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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 1 cut(s) | 203 |
| AciI | CCGC | 3 cut(s) | 251, 271, 342 |
| AclI | AACGTT | 1 cut(s) | 510 |
| AclWI | GGATC | 6 cut(s) | 49, 62, 332, 345, 387, 400 |
| AcoI | YGGCCR | 1 cut(s) | 154 |
| AfaI | GTAC | 1 cut(s) | 286 |
| AfeI | AGCGCT | 1 cut(s) | 469 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 328, 329, 610, 611 |
| AgsI | TTSAA | 3 cut(s) | 65, 236, 245 |
| AjnI | CCWGG | 3 cut(s) | 93, 461, 594 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 508, 540 |
| AluBI | AGCT | 5 cut(s) | 68, 160, 388, 409, 417 |
| AluI | AGCT | 5 cut(s) | 68, 160, 388, 409, 417 |
| Alw26I | GTCTC | 1 cut(s) | 472 |
| AlwI | GGATC | 6 cut(s) | 49, 62, 332, 345, 387, 400 |
| Aor51HI | AGCGCT | 1 cut(s) | 469 |
| AoxI | GGCC | 1 cut(s) | 154 |
| AseI | ATTAAT | 1 cut(s) | 494 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 510 |
| AspLEI | GCGC | 1 cut(s) | 470 |
| AsuHPI | GGTGA | 4 cut(s) | 25, 83, 106, 346 |
| BalI | TGGCCA | 1 cut(s) | 156 |
| BamHI | GGATCC | 3 cut(s) | 54, 337, 392 |
| BanII | GRGCYC | 1 cut(s) | 461 |
| BbsI | GAAGAC | 1 cut(s) | 252 |
| BccI | CCATC | 5 cut(s) | 137, 261, 317, 443, 574 |
| BceAI | ACGGC | 1 cut(s) | 386 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 131, 165 |
| BciT130I | CCWGG | 3 cut(s) | 95, 463, 596 |
| BcoDI | GTCTC | 1 cut(s) | 472 |
| BfaI | CTAG | 4 cut(s) | 137, 288, 410, 482 |
| BfmI | CTRYAG | 1 cut(s) | 404 |
| BfoI | RGCGCY | 1 cut(s) | 471 |
| Bme1390I | CCNGG | 3 cut(s) | 95, 463, 596 |
| BmiI | GGNNCC | 6 cut(s) | 56, 99, 339, 394, 460, 555 |
| BmrFI | CCNGG | 3 cut(s) | 95, 463, 596 |
| BmsI | GCATC | 1 cut(s) | 372 |
| BpiI | GAAGAC | 1 cut(s) | 252 |
| BpmI | CTGGAG | 2 cut(s) | 282, 445 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 328, 329, 610, 611 |
| Bse1I | ACTGG | 1 cut(s) | 265 |
| Bse3DI | GCAATG | 1 cut(s) | 345 |
| BseBI | CCWGG | 3 cut(s) | 95, 463, 596 |
| BseGI | GGATG | 4 cut(s) | 272, 309, 363, 591 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 328, 329, 610, 611 |
| BseMI | GCAATG | 1 cut(s) | 345 |
| BseNI | ACTGG | 1 cut(s) | 265 |
| BseRI | GAGGAG | 1 cut(s) | 152 |
| BseYI | CCCAGC | 2 cut(s) | 4, 84 |
| Bsh1236I | CGCG | 1 cut(s) | 203 |
| BshFI | GGCC | 1 cut(s) | 156 |
| BslFI | GGGAC | 2 cut(s) | 241, 518 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 328, 329, 610, 611 |
| BsmAI | GTCTC | 1 cut(s) | 472 |
| BsmFI | GGGAC | 2 cut(s) | 241, 518 |
| BsnI | GGCC | 1 cut(s) | 156 |
| Bsp1286I | GDGCHC | 1 cut(s) | 461 |
| Bsp143I | GATC | 5 cut(s) | 15, 22, 54, 337, 392 |
| BspACI | CCGC | 3 cut(s) | 251, 271, 342 |
| BspANI | GGCC | 1 cut(s) | 156 |
| BspFNI | CGCG | 1 cut(s) | 203 |
| BspLI | GGNNCC | 6 cut(s) | 56, 99, 339, 394, 460, 555 |
| BspPI | GGATC | 6 cut(s) | 49, 62, 332, 345, 387, 400 |
| BsrDI | GCAATG | 1 cut(s) | 345 |
| BsrI | ACTGG | 1 cut(s) | 265 |
