FvH4_6g23032

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000073
Physical Location & Seq
Forward (+)
16786529 .. 16791209
4681 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g23032.t1

Sequence Viewer

Length: 2736 bp
ATGAGATGGGCCTTTCACCAAACAGGTCAGATACAGAGTACTATTATTAGTAATTCTATCCCCTCTTGTATTGCTTTGGAAACTCCTATTAAAGGTAATGTGACGGGGGTTATACAATTGGGTGATTCTGAGTTGGTTGTTGGGCCCTCGGCCCATGCTACTTCTCATCTTTTATTGGATCAACAGCCTGTTATTGAAAAAGGATTTTGTGGGTATCGACTTGAATCACTCGACACAGGAAGAGTCTTGGTGTTTGAACACTTCAAAGGTAACGGAGGATTCTACCACCCTCCGCGGCTGTGGCGGCTGGCCCGGAACAGCTGCAGGCAATCAATGAGGACAATTTCTTGGAACTGTCATGGTCTAGGCCGCACTGCGACAGTCAAAGCGATCTCTCGTCAAAGACCCAACATTTTATTTTTGTCTGAAACGCATAAAACTACTGGTTATGTGGAGAAGATCCGTAGGAAAATGAGATTCAGCCAGGGCTTCTGTGTTGACCCTATTGGGATTGCTGGAGGTCTTTGCCTATGGTGGGATGATTCACTGCTGATTAATATTGTCGACTTCTCTAAAAATTTCTTAGATACGATGATCACTTTGATTGAAACAAGAGAGGTGGTCCGGGCTACTTTTATTTATGGTCCCCCTTATCAGGAGGACAAGGAGGATTTTTGGAATGAATGGTCTTTTAGGGACAAAGGGGACTCGACTCTATGGTTGGTGATAGGGGATGTGAATGAATTACTCTCTCAGCAAGAACGTATTGGAGATGCAAATTGGGACTACCACAGACGTCGCTTTCTGGGTAGTTTTATAAACTCAAACAACCTTTTGGATGTAGGCTTTTCTGGACCTGCGTTTACTTGGACTAGGAAGGCATTTGGAAGGATTATTCTTCAAGAGCGTTTAGATAGAGCACTTGTATCAGCTGAGTGGCTGGTTTCGTGGCCAAATTCTTTAGTTATTCACTTGGTCAGAGTAGGATCTGACCATTGTCCTATACTTTTCTCGTTGGGCCTTAAGGAGAATAGAATTGGGCCTGGTTTTAAGTTTGAGTCCTATTGGGTTGATAAGGTGGAGATTGTGCCTATCGTAGGGCCTGTCAAGGAGGAAATCAAAGATGTTCTGCACGAACTTGAGGAAATTCAGTGTTCTGATCCTCTTGCTAATTGTGCCGGGGATTCCAATACCCGATTCTTCCATCTCTCTACACTAGAGAGGAGATGTCGAAACCGGATTCTTCGCATCCAGGATGGTGACGGTGTTTGGGTTTCTGGAGAGTACAAGATCAGAAAGGCATTTGAGAAGCAATTTATGGCTATTTTCACATCGGATGGCCCAAGGTATTGGGGGAACGTGATGGAGGGGGTTAGGAGTGTGATTACCGAGGAGATTAATCAAGATTTGATGGCTCCGGTGTTGGAAGAGGAAGTCAGAGCTGATGTCTTCCAGTTGTGTGCCCTCAAGGCACCCGGTCCGGATGGATTTCCAGGTTTATTCTATCATAAGTACTGGTGTATGGTTAAAGACATTATCACTAGGACTACTTCTGATTTCCTTGAAGGCAGTGTTTGTTTGAGGAAGATTAACAAGACACATATTGTTCTTATTCCTAAAGTGGAGGATAATATTTTGCTTGCCCATGAGACTTATCACTATCTCAAGTTGAAGCGGGAGGGTGGAAAACATGAGTTGGCACTCAAACTAGATATGGTAAAGGCCTATGATCGTGTTGAGTGGGACTTTTTGGAAGCGGCACTTATCCGATTTGGCTTCTCGAGGGATTGGATTCGCTTGATTATGGCTTGTGTCACTACTGTCTCTTTTGCTATTGTACTCAATGGGAAGCCAGGACGGACTTTTTACCCAACCCGGGGTCTTCGGAAGGGTGATCCCATATCCCCTTATTTGTTTCTATTGGTAAGTGAGGTTCTCTCACTCTGCTTGACTAAGTGTGTTGGGGATGGTAGCTTGATTGGGATTTGGTTGAGTAGAGGGGGCCTTATCATGTCCCATCTCTTCTTTGCGGATGATGCTCTCTTTTTTATCCGGGCCACCCTTGGAAATGCCTCGCGTCTTTCCCTAATTTTCAAGGATTACTGTACTGCTTCGGGTCAAGCTATTAGTCAGGAAAAATCAAAAAAGAAGGCACTTGGGTATATCAGCGACCGAATCAAGAGGAAGATGAGTGGGTGGAAACAAAATTCTCTTTCTTGGGCAGGAAAGGAGACTCTTATTAAGTCGGTAGCACTTCAATATGGCACTCTTGGCAAAATAGGTGTGGAGACTTGTGGAGAACCCTACCTCTTTATGGGCAGAGGTCCTAAAGTCTCGGTAGTTAATGGGTCCTCTGTTAGAGTATGGCAGGATAAATGGTTAACTAGTCCTTCTCTTAGACTTCTGCCTTCTTCTACTGTGCCGCGACCAACTGCTGTTGTGTTGGTTAATCAGCTAATTGATTGGGAGAGAAAGGTATGGAATTTGTCTGGTATTTTGGGATCTATTGGTAGAGAGGTGGCTGAGCAAATTCAAGCAATGCCAATTGGTGAGATAGATGGTCATGATCGACTCATTTGGCCCCATGATAAGTCAGGGAATTATATTGTGCGATCGGGTTATATTTGGATTCATCACCATGAGGCGGTGGACCTTCTCCGGTGGGCGGCTCAGCCACCACTGCCTCTTCTTGCCGCTTTTATTTCGGATGGACTTACAGCAACAGGGATCGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

912

Amino Acids

103.04

Weight (kDa)

8.74

Isoelectric Point (pI)

36.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 115 - 332 3.3e-09 Endonuclease/Exonuclease/phosphatase family
RVT_1 PF00078 547 - 717 3.4e-16 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000073)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g17821 FvH4_2g04610 FvH4_2g09611 FvH4_2g13563 FvH4_3g21493 FvH4_3g32115 FvH4_3g35060 FvH4_4g00220 FvH4_4g06410 FvH4_4g10367 FvH4_4g10367 FvH4_4g10367 FvH4_5g06101 FvH4_5g08842 FvH4_5g27366 FvH4_5g31283 FvH4_6g11611 FvH4_6g23032 FvH4_7g05661 FvH4_7g06172
malus_domestica MD13G1263700.v1.1
prunus_persica Prupe.1G292300_v2.0.a1 Prupe.5G009700_v2.0.a1 Prupe.6G158100_v2.0.a1 Prupe.8G025100_v2.0.a1 Prupe.8G042600_v2.0.a1
pyrus_communis pycom02g02250 pycom02g16030 pycom05g10490 pycom09g06450 pycom09g06460 pycom13g26200 pycom16g17700
rosa_chinensis RchiOBHm_Chr2g0163321 RchiOBHm_Chr4g0401981 RchiOBHm_Chr5g0005991 RchiOBHm_Chr5g0027191 RchiOBHm_Chr6g0249771
rosa_laevigata RLG00000001040 RLG00000001764 RLG00000002239 RLG00000002240 RLG00000003259 RLG00000008993 RLG00000013847 RLG00000014178 RLG00000015576 RLG00000017269 RLG00000017355 RLG00000018127 RLG00000018602 RLG00000018615 RLG00000019904 RLG00000020044 RLG00000021849 RLG00000021850 RLG00000027965 RLG00000029266 RLG00000029820 RLG00000031112 RLG00000031915 RLG00000033568 RLG00000035013 RLG00000035677 RLG00000036568 RLG00000036628
rosa_multiflora Rmu_co8254085.1_g000001 Rmu_co8462383.1_g000001 Rmu_sc0000185.1_g000049 Rmu_sc0000251.1_g000018 Rmu_sc0000367.1_g000094 Rmu_sc0000498.1_g000023 Rmu_sc0000868.1_g000005 Rmu_sc0001521.1_g000047 Rmu_sc0001534.1_g000007 Rmu_sc0002014.1_g000012 Rmu_sc0003214.1_g000009 Rmu_sc0003573.1_g000041 Rmu_sc0003832.1_g000023 Rmu_sc0004677.1_g000015 Rmu_sc0004967.1_g000014 Rmu_sc0005426.1_g000001 Rmu_sc0005621.1_g000004 Rmu_sc0005640.1_g000009 Rmu_sc0005947.1_g000034 Rmu_sc0006889.1_g000011 Rmu_sc0007121.1_g000014 Rmu_sc0009191.1_g000017 Rmu_sc0010556.1_g000011 Rmu_sc0011396.1_g000007 Rmu_sc0019862.1_g000002 Rmu_sc0022931.1_g000003 Rmu_sc0024164.1_g000001 Rmu_ssc0000391.1_g000027
