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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 332 |
| AciI | CCGC | 1 cut(s) | 133 |
| AclWI | GGATC | 5 cut(s) | 32, 138, 151, 351, 364 |
| AcsI | RAATTY | 1 cut(s) | 116 |
| AcyI | GRCGYC | 1 cut(s) | 329 |
| AfaI | GTAC | 1 cut(s) | 207 |
| AfiI | CCNNNNNNNGG | 7 cut(s) | 158, 159, 236, 237, 238, 258, 378 |
| AgsI | TTSAA | 2 cut(s) | 71, 187 |
| AjnI | CCWGG | 2 cut(s) | 146, 265 |
| Alw26I | GTCTC | 1 cut(s) | 191 |
| AlwI | GGATC | 5 cut(s) | 32, 138, 151, 351, 364 |
| AoxI | GGCC | 4 cut(s) | 149, 227, 369, 381 |
| ApeKI | GCWGC | 1 cut(s) | 130 |
| ApoI | RAATTY | 1 cut(s) | 116 |
| AspS9I | GGNCC | 5 cut(s) | 150, 228, 262, 305, 381 |
| AsuC2I | CCSGG | 1 cut(s) | 259 |
| AsuHPI | GGTGA | 2 cut(s) | 184, 210 |
| AvaII | GGWCC | 2 cut(s) | 262, 305 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 189, 222 |
| BamHI | GGATCC | 2 cut(s) | 143, 356 |
| BbvI | GCAGC | 1 cut(s) | 117 |
| BccI | CCATC | 1 cut(s) | 223 |
| BciT130I | CCWGG | 2 cut(s) | 148, 267 |
| BcnI | CCSGG | 1 cut(s) | 259 |
| BcoDI | GTCTC | 1 cut(s) | 191 |
| BisI | GCNGC | 1 cut(s) | 131 |
| BlsI | GCNGC | 1 cut(s) | 132 |
| Bme1390I | CCNGG | 3 cut(s) | 148, 259, 267 |
| Bme18I | GGWCC | 2 cut(s) | 262, 305 |
| BmgT120I | GGNCC | 5 cut(s) | 150, 228, 262, 305, 381 |
| BmiI | GGNNCC | 7 cut(s) | 145, 230, 263, 264, 307, 358, 382 |
| BmrFI | CCNGG | 3 cut(s) | 148, 259, 267 |
| BoxI | GACNNNNGTC | 1 cut(s) | 260 |
| BpuEI | CTTGAG | 2 cut(s) | 331, 343 |
| BpuMI | CCSGG | 1 cut(s) | 259 |
| BsaHI | GRCGYC | 1 cut(s) | 329 |
| BsaJI | CCNNGG | 4 cut(s) | 258, 265, 366, 376 |
| Bsc4I | CCNNNNNNNGG | 7 cut(s) | 158, 159, 236, 237, 238, 258, 378 |
| Bse1I | ACTGG | 2 cut(s) | 41, 74 |
| Bse3DI | GCAATG | 1 cut(s) | 105 |
| BseBI | CCWGG | 2 cut(s) | 148, 267 |
| BseDI | CCNNGG | 4 cut(s) | 258, 265, 366, 376 |
| BseLI | CCNNNNNNNGG | 7 cut(s) | 158, 159, 236, 237, 238, 258, 378 |
| BseMI | GCAATG | 1 cut(s) | 105 |
| BseMII | CTCAG | 1 cut(s) | 54 |
| BseNI | ACTGG | 2 cut(s) | 41, 74 |
| BseXI | GCAGC | 1 cut(s) | 117 |
| BshFI | GGCC | 4 cut(s) | 151, 229, 371, 383 |
| BsiSI | CCGG | 1 cut(s) | 258 |
| BslFI | GGGAC | 2 cut(s) | 248, 316 |
| BslI | CCNNNNNNNGG | 7 cut(s) | 158, 159, 236, 237, 238, 258, 378 |
| BsmAI | GTCTC | 1 cut(s) | 191 |
| BsmFI | GGGAC | 2 cut(s) | 248, 316 |
| BsnI | GGCC | 4 cut(s) | 151, 229, 371, 383 |
| Bsp143I | GATC | 3 cut(s) | 37, 143, 356 |
| Bsp19I | CCATGG | 1 cut(s) | 366 |
| BspACI | CCGC | 1 cut(s) | 133 |
| BspANI | GGCC | 4 cut(s) | 151, 229, 371, 383 |
| BspCNI | CTCAG | 1 cut(s) | 55 |
| BspLI | GGNNCC | 7 cut(s) | 145, 230, 263, 264, 307, 358, 382 |
