Rw3G024400

Cyclic nucleotide-gated ion channel 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0000119
Physical Location & Seq
Reverse (-)
31321694 .. 31325122
3429 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G024400.1

Sequence Viewer

Length: 1836 bp
ATGTCGGTACCCGATGATGTCCCTATGATGAGCATCACACGGGGAATTAAGTACAATTTCGACAATGTTGAGGCGCAACCGCCAGATCCCCCGTGGAGAGATCGAGATGCAGTTTCAGCAAAACAAAAATTGGAAGGAACACTTCTTTCAGTTTCATTCTGGAATAAGATGTTTGTAATTGCATGTGTGATTGCAGTCTCATTAGACCCATTGTTCTTCTACATTCCAATCATCGATGAAGAAGAAAAGTGCCTTGCAGAAGACAAAGAGTTGAGGACTATAGCTCTCATTTTGCGATCGCTTACTGATGTCACTTTCGTAGTGAATATCATATATCATGTTTGCAAAGCCATTAACGCTGCCTACAAAGAACACAAAGGAAAAAAGTCTGAATTGGCATCGGATTGGGAATTTTCGAAGGTATCAGCAGCGGAGATAATTCCATTTGCTAAATTAGTTGCTGGGAAGCTAGCATGGCGCTCTACGCTAACCAGCATTTTAGCTGTTTTTCCCATGCCACAACTGCTTCTAGTATTTGTCTTCTTCAAAATGAGAGGCCACGCATACTTGCAGCGTAGAAAGATCCTAAATGTCTTTCTTCTCGCTCAATATTTGCCAAGGATCTATCGAATTTATCTATCATCTCAGAACCTCAGACAAACTACTGGATTATGGGCTAAAGCTCTGTTTAACTTTTTTCTATATATTCTTGCAAGTCATGTCCTTGGAGCTTTTTGGTACTTTTTTTCCATTCAACGAGAAACTTCTTGTTGGCATCAGGCATGTGTAAATAATAGTACGAATGTTGAAGGATGTATGAGTACCTTATACTGCGAGGTTCACAATACTGATGCAAGAAATGCCACATTCCTTAATCAATTTTGCCCTATAAGTGCTGAAGAGAATGCTAAGGCCCCTTTTGATTTTGGAATATTTCTGGATTCCCTCAAAAATGGTATCACAGGAGATATACATTTCAGAACGAAGTTATCTTACTCTTTTTGGTGGGGATTACGTAATCTAAGTAATTTTGGCACAAATCTCGAAACAAGCACCTATGTGTGGGAAACTTGCTTTGCAGTTCTTATTTCTGTGATTGGCTTGCTACTATTTCTATATCTCATTGGAAATGTACAGACATTTATGTCCATGGAAACTCAAAGATGGGAGGATATAAGAAACAAGATACAATTGAAAGAGAAGGATATAGCAGGATGGGTGGACAGAAATGAGCTCCCAGACAATTTGAAGAAAGAAATTATGAAGAACATAGAGCAAAAACTGGAAGAGAACAAAGATACTGATCTTGAGAATCTTTTCTCTATTCTTCCGACGTATACCAAAATGTGTCTAAAGCGTTTCCTCGGCATGAAATTGCTAAAGAAAGTACCGAAGCTTGCAAGGATGGATGAAAGAGTGTTGAAAATGATGTGCGACTATCTAAAGCCAGTGATGTTTGGCGAGGGCACCGAGGTTTTACAAATGGGAGTTCCACTAAATAGCATGCTACTAATCATAGAAGGCACATTATTGATCTACAGAACTACCAGTGATGATTGCCATGTTGCGACTGAAAACGCAAAAACGGGTAGTATTTCAAGTTCCCCGTCAGTGGGCAACCTTGACAAAGGTGACTTTTACGGCGCTGAAGAACTGATCGAGTGGGTAACACAAAAGAAGGATTTAGGCCAGCTTCCCGGCTCTTCTTTCAATGTGAAATGCGATTCGAAAGTAGAAGGCTTTGTTCTCACGGCCAAGGACTTGGAAAATGTAGTTTCCAAAAACGAAAACTGGTGGAAAATTGGCATTAGCAAGTCAGACCACAACATATTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
