MD01G1221800.v1.1

Cyclic nucleotide-gated ion channel 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0000119
Physical Location & Seq
Reverse (-)
31420071 .. 31428547
8477 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1221800.v1.1.491

Sequence Viewer

Length: 2286 bp
ATGGCTGGAAACGTCAAGGGAGCAGCTTCAGATGTGGAGAACCAGGCAGCAGAGAAAACACACAGAAAGGATGGAGAACGTTCGAAATCAATGTGCAGTAGCGACCTTACCTTGATAAAGAAGATTTGTAATGTGCATGAGGGGAGTCACCTTTTAGTATGGAACAGAATATTTGTGATATCATGCGTATTTGCTGTCTCTCTGGATCCCTTCTTCTTTTACGTTGTGATCATTGATCAGGATAACAAGTGCCTTCAAATGGACAAAACGTTGAGTATTGTTGTTTGTCTCATGCGATCACTCACGGATGTCATTTTCCTGGTGCATTTTATATGTGAAATTTGTGACCATATTCAAAGTTCGAAACGAAATACCGTGAAAAAAGGACCCTCTTCAGAGGTCAGACAAACTGGGGGAAGTTCAGATAAGAAATCTAAAATCAGAAAGTTGATTGAGAAAATGGCTCAAAAGATGTCGTGGTTATCTGTCTCCATTGTAATAGACTTTTTCTCCTTTATTCCCCTACCACAACTGCTAGTAGTCATGTTTTACACAATGAAAGGCTCAGGATTTGTGGAACATAAAAAGGTTCTGAATGTTTGTCTTCTCTGTCAATATTTTCCGAGGATTTATCTAATTTACCTATCGGCCAAGGAATTCAAAATGACTAATGGGATATGGATTAAGGGTCTATTCAACTTGTTTCTCTATATTCTTGCCAGCCACATACTCGGAGGTTTTTGGTACTTTTTTTCTATTCAACGAGAGTTATCATGTTGGCACAAGGCCTGTGAAAATTATATTATAGATCTCAGGGGATGTGTGAACACTTTCTACTGCGATAGTCAGAGTACTACTGCTAGAAATATAACATTTCTTAACGAGCGTTGCCCGTTGGATACTCCAGATGGTGTTTTATCTCCGATTAACTTTGGAATATTTCTTGATTCCCTTAAGAATCAAAACACGGAGCATATAAAGTTCGGAAAGAAGTTCTTTTACTCTTTCTGGTGGGGCTTGCGAAATCTAAGTAATTTTGGGACTAATCTGACAACAAGTACATATGTGTGGGAAAATTTGTTTGCGATTCTCATTTCTGTCGTTGGCTTGCTACTGTTTTTATATCTCATTGGAAACGTGCAGACTTTTATGCAGATGGAAGCTACCAAAAAAGAGGAGATGAGACAAAAGATTAAGATGAAGAGGCTAGACGTAGACACCTGGATGTCCAGAAATGAACTCCCTGAAGAAATAAAGAAAGAAATCTTGAGTAGCATAAAGCAAACATTGAAACAAAAAACATTGAAACAACACATTGACGCTGATCTCCATAGTTTTCTGTTCTTCATTCTTCCCGGGAAAACCAGAACATCTCTGAAGCGTTGTCTTTGCATGAAAACACTTAAGAAAGTAAAAATGCTTCAAGGCATGGATGACAAAGTGTTGACTTTGATGTGCGACTATCTGAAGCCAGTGACGTACATTGAGAACAGCAACATTTTTCGAATGGGAGATCCATTCGACTGCGTGCTAATCATTATGGAAGGGACAGTGTGGACTTACGCGTCGAGTGATAGTCAAGCTGGGCGAGGAATCTCATCAATGGGCACCAAGCCCCTCCGGAAAGGTGACTTTTACGGGGAAGAGCTTTTCGATTGGGCACCAGGCAGTTTCACCGAACTTCCAGTCTCTAGCAAACATGTCAAAACTCGGGTAAAAGTAGATGCATTTCTGCTCACGGCCCATGACTTGGATAGTGTAGTTTCCAAATACCGGCTACAGTGGAAGGAAGGCAAGAAGGGTACTCCTGATGAGAAGGTCGTGGCAGTTTCTACTATAGCAACAGCATACCGTGACTACCGTCATCATCGTCGTCTCCAAGCAATCACCAAAGTAAAAAAGCTTCAAGGCATGGATGACAAAGTGTTGACGTCAATCTGCAACTATCTGAAGCCAGTGACGTACAATGAGAACAGCTTCGTTTTTCGAATGGGAGATCCAGTCGACTGCATACTGTTCATTATCGAAGGGACAGTGTGGACCTACGCGTCGAGTGATAGTCAAGATGGGCAAGGAATCTCATCAATGGCCACCAAGACCCTCGGGAAAGGTGACTTTTACGGGGAAGAGCTCCTCGATTGCGCATCAGACAGTTTCACCGAACTTCCAGTCTCCAGCAAATATGTCGTGTGTCAGACAAAAGTAGAAGCATTTGTGCTCATGGCCAACGACTTGAAAACTGTAGTTTCCAAATACCCGCTACAGTGGGAGAAAAACGAGAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
