Rw2G016910

Cyclic nucleotide-gated ion channel 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000119
Physical Location & Seq
Reverse (-)
20442560 .. 20447138
4579 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G016910.1

Sequence Viewer

Length: 1884 bp
ATGACGAGTAATCCAGATAATCTGGCAGATCCAAATCAACAAAGCCTTACACCAAGAGCTTCAGCAGCACACCCAATTAAGCAAAAAAGGAGAATTGCTGTGATTTTATATCCATGTTGGCGACCAATTTTTGTAATCTCATGTGTGATTGCAGTTTCAGTCGATCCTTTATTCTTCTACCTCCCAGTCATCGACGAGAAAAACAAATGCCTTGGAATGGACAAGACGTTGAAGGTGGTTGCCCTTGTCTTGCGATCACTCACGGATGTCACTTTGATAGTGAACATTATATATCAGTTTTGTAAGGCTATTAAAGCTGCTATGAAATCAAGAGACGAACAAAAGAAGAAAAAAGAGGCACAAACAAGAGGAAAGAAGGATAAAAAAGCAGGTGGAAAGTCAGTTTGCGAATGGAACGAGATAGTTCCATTTGCCAAGGCATTAGCTCAGAAGTTGTCATGGCGCTCCATCATAATCGACTTCTTTGCTGTTCTTCCGATTCCTCAGGTGCTAATAGTAGCTGCTGCTTCCAAAATGAGAAGCTCGAAATATATAGACAAAAAAAAGACTCTGAATTTCTTTCTTCTTGCTCAATATTTGCCAAGGGTCTACCGAATGCACCATTCGTCTATAGAACTTAGGAGGAAGACTGGAATATGGACTAAAGGTGCCTTCAATTTTTTCTTATACATCCTTGCCAGTCACGTACTTGGAGCCTTTTGGTATTTCTTCTCTATTCAACGAGAGACGTCATGTTGGCACCAAGCGTGTGTAAAACATAGTAACACAAGATGTAATTATTATTGTGATGAAGACACGGCATCAAGAAATATGACGTTCATCAATTCTCTTGATGAATTTTGCCTTGTAGATGTGCCAGAAAATGCTACAGCACCCTTCGATTTTGGAATATTTCTTGATTCCCTTAAGACTGGTAATGCAGGGTCAATAGATTTCAGAAAAAAGTTTTTGTACTCTTTTTGGTGGGGCCTACGAAATCTAAGTAATTTTGGCACAAATCTAGAAACAAGTACGTACGTGTGGGAAAACTGCTTCGCTATTCTTATTTCTGTCATTGGCTTGCTACTATTTCTATATCTTATTGGGAAGGTGCAGACATTTATCTCGATGCGAACTCAAAAATCGGAGGAGAGAGATAAGAAGCTTAAAATGAAAAAGCTAGGTATACAAGTTTGGATGAACAAACACGGTTTCACAGATGAAATGAAGCTGGAAATGAAGGAACATGTAAAACGAAATTGGGAAGATATCAAAGATGCTGACATGGAGAATTTATTCTCTATTCTTCCGGGAAAAACCAGAAAAGTCCTGAAGGAACATATCTGCATGGAAATGCTTAAAAATGTCCCAAAGCTTGCAAATATGGATGTAAAAGTGTTGAGGATGATCTGCGAATATCTAATTCCAGTCAGCTACCTGGATCATAGCTTCATTTTTCGAACAGAAGAACCAATGGATCGCATGCTCTTCATTACAAAAGGCTTCGTGTTGACCTCCCCGACTAGTGATAGTAACCCTTCAGTGATCGAGAAGAGGTCCCTGAAAAGTGGTGGTTTCTATGGAGACGAAGAGCTTCTGACTTGGGTAGCATCCAAAGAAAATCCCTGTCTTTCTGACCTTCCTACCTTAAAGGAAAATGTCAAATGTCATTCAAAGGTAGATTGCTTTGTTCTCTTGGCCAAGGATTTGAGAACAGTAGTTTCCAGATCCAGAGTCTATTGGGATCTGAACAATTCTGAAAAAAAAGAAGAGGTAGCAAAAGCAGCAATCCTTCCATTCCTTCGTCTCCGCCTTGATCATCAAAGAGCGGGTGCTCAGAACGGAAACAGCAGCAATCACATAGTAATAGAAACCTCTACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
