Rroxscaffold_4G00302830

Domain of unknown function (DUF4283)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000073
Physical Location & Seq
Reverse (-)
23167783 .. 23178336
10554 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00302830.1

Sequence Viewer

Length: 651 bp
ATGCATTGTGACCGTGTGGATCACACGAGCTCGGTCACCACTATTTTCGAGGCCACTCATTTTGGTTTGGAGAAGTTGGAGATTGTGGAGTTGGTTTTGATTGAAGTTGATTTTGGTGAATCCCAGGAGCGAAATGGTGGTTTTCAAATGTTCTGCTTGTTTTTCTGTCGAAATTTGAAGCTTCGAATGGGAAATGATCTTCTCCCAACAGCATCTGTTATCAAGGGGAATGAGTGCAAGTTGGTCCTTTCTGATACATCCATGGATTCTCATGACCACTTGGCTGCCAAACTAGAGAACAGTTGTCATATTGACCATGTCAATGACACTGTGTCATTACTGGGTGGCCTTATCGTCGATGTAATGCCAAATATTGGAGGAATTAAAGGTGCTATTCGTACTGCTTGGAAGGACTTTGGTCCACTTCAAATTACTCACATCAGGAAGAACCTTTTCATAATCAGAGTCACTGTAGAAGCTTCCCACAAGATTCTAGAGGGGGGTCCATGGAACATTAATTGTCAACCACCGCTTCAATGTCCTCCGATGGCTTCCAACTTGGTCCATCAACGACGCTCAGTTGCACCTGGTTTCTTTCTGGGTCCAGATTTCTGGACTCACCTTGGAAAAGATGAATGCACACAACGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 zf-CCHC_4 zf-RING_2

Protein Analysis

216

Amino Acids

24.13

Weight (kDa)

6.1

Isoelectric Point (pI)

