Rw5G000520

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000073
Physical Location & Seq
Reverse (-)
400919 .. 402232
1314 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G000520.1

Sequence Viewer

Length: 1284 bp
ATGGCTTCATCCTCAAATGGTGGTGGTGATATTCCATTTGAAGATGAGGAAATCATTGAGGCTATGTCCATCAATTTCAAAAATAGCGTCGATTTCGTAGACCTGGAGTATGGGATAAACCTTGTGGGTACCCTAATTGCAGATGAAGAACCGGGTCTAGGTGGGACTAAGGCAGCTATCATGAGTATGTGGAAGACACTTGGTCAAGTCAGAATCATCAGGGTTAAGAAGAATCTTGCAACCAAGTTAATCGATGACAGTCCATGGAACGTCAAGGGTTATTGTTTCACGGTACGTCACTGGCCACCTCGCCATGCTATTGATGATCTTGAGCCACACAGAGCTACCTATTGGATACAAGCACATGGTATTCCAATGGACCAGATATCTACTCATAATGGAAGAAAGCTGGGCAACCTCTTGGGCTCAGTTATTGATGTGGAAGATCCTCGAGTCTATGGGAACAGAGGATTTATGAGGATTAGAGTGGATTTTGATACCAGAAGACCTCTAGCTACCTTCTGCCAGCTTCCTAGAAATAACTCTGCTACGACAAAGATCAGACTACGTTATGAGAACCTGAAATGCTTCTGCTTCAAGTGCGGGAGGCTAGGCCATATGGTGACTGCGTGCAGATACCAGGTAAACCCAATTCTGTTGAAATTTGGGGTTGTGTATGATAACTCTCTGGTTGCTGAACCTCTACAGAAACCAGTGTTCACACAAGCACACTTTCCACCGGATTTCCCTTATGCTCCGATAACCGGAATATTCCGAAGGAGCAGGAAGGAGATTCCAAACAAATTCAAATATATGGGTGTCAGACATGATGTGACAGAGGATACCTGTGAGGGAGAAGGGTCCAAATCTAAACTCTGCTCAAGGCTTGAAAATGATGGCTCTTCCTCACCAATTATGTCAGATGGTCAACTTTCTGTGTCAACAAAGGGGATACAAGGAAAAAGAAACACTAAGCAGTGGGTAGACATGTGGGATCCTGACACACATGGCTCACTATACAGGAACGGGAGTGTTACCGTTGCAATGAATGGGCTGAGCATCAAGCCTAACTCCTGGGCTGATCCCGAGTCCATCCCTCCTTGGGCCTTCAAGAATAAGGAGGACTTCCTTAGAGCTAACCCAATGTTTCCTATTCCTGGCTACATTGAGGTTGAGCTGGAGCCCACTATCACCCCACCCAACTTTACCTCACCAAAAATTGTTGAGTTACAGCAATCTGCCCCCCCCCTCTGGACTCATCCACTTTTCGAAAAAGGAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 RVT_3 zf-CCHC_4

Protein Analysis

427

Amino Acids

48.15

Weight (kDa)

8.49

Isoelectric Point (pI)

32.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CCHC_4 PF14392 165 - 213 1.1e-08 Zinc knuckle
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000073)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g17821 FvH4_2g04610 FvH4_2g09611 FvH4_2g13563 FvH4_3g21493 FvH4_3g32115 FvH4_3g35060 FvH4_4g00220 FvH4_4g06410 FvH4_4g10367 FvH4_4g10367 FvH4_4g10367 FvH4_5g06101 FvH4_5g08842 FvH4_5g27366 FvH4_5g31283 FvH4_6g11611 FvH4_6g23032 FvH4_7g05661 FvH4_7g06172
