Rw1G005270

Domain of unknown function (DUF4283)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000073
Physical Location & Seq
Forward (+)
10200914 .. 10202254
1341 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G005270.1

Sequence Viewer

Length: 1341 bp
ATGGAACCAGATCACATTGGCAATCCAAACCCAAACTCAGATAATCCAAACCTGGCCAAACCAAATCCTGCTGATCAGGTTCTGGACCTCAATCAACCTGACCCTCATGCTGATCAACCTCTTACTCTTCTCGGCTTTCTGATAGCTGACAAATTTCTCAATGTTGCTGCTGTGATCCGCATCCTCACTCGAGCCTGGTCTGAGTTTGGCGAGGTTCATATCGAGGCTATCCGTGACCACCGTACCTTCACCATTACACCAATGGAGTGGGAAACTGGACAGCAAATCCTTAATCGTGGTCCCTGGACTGTTATGGGACATACAATTTGCATCAACATTTGGCCCCTTGATGCTAGACTAGAGGATGTTCCGGTCCACCAGCTGGCTATCTGGATCCAAGCCCATGGTCTCCCGCGAGGTCAGATGACTCATGCCAATGCTCAGCTGCTGGGTGCTGTTGCCGGTACCGTTCTTTCGGTGGAGAATTCCAACACGGTCAATGGTTGGAGGGGTTTTGTCCGAGTCCGCGTGAACATCAATACCCAAAACCCACTGGTTCCGGGTGTCTGGATCAGGTGTGGATCTAACCCTCCATTGTGGGTGGAGTTTAGATATGAGAGGATTTCTCTCTTCTGCTATAACTGTGGCCGCCTCAGTCATAGTGAACGTTTTTGCAATCAACCTCGGTCCCCTGGAGCTGAGAGACTGGGAGTTTGGATGATTACTCCTGCGGCTAGACGTCTTACTTCAGGCCCCCAACCTGATGGAGAGCGAGGTTGGGATAGGTTCCAGAGACAATGCTTCCAATCTCGACAAAATAGCCAACAGTATTCTGCCTCAAACCCATCTACCCAACCCCTTCTTCAAGCTGCGAGTCAGACATTTTTCAGGGGTCCACCACAGTACCGATCCACTCTGGTCTTATCTGAGAAGTCCCCTTCACCTGTGAGGGACGCCCCCACACCTGATTTGCCTCCAACCACCTTTGTCGCCTCCTCCTCTTCCGGTCCCCGGGATAAAGGGAAGGCAAAGTTGTTTCCGGCGGAGGATTTAATTGTGAATAATGTTGATTTTGCAGAGCCGTGTTTGCCTAATGGATCAGACCAAACTCTATACCCAACCTTGGGCCTTTCGGGGGAAATAAATTTGGGCCACACTCAGTTCTTTTTTGACAACTCTCTCAGTGAGTTTGTTCCGATCCCTACACCACTACAACCTTATCCTTTCAATCATACCCAAACCCCACTTCATACCCTTTACCCACCTTTTCATCTACCTCCCACACCAAACTCTCAGTCTTATGCAAAGGATTCTCTGGAAGCTTTGGTGGCTCCGGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 zf-CCHC_4 zf-RING_2

Protein Analysis

446

Amino Acids

49.69

Weight (kDa)

6.12

Isoelectric Point (pI)

40.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4283 PF14111 42 - 118 3e-06 Domain of unknown function (DUF4283)
zf-CCHC_4 PF14392 184 - 226 2.2e-12 Zinc knuckle
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000073)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g17821 FvH4_2g04610 FvH4_2g09611 FvH4_2g13563 FvH4_3g21493 FvH4_3g32115 FvH4_3g35060 FvH4_4g00220 FvH4_4g06410 FvH4_4g10367 FvH4_4g10367 FvH4_4g10367 FvH4_5g06101 FvH4_5g08842 FvH4_5g27366 FvH4_5g31283 FvH4_6g11611 FvH4_6g23032 FvH4_7g05661 FvH4_7g06172