Ion_trans

Protein Analysis

611

Amino Acids

69.92

Weight (kDa)

7.08

Isoelectric Point (pI)

40.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ion_trans PF00520 53 - 389 3.7e-07 Ion transport protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000119)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g10570 FvH4_4g36390 FvH4_7g31840 FvH4_7g31840 FvH4_7g31850 FvH4_7g31850 FvH4_7g31861 FvH4_7g31861 FvH4_7g31950
malus_domestica MD01G1054600.v1.1 MD01G1221100.v1.1 MD01G1221400.v1.1 MD01G1221600.v1.1 MD01G1221800.v1.1 MD01G1222000.v1.1 MD01G1222100.v1.1 MD07G1140800.v1.1 MD07G1291200.v1.1 MD07G1291400.v1.1 MD07G1291500.v1.1 MD07G1291700.v1.1 MD07G1292500.v1.1 MD07G1292700.v1.1 MD07G1292800.v1.1 MD07G1292900.v1.1 MD08G1241600.v1.1 MD11G1083800.v1.1 MD15G1431600.v1.1
prunus_persica Prupe.1G017800_v2.0.a1 Prupe.1G017800_v2.0.a1 Prupe.1G162800_v2.0.a1 Prupe.2G312500_v2.0.a1 Prupe.2G312500_v2.0.a1 Prupe.2G312700_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092800_v2.0.a1 Prupe.4G092800_v2.0.a1 Prupe.6G142800_v2.0.a1 Prupe.6G142800_v2.0.a1 Prupe.6G143000_v2.0.a1 Prupe.6G143100_v2.0.a1 Prupe.6G143100_v2.0.a1 Prupe.6G143100_v2.0.a1 Prupe.6G143100_v2.0.a1
pyrus_communis pycom01g23050 pycom01g23070 pycom01g23090 pycom01g23100 pycom01g23110 pycom01g23120 pycom01g23170 pycom07g26410 pycom07g26440 pycom07g26470 pycom07g26480 pycom07g26490 pycom07g26500 pycom07g26510 pycom07g26530 pycom07g26540 pycom07g26570 pycom07g26580 pycom07g26590 pycom08g20940
rosa_chinensis RchiOBHm_Chr1g0324571 RchiOBHm_Chr1g0336181 RchiOBHm_Chr1g0381061 RchiOBHm_Chr1g0381091 RchiOBHm_Chr1g0381121 RchiOBHm_Chr1g0381131 RchiOBHm_Chr1g0381141 RchiOBHm_Chr1g0381161 RchiOBHm_Chr1g0381211 RchiOBHm_Chr2g0110361 RchiOBHm_Chr2g0120231 RchiOBHm_Chr3g0486281 RchiOBHm_Chr3g0486311 RchiOBHm_Chr3g0486401 RchiOBHm_Chr4g0409511 RchiOBHm_Chr4g0409521 RchiOBHm_Chr5g0017271 RchiOBHm_Chr5g0048781 RchiOBHm_Chr5g0048811
