Rw1G016890

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000073
Physical Location & Seq
Reverse (-)
37489774 .. 37491827
2054 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G016890.1

Sequence Viewer

Length: 642 bp
ATGAATTCCTTATCGTCATCTCAAAGGGAAATTCCCTATGAGGATGAAGGAATCATTGAGGCAATGTCAGTCTGCTTTGAGGACAATGCAGACTTCGTGGACATCGAAGAGGGAATCAGTCTCCTGGGTATCCTTATTGCTGATGAAGAGCCTGGGAAGACCCCTTCCATCACTGGTAAAAGGGGCTACCTTAGATTGAGAGTCGATTTTGATACGAGCAGACCTCTAGCAACTTTCATTCAACTGCCAAGAGCCAACTCCTCTTCATCCAGGATCCGCCTACAGTATGAGAACCTCCATAATTTCTGCTTCAACTGCGGCCGTCTAGGTCATATGGTCTCCTCATGCAGGTACCAAGTCAATCCTGTTCTATTATCGATGGGGGTCAAGTATGATAATTCCCTGATTGCTGCACCTCCACAAAAAGCAGTCTTTACTCAATCCAATTTTCCGGCTGAGTTCCCCTATGCCCCTACAACCGGTGTCTTTGGCCGGAACAAACTTGGGGGAAGGTTACCTCAAAATTCAAATTCTGGGGAAGCGGGATCTTCAGAGTACAATAGACCTACCAGGGCACACAATTCAACTTCTGTGGATGATCATGGTCTGGGCTTAATCATAGTGAGGGGGAGCCTTGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 RVT_3 zf-CCHC_4

Protein Analysis

213

Amino Acids

23.34

Weight (kDa)

5.82

Isoelectric Point (pI)