44.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4283 PF14111 113 - 176 5e-07 Domain of unknown function (DUF4283)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000073)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g17821 FvH4_2g04610 FvH4_2g09611 FvH4_2g13563 FvH4_3g21493 FvH4_3g32115 FvH4_3g35060 FvH4_4g00220 FvH4_4g06410 FvH4_4g10367 FvH4_4g10367 FvH4_4g10367 FvH4_5g06101 FvH4_5g08842 FvH4_5g27366 FvH4_5g31283 FvH4_6g11611 FvH4_6g23032 FvH4_7g05661 FvH4_7g06172
malus_domestica MD13G1263700.v1.1
prunus_persica Prupe.1G292300_v2.0.a1 Prupe.5G009700_v2.0.a1 Prupe.6G158100_v2.0.a1 Prupe.8G025100_v2.0.a1 Prupe.8G042600_v2.0.a1
pyrus_communis pycom02g02250 pycom02g16030 pycom05g10490 pycom09g06450 pycom09g06460 pycom13g26200 pycom16g17700
rosa_chinensis RchiOBHm_Chr2g0163321 RchiOBHm_Chr4g0401981 RchiOBHm_Chr5g0005991 RchiOBHm_Chr5g0027191 RchiOBHm_Chr6g0249771
rosa_laevigata RLG00000001040 RLG00000001764 RLG00000002239 RLG00000002240 RLG00000003259 RLG00000008993 RLG00000013847 RLG00000014178 RLG00000015576 RLG00000017269 RLG00000017355 RLG00000018127 RLG00000018602 RLG00000018615 RLG00000019904 RLG00000020044 RLG00000021849 RLG00000021850 RLG00000027965 RLG00000029266 RLG00000029820 RLG00000031112 RLG00000031915 RLG00000033568 RLG00000035013 RLG00000035677 RLG00000036568 RLG00000036628
rosa_multiflora Rmu_co8254085.1_g000001 Rmu_co8462383.1_g000001 Rmu_sc0000185.1_g000049 Rmu_sc0000251.1_g000018 Rmu_sc0000367.1_g000094 Rmu_sc0000498.1_g000023 Rmu_sc0000868.1_g000005 Rmu_sc0001521.1_g000047 Rmu_sc0001534.1_g000007 Rmu_sc0002014.1_g000012 Rmu_sc0003214.1_g000009 Rmu_sc0003573.1_g000041 Rmu_sc0003832.1_g000023 Rmu_sc0004677.1_g000015 Rmu_sc0004967.1_g000014 Rmu_sc0005426.1_g000001 Rmu_sc0005621.1_g000004 Rmu_sc0005640.1_g000009 Rmu_sc0005947.1_g000034 Rmu_sc0006889.1_g000011 Rmu_sc0007121.1_g000014 Rmu_sc0009191.1_g000017 Rmu_sc0010556.1_g000011 Rmu_sc0011396.1_g000007 Rmu_sc0019862.1_g000002 Rmu_sc0022931.1_g000003 Rmu_sc0024164.1_g000001 Rmu_ssc0000391.1_g000027
rosa_roxburghii Rroxscaffold_1G00050060 Rroxscaffold_1G00061810 Rroxscaffold_2G00109590 Rroxscaffold_2G00113810 Rroxscaffold_2G00124040 Rroxscaffold_2G00143620 Rroxscaffold_2G00154760 Rroxscaffold_4G00302820 Rroxscaffold_4G00302830 Rroxscaffold_4G00308680 Rroxscaffold_4G00310240 Rroxscaffold_4G00327890 Rroxscaffold_5G00334600 Rroxscaffold_5G00336050 Rroxscaffold_5G00346560 Rroxscaffold_5G00347860 Rroxscaffold_5G00347870 Rroxscaffold_5G00349330 Rroxscaffold_5G00351980 Rroxscaffold_6G00395280 Rroxscaffold_6G00396750 Rroxscaffold_6G00402910 Rroxscaffold_7G00174790 Rroxscaffold_7G00177570 Rroxscaffold_7G00191820 Rroxscaffold_7G00203480 Rroxscaffold_7G00204380
