Rorug04G0032900

Domain of unknown function (DUF4283)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0000073
Physical Location & Seq
Reverse (-)
4869466 .. 4871378
1913 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0032900.1

Sequence Viewer

Length: 1512 bp
ATGTCTGCAAATCGTAAAAGTGGTAGAGATCATACAGTTATTGATATTATCGATGACCGCGAGAAAAGCAAGAAATGCAAGAAATGCATCTTCAGAGTTCCTAACGTGCTCCGGAGACAAAACCCAGAAGCATATACACCTGACGTTGTTTCTATTGGACCCTTCCATCATTATCGACAAAAGCGAGGAAAGGAGGACGGTAATGGAGGCAAAGAAGGCAAATGTGAGGAAGATTTCCAACTCATGGAACGAGTGAAAAACAGCTATTTGAATGAAATTCTCGCAGATATGAAGAATGTTACTTTGGAAGAGTTGACTGCAAAAGTTATTGAGCTCTCAGATCAAAAGAATGAAGGTGCCTTTGAGGAACGAGCCCGCAATTTTTATGCAGAACCACTTGATCATATTTCTTCCAAAGACTTCATTGAGATGATGATAGTTGACGGTTGCTTCCTAGTTCAACTGTTTCGGAAGTTTAACGATCCGAAACTCAGGGCCTTTGATGACCCGGTGTTCAACATGGACTGTATGTTTCATTTCCTATGCCACGACATTTTGCTCCTAGAGAATCAACTACCTTGGTTTGTTATCCACATTTTGTATGGCCATATTATCCACAGTTCGCATTGCCATACCCTAGATATAAACCCTGGTGAAGCTTCGAATTCCCTCTCTATTCTCATCCTGAAAGCGCTCAGCATACTACCCTCACTGAAGCATAGTTGCGCAACCTATAAAATGCATCTCAAGAAAAACAAGTGTCATTGTGATGCTCATGTTCTGCACATACTTGATCTGGTAAGAGCTTCCATAGTTATTCCATTAACGACCATAGAAGAGAGAGCGCATAAAGCAACTGATAAAGAAGGTACATCCAGCCCTGAAGAACATCAAGTGCACACTGGGAACCAAGACAAGAGAGACAATAAAGCTGTCCATGAAGACCCAGATCAACATCAAATTCGTACCGCAACCGCACTGTCAAAGGCAGACATTAAATTCGAAAAAGTCGAAAGGGAGAGCTTAATGGACATTCGATTCAACAAGGGTGGGTGGTTCAGGAATGGGATTCTTGAGATTCCACAGCTAAACGTTGGAATGTCATCGGAAACATTATTCAGGAACCTCATTGCAATTGAGCAATGCTACCATGGTTATTCAAACGAGATAACGTCTTATGCCATCTTTATGGATAACCTCATCTCTTCAAATGAGGATATGGAATTACTTTGCAAGGAAAAAGTAATTGGTAACTGGATGAGTGATGAAGATGGTTGTAAGTTCTTCAGCAATCTTTACAAGGACATCCCGCACGAGAAGTTCTACTATGTTGAGCTGTGCGAAAAAGTGAATGATTGTTACAGATCGAAATGGTTTACATGGTTGGCTTTACTCAAGAGTGAAAAATTTTCCAACCCTTGGAGAATTTTGGCTTTCGGGATAGGTATTATCGTTTTGACTCTCACCTCGTGGAACGCGGCAAACAACATTCGGATTCACTGGCACAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 zf-CCHC_4 zf-RING_2

Protein Analysis

503

Amino Acids

58.31

Weight (kDa)

6.42

Isoelectric Point (pI)

46.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 30 - 488 2.5e-100 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000073)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g17821 FvH4_2g04610 FvH4_2g09611 FvH4_2g13563 FvH4_3g21493 FvH4_3g32115 FvH4_3g35060 FvH4_4g00220 FvH4_4g06410 FvH4_4g10367 FvH4_4g10367 FvH4_4g10367 FvH4_5g06101 FvH4_5g08842 FvH4_5g27366 FvH4_5g31283 FvH4_6g11611 FvH4_6g23032 FvH4_7g05661 FvH4_7g06172
