Rorug07G0271200

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0000073
Physical Location & Seq
Reverse (-)
25640333 .. 25643577
3245 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0271200.1

Sequence Viewer

Length: 3156 bp
ATGAGATCATTAACTTATAATTATGCTAGAATTTTCACTCTAGCTTGCCTCATCTTGTATGCTCTGCTAGTTTCATCACCAAAAAGAAACGTTGCTTATGCTTCTTCTGCTAGTATTTCTACTGAAGCCGAGGCTCTTCTCAAATGGAAAGCCAGCTTTCGATCCCAAACCCAGAATAATCTTACCTCGTGGACTTTCTTTCCAAAATCCAGTGCCTCTAGTCCATGCAATGTTTGGGTTGGTATTTCATGCAACACTGCTGGAAGTGTCAACAAGATAAATCTCACCAATTCTGGTGTTCAAGGTACGCTGCATGAATTTTCATTCGTAGCTTTCCCTAATCTTGAATATGTTGACCTCAGCTTGAATAAAATATTTGATGTCATCCCACCTCAGATCAGTTCCCTCTCCAAACTTATTTATCTTGATCTATCTTATAATCAGTTGTCTGGGAAAATCCCACCAGAAATTGGTCTTCTAACAAATCTCAAGGTCCTGCACCTTATGGGAAACGAGTTAAATGGCTCAATTCCCCAAGAAATAGGTGACCTTAAGTTTCTCTACGATCTTGCGCTATACACCAACAATCTCGAAGGTTCAATTCCTACATCTCTGGGTAATTTAAGCAACCTGGTTTTTTTTTATCTCTATGAAAACCAACTTTCTGGTCCCATTCCTCCAGAAATGGGAAACCTTCATAATTTGGTTGAGCTCTTTATCGATACCAACCACTTAACAGGTCCCATCCCTCCAAATTTTGGAAATTTCCAAAAGCTCACTGTGATGGCATTGTGGAACAATAATCTTTCTGGTTCTATCCCTTCAGAACTAGGAAATCTAAAGTCTTTGATTAAACTATACATTGACCAGAACAATTATTCTGGTTCAATCCCGGCATCCTTAGGTGACTTGACAAACCTTACACATCTCTGTCTATCTGAAAATAAACTTTCTGGAGCCATTCCGAAAGAGCTGGGGAGCTTGAAATCTCTAGTACATCTAGAATTGCACGTGAATCAACTCAATGGTTCCATTCCAACTTCAATAGGGAACTTGAGAAACTTAGAAATCTTATACCTCCGTGACAACCAACTTTCAGGCTCCATCCCCAAAGAGATGGAGAATCTCATGAAGTTGACTGTACTGGAGTTGGATAGTAACCAATTCTTTGGTAACCTGCCCCAAAATATTTGCCGAGGTGGATCACTAGAAAACTTCACAGCAAACGACAACTATTTGACAGGTCCAATCCCCAAAAGCTTGAAAACTTGCAAGAGCTTATTCAGACTCCGTCTCGAATGGAACCAACTCACAGGCAATATATCTGAAGACTTTGGTGTCTACCCTCGTCTTCAATTTATAGACCTAAGCCACAATAACTTCTATGGTGAAGTCTCACACACTTGGGGACAATGTCCACATTTAGCAACCCTTCGAATTGCGGGAAACAACCTTACTGGTAGCATACCACCTGAGATCAGCAATGCATCTCAAATTCATGAACTCAATCTTTCTTCAAACAGGTTAGTCCGTGCGATTCCAAAGGAGTTTGGGAGAATGACTTCTTTGGTGAAGCTGGTGTTGAATGACAATCAACTGTGGGGTCATATACCCTCAGAGTTTGGATCATTGAATAATCTTGAATACCTCGACCTGTCCACTAACAAATTCAATGAGTCAATTCCAAGCAGTATAGGTAACTTATTCAGATTAAACTACTTGAATTTGAGCAACAACAAGTTGAGTCATGAGATTCCGTTTCAGTCGGGGAAGTTAGTTCACCTGTCTCAACTAGATTTGAGTTACAACTTACTTGAGAATAAGATACCATCAGAACTGAGCAATATGCAAAGCTTGGAGACACTGAATCTTTCTCACAATTATCTTTCTGGTCTCATTCCAACAACTTTTGATGAAATGCCTGGCTTGTTGTACGTTGACATATCTTACAATCAGTTGCAGGGGCCCATTCCAAACAACAAAGCATTTCAAGATGCTACCTTGGATGGGAATGAAGGATTGTGTAGCAATGTTGTTAGAGGACTTCAGCCCTGTAAGAATATCTCAAAATCAAGTCGAAAATCTGTGTTCATAATCACTTTCGTTTCCTCGGGGGCTCTTTCACTTGCTCTCCTTGGAATTGTAATGATTATTAGATCAAGAAAGAAGAAAGAGCATCAACCAATACAGCACGATATGCAGGATGGAGAGATTTTTTCTGTACTTAATTTTGATGGAAGAAAGATGTATGAAGAAATCATAAAAGCAACCAATGGTTTTGACTCCATGTACTGCATTGGTAAAGGAGGATCTGGAAGTGTCTATAAGGCCATGCTATTCTCAGGCGATATAGTTGCAGTAAAGAAACTCCATGGAATACTTGATGGTGATGGCGAGGATGCGGAGACGTCCCTAAAGGACTTCCTCAATGAAGTCAGGGCACTTGTGGAGATACGACACCGAAACATTGTGAAACTTCTTGGTTATTGTTCATATCCAAGTCACTTGTTTTTAATTTATGAGTATCTAGGGAAAGGTAGCTTGTCTTCACTCTTGAGCAACGAACATGAGGCTAAAAATTTAGACTGGAGTTCTAGGGTGAGAATTGTAAAAGGTGTAGCTCATGCATTGTCTTATATGCATCATGATTGCAAGCCACCGATTGTACATCGAGACATATCAAGCAACAACATTTTGCTCAATGACGAATACGAGCCTTGTGTTTCGGACTTTGGGACTGCTAAGCTTCTAAATCCAAACTCATCTAATTGGACGTCCCGTGCAGGCACATATGGATATGTAGCACCAGAGCTTGCTTACACAACGAAGGTAACTGAGAAATCTGATGTTTATAGCTTTGGAGTGCTGGCCCTAGAAGTGCTAATGGGAAAGCAGGCAGGTGATTTCATATCCTCGTTTTCTCAATCGTCTGACAGCGCAAACATATTGCTGAAAGATGTCTTGGATCAACGCCTTCCACATCCGTCACCTCAAGTCGAAGATGAACTGATAACCATTGCAGGGCTAGCAATTGAATGCAGACATTCCCATCCACAATCTAGGCCAACAATGCAAAAGGTTTCCCAGGTCCTATCATCCCGACATGCATATACCTATGGACAGCCAGAGATTACATTTGCACAACTCATTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

1051

Amino Acids

116.57

Weight (kDa)

6.2

Isoelectric Point (pI)

34.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 40 - 85 2.9e-07 Leucine rich repeat N-terminal domain
LRR_8 PF13855 90 - 149 2.1e-06 Leucine rich repeat
LRR_8 PF13855 113 - 173 5.5e-08 Leucine rich repeat
LRR_14 PF23598 176 - 238 1e-05 Leucine-rich repeat region
LRR_14 PF23598 249 - 340 5.6e-09 Leucine-rich repeat region
LRR_14 PF23598 344 - 531 3.2e-09 Leucine-rich repeat region
LRR_14 PF23598 471 - 576 4.2e-06 Leucine-rich repeat region
LRR_8 PF13855 473 - 533 1.9e-06 Leucine rich repeat
LRR_14 PF23598 539 - 626 3.8e-09 Leucine-rich repeat region
LRR_8 PF13855 594 - 653 3.9e-09 Leucine rich repeat
Pkinase PF00069 765 - 1029 1.1e-42 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 766 - 1030 1.8e-39 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000073)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g17821 FvH4_2g04610 FvH4_2g09611 FvH4_2g13563 FvH4_3g21493 FvH4_3g32115 FvH4_3g35060 FvH4_4g00220 FvH4_4g06410 FvH4_4g10367 FvH4_4g10367 FvH4_4g10367 FvH4_5g06101 FvH4_5g08842 FvH4_5g27366 FvH4_5g31283 FvH4_6g11611 FvH4_6g23032 FvH4_7g05661 FvH4_7g06172
malus_domestica MD13G1263700.v1.1
prunus_persica Prupe.1G292300_v2.0.a1 Prupe.5G009700_v2.0.a1 Prupe.6G158100_v2.0.a1 Prupe.8G025100_v2.0.a1 Prupe.8G042600_v2.0.a1
pyrus_communis pycom02g02250 pycom02g16030 pycom05g10490 pycom09g06450 pycom09g06460 pycom13g26200 pycom16g17700
rosa_chinensis RchiOBHm_Chr2g0163321 RchiOBHm_Chr4g0401981 RchiOBHm_Chr5g0005991 RchiOBHm_Chr5g0027191 RchiOBHm_Chr6g0249771
rosa_laevigata RLG00000001040 RLG00000001764 RLG00000002239 RLG00000002240 RLG00000003259 RLG00000008993 RLG00000013847 RLG00000014178 RLG00000015576 RLG00000017269 RLG00000017355 RLG00000018127 RLG00000018602 RLG00000018615 RLG00000019904 RLG00000020044 RLG00000021849 RLG00000021850 RLG00000027965 RLG00000029266 RLG00000029820 RLG00000031112 RLG00000031915 RLG00000033568 RLG00000035013 RLG00000035677 RLG00000036568 RLG00000036628