rosa_rugosa Rorug01G0106600 Rorug01G0106600 Rorug01G0148500.1 Rorug02G0224200 Rorug02G0225500 Rorug02G0233000 Rorug02G0630400 Rorug03G0198000 Rorug03G0232600.1 Rorug03G0240500 Rorug04G0032900 Rorug04G0073300 Rorug04G0118500 Rorug04G0149600.1 Rorug04G0205100 Rorug04G0256700 Rorug05G0020200 Rorug05G0050000 Rorug05G0247300 Rorug05G0295100 Rorug05G0337900 Rorug05G0542600 Rorug06G0034800 Rorug06G0035100 Rorug06G0220100 Rorug07G0091900 Rorug07G0271200
rosa_samantha Rh1AG118200 Rh1AG253000 Rh1AG296300 Rh1AG409000 Rh1AG410600 Rh1BG073400 Rh1BG130400 Rh1BG147300 Rh1BG163600 Rh1CG016000 Rh1DG012200 Rh1DG012300 Rh1DG078600 Rh1DG078800 Rh1DG138100 Rh1DG179200 Rh1DG192600 Rh1DG194500 Rh1DG194600 Rh1DG214300 Rh1DG245900 Rh1DG267000 Rh1DG322700 Rh1DG442700 Rh2AG282000 Rh2AG423000 Rh2AG423100 Rh2AG460700 Rh2AG466200 Rh2AG513400 Rh2AG547000 Rh2BG277800 Rh2BG281200 Rh2BG298300 Rh2BG304300 Rh2BG433500 Rh2CG276000 Rh2CG282600 Rh2CG325500 Rh2CG393000 Rh2DG319200 Rh2DG443400 Rh3AG169300 Rh3AG303600 Rh3BG195000 Rh3BG282600 Rh3BG343400 Rh3BG343500 Rh3BG344100 Rh3CG111700 Rh3CG185700 Rh3CG259100 Rh3CG293000 Rh3CG317900 Rh3CG340900 Rh3CG341000 Rh3CG341100 Rh3CG354700 Rh3CG359200 Rh3CG368100 Rh3DG179900 Rh3DG288700 Rh3DG302600 Rh3DG316000 Rh3DG324500 Rh3DG324600 Rh3DG343200 Rh3DG343300 Rh3DG362400 Rh4AG071800 Rh4BG034000 Rh4BG044300 Rh4BG044400 Rh4BG069600 Rh4BG157400 Rh4CG007000 Rh4CG030400 Rh4CG037900 Rh4CG077700 Rh4CG115000 Rh4CG162400 Rh4CG162500 Rh4CG165500 Rh4CG165600 Rh4CG172900 Rh4CG239700 Rh4DG055900 Rh4DG173200 Rh5AG354900 Rh5AG511100 Rh5BG112600 Rh5BG541000 Rh5BG541100 Rh5BG549300 Rh5CG004400 Rh5CG315000 Rh5CG563800 Rh5CG572100 Rh5DG112200 Rh5DG191300 Rh5DG407600 Rh5DG407700 Rh5DG567500 Rh6AG002600 Rh6AG051400 Rh6AG199800 Rh6AG199900 Rh6AG206200 Rh6AG391000 Rh6AG391100 Rh6BG007500 Rh6BG053400 Rh6BG225900 Rh6BG227100 Rh6BG232400 Rh6CG091100 Rh6CG125400 Rh6CG186700 Rh6CG222700 Rh6CG222800 Rh6CG235500 Rh6CG247200 Rh6CG331200 Rh6CG465100 Rh6DG183500 Rh6DG213500 Rh6DG238800 Rh6DG263700 Rh7AG211800 Rh7AG441800 Rh7BG008600 Rh7BG253000 Rh7BG390000 Rh7CG189600 Rh7CG401500 Rh7CG424200 Rh7CG428700 Rh7CG479800 Rh7CG494300 Rh7DG177000 Rh7DG218700 Rh7DG254000 Rh7DG294800 Rh7DG407200 Rh7DG448800 Rh7DG461500
rosa_wichuraiana Rw0G012180 Rw1G005270 Rw1G012310 Rw1G016890 Rw1G021700 Rw2G014380 Rw2G027690 Rw3G016400 Rw3G028230 Rw3G029320 Rw4G002060 Rw4G002100 Rw4G003980 Rw4G014140 Rw4G014770 Rw4G014780 Rw4G015760 Rw4G021230 Rw5G000520 Rw5G035610 Rw6G001680 Rw6G017890 Rw6G027440 Rw6G034130 Rw6G034170 Rw7G012430 Rw7G033870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 374
