MD01G1221600.v1.1

Cyclic nucleotide-gated ion channel 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0000119
Physical Location & Seq
Reverse (-)
31378432 .. 31380974
2543 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1221600.v1.1.491

Sequence Viewer

Length: 1695 bp
ATGTTATGGATTTCATTTGTTGTTTGTTGCGTGTTTCCCAGAAAGGCTGGAGAACGTTCGAAAGCAACAAGGAACATAATATTTGCGATATCATGCGTATTTGCTGTCTCTCTGGATCCCTTGTTCTTTTACGTCCTGATCATCGATCAGGATGACAAGTGCCTTCAAATGGACAAAACGTTGAGCATTGTTGCTTGTCTCACGCGATCACTCACGGATGTCATTTTCCTGGGGCATTTTATATGTGAAATTTATGACCGTGTTCAAAATGCAAAACCACAAACCCAGAAAAATGGGGGAAATTCGCATAAGAAAACGAAAATCAGAAAGTTGATTGATCAAGTTGCCATGAAAATAGCTCAGAAGATGCCGTGGTTATCTGTCTTTACTGTAATAGGCTTTTTCGCTCTTCTTCCCCTCCCACAACTGCTAATAGGAGTTATTTTTTACACAATGAAAGGCTCAGGATTAGTGGAACACAAAGACGTTCTGAATTTTTTTCTTCTCTGTCAGTATTTTCCGAGGATTTATCGAATCTACCTATCAGCCGAGGATTTCAAAATGATTAATGGGATGTGGATTAAGGGTCTATACAACATGTTTCTCTACATTCTTTCCAGCCACGTACTCGGAGCTTTTTGGTACTTTTTTTCTATTCAACGAGAGTTAACATGTTGGCAAGAGGCTTGTGTAAATCATAGTAAAGATCCCAGAGCATGTTTGAATACTTTCAATTGCGATAGTCGAAGTTCCACTGCAAGAAATATAACATTTATAAATGAGCATTGCCCGTTAGATACTCCAAATGGTGCTTCATCTTCATTTAACTTTGGAATATTTATTGATTCCCTTCAGAATCAAAACACGGAGCATATACCGTTCAGAAAAAAGTTTTTTTACTCTTTCTGGTGGGGCCTGCGAAATCTAAGGTTGATGGAAGCTACAAAAAAAGAGGAGATGAGACAAAAGATGAAGATGAAGAAGCTAGACGTACACACTTGGATGGCCACAAATGAACTCCCTAATGATTTAAGGGAAGAGATCTTGAGTAGCATAAAGCAAACATTGAAACAAAAAACATTGAAGCAACACATTGACGCTGATCTCCATAATTTTCTCTTCTCCATTCTTCCCGGGAAAACCAGAACATCTTTGAAGCGTTGTCTTTGCATGAAAACACTTCAGAAAGTAAAAATGCTTCAAGGCATGGATGACAAAGTGTTGACGTTGATGTGCGACTATCTGAAGCCAGTGACGTACATTGAGAACAGCAACATTTTTCGAATGGGAGATCCACTCGACTGCGTGCTGATCATTATGGAAGGGACAGTGTGGACCTACGCGTCGAGTGATAGTCAAGCTGGGCGAGGAATCTCATCAATGGGCACCAAGCCCCTCCGGAAAGGTGACTTTTACGGGGAAGAGCTTTTCGATTGGGCACCAGGCAGTTTCACCGAACTTCCAGTCTCCAGCAAACATGTCAAAACTCGGATAAAAGTAGATGCATTTCTGCTCACGGCCAATGACTTGGATAGTGTAGTTTCCAAACACCGGCTACAGTGGAAGAAGGAGGGTACTCTAACTCCAGCGATGAAGATCATGGCAGCTTCTACTATAGCAACAGCATTCCGTAGTGGTCGTCGTCTCCGAGCTATAAACAAAGTATGTTCCTCAACCTCCATCTCTCACCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
Ion_trans

Protein Analysis

565

Amino Acids

64.84

Weight (kDa)

9.39

Isoelectric Point (pI)