Ion_trans

Protein Analysis

627

Amino Acids

72.16

Weight (kDa)

9.37

Isoelectric Point (pI)

36.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ion_trans PF00520 154 - 382 2.3e-07 Ion transport protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000119)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g10570 FvH4_4g36390 FvH4_7g31840 FvH4_7g31840 FvH4_7g31850 FvH4_7g31850 FvH4_7g31861 FvH4_7g31861 FvH4_7g31950
malus_domestica MD01G1054600.v1.1 MD01G1221100.v1.1 MD01G1221400.v1.1 MD01G1221600.v1.1 MD01G1221800.v1.1 MD01G1222000.v1.1 MD01G1222100.v1.1 MD07G1140800.v1.1 MD07G1291200.v1.1 MD07G1291400.v1.1 MD07G1291500.v1.1 MD07G1291700.v1.1 MD07G1292500.v1.1 MD07G1292700.v1.1 MD07G1292800.v1.1 MD07G1292900.v1.1 MD08G1241600.v1.1 MD11G1083800.v1.1 MD15G1431600.v1.1
prunus_persica Prupe.1G017800_v2.0.a1 Prupe.1G017800_v2.0.a1 Prupe.1G162800_v2.0.a1 Prupe.2G312500_v2.0.a1 Prupe.2G312500_v2.0.a1 Prupe.2G312700_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092800_v2.0.a1 Prupe.4G092800_v2.0.a1 Prupe.6G142800_v2.0.a1 Prupe.6G142800_v2.0.a1 Prupe.6G143000_v2.0.a1 Prupe.6G143100_v2.0.a1 Prupe.6G143100_v2.0.a1 Prupe.6G143100_v2.0.a1 Prupe.6G143100_v2.0.a1
pyrus_communis pycom01g23050 pycom01g23070 pycom01g23090 pycom01g23100 pycom01g23110 pycom01g23120 pycom01g23170 pycom07g26410 pycom07g26440 pycom07g26470 pycom07g26480 pycom07g26490 pycom07g26500 pycom07g26510 pycom07g26530 pycom07g26540 pycom07g26570 pycom07g26580 pycom07g26590 pycom08g20940
rosa_chinensis RchiOBHm_Chr1g0324571 RchiOBHm_Chr1g0336181 RchiOBHm_Chr1g0381061 RchiOBHm_Chr1g0381091 RchiOBHm_Chr1g0381121 RchiOBHm_Chr1g0381131 RchiOBHm_Chr1g0381141 RchiOBHm_Chr1g0381161 RchiOBHm_Chr1g0381211 RchiOBHm_Chr2g0110361 RchiOBHm_Chr2g0120231 RchiOBHm_Chr3g0486281 RchiOBHm_Chr3g0486311 RchiOBHm_Chr3g0486401 RchiOBHm_Chr4g0409511 RchiOBHm_Chr4g0409521 RchiOBHm_Chr5g0017271 RchiOBHm_Chr5g0048781 RchiOBHm_Chr5g0048811