rosa_multiflora Rmu_co8254085.1_g000001 Rmu_co8462383.1_g000001 Rmu_sc0000185.1_g000049 Rmu_sc0000251.1_g000018 Rmu_sc0000367.1_g000094 Rmu_sc0000498.1_g000023 Rmu_sc0000868.1_g000005 Rmu_sc0001521.1_g000047 Rmu_sc0001534.1_g000007 Rmu_sc0002014.1_g000012 Rmu_sc0003214.1_g000009 Rmu_sc0003573.1_g000041 Rmu_sc0003832.1_g000023 Rmu_sc0004677.1_g000015 Rmu_sc0004967.1_g000014 Rmu_sc0005426.1_g000001 Rmu_sc0005621.1_g000004 Rmu_sc0005640.1_g000009 Rmu_sc0005947.1_g000034 Rmu_sc0006889.1_g000011 Rmu_sc0007121.1_g000014 Rmu_sc0009191.1_g000017 Rmu_sc0010556.1_g000011 Rmu_sc0011396.1_g000007 Rmu_sc0019862.1_g000002 Rmu_sc0022931.1_g000003 Rmu_sc0024164.1_g000001 Rmu_ssc0000391.1_g000027
rosa_roxburghii Rroxscaffold_1G00050060 Rroxscaffold_1G00061810 Rroxscaffold_2G00109590 Rroxscaffold_2G00113810 Rroxscaffold_2G00124040 Rroxscaffold_2G00143620 Rroxscaffold_2G00154760 Rroxscaffold_4G00302820 Rroxscaffold_4G00302830 Rroxscaffold_4G00308680 Rroxscaffold_4G00310240 Rroxscaffold_4G00327890 Rroxscaffold_5G00334600 Rroxscaffold_5G00336050 Rroxscaffold_5G00346560 Rroxscaffold_5G00347860 Rroxscaffold_5G00347870 Rroxscaffold_5G00349330 Rroxscaffold_5G00351980 Rroxscaffold_6G00395280 Rroxscaffold_6G00396750 Rroxscaffold_6G00402910 Rroxscaffold_7G00174790 Rroxscaffold_7G00177570 Rroxscaffold_7G00191820 Rroxscaffold_7G00203480 Rroxscaffold_7G00204380
rosa_rugosa Rorug01G0106600 Rorug01G0106600 Rorug01G0148500.1 Rorug02G0224200 Rorug02G0225500 Rorug02G0233000 Rorug02G0630400 Rorug03G0198000 Rorug03G0232600.1 Rorug03G0240500 Rorug04G0032900 Rorug04G0073300 Rorug04G0118500 Rorug04G0149600.1 Rorug04G0205100 Rorug04G0256700 Rorug05G0020200 Rorug05G0050000 Rorug05G0247300 Rorug05G0295100 Rorug05G0337900 Rorug05G0542600 Rorug06G0034800 Rorug06G0035100 Rorug06G0220100 Rorug07G0091900 Rorug07G0271200
rosa_samantha Rh1AG118200 Rh1AG253000 Rh1AG296300 Rh1AG409000 Rh1AG410600 Rh1BG073400 Rh1BG130400 Rh1BG147300 Rh1BG163600 Rh1CG016000 Rh1DG012200 Rh1DG012300 Rh1DG078600 Rh1DG078800 Rh1DG138100 Rh1DG179200 Rh1DG192600 Rh1DG194500 Rh1DG194600 Rh1DG214300 Rh1DG245900 Rh1DG267000 Rh1DG322700 Rh1DG442700 Rh2AG282000 Rh2AG423000 Rh2AG423100 Rh2AG460700 Rh2AG466200 Rh2AG513400 Rh2AG547000 Rh2BG277800 Rh2BG281200 Rh2BG298300 Rh2BG304300 Rh2BG433500 Rh2CG276000 Rh2CG282600 Rh2CG325500 Rh2CG393000 Rh2DG319200 Rh2DG443400 Rh3AG169300 Rh3AG303600 Rh3BG195000 Rh3BG282600 Rh3BG343400 Rh3BG343500 Rh3BG344100 Rh3CG111700 Rh3CG185700 Rh3CG259100 Rh3CG293000 Rh3CG317900 Rh3CG340900 Rh3CG341000 Rh3CG341100 Rh3CG354700 Rh3CG359200 Rh3CG368100 Rh3DG179900 Rh3DG288700 Rh3DG302600 Rh3DG316000 Rh3DG324500 Rh3DG324600 Rh3DG343200 Rh3DG343300 Rh3DG362400 Rh4AG071800 Rh4BG034000 Rh4BG044300 Rh4BG044400 Rh4BG069600 Rh4BG157400 Rh4CG007000 Rh4CG030400 Rh4CG037900 Rh4CG077700 Rh4CG115000 Rh4CG162400 Rh4CG162500 Rh4CG165500 Rh4CG165600 Rh4CG172900 Rh4CG239700 Rh4DG055900 Rh4DG173200 Rh5AG354900 Rh5AG511100 Rh5BG112600 Rh5BG541000 Rh5BG541100 Rh5BG549300 Rh5CG004400 Rh5CG315000 Rh5CG563800 Rh5CG572100 Rh5DG112200 Rh5DG191300 Rh5DG407600 Rh5DG407700 Rh5DG567500 Rh6AG002600 Rh6AG051400 Rh6AG199800 Rh6AG199900 Rh6AG206200 Rh6AG391000 Rh6AG391100 Rh6BG007500 Rh6BG053400 Rh6BG225900 Rh6BG227100 Rh6BG232400 Rh6CG091100 Rh6CG125400 Rh6CG186700 Rh6CG222700 Rh6CG222800 Rh6CG235500 Rh6CG247200 Rh6CG331200 Rh6CG465100 Rh6DG183500 Rh6DG213500 Rh6DG238800 Rh6DG263700 Rh7AG211800 Rh7AG441800 Rh7BG008600 Rh7BG253000 Rh7BG390000 Rh7CG189600 Rh7CG401500 Rh7CG424200 Rh7CG428700 Rh7CG479800 Rh7CG494300 Rh7DG177000 Rh7DG218700 Rh7DG254000 Rh7DG294800 Rh7DG407200 Rh7DG448800 Rh7DG461500
rosa_wichuraiana Rw0G012180 Rw1G005270 Rw1G012310 Rw1G016890 Rw1G021700 Rw2G014380 Rw2G027690 Rw3G016400 Rw3G028230 Rw3G029320 Rw4G002060 Rw4G002100 Rw4G003980 Rw4G014140 Rw4G014770 Rw4G014780 Rw4G015760 Rw4G021230 Rw5G000520 Rw5G035610 Rw6G001680 Rw6G017890 Rw6G027440 Rw6G034130 Rw6G034170 Rw7G012430 Rw7G033870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 18, 438
AarI CACCTGC 1 cut(s) 2888
AasI GACNNNNNNGTC 1 cut(s) 1337
AatII GACGTC 2 cut(s) 2411, 2777
Acc36I ACCTGC 2 cut(s) 1185, 2888
AccB7I CCANNNNNTGG 2 cut(s) 470, 758
AccI GTMKAC 1 cut(s) 1341
AciI CCGC 2 cut(s) 1442, 2402
AclI AACGTT 1 cut(s) 90
AclWI GGATC 5 cut(s) 156, 1210, 1633, 2317, 2973
AcsI RAATTY 8 cut(s) 30, 317, 754, 763, 1494, 1667, 1723, 2578
AcuI CTGAAG 4 cut(s) 144, 807, 1347, 2030
AcvI CACGTG 1 cut(s) 1012
AcyI GRCGYC 2 cut(s) 2408, 2774
AfaI GTAC 7 cut(s) 307, 996, 1143, 1934, 2223, 2291, 2667
AfiI CCNNNNNNNGG 5 cut(s) 470, 686, 758, 2007, 3021
AflII CTTAAG 1 cut(s) 551
AjnI CCWGG 3 cut(s) 630, 1921, 3084
AjuI GAANNNNNNNTTGG 2 cut(s) 2945, 2977
Alw21I GWGCWC 1 cut(s) 714
Alw26I GTCTC 7 cut(s) 1298, 1399, 1791, 1853, 1898, 2399, 2667
AlwI GGATC 5 cut(s) 156, 1210, 1633, 2317, 2973
Ama87I CYCGRG 1 cut(s) 2110
AoxI GGCC 4 cut(s) 1964, 2328, 2868, 3062
ApaI GGGCCC 1 cut(s) 1968
ApeKI GCWGC 1 cut(s) 310
ApoI RAATTY 8 cut(s) 30, 317, 754, 763, 1494, 1667, 1723, 2578
AseI ATTAAT 1 cut(s) 3150
Asp700I GAANNNNTTC 1 cut(s) 1561
AspLEI GCGC 2 cut(s) 574, 2939
AspS9I GGNCC 8 cut(s) 493, 668, 740, 1244, 1964, 1965, 2869, 3088
AsuC2I CCSGG 1 cut(s) 893
AsuII TTCGAA 1 cut(s) 1435
AsuNHI GCTAGC 1 cut(s) 3025
AvaI CYCGRG 1 cut(s) 2110
AvaII GGWCC 5 cut(s) 493, 668, 740, 1244, 3088
AxyI CCTNAGG 1 cut(s) 901
BaeGI GKGCMC 2 cut(s) 1968, 2443
BanII GRGCYC 3 cut(s) 714, 1968, 2119
BarI GAAGNNNNNNTAC 2 cut(s) 2530, 2562
BauI CACGAG 1 cut(s) 187
BbrPI CACGTG 1 cut(s) 1012
BbsI GAAGAC 4 cut(s) 467, 1335, 1343, 2538
Bbv12I GWGCWC 1 cut(s) 714
BbvCI CCTCAGC 1 cut(s) 359