malus_domestica MD13G1263700.v1.1
prunus_persica Prupe.1G292300_v2.0.a1 Prupe.5G009700_v2.0.a1 Prupe.6G158100_v2.0.a1 Prupe.8G025100_v2.0.a1 Prupe.8G042600_v2.0.a1
pyrus_communis pycom02g02250 pycom02g16030 pycom05g10490 pycom09g06450 pycom09g06460 pycom13g26200 pycom16g17700
rosa_chinensis RchiOBHm_Chr2g0163321 RchiOBHm_Chr4g0401981 RchiOBHm_Chr5g0005991 RchiOBHm_Chr5g0027191 RchiOBHm_Chr6g0249771
rosa_laevigata RLG00000001040 RLG00000001764 RLG00000002239 RLG00000002240 RLG00000003259 RLG00000008993 RLG00000013847 RLG00000014178 RLG00000015576 RLG00000017269 RLG00000017355 RLG00000018127 RLG00000018602 RLG00000018615 RLG00000019904 RLG00000020044 RLG00000021849 RLG00000021850 RLG00000027965 RLG00000029266 RLG00000029820 RLG00000031112 RLG00000031915 RLG00000033568 RLG00000035013 RLG00000035677 RLG00000036568 RLG00000036628
rosa_multiflora Rmu_co8254085.1_g000001 Rmu_co8462383.1_g000001 Rmu_sc0000185.1_g000049 Rmu_sc0000251.1_g000018 Rmu_sc0000367.1_g000094 Rmu_sc0000498.1_g000023 Rmu_sc0000868.1_g000005 Rmu_sc0001521.1_g000047 Rmu_sc0001534.1_g000007 Rmu_sc0002014.1_g000012 Rmu_sc0003214.1_g000009 Rmu_sc0003573.1_g000041 Rmu_sc0003832.1_g000023 Rmu_sc0004677.1_g000015 Rmu_sc0004967.1_g000014 Rmu_sc0005426.1_g000001 Rmu_sc0005621.1_g000004 Rmu_sc0005640.1_g000009 Rmu_sc0005947.1_g000034 Rmu_sc0006889.1_g000011 Rmu_sc0007121.1_g000014 Rmu_sc0009191.1_g000017 Rmu_sc0010556.1_g000011 Rmu_sc0011396.1_g000007 Rmu_sc0019862.1_g000002 Rmu_sc0022931.1_g000003 Rmu_sc0024164.1_g000001 Rmu_ssc0000391.1_g000027
rosa_roxburghii Rroxscaffold_1G00050060 Rroxscaffold_1G00061810 Rroxscaffold_2G00109590 Rroxscaffold_2G00113810 Rroxscaffold_2G00124040 Rroxscaffold_2G00143620 Rroxscaffold_2G00154760 Rroxscaffold_4G00302820 Rroxscaffold_4G00302830 Rroxscaffold_4G00308680 Rroxscaffold_4G00310240 Rroxscaffold_4G00327890 Rroxscaffold_5G00334600 Rroxscaffold_5G00336050 Rroxscaffold_5G00346560 Rroxscaffold_5G00347860 Rroxscaffold_5G00347870 Rroxscaffold_5G00349330 Rroxscaffold_5G00351980 Rroxscaffold_6G00395280 Rroxscaffold_6G00396750 Rroxscaffold_6G00402910 Rroxscaffold_7G00174790 Rroxscaffold_7G00177570 Rroxscaffold_7G00191820 Rroxscaffold_7G00203480 Rroxscaffold_7G00204380
