Rorug02G0166700

Cyclic nucleotide-gated ion channel 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000119
Physical Location & Seq
Forward (+)
14925724 .. 14929363
3640 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0166700.1

Sequence Viewer

Length: 2649 bp
ATGGTTGGTGGAGGTGATGTTTTTGGTGGGGCTGCTGGAGGATTGCTGTTCAATGTTCTCTATGATGCGCTTAAAGAGCTCATCAGCAAGTCTATTATGTATAAACCTCTCCTCCAAAACATTCTATCTGCGCTAGACTCATATGGACCAGTGATCAAACAGATAGAAGCAAGCAATGAGGAACTGGGTCTTCCAAAGGAGGAAGTAGAAAAGTTCAAAGCACATATGGAGGAGGGCATAGAGATAGTCCGGAAGTGCTCTAAGGTTCGCAAGTGGAACATCTACAAGAAGTACAAGTACGCCAACAAACTTCTTGGATGGGATGGTGCTCTTGGAAGACAGTTGACAGTACTGCAGGCGCAGGGAGTAAGGGATGGGAAGAAGACCTTGGTTTCGGTAAAGCAGATAGAGAGGGTGGTCACTGGGATTGAAACGAGTAGTATGTTTTTAGAGGCAGGGGTTGCGCATATTGCAAAAACCAGTAGGCATATGGAGAAGGCGCTCAGCGGAGTTGAAATGATAAGTGAAGATATAGTGGAGGGGATGTACCGAATTGAAGACCGAGTTGAAACAAGCAGTAGCATTGTGGAGGCAGGGTTGAAGCGGATTGAAGACCGACTTGAGACACGAAATGGAGCTAAAGTTGGAGCTTGGGGTGTTGTGCCTGAACTTGCACAGTTTACGGTTGGATTGGATGAGCCTTTGAAGGAATTGAAGATGAAACTTCTCAAGGAGGATGGAGTCTCGATGCTAGTGGTCACTGCTCCCGGAGGATGTGGGAAAACTACATTGGCAACAAAGTTTTGTCAAGATGAGCAAGTCAAAGATAAATTCAAGAATAATATCTTCTTTGTCACTGTGTCAAACACACCAAACCTGAGCCTTATTGTGCAAGAGCTATACCAACGCAAGGGTTCCCAGGTGCCTACTTTCCAAAATGAAATAACCGCAGTCAAGTGGCTGCAAACATTTCTGAAGGAAGAAGGACAAAACCCTTTACTATTAGTCTTGGATGATGTTTGGTCCGGATCAGAATCCCTACTAGACAAGTTTCAGTTTGAGATGCCAGATTACAAGATTTTGGTCACATCAAGATCTGAATTTCCGAGATTTGGTTCCTCATATCATTTGCGATCATTGGATTATGACAATGCCATGAAACTTTTTCATCATTCAGCATCTTTGGGAGATAAAAGCTCACATATTCCAGAATACCTCTCGAGTCGGATAGTAAAGGGCTGTAAGGGATTTCCACTTGCAATTACGGTGGCAGGAGGATCACTTTGCGGACAAAGAATAGAGATCTGGCAACAAATACTAATTGAATGGTCCAAAGGGTCTTCTATTCTTGATTCTGAGACCACATTGCTTCTTCGCCTCCAAAGCAGCTTAGTTGTCTCGGACTCGGATAAAGAAATGGCTATCATCAGAAAGTGTTTCTTAGACCTTGCTTCATTTCCTGAAGACCAAAGAATCCCAGTTACTGCCCTCATTGATATGTGGACAGAGTCATATGATCAGCTAGATGAAGATATTTTGTGTGTTGCATACCTCCAGAGGCTGACTAACCGAAGTCTGGCTAATCTTGTAGTCACACGGAAGGACCAGAGCCTGGGGGATGGATACTACAGCGAACATTTTGTTACCCAGCATGATCTGCTTAGAGAGTTGGCTATCTATGAGACAAAACAACTAGACCCAGGACATAGAGAAAGACTGATTATAAACATATCTGGAGACAATTTTCCCAAGTGGTGGAGTGAAAAGAAGCATGAATCCTTGAAAGCTCGCTTATTATCCATCTCAACTGATGGAAATTTCTCATCAAAATGGCACCACATGGAACTACCATATGCTGAGGTTCTAGTCTTGAATTTTCAGACAAAGAACTACGCCTTACCCAAATTTGTGGACACTATGCGTAAATTGAAGGTACTTGTTGTCACAAATTATGGTTTCTCGCCTGCTGAACTAAGTAATATCCCACTACTGGGTTCGTTATCTCATCTAAAGAGAATTAGATTGGAGCGTGTCTCAATACCATCAATGTCCAAGAACCCCATACAATTGGTGGGTCTAAAAAAGATATCTCTATTCATGTGTACCATTGGGAAAGCTTTCAGTGACGGTTCCATCCAGATTTCGTACGCATTTCCAAGTCTAGAGGAAATTAGCATTGACTATTGCAATGACTTGGTGGAAATGCCTGCCGACGTCTGTGATCTTAGTCATCTACGGAATCTCAGTATCACCAACTCACATAAGCTTCTTGCCTTGCCTGAAGAAATTGGAAAACTGGAAAAGTTGGAACTGCTGAGACTAAGGTCATGTACAGACTTGGCACAGTTGCCAGGCTCGAGCAGGAACCTAAAGAAGTTGATCTTTCTTGACATTTCTTATTGCTTTAGCATCAAGGTGTTGCCTGGAGATATTGGTGAAATGAGCAGCTTAAGAAAGCTCAACATGAGAGAATGCTCAAGATTGCAAGAAGTGCCTGTGTCGGTTTTGGATCTTGAGCATTTGGAGGAAGTGATATGTGATGATGAGACAAGAAATTTATGGGAACCATTCTTACCTCTTGGAAACACAAGAATAGTGGTGGAAAAAGAAACTGTCAACCTAAATTGGCTCCATAAGCTTCAATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
Ion_trans

Protein Analysis

882

Amino Acids

99.5

Weight (kDa)

6.21

Isoelectric Point (pI)

46.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 6 - 137 3.6e-24 Arabidopsis broad-spectrum mildew resistance protein RPW8
NB-ARC PF00931 233 - 392 1.5e-16 NB-ARC domain
LRR_14 PF23598 724 - 843 5.5e-14 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000119)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g10570 FvH4_4g36390 FvH4_7g31840 FvH4_7g31840 FvH4_7g31850 FvH4_7g31850 FvH4_7g31861 FvH4_7g31861 FvH4_7g31950
malus_domestica MD01G1054600.v1.1 MD01G1221100.v1.1 MD01G1221400.v1.1 MD01G1221600.v1.1 MD01G1221800.v1.1 MD01G1222000.v1.1 MD01G1222100.v1.1 MD07G1140800.v1.1 MD07G1291200.v1.1 MD07G1291400.v1.1 MD07G1291500.v1.1 MD07G1291700.v1.1 MD07G1292500.v1.1 MD07G1292700.v1.1 MD07G1292800.v1.1 MD07G1292900.v1.1 MD08G1241600.v1.1 MD11G1083800.v1.1 MD15G1431600.v1.1
prunus_persica Prupe.1G017800_v2.0.a1 Prupe.1G017800_v2.0.a1 Prupe.1G162800_v2.0.a1 Prupe.2G312500_v2.0.a1 Prupe.2G312500_v2.0.a1 Prupe.2G312700_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092800_v2.0.a1 Prupe.4G092800_v2.0.a1 Prupe.6G142800_v2.0.a1 Prupe.6G142800_v2.0.a1 Prupe.6G143000_v2.0.a1 Prupe.6G143100_v2.0.a1 Prupe.6G143100_v2.0.a1 Prupe.6G143100_v2.0.a1 Prupe.6G143100_v2.0.a1
pyrus_communis pycom01g23050 pycom01g23070 pycom01g23090 pycom01g23100 pycom01g23110 pycom01g23120 pycom01g23170 pycom07g26410 pycom07g26440 pycom07g26470 pycom07g26480 pycom07g26490 pycom07g26500 pycom07g26510 pycom07g26530 pycom07g26540 pycom07g26570 pycom07g26580 pycom07g26590 pycom08g20940
rosa_chinensis RchiOBHm_Chr1g0324571 RchiOBHm_Chr1g0336181 RchiOBHm_Chr1g0381061 RchiOBHm_Chr1g0381091 RchiOBHm_Chr1g0381121 RchiOBHm_Chr1g0381131 RchiOBHm_Chr1g0381141 RchiOBHm_Chr1g0381161 RchiOBHm_Chr1g0381211 RchiOBHm_Chr2g0110361 RchiOBHm_Chr2g0120231 RchiOBHm_Chr3g0486281 RchiOBHm_Chr3g0486311 RchiOBHm_Chr3g0486401 RchiOBHm_Chr4g0409511 RchiOBHm_Chr4g0409521 RchiOBHm_Chr5g0017271 RchiOBHm_Chr5g0048781 RchiOBHm_Chr5g0048811