malus_domestica MD13G1263700.v1.1
prunus_persica Prupe.1G292300_v2.0.a1 Prupe.5G009700_v2.0.a1 Prupe.6G158100_v2.0.a1 Prupe.8G025100_v2.0.a1 Prupe.8G042600_v2.0.a1
pyrus_communis pycom02g02250 pycom02g16030 pycom05g10490 pycom09g06450 pycom09g06460 pycom13g26200 pycom16g17700
rosa_chinensis RchiOBHm_Chr2g0163321 RchiOBHm_Chr4g0401981 RchiOBHm_Chr5g0005991 RchiOBHm_Chr5g0027191 RchiOBHm_Chr6g0249771
rosa_laevigata RLG00000001040 RLG00000001764 RLG00000002239 RLG00000002240 RLG00000003259 RLG00000008993 RLG00000013847 RLG00000014178 RLG00000015576 RLG00000017269 RLG00000017355 RLG00000018127 RLG00000018602 RLG00000018615 RLG00000019904 RLG00000020044 RLG00000021849 RLG00000021850 RLG00000027965 RLG00000029266 RLG00000029820 RLG00000031112 RLG00000031915 RLG00000033568 RLG00000035013 RLG00000035677 RLG00000036568 RLG00000036628
rosa_multiflora Rmu_co8254085.1_g000001 Rmu_co8462383.1_g000001 Rmu_sc0000185.1_g000049 Rmu_sc0000251.1_g000018 Rmu_sc0000367.1_g000094 Rmu_sc0000498.1_g000023 Rmu_sc0000868.1_g000005 Rmu_sc0001521.1_g000047 Rmu_sc0001534.1_g000007 Rmu_sc0002014.1_g000012 Rmu_sc0003214.1_g000009 Rmu_sc0003573.1_g000041 Rmu_sc0003832.1_g000023 Rmu_sc0004677.1_g000015 Rmu_sc0004967.1_g000014 Rmu_sc0005426.1_g000001 Rmu_sc0005621.1_g000004 Rmu_sc0005640.1_g000009 Rmu_sc0005947.1_g000034 Rmu_sc0006889.1_g000011 Rmu_sc0007121.1_g000014 Rmu_sc0009191.1_g000017 Rmu_sc0010556.1_g000011 Rmu_sc0011396.1_g000007 Rmu_sc0019862.1_g000002 Rmu_sc0022931.1_g000003 Rmu_sc0024164.1_g000001 Rmu_ssc0000391.1_g000027
rosa_roxburghii Rroxscaffold_1G00050060 Rroxscaffold_1G00061810 Rroxscaffold_2G00109590 Rroxscaffold_2G00113810 Rroxscaffold_2G00124040 Rroxscaffold_2G00143620 Rroxscaffold_2G00154760 Rroxscaffold_4G00302820 Rroxscaffold_4G00302830 Rroxscaffold_4G00308680 Rroxscaffold_4G00310240 Rroxscaffold_4G00327890 Rroxscaffold_5G00334600 Rroxscaffold_5G00336050 Rroxscaffold_5G00346560 Rroxscaffold_5G00347860 Rroxscaffold_5G00347870 Rroxscaffold_5G00349330 Rroxscaffold_5G00351980 Rroxscaffold_6G00395280 Rroxscaffold_6G00396750 Rroxscaffold_6G00402910 Rroxscaffold_7G00174790 Rroxscaffold_7G00177570 Rroxscaffold_7G00191820 Rroxscaffold_7G00203480 Rroxscaffold_7G00204380