47.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CCHC_4 PF14392 70 - 118 4.2e-10 Zinc knuckle
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000073)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g17821 FvH4_2g04610 FvH4_2g09611 FvH4_2g13563 FvH4_3g21493 FvH4_3g32115 FvH4_3g35060 FvH4_4g00220 FvH4_4g06410 FvH4_4g10367 FvH4_4g10367 FvH4_4g10367 FvH4_5g06101 FvH4_5g08842 FvH4_5g27366 FvH4_5g31283 FvH4_6g11611 FvH4_6g23032 FvH4_7g05661 FvH4_7g06172
malus_domestica MD13G1263700.v1.1
prunus_persica Prupe.1G292300_v2.0.a1 Prupe.5G009700_v2.0.a1 Prupe.6G158100_v2.0.a1 Prupe.8G025100_v2.0.a1 Prupe.8G042600_v2.0.a1
pyrus_communis pycom02g02250 pycom02g16030 pycom05g10490 pycom09g06450 pycom09g06460 pycom13g26200 pycom16g17700
rosa_chinensis RchiOBHm_Chr2g0163321 RchiOBHm_Chr4g0401981 RchiOBHm_Chr5g0005991 RchiOBHm_Chr5g0027191 RchiOBHm_Chr6g0249771
rosa_laevigata RLG00000001040 RLG00000001764 RLG00000002239 RLG00000002240 RLG00000003259 RLG00000008993 RLG00000013847 RLG00000014178 RLG00000015576 RLG00000017269 RLG00000017355 RLG00000018127 RLG00000018602 RLG00000018615 RLG00000019904 RLG00000020044 RLG00000021849 RLG00000021850 RLG00000027965 RLG00000029266 RLG00000029820 RLG00000031112 RLG00000031915 RLG00000033568 RLG00000035013 RLG00000035677 RLG00000036568 RLG00000036628
rosa_multiflora Rmu_co8254085.1_g000001 Rmu_co8462383.1_g000001 Rmu_sc0000185.1_g000049 Rmu_sc0000251.1_g000018 Rmu_sc0000367.1_g000094 Rmu_sc0000498.1_g000023 Rmu_sc0000868.1_g000005 Rmu_sc0001521.1_g000047 Rmu_sc0001534.1_g000007 Rmu_sc0002014.1_g000012 Rmu_sc0003214.1_g000009 Rmu_sc0003573.1_g000041 Rmu_sc0003832.1_g000023 Rmu_sc0004677.1_g000015 Rmu_sc0004967.1_g000014 Rmu_sc0005426.1_g000001 Rmu_sc0005621.1_g000004 Rmu_sc0005640.1_g000009 Rmu_sc0005947.1_g000034 Rmu_sc0006889.1_g000011 Rmu_sc0007121.1_g000014 Rmu_sc0009191.1_g000017 Rmu_sc0010556.1_g000011 Rmu_sc0011396.1_g000007 Rmu_sc0019862.1_g000002 Rmu_sc0022931.1_g000003 Rmu_sc0024164.1_g000001 Rmu_ssc0000391.1_g000027
rosa_roxburghii Rroxscaffold_1G00050060 Rroxscaffold_1G00061810 Rroxscaffold_2G00109590 Rroxscaffold_2G00113810 Rroxscaffold_2G00124040 Rroxscaffold_2G00143620 Rroxscaffold_2G00154760 Rroxscaffold_4G00302820 Rroxscaffold_4G00302830 Rroxscaffold_4G00308680 Rroxscaffold_4G00310240 Rroxscaffold_4G00327890 Rroxscaffold_5G00334600 Rroxscaffold_5G00336050 Rroxscaffold_5G00346560 Rroxscaffold_5G00347860 Rroxscaffold_5G00347870 Rroxscaffold_5G00349330 Rroxscaffold_5G00351980 Rroxscaffold_6G00395280 Rroxscaffold_6G00396750 Rroxscaffold_6G00402910 Rroxscaffold_7G00174790 Rroxscaffold_7G00177570 Rroxscaffold_7G00191820 Rroxscaffold_7G00203480 Rroxscaffold_7G00204380
