Rw2G022800

Cyclic nucleotide-gated ion channel 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000119
Physical Location & Seq
Forward (+)
32696771 .. 32707275
10505 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G022800.1

Sequence Viewer

Length: 2076 bp
ATGAAAACAAAAACGATCATTATGATGGAGGATAAGCAAGATCCAGCTCAACATAGCCATACACAAGGTGACTCAGACCAAAAAAAAAAAAGCCATACACAAGGTGACGCTGCAAATCCAAGTGGTGGTAAGGATAAGAATTCCTCATGTGTGATTGCAAACGAACGTACCCATGAACAACATTTACAAACGAACGAACAGAACAAGAAATTACTTCAGAAACTTCGAGGTACAAGCTGGGATACCATACTTGTAATCTCATGTGTGATTGCAGTCTCAGTTGATCCTTTGTTCTTTTACCTTCCGATCATTAATGAGGAAATCAAGTGCCTTGGAGTGGACAAAAATTTGAGGACTGCTGCTCTTCTTTTGCGCGCGATCACTGATATTGCTTTCATCCTGAACATTTTCTATCAAATTAAAGATGTCATCGAAAATGTACAGTCTAGAGCAAAGTCGATCAATAATAATAGTAAGAATAATGGAGAAGGAAAGCAGGTTCCACAATCCAAACAAGATGCGAGAATTCGGTTCGCTAAGATAGCTCGAAAGATGCCATGGCTCTCAATTACAATTGACATTCTTGCGGTTCTCCCCATCCCACAAGTGCTAGTGGCTGGTTTCTTCAAAATGAGAAGCTCCAAATATCTGGGCCGGAGAAAGATTATTAATGTCTTTCTTATCGCCCAATATTTGCCACGGATTTATCGTATGCATCACTCATCAATGAAACTTAAGCAAAAGAAGGGACCATGGATTAGGGGTGCATTATATTTTTTCTTGTACATCCTGGCCAGTCATGTGCTGGGAGCTTTCTGGTATTTTTTCTCTATTCAACGAGAGACATCATGTTGGCACCGAGCATGTATGAACCATAGTACAAGTGAATGTAATTTTTACTGCAATGAAGACATTTCTTTTAGGAATATGACATTCATTAGTTCTCTAGATGAATTTTGTCTTGTTGATGTTCCGGCAAATGTGACAGCACCGTTTGATTTCGGAATATTTCTTGATTCCCTTAAGAGTGGTAACATAGGGCAAATAAATTTTGCAAAGAAGTTTTTTTACTCTTTTTGGTGGGGACTACGAAATCTTAGTAACTTTGGGACAAACTTGGAAACAAGTACCTATGTGTTGGAAAACTGCTTTGCGATTCTTATATCTATTGTCGGCTTGCTTCTTTTCCTATATCTCATTGGAAATGTGCAGACATTTATATCGCTGGAAACTGAAAAATCAGAGGAGAGAAGAAAAAAGCTACGCTCGACAGAAATGGATATAGAAGTGTGGATGGAGAGAAATGGTCTGAATAAAGAGATAAAGAACGAAATCAATAAAATTGTAAAAAAAAAATCAGAGAAAATCAAAGATGCTGATATGGAGAACGTATTCTATATTCTCCCAAATGCAACCCGAATTTCTCTAAAGCGAGCTCTCTGCATGGAAATACTAAAGAAGGTTCCCTTGAGGCTTAAGGCTATGGAAAAGAAACCAGACCAAGAGAATAAAGAGACCATACAAGAAAAAGTGTTGACGAGCATTTGTGACTCTCTGAAGCCAGTGATGTACCCGGATAATAGCTTTGTTTTCCGCATGGGGGAACCACTTGATCGCATGCTTTTAATTACAGAAGGTCTAGTATGGGCATATAACACCACCTCTAGTAGTATTAGTCAAGGTGGAGAAGCAGGTATAGGTGCCATTTGTCTTGGGAAAGGTGCATATTACGGAGATGAACTTGTGAGTTGGGCATCAGCTAGGCTGCTCGACTCTTTGGAAAACGTTCCTATGCTGAAAGCAAATGTCAAGTGTCATACAAAAATAGAAGGATTCGTTCTTACGGCCAAGGATTTGAACGCTATAGTCCACAAATACAAATTCTTTTGGGATTTCAGTGATTCTCAGATAAGAAAGTTGGCTGCAATTCAAACCATTCAAAAAGCAGCTCGTCAATTGACGCTGCGGTCTAAGAAAGATAAAAAAAAGGTCCTGCAACCGGATTCTCCACCGGATTCTCTAGTAGTTGATGTTCCATCAGAGGACGCAACAACAGCTAGCAGTCAAGTCCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
Ion_trans

Protein Analysis

691

Amino Acids

78.71

Weight (kDa)

9.29

Isoelectric Point (pI)

45.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ion_trans PF00520 191 - 414 6.6e-07 Ion transport protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000119)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g10570 FvH4_4g36390 FvH4_7g31840 FvH4_7g31840 FvH4_7g31850 FvH4_7g31850 FvH4_7g31861 FvH4_7g31861 FvH4_7g31950
malus_domestica MD01G1054600.v1.1 MD01G1221100.v1.1 MD01G1221400.v1.1 MD01G1221600.v1.1 MD01G1221800.v1.1 MD01G1222000.v1.1 MD01G1222100.v1.1 MD07G1140800.v1.1 MD07G1291200.v1.1 MD07G1291400.v1.1 MD07G1291500.v1.1 MD07G1291700.v1.1 MD07G1292500.v1.1 MD07G1292700.v1.1 MD07G1292800.v1.1 MD07G1292900.v1.1 MD08G1241600.v1.1 MD11G1083800.v1.1 MD15G1431600.v1.1
prunus_persica Prupe.1G017800_v2.0.a1 Prupe.1G017800_v2.0.a1 Prupe.1G162800_v2.0.a1 Prupe.2G312500_v2.0.a1 Prupe.2G312500_v2.0.a1 Prupe.2G312700_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092800_v2.0.a1 Prupe.4G092800_v2.0.a1 Prupe.6G142800_v2.0.a1 Prupe.6G142800_v2.0.a1 Prupe.6G143000_v2.0.a1 Prupe.6G143100_v2.0.a1 Prupe.6G143100_v2.0.a1 Prupe.6G143100_v2.0.a1 Prupe.6G143100_v2.0.a1
pyrus_communis pycom01g23050 pycom01g23070 pycom01g23090 pycom01g23100 pycom01g23110 pycom01g23120 pycom01g23170 pycom07g26410 pycom07g26440 pycom07g26470 pycom07g26480 pycom07g26490 pycom07g26500 pycom07g26510 pycom07g26530 pycom07g26540 pycom07g26570 pycom07g26580 pycom07g26590 pycom08g20940
rosa_chinensis RchiOBHm_Chr1g0324571 RchiOBHm_Chr1g0336181 RchiOBHm_Chr1g0381061 RchiOBHm_Chr1g0381091 RchiOBHm_Chr1g0381121 RchiOBHm_Chr1g0381131 RchiOBHm_Chr1g0381141 RchiOBHm_Chr1g0381161 RchiOBHm_Chr1g0381211 RchiOBHm_Chr2g0110361 RchiOBHm_Chr2g0120231 RchiOBHm_Chr3g0486281 RchiOBHm_Chr3g0486311 RchiOBHm_Chr3g0486401 RchiOBHm_Chr4g0409511 RchiOBHm_Chr4g0409521 RchiOBHm_Chr5g0017271 RchiOBHm_Chr5g0048781 RchiOBHm_Chr5g0048811
rosa_laevigata RLG00000002401 RLG00000008559 RLG00000017798 RLG00000017799 RLG00000023082 RLG00000023088 RLG00000026230 RLG00000026238 RLG00000026240 RLG00000026241 RLG00000026242 RLG00000026243 RLG00000026244 RLG00000026246 RLG00000026247 RLG00000026248 RLG00000029876 RLG00000030189 RLG00000034579
