Rh2DG443400

Domain of unknown function (DUF4283)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0000073
Physical Location & Seq
Reverse (-)
64396774 .. 64398062
1289 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG443400.1

Sequence Viewer

Length: 846 bp
ATGGCTGAAGATAGTAGCAATACTGATAATCTTATTATTCAAATGAGAGATAGGCTCTCAATTGGGTCTTCCAACCAAAAGATTCGCCTGGCTGCTGTGATCTTTGCAGACAAAGCTCCCCACAAGCCATCCACAATTGATATGATCAAAACTGCATGGGTAGGCTTTGGAGGTATCAAAATCACAGCCAGGGATCTTTGGAACCCCGCTGACCACCTTTTTGCGATCACTGCCGATGATGAAAATGTTGCCAAGCTGATCCTAGACAACTCCCCATGGACCATCAAAGGCTTCTCCCTTCATGTCCAGGATCCACTTGAAGGGGAGGAGGGTACTTTCCCCTTCCTTAGAGTTCGTATGCTAATTGATGCTAGGAAACCCATACCTACTGGTTTCAAGCTTCCAAGAGAGAATGGGTCTGTTTCTTGGGTGGAATTCCGACTTGGTCATGTGGACTCTTCTAGATTCTCATGCCCCAATGGTCGTGAACCAGGGTACGAAGAAGAAGAATATGGAGCTTTCATGGCTATTGACGCCCTTCGCCGTCCAGTTATTCCTCCAAACACCCCTCAGTCGGAATCCCGGCCTGCCCGTCGGAGATCTGCTGGACAAGTCATTGGTCCACCGGGTTTTGAAACTACTGGACAAAGGCCCACTTCTCAATGCAACTTGAGCAACAAGAGTCACATGACCGTTGAGAAATGCAGTGTGGAGTGGCACAAACCCCACAATCCTGACAGAGTCATCACCGCATCAACTTTGCCGTGTTCAACTAAAGATGCCTATGGGTGCTACACTACCCCATCACATGGCAATGCAGTTCCATGCATACCCATCCATGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 zf-CCHC_4 zf-RING_2

Protein Analysis

281

Amino Acids

31.06

Weight (kDa)

7.11

Isoelectric Point (pI)