rosa_rugosa Rorug01G0106600 Rorug01G0106600 Rorug01G0148500.1 Rorug02G0224200 Rorug02G0225500 Rorug02G0233000 Rorug02G0630400 Rorug03G0198000 Rorug03G0232600.1 Rorug03G0240500 Rorug04G0032900 Rorug04G0073300 Rorug04G0118500 Rorug04G0149600.1 Rorug04G0205100 Rorug04G0256700 Rorug05G0020200 Rorug05G0050000 Rorug05G0247300 Rorug05G0295100 Rorug05G0337900 Rorug05G0542600 Rorug06G0034800 Rorug06G0035100 Rorug06G0220100 Rorug07G0091900 Rorug07G0271200
rosa_samantha Rh1AG118200 Rh1AG253000 Rh1AG296300 Rh1AG409000 Rh1AG410600 Rh1BG073400 Rh1BG130400 Rh1BG147300 Rh1BG163600 Rh1CG016000 Rh1DG012200 Rh1DG012300 Rh1DG078600 Rh1DG078800 Rh1DG138100 Rh1DG179200 Rh1DG192600 Rh1DG194500 Rh1DG194600 Rh1DG214300 Rh1DG245900 Rh1DG267000 Rh1DG322700 Rh1DG442700 Rh2AG282000 Rh2AG423000 Rh2AG423100 Rh2AG460700 Rh2AG466200 Rh2AG513400 Rh2AG547000 Rh2BG277800 Rh2BG281200 Rh2BG298300 Rh2BG304300 Rh2BG433500 Rh2CG276000 Rh2CG282600 Rh2CG325500 Rh2CG393000 Rh2DG319200 Rh2DG443400 Rh3AG169300 Rh3AG303600 Rh3BG195000 Rh3BG282600 Rh3BG343400 Rh3BG343500 Rh3BG344100 Rh3CG111700 Rh3CG185700 Rh3CG259100 Rh3CG293000 Rh3CG317900 Rh3CG340900 Rh3CG341000 Rh3CG341100 Rh3CG354700 Rh3CG359200 Rh3CG368100 Rh3DG179900 Rh3DG288700 Rh3DG302600 Rh3DG316000 Rh3DG324500 Rh3DG324600 Rh3DG343200 Rh3DG343300 Rh3DG362400 Rh4AG071800 Rh4BG034000 Rh4BG044300 Rh4BG044400 Rh4BG069600 Rh4BG157400 Rh4CG007000 Rh4CG030400 Rh4CG037900 Rh4CG077700 Rh4CG115000 Rh4CG162400 Rh4CG162500 Rh4CG165500 Rh4CG165600 Rh4CG172900 Rh4CG239700 Rh4DG055900 Rh4DG173200 Rh5AG354900 Rh5AG511100 Rh5BG112600 Rh5BG541000 Rh5BG541100 Rh5BG549300 Rh5CG004400 Rh5CG315000 Rh5CG563800 Rh5CG572100 Rh5DG112200 Rh5DG191300 Rh5DG407600 Rh5DG407700 Rh5DG567500 Rh6AG002600 Rh6AG051400 Rh6AG199800 Rh6AG199900 Rh6AG206200 Rh6AG391000 Rh6AG391100 Rh6BG007500 Rh6BG053400 Rh6BG225900 Rh6BG227100 Rh6BG232400 Rh6CG091100 Rh6CG125400 Rh6CG186700 Rh6CG222700 Rh6CG222800 Rh6CG235500 Rh6CG247200 Rh6CG331200 Rh6CG465100 Rh6DG183500 Rh6DG213500 Rh6DG238800 Rh6DG263700 Rh7AG211800 Rh7AG441800 Rh7BG008600 Rh7BG253000 Rh7BG390000 Rh7CG189600 Rh7CG401500 Rh7CG424200 Rh7CG428700 Rh7CG479800 Rh7CG494300 Rh7DG177000 Rh7DG218700 Rh7DG254000 Rh7DG294800 Rh7DG407200 Rh7DG448800 Rh7DG461500
