MD01G1221400.v1.1

Cyclic nucleotide-gated ion channel 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0000119
Physical Location & Seq
Reverse (-)
31349917 .. 31352608
2692 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1221400.v1.1.491

Sequence Viewer

Length: 1887 bp
ATGTTTCCCAGAGAGGCTGGAGAACGTTCGAAATCGATGTGCAGCAGGGGCCTTAACTTGATAAGGAAGATTCGTAACTCGCAGCTGGGGCCTTACCTTCCAAGATGGAGCAGTATATATGTGACATCATGCGTATTTGCTGTCTCTCTTGATCCTTTCTTCTTTTATGTTGTGGTCGTCGATCAGAATCAGGATAACAAGTGCTTTGAAATAGACAAAACGTTGGCTATTGTTGTTTCAATTATGCGATCGATCACGGATGTCATTTTCGCAGCGCATTTCATATGTGAAATCTGTAAGGGTGTTCAAAATCCAAATCCAAAAACCCTGAAAAAAGGAGCCTCTTCGAAGATGGAACAAACCGGGAGAAATTCAGATAAGAAAACGAAAATCAGAGAGTTGACTGATCAAGTGGTCATGAAAATAGCTCAGAAGATGCCGTGGTTATCTATCTTTACTGTAATAGACTTTTTCGCTCTTCTTCCCCTCCCACAAGTGCTAATAATAGATATGTTTTACACAGTGGAAGGCTCAGGATATGTGGAATACAACAAGGTCATGAATGTCCTGAATGTTATTCTTCTCTGTCAATATTTTACAAGGATTTATCTACTATCGGCCATAGAATACGAAATGACTAATAGCATATGGGTTAAGGGTCTATACAACTTGTTTCTCTACATTCTTGCCAGCCATGCACTCGGAGCTTTTTGGTACCTTTTTTCCATTCAACGAGAGATATCATGTTGGCACGAGGCTTGTGTAAATCATAGTATAGATCACAGGGAATGTATGAATACTTACTATTGTGATAGTCGGAATACTACGGCAAGAAATATAAGATTTCTAAATAAGCATTGCCCGTTGGATACTCCAGATGGTGCTTTATCTCCGTTTAACTTTGGAATATTTCTTGATTCCTTAAAGAATCTAAACACGGAGCATATAAAGTTCGAAAAGAAGTTCTTTTACTCTTTATGGTGGGGCTTGCGAAATCTGAGTAATTATGGGACCAATCTGACAACAAGTACGTATGTGTGGGAAAACGTGTTTGCGATTTTCATTTCTGTCGTTGGCTTGCTACTGTTTTATTATTTCATTGGAAACATGCAGACTTATATGCAGATAAAAACTACAAAATCAGTGGAGGAGATGAAAACTACAAAATCAGTGGAGGATATGAAACAAGAAGAGTTAAAAAAAGAAGAGTTGAGACAAAAGACTATGCTGAGGAAGCTAGACGCACAGTGTTGGATGGCCACAAATGAAGTCCCTCATGAAATAAGGGAAGAAATCTTGAGATGCATAAACGACCACATTGATGCTGATCTCCATAAGTTTCTATTCTCTTTTCTTCCCGTGAAAACCAGAACATCTCTGAAGCAGTGGCTTTGCATGAAAACACTTAAGAAAATAAAAAAGCTTCAACACATGGATGAAAAAGTGTTGACGTTGATGTGCGGCTATCTGAAGCCAGTGACGTACATTGAGAACAGCATCATTTTTCGAATGGGAGATCTACTCGATAGCATGCTGATCATTATCGAAGGGACAGTGTGGACTTACGCGTCGACTGATAGTCAAGCTGGGCAAGGAATCTCATTAATGGACACCGAGCCCCTCGGGAAAGGTCACTGTTACGGGGAAGAGCTTCTCGATTGCGCATCAGACTGTTTCACAAAGCTTCCAGTCTCTAGCAAGCATGTCAAATGCCACACAAAAATAGAAGCATTTGTGCTCCTGGCCAAGGACTTGGAAACTGTAGTTTCCAGGTACCCGCTACTGTGGCAGAAAAGCGAGAAGGTTTCTCATAAGGTGGAGAACATAGCAGCTTCTACCATAGCAAAAGCATACCGTCATCATCTAGAGCGGCGGGCTGAGGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
Ion_trans cNMP_binding

Protein Analysis

629

Amino Acids

72.78

Weight (kDa)

8.65

Isoelectric Point (pI)

