Domain of unknown function (DUF4283)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 602 |
| AccI | GTMKAC | 1 cut(s) | 502 |
| AccII | CGCG | 1 cut(s) | 569 |
| AciI | CCGC | 3 cut(s) | 536, 567, 569 |
| AclWI | GGATC | 2 cut(s) | 26, 62 |
| AcoI | YGGCCR | 1 cut(s) | 649 |
| AcsI | RAATTY | 2 cut(s) | 618, 655 |
| AfaI | GTAC | 1 cut(s) | 279 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 15, 239, 737 |
| AgsI | TTSAA | 5 cut(s) | 107, 353, 524, 626, 716 |
| AjnI | CCWGG | 1 cut(s) | 708 |
| AluBI | AGCT | 6 cut(s) | 53, 101, 370, 477, 494, 720 |
| AluI | AGCT | 6 cut(s) | 53, 101, 370, 477, 494, 720 |
| Alw21I | GWGCWC | 1 cut(s) | 676 |
| Alw26I | GTCTC | 1 cut(s) | 566 |
| AlwI | GGATC | 2 cut(s) | 26, 62 |
| AoxI | GGCC | 2 cut(s) | 86, 649 |
| ApeKI | GCWGC | 1 cut(s) | 696 |
| ApoI | RAATTY | 2 cut(s) | 618, 655 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 111 |
| AsuHPI | GGTGA | 2 cut(s) | 221, 257 |
| BanII | GRGCYC | 2 cut(s) | 427, 743 |
| BauI | CACGAG | 1 cut(s) | 54 |
| Bbv12I | GWGCWC | 1 cut(s) | 676 |
| BbvI | GCAGC | 1 cut(s) | 708 |
| BccI | CCATC | 3 cut(s) | 242, 281, 326 |
| BciT130I | CCWGG | 1 cut(s) | 710 |
| BcoDI | GTCTC | 1 cut(s) | 566 |
| BfaI | CTAG | 3 cut(s) | 324, 440, 597 |
| BfmI | CTRYAG | 1 cut(s) | 256 |
| BfuAI | ACCTGC | 1 cut(s) | 602 |
| BisI | GCNGC | 2 cut(s) | 567, 697 |
| BlsI | GCNGC | 2 cut(s) | 568, 698 |
| Bme1390I | CCNGG | 1 cut(s) | 710 |
| BmiI | GGNNCC | 1 cut(s) | 253 |
| BmrFI | CCNGG | 1 cut(s) | 710 |
| BmsI | GCATC | 1 cut(s) | 129 |
| BpuEI | CTTGAG | 1 cut(s) | 651 |
| BsaI | GGTCTC | 1 cut(s) | 566 |
| BsaJI | CCNNGG | 1 cut(s) | 567 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 15, 239, 737 |
| Bse1I | ACTGG | 2 cut(s) | 506, 660 |
| BseBI | CCWGG | 1 cut(s) | 710 |
| BseDI | CCNNGG | 1 cut(s) | 567 |
| BseGI | GGATG | 3 cut(s) | 144, 232, 486 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 15, 239, 737 |
| BseNI | ACTGG | 2 cut(s) | 506, 660 |
| BseXI | GCAGC | 1 cut(s) | 708 |
| BsgI | GTGCAG | 1 cut(s) | 563 |
| Bsh1236I | CGCG | 1 cut(s) | 569 |
| BshFI | GGCC | 2 cut(s) | 88, 651 |
| BsiHKAI | GWGCWC | 1 cut(s) | 676 |
| BsiSI | CCGG | 1 cut(s) | 581 |
| BslFI | GGGAC | 1 cut(s) | 655 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 15, 239, 737 |
| BsmAI | GTCTC | 1 cut(s) | 566 |