rosa_laevigata RLG00000002401 RLG00000008559 RLG00000017798 RLG00000017799 RLG00000023082 RLG00000023088 RLG00000026230 RLG00000026238 RLG00000026240 RLG00000026241 RLG00000026242 RLG00000026243 RLG00000026244 RLG00000026246 RLG00000026247 RLG00000026248 RLG00000029876 RLG00000030189 RLG00000034579
rosa_multiflora Rmu_co8101374.1_g000001 Rmu_co8309149.1_g000001 Rmu_co8450063.1_g000001 Rmu_sc0000441.1_g000094 Rmu_sc0000479.1_g000005 Rmu_sc0000647.1_g000006 Rmu_sc0001393.1_g000031 Rmu_sc0001423.1_g000037 Rmu_sc0001616.1_g000015 Rmu_sc0001857.1_g000011 Rmu_sc0002183.1_g000013 Rmu_sc0002183.1_g000028 Rmu_sc0002183.1_g000030 Rmu_sc0002688.1_g000003 Rmu_sc0002688.1_g000020 Rmu_sc0003801.1_g000003 Rmu_sc0003801.1_g000004 Rmu_sc0004125.1_g000006 Rmu_sc0004494.1_g000005 Rmu_sc0004494.1_g000006 Rmu_sc0004494.1_g000008 Rmu_sc0004494.1_g000009 Rmu_sc0005842.1_g000004 Rmu_sc0006872.1_g000001 Rmu_sc0006872.1_g000005 Rmu_sc0006872.1_g000009 Rmu_sc0006872.1_g000010 Rmu_sc0008053.1_g000026
rosa_roxburghii Rroxscaffold_159G00432960 Rroxscaffold_1G00033060 Rroxscaffold_2G00123150 Rroxscaffold_2G00123170 Rroxscaffold_2G00133500 Rroxscaffold_2G00133550 Rroxscaffold_4G00278650 Rroxscaffold_4G00278750 Rroxscaffold_4G00278790 Rroxscaffold_4G00278800 Rroxscaffold_4G00278810 Rroxscaffold_4G00325390 Rroxscaffold_5G00354010 Rroxscaffold_6G00395890 Rroxscaffold_6G00395900 Rroxscaffold_6G00395920
rosa_rugosa Rorug01G0046900.1 Rorug01G0427000 Rorug01G0427100 Rorug01G0427200 Rorug01G0427300 Rorug01G0427400 Rorug01G0427500 Rorug01G0427500 Rorug01G0427600 Rorug01G0427600 Rorug01G0427700 Rorug01G0427700 Rorug01G0427800 Rorug01G0428700 Rorug02G0166400 Rorug02G0166500 Rorug02G0166500 Rorug02G0166600 Rorug02G0166600 Rorug02G0166700 Rorug02G0167300 Rorug02G0167300 Rorug02G0167300 Rorug03G0224600 Rorug03G0224600 Rorug04G0094400 Rorug05G0241100 Rorug05G0241200
rosa_samantha Rh1BG407700 Rh1BG407900 Rh1BG409000 Rh1CG064700 Rh1CG420900 Rh1CG421000 Rh1CG421200 Rh1CG421300 Rh1CG421800 Rh1CG421900 Rh1CG422000 Rh1CG422200 Rh1CG422300 Rh1CG423600 Rh1DG437600 Rh1DG438900 Rh1DG439000 Rh1DG440300 Rh2AG219100 Rh2CG220700 Rh2DG225300 Rh3AG274400 Rh3AG274600 Rh3CG308900 Rh3DG305800 Rh4AG156400 Rh5AG129000 Rh5AG320900 Rh5CG139600