rosa_laevigata RLG00000002401 RLG00000008559 RLG00000017798 RLG00000017799 RLG00000023082 RLG00000023088 RLG00000026230 RLG00000026238 RLG00000026240 RLG00000026241 RLG00000026242 RLG00000026243 RLG00000026244 RLG00000026246 RLG00000026247 RLG00000026248 RLG00000029876 RLG00000030189 RLG00000034579
rosa_multiflora Rmu_co8101374.1_g000001 Rmu_co8309149.1_g000001 Rmu_co8450063.1_g000001 Rmu_sc0000441.1_g000094 Rmu_sc0000479.1_g000005 Rmu_sc0000647.1_g000006 Rmu_sc0001393.1_g000031 Rmu_sc0001423.1_g000037 Rmu_sc0001616.1_g000015 Rmu_sc0001857.1_g000011 Rmu_sc0002183.1_g000013 Rmu_sc0002183.1_g000028 Rmu_sc0002183.1_g000030 Rmu_sc0002688.1_g000003 Rmu_sc0002688.1_g000020 Rmu_sc0003801.1_g000003 Rmu_sc0003801.1_g000004 Rmu_sc0004125.1_g000006 Rmu_sc0004494.1_g000005 Rmu_sc0004494.1_g000006 Rmu_sc0004494.1_g000008 Rmu_sc0004494.1_g000009 Rmu_sc0005842.1_g000004 Rmu_sc0006872.1_g000001 Rmu_sc0006872.1_g000005 Rmu_sc0006872.1_g000009 Rmu_sc0006872.1_g000010 Rmu_sc0008053.1_g000026
rosa_roxburghii Rroxscaffold_159G00432960 Rroxscaffold_1G00033060 Rroxscaffold_2G00123150 Rroxscaffold_2G00123170 Rroxscaffold_2G00133500 Rroxscaffold_2G00133550 Rroxscaffold_4G00278650 Rroxscaffold_4G00278750 Rroxscaffold_4G00278790 Rroxscaffold_4G00278800 Rroxscaffold_4G00278810 Rroxscaffold_4G00325390 Rroxscaffold_5G00354010 Rroxscaffold_6G00395890 Rroxscaffold_6G00395900 Rroxscaffold_6G00395920
rosa_rugosa Rorug01G0046900.1 Rorug01G0427000 Rorug01G0427100 Rorug01G0427200 Rorug01G0427300 Rorug01G0427400 Rorug01G0427500 Rorug01G0427500 Rorug01G0427600 Rorug01G0427600 Rorug01G0427700 Rorug01G0427700 Rorug01G0427800 Rorug01G0428700 Rorug02G0166400 Rorug02G0166500 Rorug02G0166500 Rorug02G0166600 Rorug02G0166600 Rorug02G0166700 Rorug02G0167300 Rorug02G0167300 Rorug02G0167300 Rorug03G0224600 Rorug03G0224600 Rorug04G0094400 Rorug05G0241100 Rorug05G0241200
rosa_samantha Rh1BG407700 Rh1BG407900 Rh1BG409000 Rh1CG064700 Rh1CG420900 Rh1CG421000 Rh1CG421200 Rh1CG421300 Rh1CG421800 Rh1CG421900 Rh1CG422000 Rh1CG422200 Rh1CG422300 Rh1CG423600 Rh1DG437600 Rh1DG438900 Rh1DG439000 Rh1DG440300 Rh2AG219100 Rh2CG220700 Rh2DG225300 Rh3AG274400 Rh3AG274600 Rh3CG308900 Rh3DG305800 Rh4AG156400 Rh5AG129000 Rh5AG320900 Rh5CG139600
rosa_wichuraiana Rw1G005300 Rw1G039280 Rw1G039300 Rw1G039310 Rw1G039320 Rw1G039330 Rw1G039340 Rw1G039360 Rw1G039390 Rw2G016910 Rw2G022800 Rw3G024330 Rw3G024340 Rw3G024350 Rw3G024400 Rw4G012810 Rw5G030120 Rw5G030280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1724
AatII GACGTC 1 cut(s) 2217
Acc16I TGCGCA 1 cut(s) 465
AccB1I GGYRCC 2 cut(s) 922, 1833
AccB7I CCANNNNNTGG 2 cut(s) 1612, 1755