40.72

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000073)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g17821 FvH4_2g04610 FvH4_2g09611 FvH4_2g13563 FvH4_3g21493 FvH4_3g32115 FvH4_3g35060 FvH4_4g00220 FvH4_4g06410 FvH4_4g10367 FvH4_4g10367 FvH4_4g10367 FvH4_5g06101 FvH4_5g08842 FvH4_5g27366 FvH4_5g31283 FvH4_6g11611 FvH4_6g23032 FvH4_7g05661 FvH4_7g06172
malus_domestica MD13G1263700.v1.1
prunus_persica Prupe.1G292300_v2.0.a1 Prupe.5G009700_v2.0.a1 Prupe.6G158100_v2.0.a1 Prupe.8G025100_v2.0.a1 Prupe.8G042600_v2.0.a1
pyrus_communis pycom02g02250 pycom02g16030 pycom05g10490 pycom09g06450 pycom09g06460 pycom13g26200 pycom16g17700
rosa_chinensis RchiOBHm_Chr2g0163321 RchiOBHm_Chr4g0401981 RchiOBHm_Chr5g0005991 RchiOBHm_Chr5g0027191 RchiOBHm_Chr6g0249771
rosa_laevigata RLG00000001040 RLG00000001764 RLG00000002239 RLG00000002240 RLG00000003259 RLG00000008993 RLG00000013847 RLG00000014178 RLG00000015576 RLG00000017269 RLG00000017355 RLG00000018127 RLG00000018602 RLG00000018615 RLG00000019904 RLG00000020044 RLG00000021849 RLG00000021850 RLG00000027965 RLG00000029266 RLG00000029820 RLG00000031112 RLG00000031915 RLG00000033568 RLG00000035013 RLG00000035677 RLG00000036568 RLG00000036628
rosa_multiflora Rmu_co8254085.1_g000001 Rmu_co8462383.1_g000001 Rmu_sc0000185.1_g000049 Rmu_sc0000251.1_g000018 Rmu_sc0000367.1_g000094 Rmu_sc0000498.1_g000023 Rmu_sc0000868.1_g000005 Rmu_sc0001521.1_g000047 Rmu_sc0001534.1_g000007 Rmu_sc0002014.1_g000012 Rmu_sc0003214.1_g000009 Rmu_sc0003573.1_g000041 Rmu_sc0003832.1_g000023 Rmu_sc0004677.1_g000015 Rmu_sc0004967.1_g000014 Rmu_sc0005426.1_g000001 Rmu_sc0005621.1_g000004 Rmu_sc0005640.1_g000009 Rmu_sc0005947.1_g000034 Rmu_sc0006889.1_g000011 Rmu_sc0007121.1_g000014 Rmu_sc0009191.1_g000017 Rmu_sc0010556.1_g000011 Rmu_sc0011396.1_g000007 Rmu_sc0019862.1_g000002 Rmu_sc0022931.1_g000003 Rmu_sc0024164.1_g000001 Rmu_ssc0000391.1_g000027
rosa_roxburghii Rroxscaffold_1G00050060 Rroxscaffold_1G00061810 Rroxscaffold_2G00109590 Rroxscaffold_2G00113810 Rroxscaffold_2G00124040 Rroxscaffold_2G00143620 Rroxscaffold_2G00154760 Rroxscaffold_4G00302820 Rroxscaffold_4G00302830 Rroxscaffold_4G00308680 Rroxscaffold_4G00310240 Rroxscaffold_4G00327890 Rroxscaffold_5G00334600 Rroxscaffold_5G00336050 Rroxscaffold_5G00346560 Rroxscaffold_5G00347860 Rroxscaffold_5G00347870 Rroxscaffold_5G00349330 Rroxscaffold_5G00351980 Rroxscaffold_6G00395280 Rroxscaffold_6G00396750 Rroxscaffold_6G00402910 Rroxscaffold_7G00174790 Rroxscaffold_7G00177570 Rroxscaffold_7G00191820 Rroxscaffold_7G00203480 Rroxscaffold_7G00204380