rosa_laevigata RLG00000002401 RLG00000008559 RLG00000017798 RLG00000017799 RLG00000023082 RLG00000023088 RLG00000026230 RLG00000026238 RLG00000026240 RLG00000026241 RLG00000026242 RLG00000026243 RLG00000026244 RLG00000026246 RLG00000026247 RLG00000026248 RLG00000029876 RLG00000030189 RLG00000034579
rosa_multiflora Rmu_co8101374.1_g000001 Rmu_co8309149.1_g000001 Rmu_co8450063.1_g000001 Rmu_sc0000441.1_g000094 Rmu_sc0000479.1_g000005 Rmu_sc0000647.1_g000006 Rmu_sc0001393.1_g000031 Rmu_sc0001423.1_g000037 Rmu_sc0001616.1_g000015 Rmu_sc0001857.1_g000011 Rmu_sc0002183.1_g000013 Rmu_sc0002183.1_g000028 Rmu_sc0002183.1_g000030 Rmu_sc0002688.1_g000003 Rmu_sc0002688.1_g000020 Rmu_sc0003801.1_g000003 Rmu_sc0003801.1_g000004 Rmu_sc0004125.1_g000006 Rmu_sc0004494.1_g000005 Rmu_sc0004494.1_g000006 Rmu_sc0004494.1_g000008 Rmu_sc0004494.1_g000009 Rmu_sc0005842.1_g000004 Rmu_sc0006872.1_g000001 Rmu_sc0006872.1_g000005 Rmu_sc0006872.1_g000009 Rmu_sc0006872.1_g000010 Rmu_sc0008053.1_g000026
rosa_roxburghii Rroxscaffold_159G00432960 Rroxscaffold_1G00033060 Rroxscaffold_2G00123150 Rroxscaffold_2G00123170 Rroxscaffold_2G00133500 Rroxscaffold_2G00133550 Rroxscaffold_4G00278650 Rroxscaffold_4G00278750 Rroxscaffold_4G00278790 Rroxscaffold_4G00278800 Rroxscaffold_4G00278810 Rroxscaffold_4G00325390 Rroxscaffold_5G00354010 Rroxscaffold_6G00395890 Rroxscaffold_6G00395900 Rroxscaffold_6G00395920
rosa_rugosa Rorug01G0046900.1 Rorug01G0427000 Rorug01G0427100 Rorug01G0427200 Rorug01G0427300 Rorug01G0427400 Rorug01G0427500 Rorug01G0427500 Rorug01G0427600 Rorug01G0427600 Rorug01G0427700 Rorug01G0427700 Rorug01G0427800 Rorug01G0428700 Rorug02G0166400 Rorug02G0166500 Rorug02G0166500 Rorug02G0166600 Rorug02G0166600 Rorug02G0166700 Rorug02G0167300 Rorug02G0167300 Rorug02G0167300 Rorug03G0224600 Rorug03G0224600 Rorug04G0094400 Rorug05G0241100 Rorug05G0241200
rosa_samantha Rh1BG407700 Rh1BG407900 Rh1BG409000 Rh1CG064700 Rh1CG420900 Rh1CG421000 Rh1CG421200 Rh1CG421300 Rh1CG421800 Rh1CG421900 Rh1CG422000 Rh1CG422200 Rh1CG422300 Rh1CG423600 Rh1DG437600 Rh1DG438900 Rh1DG439000 Rh1DG440300 Rh2AG219100 Rh2CG220700 Rh2DG225300 Rh3AG274400 Rh3AG274600 Rh3CG308900 Rh3DG305800 Rh4AG156400 Rh5AG129000 Rh5AG320900 Rh5CG139600