AciI CCGC 1 cut(s) 530
AclWI GGATC 1 cut(s) 27
AcsI RAATTY 1 cut(s) 172
AfaI GTAC 1 cut(s) 400
AfiI CCNNNNNNNGG 1 cut(s) 374
AgsI TTSAA 5 cut(s) 104, 146, 178, 428, 536
AhdI GACNNNNNGTC 1 cut(s) 331
AjnI CCWGG 2 cut(s) 123, 586
AjuI GAANNNNNNNTTGG 2 cut(s) 96, 128
AluBI AGCT 3 cut(s) 30, 181, 479
AluI AGCT 3 cut(s) 30, 181, 479
Alw21I GWGCWC 1 cut(s) 32
AlwI GGATC 1 cut(s) 27
AlwNI CAGNNNCTG 1 cut(s) 215
AoxI GGCC 2 cut(s) 51, 346
ApeKI GCWGC 1 cut(s) 284
ApoI RAATTY 1 cut(s) 172
AseI ATTAAT 1 cut(s) 516
Asp700I GAANNNNTTC 1 cut(s) 452
AspS9I GGNCC 5 cut(s) 244, 419, 503, 562, 602
AsuHPI GGTGA 3 cut(s) 28, 128, 611
AsuII TTCGAA 1 cut(s) 184
AvaII GGWCC 5 cut(s) 244, 419, 503, 562, 602
BanII GRGCYC 1 cut(s) 32
BauI CACGAG 1 cut(s) 25
Bbv12I GWGCWC 1 cut(s) 32
BbvI GCAGC 1 cut(s) 271
BccI CCATC 2 cut(s) 541, 573
BciT130I CCWGG 2 cut(s) 125, 588
BfaI CTAG 2 cut(s) 293, 494
BfmI CTRYAG 1 cut(s) 471
BisI GCNGC 1 cut(s) 285
BlsI GCNGC 1 cut(s) 286
Bme1390I CCNGG 2 cut(s) 125, 588
Bme18I GGWCC 5 cut(s) 244, 419, 503, 562, 602
BmeRI GACNNNNNGTC 1 cut(s) 331
BmgT120I GGNCC 5 cut(s) 244, 419, 503, 562, 602
BmiI GGNNCC 2 cut(s) 504, 603
BmrFI CCNGG 2 cut(s) 125, 588
BmrI ACTGGG 1 cut(s) 350
BmsI GCATC 1 cut(s) 221
BmuI ACTGGG 1 cut(s) 350
BoxI GACNNNNGTC 1 cut(s) 417
Bpu14I TTCGAA 1 cut(s) 184
BsaJI CCNNGG 4 cut(s) 123, 261, 506, 622
Bsc4I CCNNNNNNNGG 1 cut(s) 374
Bse1I ACTGG 1 cut(s) 345
BseBI CCWGG 2 cut(s) 125, 588
BseDI CCNNGG 4 cut(s) 123, 261, 506, 622
BseGI GGATG 1 cut(s) 257
BseLI CCNNNNNNNGG 1 cut(s) 374
BseMII CTCAG 1 cut(s) 591
BseNI ACTGG 1 cut(s) 345
BseXI GCAGC 1 cut(s) 271
BshFI GGCC 2 cut(s) 53, 348
BsiHKAI GWGCWC 1 cut(s) 32
BslI CCNNNNNNNGG 1 cut(s) 374
BsmI GAATGC 1 cut(s) 641
BsnI GGCC 2 cut(s) 53, 348
Bsp119I TTCGAA 1 cut(s) 184
Bsp1286I GDGCHC 1 cut(s) 32
Bsp143I GATC 2 cut(s) 19, 196
Bsp19I CCATGG 2 cut(s) 261, 506
BspACI CCGC 1 cut(s) 530
BspANI GGCC 2 cut(s) 53, 348
BspCNI CTCAG 1 cut(s) 590
BspHI TCATGA 1 cut(s) 271
BspLI GGNNCC 2 cut(s) 504, 603
BspPI GGATC 1 cut(s) 27
BspT104I TTCGAA 1 cut(s) 184
BsrI ACTGG 1 cut(s) 345
BssECI CCNNGG 4 cut(s) 123, 261, 506, 622
BssMI GATC 2 cut(s) 19, 196
BssSI CACGAG 1 cut(s) 25
BssT1I CCWWGG 3 cut(s) 261, 506, 622
Bst2BI CACGAG 1 cut(s) 25
Bst2UI CCWGG 2 cut(s) 125, 588
Bst4CI ACNGT 4 cut(s) 14, 302, 331, 472
BstBI TTCGAA 1 cut(s) 184
BstDEI CTNAG 1 cut(s) 577
BstDSI CCRYGG 2 cut(s) 261, 506
BstEII GGTNACC 1 cut(s) 34
BstF5I GGATG 1 cut(s) 257
BstKTI GATC 2 cut(s) 22, 199
BstMBI GATC 2 cut(s) 19, 196