| BssMI | GATC | 5 cut(s) | 15, 22, 54, 337, 392 |
| Bst2UI | CCWGG | 3 cut(s) | 95, 463, 596 |
| Bst4CI | ACNGT | 2 cut(s) | 46, 232 |
| Bst6I | CTCTTC | 1 cut(s) | 43 |
| BstC8I | GCNNGC | 3 cut(s) | 154, 158, 601 |
| BstF5I | GGATG | 4 cut(s) | 272, 309, 363, 591 |
| BstFNI | CGCG | 1 cut(s) | 203 |
| BstH2I | RGCGCY | 1 cut(s) | 471 |
| BstHHI | GCGC | 1 cut(s) | 470 |
| BstKTI | GATC | 5 cut(s) | 18, 25, 57, 340, 395 |
| BstMAI | GTCTC | 1 cut(s) | 472 |
| BstMBI | GATC | 5 cut(s) | 15, 22, 54, 337, 392 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 465 |
| BstNI | CCWGG | 3 cut(s) | 95, 463, 596 |
| BstSCI | CCNGG | 3 cut(s) | 93, 461, 594 |
| BstSFI | CTRYAG | 1 cut(s) | 404 |
| BstUI | CGCG | 1 cut(s) | 203 |
| BstV2I | GAAGAC | 1 cut(s) | 252 |
| BstX2I | RGATCY | 3 cut(s) | 54, 337, 392 |
| BstYI | RGATCY | 3 cut(s) | 54, 337, 392 |
| BsuRI | GGCC | 1 cut(s) | 156 |
| BtsCI | GGATG | 4 cut(s) | 272, 309, 363, 591 |
| BtsIMutI | CAGTG | 1 cut(s) | 258 |
| Cac8I | GCNNGC | 3 cut(s) | 154, 158, 601 |
| CfoI | GCGC | 1 cut(s) | 470 |
| Csp6I | GTAC | 1 cut(s) | 285 |
| CviAII | CATG | 3 cut(s) | 10, 180, 334 |
| CviQI | GTAC | 1 cut(s) | 285 |
| DpnI | GATC | 5 cut(s) | 17, 24, 56, 339, 394 |
| DpnII | GATC | 5 cut(s) | 15, 22, 54, 337, 392 |
| EaeI | YGGCCR | 1 cut(s) | 154 |
| Eam1104I | CTCTTC | 1 cut(s) | 43 |
| EarI | CTCTTC | 1 cut(s) | 43 |
| Eco24I | GRGCYC | 1 cut(s) | 461 |
| Eco32I | GATATC | 1 cut(s) | 589 |
| Eco47III | AGCGCT | 1 cut(s) | 469 |
| EcoRII | CCWGG | 3 cut(s) | 93, 461, 594 |
| EcoRV | GATATC | 1 cut(s) | 589 |
| EcoT38I | GRGCYC | 1 cut(s) | 461 |
| FaeI | CATG | 3 cut(s) | 13, 183, 337 |
| FaiI | YATR | 7 cut(s) | 11, 181, 255, 335, 383, 551, 575 |
| FaqI | GGGAC | 2 cut(s) | 241, 518 |
| FatI | CATG | 3 cut(s) | 9, 179, 333 |
| FauI | CCCGC | 3 cut(s) | 258, 264, 349 |
| FokI | GGATG | 4 cut(s) | 279, 296, 350, 578 |
| FriOI | GRGCYC | 1 cut(s) | 461 |
| FspBI | CTAG | 4 cut(s) | 137, 288, 410, 482 |
| GlaI | GCGC | 1 cut(s) | 469 |
| GsaI | CCCAGC | 2 cut(s) | 8, 88 |
| GsuI | CTGGAG | 2 cut(s) | 282, 445 |
| HaeII | RGCGCY | 1 cut(s) | 471 |
| HaeIII | GGCC | 1 cut(s) | 156 |
| HhaI | GCGC | 1 cut(s) | 470 |
| Hin1II | CATG | 3 cut(s) | 13, 183, 337 |
| Hin6I | GCGC | 1 cut(s) | 468 |
| HinP1I | GCGC | 1 cut(s) | 468 |
| HindIII | AAGCTT | 2 cut(s) | 66, 415 |
| HinfI | GANTC | 6 cut(s) | 61, 143, 222, 276, 291, 302 |
| HphI | GGTGA | 4 cut(s) | 25, 83, 106, 346 |
| Hpy166II | GTNNAC | 4 cut(s) | 91, 124, 199, 501 |
| Hpy188I | TCNGA | 4 cut(s) | 22, 215, 368, 610 |
| Hpy188III | TCNNGA | 4 cut(s) | 58, 137, 226, 295 |
| Hpy8I | GTNNAC | 4 cut(s) | 91, 124, 199, 501 |
| Hpy99I | CGWCG | 2 cut(s) | 46, 372 |
| HpyAV | CCTTC | 2 cut(s) | 290, 615 |
| HpyCH4III | ACNGT | 2 cut(s) | 46, 232 |
| HpyCH4IV | ACGT | 2 cut(s) | 111, 510 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 465 |
| HpySE526I | ACGT | 2 cut(s) | 111, 510 |
| Hsp92II | CATG | 3 cut(s) | 13, 183, 337 |