rosa_rugosa Rorug01G0106600 Rorug01G0106600 Rorug01G0148500.1 Rorug02G0224200 Rorug02G0225500 Rorug02G0233000 Rorug02G0630400 Rorug03G0198000 Rorug03G0232600.1 Rorug03G0240500 Rorug04G0032900 Rorug04G0073300 Rorug04G0118500 Rorug04G0149600.1 Rorug04G0205100 Rorug04G0256700 Rorug05G0020200 Rorug05G0050000 Rorug05G0247300 Rorug05G0295100 Rorug05G0337900 Rorug05G0542600 Rorug06G0034800 Rorug06G0035100 Rorug06G0220100 Rorug07G0091900 Rorug07G0271200
rosa_samantha Rh1AG118200 Rh1AG253000 Rh1AG296300 Rh1AG409000 Rh1AG410600 Rh1BG073400 Rh1BG130400 Rh1BG147300 Rh1BG163600 Rh1CG016000 Rh1DG012200 Rh1DG012300 Rh1DG078600 Rh1DG078800 Rh1DG138100 Rh1DG179200 Rh1DG192600 Rh1DG194500 Rh1DG194600 Rh1DG214300 Rh1DG245900 Rh1DG267000 Rh1DG322700 Rh1DG442700 Rh2AG282000 Rh2AG423000 Rh2AG423100 Rh2AG460700 Rh2AG466200 Rh2AG513400 Rh2AG547000 Rh2BG277800 Rh2BG281200 Rh2BG298300 Rh2BG304300 Rh2BG433500 Rh2CG276000 Rh2CG282600 Rh2CG325500 Rh2CG393000 Rh2DG319200 Rh2DG443400 Rh3AG169300 Rh3AG303600 Rh3BG195000 Rh3BG282600 Rh3BG343400 Rh3BG343500 Rh3BG344100 Rh3CG111700 Rh3CG185700 Rh3CG259100 Rh3CG293000 Rh3CG317900 Rh3CG340900 Rh3CG341000 Rh3CG341100 Rh3CG354700 Rh3CG359200 Rh3CG368100 Rh3DG179900 Rh3DG288700 Rh3DG302600 Rh3DG316000 Rh3DG324500 Rh3DG324600 Rh3DG343200 Rh3DG343300 Rh3DG362400 Rh4AG071800 Rh4BG034000 Rh4BG044300 Rh4BG044400 Rh4BG069600 Rh4BG157400 Rh4CG007000 Rh4CG030400 Rh4CG037900 Rh4CG077700 Rh4CG115000 Rh4CG162400 Rh4CG162500 Rh4CG165500 Rh4CG165600 Rh4CG172900 Rh4CG239700 Rh4DG055900 Rh4DG173200 Rh5AG354900 Rh5AG511100 Rh5BG112600 Rh5BG541000 Rh5BG541100 Rh5BG549300 Rh5CG004400 Rh5CG315000 Rh5CG563800 Rh5CG572100 Rh5DG112200 Rh5DG191300 Rh5DG407600 Rh5DG407700 Rh5DG567500 Rh6AG002600 Rh6AG051400 Rh6AG199800 Rh6AG199900 Rh6AG206200 Rh6AG391000 Rh6AG391100 Rh6BG007500 Rh6BG053400 Rh6BG225900 Rh6BG227100 Rh6BG232400 Rh6CG091100 Rh6CG125400 Rh6CG186700 Rh6CG222700 Rh6CG222800 Rh6CG235500 Rh6CG247200 Rh6CG331200 Rh6CG465100 Rh6DG183500 Rh6DG213500 Rh6DG238800 Rh6DG263700 Rh7AG211800 Rh7AG441800 Rh7BG008600 Rh7BG253000 Rh7BG390000 Rh7CG189600 Rh7CG401500 Rh7CG424200 Rh7CG428700 Rh7CG479800 Rh7CG494300 Rh7DG177000 Rh7DG218700 Rh7DG254000 Rh7DG294800 Rh7DG407200 Rh7DG448800 Rh7DG461500