rosa_wichuraiana Rw1G005300 Rw1G039280 Rw1G039300 Rw1G039310 Rw1G039320 Rw1G039330 Rw1G039340 Rw1G039360 Rw1G039390 Rw2G016910 Rw2G022800 Rw3G024330 Rw3G024340 Rw3G024350 Rw3G024400 Rw4G012810 Rw5G030120 Rw5G030280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 380
AatII GACGTC 1 cut(s) 752
Acc36I ACCTGC 1 cut(s) 380
AccB1I GGYRCC 2 cut(s) 668, 759
AccBSI CCGCTC 1 cut(s) 1829
AccI GTMKAC 2 cut(s) 609, 1186
AciI CCGC 2 cut(s) 1810, 1829
AclWI GGATC 6 cut(s) 23, 158, 1449, 1485, 1722, 1752
AcoI YGGCCR 1 cut(s) 1698
AcsI RAATTY 3 cut(s) 574, 857, 1291
AcuI CTGAAG 3 cut(s) 45, 1352, 1524
AcyI GRCGYC 1 cut(s) 749
AfaI GTAC 4 cut(s) 708, 974, 1033, 1037
AfiI CCNNNNNNNGG 1 cut(s) 217
AflII CTTAAG 1 cut(s) 926
AflIII ACRYGT 2 cut(s) 1038, 1246
AgsI TTSAA 4 cut(s) 232, 676, 740, 1674
AhlI ACTAGT 1 cut(s) 1523
AjnI CCWGG 1 cut(s) 1437
Alw21I GWGCWC 1 cut(s) 1837
Alw26I GTCTC 4 cut(s) 327, 740, 1578, 1811
AlwI GGATC 6 cut(s) 23, 158, 1449, 1485, 1722, 1752
AoxI GGCC 2 cut(s) 988, 1698
ApeKI GCWGC 6 cut(s) 65, 317, 521, 524, 1784, 1851
ApoI RAATTY 3 cut(s) 574, 857, 1291
Asp700I GAANNNNTTC 2 cut(s) 1295, 1593
AspLEI GCGC 1 cut(s) 465
AspS9I GGNCC 2 cut(s) 988, 1557
AsuC2I CCSGG 1 cut(s) 1311
AsuII TTCGAA 1 cut(s) 1459
AvaII GGWCC 1 cut(s) 1557
AxyI CCTNAGG 1 cut(s) 504
BalI TGGCCA 1 cut(s) 1700
BanI GGYRCC 2 cut(s) 668, 759
BbsI GAAGAC 2 cut(s) 653, 819
Bbv12I GWGCWC 1 cut(s) 1837
BbvI GCAGC 6 cut(s) 77, 304, 508, 511, 1796, 1863
BccI CCATC 1 cut(s) 476
BceAI ACGGC 1 cut(s) 834
BcgI CGANNNNNNTGC 2 cut(s) 467, 501
BciT130I CCWGG 1 cut(s) 1439
BclI TGATCA 1 cut(s) 1816
BcnI CCSGG 1 cut(s) 1311
BcoDI GTCTC 4 cut(s) 327, 740, 1578, 1811
BcuI ACTAGT 1 cut(s) 1523
BfaI CTAG 3 cut(s) 1022, 1181, 1524
BfmI CTRYAG 2 cut(s) 630, 888
BfoI RGCGCY 1 cut(s) 466
BfrI CTTAAG 1 cut(s) 926
BfuAI ACCTGC 1 cut(s) 380
BisI GCNGC 6 cut(s) 66, 318, 522, 525, 1785, 1852
BlsI GCNGC 6 cut(s) 67, 319, 523, 526, 1786, 1853
Bme1390I CCNGG 2 cut(s) 1311, 1439
Bme18I GGWCC 1 cut(s) 1557
BmgT120I GGNCC 2 cut(s) 988, 1557
BmiI GGNNCC 5 cut(s) 670, 715, 761, 989, 1559
BmrFI CCNGG 2 cut(s) 1311, 1439
BmrI ACTGGG 1 cut(s) 179
BmsI GCATC 4 cut(s) 830, 1119, 1267, 1619