40.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ion_trans PF00520 68 - 376 1.6e-11 Ion transport protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000119)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g10570 FvH4_4g36390 FvH4_7g31840 FvH4_7g31840 FvH4_7g31850 FvH4_7g31850 FvH4_7g31861 FvH4_7g31861 FvH4_7g31950
malus_domestica MD01G1054600.v1.1 MD01G1221100.v1.1 MD01G1221400.v1.1 MD01G1221600.v1.1 MD01G1221800.v1.1 MD01G1222000.v1.1 MD01G1222100.v1.1 MD07G1140800.v1.1 MD07G1291200.v1.1 MD07G1291400.v1.1 MD07G1291500.v1.1 MD07G1291700.v1.1 MD07G1292500.v1.1 MD07G1292700.v1.1 MD07G1292800.v1.1 MD07G1292900.v1.1 MD08G1241600.v1.1 MD11G1083800.v1.1 MD15G1431600.v1.1
prunus_persica Prupe.1G017800_v2.0.a1 Prupe.1G017800_v2.0.a1 Prupe.1G162800_v2.0.a1 Prupe.2G312500_v2.0.a1 Prupe.2G312500_v2.0.a1 Prupe.2G312700_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092800_v2.0.a1 Prupe.4G092800_v2.0.a1 Prupe.6G142800_v2.0.a1 Prupe.6G142800_v2.0.a1 Prupe.6G143000_v2.0.a1 Prupe.6G143100_v2.0.a1 Prupe.6G143100_v2.0.a1 Prupe.6G143100_v2.0.a1 Prupe.6G143100_v2.0.a1
pyrus_communis pycom01g23050 pycom01g23070 pycom01g23090 pycom01g23100 pycom01g23110 pycom01g23120 pycom01g23170 pycom07g26410 pycom07g26440 pycom07g26470 pycom07g26480 pycom07g26490 pycom07g26500 pycom07g26510 pycom07g26530 pycom07g26540 pycom07g26570 pycom07g26580 pycom07g26590 pycom08g20940
rosa_chinensis RchiOBHm_Chr1g0324571 RchiOBHm_Chr1g0336181 RchiOBHm_Chr1g0381061 RchiOBHm_Chr1g0381091 RchiOBHm_Chr1g0381121 RchiOBHm_Chr1g0381131 RchiOBHm_Chr1g0381141 RchiOBHm_Chr1g0381161 RchiOBHm_Chr1g0381211 RchiOBHm_Chr2g0110361 RchiOBHm_Chr2g0120231 RchiOBHm_Chr3g0486281 RchiOBHm_Chr3g0486311 RchiOBHm_Chr3g0486401 RchiOBHm_Chr4g0409511 RchiOBHm_Chr4g0409521 RchiOBHm_Chr5g0017271 RchiOBHm_Chr5g0048781 RchiOBHm_Chr5g0048811
rosa_laevigata RLG00000002401 RLG00000008559 RLG00000017798 RLG00000017799 RLG00000023082 RLG00000023088 RLG00000026230 RLG00000026238 RLG00000026240 RLG00000026241 RLG00000026242 RLG00000026243 RLG00000026244 RLG00000026246 RLG00000026247 RLG00000026248 RLG00000029876 RLG00000030189 RLG00000034579
rosa_multiflora Rmu_co8101374.1_g000001 Rmu_co8309149.1_g000001 Rmu_co8450063.1_g000001 Rmu_sc0000441.1_g000094 Rmu_sc0000479.1_g000005 Rmu_sc0000647.1_g000006 Rmu_sc0001393.1_g000031 Rmu_sc0001423.1_g000037 Rmu_sc0001616.1_g000015 Rmu_sc0001857.1_g000011 Rmu_sc0002183.1_g000013 Rmu_sc0002183.1_g000028 Rmu_sc0002183.1_g000030 Rmu_sc0002688.1_g000003 Rmu_sc0002688.1_g000020 Rmu_sc0003801.1_g000003 Rmu_sc0003801.1_g000004 Rmu_sc0004125.1_g000006 Rmu_sc0004494.1_g000005 Rmu_sc0004494.1_g000006 Rmu_sc0004494.1_g000008 Rmu_sc0004494.1_g000009 Rmu_sc0005842.1_g000004 Rmu_sc0006872.1_g000001 Rmu_sc0006872.1_g000005 Rmu_sc0006872.1_g000009 Rmu_sc0006872.1_g000010 Rmu_sc0008053.1_g000026