rosa_wichuraiana Rw0G012180 Rw1G005270 Rw1G012310 Rw1G016890 Rw1G021700 Rw2G014380 Rw2G027690 Rw3G016400 Rw3G028230 Rw3G029320 Rw4G002060 Rw4G002100 Rw4G003980 Rw4G014140 Rw4G014770 Rw4G014780 Rw4G015760 Rw4G021230 Rw5G000520 Rw5G035610 Rw6G001680 Rw6G017890 Rw6G027440 Rw6G034130 Rw6G034170 Rw7G012430 Rw7G033870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 128
AccB1I GGYRCC 1 cut(s) 128
AccI GTMKAC 2 cut(s) 99, 984
AciI CCGC 1 cut(s) 603
AclWI GGATC 4 cut(s) 440, 989, 1002, 1076
AcoI YGGCCR 1 cut(s) 302
AcsI RAATTY 2 cut(s) 662, 803
AfaI GTAC 2 cut(s) 130, 294
AfiI CCNNNNNNNGG 5 cut(s) 158, 764, 1102, 1157, 1251
AflIII ACRYGT 1 cut(s) 987
AgsI TTSAA 7 cut(s) 41, 79, 598, 661, 808, 890, 1111
AhdI GACNNNNNGTC 1 cut(s) 201
AjnI CCWGG 4 cut(s) 102, 639, 1073, 1156
AluBI AGCT 7 cut(s) 176, 344, 409, 515, 529, 1136, 1177
AluI AGCT 7 cut(s) 176, 344, 409, 515, 529, 1136, 1177
AlwI GGATC 4 cut(s) 440, 989, 1002, 1076
Ama87I CYCGRG 2 cut(s) 450, 1085
AoxI GGCC 3 cut(s) 302, 613, 1104
ApeKI GCWGC 1 cut(s) 173
ApoI RAATTY 2 cut(s) 662, 803
ArsI GACNNNNNNTTYG 2 cut(s) 72, 104
Asp700I GAANNNNTTC 1 cut(s) 587
Asp718I GGTACC 1 cut(s) 128
AspS9I GGNCC 3 cut(s) 379, 861, 1104
AsuC2I CCSGG 1 cut(s) 153
AsuHPI GGTGA 5 cut(s) 38, 634, 900, 1183, 1203
AsuII TTCGAA 1 cut(s) 1269
AvaI CYCGRG 2 cut(s) 450, 1085
AvaII GGWCC 2 cut(s) 379, 861
BalI TGGCCA 1 cut(s) 304
BamHI GGATCC 1 cut(s) 994
BanI GGYRCC 1 cut(s) 128
BanII GRGCYC 2 cut(s) 428, 1185
BbsI GAAGAC 2 cut(s) 200, 511
BbvI GCAGC 1 cut(s) 185
BccI CCATC 4 cut(s) 77, 890, 917, 1100
BciT130I CCWGG 4 cut(s) 104, 641, 1075, 1158
BciVI GTATCC 3 cut(s) 348, 835, 945
BcnI CCSGG 1 cut(s) 153
BfaI CTAG 4 cut(s) 158, 512, 534, 611
BfmI CTRYAG 1 cut(s) 704
BfuI GTATCC 3 cut(s) 348, 835, 945
BisI GCNGC 1 cut(s) 174
BlpI GCTNAGC 1 cut(s) 1055
BlsI GCNGC 1 cut(s) 175
Bme1390I CCNGG 5 cut(s) 104, 153, 641, 1075, 1158
Bme18I GGWCC 2 cut(s) 379, 861
BmeRI GACNNNNNGTC 1 cut(s) 201
BmeT110I CYCGRG 2 cut(s) 450, 1085