malus_domestica MD13G1263700.v1.1
prunus_persica Prupe.1G292300_v2.0.a1 Prupe.5G009700_v2.0.a1 Prupe.6G158100_v2.0.a1 Prupe.8G025100_v2.0.a1 Prupe.8G042600_v2.0.a1
pyrus_communis pycom02g02250 pycom02g16030 pycom05g10490 pycom09g06450 pycom09g06460 pycom13g26200 pycom16g17700
rosa_chinensis RchiOBHm_Chr2g0163321 RchiOBHm_Chr4g0401981 RchiOBHm_Chr5g0005991 RchiOBHm_Chr5g0027191 RchiOBHm_Chr6g0249771
rosa_laevigata RLG00000001040 RLG00000001764 RLG00000002239 RLG00000002240 RLG00000003259 RLG00000008993 RLG00000013847 RLG00000014178 RLG00000015576 RLG00000017269 RLG00000017355 RLG00000018127 RLG00000018602 RLG00000018615 RLG00000019904 RLG00000020044 RLG00000021849 RLG00000021850 RLG00000027965 RLG00000029266 RLG00000029820 RLG00000031112 RLG00000031915 RLG00000033568 RLG00000035013 RLG00000035677 RLG00000036568 RLG00000036628
rosa_multiflora Rmu_co8254085.1_g000001 Rmu_co8462383.1_g000001 Rmu_sc0000185.1_g000049 Rmu_sc0000251.1_g000018 Rmu_sc0000367.1_g000094 Rmu_sc0000498.1_g000023 Rmu_sc0000868.1_g000005 Rmu_sc0001521.1_g000047 Rmu_sc0001534.1_g000007 Rmu_sc0002014.1_g000012 Rmu_sc0003214.1_g000009 Rmu_sc0003573.1_g000041 Rmu_sc0003832.1_g000023 Rmu_sc0004677.1_g000015 Rmu_sc0004967.1_g000014 Rmu_sc0005426.1_g000001 Rmu_sc0005621.1_g000004 Rmu_sc0005640.1_g000009 Rmu_sc0005947.1_g000034 Rmu_sc0006889.1_g000011 Rmu_sc0007121.1_g000014 Rmu_sc0009191.1_g000017 Rmu_sc0010556.1_g000011 Rmu_sc0011396.1_g000007 Rmu_sc0019862.1_g000002 Rmu_sc0022931.1_g000003 Rmu_sc0024164.1_g000001 Rmu_ssc0000391.1_g000027
rosa_roxburghii Rroxscaffold_1G00050060 Rroxscaffold_1G00061810 Rroxscaffold_2G00109590 Rroxscaffold_2G00113810 Rroxscaffold_2G00124040 Rroxscaffold_2G00143620 Rroxscaffold_2G00154760 Rroxscaffold_4G00302820 Rroxscaffold_4G00302830 Rroxscaffold_4G00308680 Rroxscaffold_4G00310240 Rroxscaffold_4G00327890 Rroxscaffold_5G00334600 Rroxscaffold_5G00336050 Rroxscaffold_5G00346560 Rroxscaffold_5G00347860 Rroxscaffold_5G00347870 Rroxscaffold_5G00349330 Rroxscaffold_5G00351980 Rroxscaffold_6G00395280 Rroxscaffold_6G00396750 Rroxscaffold_6G00402910 Rroxscaffold_7G00174790 Rroxscaffold_7G00177570 Rroxscaffold_7G00191820 Rroxscaffold_7G00203480 Rroxscaffold_7G00204380
