Rh3CG308900

Cyclic nucleotide-gated ion channel 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0000119
Physical Location & Seq
Reverse (-)
32433019 .. 32442528
9510 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG308900.1

Sequence Viewer

Length: 1188 bp
ATGTCTAACAAGCAGGTACCCAATGATGTCCCTATGATGAGCATCACACGGGGAATTAAGTACAATTTCGATAATGTTGAGGCGCAACCGCCAGATCCCCCTTGGAGAGATCGAGATGCAGTTTCAGCAAAACAAAAATTGGAAGGAACACTTCTTTCAGTTTCATTCTGGAATAAGATGTTTGTAATTGCATGTGTGATTGCAGTCTCATTAGACCCATTGTTCTTCTACATTCCAATCATCGATGAAGGAAAAAAGTGCCTTGCAGAAGACAAAGAGTTGAGGACTATAGCTCTCATTTTGCGATCGCTTACTGATGTCACTTTCGTAGTGAATATCATATATCATGTTTGCAAAGCCATTAACGCTGCCTACAAAGAACACAAAGGAAAAAAGTCTGAACTGGCATCGGATTGGGAATTTTCGAAGGTATCAGCAGCGGAGATAATTCCATTTGCTAAATTAGTTGCTGGGAAGCTAGCATGGCGCTCTACGCTAACCAGCATTTTAGCTGTTTTTCCCATGCCACAACTGCTTCTAGTATTTGTCTTCTTCAAAATGAGAGGCCATGCATACTTGCAGCATAGAAAGATCCTGAATGTATTTCTTCTCGCTCAATATCTGCCAAAGATCTATCGAATTTACCTATCATCTCAGAAACTCAGACAAACTACTGGATTATGGGCTAAAGCTCTGTTTAACTTTTTTCTATATATCCTTGCAAGTCATGTACTTGGAGCTTTTTGGTACTTTTTTTCCATTCAACGAGAAACTTCTTGTTGGCTTCGGGCATGTGTAAATAATAGTCCAAATGTTGAAGAATGTATGAGTACCTTATACTGCGAGGTTCACAATACTGATGCAAGAAATGCCACATTCCTTAATCAATTTTGCCCTATAAGTGCTGAAGAGAATGCTAAGGCCCCTTTTGATTTTGGAATATTTCTGGATTCCCTCAAAAATGGTATCACAGGAGATATACATTTCGGAACGAAGTTATCTTACTCTTTTTGGTGGGGATTACGTAATCTAAGTAATTTTGGCACAAATCTCGAAACAAGCACCTATGTGTGGGAAACTTGCTTTGCAGTTCTTATTTCTGTGATTGGCTTGCTACTATTTCTATATCTCATTGGACATGTACAGGTTGGTTATGAATCTCTCTATTGGTACTGTATCTTTGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
Ion_trans

Protein Analysis

395

Amino Acids

45.26

Weight (kDa)

8.67

Isoelectric Point (pI)