BmgT120I GGNCC 3 cut(s) 379, 861, 1104
BmiI GGNNCC 4 cut(s) 130, 862, 996, 1182
BmrFI CCNGG 5 cut(s) 104, 153, 641, 1075, 1158
BmsI GCATC 1 cut(s) 1068
BpiI GAAGAC 2 cut(s) 200, 511
BpmI CTGGAG 2 cut(s) 125, 1199
Bpu1102I GCTNAGC 1 cut(s) 1055
Bpu14I TTCGAA 1 cut(s) 1269
BpuEI CTTGAG 2 cut(s) 350, 865
BpuMI CCSGG 1 cut(s) 153
Bsa29I ATCGAT 1 cut(s) 252
BsaJI CCNNGG 3 cut(s) 263, 1074, 1100
BsaWI WCCGGW 2 cut(s) 739, 764
Bsc4I CCNNNNNNNGG 5 cut(s) 158, 764, 1102, 1157, 1251
Bse1I ACTGG 2 cut(s) 305, 713
Bse3DI GCAATG 1 cut(s) 1050
BseBI CCWGG 4 cut(s) 104, 641, 1075, 1158
BseCI ATCGAT 1 cut(s) 252
BseDI CCNNGG 3 cut(s) 263, 1074, 1100
BseGI GGATG 3 cut(s) 8, 1092, 1258
BseLI CCNNNNNNNGG 5 cut(s) 158, 764, 1102, 1157, 1251
BseMI GCAATG 1 cut(s) 1050
BseMII CTCAG 2 cut(s) 441, 1046
BseNI ACTGG 2 cut(s) 305, 713
BseXI GCAGC 1 cut(s) 185
BseYI CCCAGC 1 cut(s) 409
BsgI GTGCAG 1 cut(s) 652
BshFI GGCC 3 cut(s) 304, 615, 1106
BshNI GGYRCC 1 cut(s) 128
BshVI ATCGAT 1 cut(s) 252
BsiHKCI CYCGRG 2 cut(s) 450, 1085
BsiSI CCGG 3 cut(s) 152, 740, 765
BslFI GGGAC 1 cut(s) 178
BslI CCNNNNNNNGG 5 cut(s) 158, 764, 1102, 1157, 1251
BsmFI GGGAC 1 cut(s) 178
BsnI GGCC 3 cut(s) 304, 615, 1106
BsoBI CYCGRG 2 cut(s) 450, 1085
Bsp119I TTCGAA 1 cut(s) 1269
Bsp1286I GDGCHC 2 cut(s) 428, 1185
Bsp143I GATC 5 cut(s) 325, 445, 558, 994, 1081
Bsp1720I GCTNAGC 1 cut(s) 1055
Bsp19I CCATGG 1 cut(s) 263
BspACI CCGC 1 cut(s) 603
BspANI GGCC 3 cut(s) 304, 615, 1106
BspCNI CTCAG 2 cut(s) 440, 1047
BspDI ATCGAT 1 cut(s) 252
BspHI TCATGA 1 cut(s) 180
BspLI GGNNCC 4 cut(s) 130, 862, 996, 1182
BspPI GGATC 4 cut(s) 440, 989, 1002, 1076
BspQI GCTCTTC 1 cut(s) 907
BspT104I TTCGAA 1 cut(s) 1269
BspT107I GGYRCC 1 cut(s) 128
BsrDI GCAATG 1 cut(s) 1050
BsrI ACTGG 2 cut(s) 305, 713
BssECI CCNNGG 3 cut(s) 263, 1074, 1100
BssMI GATC 5 cut(s) 325, 445, 558, 994, 1081
BssT1I CCWWGG 2 cut(s) 263, 1100
Bst2UI CCWGG 4 cut(s) 104, 641, 1075, 1158
Bst4CI ACNGT 3 cut(s) 260, 292, 1039
Bst6I CTCTTC 1 cut(s) 907
BstBI TTCGAA 1 cut(s) 1269
BstC8I GCNNGC 2 cut(s) 527, 631
BstDEI CTNAG 5 cut(s) 168, 427, 972, 1055, 1130
BstDSI CCRYGG 1 cut(s) 263