rosa_rugosa Rorug01G0106600 Rorug01G0106600 Rorug01G0148500.1 Rorug02G0224200 Rorug02G0225500 Rorug02G0233000 Rorug02G0630400 Rorug03G0198000 Rorug03G0232600.1 Rorug03G0240500 Rorug04G0032900 Rorug04G0073300 Rorug04G0118500 Rorug04G0149600.1 Rorug04G0205100 Rorug04G0256700 Rorug05G0020200 Rorug05G0050000 Rorug05G0247300 Rorug05G0295100 Rorug05G0337900 Rorug05G0542600 Rorug06G0034800 Rorug06G0035100 Rorug06G0220100 Rorug07G0091900 Rorug07G0271200
rosa_samantha Rh1AG118200 Rh1AG253000 Rh1AG296300 Rh1AG409000 Rh1AG410600 Rh1BG073400 Rh1BG130400 Rh1BG147300 Rh1BG163600 Rh1CG016000 Rh1DG012200 Rh1DG012300 Rh1DG078600 Rh1DG078800 Rh1DG138100 Rh1DG179200 Rh1DG192600 Rh1DG194500 Rh1DG194600 Rh1DG214300 Rh1DG245900 Rh1DG267000 Rh1DG322700 Rh1DG442700 Rh2AG282000 Rh2AG423000 Rh2AG423100 Rh2AG460700 Rh2AG466200 Rh2AG513400 Rh2AG547000 Rh2BG277800 Rh2BG281200 Rh2BG298300 Rh2BG304300 Rh2BG433500 Rh2CG276000 Rh2CG282600 Rh2CG325500 Rh2CG393000 Rh2DG319200 Rh2DG443400 Rh3AG169300 Rh3AG303600 Rh3BG195000 Rh3BG282600 Rh3BG343400 Rh3BG343500 Rh3BG344100 Rh3CG111700 Rh3CG185700 Rh3CG259100 Rh3CG293000 Rh3CG317900 Rh3CG340900 Rh3CG341000 Rh3CG341100 Rh3CG354700 Rh3CG359200 Rh3CG368100 Rh3DG179900 Rh3DG288700 Rh3DG302600 Rh3DG316000 Rh3DG324500 Rh3DG324600 Rh3DG343200 Rh3DG343300 Rh3DG362400 Rh4AG071800 Rh4BG034000 Rh4BG044300 Rh4BG044400 Rh4BG069600 Rh4BG157400 Rh4CG007000 Rh4CG030400 Rh4CG037900 Rh4CG077700 Rh4CG115000 Rh4CG162400 Rh4CG162500 Rh4CG165500 Rh4CG165600 Rh4CG172900 Rh4CG239700 Rh4DG055900 Rh4DG173200 Rh5AG354900 Rh5AG511100 Rh5BG112600 Rh5BG541000 Rh5BG541100 Rh5BG549300 Rh5CG004400 Rh5CG315000 Rh5CG563800 Rh5CG572100 Rh5DG112200 Rh5DG191300 Rh5DG407600 Rh5DG407700 Rh5DG567500 Rh6AG002600 Rh6AG051400 Rh6AG199800 Rh6AG199900 Rh6AG206200 Rh6AG391000 Rh6AG391100 Rh6BG007500 Rh6BG053400 Rh6BG225900 Rh6BG227100 Rh6BG232400 Rh6CG091100 Rh6CG125400 Rh6CG186700 Rh6CG222700 Rh6CG222800 Rh6CG235500 Rh6CG247200 Rh6CG331200 Rh6CG465100 Rh6DG183500 Rh6DG213500 Rh6DG238800 Rh6DG263700 Rh7AG211800 Rh7AG441800 Rh7BG008600 Rh7BG253000 Rh7BG390000 Rh7CG189600 Rh7CG401500 Rh7CG424200 Rh7CG428700 Rh7CG479800 Rh7CG494300 Rh7DG177000 Rh7DG218700 Rh7DG254000 Rh7DG294800 Rh7DG407200 Rh7DG448800 Rh7DG461500
