FvH4_5g27366

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000073
Physical Location & Seq
Forward (+)
18659904 .. 18663322
3419 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g27366.t1

Sequence Viewer

Length: 2916 bp
ATGGCCACCAACCCAGATACCCGTGAGGAAATTCCTTACGAGGATGACTCTATTATTGAAGCTATGTCTGTAAACTTTGACATGAGTGCAGATCTCCTTGATCTCAGTGACAGTATTGACCTTGTTGGCACACTTATTGCTGATGTTGAGCCAGGTGTAAGTGGTATCAAAGTTGCTCTCATGGGTATATGGCGCTCACTGGGACAGATCTGTATAGTCAGAGTCAAGGTGAATACTTACAATTTGTCGGTGGGTTCTGAGAAACTTGCCCGAAAGCTTCTACCTGAAAGTCCATGGAACGTGAAGGGTTATTGCTTCACAATTCGTCATTGGCCAGGTTCAGTGCTGGAGGTAGAGGATCCCAAATTGGTCGGTAATCGTGGTTTCATCCGTATAAGGGTGGATTTTGATACAAGAAAGCCTTTGGCAACCTTTGTTAATTTCCCTAGACCAAATGGGGTAACAAAGCGCATCCGGCTGCAGTATGAAAATTTGAAGAGCTTTTGTCACAATTGTGGTAGATTGGGGCATATTAGCACCCCATGCAATCTTCAGATGCACCCGACTCTGGTAAGGCTTGGAGTGGAATACAATCAGGCCTTGGTTGCCGAGAGTCTTCAGAAGCATGTGTTCACTCAGTCCACGTTTCCGACTAAATTCCCTTATGTTCTGACGACCGGAATCTTTGTCCAGGGAAACACAAGGGGTAAAGACTCACTCAATTCAAATTCCTCACTCAATTCAAATTCTAGGGATAGGGGTGGATGTGACAAGAGCTTTTCTTCCATGTCTTTGAAACCAACTACTTCAAATCAACTTTCGGCGGTTTGTGTCAGTACTCCGGAGGTTAACATGGGTAAGTCTAGCTGTAGGGGGCTGGATTTGAGCAGTGAAAGTTGGGCTGATTTGGAAAGCTTACCTCCTTGGGCTTTTAGTTCTCAAGAGGCCTACCTGAAAGATAATCCTTGGTTCCCTTGTCTTTCAAGGACAGATACTGATGATATTCCTATGCTCATTAAAAATAGACCAGTAGGGATTCATATTGAAGAGTTGCCAAGAGCTCTTGACTCTTTGGCTCAACCCTATGCTCTTTTGGAGATTGATGCTACTGCCCCCATCAAACGGAAGCATCAGACAAAGAAAAAAGGAGAAGGAGAGGCTTCTAGGATTACAAAGAAACAAAAGGTGATGAGTCTGAGATTAAGGATGTCTTCTTTCGGGCGTAGAGGAAGGGGTAGAGGCAGTAGAAGCGGAAGAGGAAAGAATAAAGGGCTTTTTTCTACTGAGGAGAGTTTGGCTGCAAGTGCTTATGATCATTTGACTATTGAGCATAACACTGACATGGGAGTCATAGACAACTTGCAGATTTCTCTTGAGAATGGGAAAGGGGAATCTACCCACCCCCAAGTCCAAACTTTGAGGGAGATGGTCATCTCTCACAAACCCGATGTGGTTTTTCTTTCCGAGACACATCAGTGTGAATCGTATATGGAAGATATCAGGAAGAATTTAGGTTACTGTAACTATTTTTATGTTGAACCGGTTAACACTGCCAGTGGTTTATGTAATTGGTGGAGTTCGGAGATCATTCTTGATTTGGTCAGTCATTCGAAGTTTTACATTGATTCTGTGGTTCATGGTCCAGATTGTGCAGATGGTGTTTTAATTAGCTGGCTATATGGTCCTCCCTACAGAGAAGATAAACCTAGGTTTTGGGACCATTGGTCTACTATTGCTAAATCTCGGTTAGATCCCTGGCTACTAATTGGTGATCTTAATGAGGTGCTTTGTCATTTTGAACGTGATGGTGACCATAATCCCTTGTTCTTTTCGACCAGATTGGGTACAAAGAAGAGACGTCCCTTGTTTCGGTTTGACGTCAATTGGGTTGATGATGCAGAAGCTAAACAATTGGTTGAGGAGTGCTGGACTATGACCCTGGGGTTGACAAACTTAGCAAACTGGACCTCTTATCTGGGTAGATGTCGATCCCAATTTAGAAGCTGGAGTAGGAAGAAGTTTCCTAAAGGCAACAAGGAACTTATCAAACAATGTCTTTTGGAATTGGATTGCTTACAGACTGACCAGCCTCTCACAAGTTTAGTATGTCAACTGGAATTGGAGTCAACTATCAGCACTTTGTGGATTCGAGAGGAGAAGCATTGGCATCAGAGGTCGAGGGTGAATTGGCTTCAGTCAGGTGATTCTAACACTCGGTTCTTCCATTTGTCTACCATTCAACGAAGGCAAAGAAACCAGATCCTAAAGATTCAAAGAGAAGATGGGTTGTGGATTAGTGGTGAAGCTCATGTTCGTTATGAATTTGAGACACAATTTGGTAATGTCTTTACCAGTTCGGGTACTCGAGATTGGGGCCAAGCCTTATCAGGAATTTCTCATTTGGTTTCTCCACAAATGAATCTTGATCTTTTGTCAGATATTTCCATTGATGAAGTTAGTGCAGCAGTCCATCAACTGGGTGCGCTAAAGGCGCTAGGCCCCGATGGATTTTTGAGTCTTTTCTATCATAAGTATTGGGACATTGTGCAACAGGTGGTATGGCAAACCTTGATGAACTTTTTTAATGGTTCTACTTGTTTACATGATCTCAACAGGACACACATTGTACTCATCCCTAAAGTTCCTAATCTTGAGAAGATGGGGCAGTTTCGTCCTATCAGTCTGTGTAATAATTCTTATACGATGTTATCGAAGATTTTGGCAAATAGGTTGAAACAGGTTCTGCCTCTCATCATCTCTCCAAACCAGAATGCTTTTGTTCCGGATCGACAAATACAAGATAACCTTATTGTAGCCCATGAGGCTTATCACTACCTCAAATTGAAGCGTGATGGAGGTAATCACGAGTTTGGGCTGAAACTAGACATGAACAAGGCTTATGATCGGGTTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

972

Amino Acids

110.5

Weight (kDa)

8.1

Isoelectric Point (pI)

39.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CCHC_4 PF14392 136 - 183 2.3e-10 Zinc knuckle
Exo_endo_phos PF03372 447 - 607 3.2e-06 Endonuclease/Exonuclease/phosphatase family
RVT_1 PF00078 879 - 971 9.3e-10 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000073)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g17821 FvH4_2g04610 FvH4_2g09611 FvH4_2g13563 FvH4_3g21493 FvH4_3g32115 FvH4_3g35060 FvH4_4g00220 FvH4_4g06410 FvH4_4g10367 FvH4_4g10367 FvH4_4g10367 FvH4_5g06101 FvH4_5g08842 FvH4_5g27366 FvH4_5g31283 FvH4_6g11611 FvH4_6g23032 FvH4_7g05661 FvH4_7g06172
malus_domestica MD13G1263700.v1.1
prunus_persica Prupe.1G292300_v2.0.a1 Prupe.5G009700_v2.0.a1 Prupe.6G158100_v2.0.a1 Prupe.8G025100_v2.0.a1 Prupe.8G042600_v2.0.a1
pyrus_communis pycom02g02250 pycom02g16030 pycom05g10490 pycom09g06450 pycom09g06460 pycom13g26200 pycom16g17700
rosa_chinensis RchiOBHm_Chr2g0163321 RchiOBHm_Chr4g0401981 RchiOBHm_Chr5g0005991 RchiOBHm_Chr5g0027191 RchiOBHm_Chr6g0249771
rosa_laevigata RLG00000001040 RLG00000001764 RLG00000002239 RLG00000002240 RLG00000003259 RLG00000008993 RLG00000013847 RLG00000014178 RLG00000015576 RLG00000017269 RLG00000017355 RLG00000018127 RLG00000018602 RLG00000018615 RLG00000019904 RLG00000020044 RLG00000021849 RLG00000021850 RLG00000027965 RLG00000029266 RLG00000029820 RLG00000031112 RLG00000031915 RLG00000033568 RLG00000035013 RLG00000035677 RLG00000036568 RLG00000036628
rosa_multiflora Rmu_co8254085.1_g000001 Rmu_co8462383.1_g000001 Rmu_sc0000185.1_g000049 Rmu_sc0000251.1_g000018 Rmu_sc0000367.1_g000094 Rmu_sc0000498.1_g000023 Rmu_sc0000868.1_g000005 Rmu_sc0001521.1_g000047 Rmu_sc0001534.1_g000007 Rmu_sc0002014.1_g000012 Rmu_sc0003214.1_g000009 Rmu_sc0003573.1_g000041 Rmu_sc0003832.1_g000023 Rmu_sc0004677.1_g000015 Rmu_sc0004967.1_g000014 Rmu_sc0005426.1_g000001 Rmu_sc0005621.1_g000004 Rmu_sc0005640.1_g000009 Rmu_sc0005947.1_g000034 Rmu_sc0006889.1_g000011 Rmu_sc0007121.1_g000014 Rmu_sc0009191.1_g000017 Rmu_sc0010556.1_g000011 Rmu_sc0011396.1_g000007 Rmu_sc0019862.1_g000002 Rmu_sc0022931.1_g000003 Rmu_sc0024164.1_g000001 Rmu_ssc0000391.1_g000027