rosa_rugosa Rorug01G0106600 Rorug01G0106600 Rorug01G0148500.1 Rorug02G0224200 Rorug02G0225500 Rorug02G0233000 Rorug02G0630400 Rorug03G0198000 Rorug03G0232600.1 Rorug03G0240500 Rorug04G0032900 Rorug04G0073300 Rorug04G0118500 Rorug04G0149600.1 Rorug04G0205100 Rorug04G0256700 Rorug05G0020200 Rorug05G0050000 Rorug05G0247300 Rorug05G0295100 Rorug05G0337900 Rorug05G0542600 Rorug06G0034800 Rorug06G0035100 Rorug06G0220100 Rorug07G0091900 Rorug07G0271200
rosa_samantha Rh1AG118200 Rh1AG253000 Rh1AG296300 Rh1AG409000 Rh1AG410600 Rh1BG073400 Rh1BG130400 Rh1BG147300 Rh1BG163600 Rh1CG016000 Rh1DG012200 Rh1DG012300 Rh1DG078600 Rh1DG078800 Rh1DG138100 Rh1DG179200 Rh1DG192600 Rh1DG194500 Rh1DG194600 Rh1DG214300 Rh1DG245900 Rh1DG267000 Rh1DG322700 Rh1DG442700 Rh2AG282000 Rh2AG423000 Rh2AG423100 Rh2AG460700 Rh2AG466200 Rh2AG513400 Rh2AG547000 Rh2BG277800 Rh2BG281200 Rh2BG298300 Rh2BG304300 Rh2BG433500 Rh2CG276000 Rh2CG282600 Rh2CG325500 Rh2CG393000 Rh2DG319200 Rh2DG443400 Rh3AG169300 Rh3AG303600 Rh3BG195000 Rh3BG282600 Rh3BG343400 Rh3BG343500 Rh3BG344100 Rh3CG111700 Rh3CG185700 Rh3CG259100 Rh3CG293000 Rh3CG317900 Rh3CG340900 Rh3CG341000 Rh3CG341100 Rh3CG354700 Rh3CG359200 Rh3CG368100 Rh3DG179900 Rh3DG288700 Rh3DG302600 Rh3DG316000 Rh3DG324500 Rh3DG324600 Rh3DG343200 Rh3DG343300 Rh3DG362400 Rh4AG071800 Rh4BG034000 Rh4BG044300 Rh4BG044400 Rh4BG069600 Rh4BG157400 Rh4CG007000 Rh4CG030400 Rh4CG037900 Rh4CG077700 Rh4CG115000 Rh4CG162400 Rh4CG162500 Rh4CG165500 Rh4CG165600 Rh4CG172900 Rh4CG239700 Rh4DG055900 Rh4DG173200 Rh5AG354900 Rh5AG511100 Rh5BG112600 Rh5BG541000 Rh5BG541100 Rh5BG549300 Rh5CG004400 Rh5CG315000 Rh5CG563800 Rh5CG572100 Rh5DG112200 Rh5DG191300 Rh5DG407600 Rh5DG407700 Rh5DG567500 Rh6AG002600 Rh6AG051400 Rh6AG199800 Rh6AG199900 Rh6AG206200 Rh6AG391000 Rh6AG391100 Rh6BG007500 Rh6BG053400 Rh6BG225900 Rh6BG227100 Rh6BG232400 Rh6CG091100 Rh6CG125400 Rh6CG186700 Rh6CG222700 Rh6CG222800 Rh6CG235500 Rh6CG247200 Rh6CG331200 Rh6CG465100 Rh6DG183500 Rh6DG213500 Rh6DG238800 Rh6DG263700 Rh7AG211800 Rh7AG441800 Rh7BG008600 Rh7BG253000 Rh7BG390000 Rh7CG189600 Rh7CG401500 Rh7CG424200 Rh7CG428700 Rh7CG479800 Rh7CG494300 Rh7DG177000 Rh7DG218700 Rh7DG254000 Rh7DG294800 Rh7DG407200 Rh7DG448800 Rh7DG461500
rosa_wichuraiana Rw0G012180 Rw1G005270 Rw1G012310 Rw1G016890 Rw1G021700 Rw2G014380 Rw2G027690 Rw3G016400 Rw3G028230 Rw3G029320 Rw4G002060 Rw4G002100 Rw4G003980 Rw4G014140 Rw4G014770 Rw4G014780 Rw4G015760 Rw4G021230 Rw5G000520 Rw5G035610 Rw6G001680 Rw6G017890 Rw6G027440 Rw6G034130 Rw6G034170 Rw7G012430 Rw7G033870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 339