| BspPI | GGATC | 5 cut(s) | 32, 138, 151, 351, 364 |
| BsrDI | GCAATG | 1 cut(s) | 105 |
| BsrI | ACTGG | 2 cut(s) | 41, 74 |
| BssECI | CCNNGG | 4 cut(s) | 258, 265, 366, 376 |
| BssMI | GATC | 3 cut(s) | 37, 143, 356 |
| BssNI | GRCGYC | 1 cut(s) | 329 |
| BssT1I | CCWWGG | 2 cut(s) | 366, 376 |
| Bst2UI | CCWGG | 2 cut(s) | 148, 267 |
| Bst4CI | ACNGT | 1 cut(s) | 288 |
| BstACI | GRCGYC | 1 cut(s) | 329 |
| BstC8I | GCNNGC | 1 cut(s) | 318 |
| BstDEI | CTNAG | 1 cut(s) | 63 |
| BstDSI | CCRYGG | 1 cut(s) | 366 |
| BstKTI | GATC | 3 cut(s) | 40, 146, 359 |
| BstMAI | GTCTC | 1 cut(s) | 191 |
| BstMBI | GATC | 3 cut(s) | 37, 143, 356 |
| BstNI | CCWGG | 2 cut(s) | 148, 267 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 260 |
| BstSCI | CCNGG | 3 cut(s) | 146, 257, 265 |
| BstV1I | GCAGC | 1 cut(s) | 117 |
| BstX2I | RGATCY | 2 cut(s) | 143, 356 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 367 |
| BstYI | RGATCY | 2 cut(s) | 143, 356 |
| BsuRI | GGCC | 4 cut(s) | 151, 229, 371, 383 |
| BtgI | CCRYGG | 1 cut(s) | 366 |
| BtsIMutI | CAGTG | 2 cut(s) | 284, 297 |
| Cac8I | GCNNGC | 1 cut(s) | 318 |
| Cfr13I | GGNCC | 5 cut(s) | 150, 228, 262, 305, 381 |
| Csp6I | GTAC | 1 cut(s) | 206 |
| CviAII | CATG | 3 cut(s) | 154, 276, 367 |
| CviJI | RGCY | 6 cut(s) | 151, 229, 316, 364, 371, 383 |
| CviKI_1 | RGCY | 6 cut(s) | 151, 229, 316, 364, 371, 383 |
| CviQI | GTAC | 1 cut(s) | 206 |
| DdeI | CTNAG | 1 cut(s) | 63 |
| DpnI | GATC | 3 cut(s) | 39, 145, 358 |
| DpnII | GATC | 3 cut(s) | 37, 143, 356 |
| Eco130I | CCWWGG | 2 cut(s) | 366, 376 |
| Eco47I | GGWCC | 2 cut(s) | 262, 305 |
| EcoO109I | RGGNCCY | 1 cut(s) | 262 |
| EcoRII | CCWGG | 2 cut(s) | 146, 265 |
| EcoT14I | CCWWGG | 2 cut(s) | 366, 376 |
| ErhI | CCWWGG | 2 cut(s) | 366, 376 |
| FaeI | CATG | 3 cut(s) | 157, 279, 370 |
| FaiI | YATR | 9 cut(s) | 92, 155, 191, 234, 236, 277, 352, 354, 368 |
| FaqI | GGGAC | 2 cut(s) | 248, 316 |
| FatI | CATG | 3 cut(s) | 153, 275, 366 |
| FauNDI | CATATG | 2 cut(s) | 234, 352 |
| Fnu4HI | GCNGC | 1 cut(s) | 131 |
| Fsp4HI | GCNGC | 1 cut(s) | 131 |
| GluI | GCNGC | 1 cut(s) | 131 |
| HaeIII | GGCC | 4 cut(s) | 151, 229, 371, 383 |
| HapII | CCGG | 1 cut(s) | 258 |
| Hin1I | GRCGYC | 1 cut(s) | 329 |
| Hin1II | CATG | 3 cut(s) | 157, 279, 370 |
| HincII | GTYRAC | 1 cut(s) | 244 |
| HindII | GTYRAC | 1 cut(s) | 244 |
| HpaII | CCGG | 1 cut(s) | 258 |
| HphI | GGTGA | 2 cut(s) | 184, 210 |
| Hpy166II | GTNNAC | 2 cut(s) | 244, 297 |
| Hpy188I | TCNGA | 1 cut(s) | 196 |
| Hpy188III | TCNNGA | 3 cut(s) | 32, 141, 303 |
| Hpy8I | GTNNAC | 2 cut(s) | 244, 297 |
| HpyAV | CCTTC | 1 cut(s) | 382 |
| HpyCH4III | ACNGT | 1 cut(s) | 288 |