rosa_wichuraiana Rw0G012180 Rw1G005270 Rw1G012310 Rw1G016890 Rw1G021700 Rw2G014380 Rw2G027690 Rw3G016400 Rw3G028230 Rw3G029320 Rw4G002060 Rw4G002100 Rw4G003980 Rw4G014140 Rw4G014770 Rw4G014780 Rw4G015760 Rw4G021230 Rw5G000520 Rw5G035610 Rw6G001680 Rw6G017890 Rw6G027440 Rw6G034130 Rw6G034170 Rw7G012430 Rw7G033870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 742
Acc65I GGTACC 1 cut(s) 464
AccB1I GGYRCC 1 cut(s) 464
AccB7I CCANNNNNTGG 1 cut(s) 382
AccII CGCG 2 cut(s) 415, 528
AccIII TCCGGA 1 cut(s) 1333
AciI CCGC 6 cut(s) 178, 413, 526, 649, 731, 1043
AclI AACGTT 1 cut(s) 667
AclWI GGATC 8 cut(s) 169, 388, 401, 578, 589, 903, 1105, 1192
AcoI YGGCCR 2 cut(s) 54, 646
AcsI RAATTY 3 cut(s) 152, 484, 1144
AcuI CTGAAG 1 cut(s) 732
AcyI GRCGYC 2 cut(s) 739, 954
AfaI GTAC 3 cut(s) 244, 466, 905
AfiI CCNNNNNNNGG 5 cut(s) 382, 1011, 1123, 1124, 1135
AgsI TTSAA 2 cut(s) 866, 1228
AjnI CCWGG 4 cut(s) 51, 194, 302, 691
AjuI GAANNNNNNNTTGG 4 cut(s) 846, 878, 1230, 1262
AluBI AGCT 6 cut(s) 146, 382, 445, 698, 869, 1322
AluI AGCT 6 cut(s) 146, 382, 445, 698, 869, 1322
Alw26I GTCTC 3 cut(s) 413, 697, 787
AlwI GGATC 8 cut(s) 169, 388, 401, 578, 589, 903, 1105, 1192
AlwNI CAGNNNCTG 2 cut(s) 82, 448
Ama87I CYCGRG 2 cut(s) 189, 1011
Aor13HI TCCGGA 1 cut(s) 1333
AoxI GGCC 6 cut(s) 54, 341, 646, 751, 1126, 1150
ApeKI GCWGC 3 cut(s) 167, 445, 869
ApoI RAATTY 3 cut(s) 152, 484, 1144
ArsI GACNNNNNNTTYG 6 cut(s) 309, 341, 696, 728, 1280, 1312
Asp718I GGTACC 1 cut(s) 464
AsuC2I CCSGG 3 cut(s) 561, 1012, 1013
AsuHPI GGTGA 2 cut(s) 241, 933
AvaI CYCGRG 2 cut(s) 189, 1011
AvaII GGWCC 6 cut(s) 85, 299, 373, 687, 893, 1007
BaeI ACNNNNGTAYC 4 cut(s) 226, 259, 887, 920
BalI TGGCCA 1 cut(s) 56
BamHI GGATCC 1 cut(s) 393
BanI GGYRCC 1 cut(s) 464
BbvI GCAGC 3 cut(s) 154, 432, 856
BccI CCATC 2 cut(s) 758, 853
BceAI ACGGC 1 cut(s) 1066
BciT130I CCWGG 4 cut(s) 53, 196, 304, 693
BclI TGATCA 2 cut(s) 73, 112