BstF5I GGATG 3 cut(s) 8, 1092, 1258
BstKTI GATC 5 cut(s) 328, 448, 561, 997, 1084
BstMBI GATC 5 cut(s) 325, 445, 558, 994, 1081
BstMWI GCNNNNNNNGC 1 cut(s) 600
BstNI CCWGG 4 cut(s) 104, 641, 1075, 1158
BstNSI RCATGY 1 cut(s) 991
BstSCI CCNGG 5 cut(s) 102, 151, 639, 1073, 1156
BstSFI CTRYAG 1 cut(s) 704
BstV1I GCAGC 1 cut(s) 185
BstV2I GAAGAC 2 cut(s) 200, 511
BstX2I RGATCY 2 cut(s) 445, 994
BstYI RGATCY 2 cut(s) 445, 994
Bsu15I ATCGAT 1 cut(s) 252
BsuI GTATCC 3 cut(s) 348, 835, 945
BsuRI GGCC 3 cut(s) 304, 615, 1106
BsuTUI ATCGAT 1 cut(s) 252
BtgI CCRYGG 1 cut(s) 263
BtsCI GGATG 3 cut(s) 8, 1092, 1258
BtsI GCAGTG 1 cut(s) 983
BtsIMutI CAGTG 3 cut(s) 298, 720, 983
Cac8I GCNNGC 2 cut(s) 527, 631
CciI TCATGA 1 cut(s) 180
Cfr13I GGNCC 3 cut(s) 379, 861, 1104
ClaI ATCGAT 1 cut(s) 252
CseI GACGC 1 cut(s) 76
CsiI ACCWGGT 1 cut(s) 639
Csp6I GTAC 2 cut(s) 129, 293
CviAII CATG 7 cut(s) 181, 264, 314, 365, 827, 988, 1007
CviQI GTAC 2 cut(s) 129, 293
DdeI CTNAG 5 cut(s) 168, 427, 972, 1055, 1130
DpnI GATC 5 cut(s) 327, 447, 560, 996, 1083
DpnII GATC 5 cut(s) 325, 445, 558, 994, 1081
DriI GACNNNNNGTC 1 cut(s) 201
EaeI YGGCCR 1 cut(s) 302
Eam1104I CTCTTC 1 cut(s) 907
Eam1105I GACNNNNNGTC 1 cut(s) 201
EarI CTCTTC 1 cut(s) 907
Eco130I CCWWGG 2 cut(s) 263, 1100
Eco24I GRGCYC 2 cut(s) 428, 1185
Eco32I GATATC 1 cut(s) 387
Eco47I GGWCC 2 cut(s) 379, 861
Eco88I CYCGRG 2 cut(s) 450, 1085
EcoRII CCWGG 4 cut(s) 102, 639, 1073, 1156
EcoRV GATATC 1 cut(s) 387
EcoT14I CCWWGG 2 cut(s) 263, 1100
EcoT38I GRGCYC 2 cut(s) 428, 1185
ErhI CCWWGG 2 cut(s) 263, 1100
FaeI CATG 7 cut(s) 184, 267, 317, 368, 830, 991, 1010
FalI AAGNNNNNCTT 2 cut(s) 1109, 1141
FaqI GGGAC 1 cut(s) 178
FatI CATG 7 cut(s) 180, 263, 313, 364, 826, 987, 1006
FauI CCCGC 1 cut(s) 596
FauNDI CATATG 1 cut(s) 618
FblI GTMKAC 2 cut(s) 99, 984
Fnu4HI GCNGC 1 cut(s) 174
FokI GGATG 2 cut(s) 1079, 1245
FriOI GRGCYC 2 cut(s) 428, 1185
Fsp4HI GCNGC 1 cut(s) 174
FspBI CTAG 4 cut(s) 158, 512, 534, 611
GluI GCNGC 1 cut(s) 174
GsaI CCCAGC 1 cut(s) 413
GsuI CTGGAG 2 cut(s) 125, 1199
HaeIII GGCC 3 cut(s) 304, 615, 1106
HapII CCGG 3 cut(s) 152, 740, 765
HgaI GACGC 1 cut(s) 76