rosa_roxburghii Rroxscaffold_159G00432960 Rroxscaffold_1G00033060 Rroxscaffold_2G00123150 Rroxscaffold_2G00123170 Rroxscaffold_2G00133500 Rroxscaffold_2G00133550 Rroxscaffold_4G00278650 Rroxscaffold_4G00278750 Rroxscaffold_4G00278790 Rroxscaffold_4G00278800 Rroxscaffold_4G00278810 Rroxscaffold_4G00325390 Rroxscaffold_5G00354010 Rroxscaffold_6G00395890 Rroxscaffold_6G00395900 Rroxscaffold_6G00395920
rosa_rugosa Rorug01G0046900.1 Rorug01G0427000 Rorug01G0427100 Rorug01G0427200 Rorug01G0427300 Rorug01G0427400 Rorug01G0427500 Rorug01G0427500 Rorug01G0427600 Rorug01G0427600 Rorug01G0427700 Rorug01G0427700 Rorug01G0427800 Rorug01G0428700 Rorug02G0166400 Rorug02G0166500 Rorug02G0166500 Rorug02G0166600 Rorug02G0166600 Rorug02G0166700 Rorug02G0167300 Rorug02G0167300 Rorug02G0167300 Rorug03G0224600 Rorug03G0224600 Rorug04G0094400 Rorug05G0241100 Rorug05G0241200
rosa_samantha Rh1BG407700 Rh1BG407900 Rh1BG409000 Rh1CG064700 Rh1CG420900 Rh1CG421000 Rh1CG421200 Rh1CG421300 Rh1CG421800 Rh1CG421900 Rh1CG422000 Rh1CG422200 Rh1CG422300 Rh1CG423600 Rh1DG437600 Rh1DG438900 Rh1DG439000 Rh1DG440300 Rh2AG219100 Rh2CG220700 Rh2DG225300 Rh3AG274400 Rh3AG274600 Rh3CG308900 Rh3DG305800 Rh4AG156400 Rh5AG129000 Rh5AG320900 Rh5CG139600
rosa_wichuraiana Rw1G005300 Rw1G039280 Rw1G039300 Rw1G039310 Rw1G039320 Rw1G039330 Rw1G039340 Rw1G039360 Rw1G039390 Rw2G016910 Rw2G022800 Rw3G024330 Rw3G024340 Rw3G024350 Rw3G024400 Rw4G012810 Rw5G030120 Rw5G030280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1567
Acc16I TGCGCA 1 cut(s) 1663
Acc65I GGTACC 2 cut(s) 714, 1773
AccB1I GGYRCC 2 cut(s) 714, 1773
AccBSI CCGCTC 1 cut(s) 1870
AccI GTMKAC 1 cut(s) 1571
AccII CGCG 1 cut(s) 1568
AciI CCGC 4 cut(s) 1461, 1778, 1870, 1873
AclI AACGTT 2 cut(s) 25, 221
AclWI GGATC 1 cut(s) 146
AcoI YGGCCR 3 cut(s) 618, 1257, 1743
AcsI RAATTY 1 cut(s) 370
AcuI CTGAAG 2 cut(s) 1400, 1490
AfaI GTAC 4 cut(s) 716, 1030, 1484, 1775
AfiI CCNNNNNNNGG 1 cut(s) 1747
AflII CTTAAG 1 cut(s) 1406
AflIII ACRYGT 2 cut(s) 1047, 1566
AgsI TTSAA 5 cut(s) 209, 240, 308, 731, 1427
AhdI GACNNNNNGTC 1 cut(s) 1578
AjnI CCWGG 2 cut(s) 1740, 1769