BcnI CCSGG 3 cut(s) 561, 1012, 1013
BcoDI GTCTC 3 cut(s) 413, 697, 787
BfaI CTAG 3 cut(s) 354, 359, 735
BisI GCNGC 5 cut(s) 168, 446, 649, 732, 870
BlpI GCTNAGC 1 cut(s) 441
BlsI GCNGC 5 cut(s) 169, 447, 650, 733, 871
Bme1390I CCNGG 7 cut(s) 53, 196, 304, 561, 693, 1012, 1013
Bme18I GGWCC 6 cut(s) 85, 299, 373, 687, 893, 1007
BmeT110I CYCGRG 2 cut(s) 189, 1011
BmrFI CCNGG 7 cut(s) 53, 196, 304, 561, 693, 1012, 1013
BmrI ACTGGG 1 cut(s) 716
BmsI GCATC 3 cut(s) 189, 339, 340
BmuI ACTGGG 1 cut(s) 716
BplI GAGNNNNNCTC 2 cut(s) 610, 642
BpmI CTGGAG 1 cut(s) 714
Bpu1102I GCTNAGC 1 cut(s) 441
BpuMI CCSGG 3 cut(s) 561, 1012, 1013
BsaBI GATNNNNATC 1 cut(s) 179
BsaHI GRCGYC 2 cut(s) 739, 954
BsaI GGTCTC 1 cut(s) 413
BsaJI CCNNGG 7 cut(s) 302, 403, 683, 691, 1010, 1011, 1122
BsaWI WCCGGW 3 cut(s) 370, 1004, 1333
Bsc4I CCNNNNNNNGG 5 cut(s) 382, 1011, 1123, 1124, 1135
Bse118I RCCGGY 1 cut(s) 461
Bse1I ACTGG 3 cut(s) 280, 558, 711
Bse8I GATNNNNATC 1 cut(s) 179
BseAI TCCGGA 1 cut(s) 1333
BseBI CCWGG 4 cut(s) 53, 196, 304, 693
BseDI CCNNGG 7 cut(s) 302, 403, 683, 691, 1010, 1011, 1122
BseGI GGATG 3 cut(s) 180, 370, 723
BseJI GATNNNNATC 1 cut(s) 179
BseLI CCNNNNNNNGG 5 cut(s) 382, 1011, 1123, 1124, 1135
BseMII CTCAG 9 cut(s) 51, 192, 455, 667, 690, 918, 1172, 1195, 1307
BseNI ACTGG 3 cut(s) 280, 558, 711
BseRI GAGGAG 2 cut(s) 985, 988
BseXI GCAGC 3 cut(s) 154, 432, 856
BseYI CCCAGC 1 cut(s) 448
Bsh1236I CGCG 2 cut(s) 415, 528
BshFI GGCC 6 cut(s) 56, 343, 648, 753, 1128, 1152
BshNI GGYRCC 1 cut(s) 464
BsiHKCI CYCGRG 2 cut(s) 189, 1011
BsiSI CCGG 7 cut(s) 371, 462, 560, 1005, 1012, 1040, 1334
BslFI GGGAC 6 cut(s) 285, 330, 673, 919, 965, 993
BslI CCNNNNNNNGG 5 cut(s) 382, 1011, 1123, 1124, 1135
BsmAI GTCTC 3 cut(s) 413, 697, 787
BsmFI GGGAC 6 cut(s) 285, 330, 673, 919, 965, 993
BsnI GGCC 6 cut(s) 56, 343, 648, 753, 1128, 1152
Bso31I GGTCTC 1 cut(s) 413
BsoBI CYCGRG 2 cut(s) 189, 1011
Bsp13I TCCGGA 1 cut(s) 1333
Bsp1720I GCTNAGC 1 cut(s) 441
Bsp19I CCATGG 1 cut(s) 403
BspACI CCGC 6 cut(s) 178, 413, 526, 649, 731, 1043
BspANI GGCC 6 cut(s) 56, 343, 648, 753, 1128, 1152
BspCNI CTCAG 9 cut(s) 50, 193, 454, 666, 691, 919, 1171, 1194, 1306