rosa_roxburghii Rroxscaffold_1G00050060 Rroxscaffold_1G00061810 Rroxscaffold_2G00109590 Rroxscaffold_2G00113810 Rroxscaffold_2G00124040 Rroxscaffold_2G00143620 Rroxscaffold_2G00154760 Rroxscaffold_4G00302820 Rroxscaffold_4G00302830 Rroxscaffold_4G00308680 Rroxscaffold_4G00310240 Rroxscaffold_4G00327890 Rroxscaffold_5G00334600 Rroxscaffold_5G00336050 Rroxscaffold_5G00346560 Rroxscaffold_5G00347860 Rroxscaffold_5G00347870 Rroxscaffold_5G00349330 Rroxscaffold_5G00351980 Rroxscaffold_6G00395280 Rroxscaffold_6G00396750 Rroxscaffold_6G00402910 Rroxscaffold_7G00174790 Rroxscaffold_7G00177570 Rroxscaffold_7G00191820 Rroxscaffold_7G00203480 Rroxscaffold_7G00204380
rosa_rugosa Rorug01G0106600 Rorug01G0106600 Rorug01G0148500.1 Rorug02G0224200 Rorug02G0225500 Rorug02G0233000 Rorug02G0630400 Rorug03G0198000 Rorug03G0232600.1 Rorug03G0240500 Rorug04G0032900 Rorug04G0073300 Rorug04G0118500 Rorug04G0149600.1 Rorug04G0205100 Rorug04G0256700 Rorug05G0020200 Rorug05G0050000 Rorug05G0247300 Rorug05G0295100 Rorug05G0337900 Rorug05G0542600 Rorug06G0034800 Rorug06G0035100 Rorug06G0220100 Rorug07G0091900 Rorug07G0271200
rosa_samantha Rh1AG118200 Rh1AG253000 Rh1AG296300 Rh1AG409000 Rh1AG410600 Rh1BG073400 Rh1BG130400 Rh1BG147300 Rh1BG163600 Rh1CG016000 Rh1DG012200 Rh1DG012300 Rh1DG078600 Rh1DG078800 Rh1DG138100 Rh1DG179200 Rh1DG192600 Rh1DG194500 Rh1DG194600 Rh1DG214300 Rh1DG245900 Rh1DG267000 Rh1DG322700 Rh1DG442700 Rh2AG282000 Rh2AG423000 Rh2AG423100 Rh2AG460700 Rh2AG466200 Rh2AG513400 Rh2AG547000 Rh2BG277800 Rh2BG281200 Rh2BG298300 Rh2BG304300 Rh2BG433500 Rh2CG276000 Rh2CG282600 Rh2CG325500 Rh2CG393000 Rh2DG319200 Rh2DG443400 Rh3AG169300 Rh3AG303600 Rh3BG195000 Rh3BG282600 Rh3BG343400 Rh3BG343500 Rh3BG344100 Rh3CG111700 Rh3CG185700 Rh3CG259100 Rh3CG293000 Rh3CG317900 Rh3CG340900 Rh3CG341000 Rh3CG341100 Rh3CG354700 Rh3CG359200 Rh3CG368100 Rh3DG179900 Rh3DG288700 Rh3DG302600 Rh3DG316000 Rh3DG324500 Rh3DG324600 Rh3DG343200 Rh3DG343300 Rh3DG362400 Rh4AG071800 Rh4BG034000 Rh4BG044300 Rh4BG044400 Rh4BG069600 Rh4BG157400 Rh4CG007000 Rh4CG030400 Rh4CG037900 Rh4CG077700 Rh4CG115000 Rh4CG162400 Rh4CG162500 Rh4CG165500 Rh4CG165600 Rh4CG172900 Rh4CG239700 Rh4DG055900 Rh4DG173200 Rh5AG354900 Rh5AG511100 Rh5BG112600 Rh5BG541000 Rh5BG541100 Rh5BG549300 Rh5CG004400 Rh5CG315000 Rh5CG563800 Rh5CG572100 Rh5DG112200 Rh5DG191300 Rh5DG407600 Rh5DG407700 Rh5DG567500 Rh6AG002600 Rh6AG051400 Rh6AG199800 Rh6AG199900 Rh6AG206200 Rh6AG391000 Rh6AG391100 Rh6BG007500 Rh6BG053400 