37.88

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000119)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g10570 FvH4_4g36390 FvH4_7g31840 FvH4_7g31840 FvH4_7g31850 FvH4_7g31850 FvH4_7g31861 FvH4_7g31861 FvH4_7g31950
malus_domestica MD01G1054600.v1.1 MD01G1221100.v1.1 MD01G1221400.v1.1 MD01G1221600.v1.1 MD01G1221800.v1.1 MD01G1222000.v1.1 MD01G1222100.v1.1 MD07G1140800.v1.1 MD07G1291200.v1.1 MD07G1291400.v1.1 MD07G1291500.v1.1 MD07G1291700.v1.1 MD07G1292500.v1.1 MD07G1292700.v1.1 MD07G1292800.v1.1 MD07G1292900.v1.1 MD08G1241600.v1.1 MD11G1083800.v1.1 MD15G1431600.v1.1
prunus_persica Prupe.1G017800_v2.0.a1 Prupe.1G017800_v2.0.a1 Prupe.1G162800_v2.0.a1 Prupe.2G312500_v2.0.a1 Prupe.2G312500_v2.0.a1 Prupe.2G312700_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092800_v2.0.a1 Prupe.4G092800_v2.0.a1 Prupe.6G142800_v2.0.a1 Prupe.6G142800_v2.0.a1 Prupe.6G143000_v2.0.a1 Prupe.6G143100_v2.0.a1 Prupe.6G143100_v2.0.a1 Prupe.6G143100_v2.0.a1 Prupe.6G143100_v2.0.a1
pyrus_communis pycom01g23050 pycom01g23070 pycom01g23090 pycom01g23100 pycom01g23110 pycom01g23120 pycom01g23170 pycom07g26410 pycom07g26440 pycom07g26470 pycom07g26480 pycom07g26490 pycom07g26500 pycom07g26510 pycom07g26530 pycom07g26540 pycom07g26570 pycom07g26580 pycom07g26590 pycom08g20940
rosa_chinensis RchiOBHm_Chr1g0324571 RchiOBHm_Chr1g0336181 RchiOBHm_Chr1g0381061 RchiOBHm_Chr1g0381091 RchiOBHm_Chr1g0381121 RchiOBHm_Chr1g0381131 RchiOBHm_Chr1g0381141 RchiOBHm_Chr1g0381161 RchiOBHm_Chr1g0381211 RchiOBHm_Chr2g0110361 RchiOBHm_Chr2g0120231 RchiOBHm_Chr3g0486281 RchiOBHm_Chr3g0486311 RchiOBHm_Chr3g0486401 RchiOBHm_Chr4g0409511 RchiOBHm_Chr4g0409521 RchiOBHm_Chr5g0017271 RchiOBHm_Chr5g0048781 RchiOBHm_Chr5g0048811
rosa_laevigata RLG00000002401 RLG00000008559 RLG00000017798 RLG00000017799 RLG00000023082 RLG00000023088 RLG00000026230 RLG00000026238 RLG00000026240 RLG00000026241 RLG00000026242 RLG00000026243 RLG00000026244 RLG00000026246 RLG00000026247 RLG00000026248 RLG00000029876 RLG00000030189 RLG00000034579
rosa_multiflora Rmu_co8101374.1_g000001 Rmu_co8309149.1_g000001 Rmu_co8450063.1_g000001 Rmu_sc0000441.1_g000094 Rmu_sc0000479.1_g000005 Rmu_sc0000647.1_g000006 Rmu_sc0001393.1_g000031 Rmu_sc0001423.1_g000037 Rmu_sc0001616.1_g000015 Rmu_sc0001857.1_g000011 Rmu_sc0002183.1_g000013 Rmu_sc0002183.1_g000028 Rmu_sc0002183.1_g000030 Rmu_sc0002688.1_g000003 Rmu_sc0002688.1_g000020 Rmu_sc0003801.1_g000003 Rmu_sc0003801.1_g000004 Rmu_sc0004125.1_g000006 Rmu_sc0004494.1_g000005 Rmu_sc0004494.1_g000006 Rmu_sc0004494.1_g000008 Rmu_sc0004494.1_g000009 Rmu_sc0005842.1_g000004 Rmu_sc0006872.1_g000001 Rmu_sc0006872.1_g000005 Rmu_sc0006872.1_g000009 Rmu_sc0006872.1_g000010 Rmu_sc0008053.1_g000026