BspEI TCCGGA 1 cut(s) 1333
BspFNI CGCG 2 cut(s) 415, 528
BspPI GGATC 8 cut(s) 169, 388, 401, 578, 589, 903, 1105, 1192
BspT107I GGYRCC 1 cut(s) 464
BspTNI GGTCTC 1 cut(s) 413
BsrFI RCCGGY 1 cut(s) 461
BsrI ACTGG 3 cut(s) 280, 558, 711
BssAI RCCGGY 1 cut(s) 461
BssECI CCNNGG 7 cut(s) 302, 403, 683, 691, 1010, 1011, 1122
BssNI GRCGYC 2 cut(s) 739, 954
BssT1I CCWWGG 2 cut(s) 403, 1122
Bst2UI CCWGG 4 cut(s) 53, 196, 304, 693
Bst4CI ACNGT 7 cut(s) 242, 310, 469, 496, 644, 828, 903
Bst6I CTCTTC 3 cut(s) 132, 635, 1006
BstACI GRCGYC 2 cut(s) 739, 954
BstC8I GCNNGC 1 cut(s) 384
BstDEI CTNAG 9 cut(s) 37, 201, 441, 653, 699, 927, 1158, 1181, 1293
BstDSI CCRYGG 1 cut(s) 403
BstF5I GGATG 3 cut(s) 180, 370, 723
BstFNI CGCG 2 cut(s) 415, 528
BstMAI GTCTC 3 cut(s) 413, 697, 787
BstMWI GCNNNNNNNGC 2 cut(s) 1087, 1328
BstNI CCWGG 4 cut(s) 53, 196, 304, 693
BstSCI CCNGG 7 cut(s) 51, 194, 302, 559, 691, 1010, 1011
BstUI CGCG 2 cut(s) 415, 528
BstV1I GCAGC 3 cut(s) 154, 432, 856
BstX2I RGATCY 2 cut(s) 393, 581
BstXI CCANNNNNNTGG 3 cut(s) 267, 404, 764
BstYI RGATCY 2 cut(s) 393, 581
BsuRI GGCC 6 cut(s) 56, 343, 648, 753, 1128, 1152
BtgI CCRYGG 1 cut(s) 403
BtsCI GGATG 3 cut(s) 180, 370, 723
BtsIMutI CAGTG 2 cut(s) 551, 1189
Cac8I GCNNGC 1 cut(s) 384
CaiI CAGNNNCTG 2 cut(s) 82, 448
Cfr10I RCCGGY 1 cut(s) 461
Cfr9I CCCGGG 1 cut(s) 1011
CseI GACGC 1 cut(s) 962
Csp6I GTAC 3 cut(s) 243, 465, 904
CviAII CATG 3 cut(s) 107, 404, 431
CviQI GTAC 3 cut(s) 243, 465, 904
DdeI CTNAG 9 cut(s) 37, 201, 441, 653, 699, 927, 1158, 1181, 1293
EaeI YGGCCR 2 cut(s) 54, 646
Eam1104I CTCTTC 3 cut(s) 132, 635, 1006
EarI CTCTTC 3 cut(s) 132, 635, 1006
EciI GGCGGA 1 cut(s) 1058
Eco130I CCWWGG 2 cut(s) 403, 1122
Eco31I GGTCTC 1 cut(s) 413
Eco47I GGWCC 6 cut(s) 85, 299, 373, 687, 893, 1007
Eco57I CTGAAG 1 cut(s) 732
Eco88I CYCGRG 2 cut(s) 189, 1011
EcoO109I RGGNCCY 1 cut(s) 752
EcoRI GAATTC 1 cut(s) 484
EcoRII CCWGG 4 cut(s) 51, 194, 302, 691
EcoT14I CCWWGG 2 cut(s) 403, 1122
ErhI CCWWGG 2 cut(s) 403, 1122
FaeI CATG 3 cut(s) 110, 407, 434
FaqI GGGAC 6 cut(s) 285, 330, 673, 919, 965, 993
FatI CATG 3 cut(s) 106, 403, 430
FauI CCCGC 1 cut(s) 420
FbaI TGATCA 2 cut(s) 73, 112