rosa_rugosa Rorug01G0106600 Rorug01G0106600 Rorug01G0148500.1 Rorug02G0224200 Rorug02G0225500 Rorug02G0233000 Rorug02G0630400 Rorug03G0198000 Rorug03G0232600.1 Rorug03G0240500 Rorug04G0032900 Rorug04G0073300 Rorug04G0118500 Rorug04G0149600.1 Rorug04G0205100 Rorug04G0256700 Rorug05G0020200 Rorug05G0050000 Rorug05G0247300 Rorug05G0295100 Rorug05G0337900 Rorug05G0542600 Rorug06G0034800 Rorug06G0035100 Rorug06G0220100 Rorug07G0091900 Rorug07G0271200
rosa_samantha Rh1AG118200 Rh1AG253000 Rh1AG296300 Rh1AG409000 Rh1AG410600 Rh1BG073400 Rh1BG130400 Rh1BG147300 Rh1BG163600 Rh1CG016000 Rh1DG012200 Rh1DG012300 Rh1DG078600 Rh1DG078800 Rh1DG138100 Rh1DG179200 Rh1DG192600 Rh1DG194500 Rh1DG194600 Rh1DG214300 Rh1DG245900 Rh1DG267000 Rh1DG322700 Rh1DG442700 Rh2AG282000 Rh2AG423000 Rh2AG423100 Rh2AG460700 Rh2AG466200 Rh2AG513400 Rh2AG547000 Rh2BG277800 Rh2BG281200 Rh2BG298300 Rh2BG304300 Rh2BG433500 Rh2CG276000 Rh2CG282600 Rh2CG325500 Rh2CG393000 Rh2DG319200 Rh2DG443400 Rh3AG169300 Rh3AG303600 Rh3BG195000 Rh3BG282600 Rh3BG343400 Rh3BG343500 Rh3BG344100 Rh3CG111700 Rh3CG185700 Rh3CG259100 Rh3CG293000 Rh3CG317900 Rh3CG340900 Rh3CG341000 Rh3CG341100 Rh3CG354700 Rh3CG359200 Rh3CG368100 Rh3DG179900 Rh3DG288700 Rh3DG302600 Rh3DG316000 Rh3DG324500 Rh3DG324600 Rh3DG343200 Rh3DG343300 Rh3DG362400 Rh4AG071800 Rh4BG034000 Rh4BG044300 Rh4BG044400 Rh4BG069600 Rh4BG157400 Rh4CG007000 Rh4CG030400 Rh4CG037900 Rh4CG077700 Rh4CG115000 Rh4CG162400 Rh4CG162500 Rh4CG165500 Rh4CG165600 Rh4CG172900 Rh4CG239700 Rh4DG055900 Rh4DG173200 Rh5AG354900 Rh5AG511100 Rh5BG112600 Rh5BG541000 Rh5BG541100 Rh5BG549300 Rh5CG004400 Rh5CG315000 Rh5CG563800 Rh5CG572100 Rh5DG112200 Rh5DG191300 Rh5DG407600 Rh5DG407700 Rh5DG567500 Rh6AG002600 Rh6AG051400 Rh6AG199800 Rh6AG199900 Rh6AG206200 Rh6AG391000 Rh6AG391100 Rh6BG007500 Rh6BG053400 Rh6BG225900 Rh6BG227100 Rh6BG232400 Rh6CG091100 Rh6CG125400 Rh6CG186700 Rh6CG222700 Rh6CG222800 Rh6CG235500 Rh6CG247200 Rh6CG331200 Rh6CG465100 Rh6DG183500 Rh6DG213500 Rh6DG238800 Rh6DG263700 Rh7AG211800 Rh7AG441800 Rh7BG008600 Rh7BG253000 Rh7BG390000 Rh7CG189600 Rh7CG401500 Rh7CG424200 Rh7CG428700 Rh7CG479800 Rh7CG494300 Rh7DG177000 Rh7DG218700 Rh7DG254000 Rh7DG294800 Rh7DG407200 Rh7DG448800 Rh7DG461500
rosa_wichuraiana Rw0G012180 Rw1G005270 Rw1G012310 Rw1G016890 Rw1G021700 Rw2G014380 Rw2G027690 Rw3G016400 Rw3G028230 Rw3G029320 Rw4G002060 Rw4G002100 Rw4G003980 Rw4G014140 Rw4G014770 Rw4G014780 Rw4G015760 Rw4G021230 Rw5G000520 Rw5G035610 Rw6G001680 Rw6G017890 Rw6G027440 Rw6G034130 Rw6G034170 Rw7G012430 Rw7G033870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 809