AluBI AGCT 9 cut(s) 85, 428, 707, 1237, 1423, 1586, 1651, 1684, 1832
AluI AGCT 9 cut(s) 85, 428, 707, 1237, 1423, 1586, 1651, 1684, 1832
Alw21I GWGCWC 1 cut(s) 1740
Alw26I GTCTC 3 cut(s) 148, 1207, 1696
AlwI GGATC 1 cut(s) 146
Ama87I CYCGRG 1 cut(s) 1622
AoxI GGCC 6 cut(s) 49, 89, 618, 1257, 1743, 1881
ApeKI GCWGC 4 cut(s) 42, 82, 272, 1829
ApoI RAATTY 1 cut(s) 370
AseI ATTAAT 1 cut(s) 1604
Asp700I GAANNNNTTC 1 cut(s) 1650
Asp718I GGTACC 2 cut(s) 714, 1773
AspLEI GCGC 2 cut(s) 277, 1664
AspS9I GGNCC 3 cut(s) 49, 89, 1011
AsuC2I CCSGG 1 cut(s) 364
AsuII TTCGAA 4 cut(s) 29, 347, 954, 1507
AvaI CYCGRG 1 cut(s) 1622
AvaII GGWCC 1 cut(s) 1011
BalI TGGCCA 2 cut(s) 1259, 1745
BanI GGYRCC 2 cut(s) 714, 1773
BanII GRGCYC 1 cut(s) 1620
BarI GAAGNNNNNNTAC 2 cut(s) 953, 985
BauI CACGAG 1 cut(s) 752
Bbv12I GWGCWC 1 cut(s) 1740
BbvCI CCTCAGC 2 cut(s) 1229, 1878
BbvI GCAGC 4 cut(s) 54, 94, 284, 1841
BccI CCATC 4 cut(s) 99, 346, 872, 1249
BceAI ACGGC 2 cut(s) 424, 843
BciT130I CCWGG 2 cut(s) 1742, 1771
BciVI GTATCC 1 cut(s) 862
BclI TGATCA 2 cut(s) 406, 1536
BcnI CCSGG 1 cut(s) 364
BcoDI GTCTC 3 cut(s) 148, 1207, 1696
BfaI CTAG 3 cut(s) 1238, 1695, 1865
BfmI CTRYAG 1 cut(s) 1761
BfrI CTTAAG 1 cut(s) 1406
BfuI GTATCC 1 cut(s) 862
BglII AGATCT 1 cut(s) 1516
BisI GCNGC 6 cut(s) 43, 83, 273, 1462, 1830, 1871
BlsI GCNGC 6 cut(s) 44, 84, 274, 1463, 1831, 1872
Bme1390I CCNGG 3 cut(s) 364, 1742, 1771
Bme18I GGWCC 1 cut(s) 1011
BmeRI GACNNNNNGTC 1 cut(s) 1578
BmeT110I CYCGRG 1 cut(s) 1622
BmgT120I GGNCC 3 cut(s) 49, 89, 1011
BmiI GGNNCC 6 cut(s) 50, 90, 340, 716, 1012, 1775
BmrFI CCNGG 3 cut(s) 364, 1742, 1771
BmsI GCATC 5 cut(s) 426, 1292, 1312, 1506, 1673
BplI GAGNNNNNCTC 2 cut(s) 1506, 1538
BpmI CTGGAG 2 cut(s) 39, 858
Bpu10I CCTNAGC 3 cut(s) 532, 1229, 1878
Bpu14I TTCGAA 4 cut(s) 29, 347, 954, 1507
BpuEI CTTGAG 1 cut(s) 1318
BpuMI CCSGG 1 cut(s) 364
Bsa29I ATCGAT 2 cut(s) 35, 251
BsaAI YACGTR 1 cut(s) 1032
BsaBI GATNNNNATC 3 cut(s) 186, 1326, 1541
BsaJI CCNNGG 3 cut(s) 440, 1621, 1746
Bsc4I CCNNNNNNNGG 1 cut(s) 1747
Bse1I ACTGG 2 cut(s) 1475, 1688
Bse3DI GCAATG 1 cut(s) 856
Bse8I GATNNNNATC 3 cut(s) 186, 1326, 1541
BseBI CCWGG 2 cut(s) 1742, 1771
BseCI ATCGAT 2 cut(s) 35, 251
BseDI CCNNGG 3 cut(s) 440, 1621, 1746
BseGI GGATG 3 cut(s) 265, 1260, 1441
BseJI GATNNNNATC 3 cut(s) 186, 1326, 1541
BseLI CCNNNNNNNGG 1 cut(s) 1747
BseMI GCAATG 1 cut(s) 856
BseMII CTCAG 5 cut(s) 443, 546, 989, 1220, 1869
BseNI ACTGG 2 cut(s) 1475, 1688
