Rw5G030120

Cyclic nucleotide-gated ion channel 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000119
Physical Location & Seq
Forward (+)
47472322 .. 47476079
3758 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G030120.1

Sequence Viewer

Length: 1770 bp
ATGATGAGTAATTTGGAGAAAACCCGGAAGAGCCCATATATAACTCGAGTCATAGTGGATCCATTGTTCTTTTACCTTCCAATAATTGATGGGAAAAGAAAGTGCATTGGAATGGACACAATATTGAGGAATGTGGCACTTATTTTCCGATCACTCACCGATATCGGTTTCATAGTGAATATTATACATAAAATTCGTGAAGCTGTGAATAAAACAAGAGCAGATTGTCTTAAAAACATTGAAAAAATGATTGCTGAAGAACGAAATCGAGGTGAGAGCGGAAATAAGTTTGCTAAGTCATTAGCTCGTAACTTGTCGTGGCAGACCATCTTAATCGACTTTTTGGCTGTTCTTCCCATCCCCCAAGTGATAATAGTAGCTGTTAGAAGCTCTAGATGTTTGGATAAAAGAAAGATTCTAAACTTTCTTCTTCTCGCCCAGTATCTGCCGAGGGTTTATCGAATGCGTCATTCATCTATACAACTTAGATCAAGAACTGGAATATGGGTTAAAGGTTCATTCTATTTTTTCTTGTACATCCTTGCCAGCCATGTACTTGGAGCCTTTTGGTATTATTTTTCTATTCAACAAGAGACATCTTGTTGGCATCGGGCTTGCAAAAAGCATGGTGGAGTAAAATGTAATTTTTACTGCCATGAAAACATGACTCCAAGAAATTTGACATTCCCCATCAATTCTATAGATAAATATTGCCTTGTAGATGTTCCGGCAAATGAAACTGCACCATTTGATTTTGGAATCTTTCTTGATTCTCTTAAGAATAAGAACACGGCATCAATACAATTTCCAAAGAAGTTTTTCTACTCTTTTTGGTGGGGACTTCGAAATCTGAGTAATTTTGGCACAAATTTGCAAACAAGTACTTATGTGTGGGAAAACTGCTTTGCAATTCTGATTTCAATCATTGGCCTGCTATTATTCTTATATCTCATTGGAAAAGTACAGACATTTATTTCGATGAAAACTGCGAATTCCGAGGAGAGAAGAAAAAAATTCAAATCAAAAGAGCTAGATATACAAATGTGGATGAAAAGCAATGATCTCAAGAGAGATATGATGGAAGACATCAGAGTACATACAAATGAAAAGTGGGAAGAAATACGAGATGCAAATATAGAGAACCTCATCTCTATTCTTCCCGTGCAATCTCGGAAAAATCTTAAACATTCTCTTTGCATGGAAATGCTGAGCAAGGTGCCAAAGCTTAAAACTCTGAAAGGTGAAGCTTTGACGTTGATCTGCGATCGTCTGAAGCCAGTGGTCTATCAGGATAATAGCTTTGTTTTCCAAGAAGGAGAACCACTTGATCGCATTCTTTTCATTACGGAAGGCTTAATATGGACATACCAGGCGGCGGGCACTACTGATAGTAGTAGCACTTGTCAAAATGGAATAGGAAAAACAGGTTTCAGTTTTCTTGAGAAAGGTGGATTTTACGGAGACGATCAACTTCTGAGTTGGGCTATAGCATCACACAAGAATGATCTTTCCTTTAGCAACCTGCCAATCTCCAAAGTGAATGTGAAATGCCATGCAAAAGTAGAAGGCTTTGTACTCATGGCGAAGGACTTGAAAACTGTAGTCTCCGCTAATTACATAGTTTATTGGGGTTTCCACGATTCTTCTCAGGAAAGAGAGGAGGCAGCAATTCAAGCCATAAAGAAAGTATGGGATCGTCGTAACAAAGATAGACCAAAGGGAAATCCAATGATGTCACTCCCAGCCGGGCCACTGGCTACAATATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
Ion_trans

Protein Analysis

589

Amino Acids

67.9

Weight (kDa)

9.48

Isoelectric Point (pI)