BstMWI GCNNNNNNNGC 1 cut(s) 645
BstNI CCWGG 2 cut(s) 125, 588
BstPAI GACNNNNGTC 1 cut(s) 417
BstPI GGTNACC 1 cut(s) 34
BstSCI CCNGG 2 cut(s) 123, 586
BstSFI CTRYAG 1 cut(s) 471
BstV1I GCAGC 1 cut(s) 271
BstXI CCANNNNNNTGG 1 cut(s) 612
BsuRI GGCC 2 cut(s) 53, 348
BtgI CCRYGG 2 cut(s) 261, 506
BtsCI GGATG 1 cut(s) 257
BtsIMutI CAGTG 2 cut(s) 327, 468
CaiI CAGNNNCTG 1 cut(s) 215
CciI TCATGA 1 cut(s) 271
Cfr13I GGNCC 5 cut(s) 244, 419, 503, 562, 602
CseI GACGC 1 cut(s) 582
CsiI ACCWGGT 1 cut(s) 586
Csp6I GTAC 1 cut(s) 399
CspCI CAANNNNNGTGG 2 cut(s) 43, 78
CviAII CATG 4 cut(s) 262, 272, 317, 507
CviJI RGCY 7 cut(s) 30, 53, 181, 284, 348, 479, 551
CviKI_1 RGCY 7 cut(s) 30, 53, 181, 284, 348, 479, 551
CviQI GTAC 1 cut(s) 399
DdeI CTNAG 1 cut(s) 577
DpnI GATC 2 cut(s) 21, 198
DpnII GATC 2 cut(s) 19, 196
DriI GACNNNNNGTC 1 cut(s) 331
Eam1105I GACNNNNNGTC 1 cut(s) 331
Ecl136II GAGCTC 1 cut(s) 30
Eco130I CCWWGG 3 cut(s) 261, 506, 622
Eco24I GRGCYC 1 cut(s) 32
Eco47I GGWCC 5 cut(s) 244, 419, 503, 562, 602
Eco53kI GAGCTC 1 cut(s) 30
Eco91I GGTNACC 1 cut(s) 34
EcoICRI GAGCTC 1 cut(s) 30
EcoO65I GGTNACC 1 cut(s) 34
EcoRII CCWGG 2 cut(s) 123, 586
EcoT14I CCWWGG 3 cut(s) 261, 506, 622
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 32
ErhI CCWWGG 3 cut(s) 261, 506, 622
FaeI CATG 4 cut(s) 265, 275, 320, 510
FaiI YATR 6 cut(s) 263, 273, 309, 318, 458, 508
FatI CATG 4 cut(s) 261, 271, 316, 506
Fnu4HI GCNGC 1 cut(s) 285
FokI GGATG 1 cut(s) 244
FriOI GRGCYC 1 cut(s) 32
Fsp4HI GCNGC 1 cut(s) 285
FspBI CTAG 2 cut(s) 293, 494
GluI GCNGC 1 cut(s) 285
HaeIII GGCC 2 cut(s) 53, 348
HgaI GACGC 1 cut(s) 582
Hin1II CATG 4 cut(s) 265, 275, 320, 510
HincII GTYRAC 1 cut(s) 524
HindII GTYRAC 1 cut(s) 524
HindIII AAGCTT 2 cut(s) 179, 477
HinfI GANTC 5 cut(s) 119, 266, 465, 490, 616
HphI GGTGA 3 cut(s) 28, 128, 611
Hpy166II GTNNAC 2 cut(s) 422, 524
Hpy188I TCNGA 3 cut(s) 253, 464, 546
Hpy188III TCNNGA 5 cut(s) 272, 442, 494, 605, 613
Hpy8I GTNNAC 2 cut(s) 422, 524
Hpy99I CGWCG 2 cut(s) 359, 576
HpyAV CCTTC 1 cut(s) 403
HpyCH4III ACNGT 4 cut(s) 14, 302, 331, 472
HpyCH4V TGCA 4 cut(s) 4, 237, 584, 639
HpyF10VI GCNNNNNNNGC 1 cut(s) 645
HpyF3I CTNAG 1 cut(s) 577
Hsp92II CATG 4 cut(s) 265, 275, 320, 510
Kzo9I GATC 2 cut(s) 19, 196
LmnI GCTCC 1 cut(s) 127
LpnPI CCDG 9 cut(s) 110, 137, 326, 427, 573, 584, 598, 600, 618
Lsp1109I GCAGC 1 cut(s) 271
LweI GCATC 1 cut(s) 221
MabI ACCWGGT 1 cut(s) 586
MaeI CTAG 2 cut(s) 293, 494
MaeIII GTNAC 3 cut(s) 8, 34, 466
MalI GATC 2 cut(s) 21, 198
MboI GATC 2 cut(s) 19, 196
MboII GAAGA 2 cut(s) 191, 457
MhlI GDGCHC 1 cut(s) 32
MluCI AATT 4 cut(s) 172, 381, 429, 517