BseRI GAGGAG 1 cut(s) 1163
BseXI GCAGC 4 cut(s) 54, 94, 284, 1841
BseYI CCCAGC 2 cut(s) 85, 1586
BsgI GTGCAG 1 cut(s) 61
Bsh1236I CGCG 1 cut(s) 1568
Bsh1285I CGRYCG 1 cut(s) 251
BshFI GGCC 6 cut(s) 51, 91, 620, 1259, 1745, 1883
BshNI GGYRCC 2 cut(s) 714, 1773
BshVI ATCGAT 2 cut(s) 35, 251
BsiEI CGRYCG 1 cut(s) 251
BsiHKAI GWGCWC 1 cut(s) 1740
BsiHKCI CYCGRG 1 cut(s) 1622
BsiSI CCGG 1 cut(s) 363
BslFI GGGAC 3 cut(s) 1024, 1256, 1564
BslI CCNNNNNNNGG 1 cut(s) 1747
BsmAI GTCTC 3 cut(s) 148, 1207, 1696
BsmFI GGGAC 3 cut(s) 1024, 1256, 1564
BsnI GGCC 6 cut(s) 51, 91, 620, 1259, 1745, 1883
BsoBI CYCGRG 1 cut(s) 1622
Bsp119I TTCGAA 4 cut(s) 29, 347, 954, 1507
Bsp1286I GDGCHC 2 cut(s) 1620, 1740
Bsp143I GATC 9 cut(s) 151, 181, 248, 252, 406, 778, 1327, 1516, 1536
BspACI CCGC 4 cut(s) 1461, 1778, 1870, 1873
BspANI GGCC 6 cut(s) 51, 91, 620, 1259, 1745, 1883
BspCNI CTCAG 5 cut(s) 442, 545, 990, 1221, 1870
BspDI ATCGAT 2 cut(s) 35, 251
BspFNI CGCG 1 cut(s) 1568
BspHI TCATGA 3 cut(s) 417, 558, 1276
BspLI GGNNCC 6 cut(s) 50, 90, 340, 716, 1012, 1775
BspPI GGATC 1 cut(s) 146
BspQI GCTCTTC 2 cut(s) 483, 1641
BspT104I TTCGAA 4 cut(s) 29, 347, 954, 1507
BspT107I GGYRCC 2 cut(s) 714, 1773
BspTI CTTAAG 1 cut(s) 1406
BsrBI CCGCTC 1 cut(s) 1870
BsrDI GCAATG 1 cut(s) 856
BsrI ACTGG 2 cut(s) 1475, 1688
BssECI CCNNGG 3 cut(s) 440, 1621, 1746
BssMI GATC 9 cut(s) 151, 181, 248, 252, 406, 778, 1327, 1516, 1536
BssSI CACGAG 1 cut(s) 752
BssT1I CCWWGG 1 cut(s) 1746
Bst2BI CACGAG 1 cut(s) 752
Bst2UI CCWGG 2 cut(s) 1742, 1771
Bst6I CTCTTC 5 cut(s) 349, 483, 1185, 1200, 1641
BstAFI CTTAAG 1 cut(s) 1406
BstBAI YACGTR 1 cut(s) 1032
BstBI TTCGAA 4 cut(s) 29, 347, 954, 1507
BstC8I GCNNGC 6 cut(s) 691, 989, 1079, 1532, 1700, 1875
BstDEI CTNAG 5 cut(s) 429, 532, 998, 1229, 1878
BstDSI CCRYGG 1 cut(s) 440
BstENI CCTNNNNNAGG 1 cut(s) 1745
BstF5I GGATG 3 cut(s) 265, 1260, 1441
BstFNI CGCG 1 cut(s) 1568
BstHHI GCGC 2 cut(s) 277, 1664
BstKTI GATC 9 cut(s) 154, 184, 251, 255, 409, 781, 1330, 1519, 1539
BstMAI GTCTC 3 cut(s) 148, 1207, 1696
BstMBI GATC 9 cut(s) 151, 181, 248, 252, 406, 778, 1327, 1516, 1536
BstMCI CGRYCG 1 cut(s) 251
BstMWI GCNNNNNNNGC 6 cut(s) 48, 88, 695, 704, 1234, 1786
BstNI CCWGG 2 cut(s) 1742, 1771
BstNSI RCATGY 3 cut(s) 1111, 1534, 1706
BstSCI CCNGG 3 cut(s) 362, 1740, 1769
BstSFI CTRYAG 1 cut(s) 1761
BstSNI TACGTA 1 cut(s) 1032
BstUI CGCG 1 cut(s) 1568
BstV1I GCAGC 4 cut(s) 54, 94, 284, 1841