42.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000119)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g10570 FvH4_4g36390 FvH4_7g31840 FvH4_7g31840 FvH4_7g31850 FvH4_7g31850 FvH4_7g31861 FvH4_7g31861 FvH4_7g31950
malus_domestica MD01G1054600.v1.1 MD01G1221100.v1.1 MD01G1221400.v1.1 MD01G1221600.v1.1 MD01G1221800.v1.1 MD01G1222000.v1.1 MD01G1222100.v1.1 MD07G1140800.v1.1 MD07G1291200.v1.1 MD07G1291400.v1.1 MD07G1291500.v1.1 MD07G1291700.v1.1 MD07G1292500.v1.1 MD07G1292700.v1.1 MD07G1292800.v1.1 MD07G1292900.v1.1 MD08G1241600.v1.1 MD11G1083800.v1.1 MD15G1431600.v1.1
prunus_persica Prupe.1G017800_v2.0.a1 Prupe.1G017800_v2.0.a1 Prupe.1G162800_v2.0.a1 Prupe.2G312500_v2.0.a1 Prupe.2G312500_v2.0.a1 Prupe.2G312700_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092800_v2.0.a1 Prupe.4G092800_v2.0.a1 Prupe.6G142800_v2.0.a1 Prupe.6G142800_v2.0.a1 Prupe.6G143000_v2.0.a1 Prupe.6G143100_v2.0.a1 Prupe.6G143100_v2.0.a1 Prupe.6G143100_v2.0.a1 Prupe.6G143100_v2.0.a1
pyrus_communis pycom01g23050 pycom01g23070 pycom01g23090 pycom01g23100 pycom01g23110 pycom01g23120 pycom01g23170 pycom07g26410 pycom07g26440 pycom07g26470 pycom07g26480 pycom07g26490 pycom07g26500 pycom07g26510 pycom07g26530 pycom07g26540 pycom07g26570 pycom07g26580 pycom07g26590 pycom08g20940
rosa_chinensis RchiOBHm_Chr1g0324571 RchiOBHm_Chr1g0336181 RchiOBHm_Chr1g0381061 RchiOBHm_Chr1g0381091 RchiOBHm_Chr1g0381121 RchiOBHm_Chr1g0381131 RchiOBHm_Chr1g0381141 RchiOBHm_Chr1g0381161 RchiOBHm_Chr1g0381211 RchiOBHm_Chr2g0110361 RchiOBHm_Chr2g0120231 RchiOBHm_Chr3g0486281 RchiOBHm_Chr3g0486311 RchiOBHm_Chr3g0486401 RchiOBHm_Chr4g0409511 RchiOBHm_Chr4g0409521 RchiOBHm_Chr5g0017271 RchiOBHm_Chr5g0048781 RchiOBHm_Chr5g0048811
rosa_laevigata RLG00000002401 RLG00000008559 RLG00000017798 RLG00000017799 RLG00000023082 RLG00000023088 RLG00000026230 RLG00000026238 RLG00000026240 RLG00000026241 RLG00000026242 RLG00000026243 RLG00000026244 RLG00000026246 RLG00000026247 RLG00000026248 RLG00000029876 RLG00000030189 RLG00000034579
