Domain of unknown function (DUF4283)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 1 cut(s) | 359 |
| AciI | CCGC | 3 cut(s) | 214, 359, 461 |
| AclWI | GGATC | 3 cut(s) | 197, 361, 374 |
| AcoI | YGGCCR | 1 cut(s) | 622 |
| AcsI | RAATTY | 2 cut(s) | 268, 581 |
| AfaI | GTAC | 1 cut(s) | 481 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 376, 574 |
| AflIII | ACRYGT | 1 cut(s) | 141 |
| AgsI | TTSAA | 7 cut(s) | 148, 236, 308, 467, 581, 593, 641 |
| AhlI | ACTAGT | 1 cut(s) | 716 |
| AjnI | CCWGG | 3 cut(s) | 155, 188, 572 |
| AleI | CACNNNNGTG | 1 cut(s) | 435 |
| AluBI | AGCT | 4 cut(s) | 8, 95, 251, 340 |
| AluI | AGCT | 4 cut(s) | 8, 95, 251, 340 |
| AlwI | GGATC | 3 cut(s) | 197, 361, 374 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 230 |
| AoxI | GGCC | 4 cut(s) | 314, 524, 622, 680 |
| ApeKI | GCWGC | 3 cut(s) | 92, 248, 340 |
| ApoI | RAATTY | 2 cut(s) | 268, 581 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 203, 235 |
| AspS9I | GGNCC | 2 cut(s) | 73, 115 |
| AsuHPI | GGTGA | 2 cut(s) | 264, 449 |
| AsuII | TTCGAA | 1 cut(s) | 64 |
| AvaII | GGWCC | 2 cut(s) | 73, 115 |
| BamHI | GGATCC | 1 cut(s) | 366 |
| BbvI | GCAGC | 3 cut(s) | 104, 260, 327 |
| BccI | CCATC | 2 cut(s) | 554, 653 |
| BceAI | ACGGC | 1 cut(s) | 609 |
| BciT130I | CCWGG | 3 cut(s) | 157, 190, 574 |
| BcuI | ACTAGT | 1 cut(s) | 716 |
| BfaI | CTAG | 3 cut(s) | 252, 519, 717 |
| BglII | AGATCT | 1 cut(s) | 28 |
| BisI | GCNGC | 3 cut(s) | 93, 249, 341 |
| BlsI | GCNGC | 3 cut(s) | 94, 250, 342 |
| Bme1390I | CCNGG | 3 cut(s) | 157, 190, 574 |
| Bme18I | GGWCC | 2 cut(s) | 73, 115 |
| BmgT120I | GGNCC | 2 cut(s) | 73, 115 |
| BmiI | GGNNCC | 2 cut(s) | 117, 368 |
| BmrFI | CCNGG | 3 cut(s) | 157, 190, 574 |
| BmsI | GCATC | 2 cut(s) | 170, 505 |
| Bpu14I | TTCGAA | 1 cut(s) | 64 |
| BsaJI | CCNNGG | 6 cut(s) | 69, 156, 189, 275, 573, 677 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 376, 574 |
| Bse1I | ACTGG | 1 cut(s) | 541 |
| BseBI | CCWGG | 3 cut(s) | 157, 190, 574 |
| BseDI | CCNNGG | 6 cut(s) | 69, 156, 189, 275, 573, 677 |
| BseGI | GGATG | 3 cut(s) | 199, 645, 714 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 376, 574 |
| BseMII | CTCAG | 1 cut(s) | 309 |
| BseNI | ACTGG | 1 cut(s) | 541 |
| BseRI | GAGGAG | 1 cut(s) | 350 |
| BseXI | GCAGC | 3 cut(s) | 104, 260, 327 |
| Bsh1236I | CGCG | 1 cut(s) | 359 |
| Bsh1285I | CGRYCG | 2 cut(s) | 80, 734 |
| BshFI | GGCC | 4 cut(s) | 316, 526, 624, 682 |