BstX2I RGATCY 1 cut(s) 1516
BstXI CCANNNNNNTGG 1 cut(s) 1753
BstYI RGATCY 1 cut(s) 1516
Bsu15I ATCGAT 2 cut(s) 35, 251
BsuI GTATCC 1 cut(s) 862
BsuRI GGCC 6 cut(s) 51, 91, 620, 1259, 1745, 1883
BsuTUI ATCGAT 2 cut(s) 35, 251
BtgI CCRYGG 1 cut(s) 440
BtsCI GGATG 3 cut(s) 265, 1260, 1441
BtsI GCAGTG 1 cut(s) 1391
BtsIMutI CAGTG 8 cut(s) 528, 1149, 1176, 1253, 1391, 1482, 1560, 1633
Cac8I GCNNGC 6 cut(s) 691, 989, 1079, 1532, 1700, 1875
CciI TCATGA 3 cut(s) 417, 558, 1276
CfoI GCGC 2 cut(s) 277, 1664
Cfr13I GGNCC 3 cut(s) 49, 89, 1011
ClaI ATCGAT 2 cut(s) 35, 251
CseI GACGC 2 cut(s) 1250, 1557
Csp6I GTAC 4 cut(s) 715, 1029, 1483, 1774
CspCI CAANNNNNGTGG 4 cut(s) 1125, 1152, 1160, 1187
CviQI GTAC 4 cut(s) 715, 1029, 1483, 1774
DdeI CTNAG 5 cut(s) 429, 532, 998, 1229, 1878
DpnI GATC 9 cut(s) 153, 183, 250, 254, 408, 780, 1329, 1518, 1538
DpnII GATC 9 cut(s) 151, 181, 248, 252, 406, 778, 1327, 1516, 1536
DrdI GACNNNNNNGTC 1 cut(s) 1567
DriI GACNNNNNGTC 1 cut(s) 1578
DseDI GACNNNNNNGTC 1 cut(s) 1567
EaeI YGGCCR 3 cut(s) 618, 1257, 1743
Eam1104I CTCTTC 5 cut(s) 349, 483, 1185, 1200, 1641
Eam1105I GACNNNNNGTC 1 cut(s) 1578
EarI CTCTTC 5 cut(s) 349, 483, 1185, 1200, 1641
Eco105I TACGTA 1 cut(s) 1032
Eco130I CCWWGG 1 cut(s) 1746
Eco147I AGGCCT 1 cut(s) 1883
Eco24I GRGCYC 1 cut(s) 1620
Eco32I GATATC 1 cut(s) 741
Eco47I GGWCC 1 cut(s) 1011
Eco57I CTGAAG 2 cut(s) 1400, 1490
Eco88I CYCGRG 1 cut(s) 1622
EcoNI CCTNNNNNAGG 1 cut(s) 1745
EcoO109I RGGNCCY 2 cut(s) 49, 89
EcoRII CCWGG 2 cut(s) 1740, 1769
EcoRV GATATC 1 cut(s) 741
EcoT14I CCWWGG 1 cut(s) 1746
EcoT22I ATGCAT 1 cut(s) 1307
EcoT38I GRGCYC 1 cut(s) 1620
ErhI CCWWGG 1 cut(s) 1746
FalI AAGNNNNNCTT 2 cut(s) 950, 982
FaqI GGGAC 3 cut(s) 1024, 1256, 1564
FauI CCCGC 2 cut(s) 1785, 1866
FauNDI CATATG 2 cut(s) 284, 647
FbaI TGATCA 2 cut(s) 406, 1536
FblI GTMKAC 1 cut(s) 1571
Fnu4HI GCNGC 6 cut(s) 43, 83, 273, 1462, 1830, 1871
FokI GGATG 3 cut(s) 272, 1267, 1448
FriOI GRGCYC 1 cut(s) 1620
Fsp4HI GCNGC 6 cut(s) 43, 83, 273, 1462, 1830, 1871
FspBI CTAG 3 cut(s) 1238, 1695, 1865
FspI TGCGCA 1 cut(s) 1663
GlaI GCGC 2 cut(s) 276, 1663
GluI GCNGC 6 cut(s) 43, 83, 273, 1462, 1830, 1871
GsaI CCCAGC 2 cut(s) 89, 1590
GsuI CTGGAG 2 cut(s) 39, 858
HaeIII GGCC 6 cut(s) 51, 91, 620, 1259, 1745, 1883
HapII CCGG 1 cut(s) 363
HgaI GACGC 2 cut(s) 1250, 1557
HhaI GCGC 2 cut(s) 277, 1664
Hin6I GCGC 2 cut(s) 275, 1662
HinP1I GCGC 2 cut(s) 275, 1662