rosa_wichuraiana Rw0G012180 Rw1G005270 Rw1G012310 Rw1G016890 Rw1G021700 Rw2G014380 Rw2G027690 Rw3G016400 Rw3G028230 Rw3G029320 Rw4G002060 Rw4G002100 Rw4G003980 Rw4G014140 Rw4G014770 Rw4G014780 Rw4G015760 Rw4G021230 Rw5G000520 Rw5G035610 Rw6G001680 Rw6G017890 Rw6G027440 Rw6G034130 Rw6G034170 Rw7G012430 Rw7G033870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 727
AccB1I GGYRCC 1 cut(s) 356
AccB7I CCANNNNNTGG 2 cut(s) 244, 1419
AccII CGCG 2 cut(s) 60, 1478
AccIII TCCGGA 1 cut(s) 111
AciI CCGC 6 cut(s) 58, 376, 969, 975, 1310, 1478
AclI AACGTT 1 cut(s) 1092
AclWI GGATC 1 cut(s) 476
AcoI YGGCCR 1 cut(s) 604
AcsI RAATTY 6 cut(s) 276, 664, 960, 998, 1406, 1425
AcuI CTGAAG 4 cut(s) 76, 734, 903, 1270
AdeI CACNNNGTG 1 cut(s) 1470
AfaI GTAC 2 cut(s) 871, 967
AfeI AGCGCT 1 cut(s) 693
AfiI CCNNNNNNNGG 2 cut(s) 244, 1419
AgsI TTSAA 6 cut(s) 271, 461, 517, 1042, 1161, 1209
AjnI CCWGG 1 cut(s) 649
AjuI GAANNNNNNNTTGG 4 cut(s) 287, 319, 1230, 1262
AluBI AGCT 8 cut(s) 264, 334, 659, 806, 932, 1023, 1087, 1336
AluI AGCT 8 cut(s) 264, 334, 659, 806, 932, 1023, 1087, 1336
Alw21I GWGCWC 3 cut(s) 111, 336, 900
Alw26I GTCTC 2 cut(s) 109, 915
Alw44I GTGCAC 1 cut(s) 896
AlwI GGATC 1 cut(s) 476
Aor13HI TCCGGA 1 cut(s) 111
Aor51HI AGCGCT 1 cut(s) 693
AoxI GGCC 2 cut(s) 495, 604
ApaLI GTGCAC 1 cut(s) 896
ApoI RAATTY 6 cut(s) 276, 664, 960, 998, 1406, 1425
ArsI GACNNNNNNTTYG 2 cut(s) 983, 1015
AspLEI GCGC 3 cut(s) 694, 728, 847
AspS9I GGNCC 2 cut(s) 158, 495
AsuC2I CCSGG 1 cut(s) 509
AsuHPI GGTGA 2 cut(s) 665, 1456
AsuII TTCGAA 2 cut(s) 662, 1002
AvaII GGWCC 1 cut(s) 158
BaeGI GKGCMC 1 cut(s) 900
BalI TGGCCA 1 cut(s) 606
BanI GGYRCC 1 cut(s) 356
BanII GRGCYC 2 cut(s) 336, 376
BauI CACGAG 2 cut(s) 1313, 1468
BbsI GAAGAC 1 cut(s) 948
Bbv12I GWGCWC 3 cut(s) 111, 336, 900
BccI CCATC 3 cut(s) 174, 1190, 1265
BciT130I CCWGG 1 cut(s) 651
BclI TGATCA 1 cut(s) 400
BcnI CCSGG 1 cut(s) 509
BcoDI GTCTC 2 cut(s) 109, 915
BfaI CTAG 3 cut(s) 455, 563, 638