| BsiEI | CGRYCG | 2 cut(s) | 80, 734 |
| BsiSI | CCGG | 1 cut(s) | 727 |
| BslFI | GGGAC | 1 cut(s) | 101 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 376, 574 |
| BsmFI | GGGAC | 1 cut(s) | 101 |
| BsmI | GAATGC | 1 cut(s) | 722 |
| BsnI | GGCC | 4 cut(s) | 316, 526, 624, 682 |
| Bsp119I | TTCGAA | 1 cut(s) | 64 |
| Bsp143I | GATC | 4 cut(s) | 28, 77, 202, 366 |
| Bsp19I | CCATGG | 1 cut(s) | 275 |
| BspACI | CCGC | 3 cut(s) | 214, 359, 461 |
| BspANI | GGCC | 4 cut(s) | 316, 526, 624, 682 |
| BspCNI | CTCAG | 1 cut(s) | 308 |
| BspFNI | CGCG | 1 cut(s) | 359 |
| BspLI | GGNNCC | 2 cut(s) | 117, 368 |
| BspPI | GGATC | 3 cut(s) | 197, 361, 374 |
| BspT104I | TTCGAA | 1 cut(s) | 64 |
| BsrI | ACTGG | 1 cut(s) | 541 |
| BssECI | CCNNGG | 6 cut(s) | 69, 156, 189, 275, 573, 677 |
| BssMI | GATC | 4 cut(s) | 28, 77, 202, 366 |
| BssT1I | CCWWGG | 3 cut(s) | 69, 275, 677 |
| Bst2UI | CCWGG | 3 cut(s) | 157, 190, 574 |
| Bst4CI | ACNGT | 3 cut(s) | 232, 668, 735 |
| Bst6I | CTCTTC | 1 cut(s) | 552 |
| BstBI | TTCGAA | 1 cut(s) | 64 |
| BstC8I | GCNNGC | 1 cut(s) | 90 |
| BstDEI | CTNAG | 1 cut(s) | 295 |
| BstDSI | CCRYGG | 1 cut(s) | 275 |
| BstF5I | GGATG | 3 cut(s) | 199, 645, 714 |
| BstFNI | CGCG | 1 cut(s) | 359 |
| BstKTI | GATC | 4 cut(s) | 31, 80, 205, 369 |
| BstMBI | GATC | 4 cut(s) | 28, 77, 202, 366 |
| BstMCI | CGRYCG | 2 cut(s) | 80, 734 |
| BstNI | CCWGG | 3 cut(s) | 157, 190, 574 |
| BstNSI | RCATGY | 1 cut(s) | 145 |
| BstSCI | CCNGG | 3 cut(s) | 155, 188, 572 |
| BstUI | CGCG | 1 cut(s) | 359 |
| BstV1I | GCAGC | 3 cut(s) | 104, 260, 327 |
| BstX2I | RGATCY | 3 cut(s) | 28, 202, 366 |
| BstYI | RGATCY | 3 cut(s) | 28, 202, 366 |
| BsuRI | GGCC | 4 cut(s) | 316, 526, 624, 682 |
| BtgI | CCRYGG | 1 cut(s) | 275 |
| BtgZI | GCGATG | 1 cut(s) | 340 |
| BtsCI | GGATG | 3 cut(s) | 199, 645, 714 |
| BtsI | GCAGTG | 1 cut(s) | 183 |
| BtsIMutI | CAGTG | 5 cut(s) | 183, 207, 228, 534, 673 |
| Cac8I | GCNNGC | 1 cut(s) | 90 |
| CaiI | CAGNNNCTG | 1 cut(s) | 230 |
| Cfr13I | GGNCC | 2 cut(s) | 73, 115 |
| Csp6I | GTAC | 1 cut(s) | 480 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 118, 153 |
| CviAII | CATG | 2 cut(s) | 142, 276 |
| CviQI | GTAC | 1 cut(s) | 480 |
| DdeI | CTNAG | 1 cut(s) | 295 |
| DpnI | GATC | 4 cut(s) | 30, 79, 204, 368 |
| DpnII | GATC | 4 cut(s) | 28, 77, 202, 366 |
| EaeI | YGGCCR | 1 cut(s) | 622 |
| Eam1104I | CTCTTC | 1 cut(s) | 552 |
| EarI | CTCTTC | 1 cut(s) | 552 |