MlyI GAGTC 2 cut(s) 474, 610
MmeI TCCRAC 2 cut(s) 57, 579
MnlI CCTC 4 cut(s) 43, 371, 490, 552
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 1 cut(s) 452
MseI TTAA 2 cut(s) 384, 516
MslI CAYNNNNRTG 1 cut(s) 321
MspR9I CCNGG 2 cut(s) 125, 588
Mva1269I GAATGC 1 cut(s) 641
MvaI CCWGG 2 cut(s) 125, 588
MwoI GCNNNNNNNGC 1 cut(s) 645
NcoI CCATGG 2 cut(s) 261, 506
NdeII GATC 2 cut(s) 19, 196
NlaIII CATG 4 cut(s) 265, 275, 320, 510
NlaIV GGNNCC 2 cut(s) 504, 603
NmuCI GTSAC 3 cut(s) 8, 34, 466
NsiI ATGCAT 1 cut(s) 6
NspV TTCGAA 1 cut(s) 184
PagI TCATGA 1 cut(s) 271
PctI GAATGC 1 cut(s) 641
PdmI GAANNNNTTC 1 cut(s) 452
PfeI GAWTC 3 cut(s) 119, 266, 490
PflFI GACNNNGTC 1 cut(s) 317
PflMI CCANNNNNTGG 1 cut(s) 374
PkrI GCNGC 1 cut(s) 286
PleI GAGTC 2 cut(s) 473, 610
PpsI GAGTC 2 cut(s) 473, 610
PshAI GACNNNNGTC 1 cut(s) 417
PshBI ATTAAT 1 cut(s) 516
Psp124BI GAGCTC 1 cut(s) 32
Psp6I CCWGG 2 cut(s) 123, 586
PspEI GGTNACC 1 cut(s) 34
PspGI CCWGG 2 cut(s) 123, 586
PspN4I GGNNCC 2 cut(s) 504, 603
PspPI GGNCC 5 cut(s) 244, 419, 503, 562, 602
PstNI CAGNNNCTG 1 cut(s) 215
PsyI GACNNNGTC 1 cut(s) 317
RsaI GTAC 1 cut(s) 400
RsaNI GTAC 1 cut(s) 399
RseI CAYNNNNRTG 1 cut(s) 321
SacI GAGCTC 1 cut(s) 32
SaqAI TTAA 2 cut(s) 384, 516
SatI GCNGC 1 cut(s) 285
Sau3AI GATC 2 cut(s) 19, 196
Sau96I GGNCC 5 cut(s) 244, 419, 503, 562, 602
SchI GAGTC 2 cut(s) 474, 610
ScrFI CCNGG 2 cut(s) 125, 588
SduI GDGCHC 1 cut(s) 32
SetI ASST 7 cut(s) 32, 183, 391, 453, 481, 589, 624
SexAI ACCWGGT 1 cut(s) 586
SfaNI GCATC 1 cut(s) 221
SfcI CTRYAG 1 cut(s) 471
SfuI TTCGAA 1 cut(s) 184
SinI GGWCC 5 cut(s) 244, 419, 503, 562, 602
SmiMI CAYNNNNRTG 1 cut(s) 321
Sse9I AATT 4 cut(s) 172, 381, 429, 517
SsiI CCGC 1 cut(s) 530
SspI AATATT 1 cut(s) 373
SspMI CTAG 2 cut(s) 293, 494
SstI GAGCTC 1 cut(s) 32
StyD4I CCNGG 2 cut(s) 123, 586
StyI CCWWGG 3 cut(s) 261, 506, 622
TaaI ACNGT 4 cut(s) 14, 302, 331, 472
TaqI TCGA 4 cut(s) 48, 169, 184, 357
TaqII GACCGA 1 cut(s) 22
TasI AATT 4 cut(s) 172, 381, 429, 517
TfiI GAWTC 3 cut(s) 119, 266, 490
Tru1I TTAA 2 cut(s) 384, 516
Tru9I TTAA 2 cut(s) 384, 516
TscAI CASTG 2 cut(s) 334, 475
TseFI GTSAC 3 cut(s) 8, 34, 466
TseI GCWGC 1 cut(s) 284
Tsp45I GTSAC 3 cut(s) 8, 34, 466
TspDTI ATGAA 2 cut(s) 445, 648
TspRI CASTG 2 cut(s) 334, 475
Tth111I GACNNNGTC 1 cut(s) 317
Van91I CCANNNNNTGG 1 cut(s) 374
VpaK11BI GGWCC 5 cut(s) 244, 419, 503, 562, 602
VspI ATTAAT 1 cut(s) 516
XapI RAATTY 1 cut(s) 172
XbaI TCTAGA 1 cut(s) 493
XcmI CCANNNNNNNNNTGG 1 cut(s) 131
XmnI GAANNNNTTC 1 cut(s) 452
XspI CTAG 2 cut(s) 293, 494
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.