rosa_roxburghii Rroxscaffold_159G00432960 Rroxscaffold_1G00033060 Rroxscaffold_2G00123150 Rroxscaffold_2G00123170 Rroxscaffold_2G00133500 Rroxscaffold_2G00133550 Rroxscaffold_4G00278650 Rroxscaffold_4G00278750 Rroxscaffold_4G00278790 Rroxscaffold_4G00278800 Rroxscaffold_4G00278810 Rroxscaffold_4G00325390 Rroxscaffold_5G00354010 Rroxscaffold_6G00395890 Rroxscaffold_6G00395900 Rroxscaffold_6G00395920
rosa_rugosa Rorug01G0046900.1 Rorug01G0427000 Rorug01G0427100 Rorug01G0427200 Rorug01G0427300 Rorug01G0427400 Rorug01G0427500 Rorug01G0427500 Rorug01G0427600 Rorug01G0427600 Rorug01G0427700 Rorug01G0427700 Rorug01G0427800 Rorug01G0428700 Rorug02G0166400 Rorug02G0166500 Rorug02G0166500 Rorug02G0166600 Rorug02G0166600 Rorug02G0166700 Rorug02G0167300 Rorug02G0167300 Rorug02G0167300 Rorug03G0224600 Rorug03G0224600 Rorug04G0094400 Rorug05G0241100 Rorug05G0241200
rosa_samantha Rh1BG407700 Rh1BG407900 Rh1BG409000 Rh1CG064700 Rh1CG420900 Rh1CG421000 Rh1CG421200 Rh1CG421300 Rh1CG421800 Rh1CG421900 Rh1CG422000 Rh1CG422200 Rh1CG422300 Rh1CG423600 Rh1DG437600 Rh1DG438900 Rh1DG439000 Rh1DG440300 Rh2AG219100 Rh2CG220700 Rh2DG225300 Rh3AG274400 Rh3AG274600 Rh3CG308900 Rh3DG305800 Rh4AG156400 Rh5AG129000 Rh5AG320900 Rh5CG139600
rosa_wichuraiana Rw1G005300 Rw1G039280 Rw1G039300 Rw1G039310 Rw1G039320 Rw1G039330 Rw1G039340 Rw1G039360 Rw1G039390 Rw2G016910 Rw2G022800 Rw3G024330 Rw3G024340 Rw3G024350 Rw3G024400 Rw4G012810 Rw5G030120 Rw5G030280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 4
Acc65I GGTACC 1 cut(s) 16
AccB1I GGYRCC 1 cut(s) 16
AciI CCGC 2 cut(s) 89, 440
AclWI GGATC 2 cut(s) 89, 586
AcsI RAATTY 2 cut(s) 419, 639
AcuI CTGAAG 1 cut(s) 927
AfaI GTAC 7 cut(s) 18, 62, 732, 749, 832, 1143, 1172
AfiI CCNNNNNNNGG 1 cut(s) 1071
AflIII ACRYGT 1 cut(s) 1138
AgsI TTSAA 3 cut(s) 556, 764, 818
AleI CACNNNNGTG 1 cut(s) 1067
AluBI AGCT 5 cut(s) 293, 478, 512, 692, 740
AluI AGCT 5 cut(s) 293, 478, 512, 692, 740
Alw26I GTCTC 1 cut(s) 211
AlwI GGATC 2 cut(s) 89, 586
AoxI GGCC 2 cut(s) 565, 921
ApeKI GCWGC 3 cut(s) 368, 437, 580