AciI CCGC 2 cut(s) 207, 750
AclWI GGATC 4 cut(s) 201, 253, 305, 318
AcsI RAATTY 1 cut(s) 434
AcuI CTGAAG 1 cut(s) 27
AcyI GRCGYC 1 cut(s) 534
AfaI GTAC 2 cut(s) 334, 497
AfiI CCNNNNNNNGG 4 cut(s) 320, 574, 809, 839
AgsI TTSAA 5 cut(s) 41, 320, 397, 635, 771
AjnI CCWGG 4 cut(s) 87, 188, 306, 490
AjuI GAANNNNNNNTTGG 2 cut(s) 426, 458
AluBI AGCT 4 cut(s) 116, 256, 400, 518
AluI AGCT 4 cut(s) 116, 256, 400, 518
AlwI GGATC 4 cut(s) 201, 253, 305, 318
AoxI GGCC 2 cut(s) 584, 650
ApeKI GCWGC 1 cut(s) 92
ApoI RAATTY 1 cut(s) 434
ArsI GACNNNNNNTTYG 2 cut(s) 203, 235
AspS9I GGNCC 3 cut(s) 279, 620, 651
AsuC2I CCSGG 2 cut(s) 583, 627
AsuHPI GGTGA 1 cut(s) 739
AvaII GGWCC 2 cut(s) 279, 620
BamHI GGATCC 1 cut(s) 310
BbsI GAAGAC 1 cut(s) 60
BbvI GCAGC 1 cut(s) 79
BccI CCATC 4 cut(s) 136, 290, 811, 842
BceAI ACGGC 2 cut(s) 528, 748
BciT130I CCWGG 4 cut(s) 89, 190, 308, 492
BclI TGATCA 1 cut(s) 144
BcnI CCSGG 2 cut(s) 583, 627
BfaI CTAG 3 cut(s) 263, 372, 462
BglII AGATCT 1 cut(s) 599
BisI GCNGC 1 cut(s) 93
BlsI GCNGC 1 cut(s) 94
Bme1390I CCNGG 6 cut(s) 89, 190, 308, 492, 583, 627
Bme18I GGWCC 2 cut(s) 279, 620
BmgT120I GGNCC 3 cut(s) 279, 620, 651
BmiI GGNNCC 2 cut(s) 203, 312
BmrFI CCNGG 6 cut(s) 89, 190, 308, 492, 583, 627
BmsI GCATC 3 cut(s) 358, 761, 769
BpiI GAAGAC 1 cut(s) 60
BplI GAGNNNNNCTC 2 cut(s) 39, 71
BpuEI CTTGAG 1 cut(s) 691
BpuMI CCSGG 2 cut(s) 583, 627
BsaHI GRCGYC 1 cut(s) 534
BsaJI CCNNGG 4 cut(s) 189, 275, 491, 838
Bsc4I CCNNNNNNNGG 4 cut(s) 320, 574, 809, 839
Bse1I ACTGG 3 cut(s) 394, 548, 646
Bse3DI GCAATG 1 cut(s) 820
BseBI CCWGG 4 cut(s) 89, 190, 308, 492
BseDI CCNNGG 4 cut(s) 189, 275, 491, 838
BseGI GGATG 2 cut(s) 128, 834
BseLI CCNNNNNNNGG 4 cut(s) 320, 574, 809, 839
BseMI GCAATG 1 cut(s) 820
BseMII CTCAG 1 cut(s) 584
BseNI ACTGG 3 cut(s) 394, 548, 646
BseRI GAGGAG 1 cut(s) 341
BseXI GCAGC 1 cut(s) 79
BshFI GGCC 2 cut(s) 586, 652
BsiSI CCGG 2 cut(s) 583, 626
BslI CCNNNNNNNGG 4 cut(s) 320, 574, 809, 839
BsnI GGCC 2 cut(s) 586, 652
Bsp143I GATC 7 cut(s) 99, 144, 193, 225, 258, 310, 599
Bsp19I CCATGG 2 cut(s) 275, 838
BspACI CCGC 2 cut(s) 207, 750
BspANI GGCC 2 cut(s) 586, 652
BspCNI CTCAG 1 cut(s) 583
BspLI GGNNCC 2 cut(s) 203, 312
BspPI GGATC 4 cut(s) 201, 253, 305, 318
BsrDI GCAATG 1 cut(s) 820
BsrI ACTGG 3 cut(s) 394, 548, 646
BssECI CCNNGG 4 cut(s) 189, 275, 491, 838