rosa_wichuraiana Rw1G005300 Rw1G039280 Rw1G039300 Rw1G039310 Rw1G039320 Rw1G039330 Rw1G039340 Rw1G039360 Rw1G039390 Rw2G016910 Rw2G022800 Rw3G024330 Rw3G024340 Rw3G024350 Rw3G024400 Rw4G012810 Rw5G030120 Rw5G030280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1144
Acc65I GGTACC 1 cut(s) 7
AccB1I GGYRCC 2 cut(s) 7, 1466
AccI GTMKAC 1 cut(s) 1337
AciI CCGC 2 cut(s) 80, 431
AclWI GGATC 3 cut(s) 80, 577, 629
AcoI YGGCCR 1 cut(s) 1752
AcsI RAATTY 2 cut(s) 410, 630
AcuI CTGAAG 2 cut(s) 918, 1668
AfaI GTAC 7 cut(s) 9, 53, 740, 799, 823, 1134, 1389
AfiI CCNNNNNNNGG 3 cut(s) 1062, 1612, 1613
AgsI TTSAA 8 cut(s) 547, 755, 809, 1195, 1249, 1423, 1599, 1711
AleI CACNNNNGTG 1 cut(s) 1058
AluBI AGCT 8 cut(s) 284, 469, 503, 683, 731, 1234, 1396, 1693
AluI AGCT 8 cut(s) 284, 469, 503, 683, 731, 1234, 1396, 1693
Alw21I GWGCWC 1 cut(s) 1236
Alw26I GTCTC 1 cut(s) 202
AlwI GGATC 3 cut(s) 80, 577, 629
AoxI GGCC 4 cut(s) 556, 912, 1687, 1752
ApeKI GCWGC 3 cut(s) 359, 428, 571
ApoI RAATTY 2 cut(s) 410, 630
AsiSI GCGATCGC 1 cut(s) 299
Asp700I GAANNNNTTC 1 cut(s) 1316
Asp718I GGTACC 1 cut(s) 7
AspLEI GCGC 3 cut(s) 76, 480, 1646
AspS9I GGNCC 1 cut(s) 913
AsuC2I CCSGG 1 cut(s) 1698
AsuHPI GGTGA 1 cut(s) 1643
AsuII TTCGAA 2 cut(s) 416, 1727
AsuNHI GCTAGC 1 cut(s) 469
BaeGI GKGCMC 1 cut(s) 1469
BanI GGYRCC 2 cut(s) 7, 1466
BanII GRGCYC 1 cut(s) 1236
BarI GAAGNNNNNNTAC 2 cut(s) 977, 1009
BbsI GAAGAC 2 cut(s) 267, 532
Bbv12I GWGCWC 1 cut(s) 1236
BbvI GCAGC 3 cut(s) 346, 440, 583
BccI CCATC 3 cut(s) 1158, 1209, 1399
BceAI ACGGC 2 cut(s) 1657, 1767
BcgI CGANNNNNNTGC 2 cut(s) 1024, 1058
BcnI CCSGG 1 cut(s) 1698
BcoDI GTCTC 1 cut(s) 202
BfaI CTAG 3 cut(s) 470, 530, 1834
BfmI CTRYAG 2 cut(s) 279, 1537
BfoI RGCGCY 2 cut(s) 481, 1647
BisI GCNGC 3 cut(s) 360, 429, 572
BlsI GCNGC 3 cut(s) 361, 430, 573
Bme1390I CCNGG 1 cut(s) 1698
BmgT120I GGNCC 1 cut(s) 913
BmiI GGNNCC 3 cut(s) 9, 915, 1468
BmrFI CCNGG 1 cut(s) 1698
BmsI GCATC 5 cut(s) 42, 97, 407, 784, 841
BmtI GCTAGC 1 cut(s) 473
BpiI GAAGAC 2 cut(s) 267, 532
Bpu10I CCTNAGC 1 cut(s) 909
Bpu14I TTCGAA 2 cut(s) 416, 1727
BpuEI CTTGAG 1 cut(s) 1328