Acc65I GGTACC 1 cut(s) 351
AccB1I GGYRCC 1 cut(s) 351
AciI CCGC 3 cut(s) 277, 318, 542
AclWI GGATC 3 cut(s) 268, 281, 553
AcoI YGGCCR 2 cut(s) 319, 490
AcsI RAATTY 4 cut(s) 4, 30, 523, 529
AcuI CTGAAG 1 cut(s) 534
AfaI GTAC 2 cut(s) 353, 557
AfiI CCNNNNNNNGG 2 cut(s) 348, 479
AgeI ACCGGT 1 cut(s) 479
AgsI TTSAA 4 cut(s) 242, 313, 528, 585
AjnI CCWGG 4 cut(s) 123, 151, 269, 569
Alw26I GTCTC 2 cut(s) 125, 343
AlwI GGATC 3 cut(s) 268, 281, 553
AoxI GGCC 2 cut(s) 319, 490
ApeKI GCWGC 1 cut(s) 410
ApoI RAATTY 4 cut(s) 4, 30, 523, 529
AsiGI ACCGGT 1 cut(s) 479
Asp718I GGTACC 1 cut(s) 351
BaeGI GKGCMC 1 cut(s) 577
BamHI GGATCC 1 cut(s) 273
BanI GGYRCC 1 cut(s) 351
BbsI GAAGAC 1 cut(s) 164
BbvI GCAGC 1 cut(s) 397
BccI CCATC 2 cut(s) 176, 373
BceAI ACGGC 1 cut(s) 306
BciT130I CCWGG 4 cut(s) 125, 153, 271, 571
BciVI GTATCC 1 cut(s) 140
BclI TGATCA 1 cut(s) 598
BcoDI GTCTC 2 cut(s) 125, 343
BfaI CTAG 2 cut(s) 227, 326
BfmI CTRYAG 1 cut(s) 281
BfuAI ACCTGC 1 cut(s) 339
BfuI GTATCC 1 cut(s) 140
BisI GCNGC 2 cut(s) 319, 411
BlsI GCNGC 2 cut(s) 320, 412
Bme1390I CCNGG 4 cut(s) 125, 153, 271, 571
BmiI GGNNCC 3 cut(s) 275, 353, 632
BmrFI CCNGG 4 cut(s) 125, 153, 271, 571
BpiI GAAGAC 1 cut(s) 164
BplI GAGNNNNNCTC 2 cut(s) 208, 240
Bsa29I ATCGAT 1 cut(s) 377
BsaI GGTCTC 1 cut(s) 343
BsaJI CCNNGG 4 cut(s) 124, 152, 570, 634
BsaWI WCCGGW 1 cut(s) 479
BsaXI ACNNNNNCTCC 1 cut(s) 622
Bsc4I CCNNNNNNNGG 2 cut(s) 348, 479
Bse118I RCCGGY 1 cut(s) 479
Bse1I ACTGG 1 cut(s) 178
Bse3DI GCAATG 1 cut(s) 69
BseBI CCWGG 4 cut(s) 125, 153, 271, 571
BseCI ATCGAT 1 cut(s) 377
BseDI CCNNGG 4 cut(s) 124, 152, 570, 634
BseGI GGATG 3 cut(s) 49, 266, 601
BseLI CCNNNNNNNGG 2 cut(s) 348, 479
BseMI GCAATG 1 cut(s) 69
BseMII CTCAG 1 cut(s) 447
BseNI ACTGG 1 cut(s) 178
BseRI GAGGAG 2 cut(s) 250, 331
BseSI GKGCMC 1 cut(s) 577
BseX3I CGGCCG 1 cut(s) 319
BseXI GCAGC 1 cut(s) 397
BsgI GTGCAG 1 cut(s) 396
Bsh1285I CGRYCG 1 cut(s) 322
BshFI GGCC 2 cut(s) 321, 492
BshNI GGYRCC 1 cut(s) 351
BshTI ACCGGT 1 cut(s) 479
BshVI ATCGAT 1 cut(s) 377
BsiEI CGRYCG 1 cut(s) 322
BsiSI CCGG 3 cut(s) 452, 480, 493
BslI CCNNNNNNNGG 2 cut(s) 348, 479
BsmAI GTCTC 2 cut(s) 125, 343
BsnI GGCC 2 cut(s) 321, 492
Bso31I GGTCTC 1 cut(s) 343
Bsp1286I GDGCHC 1 cut(s) 577
Bsp143I GATC 3 cut(s) 273, 545, 598
BspACI CCGC 3 cut(s) 277, 318, 542
BspANI GGCC 2 cut(s) 321, 492
BspCNI CTCAG 1 cut(s) 448
BspDI ATCGAT 1 cut(s) 377