Fnu4HI GCNGC 5 cut(s) 168, 446, 649, 732, 870
FokI GGATG 3 cut(s) 167, 377, 730
Fsp4HI GCNGC 5 cut(s) 168, 446, 649, 732, 870
FspBI CTAG 3 cut(s) 354, 359, 735
GluI GCNGC 5 cut(s) 168, 446, 649, 732, 870
GsaI CCCAGC 1 cut(s) 452
GsuI CTGGAG 1 cut(s) 714
HaeIII GGCC 6 cut(s) 56, 343, 648, 753, 1128, 1152
HapII CCGG 7 cut(s) 371, 462, 560, 1005, 1012, 1040, 1334
HgaI GACGC 1 cut(s) 962
Hin1I GRCGYC 2 cut(s) 739, 954
Hin1II CATG 3 cut(s) 110, 407, 434
HindIII AAGCTT 1 cut(s) 1320
HinfI GANTC 4 cut(s) 427, 522, 874, 1310
HpaII CCGG 7 cut(s) 371, 462, 560, 1005, 1012, 1040, 1334
HphI GGTGA 2 cut(s) 241, 933
Hpy166II GTNNAC 4 cut(s) 376, 532, 665, 896
Hpy188I TCNGA 9 cut(s) 40, 141, 202, 423, 521, 879, 928, 1102, 1197
Hpy188III TCNNGA 7 cut(s) 83, 391, 568, 790, 810, 1316, 1334
Hpy8I GTNNAC 4 cut(s) 376, 532, 665, 896
HpyAV CCTTC 4 cut(s) 256, 869, 948, 1018
HpyCH4III ACNGT 7 cut(s) 242, 310, 469, 496, 644, 828, 903
HpyCH4IV ACGT 2 cut(s) 667, 739
HpyCH4V TGCA 4 cut(s) 330, 675, 1076, 1304
HpyF10VI GCNNNNNNNGC 2 cut(s) 1087, 1328
HpyF3I CTNAG 9 cut(s) 37, 201, 441, 653, 699, 927, 1158, 1181, 1293
HpySE526I ACGT 2 cut(s) 667, 739
Hsp92I GRCGYC 2 cut(s) 739, 954
Hsp92II CATG 3 cut(s) 110, 407, 434
Kpn2I TCCGGA 1 cut(s) 1333
KpnI GGTACC 1 cut(s) 468
Ksp22I TGATCA 2 cut(s) 73, 112
LmnI GCTCC 2 cut(s) 695, 1336
Lsp1109I GCAGC 3 cut(s) 154, 432, 856
LweI GCATC 3 cut(s) 189, 339, 340
MaeI CTAG 3 cut(s) 354, 359, 735
MaeII ACGT 2 cut(s) 667, 739
MaeIII GTNAC 1 cut(s) 233
MboII GAAGA 4 cut(s) 119, 622, 854, 993
MflI RGATCY 2 cut(s) 393, 581
MlsI TGGCCA 1 cut(s) 56
MluCI AATT 5 cut(s) 152, 324, 484, 1053, 1144
MluNI TGGCCA 1 cut(s) 56
MlyI GAGTC 3 cut(s) 421, 531, 883
MmeI TCCRAC 3 cut(s) 485, 513, 1001
Mox20I TGGCCA 1 cut(s) 56
MroI TCCGGA 1 cut(s) 1333
MscI TGGCCA 1 cut(s) 56
MseI TTAA 2 cut(s) 291, 1052
MslI CAYNNNNRTG 1 cut(s) 435
Msp20I TGGCCA 1 cut(s) 56
MspA1I CMGCKG 2 cut(s) 382, 445
MspI CCGG 7 cut(s) 371, 462, 560, 1005, 1012, 1040, 1334
MspR9I CCNGG 7 cut(s) 53, 196, 304, 561, 693, 1012, 1013
MvaI CCWGG 4 cut(s) 53, 196, 304, 693
MvnI CGCG 2 cut(s) 415, 528
MwoI GCNNNNNNNGC 2 cut(s) 1087, 1328
NciI CCSGG 3 cut(s) 561, 1012, 1013