44.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ion_trans PF00520 28 - 307 8.5e-06 Ion transport protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000119)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g10570 FvH4_4g36390 FvH4_7g31840 FvH4_7g31840 FvH4_7g31850 FvH4_7g31850 FvH4_7g31861 FvH4_7g31861 FvH4_7g31950
malus_domestica MD01G1054600.v1.1 MD01G1221100.v1.1 MD01G1221400.v1.1 MD01G1221600.v1.1 MD01G1221800.v1.1 MD01G1222000.v1.1 MD01G1222100.v1.1 MD07G1140800.v1.1 MD07G1291200.v1.1 MD07G1291400.v1.1 MD07G1291500.v1.1 MD07G1291700.v1.1 MD07G1292500.v1.1 MD07G1292700.v1.1 MD07G1292800.v1.1 MD07G1292900.v1.1 MD08G1241600.v1.1 MD11G1083800.v1.1 MD15G1431600.v1.1
prunus_persica Prupe.1G017800_v2.0.a1 Prupe.1G017800_v2.0.a1 Prupe.1G162800_v2.0.a1 Prupe.2G312500_v2.0.a1 Prupe.2G312500_v2.0.a1 Prupe.2G312700_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092800_v2.0.a1 Prupe.4G092800_v2.0.a1 Prupe.6G142800_v2.0.a1 Prupe.6G142800_v2.0.a1 Prupe.6G143000_v2.0.a1 Prupe.6G143100_v2.0.a1 Prupe.6G143100_v2.0.a1 Prupe.6G143100_v2.0.a1 Prupe.6G143100_v2.0.a1
pyrus_communis pycom01g23050 pycom01g23070 pycom01g23090 pycom01g23100 pycom01g23110 pycom01g23120 pycom01g23170 pycom07g26410 pycom07g26440 pycom07g26470 pycom07g26480 pycom07g26490 pycom07g26500 pycom07g26510 pycom07g26530 pycom07g26540 pycom07g26570 pycom07g26580 pycom07g26590 pycom08g20940
rosa_chinensis RchiOBHm_Chr1g0324571 RchiOBHm_Chr1g0336181 RchiOBHm_Chr1g0381061 RchiOBHm_Chr1g0381091 RchiOBHm_Chr1g0381121 RchiOBHm_Chr1g0381131 RchiOBHm_Chr1g0381141 RchiOBHm_Chr1g0381161 RchiOBHm_Chr1g0381211 RchiOBHm_Chr2g0110361 RchiOBHm_Chr2g0120231 RchiOBHm_Chr3g0486281 RchiOBHm_Chr3g0486311 RchiOBHm_Chr3g0486401 RchiOBHm_Chr4g0409511 RchiOBHm_Chr4g0409521 RchiOBHm_Chr5g0017271 RchiOBHm_Chr5g0048781 RchiOBHm_Chr5g0048811
rosa_laevigata RLG00000002401 RLG00000008559 RLG00000017798 RLG00000017799 RLG00000023082 RLG00000023088 RLG00000026230 RLG00000026238 RLG00000026240 RLG00000026241 RLG00000026242 RLG00000026243 RLG00000026244 RLG00000026246 RLG00000026247 RLG00000026248 RLG00000029876 RLG00000030189 RLG00000034579
