 1 cut(s) 689
BtsCI GGATG 5 cut(s) 105, 134, 497, 1184, 1480
BtsI GCAGTG 2 cut(s) 625, 1218
BtsIMutI CAGTG 3 cut(s) 625, 984, 1218
BveI ACCTGC 2 cut(s) 207, 1810
Cac8I GCNNGC 4 cut(s) 89, 493, 786, 1317
Cfr10I RCCGGY 1 cut(s) 295
CseI GACGC 2 cut(s) 599, 1732
Csp6I GTAC 5 cut(s) 154, 481, 715, 1544, 1927
CspAI ACCGGT 1 cut(s) 295
CspCI CAANNNNNGTGG 2 cut(s) 748, 783
CviAII CATG 9 cut(s) 202, 596, 690, 710, 844, 1205, 1235, 1312, 1663
CviQI GTAC 5 cut(s) 154, 481, 715, 1544, 1927
DdeI CTNAG 7 cut(s) 324, 420, 477, 971, 1320, 1593, 1913
DpnI GATC 8 cut(s) 30, 415, 554, 585, 1018, 1263, 1882, 1957
DpnII GATC 8 cut(s) 28, 413, 552, 583, 1016, 1261, 1880, 1955
DraIII CACNNNGTG 1 cut(s) 1274
EaeI YGGCCR 2 cut(s) 187, 358
Eam1104I CTCTTC 3 cut(s) 133, 1069, 1080
EarI CTCTTC 3 cut(s) 133, 1069, 1080
EciI GGCGGA 1 cut(s) 933
Eco130I CCWWGG 6 cut(s) 548, 689, 965, 1434, 1604, 1700
Eco24I GRGCYC 1 cut(s) 965
Eco31I GGTCTC 1 cut(s) 1848
Eco32I GATATC 2 cut(s) 1171, 1599
Eco47I GGWCC 6 cut(s) 431, 566, 791, 1081, 1289, 1411
Eco72I CACGTG 1 cut(s) 1271
Eco91I GGTNACC 1 cut(s) 53
EcoNI CCTNNNNNAGG 2 cut(s) 214, 607
EcoO65I GGTNACC 1 cut(s) 53
EcoRI GAATTC 3 cut(s) 141, 397, 1027
EcoRII CCWGG 5 cut(s) 500, 868, 1084, 1294, 1549
EcoRV GATATC 2 cut(s) 1171, 1599
EcoT14I CCWWGG 6 cut(s) 548, 689, 965, 1434, 1604, 1700
EcoT22I ATGCAT 1 cut(s) 683
EcoT38I GRGCYC 1 cut(s) 965
ErhI CCWWGG 6 cut(s) 548, 689, 965, 1434, 1604, 1700
FaeI CATG 9 cut(s) 205, 599, 693, 713, 847, 1208, 1238, 1315, 1666
FalI AAGNNNNNCTT 4 cut(s) 1093, 1125, 1137, 1169
FaqI GGGAC 2 cut(s) 505, 728
FatI CATG 9 cut(s) 201, 595, 689, 709, 843, 1204, 1234, 1311, 1662
Fnu4HI GCNGC 4 cut(s) 190, 1162, 1727, 1919
FokI GGATG 5 cut(s) 92, 121, 484, 1191, 1487
FriOI GRGCYC 1 cut(s) 965
Fsp4HI GCNGC 4 cut(s) 190, 1162, 1727, 1919
FspBI CTAG 5 cut(s) 549, 1032, 1316, 1605, 1710
GluI GCNGC 4 cut(s) 190, 1162, 1727, 1919
GsaI CCCAGC 1 cut(s) 8
GsuI CTGGAG 4 cut(s) 891, 941, 1008, 1530
HaeIII GGCC 8 cut(s) 189, 360, 495, 695, 772, 1098, 1115, 1952
HapII CCGG 6 cut(s) 296, 348, 361, 587, 674, 1657
HgaI GACGC 2 cut(s) 599, 1732
Hin1II CATG 9 cut(s) 205, 599, 693, 713, 847, 1208, 1238, 1315, 1666
HincII GTYRAC 1 cut(s) 229
HindII GTYRAC 1 cut(s) 229
HindIII AAGCTT 1 cut(s) 1672
HinfI GANTC 3 cut(s) 235, 820, 1886
HpaI GTTAAC 1 cut(s) 229