NcoI CCATGG 1 cut(s) 403
NlaIII CATG 3 cut(s) 110, 407, 434
NmeAIII GCCGAG 1 cut(s) 111
NmuCI GTSAC 1 cut(s) 233
PaeR7I CTCGAG 1 cut(s) 189
PfeI GAWTC 1 cut(s) 1310
PflMI CCANNNNNTGG 1 cut(s) 382
PkrI GCNGC 5 cut(s) 169, 447, 650, 733, 871
PleI GAGTC 3 cut(s) 421, 530, 882
PpsI GAGTC 3 cut(s) 421, 530, 882
Psp1406I AACGTT 1 cut(s) 667
Psp6I CCWGG 4 cut(s) 51, 194, 302, 691
PspFI CCCAGC 1 cut(s) 448
PspGI CCWGG 4 cut(s) 51, 194, 302, 691
PspXI VCTCGAGB 1 cut(s) 189
PstNI CAGNNNCTG 2 cut(s) 82, 448
PsuI RGATCY 2 cut(s) 393, 581
PvuII CAGCTG 2 cut(s) 382, 445
RsaI GTAC 3 cut(s) 244, 466, 905
RsaNI GTAC 3 cut(s) 243, 465, 904
RseI CAYNNNNRTG 1 cut(s) 435
SaqAI TTAA 2 cut(s) 291, 1052
SatI GCNGC 5 cut(s) 168, 446, 649, 732, 870
SchI GAGTC 3 cut(s) 421, 531, 883
ScrFI CCNGG 7 cut(s) 53, 196, 304, 561, 693, 1012, 1013
SfaNI GCATC 3 cut(s) 189, 339, 340
Sfr274I CTCGAG 1 cut(s) 189
SinI GGWCC 6 cut(s) 85, 299, 373, 687, 893, 1007
SlaI CTCGAG 1 cut(s) 189
SmaI CCCGGG 1 cut(s) 1013
SmiMI CAYNNNNRTG 1 cut(s) 435
SmlI CTYRAG 1 cut(s) 189
SmoI CTYRAG 1 cut(s) 189
Sse9I AATT 5 cut(s) 152, 324, 484, 1053, 1144
SsiI CCGC 6 cut(s) 178, 413, 526, 649, 731, 1043
SspMI CTAG 3 cut(s) 354, 359, 735
StyD4I CCNGG 7 cut(s) 51, 194, 302, 559, 691, 1010, 1011
StyI CCWWGG 2 cut(s) 403, 1122
TaaI ACNGT 7 cut(s) 242, 310, 469, 496, 644, 828, 903
TaiI ACGT 2 cut(s) 670, 742
TaqI TCGA 3 cut(s) 190, 222, 811
TaqII GACCGA 1 cut(s) 675
TasI AATT 5 cut(s) 152, 324, 484, 1053, 1144
TauI GCSGC 2 cut(s) 651, 734
TfiI GAWTC 1 cut(s) 1310
Tru1I TTAA 2 cut(s) 291, 1052
Tru9I TTAA 2 cut(s) 291, 1052
TscAI CASTG 2 cut(s) 558, 1189
TseFI GTSAC 1 cut(s) 233
TseI GCWGC 3 cut(s) 167, 445, 869
Tsp45I GTSAC 1 cut(s) 233
TspDTI ATGAA 3 cut(s) 206, 1238, 1259
TspGWI ACGGA 1 cut(s) 221
TspMI CCCGGG 1 cut(s) 1011
TspRI CASTG 2 cut(s) 558, 1189
Van91I CCANNNNNTGG 1 cut(s) 382
VpaK11BI GGWCC 6 cut(s) 85, 299, 373, 687, 893, 1007
XapI RAATTY 3 cut(s) 152, 484, 1144
XcmI CCANNNNNNNNNTGG 1 cut(s) 259
XhoI CTCGAG 1 cut(s) 189
XmaI CCCGGG 1 cut(s) 1011
XspI CTAG 3 cut(s) 354, 359, 735
ZraI GACGTC 1 cut(s) 740
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.