BbvI GCAGC 1 cut(s) 297
BcgI CGANNNNNNTGC 2 cut(s) 2336, 2370
BciT130I CCWGG 3 cut(s) 632, 1923, 3086
BcnI CCSGG 1 cut(s) 893
BcoDI GTCTC 7 cut(s) 1298, 1399, 1791, 1853, 1898, 2399, 2667
BfrI CTTAAG 1 cut(s) 551
BfuAI ACCTGC 2 cut(s) 1185, 2888
BisI GCNGC 1 cut(s) 311
BlpI GCTNAGC 1 cut(s) 2742
BlsI GCNGC 1 cut(s) 312
Bme1390I CCNGG 4 cut(s) 632, 893, 1923, 3086
Bme18I GGWCC 5 cut(s) 493, 668, 740, 1244, 3088
BmeT110I CYCGRG 1 cut(s) 2110
BmgT120I GGNCC 8 cut(s) 493, 668, 740, 1244, 1964, 1965, 2869, 3088
BmiI GGNNCC 8 cut(s) 670, 742, 958, 1030, 1102, 1304, 1965, 1966
BmrFI CCNGG 4 cut(s) 632, 893, 1923, 3086
BmsI GCATC 6 cut(s) 905, 1496, 1984, 2185, 2389, 2650
BmtI GCTAGC 1 cut(s) 3029
BpiI GAAGAC 4 cut(s) 467, 1335, 1343, 2538
BpmI CTGGAG 4 cut(s) 663, 975, 1166, 2608
Bpu10I CCTNAGC 2 cut(s) 359, 1367
Bpu1102I GCTNAGC 1 cut(s) 2742
Bpu14I TTCGAA 1 cut(s) 1435
BpuEI CTTGAG 5 cut(s) 473, 1075, 1835, 2575, 2976
BpuMI CCSGG 1 cut(s) 893
Bsa29I ATCGAT 1 cut(s) 720
BsaAI YACGTR 1 cut(s) 1012
BsaHI GRCGYC 2 cut(s) 2408, 2774
BsaI GGTCTC 1 cut(s) 1898
BsaJI CCNNGG 7 cut(s) 129, 1195, 2001, 2109, 2134, 2371, 3084
BsaXI ACNNNNNCTCC 2 cut(s) 2115, 2145
Bsc4I CCNNNNNNNGG 5 cut(s) 470, 686, 758, 2007, 3021
Bse1I ACTGG 4 cut(s) 210, 1149, 1462, 2591
Bse21I CCTNAGG 1 cut(s) 901
Bse3DI GCAATG 4 cut(s) 235, 1489, 2035, 3015
BseBI CCWGG 3 cut(s) 632, 1923, 3086
BseCI ATCGAT 1 cut(s) 720
BseDI CCNNGG 7 cut(s) 129, 1195, 2001, 2109, 2134, 2371, 3084
BseLI CCNNNNNNNGG 5 cut(s) 470, 686, 758, 2007, 3021
BseMI GCAATG 4 cut(s) 235, 1489, 2035, 3015
BseMII CTCAG 7 cut(s) 373, 407, 1464, 1629, 1829, 2355, 2826
BseNI ACTGG 4 cut(s) 210, 1149, 1462, 2591
BseSI GKGCMC 2 cut(s) 1968, 2443
BseXI GCAGC 1 cut(s) 297
BseYI CCCAGC 1 cut(s) 973
BsgI GTGCAG 2 cut(s) 482, 2802
BshFI GGCC 4 cut(s) 1966, 2330, 2870, 3064
BshVI ATCGAT 1 cut(s) 720
BsiHKAI GWGCWC 1 cut(s) 714
BsiHKCI CYCGRG 1 cut(s) 2110
BsiSI CCGG 1 cut(s) 893
BslFI GGGAC 6 cut(s) 654, 726, 1422, 2395, 2749, 2761
BslI CCNNNNNNNGG 5 cut(s) 470, 686, 758, 2007, 3021
BsmAI GTCTC 7 cut(s) 1298, 1399, 1791, 1853, 1898, 2399, 2667
BsmBI CGTCTC 2 cut(s) 1298, 2399
BsmFI GGGAC 6 cut(s) 654, 726, 1422, 2395, 2749, 2761
BsmI GAATGC 1 cut(s) 3041
BsnI GGCC 4 cut(s) 1966, 2330, 2870, 3064
Bso31I GGTCTC 1 cut(s) 1898
BsoBI CYCGRG 1 cut(s) 2110
Bsp119I TTCGAA 1 cut(s) 1435
Bsp120I GGGCCC 1 cut(s) 1964
Bsp1286I GDGCHC 4 cut(s) 714, 1968, 2119, 2443
Bsp1407I TGTACA 1 cut(s) 2665
Bsp1720I GCTNAGC 1 cut(s) 2742
Bsp19I CCATGG 1 cut(s) 2371
BspACI CCGC 2 cut(s) 1442, 2402
BspANI GGCC 4 cut(s) 1966, 2330, 2870, 3064
BspCNI CTCAG 7 cut(s) 372, 406, 1465, 1628, 1830, 2354, 2827
BspDI ATCGAT 1 cut(s) 720
BspHI TCATGA 4 cut(s) 1128, 1498, 1747, 2644