| BsmFI | GGGAC | 1 cut(s) | 655 |
| BsnI | GGCC | 2 cut(s) | 88, 651 |
| Bso31I | GGTCTC | 1 cut(s) | 566 |
| Bsp1286I | GDGCHC | 3 cut(s) | 427, 676, 743 |
| Bsp143I | GATC | 2 cut(s) | 18, 67 |
| BspACI | CCGC | 3 cut(s) | 536, 567, 569 |
| BspANI | GGCC | 2 cut(s) | 88, 651 |
| BspFNI | CGCG | 1 cut(s) | 569 |
| BspHI | TCATGA | 2 cut(s) | 216, 800 |
| BspLI | GGNNCC | 1 cut(s) | 253 |
| BspMI | ACCTGC | 1 cut(s) | 602 |
| BspPI | GGATC | 2 cut(s) | 26, 62 |
| BspTNI | GGTCTC | 1 cut(s) | 566 |
| BsrI | ACTGG | 2 cut(s) | 506, 660 |
| BssECI | CCNNGG | 1 cut(s) | 567 |
| BssMI | GATC | 2 cut(s) | 18, 67 |
| BssSI | CACGAG | 1 cut(s) | 54 |
| Bst2BI | CACGAG | 1 cut(s) | 54 |
| Bst2UI | CCWGG | 1 cut(s) | 710 |
| Bst4CI | ACNGT | 4 cut(s) | 260, 282, 392, 646 |
| BstC8I | GCNNGC | 4 cut(s) | 90, 306, 321, 372 |
| BstDEI | CTNAG | 1 cut(s) | 266 |
| BstDSI | CCRYGG | 1 cut(s) | 567 |
| BstEII | GGTNACC | 1 cut(s) | 196 |
| BstF5I | GGATG | 3 cut(s) | 144, 232, 486 |
| BstFNI | CGCG | 1 cut(s) | 569 |
| BstKTI | GATC | 2 cut(s) | 21, 70 |
| BstMAI | GTCTC | 1 cut(s) | 566 |
| BstMBI | GATC | 2 cut(s) | 18, 67 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 98, 563 |
| BstNI | CCWGG | 1 cut(s) | 710 |
| BstNSI | RCATGY | 1 cut(s) | 536 |
| BstPI | GGTNACC | 1 cut(s) | 196 |
| BstSCI | CCNGG | 1 cut(s) | 708 |
| BstSFI | CTRYAG | 1 cut(s) | 256 |
| BstUI | CGCG | 1 cut(s) | 569 |
| BstV1I | GCAGC | 1 cut(s) | 708 |
| BstX2I | RGATCY | 2 cut(s) | 18, 67 |
| BstYI | RGATCY | 2 cut(s) | 18, 67 |
| BsuRI | GGCC | 2 cut(s) | 88, 651 |
| BtgI | CCRYGG | 1 cut(s) | 567 |
| BtsCI | GGATG | 3 cut(s) | 144, 232, 486 |
| BtsIMutI | CAGTG | 1 cut(s) | 388 |
| BveI | ACCTGC | 1 cut(s) | 602 |
| Cac8I | GCNNGC | 4 cut(s) | 90, 306, 321, 372 |
| CciI | TCATGA | 2 cut(s) | 216, 800 |
| Cfr42I | CCGCGG | 1 cut(s) | 570 |
| CsiI | ACCWGGT | 1 cut(s) | 708 |
| Csp6I | GTAC | 1 cut(s) | 278 |
| CviAII | CATG | 3 cut(s) | 217, 533, 801 |
| CviQI | GTAC | 1 cut(s) | 278 |
| DdeI | CTNAG | 1 cut(s) | 266 |
| DpnI | GATC | 2 cut(s) | 20, 69 |
| DpnII | GATC | 2 cut(s) | 18, 67 |
| EaeI | YGGCCR | 1 cut(s) | 649 |
| Eco24I | GRGCYC | 2 cut(s) | 427, 743 |
| Eco31I | GGTCTC | 1 cut(s) | 566 |
| Eco91I | GGTNACC | 1 cut(s) | 196 |
| EcoO65I | GGTNACC | 1 cut(s) | 196 |
| EcoRII | CCWGG | 1 cut(s) | 708 |