rosa_multiflora Rmu_co8101374.1_g000001 Rmu_co8309149.1_g000001 Rmu_co8450063.1_g000001 Rmu_sc0000441.1_g000094 Rmu_sc0000479.1_g000005 Rmu_sc0000647.1_g000006 Rmu_sc0001393.1_g000031 Rmu_sc0001423.1_g000037 Rmu_sc0001616.1_g000015 Rmu_sc0001857.1_g000011 Rmu_sc0002183.1_g000013 Rmu_sc0002183.1_g000028 Rmu_sc0002183.1_g000030 Rmu_sc0002688.1_g000003 Rmu_sc0002688.1_g000020 Rmu_sc0003801.1_g000003 Rmu_sc0003801.1_g000004 Rmu_sc0004125.1_g000006 Rmu_sc0004494.1_g000005 Rmu_sc0004494.1_g000006 Rmu_sc0004494.1_g000008 Rmu_sc0004494.1_g000009 Rmu_sc0005842.1_g000004 Rmu_sc0006872.1_g000001 Rmu_sc0006872.1_g000005 Rmu_sc0006872.1_g000009 Rmu_sc0006872.1_g000010 Rmu_sc0008053.1_g000026
rosa_roxburghii Rroxscaffold_159G00432960 Rroxscaffold_1G00033060 Rroxscaffold_2G00123150 Rroxscaffold_2G00123170 Rroxscaffold_2G00133500 Rroxscaffold_2G00133550 Rroxscaffold_4G00278650 Rroxscaffold_4G00278750 Rroxscaffold_4G00278790 Rroxscaffold_4G00278800 Rroxscaffold_4G00278810 Rroxscaffold_4G00325390 Rroxscaffold_5G00354010 Rroxscaffold_6G00395890 Rroxscaffold_6G00395900 Rroxscaffold_6G00395920
rosa_rugosa Rorug01G0046900.1 Rorug01G0427000 Rorug01G0427100 Rorug01G0427200 Rorug01G0427300 Rorug01G0427400 Rorug01G0427500 Rorug01G0427500 Rorug01G0427600 Rorug01G0427600 Rorug01G0427700 Rorug01G0427700 Rorug01G0427800 Rorug01G0428700 Rorug02G0166400 Rorug02G0166500 Rorug02G0166500 Rorug02G0166600 Rorug02G0166600 Rorug02G0166700 Rorug02G0167300 Rorug02G0167300 Rorug02G0167300 Rorug03G0224600 Rorug03G0224600 Rorug04G0094400 Rorug05G0241100 Rorug05G0241200
rosa_samantha Rh1BG407700 Rh1BG407900 Rh1BG409000 Rh1CG064700 Rh1CG420900 Rh1CG421000 Rh1CG421200 Rh1CG421300 Rh1CG421800 Rh1CG421900 Rh1CG422000 Rh1CG422200 Rh1CG422300 Rh1CG423600 Rh1DG437600 Rh1DG438900 Rh1DG439000 Rh1DG440300 Rh2AG219100 Rh2CG220700 Rh2DG225300 Rh3AG274400 Rh3AG274600 Rh3CG308900 Rh3DG305800 Rh4AG156400 Rh5AG129000 Rh5AG320900 Rh5CG139600
rosa_wichuraiana Rw1G005300 Rw1G039280 Rw1G039300 Rw1G039310 Rw1G039320 Rw1G039330 Rw1G039340 Rw1G039360 Rw1G039390 Rw2G016910 Rw2G022800 Rw3G024330 Rw3G024340 Rw3G024350 Rw3G024400 Rw4G012810 Rw5G030120 Rw5G030280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1530
AccB1I GGYRCC 1 cut(s) 1216
AccBSI CCGCTC 1 cut(s) 279
AciI CCGC 4 cut(s) 279, 1373, 1376, 1608
AclWI GGATC 3 cut(s) 53, 66, 1701
AcsI RAATTY 5 cut(s) 192, 676, 868, 991, 1013
AcuI CTGAAG 2 cut(s) 276, 1292
AfaI GTAC 6 cut(s) 536, 555, 883, 963, 1095, 1575
AfiI CCNNNNNNNGG 1 cut(s) 1375
AflII CTTAAG 1 cut(s) 776
