Rh1CG421300

Cyclic nucleotide-gated ion channel 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0000119
Physical Location & Seq
Reverse (-)
68058740 .. 68097142
38403 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG421300.1

Sequence Viewer

Length: 999 bp
ATGATTAACTCGATGGATGAAGCCCCAGAAGTAAGTCTCACAGGTTACACTACTCATTATGGATATGGTCGGAAACCATCAGAAGTGAAAAAAACGAAAAGGAGATTTAGTTCGTGGTGGACTAGCTTTTCGGTTCGAGGTACATGGTGGGATTCGATATTTGTAGCCTCGTGTGTTATCGCAGTCTCACTGGATCCACTGTTCTTTTACATTCCTATCATCGATAGGGAAAAAAAGTGCCTTCAAGCAGACAGACAATTGAGGACTGTAACTCTCATTTTGCGATCAGTGATGGATATCACTTTTATAGTGCATATTATCTATCAGATTCGTGAAGCTATGATCGCTGCAGCCCCCGATAAGATGGCTAACCAGGAAGAACCGAAGTCTTCCGATTTATTTAAAGGACCCTTCTTTAAACGTGTCGGTGCAATAGTTGACAATTTGTCATGGCGCTCTCTCATAACTGACATTCTAGCTGTTCTTCCTATCCCACAGGTGCTACTACTTGTTGTCTTCTTTAAAATGAGAGGCTCTGGATATTTGAACCACAGAAAGATTTTGAATTTCTTTCTTCTCCTTCAATATTTGGCAAGAATTTATCGTATTCACCTATCTTCCAAAAAACTTACAAAGAGTCATGGAATATGGATTAAAGGACTATTCAATTTATTTCTCTATATCTTTGCAAGTCACGTACTTGGAGCCTTTTGGTATTTCTTTTCGATTCAACGAGAGACATCATGTTGGCATCGAAGTTGCGGTCCCATCTGTATAAGTAACTTTTATTGTGATGAGTACAAACATTCAGACGATATGGTGACGCTTATAACATCTCTGAATGCATCGTGCCCTATAAATACTACTGGACCATTTGATTTTGGAATATTTCTTGATTCTCTAAAGTCCAATAACCCGGCATCAATCAACTTTCCACAAAAGTTGTGTTACTCTTTTTGGTGGGGTCTACGAAATTTGAGGTTTGCATATATATGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
Ion_trans

Protein Analysis

332

Amino Acids

38.52

Weight (kDa)

9.39

Isoelectric Point (pI)

