MD07G1292500.v1.1

Cyclic nucleotide-gated ion channel 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0000119
Physical Location & Seq
Reverse (-)
35367055 .. 35370360
3306 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1292500.v1.1.491

Sequence Viewer

Length: 1890 bp
ATGATGCATGTCCCAAATAATGAGTACACCATTGAATTAAGCCAAACACCTTTCAATCCCAACAGACTTAACCCGCAACCTGGAGTAGCTTCACCTAGTACAAAATTTAAAGGGCCAAAACTGGATCGACTTCTTGCAAATGATCTACTGTGGAACGAAATATTTGTACTTTCATGTGCGTTTGCTGTGTCACTGGACCCTCTCTTCTTTTACATTCCATACATCGATGAAAAAAATAAGTGCCTTGGAATGGACAAGAAGCTGAAGAATGCAACTCTTGTTTTGCGACTGCTCACAGATACCATTTTCGTAGTGCATATTATTCATCAAATTCGTCAGGCCATTAAATCTGTCAACTCACATGAAATGGAAAATCCCGGTTGGGAAGCTATGGCAGTAGCCAGAAAGTTGTCGTGGCGCTCGATCGTTACTGACATTCTTGCTGTTCTTCCCATCCCACAGGTGCTTTTAGTAGGTGTTTACTTCAAAATTAAAGTATCTGGATATGTGGTCAAAAGATCGGTTCTATCTTTTTTCCTTCTTGCCCAATATCTTCCGAGGATTTATCGAATTCACCTATCCTCTCACAACCTTACACGAACTGGCATATGGGCCAAATGTGCATTCAATTTGTTCCTTTACATCATTGCAAGTCATGTACTTGGAGCCTTTTGGTATTTTTTTTCTATTCAACGAGAGACATCATGTTGGTATCGGGCATTAAAAAAACCTAACATGACAGACTTGGGTACTTTTTACTGTGACGACCGTGCTACGGCAAGAAATAATATATCATTTCTAGATGAATCTTGTCCCATAAATGTTCCTGCAAATACTACACCGCCGTTTGATTTCGGAATATTTCTTGATTCTCTTCAATCTGGAAACGCGGCGTCGTTGGATATTCTGGGCAAGGTGATCTACTCGTCTTGGTGGGGACTGCGAAATTTAAGCAACTTTGGCACAAATCTGACAACAAGTACATATGTGTGGGAAAACATCTTTGCAATTCTTATTTCTATCATTGGAATATTCCTATTTTTATATCTCATAGGAAATGCACAGACGTTTATGCAATTGGCAACTACAAGGTCGGAGGATATAAGACAGAAGATTAAGATGAAAGAGCGACATATAGAAGAGTGGATGGAGAAAAATGATCTCCCAGAAGATTTTAAAAAAGAGATCAAGAAAAACGTAAAACAAAAACTGAAAGAAAACAAAGACGCTGATCTGGGGAATCTGTTCTCCATTCTTCCTTGGTACACCATGAAACCCCTAAAGCGTTTTCTGTTCATGAATACCCTAAGGACAGTACCAATGCTCAAAGAAATGGATGAAAAAGTGTTGAAAATGATCTGCGACTATCTGAGGCCAGTGACGTACGACGAGAATAGCTTCGTTGTGCGAACAGGAGACATTCTCGATCGGATGATCTTCATTACGGAAGGGTTTATATGGACTTATGTGGGCGGTGATCAGAATCGACATGGTGGTGGATCATCATCGGGTTCTCAATCCTCATCAATGCCAACCAACATGCTTAAGAAAGGCCAGTTCTACGGCGAAGAGCTTCTCTACCGGCAGTCATCTCCGTCGCAACTTCCTATCTCCCCACAAACCGTTAAATGCCATACAAAAGTCGAAGCTTTTGTTCTTATGGCCAAGGACTGGACAAGTATAGTCTCCAAATGCAGATCGTTGTTGCCGAACAACATGAACCACGACAACAACAACAACAACAACAACAACAACAACAACAACAACAACAACAACAACAATAATATGAACGTTACTCATTATGGACAGCCCTTACTGAGGGGCTTCGAGAGCTCTACGGCCTCGACACCAGGAGTCCGGTATTTCGAACCTGCTATGCGCCCCAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