BmuI ACTGGG 1 cut(s) 179
BpiI GAAGAC 2 cut(s) 653, 819
Bpu14I TTCGAA 1 cut(s) 1459
BpuMI CCSGG 1 cut(s) 1311
BsaAI YACGTR 3 cut(s) 706, 1035, 1039
BsaBI GATNNNNATC 1 cut(s) 33
BsaHI GRCGYC 1 cut(s) 749
BsaJI CCNNGG 4 cut(s) 211, 435, 602, 1701
Bsc4I CCNNNNNNNGG 1 cut(s) 217
Bse1I ACTGG 5 cut(s) 185, 655, 699, 937, 1427
Bse21I CCTNAGG 1 cut(s) 504
Bse8I GATNNNNATC 1 cut(s) 33
BseBI CCWGG 1 cut(s) 1439
BseDI CCNNGG 4 cut(s) 211, 435, 602, 1701
BseGI GGATG 6 cut(s) 271, 690, 1203, 1393, 1410, 1610
BseJI GATNNNNATC 1 cut(s) 33
BseLI CCNNNNNNNGG 1 cut(s) 217
BseMII CTCAG 3 cut(s) 461, 518, 1850
BseNI ACTGG 5 cut(s) 185, 655, 699, 937, 1427
BseRI GAGGAG 1 cut(s) 1163
BseXI GCAGC 6 cut(s) 77, 304, 508, 511, 1796, 1863
BsgI GTGCAG 1 cut(s) 1133
BshFI GGCC 2 cut(s) 990, 1700
BshNI GGYRCC 2 cut(s) 668, 759
BsiHKAI GWGCWC 1 cut(s) 1837
BsiSI CCGG 1 cut(s) 1310
BsiWI CGTACG 1 cut(s) 1035
BslFI GGGAC 2 cut(s) 1352, 1543
BslI CCNNNNNNNGG 1 cut(s) 217
BsmAI GTCTC 4 cut(s) 327, 740, 1578, 1811
BsmBI CGTCTC 4 cut(s) 327, 740, 1578, 1811
BsmFI GGGAC 2 cut(s) 1352, 1543
BsmI GAATGC 1 cut(s) 621
BsnI GGCC 2 cut(s) 990, 1700
Bsp119I TTCGAA 1 cut(s) 1459
Bsp1286I GDGCHC 1 cut(s) 1837
BspACI CCGC 2 cut(s) 1810, 1829
BspANI GGCC 2 cut(s) 990, 1700
BspCNI CTCAG 3 cut(s) 460, 517, 1849
BspLI GGNNCC 5 cut(s) 670, 715, 761, 989, 1559
BspMI ACCTGC 1 cut(s) 380
BspPI GGATC 6 cut(s) 23, 158, 1449, 1485, 1722, 1752
BspQI GCTCTTC 2 cut(s) 1493, 1584
BspT104I TTCGAA 1 cut(s) 1459
BspT107I GGYRCC 2 cut(s) 668, 759
BspTI CTTAAG 1 cut(s) 926
BsrBI CCGCTC 1 cut(s) 1829
BsrI ACTGG 5 cut(s) 185, 655, 699, 937, 1427
BssECI CCNNGG 4 cut(s) 211, 435, 602, 1701
BssNAI GTATAC 1 cut(s) 1187
BssNI GRCGYC 1 cut(s) 749
BssT1I CCWWGG 4 cut(s) 211, 435, 602, 1701
Bst1107I GTATAC 1 cut(s) 1187
Bst2UI CCWGG 1 cut(s) 1439
Bst4CI ACNGT 2 cut(s) 1211, 1717
Bst6I CTCTTC 4 cut(s) 1493, 1547, 1584, 1764
BstACI GRCGYC 1 cut(s) 749
BstAFI CTTAAG 1 cut(s) 926
BstBAI YACGTR 3 cut(s) 706, 1035, 1039
BstBI TTCGAA 1 cut(s) 1459
BstC8I GCNNGC 3 cut(s) 1082, 1377, 1484
BstDEI CTNAG 5 cut(s) 447, 504, 638, 1001, 1836
BstF5I GGATG 6 cut(s) 271, 690, 1203, 1393, 1410, 1610