36.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ion_trans PF00520 48 - 325 3.3e-07 Ion transport protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000119)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g10570 FvH4_4g36390 FvH4_7g31840 FvH4_7g31840 FvH4_7g31850 FvH4_7g31850 FvH4_7g31861 FvH4_7g31861 FvH4_7g31950
malus_domestica MD01G1054600.v1.1 MD01G1221100.v1.1 MD01G1221400.v1.1 MD01G1221600.v1.1 MD01G1221800.v1.1 MD01G1222000.v1.1 MD01G1222100.v1.1 MD07G1140800.v1.1 MD07G1291200.v1.1 MD07G1291400.v1.1 MD07G1291500.v1.1 MD07G1291700.v1.1 MD07G1292500.v1.1 MD07G1292700.v1.1 MD07G1292800.v1.1 MD07G1292900.v1.1 MD08G1241600.v1.1 MD11G1083800.v1.1 MD15G1431600.v1.1
prunus_persica Prupe.1G017800_v2.0.a1 Prupe.1G017800_v2.0.a1 Prupe.1G162800_v2.0.a1 Prupe.2G312500_v2.0.a1 Prupe.2G312500_v2.0.a1 Prupe.2G312700_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092800_v2.0.a1 Prupe.4G092800_v2.0.a1 Prupe.6G142800_v2.0.a1 Prupe.6G142800_v2.0.a1 Prupe.6G143000_v2.0.a1 Prupe.6G143100_v2.0.a1 Prupe.6G143100_v2.0.a1 Prupe.6G143100_v2.0.a1 Prupe.6G143100_v2.0.a1
pyrus_communis pycom01g23050 pycom01g23070 pycom01g23090 pycom01g23100 pycom01g23110 pycom01g23120 pycom01g23170 pycom07g26410 pycom07g26440 pycom07g26470 pycom07g26480 pycom07g26490 pycom07g26500 pycom07g26510 pycom07g26530 pycom07g26540 pycom07g26570 pycom07g26580 pycom07g26590 pycom08g20940
rosa_chinensis RchiOBHm_Chr1g0324571 RchiOBHm_Chr1g0336181 RchiOBHm_Chr1g0381061 RchiOBHm_Chr1g0381091 RchiOBHm_Chr1g0381121 RchiOBHm_Chr1g0381131 RchiOBHm_Chr1g0381141 RchiOBHm_Chr1g0381161 RchiOBHm_Chr1g0381211 RchiOBHm_Chr2g0110361 RchiOBHm_Chr2g0120231 RchiOBHm_Chr3g0486281 RchiOBHm_Chr3g0486311 RchiOBHm_Chr3g0486401 RchiOBHm_Chr4g0409511 RchiOBHm_Chr4g0409521 RchiOBHm_Chr5g0017271 RchiOBHm_Chr5g0048781 RchiOBHm_Chr5g0048811
rosa_laevigata RLG00000002401 RLG00000008559 RLG00000017798 RLG00000017799 RLG00000023082 RLG00000023088 RLG00000026230 RLG00000026238 RLG00000026240 RLG00000026241 RLG00000026242 RLG00000026243 RLG00000026244 RLG00000026246 RLG00000026247 RLG00000026248 RLG00000029876 RLG00000030189 RLG00000034579
rosa_multiflora Rmu_co8101374.1_g000001 Rmu_co8309149.1_g000001 Rmu_co8450063.1_g000001 Rmu_sc0000441.1_g000094 Rmu_sc0000479.1_g000005 Rmu_sc0000647.1_g000006 Rmu_sc0001393.1_g000031 Rmu_sc0001423.1_g000037 Rmu_sc0001616.1_g000015 Rmu_sc0001857.1_g000011 Rmu_sc0002183.1_g000013 Rmu_sc0002183.1_g000028 Rmu_sc0002183.1_g000030 Rmu_sc0002688.1_g000003 Rmu_sc0002688.1_g000020 Rmu_sc0003801.1_g000003 Rmu_sc0003801.1_g000004 Rmu_sc0004125.1_g000006 Rmu_sc0004494.1_g000005 Rmu_sc0004494.1_g000006 Rmu_sc0004494.1_g000008 Rmu_sc0004494.1_g000009 Rmu_sc0005842.1_g000004 Rmu_sc0006872.1_g000001 Rmu_sc0006872.1_g000005 Rmu_sc0006872.1_g000009 Rmu_sc0006872.1_g000010 Rmu_sc0008053.1_g000026