Ion_trans

Protein Analysis

630

Amino Acids

72.15

Weight (kDa)

9.2

Isoelectric Point (pI)

42.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ion_trans PF00520 55 - 364 4.1e-08 Ion transport protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000119)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g10570 FvH4_4g36390 FvH4_7g31840 FvH4_7g31840 FvH4_7g31850 FvH4_7g31850 FvH4_7g31861 FvH4_7g31861 FvH4_7g31950
malus_domestica MD01G1054600.v1.1 MD01G1221100.v1.1 MD01G1221400.v1.1 MD01G1221600.v1.1 MD01G1221800.v1.1 MD01G1222000.v1.1 MD01G1222100.v1.1 MD07G1140800.v1.1 MD07G1291200.v1.1 MD07G1291400.v1.1 MD07G1291500.v1.1 MD07G1291700.v1.1 MD07G1292500.v1.1 MD07G1292700.v1.1 MD07G1292800.v1.1 MD07G1292900.v1.1 MD08G1241600.v1.1 MD11G1083800.v1.1 MD15G1431600.v1.1
prunus_persica Prupe.1G017800_v2.0.a1 Prupe.1G017800_v2.0.a1 Prupe.1G162800_v2.0.a1 Prupe.2G312500_v2.0.a1 Prupe.2G312500_v2.0.a1 Prupe.2G312700_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092800_v2.0.a1 Prupe.4G092800_v2.0.a1 Prupe.6G142800_v2.0.a1 Prupe.6G142800_v2.0.a1 Prupe.6G143000_v2.0.a1 Prupe.6G143100_v2.0.a1 Prupe.6G143100_v2.0.a1 Prupe.6G143100_v2.0.a1 Prupe.6G143100_v2.0.a1
pyrus_communis pycom01g23050 pycom01g23070 pycom01g23090 pycom01g23100 pycom01g23110 pycom01g23120 pycom01g23170 pycom07g26410 pycom07g26440 pycom07g26470 pycom07g26480 pycom07g26490 pycom07g26500 pycom07g26510 pycom07g26530 pycom07g26540 pycom07g26570 pycom07g26580 pycom07g26590 pycom08g20940
rosa_chinensis RchiOBHm_Chr1g0324571 RchiOBHm_Chr1g0336181 RchiOBHm_Chr1g0381061 RchiOBHm_Chr1g0381091 RchiOBHm_Chr1g0381121 RchiOBHm_Chr1g0381131 RchiOBHm_Chr1g0381141 RchiOBHm_Chr1g0381161 RchiOBHm_Chr1g0381211 RchiOBHm_Chr2g0110361 RchiOBHm_Chr2g0120231 RchiOBHm_Chr3g0486281 RchiOBHm_Chr3g0486311 RchiOBHm_Chr3g0486401 RchiOBHm_Chr4g0409511 RchiOBHm_Chr4g0409521 RchiOBHm_Chr5g0017271 RchiOBHm_Chr5g0048781 RchiOBHm_Chr5g0048811