rosa_roxburghii Rroxscaffold_1G00050060 Rroxscaffold_1G00061810 Rroxscaffold_2G00109590 Rroxscaffold_2G00113810 Rroxscaffold_2G00124040 Rroxscaffold_2G00143620 Rroxscaffold_2G00154760 Rroxscaffold_4G00302820 Rroxscaffold_4G00302830 Rroxscaffold_4G00308680 Rroxscaffold_4G00310240 Rroxscaffold_4G00327890 Rroxscaffold_5G00334600 Rroxscaffold_5G00336050 Rroxscaffold_5G00346560 Rroxscaffold_5G00347860 Rroxscaffold_5G00347870 Rroxscaffold_5G00349330 Rroxscaffold_5G00351980 Rroxscaffold_6G00395280 Rroxscaffold_6G00396750 Rroxscaffold_6G00402910 Rroxscaffold_7G00174790 Rroxscaffold_7G00177570 Rroxscaffold_7G00191820 Rroxscaffold_7G00203480 Rroxscaffold_7G00204380
rosa_rugosa Rorug01G0106600 Rorug01G0106600 Rorug01G0148500.1 Rorug02G0224200 Rorug02G0225500 Rorug02G0233000 Rorug02G0630400 Rorug03G0198000 Rorug03G0232600.1 Rorug03G0240500 Rorug04G0032900 Rorug04G0073300 Rorug04G0118500 Rorug04G0149600.1 Rorug04G0205100 Rorug04G0256700 Rorug05G0020200 Rorug05G0050000 Rorug05G0247300 Rorug05G0295100 Rorug05G0337900 Rorug05G0542600 Rorug06G0034800 Rorug06G0035100 Rorug06G0220100 Rorug07G0091900 Rorug07G0271200
rosa_samantha Rh1AG118200 Rh1AG253000 Rh1AG296300 Rh1AG409000 Rh1AG410600 Rh1BG073400 Rh1BG130400 Rh1BG147300 Rh1BG163600 Rh1CG016000 Rh1DG012200 Rh1DG012300 Rh1DG078600 Rh1DG078800 Rh1DG138100 Rh1DG179200 Rh1DG192600 Rh1DG194500 Rh1DG194600 Rh1DG214300 Rh1DG245900 Rh1DG267000 Rh1DG322700 Rh1DG442700 Rh2AG282000 Rh2AG423000 Rh2AG423100 Rh2AG460700 Rh2AG466200 Rh2AG513400 Rh2AG547000 Rh2BG277800 Rh2BG281200 Rh2BG298300 Rh2BG304300 Rh2BG433500 Rh2CG276000 Rh2CG282600 Rh2CG325500 Rh2CG393000 Rh2DG319200 Rh2DG443400 Rh3AG169300 Rh3AG303600 Rh3BG195000 Rh3BG282600 Rh3BG343400 Rh3BG343500 Rh3BG344100 Rh3CG111700 Rh3CG185700 Rh3CG259100 Rh3CG293000 Rh3CG317900 Rh3CG340900 Rh3CG341000 Rh3CG341100 Rh3CG354700 Rh3CG359200 Rh3CG368100 Rh3DG179900 Rh3DG288700 Rh3DG302600 Rh3DG316000 Rh3DG324500 Rh3DG324600 Rh3DG343200 Rh3DG343300 Rh3DG362400 Rh4AG071800 Rh4BG034000 Rh4BG044300 Rh4BG044400 Rh4BG069600 Rh4BG157400 Rh4CG007000 Rh4CG030400 Rh4CG037900 Rh4CG077700 Rh4CG115000 Rh4CG162400 Rh4CG162500 Rh4CG165500 Rh4CG165600 Rh4CG172900 Rh4CG239700 Rh4DG055900 Rh4DG173200 Rh5AG354900 Rh5AG511100 Rh5BG112600 Rh5BG541000 Rh5BG541100 Rh5BG549300 Rh5CG004400 Rh5CG315000 Rh5CG563800 Rh5CG572100 Rh5DG112200 Rh5DG191300 Rh5DG407600 Rh5DG407700 Rh5DG567500 Rh6AG002600 Rh6AG051400 Rh6AG199800 Rh6AG199900 Rh6AG206200 Rh6AG391000 Rh6AG391100 Rh6BG007500 Rh6BG053400 Rh6BG225900 Rh6BG227100 Rh6BG232400 Rh6CG091100 Rh6CG125400 Rh6CG186700 Rh6CG222700 Rh6CG222800 Rh6CG235500 Rh6CG247200 Rh6CG331200 Rh6CG465100 Rh6DG183500 Rh6DG213500 Rh6DG238800 Rh6DG263700 Rh7AG211800 Rh7AG441800 Rh7BG008600 Rh7BG253000 Rh7BG390000 Rh7CG189600 Rh7CG401500 Rh7CG424200 Rh7CG428700 Rh7CG479800 Rh7CG494300 Rh7DG177000 Rh7DG218700 Rh7DG254000 Rh7DG294800 Rh7DG407200 Rh7DG448800 Rh7DG461500