Hin1II CATG 7 cut(s) 184, 267, 317, 368, 830, 991, 1010
HincII GTYRAC 2 cut(s) 929, 942
HindII GTYRAC 2 cut(s) 929, 942
HinfI GANTC 6 cut(s) 213, 232, 453, 793, 1088, 1255
HpaII CCGG 3 cut(s) 152, 740, 765
HphI GGTGA 5 cut(s) 38, 634, 900, 1183, 1203
Hpy166II GTNNAC 6 cut(s) 100, 646, 720, 929, 942, 985
Hpy188I TCNGA 6 cut(s) 212, 563, 759, 776, 824, 922
Hpy188III TCNNGA 6 cut(s) 181, 329, 998, 1085, 1111, 1252
Hpy8I GTNNAC 6 cut(s) 100, 646, 720, 929, 942, 985
Hpy99I CGWCG 1 cut(s) 92
HpyAV CCTTC 5 cut(s) 529, 771, 781, 851, 1117
HpyCH4III ACNGT 3 cut(s) 260, 292, 1039
HpyCH4IV ACGT 3 cut(s) 270, 295, 568
HpyCH4V TGCA 4 cut(s) 140, 239, 633, 1043
HpyF10VI GCNNNNNNNGC 1 cut(s) 600
HpyF3I CTNAG 5 cut(s) 168, 427, 972, 1055, 1130
HpySE526I ACGT 3 cut(s) 270, 295, 568
Hsp92II CATG 7 cut(s) 184, 267, 317, 368, 830, 991, 1010
KpnI GGTACC 1 cut(s) 132
Kzo9I GATC 5 cut(s) 325, 445, 558, 994, 1081
LguI GCTCTTC 1 cut(s) 907
LmnI GCTCC 3 cut(s) 760, 780, 1180
Lsp1109I GCAGC 1 cut(s) 185
LweI GCATC 1 cut(s) 1068
MabI ACCWGGT 1 cut(s) 639
MaeI CTAG 4 cut(s) 158, 512, 534, 611
MaeII ACGT 3 cut(s) 270, 295, 568
MaeIII GTNAC 5 cut(s) 296, 622, 832, 1033, 1227
MalI GATC 5 cut(s) 327, 447, 560, 996, 1083
MboI GATC 5 cut(s) 325, 445, 558, 994, 1081
MboII GAAGA 8 cut(s) 53, 158, 205, 241, 414, 455, 516, 894
MflI RGATCY 2 cut(s) 445, 994
MhlI GDGCHC 2 cut(s) 428, 1185
MlsI TGGCCA 1 cut(s) 304
MluCI AATT 7 cut(s) 73, 135, 651, 662, 803, 912, 1218
MluNI TGGCCA 1 cut(s) 304
MlyI GAGTC 3 cut(s) 462, 1097, 1249
Mox20I TGGCCA 1 cut(s) 304
MroXI GAANNNNTTC 1 cut(s) 587
MscI TGGCCA 1 cut(s) 304
MseI TTAA 2 cut(s) 225, 248
MslI CAYNNNNRTG 1 cut(s) 185
Msp20I TGGCCA 1 cut(s) 304
MspI CCGG 3 cut(s) 152, 740, 765
MspR9I CCNGG 5 cut(s) 104, 153, 641, 1075, 1158
MvaI CCWGG 4 cut(s) 104, 641, 1075, 1158
MwoI GCNNNNNNNGC 1 cut(s) 600
NciI CCSGG 1 cut(s) 153
NcoI CCATGG 1 cut(s) 263
NdeI CATATG 1 cut(s) 618
NdeII GATC 5 cut(s) 325, 445, 558, 994, 1081
NlaIII CATG 7 cut(s) 184, 267, 317, 368, 830, 991, 1010
NlaIV GGNNCC 4 cut(s) 130, 862, 996, 1182
NmuCI GTSAC 3 cut(s) 296, 622, 832
NspI RCATGY 1 cut(s) 991
NspV TTCGAA 1 cut(s) 1269