BspLI GGNNCC 8 cut(s) 670, 742, 958, 1030, 1102, 1304, 1965, 1966
BspMI ACCTGC 2 cut(s) 1185, 2888
BspOI GCTAGC 1 cut(s) 3029
BspPI GGATC 5 cut(s) 156, 1210, 1633, 2317, 2973
BspQI GCTCTTC 1 cut(s) 141
BspT104I TTCGAA 1 cut(s) 1435
BspTI CTTAAG 1 cut(s) 551
BspTNI GGTCTC 1 cut(s) 1898
BsrDI GCAATG 4 cut(s) 235, 1489, 2035, 3015
BsrGI TGTACA 1 cut(s) 2665
BsrI ACTGG 4 cut(s) 210, 1149, 1462, 2591
BssECI CCNNGG 7 cut(s) 129, 1195, 2001, 2109, 2134, 2371, 3084
BssNI GRCGYC 2 cut(s) 2408, 2774
BssSI CACGAG 1 cut(s) 187
BssT1I CCWWGG 3 cut(s) 2001, 2134, 2371
Bst2BI CACGAG 1 cut(s) 187
Bst2UI CCWGG 3 cut(s) 632, 1923, 3086
Bst4CI ACNGT 3 cut(s) 781, 1141, 1599
Bst6I CTCTTC 1 cut(s) 141
BstACI GRCGYC 2 cut(s) 2408, 2774
BstAFI CTTAAG 1 cut(s) 551
BstAPI GCANNNNNTGC 1 cut(s) 2197
BstAUI TGTACA 1 cut(s) 2665
BstBAI YACGTR 1 cut(s) 1012
BstBI TTCGAA 1 cut(s) 1435
BstC8I GCNNGC 8 cut(s) 46, 154, 2654, 2785, 2814, 2868, 2895, 3027
BstDSI CCRYGG 1 cut(s) 2371
BstEII GGTNACC 2 cut(s) 545, 1172
BstHHI GCGC 2 cut(s) 574, 2939
BstMAI GTCTC 7 cut(s) 1298, 1399, 1791, 1853, 1898, 2399, 2667
BstMWI GCNNNNNNNGC 4 cut(s) 107, 2197, 3026, 3070
BstNI CCWGG 3 cut(s) 632, 1923, 3086
BstNSI RCATGY 1 cut(s) 3107
BstPI GGTNACC 2 cut(s) 545, 1172
BstSCI CCNGG 4 cut(s) 630, 891, 1921, 3084
BstSLI GKGCMC 2 cut(s) 1968, 2443
BstV1I GCAGC 1 cut(s) 297
BstV2I GAAGAC 4 cut(s) 467, 1335, 1343, 2538
BstX2I RGATCY 1 cut(s) 2309
BstXI CCANNNNNNTGG 3 cut(s) 665, 1117, 1169
BstYI RGATCY 1 cut(s) 2309
Bsu15I ATCGAT 1 cut(s) 720
Bsu36I CCTNAGG 1 cut(s) 901
BsuRI GGCC 4 cut(s) 1966, 2330, 2870, 3064
BsuTUI ATCGAT 1 cut(s) 720
BtgI CCRYGG 1 cut(s) 2371
BtsI GCAGTG 1 cut(s) 255
BtsIMutI CAGTG 4 cut(s) 217, 255, 777, 1862
BveI ACCTGC 2 cut(s) 1185, 2888
Cac8I GCNNGC 8 cut(s) 46, 154, 2654, 2785, 2814, 2868, 2895, 3027
CciI TCATGA 4 cut(s) 1128, 1498, 1747, 2644
CfoI GCGC 2 cut(s) 574, 2939
Cfr13I GGNCC 8 cut(s) 493, 668, 740, 1244, 1964, 1965, 2869, 3088
ClaI ATCGAT 1 cut(s) 720
CsiI ACCWGGT 1 cut(s) 630
Csp6I GTAC 7 cut(s) 306, 995, 1142, 1933, 2222, 2290, 2666
CviQI GTAC 7 cut(s) 306, 995, 1142, 1933, 2222, 2290, 2666
DrdI GACNNNNNNGTC 1 cut(s) 1337
DseDI GACNNNNNNGTC 1 cut(s) 1337
Eam1104I CTCTTC 1 cut(s) 141
EarI CTCTTC 1 cut(s) 141
Ecl136II GAGCTC 1 cut(s) 712
Eco130I CCWWGG 3 cut(s) 2001, 2134, 2371
Eco24I GRGCYC 3 cut(s) 714, 1968, 2119
Eco31I GGTCTC 1 cut(s) 1898
Eco47I GGWCC 5 cut(s) 493, 668, 740, 1244, 3088
Eco53kI GAGCTC 1 cut(s) 712
Eco57I CTGAAG 4 cut(s) 144, 807, 1347, 2030
Eco72I CACGTG 1 cut(s) 1012
Eco81I CCTNAGG 1 cut(s) 901
Eco88I CYCGRG 1 cut(s) 2110
Eco91I GGTNACC 2 cut(s) 545, 1172
EcoICRI GAGCTC 1 cut(s) 712
EcoO109I RGGNCCY 4 cut(s) 493, 740, 1964, 3088
EcoO65I GGTNACC 2 cut(s) 545, 1172
EcoRII CCWGG 3 cut(s) 630, 1921, 3084