rosa_wichuraiana Rw0G012180 Rw1G005270 Rw1G012310 Rw1G016890 Rw1G021700 Rw2G014380 Rw2G027690 Rw3G016400 Rw3G028230 Rw3G029320 Rw4G002060 Rw4G002100 Rw4G003980 Rw4G014140 Rw4G014770 Rw4G014780 Rw4G015760 Rw4G021230 Rw5G000520 Rw5G035610 Rw6G001680 Rw6G017890 Rw6G027440 Rw6G034130 Rw6G034170 Rw7G012430 Rw7G033870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1346
AatII GACGTC 2 cut(s) 1861, 1881
AccB7I CCANNNNNTGG 1 cut(s) 2476
AccI GTMKAC 2 cut(s) 1727, 2231
AccIII TCCGGA 2 cut(s) 841, 2782
AciI CCGC 2 cut(s) 824, 1251
AclWI GGATC 6 cut(s) 353, 366, 1745, 1983, 2254, 2793
AcoI YGGCCR 2 cut(s) 3, 332
AcsI RAATTY 8 cut(s) 30, 490, 656, 727, 745, 1507, 2321, 2391
AcuI CTGAAG 3 cut(s) 536, 602, 2177
AcyI GRCGYC 2 cut(s) 1858, 1878
AdeI CACNNNGTG 1 cut(s) 2142
AfaI GTAC 4 cut(s) 838, 1846, 2362, 2628
AfiI CCNNNNNNNGG 5 cut(s) 397, 568, 1122, 1869, 2476
AgeI ACCGGT 1 cut(s) 1540
AhdI GACNNNNNGTC 1 cut(s) 1723
AjnI CCWGG 5 cut(s) 151, 334, 690, 1754, 1938
AleI CACNNNNGTG 2 cut(s) 513, 1474
Alw21I GWGCWC 1 cut(s) 1063
Alw26I GTCTC 3 cut(s) 1460, 1849, 2321
AlwI GGATC 6 cut(s) 353, 366, 1745, 1983, 2254, 2793
AlwNI CAGNNNCTG 2 cut(s) 995, 2743
Ama87I CYCGRG 1 cut(s) 2364
Aor13HI TCCGGA 2 cut(s) 841, 2782
AoxI GGCC 6 cut(s) 3, 332, 597, 945, 2374, 2497
ApeKI GCWGC 3 cut(s) 478, 1298, 2462
ApoI RAATTY 8 cut(s) 30, 490, 656, 727, 745, 1507, 2321, 2391
AsiGI ACCGGT 1 cut(s) 1540
AspA2I CCTAGG 1 cut(s) 1706
AspLEI GCGC 4 cut(s) 195, 471, 2485, 2494
AspS9I GGNCC 6 cut(s) 1640, 1682, 1717, 1965, 2374, 2498
AsuHPI GGTGA 7 cut(s) 241, 1198, 1781, 1820, 2194, 2213, 2312
AsuII TTCGAA 1 cut(s) 1610
AvaI CYCGRG 1 cut(s) 2364
AvaII GGWCC 4 cut(s) 1640, 1682, 1717, 1965
AvrII CCTAGG 1 cut(s) 1706
BalI TGGCCA 2 cut(s) 5, 334
BamHI GGATCC 1 cut(s) 358
BanII GRGCYC 1 cut(s) 1063
BauI CACGAG 1 cut(s) 2864
BbsI GAAGAC 2 cut(s) 608, 1203
Bbv12I GWGCWC 1 cut(s) 1063
BbvI GCAGC 3 cut(s) 465, 1285, 2474
BccI CCATC 9 cut(s) 1124, 1420, 1649, 1799, 2276, 2478, 2498, 2653, 2846
BciT130I CCWGG 5 cut(s) 153, 336, 692, 1756, 1940
BclI TGATCA 1 cut(s) 1312
BcoDI GTCTC 3 cut(s) 1460, 1849, 2321
BfaI CTAG 7 cut(s) 447, 750, 864, 1164, 1707, 2495, 2882
BfmI CTRYAG 3 cut(s) 479, 868, 1690
BfoI RGCGCY 2 cut(s) 196, 2495
BglI GCCNNNNNGGC 1 cut(s) 2822
BglII AGATCT 2 cut(s) 91, 207
BisI GCNGC 3 cut(s) 479, 1299, 2463
BlnI CCTAGG 1 cut(s) 1706
BlsI GCNGC 3 cut(s) 480, 1300, 2464
BmcAI AGTACT 1 cut(s) 838
Bme1390I CCNGG 5 cut(s) 153, 336, 692, 1756, 1940
Bme18I GGWCC 4 cut(s) 1640, 1682, 1717, 1965
BmeRI GACNNNNNGTC 1 cut(s) 1723