BstH2I RGCGCY 1 cut(s) 466
BstHHI GCGC 1 cut(s) 465
BstMAI GTCTC 4 cut(s) 327, 740, 1578, 1811
BstMWI GCNNNNNNNGC 3 cut(s) 65, 314, 1784
BstNI CCWGG 1 cut(s) 1439
BstNSI RCATGY 2 cut(s) 1250, 1486
BstSCI CCNGG 2 cut(s) 1309, 1437
BstSFI CTRYAG 2 cut(s) 630, 888
BstSNI TACGTA 1 cut(s) 1035
BstV1I GCAGC 6 cut(s) 77, 304, 508, 511, 1796, 1863
BstV2I GAAGAC 2 cut(s) 653, 819
BstX2I RGATCY 3 cut(s) 28, 1727, 1744
BstYI RGATCY 3 cut(s) 28, 1727, 1744
BstZ17I GTATAC 1 cut(s) 1187
Bsu36I CCTNAGG 1 cut(s) 504
BsuRI GGCC 2 cut(s) 990, 1700
BtsCI GGATG 6 cut(s) 271, 690, 1203, 1393, 1410, 1610
BtsIMutI CAGTG 1 cut(s) 1548
BveI ACCTGC 1 cut(s) 380
Cac8I GCNNGC 3 cut(s) 1082, 1377, 1484
CfoI GCGC 1 cut(s) 465
Cfr13I GGNCC 2 cut(s) 988, 1557
Csp6I GTAC 4 cut(s) 707, 973, 1032, 1036
CviAII CATG 8 cut(s) 114, 141, 459, 753, 1247, 1285, 1348, 1483
CviQI GTAC 4 cut(s) 707, 973, 1032, 1036
DdeI CTNAG 5 cut(s) 447, 504, 638, 1001, 1836
EaeI YGGCCR 1 cut(s) 1698
Eam1104I CTCTTC 4 cut(s) 1493, 1547, 1584, 1764
EarI CTCTTC 4 cut(s) 1493, 1547, 1584, 1764
EciI GGCGGA 1 cut(s) 1799
Eco105I TACGTA 1 cut(s) 1035
Eco130I CCWWGG 4 cut(s) 211, 435, 602, 1701
Eco32I GATATC 1 cut(s) 1270
Eco47I GGWCC 1 cut(s) 1557
Eco57I CTGAAG 3 cut(s) 45, 1352, 1524
Eco81I CCTNAGG 1 cut(s) 504
EcoO109I RGGNCCY 2 cut(s) 988, 1557
EcoRII CCWGG 1 cut(s) 1437
EcoRV GATATC 1 cut(s) 1270
EcoT14I CCWWGG 4 cut(s) 211, 435, 602, 1701
ErhI CCWWGG 4 cut(s) 211, 435, 602, 1701
Esp3I CGTCTC 4 cut(s) 327, 740, 1578, 1811
FaeI CATG 8 cut(s) 117, 144, 462, 756, 1250, 1288, 1351, 1486
FaqI GGGAC 2 cut(s) 1352, 1543
FatI CATG 8 cut(s) 113, 140, 458, 752, 1246, 1284, 1347, 1482
FauI CCCGC 1 cut(s) 1822
FbaI TGATCA 1 cut(s) 1816
FblI GTMKAC 2 cut(s) 609, 1186
Fnu4HI GCNGC 6 cut(s) 66, 318, 522, 525, 1785, 1852
FokI GGATG 6 cut(s) 278, 677, 1210, 1400, 1417, 1597
Fsp4HI GCNGC 6 cut(s) 66, 318, 522, 525, 1785, 1852
FspBI CTAG 3 cut(s) 1022, 1181, 1524
GlaI GCGC 1 cut(s) 464
GluI GCNGC 6 cut(s) 66, 318, 522, 525, 1785, 1852
HaeII RGCGCY 1 cut(s) 466
HaeIII GGCC 2 cut(s) 990, 1700
HapII CCGG 1 cut(s) 1310
HhaI GCGC 1 cut(s) 465
Hin1I GRCGYC 1 cut(s) 749