rosa_laevigata RLG00000002401 RLG00000008559 RLG00000017798 RLG00000017799 RLG00000023082 RLG00000023088 RLG00000026230 RLG00000026238 RLG00000026240 RLG00000026241 RLG00000026242 RLG00000026243 RLG00000026244 RLG00000026246 RLG00000026247 RLG00000026248 RLG00000029876 RLG00000030189 RLG00000034579
rosa_multiflora Rmu_co8101374.1_g000001 Rmu_co8309149.1_g000001 Rmu_co8450063.1_g000001 Rmu_sc0000441.1_g000094 Rmu_sc0000479.1_g000005 Rmu_sc0000647.1_g000006 Rmu_sc0001393.1_g000031 Rmu_sc0001423.1_g000037 Rmu_sc0001616.1_g000015 Rmu_sc0001857.1_g000011 Rmu_sc0002183.1_g000013 Rmu_sc0002183.1_g000028 Rmu_sc0002183.1_g000030 Rmu_sc0002688.1_g000003 Rmu_sc0002688.1_g000020 Rmu_sc0003801.1_g000003 Rmu_sc0003801.1_g000004 Rmu_sc0004125.1_g000006 Rmu_sc0004494.1_g000005 Rmu_sc0004494.1_g000006 Rmu_sc0004494.1_g000008 Rmu_sc0004494.1_g000009 Rmu_sc0005842.1_g000004 Rmu_sc0006872.1_g000001 Rmu_sc0006872.1_g000005 Rmu_sc0006872.1_g000009 Rmu_sc0006872.1_g000010 Rmu_sc0008053.1_g000026
rosa_roxburghii Rroxscaffold_159G00432960 Rroxscaffold_1G00033060 Rroxscaffold_2G00123150 Rroxscaffold_2G00123170 Rroxscaffold_2G00133500 Rroxscaffold_2G00133550 Rroxscaffold_4G00278650 Rroxscaffold_4G00278750 Rroxscaffold_4G00278790 Rroxscaffold_4G00278800 Rroxscaffold_4G00278810 Rroxscaffold_4G00325390 Rroxscaffold_5G00354010 Rroxscaffold_6G00395890 Rroxscaffold_6G00395900 Rroxscaffold_6G00395920
rosa_rugosa Rorug01G0046900.1 Rorug01G0427000 Rorug01G0427100 Rorug01G0427200 Rorug01G0427300 Rorug01G0427400 Rorug01G0427500 Rorug01G0427500 Rorug01G0427600 Rorug01G0427600 Rorug01G0427700 Rorug01G0427700 Rorug01G0427800 Rorug01G0428700 Rorug02G0166400 Rorug02G0166500 Rorug02G0166500 Rorug02G0166600 Rorug02G0166600 Rorug02G0166700 Rorug02G0167300 Rorug02G0167300 Rorug02G0167300 Rorug03G0224600 Rorug03G0224600 Rorug04G0094400 Rorug05G0241100 Rorug05G0241200
rosa_samantha Rh1BG407700 Rh1BG407900 Rh1BG409000 Rh1CG064700 Rh1CG420900 Rh1CG421000 Rh1CG421200 Rh1CG421300 Rh1CG421800 Rh1CG421900 Rh1CG422000 Rh1CG422200 Rh1CG422300 Rh1CG423600 Rh1DG437600 Rh1DG438900 Rh1DG439000 Rh1DG440300 Rh2AG219100 Rh2CG220700 Rh2DG225300 Rh3AG274400 Rh3AG274600 Rh3CG308900 Rh3DG305800 Rh4AG156400 Rh5AG129000 Rh5AG320900 Rh5CG139600