AccIII TCCGGA 2 cut(s) 249, 1025
AciI CCGC 4 cut(s) 507, 604, 948, 1287
AclWI GGATC 3 cut(s) 1036, 1285, 2517
AcsI RAATTY 6 cut(s) 830, 1100, 1816, 1873, 1904, 2554
AcuI CTGAAG 3 cut(s) 995, 1482, 2301
AcyI GRCGYC 1 cut(s) 2214
AfaI GTAC 8 cut(s) 293, 299, 351, 548, 1935, 2104, 2147, 2332
AfiI CCNNNNNNNGG 7 cut(s) 910, 1112, 1576, 1612, 1755, 1990, 1991
AflII CTTAAG 1 cut(s) 2449
AjnI CCWGG 5 cut(s) 918, 1611, 1699, 2350, 2422
AjuI GAANNNNNNNTTGG 4 cut(s) 371, 403, 773, 805
Alw21I GWGCWC 3 cut(s) 81, 260, 331
Alw26I GTCTC 9 cut(s) 617, 748, 1352, 1402, 1676, 1731, 2038, 2311, 2540
AlwI GGATC 3 cut(s) 1036, 1285, 2517
AlwNI CAGNNNCTG 3 cut(s) 1484, 1561, 1612
Ama87I CYCGRG 2 cut(s) 1219, 2356
Aor13HI TCCGGA 2 cut(s) 249, 1025
ApeKI GCWGC 4 cut(s) 32, 961, 1386, 2445
ApoI RAATTY 6 cut(s) 830, 1100, 1816, 1873, 1904, 2554
ArsI GACNNNNNNTTYG 2 cut(s) 376, 408
Asp700I GAANNNNTTC 1 cut(s) 2117
AspLEI GCGC 5 cut(s) 70, 133, 361, 466, 502
AspS9I GGNCC 4 cut(s) 146, 1023, 1329, 1603
AsuC2I CCSGG 1 cut(s) 768
AsuHPI GGTGA 3 cut(s) 26, 2242, 2447
AvaI CYCGRG 2 cut(s) 1219, 2356
AvaII GGWCC 4 cut(s) 146, 1023, 1329, 1603
BanI GGYRCC 2 cut(s) 922, 1833
BanII GRGCYC 1 cut(s) 81
BarI GAAGNNNNNNTAC 2 cut(s) 281, 313
BbsI GAAGAC 7 cut(s) 182, 343, 389, 564, 618, 1332, 1470
Bbv12I GWGCWC 3 cut(s) 81, 260, 331
BbvCI CCTCAGC 1 cut(s) 1857
BbvI GCAGC 4 cut(s) 19, 948, 1398, 2457
BccI CCATC 9 cut(s) 312, 317, 368, 731, 1613, 1805, 1808, 2050, 2141
BciT130I CCWGG 5 cut(s) 920, 1613, 1701, 2352, 2424
BciVI GTATCC 1 cut(s) 1616
BclI TGATCA 2 cut(s) 153, 1516
BcnI CCSGG 1 cut(s) 768
BcoDI GTCTC 9 cut(s) 617, 748, 1352, 1402, 1676, 1731, 2038, 2311, 2540
BfaI CTAG 7 cut(s) 134, 752, 1043, 1523, 1694, 1865, 2162
BfmI CTRYAG 2 cut(s) 353, 1627
BfoI RGCGCY 1 cut(s) 503
BfrI CTTAAG 1 cut(s) 2449
BfuI GTATCC 1 cut(s) 1616
BglII AGATCT 2 cut(s) 1094, 1302
BisI GCNGC 4 cut(s) 33, 962, 1387, 2446
BlpI GCTNAGC 1 cut(s) 503
BlsI GCNGC 4 cut(s) 34, 963, 1388, 2447
BmcAI AGTACT 1 cut(s) 351
Bme1390I CCNGG 6 cut(s) 768, 920, 1613, 1701, 2352, 2424
Bme18I GGWCC 4 cut(s) 146, 1023, 1329, 1603
BmeT110I CYCGRG 2 cut(s) 1219, 2356
BmgT120I GGNCC 4 cut(s) 146, 1023, 1329, 1603
BmiI GGNNCC 8 cut(s) 916, 924, 1117, 1835, 2131, 2366, 2565, 2630
BmrFI CCNGG 6 cut(s) 768, 920, 1613, 1701, 2352, 2424
BmrI ACTGGG 4 cut(s) 194, 432, 1472, 2000
BmsI GCATC 5 cut(s) 55, 738, 1053, 1187, 2418
BmuI ACTGGG 4 cut(s) 194, 432, 1472, 2000
BoxI GACNNNNGTC 1 cut(s) 2323
BpiI GAAGAC 7 cut(s) 182, 343, 389, 564, 618, 1332, 1470