Rh6BG225900 Rh6BG227100 Rh6BG232400 Rh6CG091100 Rh6CG125400 Rh6CG186700 Rh6CG222700 Rh6CG222800 Rh6CG235500 Rh6CG247200 Rh6CG331200 Rh6CG465100 Rh6DG183500 Rh6DG213500 Rh6DG238800 Rh6DG263700 Rh7AG211800 Rh7AG441800 Rh7BG008600 Rh7BG253000 Rh7BG390000 Rh7CG189600 Rh7CG401500 Rh7CG424200 Rh7CG428700 Rh7CG479800 Rh7CG494300 Rh7DG177000 Rh7DG218700 Rh7DG254000 Rh7DG294800 Rh7DG407200 Rh7DG448800 Rh7DG461500
rosa_wichuraiana Rw0G012180 Rw1G005270 Rw1G012310 Rw1G016890 Rw1G021700 Rw2G014380 Rw2G027690 Rw3G016400 Rw3G028230 Rw3G029320 Rw4G002060 Rw4G002100 Rw4G003980 Rw4G014140 Rw4G014770 Rw4G014780 Rw4G015760 Rw4G021230 Rw5G000520 Rw5G035610 Rw6G001680 Rw6G017890 Rw6G027440 Rw6G034130 Rw6G034170 Rw7G012430 Rw7G033870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 818
AatII GACGTC 1 cut(s) 799
Acc36I ACCTGC 1 cut(s) 865
AccB1I GGYRCC 1 cut(s) 1471
AccI GTMKAC 1 cut(s) 564
AccII CGCG 3 cut(s) 295, 2077, 2425
AccIII TCCGGA 1 cut(s) 1480
AclWI GGATC 7 cut(s) 186, 454, 994, 1154, 1889, 2509, 2735
AcoI YGGCCR 1 cut(s) 950
AcsI RAATTY 6 cut(s) 577, 955, 1146, 2206, 2482, 2529
AcyI GRCGYC 1 cut(s) 796
AfaI GTAC 5 cut(s) 40, 1286, 1514, 1839, 2107
AfiI CCNNNNNNNGG 9 cut(s) 92, 535, 655, 1097, 1876, 1877, 2314, 2644, 2665
AflII CTTAAG 1 cut(s) 1022
AhlI ACTAGT 1 cut(s) 2384
AjnI CCWGG 5 cut(s) 483, 1042, 1251, 1492, 1852
AjuI GAANNNNNNNTTGG 2 cut(s) 1181, 1213
AluBI AGCT 6 cut(s) 321, 932, 1442, 1974, 2123, 2455
AluI AGCT 6 cut(s) 321, 932, 1442, 1974, 2123, 2455
Alw21I GWGCWC 1 cut(s) 922
Alw26I GTCTC 5 cut(s) 1643, 1828, 2225, 2282, 2338
AlwI GGATC 7 cut(s) 186, 454, 994, 1154, 1889, 2509, 2735
Ama87I CYCGRG 2 cut(s) 1780, 1875
Aor13HI TCCGGA 1 cut(s) 1480
ApaI GGGCCC 1 cut(s) 147
ApeKI GCWGC 1 cut(s) 321
ApoI RAATTY 6 cut(s) 577, 955, 1146, 2206, 2482, 2529
AseI ATTAAT 2 cut(s) 555, 1398
AsuC2I CCSGG 7 cut(s) 313, 626, 1180, 1476, 1876, 1877, 2054
AsuHPI GGTGA 7 cut(s) 8, 134, 736, 1271, 1904, 2561, 2627
AvaI CYCGRG 2 cut(s) 1780, 1875
AvaII GGWCC 7 cut(s) 622, 644, 854, 1478, 2324, 2349, 2650
BaeGI GKGCMC 2 cut(s) 147, 1465
BalI TGGCCA 1 cut(s) 952
BanI GGYRCC 1 cut(s) 1471
BanII GRGCYC 1 cut(s) 147
BbsI GAAGAC 2 cut(s) 1441, 1874
Bbv12I GWGCWC 1 cut(s) 922
BbvI GCAGC 1 cut(s) 308
BciT130I CCWGG 5 cut(s) 485, 1044, 1253, 1494, 1854
BclI TGATCA 1 cut(s) 594
BcnI CCSGG 7 cut(s) 313, 626, 1180, 1476, 1876, 1877, 2054
BcoDI GTCTC 5 cut(s) 1643, 1828, 2225, 2282, 2338
BcuI ACTAGT 1 cut(s) 2384
BfaI CTAG 6 cut(s) 365, 873, 1217, 1542, 1709, 2385
BfmI CTRYAG 1 cut(s) 322
BfrI CTTAAG 1 cut(s) 1022