AgsI TTSAA 6 cut(s) 242, 587, 921, 1018, 1594, 1673
AjnI CCWGG 1 cut(s) 1368
AloI GAACNNNNNNTCC 2 cut(s) 50, 82
AluBI AGCT 8 cut(s) 203, 305, 380, 390, 1030, 1225, 1247, 1299
AluI AGCT 8 cut(s) 203, 305, 380, 390, 1030, 1225, 1247, 1299
Alw26I GTCTC 3 cut(s) 587, 1455, 1609
AlwI GGATC 3 cut(s) 53, 66, 1701
AlwNI CAGNNNCTG 1 cut(s) 445
Ama87I CYCGRG 1 cut(s) 45
AoxI GGCC 2 cut(s) 928, 1748
ApeKI GCWGC 1 cut(s) 1664
ApoI RAATTY 5 cut(s) 192, 676, 868, 991, 1013
ArsI GACNNNNNNTTYG 2 cut(s) 958, 990
Asp700I GAANNNNTTC 1 cut(s) 818
AspS9I GGNCC 1 cut(s) 1748
AsuC2I CCSGG 2 cut(s) 25, 1747
AsuHPI GGTGA 3 cut(s) 148, 284, 1253
AsuII TTCGAA 1 cut(s) 844
AvaI CYCGRG 1 cut(s) 45
BaeGI GKGCMC 1 cut(s) 1382
BamHI GGATCC 1 cut(s) 58
BanI GGYRCC 1 cut(s) 1216
BanII GRGCYC 1 cut(s) 35
BarI GAAGNNNNNNTAC 4 cut(s) 806, 838, 1557, 1589
BbsI GAAGAC 1 cut(s) 1089
BbvI GCAGC 1 cut(s) 1676
BccI CCATC 5 cut(s) 83, 335, 365, 698, 1072
BceAI ACGGC 1 cut(s) 807
BciT130I CCWGG 1 cut(s) 1370
BcnI CCSGG 2 cut(s) 25, 1747
BcoDI GTCTC 3 cut(s) 587, 1455, 1609
BfaI CTAG 2 cut(s) 393, 1031
BfmI CTRYAG 3 cut(s) 699, 1485, 1599
BfrI CTTAAG 1 cut(s) 776
BfuAI ACCTGC 1 cut(s) 1530
BisI GCNGC 2 cut(s) 1374, 1665
BlpI GCTNAGC 1 cut(s) 1208
BlsI GCNGC 2 cut(s) 1375, 1666
BmcAI AGTACT 1 cut(s) 883
Bme1390I CCNGG 3 cut(s) 25, 1370, 1747
BmeT110I CYCGRG 1 cut(s) 45
BmgT120I GGNCC 1 cut(s) 1748
BmiI GGNNCC 3 cut(s) 60, 562, 1218
BmrFI CCNGG 3 cut(s) 25, 1370, 1747
BmrI ACTGGG 1 cut(s) 433
BmsI GCATC 4 cut(s) 616, 803, 1117, 1499
BmuI ACTGGG 1 cut(s) 433
BpiI GAAGAC 1 cut(s) 1089
Bpu1102I GCTNAGC 1 cut(s) 1208
Bpu14I TTCGAA 1 cut(s) 844
BpuEI CTTGAG 2 cut(s) 1049, 1460
BpuMI CCSGG 2 cut(s) 25, 1747
BsaBI GATNNNNATC 1 cut(s) 920
BsaJI CCNNGG 2 cut(s) 449, 996
Bsc4I CCNNNNNNNGG 1 cut(s) 1375
Bse1I ACTGG 4 cut(s) 439, 502, 1277, 1758
Bse3DI GCAATG 1 cut(s) 1063
Bse8I GATNNNNATC 1 cut(s) 920
BseBI CCWGG 1 cut(s) 1370
BseDI CCNNGG 2 cut(s) 449, 996
BseGI GGATG 3 cut(s) 357, 537, 1053
BseJI GATNNNNATC 1 cut(s) 920
BseLI CCNNNNNNNGG 1 cut(s) 1375
BseMI GCAATG 1 cut(s) 1063
BseMII CTCAG 4 cut(s) 842, 1199, 1466, 1661
BseNI ACTGG 4 cut(s) 439, 502, 1277, 1758
BseRI GAGGAG 2 cut(s) 1013, 1673
BseSI GKGCMC 1 cut(s) 1382
BseXI GCAGC 1 cut(s) 1676
BseYI CCCAGC 1 cut(s) 1741