Ion_trans cNMP_binding

Protein Analysis

762

Amino Acids

87.09

Weight (kDa)

8.95

Isoelectric Point (pI)

40.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
cNMP_binding PF00027 653 - 751 1.7e-06 Cyclic nucleotide-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000119)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g10570 FvH4_4g36390 FvH4_7g31840 FvH4_7g31840 FvH4_7g31850 FvH4_7g31850 FvH4_7g31861 FvH4_7g31861 FvH4_7g31950
malus_domestica MD01G1054600.v1.1 MD01G1221100.v1.1 MD01G1221400.v1.1 MD01G1221600.v1.1 MD01G1221800.v1.1 MD01G1222000.v1.1 MD01G1222100.v1.1 MD07G1140800.v1.1 MD07G1291200.v1.1 MD07G1291400.v1.1 MD07G1291500.v1.1 MD07G1291700.v1.1 MD07G1292500.v1.1 MD07G1292700.v1.1 MD07G1292800.v1.1 MD07G1292900.v1.1 MD08G1241600.v1.1 MD11G1083800.v1.1 MD15G1431600.v1.1
prunus_persica Prupe.1G017800_v2.0.a1 Prupe.1G017800_v2.0.a1 Prupe.1G162800_v2.0.a1 Prupe.2G312500_v2.0.a1 Prupe.2G312500_v2.0.a1 Prupe.2G312700_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092800_v2.0.a1 Prupe.4G092800_v2.0.a1 Prupe.6G142800_v2.0.a1 Prupe.6G142800_v2.0.a1 Prupe.6G143000_v2.0.a1 Prupe.6G143100_v2.0.a1 Prupe.6G143100_v2.0.a1 Prupe.6G143100_v2.0.a1 Prupe.6G143100_v2.0.a1
pyrus_communis pycom01g23050 pycom01g23070 pycom01g23090 pycom01g23100 pycom01g23110 pycom01g23120 pycom01g23170 pycom07g26410 pycom07g26440 pycom07g26470 pycom07g26480 pycom07g26490 pycom07g26500 pycom07g26510 pycom07g26530 pycom07g26540 pycom07g26570 pycom07g26580 pycom07g26590 pycom08g20940
rosa_chinensis RchiOBHm_Chr1g0324571 RchiOBHm_Chr1g0336181 RchiOBHm_Chr1g0381061 RchiOBHm_Chr1g0381091 RchiOBHm_Chr1g0381121 RchiOBHm_Chr1g0381131 RchiOBHm_Chr1g0381141 RchiOBHm_Chr1g0381161 RchiOBHm_Chr1g0381211 RchiOBHm_Chr2g0110361 RchiOBHm_Chr2g0120231 RchiOBHm_Chr3g0486281 RchiOBHm_Chr3g0486311 RchiOBHm_Chr3g0486401 RchiOBHm_Chr4g0409511 RchiOBHm_Chr4g0409521 RchiOBHm_Chr5g0017271 RchiOBHm_Chr5g0048781 RchiOBHm_Chr5g0048811
rosa_laevigata RLG00000002401 RLG00000008559 RLG00000017798 RLG00000017799 RLG00000023082 RLG00000023088 RLG00000026230 RLG00000026238 RLG00000026240 RLG00000026241 RLG00000026242 RLG00000026243 RLG00000026244 RLG00000026246 RLG00000026247 RLG00000026248 RLG00000029876 RLG00000030189 RLG00000034579