| Eco130I | CCWWGG | 3 cut(s) | 69, 275, 677 |
| Eco47I | GGWCC | 2 cut(s) | 73, 115 |
| EcoO109I | RGGNCCY | 1 cut(s) | 115 |
| EcoRI | GAATTC | 1 cut(s) | 268 |
| EcoRII | CCWGG | 3 cut(s) | 155, 188, 572 |
| EcoT14I | CCWWGG | 3 cut(s) | 69, 275, 677 |
| ErhI | CCWWGG | 3 cut(s) | 69, 275, 677 |
| FaeI | CATG | 2 cut(s) | 145, 279 |
| FaiI | YATR | 8 cut(s) | 123, 143, 260, 277, 391, 393, 395, 699 |
| FaqI | GGGAC | 1 cut(s) | 101 |
| FatI | CATG | 2 cut(s) | 141, 275 |
| FauI | CCCGC | 1 cut(s) | 468 |
| Fnu4HI | GCNGC | 3 cut(s) | 93, 249, 341 |
| FokI | GGATG | 3 cut(s) | 206, 632, 721 |
| Fsp4HI | GCNGC | 3 cut(s) | 93, 249, 341 |
| FspBI | CTAG | 3 cut(s) | 252, 519, 717 |
| GluI | GCNGC | 3 cut(s) | 93, 249, 341 |
| HaeIII | GGCC | 4 cut(s) | 316, 526, 624, 682 |
| HapII | CCGG | 1 cut(s) | 727 |
| Hin1II | CATG | 2 cut(s) | 145, 279 |
| HinfI | GANTC | 4 cut(s) | 180, 498, 540, 606 |
| HpaII | CCGG | 1 cut(s) | 727 |
| HphI | GGTGA | 2 cut(s) | 264, 449 |
| Hpy166II | GTNNAC | 1 cut(s) | 301 |
| Hpy188I | TCNGA | 2 cut(s) | 41, 407 |
| Hpy188III | TCNNGA | 3 cut(s) | 370, 603, 649 |
| Hpy8I | GTNNAC | 1 cut(s) | 301 |
| HpyAV | CCTTC | 5 cut(s) | 71, 470, 503, 516, 680 |
| HpyCH4III | ACNGT | 3 cut(s) | 232, 668, 735 |
| HpyCH4V | TGCA | 4 cut(s) | 92, 107, 224, 722 |
| HpyF3I | CTNAG | 1 cut(s) | 295 |
| Hsp92II | CATG | 2 cut(s) | 145, 279 |
| Kzo9I | GATC | 4 cut(s) | 28, 77, 202, 366 |
| LmnI | GCTCC | 1 cut(s) | 337 |
| Lsp1109I | GCAGC | 3 cut(s) | 104, 260, 327 |
| LweI | GCATC | 2 cut(s) | 170, 505 |
| MaeI | CTAG | 3 cut(s) | 252, 519, 717 |
| MaeIII | GTNAC | 2 cut(s) | 226, 290 |
| MalI | GATC | 4 cut(s) | 30, 79, 204, 368 |
| MboI | GATC | 4 cut(s) | 28, 77, 202, 366 |
| MboII | GAAGA | 4 cut(s) | 160, 428, 539, 683 |
| MflI | RGATCY | 3 cut(s) | 28, 202, 366 |
| MluCI | AATT | 4 cut(s) | 268, 282, 310, 581 |
| MlyI | GAGTC | 1 cut(s) | 507 |
| MnlI | CCTC | 4 cut(s) | 235, 328, 369, 580 |
| MseI | TTAA | 2 cut(s) | 285, 585 |
| MslI | CAYNNNNRTG | 3 cut(s) | 140, 435, 449 |
| MspA1I | CMGCKG | 1 cut(s) | 95 |
| MspI | CCGG | 1 cut(s) | 727 |
| MspR9I | CCNGG | 3 cut(s) | 157, 190, 574 |
| Mva1269I | GAATGC | 1 cut(s) | 722 |
| MvaI | CCWGG | 3 cut(s) | 157, 190, 574 |
| MvnI | CGCG | 1 cut(s) | 359 |
| NcoI | CCATGG | 1 cut(s) | 275 |
| NdeII | GATC | 4 cut(s) | 28, 77, 202, 366 |
| NlaIII | CATG | 2 cut(s) | 145, 279 |
| NlaIV | GGNNCC | 2 cut(s) | 117, 368 |
| NmuCI | GTSAC | 1 cut(s) | 226 |
| NspI | RCATGY | 1 cut(s) | 145 |