| EcoT38I | GRGCYC | 2 cut(s) | 427, 743 |
| FaeI | CATG | 3 cut(s) | 220, 536, 804 |
| FaqI | GGGAC | 1 cut(s) | 655 |
| FatI | CATG | 3 cut(s) | 216, 532, 800 |
| FblI | GTMKAC | 1 cut(s) | 502 |
| Fnu4HI | GCNGC | 2 cut(s) | 567, 697 |
| FokI | GGATG | 3 cut(s) | 151, 239, 493 |
| FriOI | GRGCYC | 2 cut(s) | 427, 743 |
| Fsp4HI | GCNGC | 2 cut(s) | 567, 697 |
| FspBI | CTAG | 3 cut(s) | 324, 440, 597 |
| GluI | GCNGC | 2 cut(s) | 567, 697 |
| HaeIII | GGCC | 2 cut(s) | 88, 651 |
| HapII | CCGG | 1 cut(s) | 581 |
| Hin1II | CATG | 3 cut(s) | 220, 536, 804 |
| HincII | GTYRAC | 2 cut(s) | 486, 503 |
| HindII | GTYRAC | 2 cut(s) | 486, 503 |
| HindIII | AAGCTT | 1 cut(s) | 368 |
| HinfI | GANTC | 4 cut(s) | 176, 270, 446, 748 |
| HpaII | CCGG | 1 cut(s) | 581 |
| HphI | GGTGA | 2 cut(s) | 221, 257 |
| Hpy166II | GTNNAC | 3 cut(s) | 278, 486, 503 |
| Hpy188I | TCNGA | 2 cut(s) | 117, 422 |
| Hpy188III | TCNNGA | 5 cut(s) | 56, 71, 217, 597, 801 |
| Hpy8I | GTNNAC | 3 cut(s) | 278, 486, 503 |
| Hpy99I | CGWCG | 1 cut(s) | 504 |
| HpyAV | CCTTC | 1 cut(s) | 600 |
| HpyCH4III | ACNGT | 4 cut(s) | 260, 282, 392, 646 |
| HpyCH4V | TGCA | 4 cut(s) | 92, 142, 374, 544 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 98, 563 |
| HpyF3I | CTNAG | 1 cut(s) | 266 |
| Hsp92II | CATG | 3 cut(s) | 220, 536, 804 |
| KspI | CCGCGG | 1 cut(s) | 570 |
| Kzo9I | GATC | 2 cut(s) | 18, 67 |
| Lsp1109I | GCAGC | 1 cut(s) | 708 |
| LweI | GCATC | 1 cut(s) | 129 |
| MabI | ACCWGGT | 1 cut(s) | 708 |
| MaeI | CTAG | 3 cut(s) | 324, 440, 597 |
| MaeIII | GTNAC | 2 cut(s) | 24, 196 |
| MalI | GATC | 2 cut(s) | 20, 69 |
| MboI | GATC | 2 cut(s) | 18, 67 |
| MboII | GAAGA | 2 cut(s) | 86, 119 |
| MflI | RGATCY | 2 cut(s) | 18, 67 |
| MhlI | GDGCHC | 3 cut(s) | 427, 676, 743 |
| MluCI | AATT | 3 cut(s) | 519, 618, 655 |
| MlyI | GAGTC | 2 cut(s) | 185, 279 |
| MmeI | TCCRAC | 1 cut(s) | 140 |
| MnlI | CCTC | 8 cut(s) | 20, 143, 168, 245, 429, 537, 712, 730 |
| MroXI | GAANNNNTTC | 1 cut(s) | 111 |
| MseI | TTAA | 2 cut(s) | 42, 401 |
| MslI | CAYNNNNRTG | 2 cut(s) | 207, 221 |
| MspA1I | CMGCKG | 1 cut(s) | 569 |
| MspI | CCGG | 1 cut(s) | 581 |
| MspR9I | CCNGG | 1 cut(s) | 710 |
| MvaI | CCWGG | 1 cut(s) | 710 |
| MvnI | CGCG | 1 cut(s) | 569 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 98, 563 |