rosa_multiflora Rmu_co8101374.1_g000001 Rmu_co8309149.1_g000001 Rmu_co8450063.1_g000001 Rmu_sc0000441.1_g000094 Rmu_sc0000479.1_g000005 Rmu_sc0000647.1_g000006 Rmu_sc0001393.1_g000031 Rmu_sc0001423.1_g000037 Rmu_sc0001616.1_g000015 Rmu_sc0001857.1_g000011 Rmu_sc0002183.1_g000013 Rmu_sc0002183.1_g000028 Rmu_sc0002183.1_g000030 Rmu_sc0002688.1_g000003 Rmu_sc0002688.1_g000020 Rmu_sc0003801.1_g000003 Rmu_sc0003801.1_g000004 Rmu_sc0004125.1_g000006 Rmu_sc0004494.1_g000005 Rmu_sc0004494.1_g000006 Rmu_sc0004494.1_g000008 Rmu_sc0004494.1_g000009 Rmu_sc0005842.1_g000004 Rmu_sc0006872.1_g000001 Rmu_sc0006872.1_g000005 Rmu_sc0006872.1_g000009 Rmu_sc0006872.1_g000010 Rmu_sc0008053.1_g000026
rosa_roxburghii Rroxscaffold_159G00432960 Rroxscaffold_1G00033060 Rroxscaffold_2G00123150 Rroxscaffold_2G00123170 Rroxscaffold_2G00133500 Rroxscaffold_2G00133550 Rroxscaffold_4G00278650 Rroxscaffold_4G00278750 Rroxscaffold_4G00278790 Rroxscaffold_4G00278800 Rroxscaffold_4G00278810 Rroxscaffold_4G00325390 Rroxscaffold_5G00354010 Rroxscaffold_6G00395890 Rroxscaffold_6G00395900 Rroxscaffold_6G00395920
rosa_rugosa Rorug01G0046900.1 Rorug01G0427000 Rorug01G0427100 Rorug01G0427200 Rorug01G0427300 Rorug01G0427400 Rorug01G0427500 Rorug01G0427500 Rorug01G0427600 Rorug01G0427600 Rorug01G0427700 Rorug01G0427700 Rorug01G0427800 Rorug01G0428700 Rorug02G0166400 Rorug02G0166500 Rorug02G0166500 Rorug02G0166600 Rorug02G0166600 Rorug02G0166700 Rorug02G0167300 Rorug02G0167300 Rorug02G0167300 Rorug03G0224600 Rorug03G0224600 Rorug04G0094400 Rorug05G0241100 Rorug05G0241200
rosa_samantha Rh1BG407700 Rh1BG407900 Rh1BG409000 Rh1CG064700 Rh1CG420900 Rh1CG421000 Rh1CG421200 Rh1CG421300 Rh1CG421800 Rh1CG421900 Rh1CG422000 Rh1CG422200 Rh1CG422300 Rh1CG423600 Rh1DG437600 Rh1DG438900 Rh1DG439000 Rh1DG440300 Rh2AG219100 Rh2CG220700 Rh2DG225300 Rh3AG274400 Rh3AG274600 Rh3CG308900 Rh3DG305800 Rh4AG156400 Rh5AG129000 Rh5AG320900 Rh5CG139600
rosa_wichuraiana Rw1G005300 Rw1G039280 Rw1G039300 Rw1G039310 Rw1G039320 Rw1G039330 Rw1G039340 Rw1G039360 Rw1G039390 Rw2G016910 Rw2G022800 Rw3G024330 Rw3G024340 Rw3G024350 Rw3G024400 Rw4G012810 Rw5G030120 Rw5G030280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 1564, 2047
AatII GACGTC 1 cut(s) 1934
Acc16I TGCGCA 1 cut(s) 2143
AccB1I GGYRCC 2 cut(s) 1607, 1660
AccB7I CCANNNNNTGG 1 cut(s) 1750
AccI GTMKAC 2 cut(s) 1215, 2004
AccII CGCG 2 cut(s) 1565, 2048
AccIII TCCGGA 1 cut(s) 1620
AciI CCGC 1 cut(s) 2258
AclI AACGTT 2 cut(s) 79, 269
AclWI GGATC 4 cut(s) 200, 213, 1508, 1991
AcoI YGGCCR 3 cut(s) 648, 2088, 2223
AcsI RAATTY 3 cut(s) 339, 656, 1075
AcuI CTGAAG 6 cut(s) 12, 378, 1266, 1397, 1487, 1970
AcyI GRCGYC 1 cut(s) 1931
AfaI GTAC 6 cut(s) 746, 853, 1060, 1481, 1804, 1964
AfiI CCNNNNNNNGG 3 cut(s) 259, 1750, 1773
AflII CTTAAG 2 cut(s) 953, 1403
AflIII ACRYGT 3 cut(s) 1563, 1699, 2046
AjnI CCWGG 4 cut(s) 42, 318, 1220, 1663