rosa_wichuraiana Rw1G005300 Rw1G039280 Rw1G039300 Rw1G039310 Rw1G039320 Rw1G039330 Rw1G039340 Rw1G039360 Rw1G039390 Rw2G016910 Rw2G022800 Rw3G024330 Rw3G024340 Rw3G024350 Rw3G024400 Rw4G012810 Rw5G030120 Rw5G030280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1879
AccB7I CCANNNNNTGG 1 cut(s) 1671
AccII CGCG 1 cut(s) 890
AciI CCGC 4 cut(s) 74, 842, 890, 1473
AclI AACGTT 1 cut(s) 1791
AclWI GGATC 2 cut(s) 132, 1507
AcoI YGGCCR 1 cut(s) 1662
AcsI RAATTY 4 cut(s) 104, 330, 570, 946
AcuI CTGAAG 1 cut(s) 284
AcyI GRCGYC 1 cut(s) 893
AfaI GTAC 9 cut(s) 26, 100, 168, 660, 751, 982, 1265, 1317, 1385
AfiI CCNNNNNNNGG 5 cut(s) 80, 250, 775, 1671, 1818
AflII CTTAAG 1 cut(s) 1544
AgsI TTSAA 7 cut(s) 35, 55, 487, 628, 692, 878, 1351
AjnI CCWGG 2 cut(s) 79, 1849
AjuI GAANNNNNNNTTGG 2 cut(s) 608, 640
AluBI AGCT 7 cut(s) 89, 262, 389, 1398, 1573, 1649, 1833
AluI AGCT 7 cut(s) 89, 262, 389, 1398, 1573, 1649, 1833
Alw21I GWGCWC 1 cut(s) 1835
Alw26I GTCTC 3 cut(s) 692, 1410, 1690
AlwI GGATC 2 cut(s) 132, 1507
AoxI GGCC 7 cut(s) 113, 339, 612, 1373, 1552, 1662, 1839
ApoI RAATTY 4 cut(s) 104, 330, 570, 946
Asp700I GAANNNNTTC 3 cut(s) 1244, 1397, 1572
AspLEI GCGC 2 cut(s) 420, 1881
AspS9I GGNCC 3 cut(s) 113, 196, 612
AsuC2I CCSGG 1 cut(s) 378
AsuHPI GGTGA 4 cut(s) 84, 566, 928, 1487
AsuII TTCGAA 1 cut(s) 1866
AvaII GGWCC 1 cut(s) 196
AxyI CCTNAGG 1 cut(s) 1307
BalI TGGCCA 1 cut(s) 1664
BanII GRGCYC 1 cut(s) 1835
Bbv12I GWGCWC 1 cut(s) 1835
BccI CCATC 2 cut(s) 461, 1141
BceAI ACGGC 4 cut(s) 792, 829, 1579, 1854
BciT130I CCWGG 2 cut(s) 81, 1851
BclI TGATCA 1 cut(s) 1477
BcnI CCSGG 1 cut(s) 378
BcoDI GTCTC 3 cut(s) 692, 1410, 1690
BfaI CTAG 2 cut(s) 96, 800
BfoI RGCGCY 1 cut(s) 421
BfrI CTTAAG 1 cut(s) 1544
BfuAI ACCTGC 1 cut(s) 1879
BisI GCNGC 1 cut(s) 891
BlsI GCNGC 1 cut(s) 892
Bme1390I CCNGG 3 cut(s) 81, 378, 1851
Bme18I GGWCC 1 cut(s) 196
BmgT120I GGNCC 3 cut(s) 113, 196, 612
BmiI GGNNCC 2 cut(s) 198, 667
BmrFI CCNGG 3 cut(s) 81, 378, 1851
BplI GAGNNNNNCTC 2 cut(s) 1407, 1439
