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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 47 |
| AciI | CCGC | 1 cut(s) | 38 |
| AdeI | CACNNNGTG | 1 cut(s) | 63 |
| AoxI | GGCC | 2 cut(s) | 105, 160 |
| AspS9I | GGNCC | 1 cut(s) | 106 |
| BccI | CCATC | 1 cut(s) | 180 |
| BfaI | CTAG | 1 cut(s) | 196 |
| BmgT120I | GGNCC | 1 cut(s) | 106 |
| BmsI | GCATC | 2 cut(s) | 16, 177 |
| BsaBI | GATNNNNATC | 1 cut(s) | 51 |
| Bse8I | GATNNNNATC | 1 cut(s) | 51 |
| BseJI | GATNNNNATC | 1 cut(s) | 51 |
| BseMII | CTCAG | 1 cut(s) | 171 |
| BshFI | GGCC | 2 cut(s) | 107, 162 |
| BsnI | GGCC | 2 cut(s) | 107, 162 |
| BspACI | CCGC | 1 cut(s) | 38 |
| BspANI | GGCC | 2 cut(s) | 107, 162 |
| BspCNI | CTCAG | 1 cut(s) | 170 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 140 |
| BstDEI | CTNAG | 1 cut(s) | 157 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 35, 140 |
| BsuRI | GGCC | 2 cut(s) | 107, 162 |
| Cfr13I | GGNCC | 1 cut(s) | 106 |
| CviAII | CATG | 1 cut(s) | 34 |
| CviJI | RGCY | 2 cut(s) | 107, 162 |
| CviKI_1 | RGCY | 2 cut(s) | 107, 162 |
| DdeI | CTNAG | 1 cut(s) | 157 |
| DraIII | CACNNNGTG | 1 cut(s) | 63 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 47 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 47 |
| EciI | GGCGGA | 1 cut(s) | 53 |
| Eco147I | AGGCCT | 1 cut(s) | 162 |
| FaeI | CATG | 1 cut(s) | 37 |
| FaiI | YATR | 3 cut(s) | 35, 111, 113 |
| FatI | CATG | 1 cut(s) | 33 |
| FspBI | CTAG | 1 cut(s) | 196 |
| HaeIII | GGCC | 2 cut(s) | 107, 162 |
| Hin1II | CATG | 1 cut(s) | 37 |
| HinfI | GANTC | 1 cut(s) | 7 |
| Hpy188III | TCNNGA | 2 cut(s) | 20, 184 |
| Hpy99I | CGWCG | 1 cut(s) | 47 |
| HpyAV | CCTTC | 1 cut(s) | 97 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 35, 140 |
| HpyF3I | CTNAG | 1 cut(s) | 157 |
| Hsp92II | CATG | 1 cut(s) | 37 |
| LpnPI | CCDG | 2 cut(s) | 45, 144 |
| LweI | GCATC | 2 cut(s) | 16, 177 |
| MaeI | CTAG | 1 cut(s) | 196 |
| MboII | GAAGA | 1 cut(s) | 35 |
| MnlI | CCTC | 3 cut(s) | 124, 179, 191 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 35, 140 |
| NlaIII | CATG | 1 cut(s) | 37 |
| PceI | AGGCCT | 1 cut(s) | 162 |
| PfeI | GAWTC | 1 cut(s) | 7 |
| PflFI | GACNNNGTC | 1 cut(s) | 47 |
| PspPI | GGNCC | 1 cut(s) | 106 |
| PsyI | GACNNNGTC | 1 cut(s) | 47 |
| Sau96I | GGNCC | 1 cut(s) | 106 |
| SetI | ASST | 2 cut(s) | 90, 197 |
| SfaNI | GCATC | 2 cut(s) | 16, 177 |
| SgeI | CNNG | 6 cut(s) | 32, 46, 72, 133, 152, 171 |
| SseBI | AGGCCT | 1 cut(s) | 162 |
| SsiI | CCGC | 1 cut(s) | 38 |
| SspMI | CTAG | 1 cut(s) | 196 |
| StuI | AGGCCT | 1 cut(s) | 162 |
| TaqI | TCGA | 1 cut(s) | 84 |
| TfiI | GAWTC | 1 cut(s) | 7 |
| Tth111I | GACNNNGTC | 1 cut(s) | 47 |
| XspI | CTAG | 1 cut(s) | 196 |