AluBI AGCT 7 cut(s) 26, 1163, 1583, 1648, 1903, 1977, 2131
AluI AGCT 7 cut(s) 26, 1163, 1583, 1648, 1903, 1977, 2131
Alw21I GWGCWC 2 cut(s) 2133, 2220
Alw26I GTCTC 7 cut(s) 202, 293, 493, 1177, 1693, 1880, 2176
AlwI GGATC 4 cut(s) 200, 213, 1508, 1991
Ama87I CYCGRG 3 cut(s) 1355, 1710, 2102
Aor13HI TCCGGA 1 cut(s) 1620
AoxI GGCC 5 cut(s) 648, 786, 1740, 2088, 2223
ApeKI GCWGC 2 cut(s) 23, 47
ApoI RAATTY 3 cut(s) 339, 656, 1075
ArsI GACNNNNNNTTYG 4 cut(s) 1671, 1703, 2154, 2186
Asp700I GAANNNNTTC 2 cut(s) 830, 1976
AspLEI GCGC 1 cut(s) 2144
AspS9I GGNCC 3 cut(s) 386, 1741, 2040
AsuC2I CCSGG 2 cut(s) 1356, 1357
AsuHPI GGTGA 6 cut(s) 140, 1640, 1666, 1879, 2123, 2149
AsuII TTCGAA 4 cut(s) 83, 362, 1504, 1987
AvaI CYCGRG 3 cut(s) 1355, 1710, 2102
AvaII GGWCC 2 cut(s) 386, 2040
BaeGI GKGCMC 2 cut(s) 1610, 1663
BalI TGGCCA 2 cut(s) 2090, 2225
BamHI GGATCC 1 cut(s) 205
BanI GGYRCC 2 cut(s) 1607, 1660
BanII GRGCYC 1 cut(s) 2133
BarI GAAGNNNNNNTAC 6 cut(s) 983, 1015, 1404, 1436, 1887, 1919
BbsI GAAGAC 1 cut(s) 596
Bbv12I GWGCWC 2 cut(s) 2133, 2220
BbvI GCAGC 2 cut(s) 35, 59
BccI CCATC 4 cut(s) 65, 902, 1150, 2060
BceAI ACGGC 1 cut(s) 1755
BcgI CGANNNNNNTGC 2 cut(s) 2167, 2201
BciT130I CCWGG 4 cut(s) 44, 320, 1222, 1665
BciVI GTATCC 1 cut(s) 892
BclI TGATCA 2 cut(s) 228, 235
BcnI CCSGG 2 cut(s) 1356, 1357
BcoDI GTCTC 7 cut(s) 202, 293, 493, 1177, 1693, 1880, 2176
BfaI CTAG 4 cut(s) 536, 861, 1208, 1692
BfmI CTRYAG 4 cut(s) 1778, 1836, 2241, 2261
BfrI CTTAAG 2 cut(s) 953, 1403
BfuI GTATCC 1 cut(s) 892
BglII AGATCT 1 cut(s) 808
BisI GCNGC 2 cut(s) 24, 48
BlsI GCNGC 2 cut(s) 25, 49
BmcAI AGTACT 1 cut(s) 853
Bme1390I CCNGG 6 cut(s) 44, 320, 1222, 1356, 1357, 1665
Bme18I GGWCC 2 cut(s) 386, 2040
BmeT110I CYCGRG 3 cut(s) 1355, 1710, 2102
BmgT120I GGNCC 3 cut(s) 386, 1741, 2040
BmiI GGNNCC 4 cut(s) 207, 388, 1609, 1662
BmrFI CCNGG 6 cut(s) 44, 320, 1222, 1356, 1357, 1665
BmrI ACTGGG 1 cut(s) 420
BmsI GCATC 2 cut(s) 1714, 2153
BmuI ACTGGG 1 cut(s) 420
BoxI GACNNNNGTC 1 cut(s) 1860
BpiI GAAGAC 1 cut(s) 596
BpmI CTGGAG 2 cut(s) 888, 2158
Bpu10I CCTNAGC 1 cut(s) 565
Bpu14I TTCGAA 4 cut(s) 83, 362, 1504, 1987
BpuEI CTTGAG 1 cut(s) 1288
BpuMI CCSGG 2 cut(s) 1356, 1357
BsaHI GRCGYC 1 cut(s) 1931
BsaJI CCNNGG 4 cut(s) 623, 651, 1355, 2101
BsaWI WCCGGW 1 cut(s) 1620
BsaXI ACNNNNNCTCC 4 cut(s) 494, 524, 1986, 2016
Bsc4I CCNNNNNNNGG 3 cut(s) 259, 1750, 1773
Bse118I RCCGGY 1 cut(s) 1773
Bse1I ACTGG 6 cut(s) 415, 1472, 1685, 1955, 2000, 2168
BseAI TCCGGA 1 cut(s) 1620
BseBI CCWGG 4 cut(s) 44, 320, 1222, 1665
BseDI CCNNGG 4 cut(s) 623, 651, 1355, 2101