BpmI CTGGAG 1 cut(s) 102
Bpu14I TTCGAA 1 cut(s) 1866
BpuMI CCSGG 1 cut(s) 378
Bsa29I ATCGAT 1 cut(s) 225
BsaBI GATNNNNATC 2 cut(s) 1482, 1504
BsaHI GRCGYC 1 cut(s) 893
BsaJI CCNNGG 4 cut(s) 244, 557, 1259, 1665
BsaWI WCCGGW 1 cut(s) 1857
Bsc4I CCNNNNNNNGG 5 cut(s) 80, 250, 775, 1671, 1818
Bse118I RCCGGY 1 cut(s) 1581
Bse1I ACTGG 6 cut(s) 126, 198, 607, 1376, 1555, 1676
Bse21I CCTNAGG 1 cut(s) 1307
Bse3DI GCAATG 1 cut(s) 645
Bse8I GATNNNNATC 2 cut(s) 1482, 1504
BseBI CCWGG 2 cut(s) 81, 1851
BseCI ATCGAT 1 cut(s) 225
BseDI CCNNGG 4 cut(s) 244, 557, 1259, 1665
BseGI GGATG 4 cut(s) 453, 1152, 1342, 1437
BseJI GATNNNNATC 2 cut(s) 1482, 1504
BseLI CCNNNNNNNGG 5 cut(s) 80, 250, 775, 1671, 1818
BseMI GCAATG 1 cut(s) 645
BseMII CTCAG 2 cut(s) 1361, 1808
BseNI ACTGG 6 cut(s) 126, 198, 607, 1376, 1555, 1676
Bsh1236I CGCG 1 cut(s) 890
Bsh1285I CGRYCG 3 cut(s) 426, 769, 1429
BshFI GGCC 7 cut(s) 115, 341, 614, 1375, 1554, 1664, 1841
BshVI ATCGAT 1 cut(s) 225
BsiEI CGRYCG 3 cut(s) 426, 769, 1429
BsiHKAI GWGCWC 1 cut(s) 1835
BsiSI CCGG 3 cut(s) 378, 1582, 1858
BsiWI CGTACG 1 cut(s) 1383
BslFI GGGAC 2 cut(s) 798, 951
BslI CCNNNNNNNGG 5 cut(s) 80, 250, 775, 1671, 1818
BsmAI GTCTC 3 cut(s) 692, 1410, 1690
BsmFI GGGAC 2 cut(s) 798, 951
BsmI GAATGC 2 cut(s) 274, 623
BsnI GGCC 7 cut(s) 115, 341, 614, 1375, 1554, 1664, 1841
Bsp119I TTCGAA 1 cut(s) 1866
Bsp1286I GDGCHC 1 cut(s) 1835
BspACI CCGC 4 cut(s) 74, 842, 890, 1473
BspANI GGCC 7 cut(s) 115, 341, 614, 1375, 1554, 1664, 1841
BspCNI CTCAG 2 cut(s) 1362, 1809
BspDI ATCGAT 1 cut(s) 225
BspFNI CGCG 1 cut(s) 890
BspHI TCATGA 1 cut(s) 1296
BspLI GGNNCC 2 cut(s) 198, 667
BspMI ACCTGC 1 cut(s) 1879
BspPI GGATC 2 cut(s) 132, 1507
BspQI GCTCTTC 1 cut(s) 1563
BspT104I TTCGAA 1 cut(s) 1866
BspTI CTTAAG 1 cut(s) 1544
BsrDI GCAATG 1 cut(s) 645
BsrFI RCCGGY 1 cut(s) 1581
BsrI ACTGG 6 cut(s) 126, 198, 607, 1376, 1555, 1676
BssAI RCCGGY 1 cut(s) 1581
BssECI CCNNGG 4 cut(s) 244, 557, 1259, 1665
BssNI GRCGYC 1 cut(s) 893
BssT1I CCWWGG 3 cut(s) 244, 1259, 1665
Bst2UI CCWGG 2 cut(s) 81, 1851
Bst4CI ACNGT 5 cut(s) 150, 761, 770, 1315, 1624