BfoI RGCGCY 1 cut(s) 695
BisI GCNGC 1 cut(s) 1479
BlpI GCTNAGC 1 cut(s) 695
BlsI GCNGC 1 cut(s) 1480
Bme1390I CCNGG 2 cut(s) 509, 651
Bme18I GGWCC 1 cut(s) 158
BmgT120I GGNCC 2 cut(s) 158, 495
BmiI GGNNCC 4 cut(s) 160, 358, 908, 1124
BmrFI CCNGG 2 cut(s) 509, 651
BmrI ACTGGG 1 cut(s) 912
BmsI GCATC 3 cut(s) 96, 751, 760
BmuI ACTGGG 1 cut(s) 912
BpiI GAAGAC 1 cut(s) 948
Bpu1102I GCTNAGC 1 cut(s) 695
Bpu14I TTCGAA 2 cut(s) 662, 1002
BpuEI CTTGAG 3 cut(s) 731, 1094, 1379
BpuMI CCSGG 1 cut(s) 509
Bsa29I ATCGAT 1 cut(s) 51
BsaBI GATNNNNATC 1 cut(s) 954
BsaJI CCNNGG 4 cut(s) 578, 649, 1150, 1418
BsaWI WCCGGW 1 cut(s) 111
Bsc4I CCNNNNNNNGG 2 cut(s) 244, 1419
Bse1I ACTGG 3 cut(s) 907, 1259, 1505
Bse3DI GCAATG 3 cut(s) 625, 1128, 1148
Bse8I GATNNNNATC 1 cut(s) 954
BseAI TCCGGA 1 cut(s) 111
BseBI CCWGG 1 cut(s) 651
BseCI ATCGAT 1 cut(s) 51
BseDI CCNNGG 4 cut(s) 578, 649, 1150, 1418
BseGI GGATG 4 cut(s) 681, 872, 1263, 1305
BseJI GATNNNNATC 1 cut(s) 954
BseLI CCNNNNNNNGG 2 cut(s) 244, 1419
BseMI GCAATG 3 cut(s) 625, 1128, 1148
BseMII CTCAG 3 cut(s) 351, 505, 709
BseNI ACTGG 3 cut(s) 907, 1259, 1505
BseSI GKGCMC 1 cut(s) 900
BsgI GTGCAG 1 cut(s) 767
Bsh1236I CGCG 2 cut(s) 60, 1478
BshFI GGCC 2 cut(s) 497, 606
BshNI GGYRCC 1 cut(s) 356
BshVI ATCGAT 1 cut(s) 51
BsiHKAI GWGCWC 3 cut(s) 111, 336, 900
BsiSI CCGG 2 cut(s) 112, 509
BslI CCNNNNNNNGG 2 cut(s) 244, 1419
BsmAI GTCTC 2 cut(s) 109, 915
BsnI GGCC 2 cut(s) 497, 606
Bsp119I TTCGAA 2 cut(s) 662, 1002
Bsp1286I GDGCHC 4 cut(s) 111, 336, 376, 900
Bsp13I TCCGGA 1 cut(s) 111
Bsp143I GATC 7 cut(s) 28, 340, 400, 481, 793, 949, 1364
Bsp1720I GCTNAGC 1 cut(s) 695
Bsp19I CCATGG 1 cut(s) 1150
BspACI CCGC 6 cut(s) 58, 376, 969, 975, 1310, 1478
BspANI GGCC 2 cut(s) 497, 606
BspCNI CTCAG 3 cut(s) 350, 504, 708
BspDI ATCGAT 1 cut(s) 51
BspEI TCCGGA 1 cut(s) 111
BspFNI CGCG 2 cut(s) 60, 1478
BspLI GGNNCC 4 cut(s) 160, 358, 908, 1124
BspPI GGATC 1 cut(s) 476
BspT104I TTCGAA 2 cut(s) 662, 1002
BspT107I GGYRCC 1 cut(s) 356
BsrDI GCAATG 3 cut(s) 625, 1128, 1148
BsrI ACTGG 3 cut(s) 907, 1259, 1505
BssECI CCNNGG 4 cut(s) 578, 649, 1150, 1418
BssMI GATC 7 cut(s) 28, 340, 400, 481, 793, 949, 1364
BssSI CACGAG 2 cut(s) 1313, 1468