BseGI GGATG 6 cut(s) 76, 313, 824, 1230, 1438, 1921
BseLI CCNNNNNNNGG 3 cut(s) 259, 1750, 1773
BseMII CTCAG 2 cut(s) 579, 826
BseNI ACTGG 6 cut(s) 415, 1472, 1685, 1955, 2000, 2168
BseRI GAGGAG 2 cut(s) 1190, 2123
BseSI GKGCMC 2 cut(s) 1610, 1663
BseXI GCAGC 2 cut(s) 35, 59
BseYI CCCAGC 1 cut(s) 1583
BsgI GTGCAG 2 cut(s) 115, 1160
Bsh1236I CGCG 2 cut(s) 1565, 2048
BshFI GGCC 5 cut(s) 650, 788, 1742, 2090, 2225
BshNI GGYRCC 2 cut(s) 1607, 1660
BsiHKAI GWGCWC 2 cut(s) 2133, 2220
BsiHKCI CYCGRG 3 cut(s) 1355, 1710, 2102
BsiSI CCGG 3 cut(s) 1356, 1621, 1774
BslFI GGGAC 3 cut(s) 1054, 1561, 2044
BslI CCNNNNNNNGG 3 cut(s) 259, 1750, 1773
BsmAI GTCTC 7 cut(s) 202, 293, 493, 1177, 1693, 1880, 2176
BsmBI CGTCTC 1 cut(s) 1880
BsmFI GGGAC 3 cut(s) 1054, 1561, 2044
BsnI GGCC 5 cut(s) 650, 788, 1742, 2090, 2225
BsoBI CYCGRG 3 cut(s) 1355, 1710, 2102
Bsp119I TTCGAA 4 cut(s) 83, 362, 1504, 1987
Bsp1286I GDGCHC 4 cut(s) 1610, 1663, 2133, 2220
Bsp13I TCCGGA 1 cut(s) 1620
Bsp143I GATC 8 cut(s) 205, 228, 235, 296, 808, 1324, 1513, 1996
BspACI CCGC 1 cut(s) 2258
BspANI GGCC 5 cut(s) 650, 788, 1742, 2090, 2225
BspCNI CTCAG 2 cut(s) 578, 825
BspEI TCCGGA 1 cut(s) 1620
BspFNI CGCG 2 cut(s) 1565, 2048
BspLI GGNNCC 4 cut(s) 207, 388, 1609, 1662
BspPI GGATC 4 cut(s) 200, 213, 1508, 1991
BspQI GCTCTTC 2 cut(s) 1638, 2121
BspT104I TTCGAA 4 cut(s) 83, 362, 1504, 1987
BspT107I GGYRCC 2 cut(s) 1607, 1660
BspTI CTTAAG 2 cut(s) 953, 1403
BsrFI RCCGGY 1 cut(s) 1773
BsrI ACTGG 6 cut(s) 415, 1472, 1685, 1955, 2000, 2168
BssAI RCCGGY 1 cut(s) 1773
BssECI CCNNGG 4 cut(s) 623, 651, 1355, 2101
BssMI GATC 8 cut(s) 205, 228, 235, 296, 808, 1324, 1513, 1996
BssNI GRCGYC 1 cut(s) 1931
BssT1I CCWWGG 1 cut(s) 651
Bst2UI CCWGG 4 cut(s) 44, 320, 1222, 1665
Bst6I CTCTTC 4 cut(s) 397, 1196, 1638, 2121
BstACI GRCGYC 1 cut(s) 1931
BstAFI CTTAAG 2 cut(s) 953, 1403
BstBI TTCGAA 4 cut(s) 83, 362, 1504, 1987
BstC8I GCNNGC 4 cut(s) 721, 1019, 1109, 1529
BstDEI CTNAG 3 cut(s) 565, 812, 1028
BstF5I GGATG 6 cut(s) 76, 313, 824, 1230, 1438, 1921
BstFNI CGCG 2 cut(s) 1565, 2048
BstHHI GCGC 1 cut(s) 2144
BstKTI GATC 8 cut(s) 208, 231, 238, 299, 811, 1327, 1516, 1999
BstMAI GTCTC 7 cut(s) 202, 293, 493, 1177, 1693, 1880, 2176
BstMBI GATC 8 cut(s) 205, 228, 235, 296, 808, 1324, 1513, 1996
BstNI CCWGG 4 cut(s) 44, 320, 1222, 1665
BstNSI RCATGY 1 cut(s) 1703
BstPAI GACNNNNGTC 1 cut(s) 1860
BstSCI CCNGG 6 cut(s) 42, 318, 1220, 1354, 1355, 1663
BstSFI CTRYAG 4 cut(s) 1778, 1836, 2241, 2261
BstSLI GKGCMC 2 cut(s) 1610, 1663
BstUI CGCG 2 cut(s) 1565, 2048
BstV1I GCAGC 2 cut(s) 35, 59