BfuAI ACCTGC 1 cut(s) 865
BglI GCCNNNNNGGC 1 cut(s) 1469
BisI GCNGC 8 cut(s) 296, 305, 322, 370, 1758, 2423, 2667, 2694
BlpI GCTNAGC 2 cut(s) 2523, 2670
BlsI GCNGC 8 cut(s) 297, 306, 323, 371, 1759, 2424, 2668, 2695
BmcAI AGTACT 2 cut(s) 40, 1514
Bme18I GGWCC 7 cut(s) 622, 644, 854, 1478, 2324, 2349, 2650
BmeT110I CYCGRG 2 cut(s) 1780, 1875
BmiI GGNNCC 7 cut(s) 145, 646, 1416, 1473, 2003, 2350, 2582
BmsI GCATC 3 cut(s) 763, 1257, 2026
BoxI GACNNNNGTC 1 cut(s) 996
BpiI GAAGAC 2 cut(s) 1441, 1874
BplI GAGNNNNNCTC 2 cut(s) 1922, 1954
BpmI CTGGAG 2 cut(s) 537, 1299
Bpu1102I GCTNAGC 2 cut(s) 2523, 2670
BpuEI CTTGAG 3 cut(s) 1160, 1451, 1649
BpuMI CCSGG 7 cut(s) 313, 626, 1180, 1476, 1876, 1877, 2054
BsaHI GRCGYC 1 cut(s) 796
BsaJI CCNNGG 9 cut(s) 147, 293, 484, 1179, 1343, 1389, 1875, 1876, 2062
BsaWI WCCGGW 4 cut(s) 1236, 1417, 1480, 2658
Bsc4I CCNNNNNNNGG 9 cut(s) 92, 535, 655, 1097, 1876, 1877, 2314, 2644, 2665
Bse1I ACTGG 3 cut(s) 448, 1453, 1520
Bse3DI GCAATG 1 cut(s) 2544
BseAI TCCGGA 1 cut(s) 1480
BseBI CCWGG 5 cut(s) 485, 1044, 1253, 1494, 1854
BseDI CCNNGG 9 cut(s) 147, 293, 484, 1179, 1343, 1389, 1875, 1876, 2062
BseLI CCNNNNNNNGG 9 cut(s) 92, 535, 655, 1097, 1876, 1877, 2314, 2644, 2665
BseMI GCAATG 1 cut(s) 2544
BseMII CTCAG 5 cut(s) 120, 767, 924, 2514, 2684
BseNI ACTGG 3 cut(s) 448, 1453, 1520
BseRI GAGGAG 2 cut(s) 1237, 1406
BseSI GKGCMC 2 cut(s) 147, 1465
BseXI GCAGC 1 cut(s) 308
BsgI GTGCAG 1 cut(s) 1115
Bsh1236I CGCG 3 cut(s) 295, 2077, 2425
Bsh1285I CGRYCG 2 cut(s) 2173, 2615
BshNI GGYRCC 1 cut(s) 1471
BsiEI CGRYCG 2 cut(s) 2173, 2615
BsiHKAI GWGCWC 1 cut(s) 922
BsiHKCI CYCGRG 2 cut(s) 1780, 1875
BslFI GGGAC 6 cut(s) 630, 710, 719, 797, 1757, 1999
BslI CCNNNNNNNGG 9 cut(s) 92, 535, 655, 1097, 1876, 1877, 2314, 2644, 2665
BsmAI GTCTC 5 cut(s) 1643, 1828, 2225, 2282, 2338
BsmFI GGGAC 6 cut(s) 630, 710, 719, 797, 1757, 1999
BsoBI CYCGRG 2 cut(s) 1780, 1875
Bsp120I GGGCCC 1 cut(s) 143
Bsp1286I GDGCHC 3 cut(s) 147, 922, 1465
Bsp13I TCCGGA 1 cut(s) 1480
Bsp1720I GCTNAGC 2 cut(s) 2523, 2670
BspCNI CTCAG 5 cut(s) 121, 766, 925, 2515, 2683
BspEI TCCGGA 1 cut(s) 1480
BspFNI CGCG 3 cut(s) 295, 2077, 2425
BspHI TCATGA 1 cut(s) 2563
BspLI GGNNCC 7 cut(s) 145, 646, 1416, 1473, 2003, 2350, 2582
BspMAI CTGCAG 1 cut(s) 326
BspMI ACCTGC 1 cut(s) 865
BspPI GGATC 7 cut(s) 186, 454, 994, 1154, 1889, 2509, 2735
BspT107I GGYRCC 1 cut(s) 1471
BspTI CTTAAG 1 cut(s) 1022
BsrDI GCAATG 1 cut(s) 2544
BsrI ACTGG 3 cut(s) 448, 1453, 1520
BssECI CCNNGG 9 cut(s) 147, 293, 484, 1179, 1343, 1389, 1875, 1876, 2062