BplI GAGNNNNNCTC 2 cut(s) 2018, 2050
BpmI CTGGAG 4 cut(s) 57, 1538, 1755, 2445
Bpu10I CCTNAGC 2 cut(s) 878, 1857
Bpu1102I GCTNAGC 1 cut(s) 503
BpuEI CTTGAG 4 cut(s) 641, 713, 2461, 2534
BpuMI CCSGG 1 cut(s) 768
BsaBI GATNNNNATC 1 cut(s) 1033
BsaHI GRCGYC 1 cut(s) 2214
BsaI GGTCTC 1 cut(s) 1352
BsaJI CCNNGG 4 cut(s) 387, 918, 1612, 1699
BsaWI WCCGGW 2 cut(s) 249, 1025
BsaXI ACNNNNNCTCC 9 cut(s) 33, 96, 126, 530, 560, 581, 611, 627, 657
Bsc4I CCNNNNNNNGG 7 cut(s) 910, 1112, 1576, 1612, 1755, 1990, 1991
Bse1I ACTGG 7 cut(s) 149, 189, 427, 480, 1478, 1995, 2301
Bse3DI GCAATG 3 cut(s) 181, 1364, 2194
Bse8I GATNNNNATC 1 cut(s) 1033
BseAI TCCGGA 2 cut(s) 249, 1025
BseBI CCWGG 5 cut(s) 920, 1613, 1701, 2352, 2424
BseDI CCNNGG 4 cut(s) 387, 918, 1612, 1699
BseJI GATNNNNATC 1 cut(s) 1033
BseLI CCNNNNNNNGG 7 cut(s) 910, 1112, 1576, 1612, 1755, 1990, 1991
BseMI GCAATG 3 cut(s) 181, 1364, 2194
BseMII CTCAG 6 cut(s) 517, 869, 1347, 1848, 2257, 2306
BseNI ACTGG 7 cut(s) 149, 189, 427, 480, 1478, 1995, 2301
BseRI GAGGAG 2 cut(s) 101, 245
BseXI GCAGC 4 cut(s) 19, 948, 1398, 2457
BseYI CCCAGC 1 cut(s) 1647
BshNI GGYRCC 2 cut(s) 922, 1833
BsiHKAI GWGCWC 3 cut(s) 81, 260, 331
BsiHKCI CYCGRG 2 cut(s) 1219, 2356
BsiSI CCGG 3 cut(s) 250, 768, 1026
BsiWI CGTACG 1 cut(s) 2145
BslI CCNNNNNNNGG 7 cut(s) 910, 1112, 1576, 1612, 1755, 1990, 1991
BsmAI GTCTC 9 cut(s) 617, 748, 1352, 1402, 1676, 1731, 2038, 2311, 2540
BsmI GAATGC 1 cut(s) 2477
Bso31I GGTCTC 1 cut(s) 1352
BsoBI CYCGRG 2 cut(s) 1219, 2356
Bsp1286I GDGCHC 3 cut(s) 81, 260, 331
Bsp13I TCCGGA 2 cut(s) 249, 1025
Bsp1407I TGTACA 1 cut(s) 2330
Bsp1720I GCTNAGC 1 cut(s) 503
BspACI CCGC 4 cut(s) 507, 604, 948, 1287
BspCNI CTCAG 6 cut(s) 516, 870, 1348, 1849, 2256, 2307
BspEI TCCGGA 2 cut(s) 249, 1025
BspLI GGNNCC 8 cut(s) 916, 924, 1117, 1835, 2131, 2366, 2565, 2630
BspMAI CTGCAG 1 cut(s) 357
BspPI GGATC 3 cut(s) 1036, 1285, 2517
BspT107I GGYRCC 2 cut(s) 922, 1833
BspTI CTTAAG 1 cut(s) 2449
BspTNI GGTCTC 1 cut(s) 1352
BsrDI GCAATG 3 cut(s) 181, 1364, 2194
BsrGI TGTACA 1 cut(s) 2330
BsrI ACTGG 7 cut(s) 149, 189, 427, 480, 1478, 1995, 2301
BssECI CCNNGG 4 cut(s) 387, 918, 1612, 1699
BssNI GRCGYC 1 cut(s) 2214
BssT1I CCWWGG 1 cut(s) 387
Bst2UI CCWGG 5 cut(s) 920, 1613, 1701, 2352, 2424
Bst4CI ACNGT 9 cut(s) 342, 349, 678, 685, 859, 1267, 2129, 2346, 2614
BstACI GRCGYC 1 cut(s) 2214
BstAFI CTTAAG 1 cut(s) 2449
BstAPI GCANNNNNTGC 3 cut(s) 461, 1657, 2491
BstAUI TGTACA 1 cut(s) 2330
BstC8I GCNNGC 5 cut(s) 172, 357, 1789, 1965, 2208
BstH2I RGCGCY 1 cut(s) 503