BstV2I GAAGAC 1 cut(s) 596
BstX2I RGATCY 4 cut(s) 205, 808, 1513, 1996
BstYI RGATCY 4 cut(s) 205, 808, 1513, 1996
BsuI GTATCC 1 cut(s) 892
BsuRI GGCC 5 cut(s) 650, 788, 1742, 2090, 2225
BtsCI GGATG 6 cut(s) 76, 313, 824, 1230, 1438, 1921
BtsIMutI CAGTG 6 cut(s) 1479, 1557, 1787, 1962, 2040, 2270
Cac8I GCNNGC 4 cut(s) 721, 1019, 1109, 1529
CfoI GCGC 1 cut(s) 2144
Cfr10I RCCGGY 1 cut(s) 1773
Cfr13I GGNCC 3 cut(s) 386, 1741, 2040
Cfr9I CCCGGG 1 cut(s) 1355
CseI GACGC 3 cut(s) 1328, 1554, 2037
Csp6I GTAC 6 cut(s) 745, 852, 1059, 1480, 1803, 1963
CviQI GTAC 6 cut(s) 745, 852, 1059, 1480, 1803, 1963
DdeI CTNAG 3 cut(s) 565, 812, 1028
DpnI GATC 8 cut(s) 207, 230, 237, 298, 810, 1326, 1515, 1998
DpnII GATC 8 cut(s) 205, 228, 235, 296, 808, 1324, 1513, 1996
DrdI GACNNNNNNGTC 2 cut(s) 1564, 2047
DseDI GACNNNNNNGTC 2 cut(s) 1564, 2047
EaeI YGGCCR 3 cut(s) 648, 2088, 2223
Eam1104I CTCTTC 4 cut(s) 397, 1196, 1638, 2121
EarI CTCTTC 4 cut(s) 397, 1196, 1638, 2121
Ecl136II GAGCTC 1 cut(s) 2131
Eco130I CCWWGG 1 cut(s) 651
Eco147I AGGCCT 1 cut(s) 788
Eco24I GRGCYC 1 cut(s) 2133
Eco32I GATATC 1 cut(s) 180
Eco47I GGWCC 2 cut(s) 386, 2040
Eco53kI GAGCTC 1 cut(s) 2131
Eco57I CTGAAG 6 cut(s) 12, 378, 1266, 1397, 1487, 1970
Eco88I CYCGRG 3 cut(s) 1355, 1710, 2102
EcoICRI GAGCTC 1 cut(s) 2131
EcoO109I RGGNCCY 1 cut(s) 386
EcoRI GAATTC 1 cut(s) 656
EcoRII CCWGG 4 cut(s) 42, 318, 1220, 1663
EcoRV GATATC 1 cut(s) 180
EcoT14I CCWWGG 1 cut(s) 651
EcoT22I ATGCAT 1 cut(s) 1729
EcoT38I GRGCYC 1 cut(s) 2133
ErhI CCWWGG 1 cut(s) 651
Esp3I CGTCTC 1 cut(s) 1880
FalI AAGNNNNNCTT 2 cut(s) 980, 1012
FaqI GGGAC 3 cut(s) 1054, 1561, 2044
FauI CCCGC 1 cut(s) 2265
FauNDI CATATG 1 cut(s) 1063
FbaI TGATCA 2 cut(s) 228, 235
FblI GTMKAC 2 cut(s) 1215, 2004
Fnu4HI GCNGC 2 cut(s) 24, 48
FokI GGATG 6 cut(s) 83, 320, 831, 1237, 1445, 1928
FriOI GRGCYC 1 cut(s) 2133
Fsp4HI GCNGC 2 cut(s) 24, 48
FspBI CTAG 4 cut(s) 536, 861, 1208, 1692
FspI TGCGCA 1 cut(s) 2143
GlaI GCGC 1 cut(s) 2143
GluI GCNGC 2 cut(s) 24, 48
GsaI CCCAGC 1 cut(s) 1587
GsuI CTGGAG 2 cut(s) 888, 2158
HaeIII GGCC 5 cut(s) 650, 788, 1742, 2090, 2225
HapII CCGG 3 cut(s) 1356, 1621, 1774
HgaI GACGC 3 cut(s) 1328, 1554, 2037
HhaI GCGC 1 cut(s) 2144
Hin1I GRCGYC 1 cut(s) 1931
Hin6I GCGC 1 cut(s) 2142
HinP1I GCGC 1 cut(s) 2142
HincII GTYRAC 3 cut(s) 1446, 1929, 2005
HindII GTYRAC 3 cut(s) 1446, 1929, 2005
HindIII AAGCTT 1 cut(s) 1901
HinfI GANTC 6 cut(s) 145, 947, 958, 1087, 1593, 2076
HpaII CCGG 3 cut(s) 1356, 1621, 1774
HphI GGTGA 6 cut(s) 140, 1640, 1666, 1879, 2123, 2149