rosa_multiflora Rmu_co8101374.1_g000001 Rmu_co8309149.1_g000001 Rmu_co8450063.1_g000001 Rmu_sc0000441.1_g000094 Rmu_sc0000479.1_g000005 Rmu_sc0000647.1_g000006 Rmu_sc0001393.1_g000031 Rmu_sc0001423.1_g000037 Rmu_sc0001616.1_g000015 Rmu_sc0001857.1_g000011 Rmu_sc0002183.1_g000013 Rmu_sc0002183.1_g000028 Rmu_sc0002183.1_g000030 Rmu_sc0002688.1_g000003 Rmu_sc0002688.1_g000020 Rmu_sc0003801.1_g000003 Rmu_sc0003801.1_g000004 Rmu_sc0004125.1_g000006 Rmu_sc0004494.1_g000005 Rmu_sc0004494.1_g000006 Rmu_sc0004494.1_g000008 Rmu_sc0004494.1_g000009 Rmu_sc0005842.1_g000004 Rmu_sc0006872.1_g000001 Rmu_sc0006872.1_g000005 Rmu_sc0006872.1_g000009 Rmu_sc0006872.1_g000010 Rmu_sc0008053.1_g000026
rosa_roxburghii Rroxscaffold_159G00432960 Rroxscaffold_1G00033060 Rroxscaffold_2G00123150 Rroxscaffold_2G00123170 Rroxscaffold_2G00133500 Rroxscaffold_2G00133550 Rroxscaffold_4G00278650 Rroxscaffold_4G00278750 Rroxscaffold_4G00278790 Rroxscaffold_4G00278800 Rroxscaffold_4G00278810 Rroxscaffold_4G00325390 Rroxscaffold_5G00354010 Rroxscaffold_6G00395890 Rroxscaffold_6G00395900 Rroxscaffold_6G00395920
rosa_rugosa Rorug01G0046900.1 Rorug01G0427000 Rorug01G0427100 Rorug01G0427200 Rorug01G0427300 Rorug01G0427400 Rorug01G0427500 Rorug01G0427500 Rorug01G0427600 Rorug01G0427600 Rorug01G0427700 Rorug01G0427700 Rorug01G0427800 Rorug01G0428700 Rorug02G0166400 Rorug02G0166500 Rorug02G0166500 Rorug02G0166600 Rorug02G0166600 Rorug02G0166700 Rorug02G0167300 Rorug02G0167300 Rorug02G0167300 Rorug03G0224600 Rorug03G0224600 Rorug04G0094400 Rorug05G0241100 Rorug05G0241200
rosa_samantha Rh1BG407700 Rh1BG407900 Rh1BG409000 Rh1CG064700 Rh1CG420900 Rh1CG421000 Rh1CG421200 Rh1CG421300 Rh1CG421800 Rh1CG421900 Rh1CG422000 Rh1CG422200 Rh1CG422300 Rh1CG423600 Rh1DG437600 Rh1DG438900 Rh1DG439000 Rh1DG440300 Rh2AG219100 Rh2CG220700 Rh2DG225300 Rh3AG274400 Rh3AG274600 Rh3CG308900 Rh3DG305800 Rh4AG156400 Rh5AG129000 Rh5AG320900 Rh5CG139600
rosa_wichuraiana Rw1G005300 Rw1G039280 Rw1G039300 Rw1G039310 Rw1G039320 Rw1G039330 Rw1G039340 Rw1G039360 Rw1G039390 Rw2G016910 Rw2G022800 Rw3G024330 Rw3G024340 Rw3G024350 Rw3G024400 Rw4G012810 Rw5G030120 Rw5G030280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 776