BsgI GTGCAG 1 cut(s) 726
Bsh1285I CGRYCG 1 cut(s) 1267
BshFI GGCC 2 cut(s) 930, 1750
BshNI GGYRCC 1 cut(s) 1216
BsiEI CGRYCG 1 cut(s) 1267
BsiHKCI CYCGRG 1 cut(s) 45
BsiSI CCGG 3 cut(s) 25, 728, 1746
BslFI GGGAC 1 cut(s) 852
BslI CCNNNNNNNGG 1 cut(s) 1375
BsmAI GTCTC 3 cut(s) 587, 1455, 1609
BsmBI CGTCTC 1 cut(s) 1455
BsmFI GGGAC 1 cut(s) 852
BsmI GAATGC 2 cut(s) 468, 1332
BsnI GGCC 2 cut(s) 930, 1750
BsoBI CYCGRG 1 cut(s) 45
Bsp119I TTCGAA 1 cut(s) 844
Bsp1286I GDGCHC 2 cut(s) 35, 1382
Bsp1407I TGTACA 1 cut(s) 534
Bsp1720I GCTNAGC 1 cut(s) 1208
BspACI CCGC 4 cut(s) 279, 1373, 1376, 1608
BspANI GGCC 2 cut(s) 930, 1750
BspCNI CTCAG 4 cut(s) 843, 1200, 1467, 1660
BspLI GGNNCC 3 cut(s) 60, 562, 1218
BspMI ACCTGC 1 cut(s) 1530
BspPI GGATC 3 cut(s) 53, 66, 1701
BspQI GCTCTTC 1 cut(s) 23
BspT104I TTCGAA 1 cut(s) 844
BspT107I GGYRCC 1 cut(s) 1216
BspTI CTTAAG 1 cut(s) 776
BsrBI CCGCTC 1 cut(s) 279
BsrDI GCAATG 1 cut(s) 1063
BsrGI TGTACA 1 cut(s) 534
BsrI ACTGG 4 cut(s) 439, 502, 1277, 1758
BssECI CCNNGG 2 cut(s) 449, 996
Bst2UI CCWGG 1 cut(s) 1370
Bst4CI ACNGT 1 cut(s) 1600
Bst6I CTCTTC 1 cut(s) 23
BstAFI CTTAAG 1 cut(s) 776
BstAUI TGTACA 1 cut(s) 534
BstBI TTCGAA 1 cut(s) 844
BstC8I GCNNGC 4 cut(s) 547, 616, 932, 1378
BstDEI CTNAG 6 cut(s) 294, 485, 851, 1208, 1475, 1647
BstF5I GGATG 3 cut(s) 357, 537, 1053
BstMAI GTCTC 3 cut(s) 587, 1455, 1609
BstMCI CGRYCG 1 cut(s) 1267
BstMWI GCNNNNNNNGC 1 cut(s) 1673
BstNI CCWGG 1 cut(s) 1370
BstSCI CCNGG 3 cut(s) 23, 1368, 1745
BstSFI CTRYAG 3 cut(s) 699, 1485, 1599
BstSLI GKGCMC 1 cut(s) 1382
BstV1I GCAGC 1 cut(s) 1676
BstV2I GAAGAC 1 cut(s) 1089
BstX2I RGATCY 1 cut(s) 58
BstXI CCANNNNNNTGG 1 cut(s) 557
BstYI RGATCY 1 cut(s) 58
BsuRI GGCC 2 cut(s) 930, 1750
BtsCI GGATG 3 cut(s) 357, 537, 1053
BtsIMutI CAGTG 2 cut(s) 1284, 1751
BveI ACCTGC 1 cut(s) 1530
Cac8I GCNNGC 4 cut(s) 547, 616, 932, 1378
CaiI CAGNNNCTG 1 cut(s) 445
Cfr13I GGNCC 1 cut(s) 1748
CseI GACGC 1 cut(s) 455
Csp6I GTAC 6 cut(s) 535, 554, 882, 962, 1094, 1574
CviAII CATG 7 cut(s) 551, 626, 656, 664, 1198, 1553, 1579
CviQI GTAC 6 cut(s) 535, 554, 882, 962, 1094, 1574
DdeI CTNAG 6 cut(s) 294, 485, 851, 1208, 1475, 1647
Eam1104I CTCTTC 1 cut(s) 23
EarI CTCTTC 1 cut(s) 23
Eco24I GRGCYC 1 cut(s) 35
Eco32I GATATC 1 cut(s) 163