BspLI GGNNCC 3 cut(s) 275, 353, 632
BspMI ACCTGC 1 cut(s) 339
BspPI GGATC 3 cut(s) 268, 281, 553
BspQI GCTCTTC 1 cut(s) 141
BspT107I GGYRCC 1 cut(s) 351
BspTNI GGTCTC 1 cut(s) 343
BsrDI GCAATG 1 cut(s) 69
BsrFI RCCGGY 1 cut(s) 479
BsrI ACTGG 1 cut(s) 178
BssAI RCCGGY 1 cut(s) 479
BssECI CCNNGG 4 cut(s) 124, 152, 570, 634
BssMI GATC 3 cut(s) 273, 545, 598
BssT1I CCWWGG 1 cut(s) 634
Bst2UI CCWGG 4 cut(s) 125, 153, 271, 571
Bst4CI ACNGT 1 cut(s) 285
Bst6I CTCTTC 3 cut(s) 102, 141, 268
BstDEI CTNAG 2 cut(s) 191, 456
BstEII GGTNACC 1 cut(s) 513
BstENI CCTNNNNNAGG 1 cut(s) 346
BstF5I GGATG 3 cut(s) 49, 266, 601
BstKTI GATC 3 cut(s) 276, 548, 601
BstMAI GTCTC 2 cut(s) 125, 343
BstMBI GATC 3 cut(s) 273, 545, 598
BstMCI CGRYCG 1 cut(s) 322
BstMWI GCNNNNNNNGC 1 cut(s) 315
BstNI CCWGG 4 cut(s) 125, 153, 271, 571
BstPI GGTNACC 1 cut(s) 513
BstSCI CCNGG 4 cut(s) 123, 151, 269, 569
BstSFI CTRYAG 1 cut(s) 281
BstSLI GKGCMC 1 cut(s) 577
BstV1I GCAGC 1 cut(s) 397
BstV2I GAAGAC 1 cut(s) 164
BstX2I RGATCY 2 cut(s) 273, 545
BstYI RGATCY 2 cut(s) 273, 545
BstZI CGGCCG 1 cut(s) 319
Bsu15I ATCGAT 1 cut(s) 377
BsuI GTATCC 1 cut(s) 140
BsuRI GGCC 2 cut(s) 321, 492
BsuTUI ATCGAT 1 cut(s) 377
BtsCI GGATG 3 cut(s) 49, 266, 601
BtsIMutI CAGTG 1 cut(s) 171
BveI ACCTGC 1 cut(s) 339
Cfr10I RCCGGY 1 cut(s) 479
ClaI ATCGAT 1 cut(s) 377
Csp6I GTAC 2 cut(s) 352, 556
CspAI ACCGGT 1 cut(s) 479
CspCI CAANNNNNGTGG 2 cut(s) 573, 608
CviAII CATG 2 cut(s) 345, 602
CviJI RGCY 8 cut(s) 151, 186, 254, 321, 455, 492, 612, 633
CviKI_1 RGCY 8 cut(s) 151, 186, 254, 321, 455, 492, 612, 633
CviQI GTAC 2 cut(s) 352, 556
DdeI CTNAG 2 cut(s) 191, 456
DpnI GATC 3 cut(s) 275, 547, 600
DpnII GATC 3 cut(s) 273, 545, 598
EaeI YGGCCR 2 cut(s) 319, 490
EagI CGGCCG 1 cut(s) 319
Eam1104I CTCTTC 3 cut(s) 102, 141, 268
EarI CTCTTC 3 cut(s) 102, 141, 268
EciI GGCGGA 1 cut(s) 266
EclXI CGGCCG 1 cut(s) 319
Eco130I CCWWGG 1 cut(s) 634
Eco31I GGTCTC 1 cut(s) 343
Eco52I CGGCCG 1 cut(s) 319
Eco57I CTGAAG 1 cut(s) 534
Eco91I GGTNACC 1 cut(s) 513
EcoNI CCTNNNNNAGG 1 cut(s) 346
EcoO65I GGTNACC 1 cut(s) 513
EcoRI GAATTC 1 cut(s) 4
EcoRII CCWGG 4 cut(s) 123, 151, 269, 569
EcoT14I CCWWGG 1 cut(s) 634
ErhI CCWWGG 1 cut(s) 634
FaeI CATG 2 cut(s) 348, 605
FatI CATG 2 cut(s) 344, 601
FauI CCCGC 1 cut(s) 535
FauNDI CATATG 1 cut(s) 333
FbaI TGATCA 1 cut(s) 598
Fnu4HI GCNGC 2 cut(s) 319, 411
FokI GGATG 3 cut(s) 56, 253, 608
Fsp4HI GCNGC 2 cut(s) 319, 411
FspBI CTAG 2 cut(s) 227, 326
GluI GCNGC 2 cut(s) 319, 411
HaeIII GGCC 2 cut(s) 321, 492
HapII CCGG 3 cut(s) 452, 480, 493