PaeR7I CTCGAG 1 cut(s) 450
PagI TCATGA 1 cut(s) 180
PciI ACATGT 1 cut(s) 987
PciSI GCTCTTC 1 cut(s) 907
PdmI GAANNNNTTC 1 cut(s) 587
PfeI GAWTC 3 cut(s) 213, 232, 793
PkrI GCNGC 1 cut(s) 175
PleI GAGTC 3 cut(s) 461, 1096, 1249
PpsI GAGTC 3 cut(s) 461, 1096, 1249
PscI ACATGT 1 cut(s) 987
Psp6I CCWGG 4 cut(s) 102, 639, 1073, 1156
PspFI CCCAGC 1 cut(s) 409
PspGI CCWGG 4 cut(s) 102, 639, 1073, 1156
PspN4I GGNNCC 4 cut(s) 130, 862, 996, 1182
PspPI GGNCC 3 cut(s) 379, 861, 1104
PspXI VCTCGAGB 1 cut(s) 450
PsuI RGATCY 2 cut(s) 445, 994
RsaI GTAC 2 cut(s) 130, 294
RsaNI GTAC 2 cut(s) 129, 293
RseI CAYNNNNRTG 1 cut(s) 185
SapI GCTCTTC 1 cut(s) 907
SaqAI TTAA 2 cut(s) 225, 248
SatI GCNGC 1 cut(s) 174
Sau3AI GATC 5 cut(s) 325, 445, 558, 994, 1081
Sau96I GGNCC 3 cut(s) 379, 861, 1104
SchI GAGTC 3 cut(s) 462, 1097, 1249
ScrFI CCNGG 5 cut(s) 104, 153, 641, 1075, 1158
SduI GDGCHC 2 cut(s) 428, 1185
SexAI ACCWGGT 1 cut(s) 639
SfaNI GCATC 1 cut(s) 1068
SfcI CTRYAG 1 cut(s) 704
Sfr274I CTCGAG 1 cut(s) 450
SfuI TTCGAA 1 cut(s) 1269
SinI GGWCC 2 cut(s) 379, 861
SlaI CTCGAG 1 cut(s) 450
SmiMI CAYNNNNRTG 1 cut(s) 185
SmlI CTYRAG 3 cut(s) 329, 450, 880
SmoI CTYRAG 3 cut(s) 329, 450, 880
Sse9I AATT 7 cut(s) 73, 135, 651, 662, 803, 912, 1218
SsiI CCGC 1 cut(s) 603
SspI AATATT 1 cut(s) 771
SspMI CTAG 4 cut(s) 158, 512, 534, 611
StyD4I CCNGG 5 cut(s) 102, 151, 639, 1073, 1156
StyI CCWWGG 2 cut(s) 263, 1100
TaaI ACNGT 3 cut(s) 260, 292, 1039
TaiI ACGT 3 cut(s) 273, 298, 571
TaqI TCGA 4 cut(s) 90, 252, 451, 1269
TasI AATT 7 cut(s) 73, 135, 651, 662, 803, 912, 1218
TfiI GAWTC 3 cut(s) 213, 232, 793
Tru1I TTAA 2 cut(s) 225, 248
Tru9I TTAA 2 cut(s) 225, 248
TscAI CASTG 3 cut(s) 305, 720, 983
TseFI GTSAC 3 cut(s) 296, 622, 832
TseI GCWGC 1 cut(s) 173
Tsp45I GTSAC 3 cut(s) 296, 622, 832
TspDTI ATGAA 2 cut(s) 159, 1061
TspRI CASTG 3 cut(s) 305, 720, 983
VpaK11BI GGWCC 2 cut(s) 379, 861
XapI RAATTY 2 cut(s) 662, 803
XceI RCATGY 1 cut(s) 991
XhoI CTCGAG 1 cut(s) 450
XmiI GTMKAC 2 cut(s) 99, 984
XmnI GAANNNNTTC 1 cut(s) 587
XspI CTAG 4 cut(s) 158, 512, 534, 611
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.