ApoI RAATTY 2 cut(s) 419, 639
AsiSI GCGATCGC 1 cut(s) 308
Asp718I GGTACC 1 cut(s) 16
AspLEI GCGC 2 cut(s) 85, 489
AspS9I GGNCC 1 cut(s) 922
AsuII TTCGAA 1 cut(s) 425
AsuNHI GCTAGC 1 cut(s) 478
BanI GGYRCC 1 cut(s) 16
BarI GAAGNNNNNNTAC 2 cut(s) 986, 1018
BbsI GAAGAC 2 cut(s) 276, 541
BbvI GCAGC 3 cut(s) 355, 449, 592
BcgI CGANNNNNNTGC 2 cut(s) 1033, 1067
BcoDI GTCTC 1 cut(s) 211
BfaI CTAG 2 cut(s) 479, 539
BfmI CTRYAG 1 cut(s) 288
BfoI RGCGCY 1 cut(s) 490
BfuAI ACCTGC 1 cut(s) 4
BglII AGATCT 1 cut(s) 630
BisI GCNGC 3 cut(s) 369, 438, 581
BlsI GCNGC 3 cut(s) 370, 439, 582
BmgT120I GGNCC 1 cut(s) 922
BmiI GGNNCC 2 cut(s) 18, 924
BmsI GCATC 4 cut(s) 51, 106, 416, 850
BmtI GCTAGC 1 cut(s) 482
BpiI GAAGAC 2 cut(s) 276, 541
Bpu10I CCTNAGC 1 cut(s) 918
Bpu14I TTCGAA 1 cut(s) 425
Bsa29I ATCGAT 1 cut(s) 243
BsaAI YACGTR 1 cut(s) 1025
BsaBI GATNNNNATC 1 cut(s) 41
BsaJI CCNNGG 1 cut(s) 101
Bsc4I CCNNNNNNNGG 1 cut(s) 1071
Bse1I ACTGG 2 cut(s) 408, 679
Bse8I GATNNNNATC 1 cut(s) 41
BseCI ATCGAT 1 cut(s) 243
BseDI CCNNGG 1 cut(s) 101
BseJI GATNNNNATC 1 cut(s) 41
BseLI CCNNNNNNNGG 1 cut(s) 1071
BseMII CTCAG 2 cut(s) 668, 676
BseNI ACTGG 2 cut(s) 408, 679
BseXI GCAGC 3 cut(s) 355, 449, 592
BseYI CCCAGC 1 cut(s) 470
Bsh1285I CGRYCG 1 cut(s) 308
BshFI GGCC 2 cut(s) 567, 923
BshNI GGYRCC 1 cut(s) 16
BshVI ATCGAT 1 cut(s) 243
BsiEI CGRYCG 1 cut(s) 308
BslFI GGGAC 1 cut(s) 14
BslI CCNNNNNNNGG 1 cut(s) 1071
BsmAI GTCTC 1 cut(s) 211
BsmFI GGGAC 1 cut(s) 14
BsmI GAATGC 1 cut(s) 919
BsnI GGCC 2 cut(s) 567, 923
Bsp119I TTCGAA 1 cut(s) 425
Bsp1407I TGTACA 1 cut(s) 1141
Bsp143I GATC 5 cut(s) 94, 109, 305, 591, 630
BspACI CCGC 2 cut(s) 89, 440
BspANI GGCC 2 cut(s) 567, 923
BspCNI CTCAG 2 cut(s) 667, 675
BspDI ATCGAT 1 cut(s) 243
BspLI GGNNCC 2 cut(s) 18, 924
BspMI ACCTGC 1 cut(s) 4
BspOI GCTAGC 1 cut(s) 482
BspPI GGATC 2 cut(s) 89, 586
BspT104I TTCGAA 1 cut(s) 425
BspT107I GGYRCC 1 cut(s) 16
BsrGI TGTACA 1 cut(s) 1141
BsrI ACTGG 2 cut(s) 408, 679
BssECI CCNNGG 1 cut(s) 101
BssMI GATC 5 cut(s) 94, 109, 305, 591, 630
BssT1I CCWWGG 1 cut(s) 101
Bst4CI ACNGT 1 cut(s) 1175
Bst6I CTCTTC 1 cut(s) 903
BstAPI GCANNNNNTGC 1 cut(s) 869
BstAUI TGTACA 1 cut(s) 1141
BstBAI YACGTR 1 cut(s) 1025
BstBI TTCGAA 1 cut(s) 425
BstC8I GCNNGC 2 cut(s) 480, 1112
BstDEI CTNAG 4 cut(s) 654, 662, 918, 1031
BstH2I RGCGCY 1 cut(s) 490
BstHHI GCGC 2 cut(s) 85, 489