Hin1II CATG 8 cut(s) 117, 144, 462, 756, 1250, 1288, 1351, 1486
Hin6I GCGC 1 cut(s) 463
HinP1I GCGC 1 cut(s) 463
HincII GTYRAC 1 cut(s) 1512
HindII GTYRAC 1 cut(s) 1512
HindIII AAGCTT 2 cut(s) 1163, 1373
HinfI GANTC 4 cut(s) 499, 568, 920, 1734
HpaII CCGG 1 cut(s) 1310
Hpy166II GTNNAC 4 cut(s) 283, 610, 1187, 1512
Hpy8I GTNNAC 4 cut(s) 283, 610, 1187, 1512
Hpy99I CGWCG 1 cut(s) 197
HpyCH4III ACNGT 2 cut(s) 1211, 1717
HpyCH4IV ACGT 6 cut(s) 227, 705, 749, 836, 1034, 1038
HpyCH4V TGCA 6 cut(s) 152, 619, 941, 1114, 1347, 1379
HpyF10VI GCNNNNNNNGC 3 cut(s) 65, 314, 1784
HpyF3I CTNAG 5 cut(s) 447, 504, 638, 1001, 1836
HpySE526I ACGT 6 cut(s) 227, 705, 749, 836, 1034, 1038
Hsp92I GRCGYC 1 cut(s) 749
Hsp92II CATG 8 cut(s) 117, 144, 462, 756, 1250, 1288, 1351, 1486
HspAI GCGC 1 cut(s) 463
Ksp22I TGATCA 1 cut(s) 1816
LguI GCTCTTC 2 cut(s) 1493, 1584
LmnI GCTCC 2 cut(s) 470, 713
Lsp1109I GCAGC 6 cut(s) 77, 304, 508, 511, 1796, 1863
LweI GCATC 4 cut(s) 830, 1119, 1267, 1619
MaeI CTAG 3 cut(s) 1022, 1181, 1524
MaeII ACGT 6 cut(s) 227, 705, 749, 836, 1034, 1038
MaeIII GTNAC 4 cut(s) 268, 701, 782, 1532
MbiI CCGCTC 1 cut(s) 1829
MflI RGATCY 3 cut(s) 28, 1727, 1744
MhlI GDGCHC 1 cut(s) 1837
MlsI TGGCCA 1 cut(s) 1700
MluNI TGGCCA 1 cut(s) 1700
MlyI GAGTC 2 cut(s) 562, 1743
Mox20I TGGCCA 1 cut(s) 1700
MroXI GAANNNNTTC 2 cut(s) 1295, 1593
MscI TGGCCA 1 cut(s) 1700
MseI TTAA 6 cut(s) 78, 312, 927, 1167, 1359, 1649
MslI CAYNNNNRTG 1 cut(s) 1352
Msp20I TGGCCA 1 cut(s) 1700
MspCI CTTAAG 1 cut(s) 926
MspI CCGG 1 cut(s) 1310
MspR9I CCNGG 2 cut(s) 1311, 1439
Mva1269I GAATGC 1 cut(s) 621
MvaI CCWGG 1 cut(s) 1439
MwoI GCNNNNNNNGC 3 cut(s) 65, 314, 1784
NciI CCSGG 1 cut(s) 1311
NlaIII CATG 8 cut(s) 117, 144, 462, 756, 1250, 1288, 1351, 1486
NlaIV GGNNCC 5 cut(s) 670, 715, 761, 989, 1559
NmuCI GTSAC 2 cut(s) 268, 701
NspI RCATGY 2 cut(s) 1250, 1486
NspV TTCGAA 1 cut(s) 1459
PaeI GCATGC 1 cut(s) 1486
PaqCI CACCTGC 1 cut(s) 380
PciI ACATGT 1 cut(s) 1246
PciSI GCTCTTC 2 cut(s) 1493, 1584
PctI GAATGC 1 cut(s) 621
PdmI GAANNNNTTC 2 cut(s) 1295, 1593
PfeI GAWTC 2 cut(s) 499, 920
Pfl23II CGTACG 1 cut(s) 1035