Eco57I CTGAAG 2 cut(s) 276, 1292
Eco88I CYCGRG 1 cut(s) 45
EcoRI GAATTC 1 cut(s) 991
EcoRII CCWGG 1 cut(s) 1368
EcoRV GATATC 1 cut(s) 163
EcoT38I GRGCYC 1 cut(s) 35
Esp3I CGTCTC 1 cut(s) 1455
FaeI CATG 7 cut(s) 554, 629, 659, 667, 1201, 1556, 1582
FaqI GGGAC 1 cut(s) 852
FatI CATG 7 cut(s) 550, 625, 655, 663, 1197, 1552, 1578
FauI CCCGC 1 cut(s) 1369
Fnu4HI GCNGC 2 cut(s) 1374, 1665
FokI GGATG 3 cut(s) 344, 524, 1060
FriOI GRGCYC 1 cut(s) 35
Fsp4HI GCNGC 2 cut(s) 1374, 1665
FspBI CTAG 2 cut(s) 393, 1031
GluI GCNGC 2 cut(s) 1374, 1665
GsaI CCCAGC 1 cut(s) 1745
HaeIII GGCC 2 cut(s) 930, 1750
HapII CCGG 3 cut(s) 25, 728, 1746
HgaI GACGC 1 cut(s) 455
Hin1II CATG 7 cut(s) 554, 629, 659, 667, 1201, 1556, 1582
HindIII AAGCTT 2 cut(s) 1223, 1245
HinfI GANTC 6 cut(s) 48, 415, 667, 759, 770, 1640
HpaII CCGG 3 cut(s) 25, 728, 1746
HphI GGTGA 3 cut(s) 148, 284, 1253
Hpy188I TCNGA 9 cut(s) 149, 852, 915, 997, 1091, 1173, 1236, 1272, 1476
Hpy188III TCNNGA 8 cut(s) 197, 393, 492, 767, 1066, 1289, 1439, 1649
Hpy99I CGWCG 1 cut(s) 1701
HpyAV CCTTC 5 cut(s) 86, 1307, 1343, 1559, 1579
HpyCH4III ACNGT 1 cut(s) 1600
HpyCH4IV ACGT 1 cut(s) 1253
HpyCH4V TGCA 9 cut(s) 105, 618, 743, 874, 908, 1130, 1165, 1197, 1556
HpyF10VI GCNNNNNNNGC 1 cut(s) 1673
HpyF3I CTNAG 6 cut(s) 294, 485, 851, 1208, 1475, 1647
HpySE526I ACGT 1 cut(s) 1253
Hsp92II CATG 7 cut(s) 554, 629, 659, 667, 1201, 1556, 1582
LguI GCTCTTC 1 cut(s) 23
LmnI GCTCC 1 cut(s) 560
Lsp1109I GCAGC 1 cut(s) 1676
LweI GCATC 4 cut(s) 616, 803, 1117, 1499
MaeI CTAG 2 cut(s) 393, 1031
MaeII ACGT 1 cut(s) 1253
MaeIII GTNAC 3 cut(s) 308, 1700, 1734
MbiI CCGCTC 1 cut(s) 279
MflI RGATCY 1 cut(s) 58
MhlI GDGCHC 2 cut(s) 35, 1382
MlyI GAGTC 2 cut(s) 57, 661
MnlI CCTC 7 cut(s) 120, 263, 444, 991, 1154, 1651, 1654
MroXI GAANNNNTTC 1 cut(s) 818
MseI TTAA 7 cut(s) 231, 332, 510, 777, 1182, 1227, 1355
MslI CAYNNNNRTG 4 cut(s) 110, 1101, 1202, 1500
MspCI CTTAAG 1 cut(s) 776
MspI CCGG 3 cut(s) 25, 728, 1746
MspR9I CCNGG 3 cut(s) 25, 1370, 1747
Mva1269I GAATGC 2 cut(s) 468, 1332
MvaI CCWGG 1 cut(s) 1370
MwoI GCNNNNNNNGC 1 cut(s) 1673
NciI CCSGG 2 cut(s) 25, 1747
NlaIII CATG 7 cut(s) 554, 629, 659, 667, 1201, 1556, 1582
NlaIV GGNNCC 3 cut(s) 60, 562, 1218
NmeAIII GCCGAG 1 cut(s) 474