BstHHI GCGC 5 cut(s) 70, 133, 361, 466, 502
BstMAI GTCTC 9 cut(s) 617, 748, 1352, 1402, 1676, 1731, 2038, 2311, 2540
BstMWI GCNNNNNNNGC 7 cut(s) 76, 461, 470, 1383, 1657, 2491, 2635
BstNI CCWGG 5 cut(s) 920, 1613, 1701, 2352, 2424
BstPAI GACNNNNGTC 1 cut(s) 2323
BstSCI CCNGG 6 cut(s) 766, 918, 1611, 1699, 2350, 2422
BstSFI CTRYAG 2 cut(s) 353, 1627
BstV1I GCAGC 4 cut(s) 19, 948, 1398, 2457
BstV2I GAAGAC 7 cut(s) 182, 343, 389, 564, 618, 1332, 1470
BstX2I RGATCY 3 cut(s) 1094, 1302, 2509
BstXI CCANNNNNNTGG 2 cut(s) 1909, 2068
BstYI RGATCY 3 cut(s) 1094, 1302, 2509
BsuI GTATCC 1 cut(s) 1616
BtsI GCAGTG 1 cut(s) 759
BtsIMutI CAGTG 5 cut(s) 156, 420, 759, 855, 2128
Cac8I GCNNGC 5 cut(s) 172, 357, 1789, 1965, 2208
CaiI CAGNNNCTG 3 cut(s) 1484, 1561, 1612
CfoI GCGC 5 cut(s) 70, 133, 361, 466, 502
Cfr13I GGNCC 4 cut(s) 146, 1023, 1329, 1603
Csp6I GTAC 8 cut(s) 292, 298, 350, 547, 1934, 2103, 2146, 2331
CspCI CAANNNNNGTGG 4 cut(s) 1248, 1283, 2577, 2612
CviAII CATG 7 cut(s) 1156, 1652, 1772, 1840, 2098, 2328, 2464
CviQI GTAC 8 cut(s) 292, 298, 350, 547, 1934, 2103, 2146, 2331
Ecl136II GAGCTC 1 cut(s) 79
Eco130I CCWWGG 1 cut(s) 387
Eco24I GRGCYC 1 cut(s) 81
Eco31I GGTCTC 1 cut(s) 1352
Eco32I GATATC 1 cut(s) 2088
Eco47I GGWCC 4 cut(s) 146, 1023, 1329, 1603
Eco53kI GAGCTC 1 cut(s) 79
Eco57I CTGAAG 3 cut(s) 995, 1482, 2301
Eco88I CYCGRG 2 cut(s) 1219, 2356
EcoICRI GAGCTC 1 cut(s) 79
EcoRII CCWGG 5 cut(s) 918, 1611, 1699, 2350, 2422
EcoRV GATATC 1 cut(s) 2088
EcoT14I CCWWGG 1 cut(s) 387
EcoT38I GRGCYC 1 cut(s) 81
ErhI CCWWGG 1 cut(s) 387
FaeI CATG 7 cut(s) 1159, 1655, 1775, 1843, 2101, 2331, 2467
FalI AAGNNNNNCTT 8 cut(s) 371, 403, 603, 635, 1424, 1456, 2366, 2398
FatI CATG 7 cut(s) 1155, 1651, 1771, 1839, 2097, 2327, 2463
FauNDI CATATG 5 cut(s) 142, 225, 489, 1513, 1852
FbaI TGATCA 2 cut(s) 153, 1516
Fnu4HI GCNGC 4 cut(s) 33, 962, 1387, 2446
FriOI GRGCYC 1 cut(s) 81
Fsp4HI GCNGC 4 cut(s) 33, 962, 1387, 2446
FspBI CTAG 7 cut(s) 134, 752, 1043, 1523, 1694, 1865, 2162
FspI TGCGCA 1 cut(s) 465
GlaI GCGC 5 cut(s) 69, 132, 360, 465, 501
GluI GCNGC 4 cut(s) 33, 962, 1387, 2446
GsaI CCCAGC 1 cut(s) 1651
GsuI CTGGAG 4 cut(s) 57, 1538, 1755, 2445
HaeII RGCGCY 1 cut(s) 503
HapII CCGG 3 cut(s) 250, 768, 1026
HhaI GCGC 5 cut(s) 70, 133, 361, 466, 502
Hin1I GRCGYC 1 cut(s) 2214
Hin1II CATG 7 cut(s) 1159, 1655, 1775, 1843, 2101, 2331, 2467
Hin6I GCGC 5 cut(s) 68, 131, 359, 464, 500
HinP1I GCGC 5 cut(s) 68, 131, 359, 464, 500
HincII GTYRAC 2 cut(s) 345, 2617
HindII GTYRAC 2 cut(s) 345, 2617
HindIII AAGCTT 3 cut(s) 2115, 2264, 2636