rosa_multiflora Rmu_co8101374.1_g000001 Rmu_co8309149.1_g000001 Rmu_co8450063.1_g000001 Rmu_sc0000441.1_g000094 Rmu_sc0000479.1_g000005 Rmu_sc0000647.1_g000006 Rmu_sc0001393.1_g000031 Rmu_sc0001423.1_g000037 Rmu_sc0001616.1_g000015 Rmu_sc0001857.1_g000011 Rmu_sc0002183.1_g000013 Rmu_sc0002183.1_g000028 Rmu_sc0002183.1_g000030 Rmu_sc0002688.1_g000003 Rmu_sc0002688.1_g000020 Rmu_sc0003801.1_g000003 Rmu_sc0003801.1_g000004 Rmu_sc0004125.1_g000006 Rmu_sc0004494.1_g000005 Rmu_sc0004494.1_g000006 Rmu_sc0004494.1_g000008 Rmu_sc0004494.1_g000009 Rmu_sc0005842.1_g000004 Rmu_sc0006872.1_g000001 Rmu_sc0006872.1_g000005 Rmu_sc0006872.1_g000009 Rmu_sc0006872.1_g000010 Rmu_sc0008053.1_g000026
rosa_roxburghii Rroxscaffold_159G00432960 Rroxscaffold_1G00033060 Rroxscaffold_2G00123150 Rroxscaffold_2G00123170 Rroxscaffold_2G00133500 Rroxscaffold_2G00133550 Rroxscaffold_4G00278650 Rroxscaffold_4G00278750 Rroxscaffold_4G00278790 Rroxscaffold_4G00278800 Rroxscaffold_4G00278810 Rroxscaffold_4G00325390 Rroxscaffold_5G00354010 Rroxscaffold_6G00395890 Rroxscaffold_6G00395900 Rroxscaffold_6G00395920
rosa_rugosa Rorug01G0046900.1 Rorug01G0427000 Rorug01G0427100 Rorug01G0427200 Rorug01G0427300 Rorug01G0427400 Rorug01G0427500 Rorug01G0427500 Rorug01G0427600 Rorug01G0427600 Rorug01G0427700 Rorug01G0427700 Rorug01G0427800 Rorug01G0428700 Rorug02G0166400 Rorug02G0166500 Rorug02G0166500 Rorug02G0166600 Rorug02G0166600 Rorug02G0166700 Rorug02G0167300 Rorug02G0167300 Rorug02G0167300 Rorug03G0224600 Rorug03G0224600 Rorug04G0094400 Rorug05G0241100 Rorug05G0241200
rosa_samantha Rh1BG407700 Rh1BG407900 Rh1BG409000 Rh1CG064700 Rh1CG420900 Rh1CG421000 Rh1CG421200 Rh1CG421300 Rh1CG421800 Rh1CG421900 Rh1CG422000 Rh1CG422200 Rh1CG422300 Rh1CG423600 Rh1DG437600 Rh1DG438900 Rh1DG439000 Rh1DG440300 Rh2AG219100 Rh2CG220700 Rh2DG225300 Rh3AG274400 Rh3AG274600 Rh3CG308900 Rh3DG305800 Rh4AG156400 Rh5AG129000 Rh5AG320900 Rh5CG139600
rosa_wichuraiana Rw1G005300 Rw1G039280 Rw1G039300 Rw1G039310 Rw1G039320 Rw1G039330 Rw1G039340 Rw1G039360 Rw1G039390 Rw2G016910 Rw2G022800 Rw3G024330 Rw3G024340 Rw3G024350 Rw3G024400 Rw4G012810 Rw5G030120 Rw5G030280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1966
Acc36I ACCTGC 2 cut(s) 487, 1682
AccB1I GGYRCC 2 cut(s) 855, 1700
AccB7I CCANNNNNTGG 1 cut(s) 125
AccII CGCG 2 cut(s) 375, 377
AciI CCGC 3 cut(s) 587, 1594, 1966
AclI AACGTT 1 cut(s) 1785
AclWI GGATC 2 cut(s) 35, 278