HincII GTYRAC 3 cut(s) 402, 1449, 1572
HindII GTYRAC 3 cut(s) 402, 1449, 1572
HindIII AAGCTT 2 cut(s) 1421, 1682
HinfI GANTC 5 cut(s) 70, 187, 917, 928, 1596
HpaII CCGG 1 cut(s) 363
Hpy166II GTNNAC 4 cut(s) 402, 1449, 1560, 1572
Hpy8I GTNNAC 4 cut(s) 402, 1449, 1560, 1572
Hpy99I CGWCG 2 cut(s) 182, 1573
HpyAV CCTTC 4 cut(s) 107, 521, 1541, 1795
HpyCH4IV ACGT 6 cut(s) 25, 221, 1031, 1047, 1451, 1481
HpyCH4V TGCA 6 cut(s) 42, 698, 1111, 1123, 1305, 1395
HpyF10VI GCNNNNNNNGC 6 cut(s) 48, 88, 695, 704, 1234, 1786
HpyF3I CTNAG 5 cut(s) 429, 532, 998, 1229, 1878
HpySE526I ACGT 6 cut(s) 25, 221, 1031, 1047, 1451, 1481
HspAI GCGC 2 cut(s) 275, 1662
KpnI GGTACC 2 cut(s) 718, 1777
Ksp22I TGATCA 2 cut(s) 406, 1536
Kzo9I GATC 9 cut(s) 151, 181, 248, 252, 406, 778, 1327, 1516, 1536
LguI GCTCTTC 2 cut(s) 483, 1641
LmnI GCTCC 5 cut(s) 108, 338, 704, 940, 1743
Lsp1109I GCAGC 4 cut(s) 54, 94, 284, 1841
LweI GCATC 5 cut(s) 426, 1292, 1312, 1506, 1673
MaeI CTAG 3 cut(s) 1238, 1695, 1865
MaeII ACGT 6 cut(s) 25, 221, 1031, 1047, 1451, 1481
MaeIII GTNAC 5 cut(s) 74, 121, 1477, 1631, 1637
MalI GATC 9 cut(s) 153, 183, 250, 254, 408, 780, 1329, 1518, 1538
MbiI CCGCTC 1 cut(s) 1870
MboI GATC 9 cut(s) 151, 181, 248, 252, 406, 778, 1327, 1516, 1536
MflI RGATCY 1 cut(s) 1516
MhlI GDGCHC 2 cut(s) 1620, 1740
MlsI TGGCCA 2 cut(s) 1259, 1745
MluCI AATT 3 cut(s) 240, 370, 1003
MluI ACGCGT 1 cut(s) 1566
MluNI TGGCCA 2 cut(s) 1259, 1745
MmeI TCCRAC 3 cut(s) 797, 846, 1232
Mox20I TGGCCA 2 cut(s) 1259, 1745
Mph1103I ATGCAT 1 cut(s) 1307
MroXI GAANNNNTTC 1 cut(s) 1650
MscI TGGCCA 2 cut(s) 1259, 1745
MseI TTAA 7 cut(s) 54, 654, 897, 923, 1196, 1407, 1604
MslI CAYNNNNRTG 2 cut(s) 1320, 1434
Msp20I TGGCCA 2 cut(s) 1259, 1745
MspA1I CMGCKG 1 cut(s) 85
MspCI CTTAAG 1 cut(s) 1406
MspI CCGG 1 cut(s) 363
MspR9I CCNGG 3 cut(s) 364, 1742, 1771
MvaI CCWGG 2 cut(s) 1742, 1771
MvnI CGCG 1 cut(s) 1568
MwoI GCNNNNNNNGC 6 cut(s) 48, 88, 695, 704, 1234, 1786
NciI CCSGG 1 cut(s) 364
NdeI CATATG 2 cut(s) 284, 647
NdeII GATC 9 cut(s) 151, 181, 248, 252, 406, 778, 1327, 1516, 1536
NlaIV GGNNCC 6 cut(s) 50, 90, 340, 716, 1012, 1775
NmuCI GTSAC 3 cut(s) 121, 1477, 1631
NsbI TGCGCA 1 cut(s) 1663
NsiI ATGCAT 1 cut(s) 1307
NspI RCATGY 3 cut(s) 1111, 1534, 1706
NspV TTCGAA 4 cut(s) 29, 347, 954, 1507
PaeI GCATGC 1 cut(s) 1534
PagI TCATGA 3 cut(s) 417, 558, 1276
PceI AGGCCT 1 cut(s) 1883
PciSI GCTCTTC 2 cut(s) 483, 1641
PdmI GAANNNNTTC 1 cut(s) 1650