EcoT14I CCWWGG 3 cut(s) 2001, 2134, 2371
EcoT22I ATGCAT 4 cut(s) 1489, 2629, 2643, 3109
EcoT38I GRGCYC 3 cut(s) 714, 1968, 2119
ErhI CCWWGG 3 cut(s) 2001, 2134, 2371
Esp3I CGTCTC 2 cut(s) 1298, 2399
FaqI GGGAC 6 cut(s) 654, 726, 1422, 2395, 2749, 2761
FauI CCCGC 1 cut(s) 1435
FauNDI CATATG 1 cut(s) 2791
FblI GTMKAC 1 cut(s) 1341
Fnu4HI GCNGC 1 cut(s) 311
FriOI GRGCYC 3 cut(s) 714, 1968, 2119
Fsp4HI GCNGC 1 cut(s) 311
GlaI GCGC 2 cut(s) 573, 2938
GluI GCNGC 1 cut(s) 311
GsaI CCCAGC 1 cut(s) 977
GsuI CTGGAG 4 cut(s) 663, 975, 1166, 2608
HaeIII GGCC 4 cut(s) 1966, 2330, 2870, 3064
HapII CCGG 1 cut(s) 893
HhaI GCGC 2 cut(s) 574, 2939
Hin1I GRCGYC 2 cut(s) 2408, 2774
Hin6I GCGC 2 cut(s) 572, 2937
HinP1I GCGC 2 cut(s) 572, 2937
HincII GTYRAC 4 cut(s) 271, 355, 1137, 1939
HindII GTYRAC 4 cut(s) 271, 355, 1137, 1939
HindIII AAGCTT 3 cut(s) 1258, 1852, 2744
HinfI GANTC 9 cut(s) 1015, 1123, 1287, 1537, 1676, 1744, 1753, 1867, 2282
HpaII CCGG 1 cut(s) 893
Hpy166II GTNNAC 9 cut(s) 192, 271, 355, 1137, 1342, 1418, 1659, 1780, 1939
Hpy8I GTNNAC 9 cut(s) 192, 271, 355, 1137, 1342, 1418, 1659, 1780, 1939
HpyAV CCTTC 7 cut(s) 587, 704, 831, 1442, 2009, 2821, 2984
HpyCH4III ACNGT 3 cut(s) 781, 1141, 1599
HpyCH4IV ACGT 5 cut(s) 90, 1011, 1935, 2408, 2774
HpyF10VI GCNNNNNNNGC 4 cut(s) 107, 2197, 3026, 3070
HpySE526I ACGT 5 cut(s) 90, 1011, 1935, 2408, 2774
Hsp92I GRCGYC 2 cut(s) 2408, 2774
HspAI GCGC 2 cut(s) 572, 2937
LguI GCTCTTC 1 cut(s) 141
LmnI GCTCC 3 cut(s) 956, 978, 1106
Lsp1109I GCAGC 1 cut(s) 297
LweI GCATC 6 cut(s) 905, 1496, 1984, 2185, 2389, 2650
MabI ACCWGGT 1 cut(s) 630
MaeII ACGT 5 cut(s) 90, 1011, 1935, 2408, 2774
MfeI CAATTG 1 cut(s) 3030
MflI RGATCY 1 cut(s) 2309
MhlI GDGCHC 4 cut(s) 714, 1968, 2119, 2443
MlyI GAGTC 4 cut(s) 1281, 1685, 1753, 2276
MmeI TCCRAC 3 cut(s) 1061, 1131, 1925
Mph1103I ATGCAT 4 cut(s) 1489, 2629, 2643, 3109
MroXI GAANNNNTTC 1 cut(s) 1561
MslI CAYNNNNRTG 1 cut(s) 782
MspCI CTTAAG 1 cut(s) 551
MspI CCGG 1 cut(s) 893
MspR9I CCNGG 4 cut(s) 632, 893, 1923, 3086
MunI CAATTG 1 cut(s) 3030
Mva1269I GAATGC 1 cut(s) 3041
MvaI CCWGG 3 cut(s) 632, 1923, 3086
MwoI GCNNNNNNNGC 4 cut(s) 107, 2197, 3026, 3070
NciI CCSGG 1 cut(s) 893
NcoI CCATGG 1 cut(s) 2371
NdeI CATATG 1 cut(s) 2791
NheI GCTAGC 1 cut(s) 3025
NlaIV GGNNCC 8 cut(s) 670, 742, 958, 1030, 1102, 1304, 1965, 1966
NmeAIII GCCGAG 2 cut(s) 154, 1220
NmuCI GTSAC 5 cut(s) 545, 905, 1082, 2501, 2985
NsiI ATGCAT 4 cut(s) 1489, 2629, 2643, 3109
NspI RCATGY 1 cut(s) 3107
NspV TTCGAA 1 cut(s) 1435
PacI TTAATTAA 1 cut(s) 3154
PagI TCATGA 4 cut(s) 1128, 1498, 1747, 2644
PaqCI CACCTGC 1 cut(s) 2888
PciSI GCTCTTC 1 cut(s) 141
PctI GAATGC 1 cut(s) 3041
PdmI GAANNNNTTC 1 cut(s) 1561
PfeI GAWTC 5 cut(s) 1015, 1123, 1537, 1753, 1867
PflFI GACNNNGTC 2 cut(s) 1290, 1413