PfoI TCCNGGA 1 cut(s) 1309
PkrI GCNGC 6 cut(s) 67, 319, 523, 526, 1786, 1853
PleI GAGTC 2 cut(s) 562, 1742
PpsI GAGTC 2 cut(s) 562, 1742
Ppu21I YACGTR 3 cut(s) 706, 1035, 1039
PpuMI RGGWCCY 1 cut(s) 1557
PscI ACATGT 1 cut(s) 1246
Psp5II RGGWCCY 1 cut(s) 1557
Psp6I CCWGG 1 cut(s) 1437
PspGI CCWGG 1 cut(s) 1437
PspLI CGTACG 1 cut(s) 1035
PspN4I GGNNCC 5 cut(s) 670, 715, 761, 989, 1559
PspPI GGNCC 2 cut(s) 988, 1557
PspPPI RGGWCCY 1 cut(s) 1557
PsuI RGATCY 3 cut(s) 28, 1727, 1744
RsaI GTAC 4 cut(s) 708, 974, 1033, 1037
RsaNI GTAC 4 cut(s) 707, 973, 1032, 1036
RseI CAYNNNNRTG 1 cut(s) 1352
SapI GCTCTTC 2 cut(s) 1493, 1584
SaqAI TTAA 6 cut(s) 78, 312, 927, 1167, 1359, 1649
SatI GCNGC 6 cut(s) 66, 318, 522, 525, 1785, 1852
Sau96I GGNCC 2 cut(s) 988, 1557
SchI GAGTC 2 cut(s) 562, 1743
ScrFI CCNGG 2 cut(s) 1311, 1439
SduI GDGCHC 1 cut(s) 1837
SfaNI GCATC 4 cut(s) 830, 1119, 1267, 1619
SfcI CTRYAG 2 cut(s) 630, 888
SfuI TTCGAA 1 cut(s) 1459
SinI GGWCC 1 cut(s) 1557
SmiMI CAYNNNNRTG 1 cut(s) 1352
SmlI CTYRAG 1 cut(s) 926
SmoI CTYRAG 1 cut(s) 926
SnaBI TACGTA 1 cut(s) 1035
SpeI ACTAGT 1 cut(s) 1523
SphI GCATGC 1 cut(s) 1486
SsiI CCGC 2 cut(s) 1810, 1829
SspI AATATT 2 cut(s) 596, 912
SspMI CTAG 3 cut(s) 1022, 1181, 1524
StyD4I CCNGG 2 cut(s) 1309, 1437
StyI CCWWGG 4 cut(s) 211, 435, 602, 1701
TaaI ACNGT 2 cut(s) 1211, 1717
TaiI ACGT 6 cut(s) 230, 708, 752, 839, 1037, 1041
TaqI TCGA 8 cut(s) 162, 192, 477, 545, 900, 1127, 1459, 1548
TatI WGTACW 1 cut(s) 972
TfiI GAWTC 2 cut(s) 499, 920
Tru1I TTAA 6 cut(s) 78, 312, 927, 1167, 1359, 1649
Tru9I TTAA 6 cut(s) 78, 312, 927, 1167, 1359, 1649
TscAI CASTG 1 cut(s) 1548
TseFI GTSAC 2 cut(s) 268, 701
TseI GCWGC 6 cut(s) 65, 317, 521, 524, 1784, 1851
Tsp45I GTSAC 2 cut(s) 268, 701
TspGWI ACGGA 2 cut(s) 278, 1857
TspRI CASTG 1 cut(s) 1548
Vha464I CTTAAG 1 cut(s) 926
VpaK11BI GGWCC 1 cut(s) 1557
XapI RAATTY 3 cut(s) 574, 857, 1291
XbaI TCTAGA 1 cut(s) 1021
XceI RCATGY 2 cut(s) 1250, 1486
XmiI GTMKAC 2 cut(s) 609, 1186
XmnI GAANNNNTTC 2 cut(s) 1295, 1593
XspI CTAG 3 cut(s) 1022, 1181, 1524
ZraI GACGTC 1 cut(s) 750
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.