BssT1I CCWWGG 3 cut(s) 578, 1150, 1418
Bst2BI CACGAG 2 cut(s) 1313, 1468
Bst2UI CCWGG 1 cut(s) 651
Bst4CI ACNGT 7 cut(s) 37, 200, 446, 465, 527, 620, 981
Bst6I CTCTTC 3 cut(s) 303, 831, 1210
BstAPI GCANNNNNTGC 2 cut(s) 75, 84
BstBI TTCGAA 2 cut(s) 662, 1002
BstC8I GCNNGC 1 cut(s) 376
BstDEI CTNAG 3 cut(s) 337, 491, 695
BstDSI CCRYGG 1 cut(s) 1150
BstF5I GGATG 4 cut(s) 681, 872, 1263, 1305
BstFNI CGCG 2 cut(s) 60, 1478
BstH2I RGCGCY 1 cut(s) 695
BstHHI GCGC 3 cut(s) 694, 728, 847
BstKTI GATC 7 cut(s) 31, 343, 403, 484, 796, 952, 1367
BstMAI GTCTC 2 cut(s) 109, 915
BstMBI GATC 7 cut(s) 28, 340, 400, 481, 793, 949, 1364
BstMWI GCNNNNNNNGC 5 cut(s) 66, 75, 84, 216, 851
BstNI CCWGG 1 cut(s) 651
BstSCI CCNGG 2 cut(s) 507, 649
BstSLI GKGCMC 1 cut(s) 900
BstUI CGCG 2 cut(s) 60, 1478
BstV2I GAAGAC 1 cut(s) 948
BstXI CCANNNNNNTGG 1 cut(s) 1189
Bsu15I ATCGAT 1 cut(s) 51
BsuRI GGCC 2 cut(s) 497, 606
BsuTUI ATCGAT 1 cut(s) 51
BtgI CCRYGG 1 cut(s) 1150
BtsCI GGATG 4 cut(s) 681, 872, 1263, 1305
BtsIMutI CAGTG 4 cut(s) 710, 900, 977, 1498
Cac8I GCNNGC 1 cut(s) 376
CfoI GCGC 3 cut(s) 694, 728, 847
Cfr13I GGNCC 2 cut(s) 158, 495
ClaI ATCGAT 1 cut(s) 51
Csp6I GTAC 2 cut(s) 870, 966
CspCI CAANNNNNGTGG 2 cut(s) 1030, 1065
CviAII CATG 6 cut(s) 244, 520, 776, 938, 1151, 1380
CviQI GTAC 2 cut(s) 870, 966
DdeI CTNAG 3 cut(s) 337, 491, 695
DpnI GATC 7 cut(s) 30, 342, 402, 483, 795, 951, 1366
DpnII GATC 7 cut(s) 28, 340, 400, 481, 793, 949, 1364
DraIII CACNNNGTG 1 cut(s) 1470
EaeI YGGCCR 1 cut(s) 604
Eam1104I CTCTTC 3 cut(s) 303, 831, 1210
EarI CTCTTC 3 cut(s) 303, 831, 1210
Ecl136II GAGCTC 1 cut(s) 334
Eco130I CCWWGG 3 cut(s) 578, 1150, 1418
Eco24I GRGCYC 2 cut(s) 336, 376
Eco47I GGWCC 1 cut(s) 158
Eco47III AGCGCT 1 cut(s) 693
Eco53kI GAGCTC 1 cut(s) 334
Eco57I CTGAAG 4 cut(s) 76, 734, 903, 1270
EcoICRI GAGCTC 1 cut(s) 334
EcoO109I RGGNCCY 1 cut(s) 495
EcoRI GAATTC 1 cut(s) 664
EcoRII CCWGG 1 cut(s) 649
EcoT14I CCWWGG 3 cut(s) 578, 1150, 1418
EcoT22I ATGCAT 2 cut(s) 89, 744
EcoT38I GRGCYC 2 cut(s) 336, 376
ErhI CCWWGG 3 cut(s) 578, 1150, 1418
FaeI CATG 6 cut(s) 247, 523, 779, 941, 1154, 1383
FatI CATG 6 cut(s) 243, 519, 775, 937, 1150, 1379
FauI CCCGC 2 cut(s) 383, 1317
FbaI TGATCA 1 cut(s) 400