BmeT110I CYCGRG 1 cut(s) 2364
BmgT120I GGNCC 6 cut(s) 1640, 1682, 1717, 1965, 2374, 2498
BmiI GGNNCC 5 cut(s) 360, 971, 1718, 2375, 2500
BmrFI CCNGG 5 cut(s) 153, 336, 692, 1756, 1940
BmrI ACTGGG 2 cut(s) 209, 2486
BmsI GCATC 6 cut(s) 480, 546, 1093, 1138, 1885, 2176
BmuI ACTGGG 2 cut(s) 209, 2486
BpiI GAAGAC 2 cut(s) 608, 1203
BplI GAGNNNNNCTC 2 cut(s) 32, 64
BpmI CTGGAG 2 cut(s) 368, 2026
Bpu14I TTCGAA 1 cut(s) 1610
BpuEI CTTGAG 3 cut(s) 924, 1394, 2672
BsaBI GATNNNNATC 2 cut(s) 1430, 1987
BsaHI GRCGYC 2 cut(s) 1858, 1878
BsaJI CCNNGG 9 cut(s) 293, 600, 691, 923, 965, 1706, 1754, 1938, 1939
BsaWI WCCGGW 4 cut(s) 677, 841, 1540, 2782
Bsc4I CCNNNNNNNGG 5 cut(s) 397, 568, 1122, 1869, 2476
Bse118I RCCGGY 1 cut(s) 1540
Bse1I ACTGG 7 cut(s) 204, 1028, 1554, 1967, 2118, 2352, 2481
Bse8I GATNNNNATC 2 cut(s) 1430, 1987
BseAI TCCGGA 2 cut(s) 841, 2782
BseBI CCWGG 5 cut(s) 153, 336, 692, 1756, 1940
BseDI CCNNGG 9 cut(s) 293, 600, 691, 923, 965, 1706, 1754, 1938, 1939
BseGI GGATG 6 cut(s) 49, 387, 471, 770, 1212, 2631
BseJI GATNNNNATC 2 cut(s) 1430, 1987
BseLI CCNNNNNNNGG 5 cut(s) 397, 568, 1122, 1869, 2476
BseMII CTCAG 5 cut(s) 118, 249, 650, 1187, 1275
BseNI ACTGG 7 cut(s) 204, 1028, 1554, 1967, 2118, 2352, 2481
BseRI GAGGAG 3 cut(s) 1301, 1934, 2168
BseXI GCAGC 3 cut(s) 465, 1285, 2474
BsgI GTGCAG 3 cut(s) 108, 1671, 2481
Bsh1285I CGRYCG 1 cut(s) 678
BshFI GGCC 6 cut(s) 5, 334, 599, 947, 2376, 2499
BshTI ACCGGT 1 cut(s) 1540
BsiEI CGRYCG 1 cut(s) 678
BsiHKAI GWGCWC 1 cut(s) 1063
BsiHKCI CYCGRG 1 cut(s) 2364
BsiSI CCGG 5 cut(s) 475, 678, 842, 1541, 2783
BslFI GGGAC 4 cut(s) 216, 1730, 1845, 2552
BslI CCNNNNNNNGG 5 cut(s) 397, 568, 1122, 1869, 2476
BsmAI GTCTC 3 cut(s) 1460, 1849, 2321
BsmBI CGTCTC 1 cut(s) 1849
BsmFI GGGAC 4 cut(s) 216, 1730, 1845, 2552
BsmI GAATGC 1 cut(s) 2776
BsnI GGCC 6 cut(s) 5, 334, 599, 947, 2376, 2499
BsoBI CYCGRG 1 cut(s) 2364
Bsp119I TTCGAA 1 cut(s) 1610
Bsp1286I GDGCHC 1 cut(s) 1063
Bsp13I TCCGGA 2 cut(s) 841, 2782
Bsp19I CCATGG 1 cut(s) 293
BspACI CCGC 2 cut(s) 824, 1251
BspANI GGCC 6 cut(s) 5, 334, 599, 947, 2376, 2499
BspCNI CTCAG 5 cut(s) 117, 250, 649, 1188, 1276
BspEI TCCGGA 2 cut(s) 841, 2782
BspLI GGNNCC 5 cut(s) 360, 971, 1718, 2375, 2500
BspMAI CTGCAG 1 cut(s) 483
BspPI GGATC 6 cut(s) 353, 366, 1745, 1983, 2254, 2793
BspQI GCTCTTC 1 cut(s) 491
BspT104I TTCGAA 1 cut(s) 1610
BsrFI RCCGGY 1 cut(s) 1540
BsrI ACTGG 7 cut(s) 204, 1028, 1554, 1967, 2118, 2352, 2481
BssAI RCCGGY 1 cut(s) 1540
BssECI CCNNGG 9 cut(s) 293, 600, 691, 923, 965, 1706, 1754, 1938, 1939
BssNI GRCGYC 2 cut(s) 1858, 1878
BssSI CACGAG 1 cut(s) 2864
BssT1I CCWWGG 5 cut(s) 293, 600, 923, 965, 1706
Bst2BI CACGAG 1 cut(s) 2864