HpaII CCGG 3 cut(s) 250, 768, 1026
HphI GGTGA 3 cut(s) 26, 2242, 2447
Hpy166II GTNNAC 6 cut(s) 345, 681, 1503, 1912, 2103, 2617
Hpy8I GTNNAC 6 cut(s) 345, 681, 1503, 1912, 2103, 2617
Hpy99I CGWCG 1 cut(s) 2216
HpyAV CCTTC 6 cut(s) 490, 700, 970, 977, 1594, 1924
HpyCH4III ACNGT 9 cut(s) 342, 349, 678, 685, 859, 1267, 2129, 2346, 2614
HpyCH4IV ACGT 1 cut(s) 2214
HpyCH4V TGCA 9 cut(s) 355, 473, 674, 892, 964, 1259, 1547, 2187, 2485
HpyF10VI GCNNNNNNNGC 7 cut(s) 76, 461, 470, 1383, 1657, 2491, 2635
HpySE526I ACGT 1 cut(s) 2214
Hsp92I GRCGYC 1 cut(s) 2214
Hsp92II CATG 7 cut(s) 1159, 1655, 1775, 1843, 2101, 2331, 2467
HspAI GCGC 5 cut(s) 68, 131, 359, 464, 500
Kpn2I TCCGGA 2 cut(s) 249, 1025
Ksp22I TGATCA 2 cut(s) 153, 1516
LmnI GCTCC 5 cut(s) 635, 647, 769, 2026, 2634
Lsp1109I GCAGC 4 cut(s) 19, 948, 1398, 2457
LweI GCATC 5 cut(s) 55, 738, 1053, 1187, 2418
MaeI CTAG 7 cut(s) 134, 752, 1043, 1523, 1694, 1865, 2162
MaeII ACGT 1 cut(s) 2214
MaeIII GTNAC 9 cut(s) 418, 757, 853, 1084, 1480, 1591, 1642, 1942, 2123
MfeI CAATTG 1 cut(s) 2066
MflI RGATCY 3 cut(s) 1094, 1302, 2509
MhlI GDGCHC 3 cut(s) 81, 260, 331
MlyI GAGTC 5 cut(s) 131, 750, 1231, 1397, 1517
MmeI TCCRAC 4 cut(s) 625, 667, 1205, 2286
MroI TCCGGA 2 cut(s) 249, 1025
MroXI GAANNNNTTC 1 cut(s) 2117
MseI TTAA 2 cut(s) 72, 2450
MslI CAYNNNNRTG 3 cut(s) 1496, 1828, 2414
MspA1I CMGCKG 1 cut(s) 507
MspCI CTTAAG 1 cut(s) 2449
MspI CCGG 3 cut(s) 250, 768, 1026
MspR9I CCNGG 6 cut(s) 768, 920, 1613, 1701, 2352, 2424
MunI CAATTG 1 cut(s) 2066
Mva1269I GAATGC 1 cut(s) 2477
MvaI CCWGG 5 cut(s) 920, 1613, 1701, 2352, 2424
MwoI GCNNNNNNNGC 7 cut(s) 76, 461, 470, 1383, 1657, 2491, 2635
NciI CCSGG 1 cut(s) 768
NdeI CATATG 5 cut(s) 142, 225, 489, 1513, 1852
NlaIII CATG 7 cut(s) 1159, 1655, 1775, 1843, 2101, 2331, 2467
NlaIV GGNNCC 8 cut(s) 916, 924, 1117, 1835, 2131, 2366, 2565, 2630
NmuCI GTSAC 7 cut(s) 418, 757, 853, 1084, 1591, 1942, 2123
NsbI TGCGCA 1 cut(s) 465
PaeR7I CTCGAG 2 cut(s) 1219, 2356
PctI GAATGC 1 cut(s) 2477
PdmI GAANNNNTTC 1 cut(s) 2117
PfeI GAWTC 5 cut(s) 1034, 1352, 1473, 1775, 2239
Pfl23II CGTACG 1 cut(s) 2145
PflFI GACNNNGTC 1 cut(s) 1507
PflMI CCANNNNNTGG 2 cut(s) 1612, 1755
PfoI TCCNGGA 1 cut(s) 766
PkrI GCNGC 4 cut(s) 34, 963, 1388, 2447
PleI GAGTC 5 cut(s) 131, 749, 1230, 1397, 1516
PpsI GAGTC 5 cut(s) 131, 749, 1230, 1397, 1516
PshAI GACNNNNGTC 1 cut(s) 2323
PsiI TTATAA 1 cut(s) 1724
Psp124BI GAGCTC 1 cut(s) 81
Psp6I CCWGG 5 cut(s) 918, 1611, 1699, 2350, 2422
PspFI CCCAGC 1 cut(s) 1647