BstKTI GATC 5 cut(s) 97, 112, 308, 594, 633
BstMAI GTCTC 1 cut(s) 211
BstMBI GATC 5 cut(s) 94, 109, 305, 591, 630
BstMCI CGRYCG 1 cut(s) 308
BstMWI GCNNNNNNNGC 6 cut(s) 125, 365, 484, 493, 532, 869
BstNSI RCATGY 3 cut(s) 195, 795, 1142
BstSFI CTRYAG 1 cut(s) 288
BstSNI TACGTA 1 cut(s) 1025
BstV1I GCAGC 3 cut(s) 355, 449, 592
BstV2I GAAGAC 2 cut(s) 276, 541
BstX2I RGATCY 3 cut(s) 94, 591, 630
BstYI RGATCY 3 cut(s) 94, 591, 630
Bsu15I ATCGAT 1 cut(s) 243
BsuRI GGCC 2 cut(s) 567, 923
BsuTUI ATCGAT 1 cut(s) 243
BveI ACCTGC 1 cut(s) 4
Cac8I GCNNGC 2 cut(s) 480, 1112
CfoI GCGC 2 cut(s) 85, 489
Cfr13I GGNCC 1 cut(s) 922
ClaI ATCGAT 1 cut(s) 243
Csp6I GTAC 7 cut(s) 17, 61, 731, 748, 831, 1142, 1171
CviAII CATG 8 cut(s) 192, 347, 483, 523, 569, 728, 792, 1139
CviQI GTAC 7 cut(s) 17, 61, 731, 748, 831, 1142, 1171
DdeI CTNAG 4 cut(s) 654, 662, 918, 1031
DpnI GATC 5 cut(s) 96, 111, 307, 593, 632
DpnII GATC 5 cut(s) 94, 109, 305, 591, 630
Eam1104I CTCTTC 1 cut(s) 903
EarI CTCTTC 1 cut(s) 903
Eco105I TACGTA 1 cut(s) 1025
Eco130I CCWWGG 1 cut(s) 101
Eco57I CTGAAG 1 cut(s) 927
EcoO109I RGGNCCY 1 cut(s) 922
EcoT14I CCWWGG 1 cut(s) 101
EcoT22I ATGCAT 1 cut(s) 574
ErhI CCWWGG 1 cut(s) 101
FaeI CATG 8 cut(s) 195, 350, 486, 526, 572, 731, 795, 1142
FalI AAGNNNNNCTT 2 cut(s) 135, 167
FaqI GGGAC 1 cut(s) 14
FatI CATG 8 cut(s) 191, 346, 482, 522, 568, 727, 791, 1138
Fnu4HI GCNGC 3 cut(s) 369, 438, 581
Fsp4HI GCNGC 3 cut(s) 369, 438, 581
FspBI CTAG 2 cut(s) 479, 539
GlaI GCGC 2 cut(s) 84, 488
GluI GCNGC 3 cut(s) 369, 438, 581
GsaI CCCAGC 1 cut(s) 474
HaeII RGCGCY 1 cut(s) 490
HaeIII GGCC 2 cut(s) 567, 923
HhaI GCGC 2 cut(s) 85, 489
Hin1II CATG 8 cut(s) 195, 350, 486, 526, 572, 731, 795, 1142
Hin6I GCGC 2 cut(s) 83, 487
HinP1I GCGC 2 cut(s) 83, 487
HinfI GANTC 2 cut(s) 950, 1157
Hpy166II GTNNAC 1 cut(s) 850
Hpy188I TCNGA 5 cut(s) 400, 412, 657, 665, 989
Hpy188III TCNNGA 5 cut(s) 113, 169, 595, 947, 1052
Hpy8I GTNNAC 1 cut(s) 850
HpyAV CCTTC 3 cut(s) 137, 242, 421
HpyCH4III ACNGT 1 cut(s) 1175
HpyCH4IV ACGT 1 cut(s) 1024
HpyF10VI GCNNNNNNNGC 6 cut(s) 125, 365, 484, 493, 532, 869
HpyF3I CTNAG 4 cut(s) 654, 662, 918, 1031
HpySE526I ACGT 1 cut(s) 1024
Hsp92II CATG 8 cut(s) 195, 350, 486, 526, 572, 731, 795, 1142
HspAI GCGC 2 cut(s) 83, 487
KpnI GGTACC 1 cut(s) 20
Kzo9I GATC 5 cut(s) 94, 109, 305, 591, 630
LmnI GCTCC 1 cut(s) 737
Lsp1109I GCAGC 3 cut(s) 355, 449, 592