AcoI YGGCCR 2 cut(s) 792, 1845
AcsI RAATTY 7 cut(s) 139, 346, 525, 953, 1048, 1419, 1880
AcuI CTGAAG 2 cut(s) 200, 1577
AdeI CACNNNGTG 2 cut(s) 68, 104
AfaI GTAC 7 cut(s) 169, 232, 441, 785, 880, 1129, 1571
AfiI CCNNNNNNNGG 4 cut(s) 125, 337, 1600, 1999
AflII CTTAAG 3 cut(s) 734, 1022, 1475
AgsI TTSAA 5 cut(s) 628, 836, 1858, 1931, 1940
AjnI CCWGG 1 cut(s) 789
AloI GAACNNNNNNTCC 2 cut(s) 483, 515
Alw21I GWGCWC 1 cut(s) 1438
Alw26I GTCTC 3 cut(s) 280, 836, 1508
AlwI GGATC 2 cut(s) 35, 278
AoxI GGCC 3 cut(s) 652, 792, 1845
ApeKI GCWGC 6 cut(s) 110, 359, 1765, 1922, 1946, 1963
ApoI RAATTY 7 cut(s) 139, 346, 525, 953, 1048, 1419, 1880
AseI ATTAAT 2 cut(s) 312, 669
Asp700I GAANNNNTTC 3 cut(s) 407, 1391, 1785
AspLEI GCGC 2 cut(s) 375, 377
AspS9I GGNCC 3 cut(s) 652, 749, 1990
AsuC2I CCSGG 1 cut(s) 1574
AsuHPI GGTGA 2 cut(s) 80, 116
AsuNHI GCTAGC 1 cut(s) 2057
AvaII GGWCC 2 cut(s) 749, 1990
BaeI ACNNNNGTAYC 2 cut(s) 151, 184
BalI TGGCCA 1 cut(s) 794
BanI GGYRCC 2 cut(s) 855, 1700
BanII GRGCYC 1 cut(s) 1438
BarI GAAGNNNNNNTAC 2 cut(s) 1052, 1084
BbsI GAAGAC 1 cut(s) 915
Bbv12I GWGCWC 1 cut(s) 1438
BbvI GCAGC 6 cut(s) 97, 346, 1752, 1909, 1950, 1958
BccI CCATC 4 cut(s) 19, 605, 1288, 2044
BceAI ACGGC 1 cut(s) 1860
BcgI CGANNNNNNTGC 2 cut(s) 1422, 1456
BciT130I CCWGG 1 cut(s) 791
BciVI GTATCC 1 cut(s) 235
BcnI CCSGG 1 cut(s) 1574
BcoDI GTCTC 3 cut(s) 280, 836, 1508
BfaI CTAG 8 cut(s) 447, 611, 947, 1640, 1665, 1761, 2021, 2058
BfmI CTRYAG 1 cut(s) 1863
BfrI CTTAAG 3 cut(s) 734, 1022, 1475
BfuAI ACCTGC 2 cut(s) 487, 1682
BfuI GTATCC 1 cut(s) 235
BisI GCNGC 6 cut(s) 111, 360, 1766, 1923, 1947, 1964
BlsI GCNGC 6 cut(s) 112, 361, 1767, 1924, 1948, 1965
Bme1390I CCNGG 2 cut(s) 791, 1574
Bme18I GGWCC 2 cut(s) 749, 1990
BmgT120I GGNCC 3 cut(s) 652, 749, 1990
BmiI GGNNCC 6 cut(s) 501, 750, 857, 1464, 1605, 1702
BmrFI CCNGG 2 cut(s) 791, 1574
BmsI GCATC 5 cut(s) 508, 543, 724, 1363, 1763
BmtI GCTAGC 1 cut(s) 2061
BpiI GAAGAC 1 cut(s) 915
BpuEI CTTGAG 1 cut(s) 1489
BpuMI CCSGG 1 cut(s) 1574
BsaI GGTCTC 1 cut(s) 1508
BsaJI CCNNGG 5 cut(s) 331, 557, 698, 752, 1848
BsaWI WCCGGW 2 cut(s) 1999, 2011
Bsc4I CCNNNNNNNGG 4 cut(s) 125, 337, 1600, 1999
Bse1I ACTGG 2 cut(s) 795, 1562
Bse3DI GCAATG 1 cut(s) 910
BseBI CCWGG 1 cut(s) 791
BseDI CCNNGG 5 cut(s) 331, 557, 698, 752, 1848
BseGI GGATG 4 cut(s) 396, 597, 786, 1299