| HspAI | GCGC | 1 cut(s) | 468 |
| Kzo9I | GATC | 5 cut(s) | 15, 22, 54, 337, 392 |
| LmnI | GCTCC | 5 cut(s) | 165, 385, 464, 465, 559 |
| LweI | GCATC | 1 cut(s) | 372 |
| MaeI | CTAG | 4 cut(s) | 137, 288, 410, 482 |
| MaeII | ACGT | 2 cut(s) | 111, 510 |
| MaeIII | GTNAC | 1 cut(s) | 112 |
| MalI | GATC | 5 cut(s) | 17, 24, 56, 339, 394 |
| MboI | GATC | 5 cut(s) | 15, 22, 54, 337, 392 |
| MboII | GAAGA | 3 cut(s) | 30, 257, 458 |
| MflI | RGATCY | 3 cut(s) | 54, 337, 392 |
| MhlI | GDGCHC | 1 cut(s) | 461 |
| MlsI | TGGCCA | 1 cut(s) | 156 |
| MluCI | AATT | 2 cut(s) | 315, 491 |
| MluNI | TGGCCA | 1 cut(s) | 156 |
| MlyI | GAGTC | 4 cut(s) | 137, 231, 296, 300 |
| MmeI | TCCRAC | 2 cut(s) | 391, 539 |
| MnlI | CCTC | 6 cut(s) | 39, 111, 173, 198, 257, 604 |
| Mox20I | TGGCCA | 1 cut(s) | 156 |
| MroXI | GAANNNNTTC | 1 cut(s) | 510 |
| MscI | TGGCCA | 1 cut(s) | 156 |
| MseI | TTAA | 2 cut(s) | 209, 494 |
| Msp20I | TGGCCA | 1 cut(s) | 156 |
| MspR9I | CCNGG | 3 cut(s) | 95, 463, 596 |
| MvaI | CCWGG | 3 cut(s) | 95, 463, 596 |
| MvnI | CGCG | 1 cut(s) | 203 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 465 |
| NdeII | GATC | 5 cut(s) | 15, 22, 54, 337, 392 |
| NlaIII | CATG | 3 cut(s) | 13, 183, 337 |
| NlaIV | GGNNCC | 6 cut(s) | 56, 99, 339, 394, 460, 555 |
| NmuCI | GTSAC | 1 cut(s) | 112 |
| PdmI | GAANNNNTTC | 1 cut(s) | 510 |
| PfeI | GAWTC | 2 cut(s) | 61, 276 |
| PfoI | TCCNGGA | 1 cut(s) | 461 |
| PleI | GAGTC | 4 cut(s) | 137, 230, 296, 299 |
| PpsI | GAGTC | 4 cut(s) | 137, 230, 296, 299 |
| PshBI | ATTAAT | 1 cut(s) | 494 |
| Psp1406I | AACGTT | 1 cut(s) | 510 |
| Psp6I | CCWGG | 3 cut(s) | 93, 461, 594 |
| PspFI | CCCAGC | 2 cut(s) | 4, 84 |
| PspGI | CCWGG | 3 cut(s) | 93, 461, 594 |
| PspN4I | GGNNCC | 6 cut(s) | 56, 99, 339, 394, 460, 555 |
| PsuI | RGATCY | 3 cut(s) | 54, 337, 392 |
| RsaI | GTAC | 1 cut(s) | 286 |
| RsaNI | GTAC | 1 cut(s) | 285 |
| SaqAI | TTAA | 2 cut(s) | 209, 494 |
| Sau3AI | GATC | 5 cut(s) | 15, 22, 54, 337, 392 |
| SchI | GAGTC | 4 cut(s) | 137, 231, 296, 300 |
| ScrFI | CCNGG | 3 cut(s) | 95, 463, 596 |
| SduI | GDGCHC | 1 cut(s) | 461 |
| SfaNI | GCATC | 1 cut(s) | 372 |
| SfcI | CTRYAG | 1 cut(s) | 404 |
| Sse9I | AATT | 2 cut(s) | 315, 491 |
| SsiI | CCGC | 3 cut(s) | 251, 271, 342 |
| SspMI | CTAG | 4 cut(s) | 137, 288, 410, 482 |
| StyD4I | CCNGG | 3 cut(s) | 93, 461, 594 |
| TaaI | ACNGT | 2 cut(s) | 46, 232 |
| TaiI | ACGT | 2 cut(s) | 114, 513 |
| TaqI | TCGA | 1 cut(s) | 41 |
| TasI | AATT | 2 cut(s) | 315, 491 |
| TfiI | GAWTC | 2 cut(s) | 61, 276 |
| Tru1I | TTAA | 2 cut(s) | 209, 494 |
| Tru9I | TTAA | 2 cut(s) | 209, 494 |
| TscAI | CASTG | 1 cut(s) | 265 |
| TseFI | GTSAC | 1 cut(s) | 112 |
| Tsp45I | GTSAC | 1 cut(s) | 112 |
| TspDTI | ATGAA | 1 cut(s) | 168 |
| TspRI | CASTG | 1 cut(s) | 265 |
| VspI | ATTAAT | 1 cut(s) | 494 |
| XbaI | TCTAGA | 1 cut(s) | 136 |
| XmnI | GAANNNNTTC | 1 cut(s) | 510 |
| XspI | CTAG | 4 cut(s) | 137, 288, 410, 482 |