Bst6I CTCTTC 4 cut(s) 209, 879, 1134, 1563
BstACI GRCGYC 1 cut(s) 893
BstAFI CTTAAG 1 cut(s) 1544
BstBI TTCGAA 1 cut(s) 1866
BstDEI CTNAG 3 cut(s) 1307, 1370, 1817
BstENI CCTNNNNNAGG 1 cut(s) 1816
BstF5I GGATG 4 cut(s) 453, 1152, 1342, 1437
BstFNI CGCG 1 cut(s) 890
BstH2I RGCGCY 1 cut(s) 421
BstHHI GCGC 2 cut(s) 420, 1881
BstMAI GTCTC 3 cut(s) 692, 1410, 1690
BstMCI CGRYCG 3 cut(s) 426, 769, 1429
BstMWI GCNNNNNNNGC 3 cut(s) 620, 960, 1830
BstNI CCWGG 2 cut(s) 81, 1851
BstNSI RCATGY 2 cut(s) 11, 1543
BstSCI CCNGG 3 cut(s) 79, 376, 1849
BstUI CGCG 1 cut(s) 890
Bsu15I ATCGAT 1 cut(s) 225
Bsu36I CCTNAGG 1 cut(s) 1307
BsuRI GGCC 7 cut(s) 115, 341, 614, 1375, 1554, 1664, 1841
BsuTUI ATCGAT 1 cut(s) 225
BtsCI GGATG 4 cut(s) 453, 1152, 1342, 1437
BtsIMutI CAGTG 2 cut(s) 191, 1383
BveI ACCTGC 1 cut(s) 1879
CciI TCATGA 1 cut(s) 1296
CfoI GCGC 2 cut(s) 420, 1881
Cfr10I RCCGGY 1 cut(s) 1581
Cfr13I GGNCC 3 cut(s) 113, 196, 612
ClaI ATCGAT 1 cut(s) 225
CseI GACGC 2 cut(s) 882, 1235
Csp6I GTAC 9 cut(s) 25, 99, 167, 659, 750, 981, 1264, 1316, 1384
CviQI GTAC 9 cut(s) 25, 99, 167, 659, 750, 981, 1264, 1316, 1384
DdeI CTNAG 3 cut(s) 1307, 1370, 1817
DraI TTTAAA 2 cut(s) 109, 1177
EaeI YGGCCR 1 cut(s) 1662
Eam1104I CTCTTC 4 cut(s) 209, 879, 1134, 1563
EarI CTCTTC 4 cut(s) 209, 879, 1134, 1563
Ecl136II GAGCTC 1 cut(s) 1833
Eco130I CCWWGG 3 cut(s) 244, 1259, 1665
Eco24I GRGCYC 1 cut(s) 1835
Eco47I GGWCC 1 cut(s) 196
Eco53kI GAGCTC 1 cut(s) 1833
Eco57I CTGAAG 1 cut(s) 284
Eco81I CCTNAGG 1 cut(s) 1307
EcoICRI GAGCTC 1 cut(s) 1833
EcoNI CCTNNNNNAGG 1 cut(s) 1816
EcoRI GAATTC 1 cut(s) 570
EcoRII CCWGG 2 cut(s) 79, 1849
EcoT14I CCWWGG 3 cut(s) 244, 1259, 1665
EcoT22I ATGCAT 1 cut(s) 9
EcoT38I GRGCYC 1 cut(s) 1835
ErhI CCWWGG 3 cut(s) 244, 1259, 1665
FaqI GGGAC 2 cut(s) 798, 951
FauI CCCGC 1 cut(s) 81
FauNDI CATATG 2 cut(s) 608, 985
FbaI TGATCA 1 cut(s) 1477
Fnu4HI GCNGC 1 cut(s) 891
FokI GGATG 4 cut(s) 440, 1159, 1349, 1444
FriOI GRGCYC 1 cut(s) 1835
Fsp4HI GCNGC 1 cut(s) 891
FspBI CTAG 2 cut(s) 96, 800
GlaI GCGC 2 cut(s) 419, 1880
GluI GCNGC 1 cut(s) 891