| NspV | TTCGAA | 1 cut(s) | 64 |
| OliI | CACNNNNGTG | 1 cut(s) | 435 |
| PciI | ACATGT | 1 cut(s) | 141 |
| PctI | GAATGC | 1 cut(s) | 722 |
| PfeI | GAWTC | 3 cut(s) | 180, 540, 606 |
| PkrI | GCNGC | 3 cut(s) | 94, 250, 342 |
| Ple19I | CGATCG | 1 cut(s) | 80 |
| PleI | GAGTC | 1 cut(s) | 506 |
| PpsI | GAGTC | 1 cut(s) | 506 |
| PpuMI | RGGWCCY | 1 cut(s) | 115 |
| PscI | ACATGT | 1 cut(s) | 141 |
| Psp5II | RGGWCCY | 1 cut(s) | 115 |
| Psp6I | CCWGG | 3 cut(s) | 155, 188, 572 |
| PspGI | CCWGG | 3 cut(s) | 155, 188, 572 |
| PspN4I | GGNNCC | 2 cut(s) | 117, 368 |
| PspPI | GGNCC | 2 cut(s) | 73, 115 |
| PspPPI | RGGWCCY | 1 cut(s) | 115 |
| PstNI | CAGNNNCTG | 1 cut(s) | 230 |
| PsuI | RGATCY | 3 cut(s) | 28, 202, 366 |
| PvuI | CGATCG | 1 cut(s) | 80 |
| PvuII | CAGCTG | 1 cut(s) | 95 |
| RsaI | GTAC | 1 cut(s) | 481 |
| RsaNI | GTAC | 1 cut(s) | 480 |
| RseI | CAYNNNNRTG | 3 cut(s) | 140, 435, 449 |
| SaqAI | TTAA | 2 cut(s) | 285, 585 |
| SatI | GCNGC | 3 cut(s) | 93, 249, 341 |
| Sau3AI | GATC | 4 cut(s) | 28, 77, 202, 366 |
| Sau96I | GGNCC | 2 cut(s) | 73, 115 |
| SchI | GAGTC | 1 cut(s) | 507 |
| ScrFI | CCNGG | 3 cut(s) | 157, 190, 574 |
| SetI | ASST | 8 cut(s) | 10, 71, 97, 117, 253, 327, 342, 481 |
| SfaNI | GCATC | 2 cut(s) | 170, 505 |
| SfuI | TTCGAA | 1 cut(s) | 64 |
| SinI | GGWCC | 2 cut(s) | 73, 115 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 140, 435, 449 |
| SpeI | ACTAGT | 1 cut(s) | 716 |
| Sse9I | AATT | 4 cut(s) | 268, 282, 310, 581 |
| SsiI | CCGC | 3 cut(s) | 214, 359, 461 |
| SspI | AATATT | 1 cut(s) | 102 |
| SspMI | CTAG | 3 cut(s) | 252, 519, 717 |
| StyD4I | CCNGG | 3 cut(s) | 155, 188, 572 |
| StyI | CCWWGG | 3 cut(s) | 69, 275, 677 |
| TaaI | ACNGT | 3 cut(s) | 232, 668, 735 |
| TaqI | TCGA | 3 cut(s) | 64, 496, 604 |
| TaqII | GACCGA | 1 cut(s) | 90 |
| TasI | AATT | 4 cut(s) | 268, 282, 310, 581 |
| TfiI | GAWTC | 3 cut(s) | 180, 540, 606 |
| Tru1I | TTAA | 2 cut(s) | 285, 585 |
| Tru9I | TTAA | 2 cut(s) | 285, 585 |
| TscAI | CASTG | 5 cut(s) | 190, 214, 235, 541, 673 |
| TseFI | GTSAC | 1 cut(s) | 226 |
| TseI | GCWGC | 3 cut(s) | 92, 248, 340 |
| Tsp45I | GTSAC | 1 cut(s) | 226 |
| TspDTI | ATGAA | 1 cut(s) | 433 |
| TspGWI | ACGGA | 1 cut(s) | 491 |
| TspRI | CASTG | 5 cut(s) | 190, 214, 235, 541, 673 |
| VpaK11BI | GGWCC | 2 cut(s) | 73, 115 |
| XapI | RAATTY | 2 cut(s) | 268, 581 |
| XceI | RCATGY | 1 cut(s) | 145 |
| XspI | CTAG | 3 cut(s) | 252, 519, 717 |