NmuCI GTSAC 1 cut(s) 1734
NspV TTCGAA 1 cut(s) 844
PaeR7I CTCGAG 1 cut(s) 45
PciSI GCTCTTC 1 cut(s) 23
PctI GAATGC 2 cut(s) 468, 1332
PdmI GAANNNNTTC 1 cut(s) 818
PfeI GAWTC 4 cut(s) 415, 759, 770, 1640
PkrI GCNGC 2 cut(s) 1375, 1666
Ple19I CGATCG 1 cut(s) 1267
PleI GAGTC 2 cut(s) 56, 661
PpsI GAGTC 2 cut(s) 56, 661
Psp6I CCWGG 1 cut(s) 1368
PspFI CCCAGC 1 cut(s) 1741
PspGI CCWGG 1 cut(s) 1368
PspN4I GGNNCC 3 cut(s) 60, 562, 1218
PspPI GGNCC 1 cut(s) 1748
PspXI VCTCGAGB 1 cut(s) 45
PstNI CAGNNNCTG 1 cut(s) 445
PsuI RGATCY 1 cut(s) 58
PvuI CGATCG 1 cut(s) 1267
RsaI GTAC 6 cut(s) 536, 555, 883, 963, 1095, 1575
RsaNI GTAC 6 cut(s) 535, 554, 882, 962, 1094, 1574
RseI CAYNNNNRTG 4 cut(s) 110, 1101, 1202, 1500
SapI GCTCTTC 1 cut(s) 23
SaqAI TTAA 7 cut(s) 231, 332, 510, 777, 1182, 1227, 1355
SatI GCNGC 2 cut(s) 1374, 1665
Sau96I GGNCC 1 cut(s) 1748
ScaI AGTACT 1 cut(s) 883
SchI GAGTC 2 cut(s) 57, 661
ScrFI CCNGG 3 cut(s) 25, 1370, 1747
SduI GDGCHC 2 cut(s) 35, 1382
SfaNI GCATC 4 cut(s) 616, 803, 1117, 1499
SfcI CTRYAG 3 cut(s) 699, 1485, 1599
Sfr274I CTCGAG 1 cut(s) 45
SfuI TTCGAA 1 cut(s) 844
SlaI CTCGAG 1 cut(s) 45
SmiMI CAYNNNNRTG 4 cut(s) 110, 1101, 1202, 1500
SmlI CTYRAG 4 cut(s) 45, 776, 1064, 1439
SmoI CTYRAG 4 cut(s) 45, 776, 1064, 1439
SsiI CCGC 4 cut(s) 279, 1373, 1376, 1608
SspI AATATT 3 cut(s) 123, 181, 710
SspMI CTAG 2 cut(s) 393, 1031
StyD4I CCNGG 3 cut(s) 23, 1368, 1745
TaaI ACNGT 1 cut(s) 1600
TaiI ACGT 1 cut(s) 1256
TaqI TCGA 6 cut(s) 46, 268, 336, 460, 844, 977
TatI WGTACW 6 cut(s) 534, 553, 881, 961, 1093, 1573
TauI GCSGC 1 cut(s) 1376
TfiI GAWTC 4 cut(s) 415, 759, 770, 1640
Tru1I TTAA 7 cut(s) 231, 332, 510, 777, 1182, 1227, 1355
Tru9I TTAA 7 cut(s) 231, 332, 510, 777, 1182, 1227, 1355
TscAI CASTG 2 cut(s) 1284, 1758
TseFI GTSAC 1 cut(s) 1734
TseI GCWGC 1 cut(s) 1664
Tsp45I GTSAC 1 cut(s) 1734
TspDTI ATGAA 9 cut(s) 160, 462, 507, 672, 750, 995, 1064, 1119, 1330
TspGWI ACGGA 2 cut(s) 1361, 1473
TspRI CASTG 2 cut(s) 1284, 1758
Vha464I CTTAAG 1 cut(s) 776
XapI RAATTY 5 cut(s) 192, 676, 868, 991, 1013
XbaI TCTAGA 1 cut(s) 392
XhoI CTCGAG 1 cut(s) 45
XmnI GAANNNNTTC 1 cut(s) 818
XspI CTAG 2 cut(s) 393, 1031
ZrmI AGTACT 1 cut(s) 883
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.