AasI GACNNNNNNGTC 1 cut(s) 1342
AccB1I GGYRCC 2 cut(s) 1385, 1438
AccII CGCG 2 cut(s) 205, 1343
AccIII TCCGGA 1 cut(s) 1398
AclI AACGTT 2 cut(s) 55, 179
AclWI GGATC 4 cut(s) 110, 123, 701, 1286
AcoI YGGCCR 2 cut(s) 1005, 1518
AcsI RAATTY 3 cut(s) 249, 301, 493
AcuI CTGAAG 3 cut(s) 836, 1166, 1265
AfaI GTAC 5 cut(s) 627, 644, 993, 1259, 1576
AfiI CCNNNNNNNGG 2 cut(s) 169, 1551
AflIII ACRYGT 4 cut(s) 597, 671, 1341, 1477
AjnI CCWGG 2 cut(s) 228, 1441
AjuI GAANNNNNNNTTGG 2 cut(s) 796, 828
AloI GAACNNNNNNTCC 2 cut(s) 107, 139
AluBI AGCT 8 cut(s) 359, 635, 941, 985, 1361, 1426, 1607, 1652
AluI AGCT 8 cut(s) 359, 635, 941, 985, 1361, 1426, 1607, 1652
Alw26I GTCTC 5 cut(s) 112, 203, 955, 1471, 1649
AlwI GGATC 4 cut(s) 110, 123, 701, 1286
Ama87I CYCGRG 1 cut(s) 1133
Aor13HI TCCGGA 1 cut(s) 1398
AoxI GGCC 3 cut(s) 913, 1005, 1518
ApeKI GCWGC 1 cut(s) 1604
ApoI RAATTY 3 cut(s) 249, 301, 493
ArsI GACNNNNNNTTYG 2 cut(s) 1449, 1481
AseI ATTAAT 1 cut(s) 567
Asp700I GAANNNNTTC 1 cut(s) 728
AspS9I GGNCC 2 cut(s) 913, 1335
AsuC2I CCSGG 2 cut(s) 1134, 1135
AsuHPI GGTGA 3 cut(s) 1418, 1444, 1679
AsuII TTCGAA 2 cut(s) 59, 1282
AvaI CYCGRG 1 cut(s) 1133
AvaII GGWCC 1 cut(s) 1335
BaeGI GKGCMC 2 cut(s) 1388, 1441
BalI TGGCCA 1 cut(s) 1007
BamHI GGATCC 1 cut(s) 115
BanI GGYRCC 2 cut(s) 1385, 1438
BarI GAAGNNNNNNTAC 2 cut(s) 1182, 1214
BbvI GCAGC 1 cut(s) 1616
BccI CCATC 3 cut(s) 928, 997, 1688
BceAI ACGGC 2 cut(s) 355, 1533
BciT130I CCWGG 2 cut(s) 230, 1443
BclI TGATCA 3 cut(s) 138, 337, 1311
BcnI CCSGG 2 cut(s) 1134, 1135
BcoDI GTCTC 5 cut(s) 112, 203, 955, 1471, 1649
BfaI CTAG 1 cut(s) 986
BfmI CTRYAG 2 cut(s) 1556, 1614
BglII AGATCT 1 cut(s) 1041
BisI GCNGC 1 cut(s) 1605
BlsI GCNGC 1 cut(s) 1606
Bme1390I CCNGG 4 cut(s) 230, 1134, 1135, 1443
Bme18I GGWCC 1 cut(s) 1335
BmeT110I CYCGRG 1 cut(s) 1133
BmgT120I GGNCC 2 cut(s) 913, 1335
BmiI GGNNCC 4 cut(s) 117, 914, 1387, 1440
BmrFI CCNGG 4 cut(s) 230, 1134, 1135, 1443
BmsI GCATC 2 cut(s) 357, 1492
BplI GAGNNNNNCTC 2 cut(s) 1281, 1313
BpmI CTGGAG 3 cut(s) 69, 1453, 1569
Bpu10I CCTNAGC 1 cut(s) 463
Bpu14I TTCGAA 2 cut(s) 59, 1282
BpuEI CTTGAG 1 cut(s) 1066