Fnu4HI GCNGC 1 cut(s) 1479
FokI GGATG 4 cut(s) 668, 859, 1270, 1292
FriOI GRGCYC 2 cut(s) 336, 376
Fsp4HI GCNGC 1 cut(s) 1479
FspBI CTAG 3 cut(s) 455, 563, 638
FspI TGCGCA 1 cut(s) 727
GlaI GCGC 3 cut(s) 693, 727, 846
GluI GCNGC 1 cut(s) 1479
HaeII RGCGCY 1 cut(s) 695
HaeIII GGCC 2 cut(s) 497, 606
HapII CCGG 2 cut(s) 112, 509
HhaI GCGC 3 cut(s) 694, 728, 847
Hin1II CATG 6 cut(s) 247, 523, 779, 941, 1154, 1383
Hin6I GCGC 3 cut(s) 692, 726, 845
HinP1I GCGC 3 cut(s) 692, 726, 845
HincII GTYRAC 2 cut(s) 315, 442
HindII GTYRAC 2 cut(s) 315, 442
HindIII AAGCTT 1 cut(s) 657
HinfI GANTC 6 cut(s) 568, 1038, 1069, 1078, 1459, 1495
HpaII CCGG 2 cut(s) 112, 509
HphI GGTGA 2 cut(s) 665, 1456
Hpy166II GTNNAC 4 cut(s) 315, 442, 898, 1377
Hpy188I TCNGA 6 cut(s) 95, 340, 471, 486, 1108, 1494
Hpy188III TCNNGA 8 cut(s) 112, 685, 748, 1060, 1073, 1120, 1396, 1438
Hpy8I GTNNAC 4 cut(s) 315, 442, 898, 1377
HpyAV CCTTC 4 cut(s) 172, 209, 347, 860
HpyCH4III ACNGT 7 cut(s) 37, 200, 446, 465, 527, 620, 981
HpyCH4IV ACGT 4 cut(s) 105, 144, 1092, 1172
HpyF10VI GCNNNNNNNGC 5 cut(s) 66, 75, 84, 216, 851
HpyF3I CTNAG 3 cut(s) 337, 491, 695
HpySE526I ACGT 4 cut(s) 105, 144, 1092, 1172
Hsp92II CATG 6 cut(s) 247, 523, 779, 941, 1154, 1383
HspAI GCGC 3 cut(s) 692, 726, 845
Kpn2I TCCGGA 1 cut(s) 111
Ksp22I TGATCA 1 cut(s) 400
Kzo9I GATC 7 cut(s) 28, 340, 400, 481, 793, 949, 1364
LmnI GCTCC 2 cut(s) 114, 564
LweI GCATC 3 cut(s) 96, 751, 760
MaeI CTAG 3 cut(s) 455, 563, 638
MaeII ACGT 4 cut(s) 105, 144, 1092, 1172
MaeIII GTNAC 3 cut(s) 298, 1250, 1358
MalI GATC 7 cut(s) 30, 342, 402, 483, 795, 951, 1366
MboI GATC 7 cut(s) 28, 340, 400, 481, 793, 949, 1364
MfeI CAATTG 1 cut(s) 1134
MhlI GDGCHC 4 cut(s) 111, 336, 376, 900
MlsI TGGCCA 1 cut(s) 606
MluNI TGGCCA 1 cut(s) 606
MlyI GAGTC 1 cut(s) 1453
MmeI TCCRAC 3 cut(s) 262, 1075, 1437
Mox20I TGGCCA 1 cut(s) 606
Mph1103I ATGCAT 2 cut(s) 89, 744
MroI TCCGGA 1 cut(s) 111
MscI TGGCCA 1 cut(s) 606
MseI TTAA 4 cut(s) 477, 824, 996, 1025
MslI CAYNNNNRTG 3 cut(s) 428, 768, 1187
Msp20I TGGCCA 1 cut(s) 606
MspI CCGG 2 cut(s) 112, 509
MspR9I CCNGG 2 cut(s) 509, 651
MunI CAATTG 1 cut(s) 1134
MvaI CCWGG 1 cut(s) 651
MvnI CGCG 2 cut(s) 60, 1478