Hpy166II GTNNAC 7 cut(s) 826, 1216, 1446, 1557, 1929, 2005, 2040
Hpy8I GTNNAC 7 cut(s) 826, 1216, 1446, 1557, 1929, 2005, 2040
Hpy99I CGWCG 3 cut(s) 1570, 1875, 2053
HpyAV CCTTC 8 cut(s) 220, 263, 1538, 1780, 1784, 1792, 1810, 2021
HpyCH4IV ACGT 9 cut(s) 12, 79, 222, 269, 1137, 1212, 1478, 1931, 1961
HpyCH4V TGCA 9 cut(s) 96, 136, 325, 1141, 1153, 1392, 1727, 1941, 2010
HpyF3I CTNAG 3 cut(s) 565, 812, 1028
HpySE526I ACGT 9 cut(s) 12, 79, 222, 269, 1137, 1212, 1478, 1931, 1961
Hsp92I GRCGYC 1 cut(s) 1931
HspAI GCGC 1 cut(s) 2142
Kpn2I TCCGGA 1 cut(s) 1620
Ksp22I TGATCA 2 cut(s) 228, 235
Kzo9I GATC 8 cut(s) 205, 228, 235, 296, 808, 1324, 1513, 1996
LguI GCTCTTC 2 cut(s) 1638, 2121
LmnI GCTCC 3 cut(s) 20, 970, 2136
Lsp1109I GCAGC 2 cut(s) 35, 59
LweI GCATC 2 cut(s) 1714, 2153
MaeI CTAG 4 cut(s) 536, 861, 1208, 1692
MaeII ACGT 9 cut(s) 12, 79, 222, 269, 1137, 1212, 1478, 1931, 1961
MaeIII GTNAC 7 cut(s) 146, 344, 1474, 1628, 1853, 1957, 2111
MalI GATC 8 cut(s) 207, 230, 237, 298, 810, 1326, 1515, 1998
MboI GATC 8 cut(s) 205, 228, 235, 296, 808, 1324, 1513, 1996
MflI RGATCY 4 cut(s) 205, 808, 1513, 1996
MhlI GDGCHC 4 cut(s) 1610, 1663, 2133, 2220
MlsI TGGCCA 2 cut(s) 2090, 2225
MluCI AATT 6 cut(s) 339, 636, 656, 796, 1033, 1075
MluI ACGCGT 2 cut(s) 1563, 2046
MluNI TGGCCA 2 cut(s) 2090, 2225
MlyI GAGTC 1 cut(s) 154
MmeI TCCRAC 1 cut(s) 876
Mox20I TGGCCA 2 cut(s) 2090, 2225
Mph1103I ATGCAT 1 cut(s) 1729
MroI TCCGGA 1 cut(s) 1620
MroXI GAANNNNTTC 2 cut(s) 830, 1976
MscI TGGCCA 2 cut(s) 2090, 2225
MseI TTAA 6 cut(s) 684, 879, 927, 954, 1194, 1404
MslI CAYNNNNRTG 2 cut(s) 1066, 1223
Msp20I TGGCCA 2 cut(s) 2090, 2225
MspCI CTTAAG 2 cut(s) 953, 1403
MspI CCGG 3 cut(s) 1356, 1621, 1774
MspR9I CCNGG 6 cut(s) 44, 320, 1222, 1356, 1357, 1665
MvaI CCWGG 4 cut(s) 44, 320, 1222, 1665
MvnI CGCG 2 cut(s) 1565, 2048
NciI CCSGG 2 cut(s) 1356, 1357
NdeI CATATG 1 cut(s) 1063
NdeII GATC 8 cut(s) 205, 228, 235, 296, 808, 1324, 1513, 1996
NlaIV GGNNCC 4 cut(s) 207, 388, 1609, 1662
NmuCI GTSAC 7 cut(s) 146, 344, 1474, 1628, 1853, 1957, 2111
NsbI TGCGCA 1 cut(s) 2143
NsiI ATGCAT 1 cut(s) 1729
NspI RCATGY 1 cut(s) 1703
NspV TTCGAA 4 cut(s) 83, 362, 1504, 1987
PceI AGGCCT 1 cut(s) 788
PciI ACATGT 1 cut(s) 1699
PciSI GCTCTTC 2 cut(s) 1638, 2121
PdmI GAANNNNTTC 2 cut(s) 830, 1976
PfeI GAWTC 5 cut(s) 947, 958, 1087, 1593, 2076
PflMI CCANNNNNTGG 1 cut(s) 1750
PkrI GCNGC 2 cut(s) 25, 49
PleI GAGTC 1 cut(s) 153
PpsI GAGTC 1 cut(s) 153
PpuMI RGGWCCY 1 cut(s) 386
PscI ACATGT 1 cut(s) 1699
PshAI GACNNNNGTC 1 cut(s) 1860
Psp124BI GAGCTC 1 cut(s) 2133
Psp1406I AACGTT 2 cut(s) 79, 269