Bst2UI CCWGG 5 cut(s) 153, 336, 692, 1756, 1940
Bst4CI ACNGT 2 cut(s) 113, 1520
Bst6I CTCTTC 4 cut(s) 491, 1041, 1249, 1847
BstACI GRCGYC 2 cut(s) 1858, 1878
BstAPI GCANNNNNTGC 1 cut(s) 543
BstBI TTCGAA 1 cut(s) 1610
BstC8I GCNNGC 1 cut(s) 1673
BstDEI CTNAG 6 cut(s) 104, 258, 636, 1196, 1284, 1954
BstDSI CCRYGG 1 cut(s) 293
BstEII GGTNACC 1 cut(s) 1808
BstF5I GGATG 6 cut(s) 49, 387, 471, 770, 1212, 2631
BstH2I RGCGCY 2 cut(s) 196, 2495
BstHHI GCGC 4 cut(s) 195, 471, 2485, 2494
BstMAI GTCTC 3 cut(s) 1460, 1849, 2321
BstMCI CGRYCG 1 cut(s) 678
BstMWI GCNNNNNNNGC 8 cut(s) 475, 543, 605, 1248, 1304, 2489, 2491, 2822
BstNI CCWGG 5 cut(s) 153, 336, 692, 1756, 1940
BstNSI RCATGY 1 cut(s) 629
BstPI GGTNACC 1 cut(s) 1808
BstSCI CCNGG 5 cut(s) 151, 334, 690, 1754, 1938
BstSFI CTRYAG 3 cut(s) 479, 868, 1690
BstV1I GCAGC 3 cut(s) 465, 1285, 2474
BstV2I GAAGAC 2 cut(s) 608, 1203
BstX2I RGATCY 5 cut(s) 91, 207, 358, 1750, 2259
BstYI RGATCY 5 cut(s) 91, 207, 358, 1750, 2259
BsuRI GGCC 6 cut(s) 5, 334, 599, 947, 2376, 2499
BtgI CCRYGG 1 cut(s) 293
BtsCI GGATG 6 cut(s) 49, 387, 471, 770, 1212, 2631
BtsI GCAGTG 2 cut(s) 895, 1548
BtsIMutI CAGTG 8 cut(s) 112, 197, 348, 895, 1335, 1481, 1548, 1561
Cac8I GCNNGC 1 cut(s) 1673
CaiI CAGNNNCTG 2 cut(s) 995, 2743
CfoI GCGC 4 cut(s) 195, 471, 2485, 2494
Cfr10I RCCGGY 1 cut(s) 1540
Cfr13I GGNCC 6 cut(s) 1640, 1682, 1717, 1965, 2374, 2498
Csp6I GTAC 4 cut(s) 837, 1845, 2361, 2627
CspAI ACCGGT 1 cut(s) 1540
CviQI GTAC 4 cut(s) 837, 1845, 2361, 2627
DdeI CTNAG 6 cut(s) 104, 258, 636, 1196, 1284, 1954
DraIII CACNNNGTG 1 cut(s) 2142
DrdI GACNNNNNNGTC 1 cut(s) 1346
DriI GACNNNNNGTC 1 cut(s) 1723
DseDI GACNNNNNNGTC 1 cut(s) 1346
EaeI YGGCCR 2 cut(s) 3, 332
Eam1104I CTCTTC 4 cut(s) 491, 1041, 1249, 1847
Eam1105I GACNNNNNGTC 1 cut(s) 1723
EarI CTCTTC 4 cut(s) 491, 1041, 1249, 1847
Ecl136II GAGCTC 1 cut(s) 1061
Eco130I CCWWGG 5 cut(s) 293, 600, 923, 965, 1706
Eco147I AGGCCT 2 cut(s) 599, 947
Eco24I GRGCYC 1 cut(s) 1063
Eco32I GATATC 1 cut(s) 1498
Eco47I GGWCC 4 cut(s) 1640, 1682, 1717, 1965
Eco53kI GAGCTC 1 cut(s) 1061
Eco57I CTGAAG 3 cut(s) 536, 602, 2177
Eco88I CYCGRG 1 cut(s) 2364
Eco91I GGTNACC 1 cut(s) 1808
EcoICRI GAGCTC 1 cut(s) 1061
EcoO109I RGGNCCY 1 cut(s) 2498
EcoO65I GGTNACC 1 cut(s) 1808
EcoRII CCWGG 5 cut(s) 151, 334, 690, 1754, 1938
EcoRV GATATC 1 cut(s) 1498
EcoT14I CCWWGG 5 cut(s) 293, 600, 923, 965, 1706
EcoT38I GRGCYC 1 cut(s) 1063
ErhI CCWWGG 5 cut(s) 293, 600, 923, 965, 1706
Esp3I CGTCTC 1 cut(s) 1849
FalI AAGNNNNNCTT 6 cut(s) 406, 438, 1195, 1227, 2790, 2822
FaqI GGGAC 4 cut(s) 216, 1730, 1845, 2552
FbaI TGATCA 1 cut(s) 1312
FblI GTMKAC 2 cut(s) 1727, 2231
Fnu4HI GCNGC 3 cut(s) 479, 1299, 2463
FokI GGATG 6 cut(s) 56, 374, 458, 777, 1219, 2618