PspGI CCWGG 5 cut(s) 918, 1611, 1699, 2350, 2422
PspLI CGTACG 1 cut(s) 2145
PspN4I GGNNCC 8 cut(s) 916, 924, 1117, 1835, 2131, 2366, 2565, 2630
PspPI GGNCC 4 cut(s) 146, 1023, 1329, 1603
PspXI VCTCGAGB 1 cut(s) 2356
PsrI GAACNNNNNNTAC 2 cut(s) 2556, 2588
PstI CTGCAG 1 cut(s) 357
PstNI CAGNNNCTG 3 cut(s) 1484, 1561, 1612
PsuI RGATCY 3 cut(s) 1094, 1302, 2509
PsyI GACNNNGTC 1 cut(s) 1507
RsaI GTAC 8 cut(s) 293, 299, 351, 548, 1935, 2104, 2147, 2332
RsaNI GTAC 8 cut(s) 292, 298, 350, 547, 1934, 2103, 2146, 2331
RseI CAYNNNNRTG 3 cut(s) 1496, 1828, 2414
SacI GAGCTC 1 cut(s) 81
SaqAI TTAA 2 cut(s) 72, 2450
SatI GCNGC 4 cut(s) 33, 962, 1387, 2446
Sau96I GGNCC 4 cut(s) 146, 1023, 1329, 1603
ScaI AGTACT 1 cut(s) 351
SchI GAGTC 5 cut(s) 131, 750, 1231, 1397, 1517
ScrFI CCNGG 6 cut(s) 768, 920, 1613, 1701, 2352, 2424
SduI GDGCHC 3 cut(s) 81, 260, 331
SfaNI GCATC 5 cut(s) 55, 738, 1053, 1187, 2418
SfcI CTRYAG 2 cut(s) 353, 1627
Sfr274I CTCGAG 2 cut(s) 1219, 2356
SinI GGWCC 4 cut(s) 146, 1023, 1329, 1603
SlaI CTCGAG 2 cut(s) 1219, 2356
SmiMI CAYNNNNRTG 3 cut(s) 1496, 1828, 2414
SmlI CTYRAG 7 cut(s) 620, 728, 1219, 2356, 2449, 2476, 2513
SmoI CTYRAG 7 cut(s) 620, 728, 1219, 2356, 2449, 2476, 2513
SsiI CCGC 4 cut(s) 507, 604, 948, 1287
SspMI CTAG 7 cut(s) 134, 752, 1043, 1523, 1694, 1865, 2162
SstI GAGCTC 1 cut(s) 81
StyD4I CCNGG 6 cut(s) 766, 918, 1611, 1699, 2350, 2422
StyI CCWWGG 1 cut(s) 387
TaaI ACNGT 9 cut(s) 342, 349, 678, 685, 859, 1267, 2129, 2346, 2614
TaiI ACGT 1 cut(s) 2217
TaqI TCGA 3 cut(s) 746, 1220, 2357
TaqII GACCGA 2 cut(s) 576, 630
TatI WGTACW 3 cut(s) 291, 349, 2330
TfiI GAWTC 5 cut(s) 1034, 1352, 1473, 1775, 2239
Tru1I TTAA 2 cut(s) 72, 2450
Tru9I TTAA 2 cut(s) 72, 2450
TscAI CASTG 5 cut(s) 156, 427, 766, 862, 2128
TseFI GTSAC 7 cut(s) 418, 757, 853, 1084, 1591, 1942, 2123
TseI GCWGC 4 cut(s) 32, 961, 1386, 2445
Tsp45I GTSAC 7 cut(s) 418, 757, 853, 1084, 1591, 1942, 2123
TspDTI ATGAA 8 cut(s) 734, 954, 1157, 1172, 1443, 1542, 1788, 2086
TspGWI ACGGA 2 cut(s) 1612, 2251
TspRI CASTG 5 cut(s) 156, 427, 766, 862, 2128
Tth111I GACNNNGTC 1 cut(s) 1507
Van91I CCANNNNNTGG 2 cut(s) 1612, 1755
Vha464I CTTAAG 1 cut(s) 2449
VpaK11BI GGWCC 4 cut(s) 146, 1023, 1329, 1603
XapI RAATTY 6 cut(s) 830, 1100, 1816, 1873, 1904, 2554
XbaI TCTAGA 1 cut(s) 2161
XcmI CCANNNNNNNNNTGG 2 cut(s) 487, 2068
XhoI CTCGAG 2 cut(s) 1219, 2356
XmnI GAANNNNTTC 1 cut(s) 2117
XspI CTAG 7 cut(s) 134, 752, 1043, 1523, 1694, 1865, 2162
ZraI GACGTC 1 cut(s) 2215
ZrmI AGTACT 1 cut(s) 351
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.