BpuMI CCSGG 1 cut(s) 1698
Bsa29I ATCGAT 1 cut(s) 234
BsaAI YACGTR 1 cut(s) 1016
BsaBI GATNNNNATC 2 cut(s) 32, 1302
BsaJI CCNNGG 7 cut(s) 92, 617, 724, 1149, 1363, 1470, 1755
Bsc4I CCNNNNNNNGG 3 cut(s) 1062, 1612, 1613
Bse1I ACTGG 5 cut(s) 670, 1287, 1448, 1548, 1796
Bse8I GATNNNNATC 2 cut(s) 32, 1302
BseCI ATCGAT 1 cut(s) 234
BseDI CCNNGG 7 cut(s) 92, 617, 724, 1149, 1363, 1470, 1755
BseGI GGATG 4 cut(s) 818, 1220, 1410, 1414
BseJI GATNNNNATC 2 cut(s) 32, 1302
BseLI CCNNNNNNNGG 3 cut(s) 1062, 1612, 1613
BseMII CTCAG 2 cut(s) 659, 667
BseNI ACTGG 5 cut(s) 670, 1287, 1448, 1548, 1796
BseSI GKGCMC 1 cut(s) 1469
BseXI GCAGC 3 cut(s) 346, 440, 583
BseYI CCCAGC 1 cut(s) 461
Bsh1285I CGRYCG 1 cut(s) 299
BshFI GGCC 4 cut(s) 558, 914, 1689, 1754
BshNI GGYRCC 2 cut(s) 7, 1466
BshVI ATCGAT 1 cut(s) 234
BsiEI CGRYCG 1 cut(s) 299
BsiHKAI GWGCWC 1 cut(s) 1236
BsiSI CCGG 1 cut(s) 1698
BslFI GGGAC 1 cut(s) 5
BslI CCNNNNNNNGG 3 cut(s) 1062, 1612, 1613
BsmAI GTCTC 1 cut(s) 202
BsmFI GGGAC 1 cut(s) 5
BsmI GAATGC 1 cut(s) 910
BsnI GGCC 4 cut(s) 558, 914, 1689, 1754
Bsp119I TTCGAA 2 cut(s) 416, 1727
Bsp1286I GDGCHC 2 cut(s) 1236, 1469
Bsp1407I TGTACA 1 cut(s) 1132
Bsp143I GATC 8 cut(s) 85, 100, 296, 582, 621, 1303, 1533, 1656
Bsp19I CCATGG 1 cut(s) 1149
BspACI CCGC 2 cut(s) 80, 431
BspANI GGCC 4 cut(s) 558, 914, 1689, 1754
BspCNI CTCAG 2 cut(s) 658, 666
BspDI ATCGAT 1 cut(s) 234
BspLI GGNNCC 3 cut(s) 9, 915, 1468
BspOI GCTAGC 1 cut(s) 473
BspPI GGATC 3 cut(s) 80, 577, 629
BspQI GCTCTTC 1 cut(s) 1708
BspT104I TTCGAA 2 cut(s) 416, 1727
BspT107I GGYRCC 2 cut(s) 7, 1466
BsrGI TGTACA 1 cut(s) 1132
BsrI ACTGG 5 cut(s) 670, 1287, 1448, 1548, 1796
BssECI CCNNGG 7 cut(s) 92, 617, 724, 1149, 1363, 1470, 1755
BssMI GATC 8 cut(s) 85, 100, 296, 582, 621, 1303, 1533, 1656
BssNAI GTATAC 1 cut(s) 1338
BssT1I CCWWGG 4 cut(s) 617, 724, 1149, 1755
Bst1107I GTATAC 1 cut(s) 1338
Bst6I CTCTTC 3 cut(s) 894, 1281, 1708
BstAPI GCANNNNNTGC 1 cut(s) 860
BstAUI TGTACA 1 cut(s) 1132
BstBAI YACGTR 1 cut(s) 1016
BstBI TTCGAA 2 cut(s) 416, 1727
BstC8I GCNNGC 5 cut(s) 471, 1103, 1398, 1505, 1691