FriOI GRGCYC 1 cut(s) 1063
Fsp4HI GCNGC 3 cut(s) 479, 1299, 2463
FspBI CTAG 7 cut(s) 447, 750, 864, 1164, 1707, 2495, 2882
GlaI GCGC 4 cut(s) 194, 470, 2484, 2493
GluI GCNGC 3 cut(s) 479, 1299, 2463
GsuI CTGGAG 2 cut(s) 368, 2026
HaeII RGCGCY 2 cut(s) 196, 2495
HaeIII GGCC 6 cut(s) 5, 334, 599, 947, 2376, 2499
HapII CCGG 5 cut(s) 475, 678, 842, 1541, 2783
HhaI GCGC 4 cut(s) 195, 471, 2485, 2494
Hin1I GRCGYC 2 cut(s) 1858, 1878
Hin6I GCGC 4 cut(s) 193, 469, 2483, 2492
HinP1I GCGC 4 cut(s) 193, 469, 2483, 2492
HincII GTYRAC 5 cut(s) 850, 1546, 1947, 2111, 2127
HindII GTYRAC 5 cut(s) 850, 1546, 1947, 2111, 2127
HindIII AAGCTT 2 cut(s) 275, 913
HpaI GTTAAC 2 cut(s) 850, 1546
HpaII CCGG 5 cut(s) 475, 678, 842, 1541, 2783
HphI GGTGA 7 cut(s) 241, 1198, 1781, 1820, 2194, 2213, 2312
HpyAV CCTTC 4 cut(s) 298, 1145, 1224, 2238
HpyCH4III ACNGT 2 cut(s) 113, 1520
HpyCH4IV ACGT 5 cut(s) 300, 644, 1801, 1858, 1878
HpyF10VI GCNNNNNNNGC 8 cut(s) 475, 543, 605, 1248, 1304, 2489, 2491, 2822
HpyF3I CTNAG 6 cut(s) 104, 258, 636, 1196, 1284, 1954
HpySE526I ACGT 5 cut(s) 300, 644, 1801, 1858, 1878
Hsp92I GRCGYC 2 cut(s) 1858, 1878
HspAI GCGC 4 cut(s) 193, 469, 2483, 2492
Kpn2I TCCGGA 2 cut(s) 841, 2782
Ksp22I TGATCA 1 cut(s) 1312
KspAI GTTAAC 2 cut(s) 850, 1546
LguI GCTCTTC 1 cut(s) 491
Lsp1109I GCAGC 3 cut(s) 465, 1285, 2474
LweI GCATC 6 cut(s) 480, 546, 1093, 1138, 1885, 2176
MaeI CTAG 7 cut(s) 447, 750, 864, 1164, 1707, 2495, 2882
MaeII ACGT 5 cut(s) 300, 644, 1801, 1858, 1878
MaeIII GTNAC 7 cut(s) 107, 460, 506, 767, 1514, 1520, 1808
MfeI CAATTG 3 cut(s) 511, 1882, 1910
MflI RGATCY 5 cut(s) 91, 207, 358, 1750, 2259
MhlI GDGCHC 1 cut(s) 1063
MlsI TGGCCA 2 cut(s) 5, 334
MluNI TGGCCA 2 cut(s) 5, 334
MmeI TCCRAC 1 cut(s) 674
Mox20I TGGCCA 2 cut(s) 5, 334
MroI TCCGGA 2 cut(s) 841, 2782
MscI TGGCCA 2 cut(s) 5, 334
MseI TTAA 8 cut(s) 438, 849, 1017, 1202, 1545, 1664, 1776, 2583
MslI CAYNNNNRTG 4 cut(s) 513, 1340, 1474, 1476
Msp20I TGGCCA 2 cut(s) 5, 334
MspI CCGG 5 cut(s) 475, 678, 842, 1541, 2783
MspR9I CCNGG 5 cut(s) 153, 336, 692, 1756, 1940
MunI CAATTG 3 cut(s) 511, 1882, 1910
Mva1269I GAATGC 1 cut(s) 2776
MvaI CCWGG 5 cut(s) 153, 336, 692, 1756, 1940
MwoI GCNNNNNNNGC 8 cut(s) 475, 543, 605, 1248, 1304, 2489, 2491, 2822
NcoI CCATGG 1 cut(s) 293
NlaIV GGNNCC 5 cut(s) 360, 971, 1718, 2375, 2500
NmeAIII GCCGAG 1 cut(s) 634
NmuCI GTSAC 4 cut(s) 107, 506, 767, 1808
NspI RCATGY 1 cut(s) 629
NspV TTCGAA 1 cut(s) 1610
OliI CACNNNNGTG 2 cut(s) 513, 1474
PaeR7I CTCGAG 1 cut(s) 2364
PasI CCCWGGG 1 cut(s) 1939
PceI AGGCCT 2 cut(s) 599, 947
PciSI GCTCTTC 1 cut(s) 491
PcsI WCGNNNNNNNCGW 1 cut(s) 2708
PctI GAATGC 1 cut(s) 2776
PfeI GAWTC 9 cut(s) 681, 1036, 1391, 1481, 1625, 2146, 2204, 2269, 2419