PflMI CCANNNNNTGG 2 cut(s) 470, 758
PkrI GCNGC 1 cut(s) 312
PleI GAGTC 4 cut(s) 1281, 1684, 1752, 2276
PmaCI CACGTG 1 cut(s) 1012
PmlI CACGTG 1 cut(s) 1012
PpsI GAGTC 4 cut(s) 1281, 1684, 1752, 2276
Ppu21I YACGTR 1 cut(s) 1012
PpuMI RGGWCCY 3 cut(s) 493, 740, 3088
PshBI ATTAAT 1 cut(s) 3150
PsiI TTATAA 2 cut(s) 18, 438
Psp124BI GAGCTC 1 cut(s) 714
Psp1406I AACGTT 1 cut(s) 90
Psp5II RGGWCCY 3 cut(s) 493, 740, 3088
Psp6I CCWGG 3 cut(s) 630, 1921, 3084
PspCI CACGTG 1 cut(s) 1012
PspEI GGTNACC 2 cut(s) 545, 1172
PspFI CCCAGC 1 cut(s) 973
PspGI CCWGG 3 cut(s) 630, 1921, 3084
PspN4I GGNNCC 8 cut(s) 670, 742, 958, 1030, 1102, 1304, 1965, 1966
PspOMI GGGCCC 1 cut(s) 1964
PspPI GGNCC 8 cut(s) 493, 668, 740, 1244, 1964, 1965, 2869, 3088
PspPPI RGGWCCY 3 cut(s) 493, 740, 3088
PsuI RGATCY 1 cut(s) 2309
PsyI GACNNNGTC 2 cut(s) 1290, 1413
RsaI GTAC 7 cut(s) 307, 996, 1143, 1934, 2223, 2291, 2667
RsaNI GTAC 7 cut(s) 306, 995, 1142, 1933, 2222, 2290, 2666
RseI CAYNNNNRTG 1 cut(s) 782
SacI GAGCTC 1 cut(s) 714
SapI GCTCTTC 1 cut(s) 141
SatI GCNGC 1 cut(s) 311
Sau96I GGNCC 8 cut(s) 493, 668, 740, 1244, 1964, 1965, 2869, 3088
SchI GAGTC 4 cut(s) 1281, 1685, 1753, 2276
ScrFI CCNGG 4 cut(s) 632, 893, 1923, 3086
SduI GDGCHC 4 cut(s) 714, 1968, 2119, 2443
SexAI ACCWGGT 1 cut(s) 630
SfaNI GCATC 6 cut(s) 905, 1496, 1984, 2185, 2389, 2650
SfuI TTCGAA 1 cut(s) 1435
SinI GGWCC 5 cut(s) 493, 668, 740, 1244, 3088
SmiMI CAYNNNNRTG 1 cut(s) 782
SmlI CTYRAG 6 cut(s) 488, 551, 1054, 1814, 2554, 2991
SmoI CTYRAG 6 cut(s) 488, 551, 1054, 1814, 2554, 2991
SsiI CCGC 2 cut(s) 1442, 2402
SspI AATATT 2 cut(s) 375, 1189
SstI GAGCTC 1 cut(s) 714
StyD4I CCNGG 4 cut(s) 630, 891, 1921, 3084
StyI CCWWGG 3 cut(s) 2001, 2134, 2371
TaaI ACNGT 3 cut(s) 781, 1141, 1599
TaiI ACGT 5 cut(s) 93, 1014, 1938, 2411, 2777
TaqI TCGA 9 cut(s) 160, 591, 720, 1296, 1435, 1650, 2077, 2671, 2997
TatI WGTACW 5 cut(s) 994, 1141, 2221, 2289, 2665
TfiI GAWTC 5 cut(s) 1015, 1123, 1537, 1753, 1867
TscAI CASTG 4 cut(s) 217, 262, 784, 1869
TseFI GTSAC 5 cut(s) 545, 905, 1082, 2501, 2985
TseI GCWGC 1 cut(s) 310
Tsp45I GTSAC 5 cut(s) 545, 905, 1082, 2501, 2985
TspGWI ACGGA 5 cut(s) 1070, 1280, 1520, 1746, 2973
TspRI CASTG 4 cut(s) 217, 262, 784, 1869
Tth111I GACNNNGTC 2 cut(s) 1290, 1413
Van91I CCANNNNNTGG 2 cut(s) 470, 758
Vha464I CTTAAG 1 cut(s) 551
VpaK11BI GGWCC 5 cut(s) 493, 668, 740, 1244, 3088
VspI ATTAAT 1 cut(s) 3150
XapI RAATTY 8 cut(s) 30, 317, 754, 763, 1494, 1667, 1723, 2578
XbaI TCTAGA 1 cut(s) 1000
XceI RCATGY 1 cut(s) 3107
XcmI CCANNNNNNNNNTGG 1 cut(s) 231
XmiI GTMKAC 1 cut(s) 1341
XmnI GAANNNNTTC 1 cut(s) 1561
ZraI GACGTC 2 cut(s) 2409, 2775
Zsp2I ATGCAT 4 cut(s) 1489, 2629, 2643, 3109
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.