GsuI CTGGAG 1 cut(s) 102
HaeII RGCGCY 1 cut(s) 421
HaeIII GGCC 7 cut(s) 115, 341, 614, 1375, 1554, 1664, 1841
HapII CCGG 3 cut(s) 378, 1582, 1858
HgaI GACGC 2 cut(s) 882, 1235
HhaI GCGC 2 cut(s) 420, 1881
Hin1I GRCGYC 1 cut(s) 893
Hin6I GCGC 2 cut(s) 418, 1879
HinP1I GCGC 2 cut(s) 418, 1879
HincII GTYRAC 1 cut(s) 355
HindII GTYRAC 1 cut(s) 355
HindIII AAGCTT 1 cut(s) 1647
HinfI GANTC 5 cut(s) 806, 869, 1240, 1483, 1854
HpaII CCGG 3 cut(s) 378, 1582, 1858
HphI GGTGA 4 cut(s) 84, 566, 928, 1487
Hpy166II GTNNAC 4 cut(s) 27, 355, 481, 1266
Hpy188I TCNGA 7 cut(s) 558, 857, 972, 1096, 1371, 1431, 1482
Hpy188III TCNNGA 8 cut(s) 501, 800, 866, 882, 1189, 1297, 1424, 1828
Hpy8I GTNNAC 4 cut(s) 27, 355, 481, 1266
Hpy99I CGWCG 3 cut(s) 898, 1391, 1600
HpyAV CCTTC 2 cut(s) 548, 1442
HpyCH4III ACNGT 5 cut(s) 150, 761, 770, 1315, 1624
HpyCH4IV ACGT 4 cut(s) 1067, 1197, 1382, 1791
HpyF10VI GCNNNNNNNGC 3 cut(s) 620, 960, 1830
HpyF3I CTNAG 3 cut(s) 1307, 1370, 1817
HpySE526I ACGT 4 cut(s) 1067, 1197, 1382, 1791
Hsp92I GRCGYC 1 cut(s) 893
HspAI GCGC 2 cut(s) 418, 1879
Ksp22I TGATCA 1 cut(s) 1477
LguI GCTCTTC 1 cut(s) 1563
LmnI GCTCC 1 cut(s) 665
MaeI CTAG 2 cut(s) 96, 800
MaeII ACGT 4 cut(s) 1067, 1197, 1382, 1791
MaeIII GTNAC 5 cut(s) 189, 427, 761, 1378, 1792
MfeI CAATTG 1 cut(s) 1076
MhlI GDGCHC 1 cut(s) 1835
MlsI TGGCCA 1 cut(s) 1664
MluNI TGGCCA 1 cut(s) 1664
MlyI GAGTC 1 cut(s) 1863
MmeI TCCRAC 2 cut(s) 879, 1074
MnlI CCTC 8 cut(s) 210, 552, 592, 1090, 1365, 1531, 1812, 1852
Mox20I TGGCCA 1 cut(s) 1664
Mph1103I ATGCAT 1 cut(s) 9
MroXI GAANNNNTTC 3 cut(s) 1244, 1397, 1572
MscI TGGCCA 1 cut(s) 1664
MslI CAYNNNNRTG 2 cut(s) 988, 1494
Msp20I TGGCCA 1 cut(s) 1664
MspCI CTTAAG 1 cut(s) 1544
MspI CCGG 3 cut(s) 378, 1582, 1858
MspR9I CCNGG 3 cut(s) 81, 378, 1851
MunI CAATTG 1 cut(s) 1076
Mva1269I GAATGC 2 cut(s) 274, 623
MvaI CCWGG 2 cut(s) 81, 1851
MvnI CGCG 1 cut(s) 890
MwoI GCNNNNNNNGC 3 cut(s) 620, 960, 1830
NciI CCSGG 1 cut(s) 378
NdeI CATATG 2 cut(s) 608, 985
NlaIV GGNNCC 2 cut(s) 198, 667
NmuCI GTSAC 3 cut(s) 189, 761, 1378
NsiI ATGCAT 1 cut(s) 9
NspI RCATGY 2 cut(s) 11, 1543