BpuMI CCSGG 2 cut(s) 1134, 1135
Bsa29I ATCGAT 1 cut(s) 144
BsaAI YACGTR 1 cut(s) 625
BsaBI GATNNNNATC 1 cut(s) 1595
BsaJI CCNNGG 5 cut(s) 229, 371, 521, 549, 1133
BsaWI WCCGGW 1 cut(s) 1398
BsaXI ACNNNNNCTCC 2 cut(s) 1567, 1597
Bsc4I CCNNNNNNNGG 2 cut(s) 169, 1551
Bse118I RCCGGY 1 cut(s) 1551
Bse1I ACTGG 2 cut(s) 1250, 1463
Bse3DI GCAATG 1 cut(s) 784
Bse8I GATNNNNATC 1 cut(s) 1595
BseAI TCCGGA 1 cut(s) 1398
BseBI CCWGG 2 cut(s) 230, 1443
BseCI ATCGAT 1 cut(s) 144
BseDI CCNNGG 5 cut(s) 229, 371, 521, 549, 1133
BseGI GGATG 5 cut(s) 157, 223, 579, 1008, 1216
BseJI GATNNNNATC 1 cut(s) 1595
BseLI CCNNNNNNNGG 2 cut(s) 169, 1551
BseMI GCAATG 1 cut(s) 784
BseMII CTCAG 2 cut(s) 374, 477
BseNI ACTGG 2 cut(s) 1250, 1463
BseRI GAGGAG 1 cut(s) 968
BseSI GKGCMC 2 cut(s) 1388, 1441
BseXI GCAGC 1 cut(s) 1616
BseYI CCCAGC 1 cut(s) 1361
Bsh1236I CGCG 2 cut(s) 205, 1343
BshFI GGCC 3 cut(s) 915, 1007, 1520
BshNI GGYRCC 2 cut(s) 1385, 1438
BshVI ATCGAT 1 cut(s) 144
BsiHKCI CYCGRG 1 cut(s) 1133
BsiSI CCGG 3 cut(s) 1134, 1399, 1552
BslFI GGGAC 1 cut(s) 1339
BslI CCNNNNNNNGG 2 cut(s) 169, 1551
BsmAI GTCTC 5 cut(s) 112, 203, 955, 1471, 1649
BsmBI CGTCTC 1 cut(s) 1649
BsmFI GGGAC 1 cut(s) 1339
BsmI GAATGC 1 cut(s) 1625
BsnI GGCC 3 cut(s) 915, 1007, 1520
BsoBI CYCGRG 1 cut(s) 1133
Bsp119I TTCGAA 2 cut(s) 59, 1282
Bsp1286I GDGCHC 2 cut(s) 1388, 1441
Bsp13I TCCGGA 1 cut(s) 1398
BspANI GGCC 3 cut(s) 915, 1007, 1520
BspCNI CTCAG 2 cut(s) 373, 476
BspDI ATCGAT 1 cut(s) 144
BspEI TCCGGA 1 cut(s) 1398
BspFNI CGCG 2 cut(s) 205, 1343
BspLI GGNNCC 4 cut(s) 117, 914, 1387, 1440
BspPI GGATC 4 cut(s) 110, 123, 701, 1286
BspQI GCTCTTC 2 cut(s) 414, 1416
BspT104I TTCGAA 2 cut(s) 59, 1282
BspT107I GGYRCC 2 cut(s) 1385, 1438
BsrDI GCAATG 1 cut(s) 784
BsrFI RCCGGY 1 cut(s) 1551
BsrI ACTGG 2 cut(s) 1250, 1463
BssAI RCCGGY 1 cut(s) 1551
BssECI CCNNGG 5 cut(s) 229, 371, 521, 549, 1133
Bst2UI CCWGG 2 cut(s) 230, 1443
Bst4CI ACNGT 5 cut(s) 260, 391, 879, 1330, 1560
Bst6I CTCTTC 4 cut(s) 414, 1032, 1124, 1416
BstBAI YACGTR 1 cut(s) 625
BstBI TTCGAA 2 cut(s) 59, 1282
BstC8I GCNNGC 2 cut(s) 917, 1307
BstDEI CTNAG 3 cut(s) 360, 463, 926
BstDSI CCRYGG 1 cut(s) 371