| NdeII | GATC | 2 cut(s) | 18, 67 |
| NlaIII | CATG | 3 cut(s) | 220, 536, 804 |
| NlaIV | GGNNCC | 1 cut(s) | 253 |
| NmuCI | GTSAC | 1 cut(s) | 24 |
| NspI | RCATGY | 1 cut(s) | 536 |
| PagI | TCATGA | 2 cut(s) | 216, 800 |
| PdmI | GAANNNNTTC | 1 cut(s) | 111 |
| PfeI | GAWTC | 2 cut(s) | 446, 748 |
| PkrI | GCNGC | 2 cut(s) | 568, 698 |
| PleI | GAGTC | 2 cut(s) | 184, 278 |
| PpsI | GAGTC | 2 cut(s) | 184, 278 |
| Psp6I | CCWGG | 1 cut(s) | 708 |
| PspEI | GGTNACC | 1 cut(s) | 196 |
| PspGI | CCWGG | 1 cut(s) | 708 |
| PspN4I | GGNNCC | 1 cut(s) | 253 |
| PsuI | RGATCY | 2 cut(s) | 18, 67 |
| RsaI | GTAC | 1 cut(s) | 279 |
| RsaNI | GTAC | 1 cut(s) | 278 |
| RseI | CAYNNNNRTG | 2 cut(s) | 207, 221 |
| SacII | CCGCGG | 1 cut(s) | 570 |
| SalI | GTCGAC | 1 cut(s) | 501 |
| SaqAI | TTAA | 2 cut(s) | 42, 401 |
| SatI | GCNGC | 2 cut(s) | 567, 697 |
| Sau3AI | GATC | 2 cut(s) | 18, 67 |
| SchI | GAGTC | 2 cut(s) | 185, 279 |
| ScrFI | CCNGG | 1 cut(s) | 710 |
| SduI | GDGCHC | 3 cut(s) | 427, 676, 743 |
| SexAI | ACCWGGT | 1 cut(s) | 708 |
| SfaNI | GCATC | 1 cut(s) | 129 |
| SfcI | CTRYAG | 1 cut(s) | 256 |
| Sfr303I | CCGCGG | 1 cut(s) | 570 |
| SgrBI | CCGCGG | 1 cut(s) | 570 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 207, 221 |
| SmlI | CTYRAG | 1 cut(s) | 666 |
| SmoI | CTYRAG | 1 cut(s) | 666 |
| Sse9I | AATT | 3 cut(s) | 519, 618, 655 |
| SsiI | CCGC | 3 cut(s) | 536, 567, 569 |
| SspMI | CTAG | 3 cut(s) | 324, 440, 597 |
| StyD4I | CCNGG | 1 cut(s) | 708 |
| TaaI | ACNGT | 4 cut(s) | 260, 282, 392, 646 |
| TaqI | TCGA | 2 cut(s) | 285, 502 |
| TasI | AATT | 3 cut(s) | 519, 618, 655 |
| TatI | WGTACW | 1 cut(s) | 277 |
| TauI | GCSGC | 1 cut(s) | 569 |
| TfiI | GAWTC | 2 cut(s) | 446, 748 |
| Tru1I | TTAA | 2 cut(s) | 42, 401 |
| Tru9I | TTAA | 2 cut(s) | 42, 401 |
| TscAI | CASTG | 1 cut(s) | 395 |
| TseFI | GTSAC | 1 cut(s) | 24 |
| TseI | GCWGC | 1 cut(s) | 696 |
| Tsp45I | GTSAC | 1 cut(s) | 24 |
| TspDTI | ATGAA | 3 cut(s) | 205, 378, 471 |
| TspGWI | ACGGA | 3 cut(s) | 31, 263, 616 |
| TspRI | CASTG | 1 cut(s) | 395 |
| XapI | RAATTY | 2 cut(s) | 618, 655 |
| XbaI | TCTAGA | 1 cut(s) | 596 |
| XceI | RCATGY | 1 cut(s) | 536 |
| XmiI | GTMKAC | 1 cut(s) | 502 |
| XmnI | GAANNNNTTC | 1 cut(s) | 111 |
| XspI | CTAG | 3 cut(s) | 324, 440, 597 |