BssMI GATC 7 cut(s) 99, 144, 193, 225, 258, 310, 599
BssNI GRCGYC 1 cut(s) 534
BssT1I CCWWGG 2 cut(s) 275, 838
Bst2UI CCWGG 4 cut(s) 89, 190, 308, 492
Bst4CI ACNGT 1 cut(s) 694
Bst6I CTCTTC 1 cut(s) 463
BstACI GRCGYC 1 cut(s) 534
BstC8I GCNNGC 1 cut(s) 588
BstDEI CTNAG 2 cut(s) 347, 570
BstDSI CCRYGG 2 cut(s) 275, 838
BstF5I GGATG 2 cut(s) 128, 834
BstKTI GATC 7 cut(s) 102, 147, 196, 228, 261, 313, 602
BstMBI GATC 7 cut(s) 99, 144, 193, 225, 258, 310, 599
BstMWI GCNNNNNNNGC 5 cut(s) 113, 230, 524, 533, 672
BstNI CCWGG 4 cut(s) 89, 190, 308, 492
BstSCI CCNGG 6 cut(s) 87, 188, 306, 490, 581, 625
BstV1I GCAGC 1 cut(s) 79
BstV2I GAAGAC 1 cut(s) 60
BstX2I RGATCY 3 cut(s) 193, 310, 599
BstYI RGATCY 3 cut(s) 193, 310, 599
BsuRI GGCC 2 cut(s) 586, 652
BtgI CCRYGG 2 cut(s) 275, 838
BtsCI GGATG 2 cut(s) 128, 834
BtsI GCAGTG 2 cut(s) 228, 712
BtsIMutI CAGTG 2 cut(s) 228, 712
Cac8I GCNNGC 1 cut(s) 588
Cfr13I GGNCC 3 cut(s) 279, 620, 651
CseI GACGC 1 cut(s) 542
Csp6I GTAC 2 cut(s) 333, 496
CviQI GTAC 2 cut(s) 333, 496
DdeI CTNAG 2 cut(s) 347, 570
DpnI GATC 7 cut(s) 101, 146, 195, 227, 260, 312, 601
DpnII GATC 7 cut(s) 99, 144, 193, 225, 258, 310, 599
Eam1104I CTCTTC 1 cut(s) 463
EarI CTCTTC 1 cut(s) 463
Eco130I CCWWGG 2 cut(s) 275, 838
Eco47I GGWCC 2 cut(s) 279, 620
Eco57I CTGAAG 1 cut(s) 27
EcoRI GAATTC 1 cut(s) 434
EcoRII CCWGG 4 cut(s) 87, 188, 306, 490
EcoT14I CCWWGG 2 cut(s) 275, 838
EcoT22I ATGCAT 1 cut(s) 830
ErhI CCWWGG 2 cut(s) 275, 838
FalI AAGNNNNNCTT 2 cut(s) 640, 672
FauI CCCGC 1 cut(s) 214
FbaI TGATCA 1 cut(s) 144
Fnu4HI GCNGC 1 cut(s) 93
FokI GGATG 2 cut(s) 115, 821
Fsp4HI GCNGC 1 cut(s) 93
FspBI CTAG 3 cut(s) 263, 372, 462
GluI GCNGC 1 cut(s) 93
HaeIII GGCC 2 cut(s) 586, 652
HapII CCGG 2 cut(s) 583, 626
HgaI GACGC 1 cut(s) 542
Hin1I GRCGYC 1 cut(s) 534
HindIII AAGCTT 1 cut(s) 398
HinfI GANTC 6 cut(s) 82, 455, 465, 578, 682, 741
HpaII CCGG 2 cut(s) 583, 626
HphI GGTGA 1 cut(s) 739
Hpy166II GTNNAC 3 cut(s) 454, 488, 623
Hpy188I TCNGA 3 cut(s) 440, 577, 597
Hpy188III TCNNGA 3 cut(s) 462, 485, 734
Hpy8I GTNNAC 3 cut(s) 454, 488, 623
Hpy99I CGWCG 1 cut(s) 597
HpyAV CCTTC 4 cut(s) 308, 314, 352, 548
HpyCH4III ACNGT 1 cut(s) 694
HpyCH4V TGCA 6 cut(s) 107, 155, 666, 705, 818, 828
HpyF10VI GCNNNNNNNGC 5 cut(s) 113, 230, 524, 533, 672
HpyF3I CTNAG 2 cut(s) 347, 570
Hsp92I GRCGYC 1 cut(s) 534
Ksp22I TGATCA 1 cut(s) 144
Kzo9I GATC 7 cut(s) 99, 144, 193, 225, 258, 310, 599
LmnI GCTCC 2 cut(s) 121, 515
Lsp1109I GCAGC 1 cut(s) 79
LweI GCATC 3 cut(s) 358, 761, 769
MaeI CTAG 3 cut(s) 263, 372, 462