BstF5I GGATG 5 cut(s) 157, 223, 579, 1008, 1216
BstFNI CGCG 2 cut(s) 205, 1343
BstMAI GTCTC 5 cut(s) 112, 203, 955, 1471, 1649
BstNI CCWGG 2 cut(s) 230, 1443
BstNSI RCATGY 4 cut(s) 601, 675, 720, 1481
BstSCI CCNGG 4 cut(s) 228, 1132, 1133, 1441
BstSFI CTRYAG 2 cut(s) 1556, 1614
BstSLI GKGCMC 2 cut(s) 1388, 1441
BstUI CGCG 2 cut(s) 205, 1343
BstV1I GCAGC 1 cut(s) 1616
BstX2I RGATCY 4 cut(s) 115, 706, 1041, 1291
BstXI CCANNNNNNTGG 2 cut(s) 293, 1528
BstYI RGATCY 4 cut(s) 115, 706, 1041, 1291
Bsu15I ATCGAT 1 cut(s) 144
BsuRI GGCC 3 cut(s) 915, 1007, 1520
BsuTUI ATCGAT 1 cut(s) 144
BtgI CCRYGG 1 cut(s) 371
BtgZI GCGATG 1 cut(s) 1604
BtsCI GGATG 5 cut(s) 157, 223, 579, 1008, 1216
BtsI GCAGTG 1 cut(s) 753
BtsIMutI CAGTG 4 cut(s) 753, 1257, 1335, 1565
Cac8I GCNNGC 2 cut(s) 917, 1307
Cfr10I RCCGGY 1 cut(s) 1551
Cfr13I GGNCC 2 cut(s) 913, 1335
Cfr9I CCCGGG 1 cut(s) 1133
ClaI ATCGAT 1 cut(s) 144
CseI GACGC 2 cut(s) 1106, 1332
Csp6I GTAC 5 cut(s) 626, 643, 992, 1258, 1575
CviAII CATG 9 cut(s) 93, 349, 598, 672, 717, 1171, 1207, 1478, 1600
CviQI GTAC 5 cut(s) 626, 643, 992, 1258, 1575
DdeI CTNAG 3 cut(s) 360, 463, 926
DrdI GACNNNNNNGTC 1 cut(s) 1342
DseDI GACNNNNNNGTC 1 cut(s) 1342
EaeI YGGCCR 2 cut(s) 1005, 1518
Eam1104I CTCTTC 4 cut(s) 414, 1032, 1124, 1416
EarI CTCTTC 4 cut(s) 414, 1032, 1124, 1416
Eco32I GATATC 1 cut(s) 90
Eco47I GGWCC 1 cut(s) 1335
Eco57I CTGAAG 3 cut(s) 836, 1166, 1265
Eco88I CYCGRG 1 cut(s) 1133
EcoO109I RGGNCCY 1 cut(s) 913
EcoRII CCWGG 2 cut(s) 228, 1441
EcoRV GATATC 1 cut(s) 90
EcoT22I ATGCAT 1 cut(s) 1507
Esp3I CGTCTC 1 cut(s) 1649
FaeI CATG 9 cut(s) 96, 352, 601, 675, 720, 1174, 1210, 1481, 1603
FaqI GGGAC 1 cut(s) 1339
FatI CATG 9 cut(s) 92, 348, 597, 671, 716, 1170, 1206, 1477, 1599
FbaI TGATCA 3 cut(s) 138, 337, 1311
Fnu4HI GCNGC 1 cut(s) 1605
FokI GGATG 5 cut(s) 164, 230, 586, 1015, 1223
Fsp4HI GCNGC 1 cut(s) 1605
FspBI CTAG 1 cut(s) 986
GluI GCNGC 1 cut(s) 1605
GsaI CCCAGC 1 cut(s) 1365
GsuI CTGGAG 3 cut(s) 69, 1453, 1569
HaeIII GGCC 3 cut(s) 915, 1007, 1520
HapII CCGG 3 cut(s) 1134, 1399, 1552
HgaI GACGC 2 cut(s) 1106, 1332
Hin1II CATG 9 cut(s) 96, 352, 601, 675, 720, 1174, 1210, 1481, 1603