HpaII CCGG 6 cut(s) 296, 348, 361, 587, 674, 1657
HphI GGTGA 3 cut(s) 476, 616, 1406
Hpy166II GTNNAC 4 cut(s) 229, 893, 929, 1292
Hpy8I GTNNAC 4 cut(s) 229, 893, 929, 1292
HpyAV CCTTC 6 cut(s) 112, 706, 908, 1032, 1360, 1945
HpyCH4III ACNGT 3 cut(s) 153, 926, 1877
HpyCH4IV ACGT 2 cut(s) 511, 1270
HpyCH4V TGCA 8 cut(s) 216, 278, 681, 1445, 1490, 1541, 1622, 1736
HpyF10VI GCNNNNNNNGC 1 cut(s) 1830
HpyF3I CTNAG 7 cut(s) 324, 420, 477, 971, 1320, 1593, 1913
HpySE526I ACGT 2 cut(s) 511, 1270
Hsp92II CATG 9 cut(s) 205, 599, 693, 713, 847, 1208, 1238, 1315, 1666
Kpn2I TCCGGA 1 cut(s) 586
KpnI GGTACC 1 cut(s) 1930
KspAI GTTAAC 1 cut(s) 229
Kzo9I GATC 8 cut(s) 28, 413, 552, 583, 1016, 1261, 1880, 1955
LmnI GCTCC 3 cut(s) 543, 1041, 1511
Lsp1109I GCAGC 3 cut(s) 1173, 1713, 1930
LweI GCATC 3 cut(s) 1169, 1465, 1489
MaeI CTAG 5 cut(s) 549, 1032, 1316, 1605, 1710
MaeII ACGT 2 cut(s) 511, 1270
MaeIII GTNAC 6 cut(s) 53, 464, 622, 874, 1132, 1140
MalI GATC 8 cut(s) 30, 415, 554, 585, 1018, 1263, 1882, 1957
MboI GATC 8 cut(s) 28, 413, 552, 583, 1016, 1261, 1880, 1955
MboII GAAGA 7 cut(s) 38, 120, 292, 1056, 1067, 1136, 1750
MfeI CAATTG 1 cut(s) 181
MflI RGATCY 8 cut(s) 28, 413, 552, 583, 1016, 1261, 1880, 1955
MhlI GDGCHC 6 cut(s) 201, 426, 445, 860, 965, 1696
MmeI TCCRAC 2 cut(s) 133, 365
Mph1103I ATGCAT 1 cut(s) 683
MroI TCCGGA 1 cut(s) 586
MroXI GAANNNNTTC 3 cut(s) 681, 1377, 1664
MseI TTAA 6 cut(s) 228, 255, 780, 1557, 1650, 1676
MslI CAYNNNNRTG 2 cut(s) 836, 863
MspI CCGG 6 cut(s) 296, 348, 361, 587, 674, 1657
MspR9I CCNGG 6 cut(s) 502, 674, 870, 1086, 1296, 1551
MunI CAATTG 1 cut(s) 181
Mva1269I GAATGC 3 cut(s) 681, 683, 1447
MvaI CCWGG 5 cut(s) 502, 870, 1086, 1296, 1551
MwoI GCNNNNNNNGC 1 cut(s) 1830
NciI CCSGG 1 cut(s) 674
NcoI CCATGG 1 cut(s) 689
NdeII GATC 8 cut(s) 28, 413, 552, 583, 1016, 1261, 1880, 1955
NheI GCTAGC 1 cut(s) 1315
NlaIII CATG 9 cut(s) 205, 599, 693, 713, 847, 1208, 1238, 1315, 1666
NmuCI GTSAC 4 cut(s) 464, 622, 874, 1132
NsiI ATGCAT 1 cut(s) 683
NspI RCATGY 2 cut(s) 205, 1666
OliI CACNNNNGTG 1 cut(s) 836
PaqCI CACCTGC 1 cut(s) 1810
PciI ACATGT 2 cut(s) 201, 1662
PcsI WCGNNNNNNNCGW 2 cut(s) 1179, 1411
PctI GAATGC 3 cut(s) 681, 683, 1447
PdmI GAANNNNTTC 3 cut(s) 681, 1377, 1664
PfeI GAWTC 3 cut(s) 235, 820, 1886
PfoI TCCNGGA 1 cut(s) 1549
PinAI ACCGGT 1 cut(s) 295
PkrI GCNGC 4 cut(s) 191, 1163, 1728, 1920