BseLI CCNNNNNNNGG 4 cut(s) 125, 337, 1600, 1999
BseMI GCAATG 1 cut(s) 910
BseMII CTCAG 3 cut(s) 87, 291, 1919
BseNI ACTGG 2 cut(s) 795, 1562
BsePI GCGCGC 1 cut(s) 373
BseRI GAGGAG 1 cut(s) 1259
BseXI GCAGC 6 cut(s) 97, 346, 1752, 1909, 1950, 1958
BseYI CCCAGC 2 cut(s) 237, 805
BsgI GTGCAG 1 cut(s) 1229
Bsh1236I CGCG 2 cut(s) 375, 377
BshFI GGCC 3 cut(s) 654, 794, 1847
BshNI GGYRCC 2 cut(s) 855, 1700
BsiHKAI GWGCWC 1 cut(s) 1438
BsiSI CCGG 5 cut(s) 655, 974, 1574, 2000, 2012
BslFI GGGAC 4 cut(s) 762, 1098, 1123, 2054
BslI CCNNNNNNNGG 4 cut(s) 125, 337, 1600, 1999
BsmAI GTCTC 3 cut(s) 280, 836, 1508
BsmFI GGGAC 4 cut(s) 762, 1098, 1123, 2054
BsnI GGCC 3 cut(s) 654, 794, 1847
Bso31I GGTCTC 1 cut(s) 1508
Bsp1286I GDGCHC 1 cut(s) 1438
Bsp1407I TGTACA 2 cut(s) 439, 783
Bsp143I GATC 7 cut(s) 15, 40, 283, 306, 378, 459, 1612
Bsp19I CCATGG 2 cut(s) 557, 752
BspACI CCGC 3 cut(s) 587, 1594, 1966
BspANI GGCC 3 cut(s) 654, 794, 1847
BspCNI CTCAG 3 cut(s) 86, 290, 1918
BspFNI CGCG 2 cut(s) 375, 377
BspLI GGNNCC 6 cut(s) 501, 750, 857, 1464, 1605, 1702
BspMI ACCTGC 2 cut(s) 487, 1682
BspOI GCTAGC 1 cut(s) 2061
BspPI GGATC 2 cut(s) 35, 278
BspQI GCTCTTC 1 cut(s) 369
BspT107I GGYRCC 2 cut(s) 855, 1700
BspTI CTTAAG 3 cut(s) 734, 1022, 1475
BspTNI GGTCTC 1 cut(s) 1508
BsrDI GCAATG 1 cut(s) 910
BsrGI TGTACA 2 cut(s) 439, 783
BsrI ACTGG 2 cut(s) 795, 1562
BssECI CCNNGG 5 cut(s) 331, 557, 698, 752, 1848
BssHII GCGCGC 1 cut(s) 373
BssMI GATC 7 cut(s) 15, 40, 283, 306, 378, 459, 1612
BssT1I CCWWGG 4 cut(s) 331, 557, 752, 1848
Bst2UI CCWGG 1 cut(s) 791
Bst4CI ACNGT 2 cut(s) 444, 993
Bst6I CTCTTC 1 cut(s) 369
BstAFI CTTAAG 3 cut(s) 734, 1022, 1475
BstAUI TGTACA 2 cut(s) 439, 783
BstC8I GCNNGC 5 cut(s) 375, 1178, 1434, 1619, 2059
BstDEI CTNAG 6 cut(s) 73, 277, 537, 1097, 1905, 1971
BstDSI CCRYGG 3 cut(s) 557, 698, 752
BstF5I GGATG 4 cut(s) 396, 597, 786, 1299
BstFNI CGCG 2 cut(s) 375, 377
BstHHI GCGC 2 cut(s) 375, 377
BstKTI GATC 7 cut(s) 18, 43, 286, 309, 381, 462, 1615
BstMAI GTCTC 3 cut(s) 280, 836, 1508
BstMBI GATC 7 cut(s) 15, 40, 283, 306, 378, 459, 1612
BstMWI GCNNNNNNNGC 2 cut(s) 542, 2054
BstNI CCWGG 1 cut(s) 791
BstNSI RCATGY 2 cut(s) 867, 1621
BstSCI CCNGG 2 cut(s) 789, 1572
BstSFI CTRYAG 1 cut(s) 1863
BstUI CGCG 2 cut(s) 375, 377
BstV1I GCAGC 6 cut(s) 97, 346, 1752, 1909, 1950, 1958
BstV2I GAAGAC 1 cut(s) 915