BstDEI CTNAG 4 cut(s) 645, 653, 909, 1022
BstDSI CCRYGG 2 cut(s) 92, 1149
BstF5I GGATG 4 cut(s) 818, 1220, 1410, 1414
BstH2I RGCGCY 2 cut(s) 481, 1647
BstHHI GCGC 3 cut(s) 76, 480, 1646
BstKTI GATC 8 cut(s) 88, 103, 299, 585, 624, 1306, 1536, 1659
BstMAI GTCTC 1 cut(s) 202
BstMBI GATC 8 cut(s) 85, 100, 296, 582, 621, 1303, 1533, 1656
BstMCI CGRYCG 1 cut(s) 299
BstMWI GCNNNNNNNGC 6 cut(s) 116, 356, 475, 484, 523, 860
BstNSI RCATGY 3 cut(s) 186, 786, 1507
BstSCI CCNGG 1 cut(s) 1696
BstSFI CTRYAG 2 cut(s) 279, 1537
BstSLI GKGCMC 1 cut(s) 1469
BstSNI TACGTA 1 cut(s) 1016
BstV1I GCAGC 3 cut(s) 346, 440, 583
BstV2I GAAGAC 2 cut(s) 267, 532
BstX2I RGATCY 3 cut(s) 85, 582, 621
BstXI CCANNNNNNTGG 1 cut(s) 1762
BstYI RGATCY 3 cut(s) 85, 582, 621
BstZ17I GTATAC 1 cut(s) 1338
Bsu15I ATCGAT 1 cut(s) 234
BsuRI GGCC 4 cut(s) 558, 914, 1689, 1754
BsuTUI ATCGAT 1 cut(s) 234
BtgI CCRYGG 2 cut(s) 92, 1149
BtsCI GGATG 4 cut(s) 818, 1220, 1410, 1414
BtsIMutI CAGTG 3 cut(s) 1455, 1555, 1617
Cac8I GCNNGC 5 cut(s) 471, 1103, 1398, 1505, 1691
CfoI GCGC 3 cut(s) 76, 480, 1646
Cfr13I GGNCC 1 cut(s) 913
ClaI ATCGAT 1 cut(s) 234
Csp6I GTAC 7 cut(s) 8, 52, 739, 798, 822, 1133, 1388
CviQI GTAC 7 cut(s) 8, 52, 739, 798, 822, 1133, 1388
DdeI CTNAG 4 cut(s) 645, 653, 909, 1022
DpnI GATC 8 cut(s) 87, 102, 298, 584, 623, 1305, 1535, 1658
DpnII GATC 8 cut(s) 85, 100, 296, 582, 621, 1303, 1533, 1656
DrdI GACNNNNNNGTC 1 cut(s) 1144
DseDI GACNNNNNNGTC 1 cut(s) 1144
EaeI YGGCCR 1 cut(s) 1752
Eam1104I CTCTTC 3 cut(s) 894, 1281, 1708
EarI CTCTTC 3 cut(s) 894, 1281, 1708
Ecl136II GAGCTC 1 cut(s) 1234
Eco105I TACGTA 1 cut(s) 1016
Eco130I CCWWGG 4 cut(s) 617, 724, 1149, 1755
Eco24I GRGCYC 1 cut(s) 1236
Eco53kI GAGCTC 1 cut(s) 1234
Eco57I CTGAAG 2 cut(s) 918, 1668
EcoICRI GAGCTC 1 cut(s) 1234
EcoO109I RGGNCCY 1 cut(s) 913
EcoT14I CCWWGG 4 cut(s) 617, 724, 1149, 1755
EcoT38I GRGCYC 1 cut(s) 1236
ErhI CCWWGG 4 cut(s) 617, 724, 1149, 1755
FalI AAGNNNNNCTT 2 cut(s) 126, 158
FaqI GGGAC 1 cut(s) 5
FblI GTMKAC 1 cut(s) 1337
Fnu4HI GCNGC 3 cut(s) 360, 429, 572
FokI GGATG 4 cut(s) 825, 1227, 1417, 1421
FriOI GRGCYC 1 cut(s) 1236