BssNI GRCGYC 1 cut(s) 796
BssT1I CCWWGG 2 cut(s) 1343, 2062
Bst2UI CCWGG 5 cut(s) 485, 1044, 1253, 1494, 1854
Bst4CI ACNGT 6 cut(s) 356, 382, 1265, 1822, 2105, 2419
Bst6I CTCTTC 4 cut(s) 235, 1422, 2027, 2691
BstACI GRCGYC 1 cut(s) 796
BstAFI CTTAAG 1 cut(s) 1022
BstC8I GCNNGC 3 cut(s) 309, 326, 1641
BstDEI CTNAG 8 cut(s) 129, 583, 753, 933, 1953, 2396, 2523, 2670
BstDSI CCRYGG 1 cut(s) 293
BstENI CCTNNNNNAGG 2 cut(s) 90, 1095
BstFNI CGCG 3 cut(s) 295, 2077, 2425
BstMAI GTCTC 5 cut(s) 1643, 1828, 2225, 2282, 2338
BstMCI CGRYCG 2 cut(s) 2173, 2615
BstMWI GCNNNNNNNGC 7 cut(s) 301, 304, 1175, 1469, 2036, 2271, 2680
BstNI CCWGG 5 cut(s) 485, 1044, 1253, 1494, 1854
BstPAI GACNNNNGTC 1 cut(s) 996
BstSFI CTRYAG 1 cut(s) 322
BstSLI GKGCMC 2 cut(s) 147, 1465
BstUI CGCG 3 cut(s) 295, 2077, 2425
BstV1I GCAGC 1 cut(s) 308
BstV2I GAAGAC 2 cut(s) 1441, 1874
BstX2I RGATCY 3 cut(s) 459, 986, 2501
BstXI CCANNNNNNTGG 1 cut(s) 1350
BstYI RGATCY 3 cut(s) 459, 986, 2501
BtgI CCRYGG 1 cut(s) 293
BtsI GCAGTG 4 cut(s) 372, 545, 1576, 2678
BtsIMutI CAGTG 5 cut(s) 372, 545, 1157, 1576, 2678
BveI ACCTGC 1 cut(s) 865
Cac8I GCNNGC 3 cut(s) 309, 326, 1641
CciI TCATGA 1 cut(s) 2563
Cfr42I CCGCGG 1 cut(s) 296
Cfr9I CCCGGG 1 cut(s) 1875
CpoI CGGWCCG 1 cut(s) 1478
CseI GACGC 1 cut(s) 2066
Csp6I GTAC 5 cut(s) 39, 1285, 1513, 1838, 2106
CspCI CAANNNNNGTGG 2 cut(s) 600, 635
CspI CGGWCCG 1 cut(s) 1478
CviAII CATG 8 cut(s) 155, 359, 1646, 1691, 2011, 2564, 2585, 2639
CviQI GTAC 5 cut(s) 39, 1285, 1513, 1838, 2106
DdeI CTNAG 8 cut(s) 129, 583, 753, 933, 1953, 2396, 2523, 2670
EaeI YGGCCR 1 cut(s) 950
Eam1104I CTCTTC 4 cut(s) 235, 1422, 2027, 2691
EarI CTCTTC 4 cut(s) 235, 1422, 2027, 2691
Eco130I CCWWGG 2 cut(s) 1343, 2062
Eco147I AGGCCT 1 cut(s) 1724
Eco24I GRGCYC 1 cut(s) 147
Eco47I GGWCC 7 cut(s) 622, 644, 854, 1478, 2324, 2349, 2650
Eco88I CYCGRG 2 cut(s) 1780, 1875
EcoNI CCTNNNNNAGG 2 cut(s) 90, 1095
EcoO109I RGGNCCY 5 cut(s) 144, 1100, 2002, 2324, 2349
EcoRII CCWGG 5 cut(s) 483, 1042, 1251, 1492, 1852
EcoT14I CCWWGG 2 cut(s) 1343, 2062
EcoT38I GRGCYC 1 cut(s) 147
ErhI CCWWGG 2 cut(s) 1343, 2062
FaeI CATG 8 cut(s) 158, 362, 1649, 1694, 2014, 2567, 2588, 2642
FalI AAGNNNNNCTT 2 cut(s) 1746, 1778
FaqI GGGAC 6 cut(s) 630, 710, 719, 797, 1757, 1999
FatI CATG 8 cut(s) 154, 358, 1645, 1690, 2010, 2563, 2584, 2638
FauI CCCGC 1 cut(s) 1668
FbaI TGATCA 1 cut(s) 594
FblI GTMKAC 1 cut(s) 564
Fnu4HI GCNGC 8 cut(s) 296, 305, 322, 370, 1758, 2423, 2667, 2694
FriOI GRGCYC 1 cut(s) 147
Fsp4HI GCNGC 8 cut(s) 296, 305, 322, 370, 1758, 2423, 2667, 2694