BstX2I RGATCY 1 cut(s) 40
BstXI CCANNNNNNTGG 1 cut(s) 649
BstYI RGATCY 1 cut(s) 40
BsuI GTATCC 1 cut(s) 235
BsuRI GGCC 3 cut(s) 654, 794, 1847
BtgI CCRYGG 3 cut(s) 557, 698, 752
BtsCI GGATG 4 cut(s) 396, 597, 786, 1299
BtsIMutI CAGTG 3 cut(s) 381, 1569, 1903
BveI ACCTGC 2 cut(s) 487, 1682
Cac8I GCNNGC 5 cut(s) 375, 1178, 1434, 1619, 2059
CfoI GCGC 2 cut(s) 375, 377
Cfr13I GGNCC 3 cut(s) 652, 749, 1990
CseI GACGC 3 cut(s) 116, 1969, 2054
Csp6I GTAC 7 cut(s) 168, 231, 440, 784, 879, 1128, 1570
CviQI GTAC 7 cut(s) 168, 231, 440, 784, 879, 1128, 1570
DdeI CTNAG 6 cut(s) 73, 277, 537, 1097, 1905, 1971
DpnI GATC 7 cut(s) 17, 42, 285, 308, 380, 461, 1614
DpnII GATC 7 cut(s) 15, 40, 283, 306, 378, 459, 1612
DraIII CACNNNGTG 2 cut(s) 68, 104
DrdI GACNNNNNNGTC 1 cut(s) 1966
DseDI GACNNNNNNGTC 1 cut(s) 1966
EaeI YGGCCR 2 cut(s) 792, 1845
Eam1104I CTCTTC 1 cut(s) 369
EarI CTCTTC 1 cut(s) 369
Ecl136II GAGCTC 1 cut(s) 1436
Eco130I CCWWGG 4 cut(s) 331, 557, 752, 1848
Eco24I GRGCYC 1 cut(s) 1438
Eco31I GGTCTC 1 cut(s) 1508
Eco47I GGWCC 2 cut(s) 749, 1990
Eco53kI GAGCTC 1 cut(s) 1436
Eco57I CTGAAG 2 cut(s) 200, 1577
EcoICRI GAGCTC 1 cut(s) 1436
EcoO109I RGGNCCY 1 cut(s) 1990
EcoRI GAATTC 2 cut(s) 139, 525
EcoRII CCWGG 1 cut(s) 789
EcoT14I CCWWGG 4 cut(s) 331, 557, 752, 1848
EcoT22I ATGCAT 1 cut(s) 717
EcoT38I GRGCYC 1 cut(s) 1438
ErhI CCWWGG 4 cut(s) 331, 557, 752, 1848
FalI AAGNNNNNCTT 2 cut(s) 1451, 1483
FaqI GGGAC 4 cut(s) 762, 1098, 1123, 2054
Fnu4HI GCNGC 6 cut(s) 111, 360, 1766, 1923, 1947, 1964
FokI GGATG 4 cut(s) 383, 584, 773, 1306
FriOI GRGCYC 1 cut(s) 1438
Fsp4HI GCNGC 6 cut(s) 111, 360, 1766, 1923, 1947, 1964
FspBI CTAG 8 cut(s) 447, 611, 947, 1640, 1665, 1761, 2021, 2058
GlaI GCGC 2 cut(s) 374, 376
GluI GCNGC 6 cut(s) 111, 360, 1766, 1923, 1947, 1964
GsaI CCCAGC 2 cut(s) 241, 809
HaeIII GGCC 3 cut(s) 654, 794, 1847
HapII CCGG 5 cut(s) 655, 974, 1574, 2000, 2012
HgaI GACGC 3 cut(s) 116, 1969, 2054
HhaI GCGC 2 cut(s) 375, 377
Hin6I GCGC 2 cut(s) 373, 375
HinP1I GCGC 2 cut(s) 373, 375
HincII GTYRAC 1 cut(s) 1536
HindII GTYRAC 1 cut(s) 1536
HinfI GANTC 9 cut(s) 71, 1016, 1156, 1550, 1772, 1833, 1901, 2003, 2015
HpaII CCGG 5 cut(s) 655, 974, 1574, 2000, 2012
HphI GGTGA 2 cut(s) 80, 116
Hpy166II GTNNAC 3 cut(s) 340, 1536, 1870
Hpy188III TCNNGA 4 cut(s) 400, 447, 947, 1013
Hpy8I GTNNAC 3 cut(s) 340, 1536, 1870