Fsp4HI GCNGC 3 cut(s) 360, 429, 572
FspBI CTAG 3 cut(s) 470, 530, 1834
GlaI GCGC 3 cut(s) 75, 479, 1645
GluI GCNGC 3 cut(s) 360, 429, 572
GsaI CCCAGC 1 cut(s) 465
HaeII RGCGCY 2 cut(s) 481, 1647
HaeIII GGCC 4 cut(s) 558, 914, 1689, 1754
HapII CCGG 1 cut(s) 1698
HhaI GCGC 3 cut(s) 76, 480, 1646
Hin6I GCGC 3 cut(s) 74, 478, 1644
HinP1I GCGC 3 cut(s) 74, 478, 1644
HindIII AAGCTT 1 cut(s) 1394
HinfI GANTC 3 cut(s) 941, 1312, 1724
HpaII CCGG 1 cut(s) 1698
HphI GGTGA 1 cut(s) 1643
Hpy166II GTNNAC 3 cut(s) 841, 1222, 1338
Hpy188I TCNGA 7 cut(s) 391, 403, 648, 656, 980, 1332, 1819
Hpy188III TCNNGA 5 cut(s) 104, 160, 938, 1043, 1307
Hpy8I GTNNAC 3 cut(s) 841, 1222, 1338
Hpy99I CGWCG 1 cut(s) 1336
HpyAV CCTTC 7 cut(s) 128, 412, 803, 1195, 1514, 1672, 1730
HpyCH4IV ACGT 2 cut(s) 1015, 1334
HpyF10VI GCNNNNNNNGC 6 cut(s) 116, 356, 475, 484, 523, 860
HpyF3I CTNAG 4 cut(s) 645, 653, 909, 1022
HpySE526I ACGT 2 cut(s) 1015, 1334
HspAI GCGC 3 cut(s) 74, 478, 1644
KpnI GGTACC 1 cut(s) 11
Kzo9I GATC 8 cut(s) 85, 100, 296, 582, 621, 1303, 1533, 1656
LguI GCTCTTC 1 cut(s) 1708
LmnI GCTCC 2 cut(s) 728, 1239
Lsp1109I GCAGC 3 cut(s) 346, 440, 583
LweI GCATC 5 cut(s) 42, 97, 407, 784, 841
MaeI CTAG 3 cut(s) 470, 530, 1834
MaeII ACGT 2 cut(s) 1015, 1334
MaeIII GTNAC 3 cut(s) 310, 1631, 1666
MalI GATC 8 cut(s) 87, 102, 298, 584, 623, 1305, 1535, 1658
MboI GATC 8 cut(s) 85, 100, 296, 582, 621, 1303, 1533, 1656
MfeI CAATTG 1 cut(s) 1190
MflI RGATCY 3 cut(s) 85, 582, 621
MhlI GDGCHC 2 cut(s) 1236, 1469
MmeI TCCRAC 1 cut(s) 1355
MroXI GAANNNNTTC 1 cut(s) 1316
MseI TTAA 4 cut(s) 48, 354, 690, 873
MslI CAYNNNNRTG 1 cut(s) 1058
MspA1I CMGCKG 1 cut(s) 431
MspI CCGG 1 cut(s) 1698
MspR9I CCNGG 1 cut(s) 1698
MunI CAATTG 1 cut(s) 1190
Mva1269I GAATGC 1 cut(s) 910
MwoI GCNNNNNNNGC 6 cut(s) 116, 356, 475, 484, 523, 860
NciI CCSGG 1 cut(s) 1698
NcoI CCATGG 1 cut(s) 1149
NdeII GATC 8 cut(s) 85, 100, 296, 582, 621, 1303, 1533, 1656
NheI GCTAGC 1 cut(s) 469
NlaIV GGNNCC 3 cut(s) 9, 915, 1468
NmeAIII GCCGAG 1 cut(s) 1344
NmuCI GTSAC 2 cut(s) 310, 1631
NspI RCATGY 3 cut(s) 186, 786, 1507
NspV TTCGAA 2 cut(s) 416, 1727