| HpyCH4IV | ACGT | 1 cut(s) | 329 |
| HpyCH4V | TGCA | 1 cut(s) | 4 |
| HpyF3I | CTNAG | 1 cut(s) | 63 |
| HpySE526I | ACGT | 1 cut(s) | 329 |
| Hsp92I | GRCGYC | 1 cut(s) | 329 |
| Hsp92II | CATG | 3 cut(s) | 157, 279, 370 |
| KflI | GGGWCCC | 1 cut(s) | 262 |
| Kzo9I | GATC | 3 cut(s) | 37, 143, 356 |
| Lsp1109I | GCAGC | 1 cut(s) | 117 |
| MaeII | ACGT | 1 cut(s) | 329 |
| MaeIII | GTNAC | 1 cut(s) | 280 |
| MalI | GATC | 3 cut(s) | 39, 145, 358 |
| MboI | GATC | 3 cut(s) | 37, 143, 356 |
| MflI | RGATCY | 2 cut(s) | 143, 356 |
| MluCI | AATT | 2 cut(s) | 27, 116 |
| MmeI | TCCRAC | 1 cut(s) | 103 |
| MnlI | CCTC | 7 cut(s) | 62, 168, 306, 310, 318, 346, 352 |
| MspI | CCGG | 1 cut(s) | 258 |
| MspR9I | CCNGG | 3 cut(s) | 148, 259, 267 |
| MvaI | CCWGG | 2 cut(s) | 148, 267 |
| NciI | CCSGG | 1 cut(s) | 259 |
| NcoI | CCATGG | 1 cut(s) | 366 |
| NdeI | CATATG | 2 cut(s) | 234, 352 |
| NdeII | GATC | 3 cut(s) | 37, 143, 356 |
| NlaIII | CATG | 3 cut(s) | 157, 279, 370 |
| NlaIV | GGNNCC | 7 cut(s) | 145, 230, 263, 264, 307, 358, 382 |
| NmuCI | GTSAC | 1 cut(s) | 280 |
| PkrI | GCNGC | 1 cut(s) | 132 |
| PpuMI | RGGWCCY | 1 cut(s) | 262 |
| PshAI | GACNNNNGTC | 1 cut(s) | 260 |
| Psp5II | RGGWCCY | 1 cut(s) | 262 |
| Psp6I | CCWGG | 2 cut(s) | 146, 265 |
| PspGI | CCWGG | 2 cut(s) | 146, 265 |
| PspN4I | GGNNCC | 7 cut(s) | 145, 230, 263, 264, 307, 358, 382 |
| PspPI | GGNCC | 5 cut(s) | 150, 228, 262, 305, 381 |
| PspPPI | RGGWCCY | 1 cut(s) | 262 |
| PsuI | RGATCY | 2 cut(s) | 143, 356 |
| RsaI | GTAC | 1 cut(s) | 207 |
| RsaNI | GTAC | 1 cut(s) | 206 |
| SatI | GCNGC | 1 cut(s) | 131 |
| Sau3AI | GATC | 3 cut(s) | 37, 143, 356 |
| Sau96I | GGNCC | 5 cut(s) | 150, 228, 262, 305, 381 |
| ScrFI | CCNGG | 3 cut(s) | 148, 259, 267 |
| SetI | ASST | 7 cut(s) | 54, 64, 179, 211, 302, 332, 344 |
| SinI | GGWCC | 2 cut(s) | 262, 305 |
| SmlI | CTYRAG | 2 cut(s) | 310, 322 |
| SmoI | CTYRAG | 2 cut(s) | 310, 322 |
| Sse9I | AATT | 2 cut(s) | 27, 116 |
| SsiI | CCGC | 1 cut(s) | 133 |
| StyD4I | CCNGG | 3 cut(s) | 146, 257, 265 |
| StyI | CCWWGG | 2 cut(s) | 366, 376 |
| TaaI | ACNGT | 1 cut(s) | 288 |
| TaiI | ACGT | 1 cut(s) | 332 |
| TaqI | TCGA | 1 cut(s) | 48 |
| TaqII | GACCGA | 1 cut(s) | 261 |
| TasI | AATT | 2 cut(s) | 27, 116 |
| TscAI | CASTG | 2 cut(s) | 291, 297 |
| TseFI | GTSAC | 1 cut(s) | 280 |
| TseI | GCWGC | 1 cut(s) | 130 |
| Tsp45I | GTSAC | 1 cut(s) | 280 |
| TspDTI | ATGAA | 1 cut(s) | 91 |
| TspRI | CASTG | 2 cut(s) | 291, 297 |
| VpaK11BI | GGWCC | 2 cut(s) | 262, 305 |
| XapI | RAATTY | 1 cut(s) | 116 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 222 |
| ZraI | GACGTC | 1 cut(s) | 330 |