FspBI CTAG 6 cut(s) 365, 873, 1217, 1542, 1709, 2385
GluI GCNGC 8 cut(s) 296, 305, 322, 370, 1758, 2423, 2667, 2694
GsuI CTGGAG 2 cut(s) 537, 1299
HgaI GACGC 1 cut(s) 2066
Hin1I GRCGYC 1 cut(s) 796
Hin1II CATG 8 cut(s) 158, 362, 1649, 1694, 2014, 2567, 2588, 2642
HincII GTYRAC 3 cut(s) 499, 565, 2382
HindII GTYRAC 3 cut(s) 499, 565, 2382
HpaI GTTAAC 1 cut(s) 2382
HphI GGTGA 7 cut(s) 8, 134, 736, 1271, 1904, 2561, 2627
Hpy166II GTNNAC 5 cut(s) 499, 565, 864, 2382, 2650
Hpy8I GTNNAC 5 cut(s) 499, 565, 864, 2382, 2650
Hpy99I CGWCG 1 cut(s) 801
HpyAV CCTTC 8 cut(s) 871, 882, 1559, 1882, 2143, 2400, 2418, 2663
HpyCH4III ACNGT 6 cut(s) 356, 382, 1265, 1822, 2105, 2419
HpyCH4IV ACGT 3 cut(s) 763, 796, 1359
HpyCH4V TGCA 3 cut(s) 324, 776, 1132
HpyF10VI GCNNNNNNNGC 7 cut(s) 301, 304, 1175, 1469, 2036, 2271, 2680
HpyF3I CTNAG 8 cut(s) 129, 583, 753, 933, 1953, 2396, 2523, 2670
HpySE526I ACGT 3 cut(s) 763, 796, 1359
Hsp92I GRCGYC 1 cut(s) 796
Hsp92II CATG 8 cut(s) 158, 362, 1649, 1694, 2014, 2567, 2588, 2642
Kpn2I TCCGGA 1 cut(s) 1480
Ksp22I TGATCA 1 cut(s) 594
KspAI GTTAAC 1 cut(s) 2382
KspI CCGCGG 1 cut(s) 296
LmnI GCTCC 1 cut(s) 1420
Lsp1109I GCAGC 1 cut(s) 308
LweI GCATC 3 cut(s) 763, 1257, 2026
MaeI CTAG 6 cut(s) 365, 873, 1217, 1542, 1709, 2385
MaeII ACGT 3 cut(s) 763, 796, 1359
MaeIII GTNAC 4 cut(s) 100, 269, 1259, 1813
MfeI CAATTG 2 cut(s) 116, 2544
MflI RGATCY 3 cut(s) 459, 986, 2501
MhlI GDGCHC 3 cut(s) 147, 922, 1465
MlsI TGGCCA 1 cut(s) 952
MluNI TGGCCA 1 cut(s) 952
MlyI GAGTC 6 cut(s) 252, 701, 706, 1067, 2227, 2565
MmeI TCCRAC 1 cut(s) 1404
Mox20I TGGCCA 1 cut(s) 952
MroI TCCGGA 1 cut(s) 1480
MscI TGGCCA 1 cut(s) 952
MslI CAYNNNNRTG 1 cut(s) 2637
Msp20I TGGCCA 1 cut(s) 952
MspA1I CMGCKG 3 cut(s) 295, 321, 932
MspCI CTTAAG 1 cut(s) 1022
MunI CAATTG 2 cut(s) 116, 2544
MvaI CCWGG 5 cut(s) 485, 1044, 1253, 1494, 1854
MvnI CGCG 3 cut(s) 295, 2077, 2425
MwoI GCNNNNNNNGC 7 cut(s) 301, 304, 1175, 1469, 2036, 2271, 2680
NciI CCSGG 7 cut(s) 313, 626, 1180, 1476, 1876, 1877, 2054
NlaIII CATG 8 cut(s) 158, 362, 1649, 1694, 2014, 2567, 2588, 2642
NlaIV GGNNCC 7 cut(s) 145, 646, 1416, 1473, 2003, 2350, 2582
NmeAIII GCCGAG 1 cut(s) 128
NmuCI GTSAC 3 cut(s) 100, 1259, 1813
PaeR7I CTCGAG 1 cut(s) 1780
PagI TCATGA 1 cut(s) 2563
PceI AGGCCT 1 cut(s) 1724
PfoI TCCNGGA 1 cut(s) 1251
PkrI GCNGC 8 cut(s) 297, 306, 323, 371, 1759, 2424, 2668, 2695
Ple19I CGATCG 1 cut(s) 2615
PleI GAGTC 6 cut(s) 251, 701, 706, 1066, 2227, 2565
PpsI GAGTC 6 cut(s) 251, 701, 706, 1066, 2227, 2565