HpyAV CCTTC 6 cut(s) 311, 482, 739, 1453, 1628, 1823
HpyCH4III ACNGT 2 cut(s) 444, 993
HpyCH4IV ACGT 3 cut(s) 166, 1389, 1785
HpyF10VI GCNNNNNNNGC 2 cut(s) 542, 2054
HpyF3I CTNAG 6 cut(s) 73, 277, 537, 1097, 1905, 1971
HpySE526I ACGT 3 cut(s) 166, 1389, 1785
HspAI GCGC 2 cut(s) 373, 375
Kzo9I GATC 7 cut(s) 15, 40, 283, 306, 378, 459, 1612
LguI GCTCTTC 1 cut(s) 369
LmnI GCTCC 2 cut(s) 644, 809
Lsp1109I GCAGC 6 cut(s) 97, 346, 1752, 1909, 1950, 1958
LweI GCATC 5 cut(s) 508, 543, 724, 1363, 1763
MaeI CTAG 8 cut(s) 447, 611, 947, 1640, 1665, 1761, 2021, 2058
MaeII ACGT 3 cut(s) 166, 1389, 1785
MaeIII GTNAC 6 cut(s) 68, 104, 982, 1031, 1100, 1547
MalI GATC 7 cut(s) 17, 42, 285, 308, 380, 461, 1614
MboI GATC 7 cut(s) 15, 40, 283, 306, 378, 459, 1612
MboII GAAGA 4 cut(s) 356, 616, 920, 1263
MfeI CAATTG 2 cut(s) 573, 1955
MflI RGATCY 1 cut(s) 40
MhlI GDGCHC 1 cut(s) 1438
MlsI TGGCCA 1 cut(s) 794
MluNI TGGCCA 1 cut(s) 794
MlyI GAGTC 3 cut(s) 65, 1544, 1766
MmeI TCCRAC 1 cut(s) 1119
MnlI CCTC 9 cut(s) 22, 154, 221, 310, 345, 1237, 1464, 1672, 2035
Mox20I TGGCCA 1 cut(s) 794
Mph1103I ATGCAT 1 cut(s) 717
MroXI GAANNNNTTC 3 cut(s) 407, 1391, 1785
MscI TGGCCA 1 cut(s) 794
MseI TTAA 7 cut(s) 312, 420, 669, 735, 1023, 1476, 1625
MslI CAYNNNNRTG 1 cut(s) 23
Msp20I TGGCCA 1 cut(s) 794
MspCI CTTAAG 3 cut(s) 734, 1022, 1475
MspI CCGG 5 cut(s) 655, 974, 1574, 2000, 2012
MspR9I CCNGG 2 cut(s) 791, 1574
MunI CAATTG 2 cut(s) 573, 1955
MvaI CCWGG 1 cut(s) 791
MvnI CGCG 2 cut(s) 375, 377
MwoI GCNNNNNNNGC 2 cut(s) 542, 2054
NciI CCSGG 1 cut(s) 1574
NcoI CCATGG 2 cut(s) 557, 752
NdeII GATC 7 cut(s) 15, 40, 283, 306, 378, 459, 1612
NheI GCTAGC 1 cut(s) 2057
NlaIV GGNNCC 6 cut(s) 501, 750, 857, 1464, 1605, 1702
NmuCI GTSAC 4 cut(s) 68, 104, 982, 1547
NsiI ATGCAT 1 cut(s) 717
NspI RCATGY 2 cut(s) 867, 1621
PaeI GCATGC 1 cut(s) 1621
PauI GCGCGC 1 cut(s) 373
PciSI GCTCTTC 1 cut(s) 369
PcsI WCGNNNNNNNCGW 1 cut(s) 706
PdmI GAANNNNTTC 3 cut(s) 407, 1391, 1785
PfeI GAWTC 6 cut(s) 1016, 1156, 1833, 1901, 2003, 2015
PflMI CCANNNNNTGG 1 cut(s) 125
PkrI GCNGC 6 cut(s) 112, 361, 1767, 1924, 1948, 1965
PleI GAGTC 3 cut(s) 65, 1544, 1766
PpsI GAGTC 3 cut(s) 65, 1544, 1766
PpuMI RGGWCCY 1 cut(s) 1990
PshBI ATTAAT 2 cut(s) 312, 669
Psp124BI GAGCTC 1 cut(s) 1438
Psp1406I AACGTT 1 cut(s) 1785
Psp5II RGGWCCY 1 cut(s) 1990
Psp6I CCWGG 1 cut(s) 789