HincII GTYRAC 2 cut(s) 669, 1224
HindII GTYRAC 2 cut(s) 669, 1224
HinfI GANTC 4 cut(s) 534, 845, 856, 1371
HpaI GTTAAC 1 cut(s) 669
HpaII CCGG 3 cut(s) 1134, 1399, 1552
HphI GGTGA 3 cut(s) 1418, 1444, 1679
Hpy166II GTNNAC 4 cut(s) 669, 994, 1224, 1335
Hpy188III TCNNGA 6 cut(s) 113, 136, 149, 465, 1045, 1399
Hpy8I GTNNAC 4 cut(s) 669, 994, 1224, 1335
Hpy99I CGWCG 2 cut(s) 1348, 1644
HpyAV CCTTC 4 cut(s) 173, 860, 1316, 1561
HpyCH4III ACNGT 5 cut(s) 260, 391, 879, 1330, 1560
HpyCH4IV ACGT 8 cut(s) 55, 132, 179, 486, 624, 990, 1226, 1256
HpyCH4V TGCA 4 cut(s) 272, 758, 1170, 1505
HpyF3I CTNAG 3 cut(s) 360, 463, 926
HpySE526I ACGT 8 cut(s) 55, 132, 179, 486, 624, 990, 1226, 1256
Hsp92II CATG 9 cut(s) 96, 352, 601, 675, 720, 1174, 1210, 1481, 1603
Kpn2I TCCGGA 1 cut(s) 1398
Ksp22I TGATCA 3 cut(s) 138, 337, 1311
KspAI GTTAAC 1 cut(s) 669
LguI GCTCTTC 2 cut(s) 414, 1416
LmnI GCTCC 2 cut(s) 632, 868
Lsp1109I GCAGC 1 cut(s) 1616
LweI GCATC 2 cut(s) 357, 1492
MaeI CTAG 1 cut(s) 986
MaeII ACGT 8 cut(s) 55, 132, 179, 486, 624, 990, 1226, 1256
MaeIII GTNAC 2 cut(s) 1252, 1406
MfeI CAATTG 1 cut(s) 733
MflI RGATCY 4 cut(s) 115, 706, 1041, 1291
MhlI GDGCHC 2 cut(s) 1388, 1441
MlsI TGGCCA 1 cut(s) 1007
MluCI AATT 5 cut(s) 249, 301, 493, 733, 1111
MluI ACGCGT 1 cut(s) 1341
MluNI TGGCCA 1 cut(s) 1007
Mox20I TGGCCA 1 cut(s) 1007
Mph1103I ATGCAT 1 cut(s) 1507
MroI TCCGGA 1 cut(s) 1398
MroXI GAANNNNTTC 1 cut(s) 728
MscI TGGCCA 1 cut(s) 1007
MseI TTAA 6 cut(s) 567, 582, 668, 825, 1031, 1693
MslI CAYNNNNRTG 1 cut(s) 1001
Msp20I TGGCCA 1 cut(s) 1007
MspI CCGG 3 cut(s) 1134, 1399, 1552
MspR9I CCNGG 4 cut(s) 230, 1134, 1135, 1443
MunI CAATTG 1 cut(s) 733
Mva1269I GAATGC 1 cut(s) 1625
MvaI CCWGG 2 cut(s) 230, 1443
MvnI CGCG 2 cut(s) 205, 1343
NciI CCSGG 2 cut(s) 1134, 1135
NlaIII CATG 9 cut(s) 96, 352, 601, 675, 720, 1174, 1210, 1481, 1603
NlaIV GGNNCC 4 cut(s) 117, 914, 1387, 1440
NmeAIII GCCGAG 1 cut(s) 574
NmuCI GTSAC 2 cut(s) 1252, 1406
NsiI ATGCAT 1 cut(s) 1507
NspI RCATGY 4 cut(s) 601, 675, 720, 1481
NspV TTCGAA 2 cut(s) 59, 1282
PciI ACATGT 3 cut(s) 597, 671, 1477
PciSI GCTCTTC 2 cut(s) 414, 1416
PcsI WCGNNNNNNNCGW 1 cut(s) 1645