MwoI GCNNNNNNNGC 5 cut(s) 66, 75, 84, 216, 851
NciI CCSGG 1 cut(s) 509
NcoI CCATGG 1 cut(s) 1150
NdeII GATC 7 cut(s) 28, 340, 400, 481, 793, 949, 1364
NlaIII CATG 6 cut(s) 247, 523, 779, 941, 1154, 1383
NlaIV GGNNCC 4 cut(s) 160, 358, 908, 1124
NsbI TGCGCA 1 cut(s) 727
NsiI ATGCAT 2 cut(s) 89, 744
NspV TTCGAA 2 cut(s) 662, 1002
PcsI WCGNNNNNNNCGW 3 cut(s) 57, 181, 1008
PfeI GAWTC 5 cut(s) 568, 1038, 1069, 1078, 1495
PflMI CCANNNNNTGG 2 cut(s) 244, 1419
PkrI GCNGC 1 cut(s) 1480
PleI GAGTC 1 cut(s) 1453
PpsI GAGTC 1 cut(s) 1453
Psp124BI GAGCTC 1 cut(s) 336
Psp1406I AACGTT 1 cut(s) 1092
Psp6I CCWGG 1 cut(s) 649
PspGI CCWGG 1 cut(s) 649
PspN4I GGNNCC 4 cut(s) 160, 358, 908, 1124
PspPI GGNCC 2 cut(s) 158, 495
RsaI GTAC 2 cut(s) 871, 967
RsaNI GTAC 2 cut(s) 870, 966
RseI CAYNNNNRTG 3 cut(s) 428, 768, 1187
SacI GAGCTC 1 cut(s) 336
SaqAI TTAA 4 cut(s) 477, 824, 996, 1025
SatI GCNGC 1 cut(s) 1479
Sau3AI GATC 7 cut(s) 28, 340, 400, 481, 793, 949, 1364
Sau96I GGNCC 2 cut(s) 158, 495
SchI GAGTC 1 cut(s) 1453
ScrFI CCNGG 2 cut(s) 509, 651
SduI GDGCHC 4 cut(s) 111, 336, 376, 900
SfaNI GCATC 3 cut(s) 96, 751, 760
SfuI TTCGAA 2 cut(s) 662, 1002
SinI GGWCC 1 cut(s) 158
SmiMI CAYNNNNRTG 3 cut(s) 428, 768, 1187
SmlI CTYRAG 3 cut(s) 746, 1073, 1394
SmoI CTYRAG 3 cut(s) 746, 1073, 1394
SsiI CCGC 6 cut(s) 58, 376, 969, 975, 1310, 1478
SspMI CTAG 3 cut(s) 455, 563, 638
SstI GAGCTC 1 cut(s) 336
StyD4I CCNGG 2 cut(s) 507, 649
StyI CCWWGG 3 cut(s) 578, 1150, 1418
TaaI ACNGT 7 cut(s) 37, 200, 446, 465, 527, 620, 981
TaiI ACGT 4 cut(s) 108, 147, 1095, 1175
TaqI TCGA 7 cut(s) 51, 175, 662, 1002, 1011, 1036, 1367
TauI GCSGC 1 cut(s) 1481
TfiI GAWTC 5 cut(s) 568, 1038, 1069, 1078, 1495
Tru1I TTAA 4 cut(s) 477, 824, 996, 1025
Tru9I TTAA 4 cut(s) 477, 824, 996, 1025
TscAI CASTG 4 cut(s) 717, 907, 984, 1505
TspDTI ATGAA 7 cut(s) 288, 305, 366, 412, 524, 954, 1281
TspRI CASTG 4 cut(s) 717, 907, 984, 1505
Van91I CCANNNNNTGG 2 cut(s) 244, 1419
VneI GTGCAC 1 cut(s) 896
VpaK11BI GGWCC 1 cut(s) 158
XapI RAATTY 6 cut(s) 276, 664, 960, 998, 1406, 1425
XcmI CCANNNNNNNNNTGG 1 cut(s) 599
XspI CTAG 3 cut(s) 455, 563, 638
Zsp2I ATGCAT 2 cut(s) 89, 744
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.