LweI GCATC 4 cut(s) 51, 106, 416, 850
MaeI CTAG 2 cut(s) 479, 539
MaeII ACGT 1 cut(s) 1024
MaeIII GTNAC 1 cut(s) 319
MalI GATC 5 cut(s) 96, 111, 307, 593, 632
MboI GATC 5 cut(s) 94, 109, 305, 591, 630
MboII GAAGA 7 cut(s) 217, 281, 541, 544, 599, 830, 920
MflI RGATCY 3 cut(s) 94, 591, 630
MnlI CCTC 5 cut(s) 73, 276, 557, 838, 965
Mph1103I ATGCAT 1 cut(s) 574
MseI TTAA 4 cut(s) 57, 363, 699, 882
MslI CAYNNNNRTG 1 cut(s) 1067
MspA1I CMGCKG 1 cut(s) 440
Mva1269I GAATGC 1 cut(s) 919
MwoI GCNNNNNNNGC 6 cut(s) 125, 365, 484, 493, 532, 869
NdeII GATC 5 cut(s) 94, 109, 305, 591, 630
NheI GCTAGC 1 cut(s) 478
NlaIII CATG 8 cut(s) 195, 350, 486, 526, 572, 731, 795, 1142
NlaIV GGNNCC 2 cut(s) 18, 924
NmuCI GTSAC 1 cut(s) 319
NsiI ATGCAT 1 cut(s) 574
NspI RCATGY 3 cut(s) 195, 795, 1142
NspV TTCGAA 1 cut(s) 425
OliI CACNNNNGTG 1 cut(s) 1067
PciI ACATGT 1 cut(s) 1138
PctI GAATGC 1 cut(s) 919
PfeI GAWTC 2 cut(s) 950, 1157
PkrI GCNGC 3 cut(s) 370, 439, 582
Ple19I CGATCG 1 cut(s) 308
Ppu21I YACGTR 1 cut(s) 1025
PscI ACATGT 1 cut(s) 1138
PspFI CCCAGC 1 cut(s) 470
PspN4I GGNNCC 2 cut(s) 18, 924
PspPI GGNCC 1 cut(s) 922
PsuI RGATCY 3 cut(s) 94, 591, 630
PvuI CGATCG 1 cut(s) 308
RgaI GCGATCGC 1 cut(s) 308
RsaI GTAC 7 cut(s) 18, 62, 732, 749, 832, 1143, 1172
RsaNI GTAC 7 cut(s) 17, 61, 731, 748, 831, 1142, 1171
RseI CAYNNNNRTG 1 cut(s) 1067
SaqAI TTAA 4 cut(s) 57, 363, 699, 882
SatI GCNGC 3 cut(s) 369, 438, 581
Sau3AI GATC 5 cut(s) 94, 109, 305, 591, 630
Sau96I GGNCC 1 cut(s) 922
SfaAI GCGATCGC 1 cut(s) 308
SfaNI GCATC 4 cut(s) 51, 106, 416, 850
SfcI CTRYAG 1 cut(s) 288
SfuI TTCGAA 1 cut(s) 425
SgfI GCGATCGC 1 cut(s) 308
SmiMI CAYNNNNRTG 1 cut(s) 1067
SnaBI TACGTA 1 cut(s) 1025
SsiI CCGC 2 cut(s) 89, 440
SspI AATATT 1 cut(s) 942
SspMI CTAG 2 cut(s) 479, 539
StyI CCWWGG 1 cut(s) 101
TaaI ACNGT 1 cut(s) 1175
TaiI ACGT 1 cut(s) 1027
TaqI TCGA 6 cut(s) 69, 112, 243, 425, 637, 1053
TatI WGTACW 3 cut(s) 60, 730, 1141
TfiI GAWTC 2 cut(s) 950, 1157
Tru1I TTAA 4 cut(s) 57, 363, 699, 882
Tru9I TTAA 4 cut(s) 57, 363, 699, 882
TseFI GTSAC 1 cut(s) 319
TseI GCWGC 3 cut(s) 368, 437, 580
Tsp45I GTSAC 1 cut(s) 319
TspDTI ATGAA 3 cut(s) 153, 261, 1170
XapI RAATTY 2 cut(s) 419, 639
XceI RCATGY 3 cut(s) 195, 795, 1142
XcmI CCANNNNNNNNNTGG 1 cut(s) 99
XspI CTAG 2 cut(s) 479, 539
Zsp2I ATGCAT 1 cut(s) 574
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.