PctI GAATGC 1 cut(s) 1625
PdmI GAANNNNTTC 1 cut(s) 728
PfeI GAWTC 4 cut(s) 534, 845, 856, 1371
PkrI GCNGC 1 cut(s) 1606
Ppu21I YACGTR 1 cut(s) 625
PscI ACATGT 3 cut(s) 597, 671, 1477
PshBI ATTAAT 1 cut(s) 567
PsiI TTATAA 1 cut(s) 776
Psp1406I AACGTT 2 cut(s) 55, 179
Psp6I CCWGG 2 cut(s) 228, 1441
PspFI CCCAGC 1 cut(s) 1361
PspGI CCWGG 2 cut(s) 228, 1441
PspN4I GGNNCC 4 cut(s) 117, 914, 1387, 1440
PspPI GGNCC 2 cut(s) 913, 1335
PsuI RGATCY 4 cut(s) 115, 706, 1041, 1291
RsaI GTAC 5 cut(s) 627, 644, 993, 1259, 1576
RsaNI GTAC 5 cut(s) 626, 643, 992, 1258, 1575
RseI CAYNNNNRTG 1 cut(s) 1001
SapI GCTCTTC 2 cut(s) 414, 1416
SaqAI TTAA 6 cut(s) 567, 582, 668, 825, 1031, 1693
SatI GCNGC 1 cut(s) 1605
Sau96I GGNCC 2 cut(s) 913, 1335
ScrFI CCNGG 4 cut(s) 230, 1134, 1135, 1443
SduI GDGCHC 2 cut(s) 1388, 1441
SfaNI GCATC 2 cut(s) 357, 1492
SfcI CTRYAG 2 cut(s) 1556, 1614
SfuI TTCGAA 2 cut(s) 59, 1282
SinI GGWCC 1 cut(s) 1335
SmaI CCCGGG 1 cut(s) 1135
SmiMI CAYNNNNRTG 1 cut(s) 1001
SmlI CTYRAG 1 cut(s) 1045
SmoI CTYRAG 1 cut(s) 1045
Sse9I AATT 5 cut(s) 249, 301, 493, 733, 1111
SspI AATATT 2 cut(s) 81, 837
SspMI CTAG 1 cut(s) 986
StyD4I CCNGG 4 cut(s) 228, 1132, 1133, 1441
TaaI ACNGT 5 cut(s) 260, 391, 879, 1330, 1560
TaiI ACGT 8 cut(s) 58, 135, 182, 489, 627, 993, 1229, 1259
TaqI TCGA 8 cut(s) 59, 144, 532, 745, 1282, 1299, 1346, 1431
TasI AATT 5 cut(s) 249, 301, 493, 733, 1111
TfiI GAWTC 4 cut(s) 534, 845, 856, 1371
Tru1I TTAA 6 cut(s) 567, 582, 668, 825, 1031, 1693
Tru9I TTAA 6 cut(s) 567, 582, 668, 825, 1031, 1693
TscAI CASTG 4 cut(s) 760, 1257, 1335, 1565
TseFI GTSAC 2 cut(s) 1252, 1406
TseI GCWGC 1 cut(s) 1604
Tsp45I GTSAC 2 cut(s) 1252, 1406
TspDTI ATGAA 9 cut(s) 365, 470, 804, 810, 986, 992, 1029, 1187, 1607
TspGWI ACGGA 3 cut(s) 230, 881, 1619
TspMI CCCGGG 1 cut(s) 1133
TspRI CASTG 4 cut(s) 760, 1257, 1335, 1565
VpaK11BI GGWCC 1 cut(s) 1335
VspI ATTAAT 1 cut(s) 567
XapI RAATTY 3 cut(s) 249, 301, 493
XceI RCATGY 4 cut(s) 601, 675, 720, 1481
XmaI CCCGGG 1 cut(s) 1133
XmnI GAANNNNTTC 1 cut(s) 728
XspI CTAG 1 cut(s) 986
Zsp2I ATGCAT 1 cut(s) 1507
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.