PflMI CCANNNNNTGG 1 cut(s) 2476
PinAI ACCGGT 1 cut(s) 1540
PkrI GCNGC 3 cut(s) 480, 1300, 2464
Psp124BI GAGCTC 1 cut(s) 1063
Psp6I CCWGG 5 cut(s) 151, 334, 690, 1754, 1938
PspEI GGTNACC 1 cut(s) 1808
PspGI CCWGG 5 cut(s) 151, 334, 690, 1754, 1938
PspN4I GGNNCC 5 cut(s) 360, 971, 1718, 2375, 2500
PspPI GGNCC 6 cut(s) 1640, 1682, 1717, 1965, 2374, 2498
PstI CTGCAG 1 cut(s) 483
PstNI CAGNNNCTG 2 cut(s) 995, 2743
PsuI RGATCY 5 cut(s) 91, 207, 358, 1750, 2259
RsaI GTAC 4 cut(s) 838, 1846, 2362, 2628
RsaNI GTAC 4 cut(s) 837, 1845, 2361, 2627
RseI CAYNNNNRTG 4 cut(s) 513, 1340, 1474, 1476
SacI GAGCTC 1 cut(s) 1063
SapI GCTCTTC 1 cut(s) 491
SaqAI TTAA 8 cut(s) 438, 849, 1017, 1202, 1545, 1664, 1776, 2583
SatI GCNGC 3 cut(s) 479, 1299, 2463
Sau96I GGNCC 6 cut(s) 1640, 1682, 1717, 1965, 2374, 2498
ScaI AGTACT 1 cut(s) 838
ScrFI CCNGG 5 cut(s) 153, 336, 692, 1756, 1940
SduI GDGCHC 1 cut(s) 1063
SfaNI GCATC 6 cut(s) 480, 546, 1093, 1138, 1885, 2176
SfcI CTRYAG 3 cut(s) 479, 868, 1690
Sfr274I CTCGAG 1 cut(s) 2364
SfuI TTCGAA 1 cut(s) 1610
SinI GGWCC 4 cut(s) 1640, 1682, 1717, 1965
SlaI CTCGAG 1 cut(s) 2364
SmiMI CAYNNNNRTG 4 cut(s) 513, 1340, 1474, 1476
SmlI CTYRAG 4 cut(s) 939, 1373, 2364, 2651
SmoI CTYRAG 4 cut(s) 939, 1373, 2364, 2651
SseBI AGGCCT 2 cut(s) 599, 947
SsiI CCGC 2 cut(s) 824, 1251
SspMI CTAG 7 cut(s) 447, 750, 864, 1164, 1707, 2495, 2882
SstI GAGCTC 1 cut(s) 1063
StuI AGGCCT 2 cut(s) 599, 947
StyD4I CCNGG 5 cut(s) 151, 334, 690, 1754, 1938
StyI CCWWGG 5 cut(s) 293, 600, 923, 965, 1706
TaaI ACNGT 2 cut(s) 113, 1520
TaiI ACGT 5 cut(s) 303, 647, 1804, 1861, 1881
TaqI TCGA 8 cut(s) 1610, 1832, 1987, 2149, 2177, 2365, 2711, 2788
TatI WGTACW 2 cut(s) 836, 2626
TfiI GAWTC 9 cut(s) 681, 1036, 1391, 1481, 1625, 2146, 2204, 2269, 2419
Tru1I TTAA 8 cut(s) 438, 849, 1017, 1202, 1545, 1664, 1776, 2583
Tru9I TTAA 8 cut(s) 438, 849, 1017, 1202, 1545, 1664, 1776, 2583
TscAI CASTG 8 cut(s) 112, 204, 348, 895, 1342, 1481, 1555, 1561
TseFI GTSAC 4 cut(s) 107, 506, 767, 1808
TseI GCWGC 3 cut(s) 478, 1298, 2462
Tsp45I GTSAC 4 cut(s) 107, 506, 767, 1808
TspDTI ATGAA 9 cut(s) 376, 501, 1028, 1625, 2334, 2432, 2466, 2588, 2903
TspGWI ACGGA 2 cut(s) 380, 1138
TspRI CASTG 8 cut(s) 112, 204, 348, 895, 1342, 1481, 1555, 1561
Van91I CCANNNNNTGG 1 cut(s) 2476
VpaK11BI GGWCC 4 cut(s) 1640, 1682, 1717, 1965
XapI RAATTY 8 cut(s) 30, 490, 656, 727, 745, 1507, 2321, 2391
XceI RCATGY 1 cut(s) 629
XhoI CTCGAG 1 cut(s) 2364
XmaJI CCTAGG 1 cut(s) 1706
XmiI GTMKAC 2 cut(s) 1727, 2231
XspI CTAG 7 cut(s) 447, 750, 864, 1164, 1707, 2495, 2882
ZraI GACGTC 2 cut(s) 1859, 1879
ZrmI AGTACT 1 cut(s) 838
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.