MaeIII GTNAC 1 cut(s) 683
MalI GATC 7 cut(s) 101, 146, 195, 227, 260, 312, 601
MboI GATC 7 cut(s) 99, 144, 193, 225, 258, 310, 599
MboII GAAGA 6 cut(s) 20, 60, 450, 512, 515, 518
MfeI CAATTG 2 cut(s) 60, 135
MflI RGATCY 3 cut(s) 193, 310, 599
MluCI AATT 4 cut(s) 60, 135, 363, 434
MlyI GAGTC 3 cut(s) 449, 691, 750
MmeI TCCRAC 4 cut(s) 96, 463, 555, 575
MnlI CCTC 5 cut(s) 164, 319, 322, 567, 579
Mph1103I ATGCAT 1 cut(s) 830
MslI CAYNNNNRTG 1 cut(s) 813
MspA1I CMGCKG 1 cut(s) 209
MspI CCGG 2 cut(s) 583, 626
MspR9I CCNGG 6 cut(s) 89, 190, 308, 492, 583, 627
MunI CAATTG 2 cut(s) 60, 135
MvaI CCWGG 4 cut(s) 89, 190, 308, 492
MwoI GCNNNNNNNGC 5 cut(s) 113, 230, 524, 533, 672
NciI CCSGG 2 cut(s) 583, 627
NcoI CCATGG 2 cut(s) 275, 838
NdeII GATC 7 cut(s) 99, 144, 193, 225, 258, 310, 599
NlaIV GGNNCC 2 cut(s) 203, 312
NmuCI GTSAC 1 cut(s) 683
NsiI ATGCAT 1 cut(s) 830
PfeI GAWTC 3 cut(s) 82, 465, 578
PflFI GACNNNGTC 2 cut(s) 444, 740
PflMI CCANNNNNTGG 1 cut(s) 809
PfoI TCCNGGA 1 cut(s) 306
PkrI GCNGC 1 cut(s) 94
PleI GAGTC 3 cut(s) 449, 690, 749
PpsI GAGTC 3 cut(s) 449, 690, 749
Psp6I CCWGG 4 cut(s) 87, 188, 306, 490
PspGI CCWGG 4 cut(s) 87, 188, 306, 490
PspN4I GGNNCC 2 cut(s) 203, 312
PspPI GGNCC 3 cut(s) 279, 620, 651
PsuI RGATCY 3 cut(s) 193, 310, 599
PsyI GACNNNGTC 2 cut(s) 444, 740
RsaI GTAC 2 cut(s) 334, 497
RsaNI GTAC 2 cut(s) 333, 496
RseI CAYNNNNRTG 1 cut(s) 813
SatI GCNGC 1 cut(s) 93
Sau3AI GATC 7 cut(s) 99, 144, 193, 225, 258, 310, 599
Sau96I GGNCC 3 cut(s) 279, 620, 651
SchI GAGTC 3 cut(s) 449, 691, 750
ScrFI CCNGG 6 cut(s) 89, 190, 308, 492, 583, 627
SetI ASST 7 cut(s) 118, 175, 219, 258, 388, 402, 520
SfaNI GCATC 3 cut(s) 358, 761, 769
SinI GGWCC 2 cut(s) 279, 620
SmiMI CAYNNNNRTG 1 cut(s) 813
SmlI CTYRAG 1 cut(s) 670
SmoI CTYRAG 1 cut(s) 670
Sse9I AATT 4 cut(s) 60, 135, 363, 434
SsiI CCGC 2 cut(s) 207, 750
SspMI CTAG 3 cut(s) 263, 372, 462
StyD4I CCNGG 6 cut(s) 87, 188, 306, 490, 581, 625
StyI CCWWGG 2 cut(s) 275, 838
TaaI ACNGT 1 cut(s) 694
TasI AATT 4 cut(s) 60, 135, 363, 434
TfiI GAWTC 3 cut(s) 82, 465, 578
TscAI CASTG 2 cut(s) 235, 712
TseFI GTSAC 1 cut(s) 683
TseI GCWGC 1 cut(s) 92
Tsp45I GTSAC 1 cut(s) 683
TspDTI ATGAA 3 cut(s) 255, 290, 511
TspRI CASTG 2 cut(s) 235, 712
Tth111I GACNNNGTC 2 cut(s) 444, 740
Van91I CCANNNNNTGG 1 cut(s) 809
VpaK11BI GGWCC 2 cut(s) 279, 620
XapI RAATTY 1 cut(s) 434
XbaI TCTAGA 1 cut(s) 461
XspI CTAG 3 cut(s) 263, 372, 462
Zsp2I ATGCAT 1 cut(s) 830
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.