Psp5II RGGWCCY 1 cut(s) 386
Psp6I CCWGG 4 cut(s) 42, 318, 1220, 1663
PspFI CCCAGC 1 cut(s) 1583
PspGI CCWGG 4 cut(s) 42, 318, 1220, 1663
PspN4I GGNNCC 4 cut(s) 207, 388, 1609, 1662
PspPI GGNCC 3 cut(s) 386, 1741, 2040
PspPPI RGGWCCY 1 cut(s) 386
PsrI GAACNNNNNNTAC 2 cut(s) 818, 850
PsuI RGATCY 4 cut(s) 205, 808, 1513, 1996
RsaI GTAC 6 cut(s) 746, 853, 1060, 1481, 1804, 1964
RsaNI GTAC 6 cut(s) 745, 852, 1059, 1480, 1803, 1963
RseI CAYNNNNRTG 2 cut(s) 1066, 1223
SacI GAGCTC 1 cut(s) 2133
SalI GTCGAC 1 cut(s) 2003
SapI GCTCTTC 2 cut(s) 1638, 2121
SaqAI TTAA 6 cut(s) 684, 879, 927, 954, 1194, 1404
SatI GCNGC 2 cut(s) 24, 48
Sau3AI GATC 8 cut(s) 205, 228, 235, 296, 808, 1324, 1513, 1996
Sau96I GGNCC 3 cut(s) 386, 1741, 2040
ScaI AGTACT 1 cut(s) 853
SchI GAGTC 1 cut(s) 154
ScrFI CCNGG 6 cut(s) 44, 320, 1222, 1356, 1357, 1665
SduI GDGCHC 4 cut(s) 1610, 1663, 2133, 2220
SfaNI GCATC 2 cut(s) 1714, 2153
SfcI CTRYAG 4 cut(s) 1778, 1836, 2241, 2261
SfuI TTCGAA 4 cut(s) 83, 362, 1504, 1987
SinI GGWCC 2 cut(s) 386, 2040
SmaI CCCGGG 1 cut(s) 1357
SmiMI CAYNNNNRTG 2 cut(s) 1066, 1223
SmlI CTYRAG 3 cut(s) 953, 1267, 1403
SmoI CTYRAG 3 cut(s) 953, 1267, 1403
Sse9I AATT 6 cut(s) 339, 636, 656, 796, 1033, 1075
SseBI AGGCCT 1 cut(s) 788
SsiI CCGC 1 cut(s) 2258
SspI AATATT 3 cut(s) 171, 617, 939
SspMI CTAG 4 cut(s) 536, 861, 1208, 1692
SstI GAGCTC 1 cut(s) 2133
StuI AGGCCT 1 cut(s) 788
StyD4I CCNGG 6 cut(s) 42, 318, 1220, 1354, 1355, 1663
StyI CCWWGG 1 cut(s) 651
TaiI ACGT 9 cut(s) 15, 82, 225, 272, 1140, 1215, 1481, 1934, 1964
TasI AATT 6 cut(s) 339, 636, 656, 796, 1033, 1075
TatI WGTACW 2 cut(s) 851, 1058
TfiI GAWTC 5 cut(s) 947, 958, 1087, 1593, 2076
Tru1I TTAA 6 cut(s) 684, 879, 927, 954, 1194, 1404
Tru9I TTAA 6 cut(s) 684, 879, 927, 954, 1194, 1404
TscAI CASTG 6 cut(s) 1479, 1557, 1787, 1962, 2040, 2270
TseFI GTSAC 7 cut(s) 146, 344, 1474, 1628, 1853, 1957, 2111
TseI GCWGC 2 cut(s) 23, 47
Tsp45I GTSAC 7 cut(s) 146, 344, 1474, 1628, 1853, 1957, 2111
TspDTI ATGAA 6 cut(s) 572, 1214, 1251, 1336, 1409, 2008
TspGWI ACGGA 2 cut(s) 320, 983
TspMI CCCGGG 1 cut(s) 1355
TspRI CASTG 6 cut(s) 1479, 1557, 1787, 1962, 2040, 2270
Van91I CCANNNNNTGG 1 cut(s) 1750
Vha464I CTTAAG 2 cut(s) 953, 1403
VpaK11BI GGWCC 2 cut(s) 386, 2040
XapI RAATTY 3 cut(s) 339, 656, 1075
XceI RCATGY 1 cut(s) 1703
XmaI CCCGGG 1 cut(s) 1355
XmiI GTMKAC 2 cut(s) 1215, 2004
XmnI GAANNNNTTC 2 cut(s) 830, 1976
XspI CTAG 4 cut(s) 536, 861, 1208, 1692
ZraI GACGTC 1 cut(s) 1932
ZrmI AGTACT 1 cut(s) 853
Zsp2I ATGCAT 1 cut(s) 1729
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.