PpuMI RGGWCCY 2 cut(s) 2324, 2349
PshAI GACNNNNGTC 1 cut(s) 996
PshBI ATTAAT 2 cut(s) 555, 1398
PsiI TTATAA 1 cut(s) 818
Psp5II RGGWCCY 2 cut(s) 2324, 2349
Psp6I CCWGG 5 cut(s) 483, 1042, 1251, 1492, 1852
PspGI CCWGG 5 cut(s) 483, 1042, 1251, 1492, 1852
PspN4I GGNNCC 7 cut(s) 145, 646, 1416, 1473, 2003, 2350, 2582
PspOMI GGGCCC 1 cut(s) 143
PspPPI RGGWCCY 2 cut(s) 2324, 2349
PsrI GAACNNNNNNTAC 2 cut(s) 1917, 1949
PstI CTGCAG 1 cut(s) 326
PsuI RGATCY 3 cut(s) 459, 986, 2501
PvuI CGATCG 1 cut(s) 2615
PvuII CAGCTG 2 cut(s) 321, 932
RsaI GTAC 5 cut(s) 40, 1286, 1514, 1839, 2107
RsaNI GTAC 5 cut(s) 39, 1285, 1513, 1838, 2106
RseI CAYNNNNRTG 1 cut(s) 2637
Rsr2I CGGWCCG 1 cut(s) 1478
RsrII CGGWCCG 1 cut(s) 1478
SacII CCGCGG 1 cut(s) 296
SalI GTCGAC 1 cut(s) 563
SatI GCNGC 8 cut(s) 296, 305, 322, 370, 1758, 2423, 2667, 2694
ScaI AGTACT 2 cut(s) 40, 1514
SchI GAGTC 6 cut(s) 252, 701, 706, 1067, 2227, 2565
SduI GDGCHC 3 cut(s) 147, 922, 1465
SfaNI GCATC 3 cut(s) 763, 1257, 2026
SfcI CTRYAG 1 cut(s) 322
Sfr274I CTCGAG 1 cut(s) 1780
Sfr303I CCGCGG 1 cut(s) 296
SgrBI CCGCGG 1 cut(s) 296
SinI GGWCC 7 cut(s) 622, 644, 854, 1478, 2324, 2349, 2650
SlaI CTCGAG 1 cut(s) 1780
SmaI CCCGGG 1 cut(s) 1877
SmiMI CAYNNNNRTG 1 cut(s) 2637
SmlI CTYRAG 5 cut(s) 1022, 1139, 1466, 1664, 1780
SmoI CTYRAG 5 cut(s) 1022, 1139, 1466, 1664, 1780
SpeI ACTAGT 1 cut(s) 2384
SseBI AGGCCT 1 cut(s) 1724
SspI AATATT 2 cut(s) 559, 1633
SspMI CTAG 6 cut(s) 365, 873, 1217, 1542, 1709, 2385
StuI AGGCCT 1 cut(s) 1724
StyI CCWWGG 2 cut(s) 1343, 2062
TaaI ACNGT 6 cut(s) 356, 382, 1265, 1822, 2105, 2419
TaiI ACGT 3 cut(s) 766, 799, 1362
TaqI TCGA 7 cut(s) 217, 231, 564, 710, 1231, 1781, 2569
TaqII GACCGA 1 cut(s) 2187
TatI WGTACW 5 cut(s) 38, 1284, 1512, 1837, 2105
TauI GCSGC 7 cut(s) 298, 307, 372, 1760, 2425, 2669, 2696
TscAI CASTG 5 cut(s) 379, 552, 1157, 1576, 2685
TseFI GTSAC 3 cut(s) 100, 1259, 1813
TseI GCWGC 1 cut(s) 321
Tsp45I GTSAC 3 cut(s) 100, 1259, 1813
TspDTI ATGAA 3 cut(s) 696, 756, 2621
TspGWI ACGGA 3 cut(s) 288, 452, 1873
TspMI CCCGGG 1 cut(s) 1875
TspRI CASTG 5 cut(s) 379, 552, 1157, 1576, 2685
Vha464I CTTAAG 1 cut(s) 1022
VpaK11BI GGWCC 7 cut(s) 622, 644, 854, 1478, 2324, 2349, 2650
VspI ATTAAT 2 cut(s) 555, 1398
XagI CCTNNNNNAGG 2 cut(s) 90, 1095
XapI RAATTY 6 cut(s) 577, 955, 1146, 2206, 2482, 2529
XhoI CTCGAG 1 cut(s) 1780
XmaI CCCGGG 1 cut(s) 1875
XmiI GTMKAC 1 cut(s) 564
XspI CTAG 6 cut(s) 365, 873, 1217, 1542, 1709, 2385
ZraI GACGTC 1 cut(s) 797
ZrmI AGTACT 2 cut(s) 40, 1514
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.