Hin1II CATG 2 cut(s) 348, 605
HinfI GANTC 3 cut(s) 51, 114, 201
HpaII CCGG 3 cut(s) 452, 480, 493
Hpy166II GTNNAC 1 cut(s) 100
Hpy188I TCNGA 1 cut(s) 553
Hpy8I GTNNAC 1 cut(s) 100
HpyAV CCTTC 3 cut(s) 41, 174, 504
HpyCH4III ACNGT 1 cut(s) 285
HpyCH4V TGCA 3 cut(s) 89, 348, 413
HpyF10VI GCNNNNNNNGC 1 cut(s) 315
HpyF3I CTNAG 2 cut(s) 191, 456
Hsp92II CATG 2 cut(s) 348, 605
KpnI GGTACC 1 cut(s) 355
Ksp22I TGATCA 1 cut(s) 598
Kzo9I GATC 3 cut(s) 273, 545, 598
LguI GCTCTTC 1 cut(s) 141
LmnI GCTCC 1 cut(s) 630
Lsp1109I GCAGC 1 cut(s) 397
MaeI CTAG 2 cut(s) 227, 326
MaeIII GTNAC 1 cut(s) 513
MalI GATC 3 cut(s) 275, 547, 600
MboI GATC 3 cut(s) 273, 545, 598
MboII GAAGA 5 cut(s) 119, 158, 169, 255, 540
MflI RGATCY 2 cut(s) 273, 545
MhlI GDGCHC 1 cut(s) 577
MluCI AATT 8 cut(s) 4, 30, 301, 397, 445, 523, 529, 580
MlyI GAGTC 1 cut(s) 210
MseI TTAA 1 cut(s) 614
MspI CCGG 3 cut(s) 452, 480, 493
MspR9I CCNGG 4 cut(s) 125, 153, 271, 571
MvaI CCWGG 4 cut(s) 125, 153, 271, 571
MwoI GCNNNNNNNGC 1 cut(s) 315
NdeI CATATG 1 cut(s) 333
NdeII GATC 3 cut(s) 273, 545, 598
NlaIII CATG 2 cut(s) 348, 605
NlaIV GGNNCC 3 cut(s) 275, 353, 632
PciSI GCTCTTC 1 cut(s) 141
PcsI WCGNNNNNNNCGW 1 cut(s) 102
PfeI GAWTC 2 cut(s) 51, 114
PfoI TCCNGGA 1 cut(s) 269
PinAI ACCGGT 1 cut(s) 479
PkrI GCNGC 2 cut(s) 320, 412
PleI GAGTC 1 cut(s) 209
PpsI GAGTC 1 cut(s) 209
Psp6I CCWGG 4 cut(s) 123, 151, 269, 569
PspEI GGTNACC 1 cut(s) 513
PspGI CCWGG 4 cut(s) 123, 151, 269, 569
PspN4I GGNNCC 3 cut(s) 275, 353, 632
PsuI RGATCY 2 cut(s) 273, 545
RsaI GTAC 2 cut(s) 353, 557
RsaNI GTAC 2 cut(s) 352, 556
SapI GCTCTTC 1 cut(s) 141
SaqAI TTAA 1 cut(s) 614
SatI GCNGC 2 cut(s) 319, 411
Sau3AI GATC 3 cut(s) 273, 545, 598
SchI GAGTC 1 cut(s) 210
ScrFI CCNGG 4 cut(s) 125, 153, 271, 571
SduI GDGCHC 1 cut(s) 577
SetI ASST 9 cut(s) 192, 226, 297, 331, 353, 418, 515, 520, 568
SfcI CTRYAG 1 cut(s) 281
Sse9I AATT 8 cut(s) 4, 30, 301, 397, 445, 523, 529, 580
SsiI CCGC 3 cut(s) 277, 318, 542
SspMI CTAG 2 cut(s) 227, 326
StyD4I CCNGG 4 cut(s) 123, 151, 269, 569
StyI CCWWGG 1 cut(s) 634
TaaI ACNGT 1 cut(s) 285
TaqI TCGA 3 cut(s) 105, 204, 377
TasI AATT 8 cut(s) 4, 30, 301, 397, 445, 523, 529, 580
TatI WGTACW 1 cut(s) 555
TauI GCSGC 1 cut(s) 321
TfiI GAWTC 2 cut(s) 51, 114
Tru1I TTAA 1 cut(s) 614
Tru9I TTAA 1 cut(s) 614
TscAI CASTG 1 cut(s) 178
TseI GCWGC 1 cut(s) 410
TspDTI ATGAA 5 cut(s) 17, 60, 159, 226, 255
TspRI CASTG 1 cut(s) 178
XagI CCTNNNNNAGG 1 cut(s) 346
XapI RAATTY 4 cut(s) 4, 30, 523, 529
XspI CTAG 2 cut(s) 227, 326
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.