PfeI GAWTC 5 cut(s) 70, 187, 917, 928, 1596
PkrI GCNGC 6 cut(s) 44, 84, 274, 1463, 1831, 1872
Ple19I CGATCG 1 cut(s) 251
Ppu21I YACGTR 1 cut(s) 1032
PshBI ATTAAT 1 cut(s) 1604
Psp1406I AACGTT 2 cut(s) 25, 221
Psp6I CCWGG 2 cut(s) 1740, 1769
PspFI CCCAGC 2 cut(s) 85, 1586
PspGI CCWGG 2 cut(s) 1740, 1769
PspN4I GGNNCC 6 cut(s) 50, 90, 340, 716, 1012, 1775
PspPI GGNCC 3 cut(s) 49, 89, 1011
PsuI RGATCY 1 cut(s) 1516
PvuI CGATCG 1 cut(s) 251
PvuII CAGCTG 1 cut(s) 85
RsaI GTAC 4 cut(s) 716, 1030, 1484, 1775
RsaNI GTAC 4 cut(s) 715, 1029, 1483, 1774
RseI CAYNNNNRTG 2 cut(s) 1320, 1434
SalI GTCGAC 1 cut(s) 1570
SapI GCTCTTC 2 cut(s) 483, 1641
SaqAI TTAA 7 cut(s) 54, 654, 897, 923, 1196, 1407, 1604
SatI GCNGC 6 cut(s) 43, 83, 273, 1462, 1830, 1871
Sau3AI GATC 9 cut(s) 151, 181, 248, 252, 406, 778, 1327, 1516, 1536
Sau96I GGNCC 3 cut(s) 49, 89, 1011
ScrFI CCNGG 3 cut(s) 364, 1742, 1771
SduI GDGCHC 2 cut(s) 1620, 1740
SfaNI GCATC 5 cut(s) 426, 1292, 1312, 1506, 1673
SfcI CTRYAG 1 cut(s) 1761
SfuI TTCGAA 4 cut(s) 29, 347, 954, 1507
SinI GGWCC 1 cut(s) 1011
SmiMI CAYNNNNRTG 2 cut(s) 1320, 1434
SmlI CTYRAG 2 cut(s) 1297, 1406
SmoI CTYRAG 2 cut(s) 1297, 1406
SnaBI TACGTA 1 cut(s) 1032
SphI GCATGC 1 cut(s) 1534
Sse9I AATT 3 cut(s) 240, 370, 1003
SseBI AGGCCT 1 cut(s) 1883
SsiI CCGC 4 cut(s) 1461, 1778, 1870, 1873
SspI AATATT 2 cut(s) 593, 909
SspMI CTAG 3 cut(s) 1238, 1695, 1865
StuI AGGCCT 1 cut(s) 1883
StyD4I CCNGG 3 cut(s) 362, 1740, 1769
StyI CCWWGG 1 cut(s) 1746
TaiI ACGT 6 cut(s) 28, 224, 1034, 1050, 1454, 1484
TasI AATT 3 cut(s) 240, 370, 1003
TauI GCSGC 2 cut(s) 1464, 1873
TfiI GAWTC 5 cut(s) 70, 187, 917, 928, 1596
Tru1I TTAA 7 cut(s) 54, 654, 897, 923, 1196, 1407, 1604
Tru9I TTAA 7 cut(s) 54, 654, 897, 923, 1196, 1407, 1604
TscAI CASTG 8 cut(s) 528, 1149, 1176, 1253, 1391, 1482, 1560, 1640
TseFI GTSAC 3 cut(s) 121, 1477, 1631
TseI GCWGC 4 cut(s) 42, 82, 272, 1829
Tsp45I GTSAC 3 cut(s) 121, 1477, 1631
TspGWI ACGGA 3 cut(s) 272, 882, 953
TspRI CASTG 8 cut(s) 528, 1149, 1176, 1253, 1391, 1482, 1560, 1640
Vha464I CTTAAG 1 cut(s) 1406
VpaK11BI GGWCC 1 cut(s) 1011
VspI ATTAAT 1 cut(s) 1604
XagI CCTNNNNNAGG 1 cut(s) 1745
XapI RAATTY 1 cut(s) 370
XbaI TCTAGA 1 cut(s) 1864
XceI RCATGY 3 cut(s) 1111, 1534, 1706
XmiI GTMKAC 1 cut(s) 1571
XmnI GAANNNNTTC 1 cut(s) 1650
XspI CTAG 3 cut(s) 1238, 1695, 1865
Zsp2I ATGCAT 1 cut(s) 1307
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.