PspFI CCCAGC 2 cut(s) 237, 805
PspGI CCWGG 1 cut(s) 789
PspN4I GGNNCC 6 cut(s) 501, 750, 857, 1464, 1605, 1702
PspPI GGNCC 3 cut(s) 652, 749, 1990
PspPPI RGGWCCY 1 cut(s) 1990
PsrI GAACNNNNNNTAC 4 cut(s) 168, 200, 2016, 2048
PsuI RGATCY 1 cut(s) 40
PteI GCGCGC 1 cut(s) 373
RsaI GTAC 7 cut(s) 169, 232, 441, 785, 880, 1129, 1571
RsaNI GTAC 7 cut(s) 168, 231, 440, 784, 879, 1128, 1570
RseI CAYNNNNRTG 1 cut(s) 23
SacI GAGCTC 1 cut(s) 1438
SapI GCTCTTC 1 cut(s) 369
SaqAI TTAA 7 cut(s) 312, 420, 669, 735, 1023, 1476, 1625
SatI GCNGC 6 cut(s) 111, 360, 1766, 1923, 1947, 1964
Sau3AI GATC 7 cut(s) 15, 40, 283, 306, 378, 459, 1612
Sau96I GGNCC 3 cut(s) 652, 749, 1990
SchI GAGTC 3 cut(s) 65, 1544, 1766
ScrFI CCNGG 2 cut(s) 791, 1574
SduI GDGCHC 1 cut(s) 1438
SfaNI GCATC 5 cut(s) 508, 543, 724, 1363, 1763
SfcI CTRYAG 1 cut(s) 1863
SinI GGWCC 2 cut(s) 749, 1990
SmiMI CAYNNNNRTG 1 cut(s) 23
SmlI CTYRAG 4 cut(s) 734, 1022, 1468, 1475
SmoI CTYRAG 4 cut(s) 734, 1022, 1468, 1475
SphI GCATGC 1 cut(s) 1621
SsiI CCGC 3 cut(s) 587, 1594, 1966
SspI AATATT 2 cut(s) 692, 1008
SspMI CTAG 8 cut(s) 447, 611, 947, 1640, 1665, 1761, 2021, 2058
SstI GAGCTC 1 cut(s) 1438
StyD4I CCNGG 2 cut(s) 789, 1572
StyI CCWWGG 4 cut(s) 331, 557, 752, 1848
TaaI ACNGT 2 cut(s) 444, 993
TaiI ACGT 3 cut(s) 169, 1392, 1788
TaqI TCGA 6 cut(s) 226, 432, 458, 547, 1268, 1770
TatI WGTACW 3 cut(s) 439, 783, 878
TfiI GAWTC 6 cut(s) 1016, 1156, 1833, 1901, 2003, 2015
Tru1I TTAA 7 cut(s) 312, 420, 669, 735, 1023, 1476, 1625
Tru9I TTAA 7 cut(s) 312, 420, 669, 735, 1023, 1476, 1625
TscAI CASTG 3 cut(s) 388, 1569, 1903
TseFI GTSAC 4 cut(s) 68, 104, 982, 1547
TseI GCWGC 6 cut(s) 110, 359, 1765, 1922, 1946, 1963
Tsp45I GTSAC 4 cut(s) 68, 104, 982, 1547
TspDTI ATGAA 9 cut(s) 17, 189, 385, 743, 884, 921, 925, 966, 1752
TspGWI ACGGA 2 cut(s) 715, 1746
TspRI CASTG 3 cut(s) 388, 1569, 1903
Van91I CCANNNNNTGG 1 cut(s) 125
Vha464I CTTAAG 3 cut(s) 734, 1022, 1475
VpaK11BI GGWCC 2 cut(s) 749, 1990
VspI ATTAAT 2 cut(s) 312, 669
XapI RAATTY 7 cut(s) 139, 346, 525, 953, 1048, 1419, 1880
XbaI TCTAGA 2 cut(s) 446, 946
XceI RCATGY 2 cut(s) 867, 1621
XcmI CCANNNNNNNNNTGG 1 cut(s) 802
XmnI GAANNNNTTC 3 cut(s) 407, 1391, 1785
XspI CTAG 8 cut(s) 447, 611, 947, 1640, 1665, 1761, 2021, 2058
Zsp2I ATGCAT 1 cut(s) 717
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.