OliI CACNNNNGTG 1 cut(s) 1058
PaeI GCATGC 1 cut(s) 1507
PciSI GCTCTTC 1 cut(s) 1708
PctI GAATGC 1 cut(s) 910
PdmI GAANNNNTTC 1 cut(s) 1316
PfeI GAWTC 3 cut(s) 941, 1312, 1724
PkrI GCNGC 3 cut(s) 361, 430, 573
Ple19I CGATCG 1 cut(s) 299
Ppu21I YACGTR 1 cut(s) 1016
Psp124BI GAGCTC 1 cut(s) 1236
PspFI CCCAGC 1 cut(s) 461
PspN4I GGNNCC 3 cut(s) 9, 915, 1468
PspPI GGNCC 1 cut(s) 913
PsrI GAACNNNNNNTAC 2 cut(s) 1585, 1617
PsuI RGATCY 3 cut(s) 85, 582, 621
PvuI CGATCG 1 cut(s) 299
RgaI GCGATCGC 1 cut(s) 299
RsaI GTAC 7 cut(s) 9, 53, 740, 799, 823, 1134, 1389
RsaNI GTAC 7 cut(s) 8, 52, 739, 798, 822, 1133, 1388
RseI CAYNNNNRTG 1 cut(s) 1058
SacI GAGCTC 1 cut(s) 1236
SapI GCTCTTC 1 cut(s) 1708
SaqAI TTAA 4 cut(s) 48, 354, 690, 873
SatI GCNGC 3 cut(s) 360, 429, 572
Sau3AI GATC 8 cut(s) 85, 100, 296, 582, 621, 1303, 1533, 1656
Sau96I GGNCC 1 cut(s) 913
ScrFI CCNGG 1 cut(s) 1698
SduI GDGCHC 2 cut(s) 1236, 1469
SfaAI GCGATCGC 1 cut(s) 299
SfaNI GCATC 5 cut(s) 42, 97, 407, 784, 841
SfcI CTRYAG 2 cut(s) 279, 1537
SfuI TTCGAA 2 cut(s) 416, 1727
SgfI GCGATCGC 1 cut(s) 299
SmiMI CAYNNNNRTG 1 cut(s) 1058
SmlI CTYRAG 1 cut(s) 1307
SmoI CTYRAG 1 cut(s) 1307
SnaBI TACGTA 1 cut(s) 1016
SphI GCATGC 1 cut(s) 1507
SsiI CCGC 2 cut(s) 80, 431
SspI AATATT 2 cut(s) 611, 933
SspMI CTAG 3 cut(s) 470, 530, 1834
SstI GAGCTC 1 cut(s) 1236
StyD4I CCNGG 1 cut(s) 1696
StyI CCWWGG 4 cut(s) 617, 724, 1149, 1755
TaiI ACGT 2 cut(s) 1018, 1337
TaqI TCGA 8 cut(s) 60, 103, 234, 416, 628, 1044, 1659, 1727
TatI WGTACW 2 cut(s) 51, 1132
TfiI GAWTC 3 cut(s) 941, 1312, 1724
Tru1I TTAA 4 cut(s) 48, 354, 690, 873
Tru9I TTAA 4 cut(s) 48, 354, 690, 873
TscAI CASTG 3 cut(s) 1455, 1555, 1617
TseFI GTSAC 2 cut(s) 310, 1631
TseI GCWGC 3 cut(s) 359, 428, 571
Tsp45I GTSAC 2 cut(s) 310, 1631
TspDTI ATGAA 5 cut(s) 144, 252, 1277, 1385, 1425
TspRI CASTG 3 cut(s) 1455, 1555, 1617
XapI RAATTY 2 cut(s) 410, 630
XceI RCATGY 3 cut(s) 186, 786, 1507
XcmI CCANNNNNNNNNTGG 1 cut(s) 90
XmiI GTMKAC 1 cut(s) 1337
XmnI GAANNNNTTC 1 cut(s) 1316
XspI CTAG 3 cut(s) 470, 530, 1834
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.