NspV TTCGAA 1 cut(s) 1866
PagI TCATGA 1 cut(s) 1296
PciSI GCTCTTC 1 cut(s) 1563
PcsI WCGNNNNNNNCGW 2 cut(s) 419, 1706
PctI GAATGC 2 cut(s) 274, 623
PdmI GAANNNNTTC 3 cut(s) 1244, 1397, 1572
PfeI GAWTC 4 cut(s) 806, 869, 1240, 1483
Pfl23II CGTACG 1 cut(s) 1383
PflMI CCANNNNNTGG 1 cut(s) 1671
PkrI GCNGC 1 cut(s) 892
Ple19I CGATCG 2 cut(s) 426, 1429
PleI GAGTC 1 cut(s) 1862
PpsI GAGTC 1 cut(s) 1862
Psp124BI GAGCTC 1 cut(s) 1835
Psp1406I AACGTT 1 cut(s) 1791
Psp6I CCWGG 2 cut(s) 79, 1849
PspGI CCWGG 2 cut(s) 79, 1849
PspLI CGTACG 1 cut(s) 1383
PspN4I GGNNCC 2 cut(s) 198, 667
PspPI GGNCC 3 cut(s) 113, 196, 612
PvuI CGATCG 2 cut(s) 426, 1429
RsaI GTAC 9 cut(s) 26, 100, 168, 660, 751, 982, 1265, 1317, 1385
RsaNI GTAC 9 cut(s) 25, 99, 167, 659, 750, 981, 1264, 1316, 1384
RseI CAYNNNNRTG 2 cut(s) 988, 1494
SacI GAGCTC 1 cut(s) 1835
SapI GCTCTTC 1 cut(s) 1563
SatI GCNGC 1 cut(s) 891
Sau96I GGNCC 3 cut(s) 113, 196, 612
SchI GAGTC 1 cut(s) 1863
ScrFI CCNGG 3 cut(s) 81, 378, 1851
SduI GDGCHC 1 cut(s) 1835
SfuI TTCGAA 1 cut(s) 1866
SinI GGWCC 1 cut(s) 196
SmiMI CAYNNNNRTG 2 cut(s) 988, 1494
SmlI CTYRAG 1 cut(s) 1544
SmoI CTYRAG 1 cut(s) 1544
SsiI CCGC 4 cut(s) 74, 842, 890, 1473
SspI AATATT 3 cut(s) 162, 861, 1032
SspMI CTAG 2 cut(s) 96, 800
SstI GAGCTC 1 cut(s) 1835
StyD4I CCNGG 3 cut(s) 79, 376, 1849
StyI CCWWGG 3 cut(s) 244, 1259, 1665
TaaI ACNGT 5 cut(s) 150, 761, 770, 1315, 1624
TaiI ACGT 4 cut(s) 1070, 1200, 1385, 1794
TatI WGTACW 5 cut(s) 24, 98, 166, 658, 980
TauI GCSGC 1 cut(s) 893
TfiI GAWTC 4 cut(s) 806, 869, 1240, 1483
TscAI CASTG 2 cut(s) 198, 1383
TseFI GTSAC 3 cut(s) 189, 761, 1378
Tsp45I GTSAC 3 cut(s) 189, 761, 1378
TspGWI ACGGA 2 cut(s) 1460, 1584
TspRI CASTG 2 cut(s) 198, 1383
Van91I CCANNNNNTGG 1 cut(s) 1671
Vha464I CTTAAG 1 cut(s) 1544
VpaK11BI GGWCC 1 cut(s) 196
XagI CCTNNNNNAGG 1 cut(s) 1816
XapI RAATTY 4 cut(s) 104, 330, 570, 946
XbaI TCTAGA 1 cut(s) 799
XceI RCATGY 2 cut(s) 11, 1543
XmnI GAANNNNTTC 3 cut(s) 1244, 1397, 1572
XspI CTAG 2 cut(s) 96, 800
Zsp2I ATGCAT 1 cut(s) 9
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.