PmaCI CACGTG 1 cut(s) 1271
PmlI CACGTG 1 cut(s) 1271
Ppu21I YACGTR 2 cut(s) 512, 1271
PscI ACATGT 2 cut(s) 201, 1662
PshBI ATTAAT 1 cut(s) 1557
Psp6I CCWGG 5 cut(s) 500, 868, 1084, 1294, 1549
PspCI CACGTG 1 cut(s) 1271
PspEI GGTNACC 1 cut(s) 53
PspFI CCCAGC 1 cut(s) 4
PspGI CCWGG 5 cut(s) 500, 868, 1084, 1294, 1549
PsrI GAACNNNNNNTAC 2 cut(s) 38, 70
PsuI RGATCY 8 cut(s) 28, 413, 552, 583, 1016, 1261, 1880, 1955
RsaI GTAC 5 cut(s) 155, 482, 716, 1545, 1928
RsaNI GTAC 5 cut(s) 154, 481, 715, 1544, 1927
RseI CAYNNNNRTG 2 cut(s) 836, 863
SaqAI TTAA 6 cut(s) 228, 255, 780, 1557, 1650, 1676
SatI GCNGC 4 cut(s) 190, 1162, 1727, 1919
Sau3AI GATC 8 cut(s) 28, 413, 552, 583, 1016, 1261, 1880, 1955
ScaI AGTACT 2 cut(s) 716, 1545
ScrFI CCNGG 6 cut(s) 502, 674, 870, 1086, 1296, 1551
SduI GDGCHC 6 cut(s) 201, 426, 445, 860, 965, 1696
SfaNI GCATC 3 cut(s) 1169, 1465, 1489
SfcI CTRYAG 2 cut(s) 149, 1876
SinI GGWCC 6 cut(s) 431, 566, 791, 1081, 1289, 1411
SmiMI CAYNNNNRTG 2 cut(s) 836, 863
SmlI CTYRAG 4 cut(s) 64, 908, 1048, 1669
SmoI CTYRAG 4 cut(s) 64, 908, 1048, 1669
SsiI CCGC 5 cut(s) 61, 190, 918, 1159, 1833
SspI AATATT 2 cut(s) 354, 1531
SspMI CTAG 5 cut(s) 549, 1032, 1316, 1605, 1710
StyD4I CCNGG 6 cut(s) 500, 672, 868, 1084, 1294, 1549
StyI CCWWGG 6 cut(s) 548, 689, 965, 1434, 1604, 1700
TaaI ACNGT 3 cut(s) 153, 926, 1877
TaiI ACGT 2 cut(s) 514, 1273
TaqI TCGA 3 cut(s) 78, 1746, 1889
TatI WGTACW 2 cut(s) 714, 1543
TauI GCSGC 1 cut(s) 192
TfiI GAWTC 3 cut(s) 235, 820, 1886
Tru1I TTAA 6 cut(s) 228, 255, 780, 1557, 1650, 1676
Tru9I TTAA 6 cut(s) 228, 255, 780, 1557, 1650, 1676
TscAI CASTG 3 cut(s) 625, 991, 1218
TseFI GTSAC 4 cut(s) 464, 622, 874, 1132
TseI GCWGC 3 cut(s) 1161, 1726, 1918
Tsp45I GTSAC 4 cut(s) 464, 622, 874, 1132
TspDTI ATGAA 6 cut(s) 94, 1017, 1048, 1221, 1342, 1954
TspGWI ACGGA 1 cut(s) 1403
TspRI CASTG 3 cut(s) 625, 991, 1218
VpaK11BI GGWCC 6 cut(s) 431, 566, 791, 1081, 1289, 1411
VspI ATTAAT 1 cut(s) 1557
XagI CCTNNNNNAGG 2 cut(s) 214, 607
XapI RAATTY 6 cut(s) 141, 391, 397, 951, 1027, 1373
XbaI TCTAGA 1 cut(s) 1709
XceI RCATGY 2 cut(s) 205, 1666
XmaJI CCTAGG 2 cut(s) 548, 1604
XmnI GAANNNNTTC 3 cut(s) 681, 1377, 1664
XspI CTAG 5 cut(s) 549, 1032, 1316, 1605, 1710
ZrmI AGTACT 2 cut(s) 716, 1545
Zsp2I ATGCAT 1 cut(s) 683
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.