rosa_roxburghii Rroxscaffold_159G00432960 Rroxscaffold_1G00033060 Rroxscaffold_2G00123150 Rroxscaffold_2G00123170 Rroxscaffold_2G00133500 Rroxscaffold_2G00133550 Rroxscaffold_4G00278650 Rroxscaffold_4G00278750 Rroxscaffold_4G00278790 Rroxscaffold_4G00278800 Rroxscaffold_4G00278810 Rroxscaffold_4G00325390 Rroxscaffold_5G00354010 Rroxscaffold_6G00395890 Rroxscaffold_6G00395900 Rroxscaffold_6G00395920
rosa_rugosa Rorug01G0046900.1 Rorug01G0427000 Rorug01G0427100 Rorug01G0427200 Rorug01G0427300 Rorug01G0427400 Rorug01G0427500 Rorug01G0427500 Rorug01G0427600 Rorug01G0427600 Rorug01G0427700 Rorug01G0427700 Rorug01G0427800 Rorug01G0428700 Rorug02G0166400 Rorug02G0166500 Rorug02G0166500 Rorug02G0166600 Rorug02G0166600 Rorug02G0166700 Rorug02G0167300 Rorug02G0167300 Rorug02G0167300 Rorug03G0224600 Rorug03G0224600 Rorug04G0094400 Rorug05G0241100 Rorug05G0241200
rosa_samantha Rh1BG407700 Rh1BG407900 Rh1BG409000 Rh1CG064700 Rh1CG420900 Rh1CG421000 Rh1CG421200 Rh1CG421300 Rh1CG421800 Rh1CG421900 Rh1CG422000 Rh1CG422200 Rh1CG422300 Rh1CG423600 Rh1DG437600 Rh1DG438900 Rh1DG439000 Rh1DG440300 Rh2AG219100 Rh2CG220700 Rh2DG225300 Rh3AG274400 Rh3AG274600 Rh3CG308900 Rh3DG305800 Rh4AG156400 Rh5AG129000 Rh5AG320900 Rh5CG139600
rosa_wichuraiana Rw1G005300 Rw1G039280 Rw1G039300 Rw1G039310 Rw1G039320 Rw1G039330 Rw1G039340 Rw1G039360 Rw1G039390 Rw2G016910 Rw2G022800 Rw3G024330 Rw3G024340 Rw3G024350 Rw3G024400 Rw4G012810 Rw5G030120 Rw5G030280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 830
AccI GTMKAC 1 cut(s) 967
AciI CCGC 1 cut(s) 762
AclWI GGATC 2 cut(s) 188, 201
AcsI RAATTY 3 cut(s) 565, 597, 973
AfaI GTAC 3 cut(s) 142, 699, 800
AflIII ACRYGT 1 cut(s) 421
AgsI TTSAA 6 cut(s) 245, 547, 565, 584, 667, 731
AhdI GACNNNNNGTC 1 cut(s) 445
AjnI CCWGG 1 cut(s) 372
AloI GAACNNNNNNTCC 4 cut(s) 94, 126, 185, 217
AluBI AGCT 3 cut(s) 126, 338, 479
AluI AGCT 3 cut(s) 126, 338, 479
Alw26I GTCTC 3 cut(s) 41, 190, 731
AlwI GGATC 2 cut(s) 188, 201
ApeKI GCWGC 2 cut(s) 347, 350
ApoI RAATTY 3 cut(s) 565, 597, 973
ArsI GACNNNNNNTTYG 4 cut(s) 112, 144, 748, 780
AspLEI GCGC 1 cut(s) 456
AspS9I GGNCC 3 cut(s) 407, 764, 869
AsuC2I CCSGG 1 cut(s) 917
AsuHPI GGTGA 2 cut(s) 602, 832
AvaII GGWCC 3 cut(s) 407, 764, 869
BaeGI GKGCMC 1 cut(s) 854
BamHI GGATCC 1 cut(s) 193
BauI CACGAG 1 cut(s) 169
BbsI GAAGAC 2 cut(s) 381, 508
BbvI GCAGC 2 cut(s) 334, 362
BccI CCATC 5 cut(s) 7, 85, 286, 358, 776
BciT130I CCWGG 1 cut(s) 374
BcnI CCSGG 1 cut(s) 917
BcoDI GTCTC 3 cut(s) 41, 190, 731
BfaI CTAG 2 cut(s) 123, 476
BfmI CTRYAG 1 cut(s) 348
BfoI RGCGCY 1 cut(s) 457
BisI GCNGC 2 cut(s) 348, 351
BlsI GCNGC 2 cut(s) 349, 352
Bme1390I CCNGG 2 cut(s) 374, 917
Bme18I GGWCC 3 cut(s) 407, 764, 869
BmeRI GACNNNNNGTC 1 cut(s) 445
BmgT120I GGNCC 3 cut(s) 407, 764, 869
BmiI GGNNCC 4 cut(s) 195, 409, 706, 766
BmrFI CCNGG 2 cut(s) 374, 917
BmsI GCATC 3 cut(s) 760, 854, 929
BpiI GAAGAC 2 cut(s) 381, 508
BpuMI CCSGG 1 cut(s) 917
Bsa29I ATCGAT 1 cut(s) 222
BsaAI YACGTR 1 cut(s) 697
BsaBI GATNNNNATC 1 cut(s) 296
BsaXI ACNNNNNCTCC 2 cut(s) 94, 124
Bse1I ACTGG 2 cut(s) 195, 871
Bse8I GATNNNNATC 1 cut(s) 296
BseBI CCWGG 1 cut(s) 374
BseCI ATCGAT 1 cut(s) 222
BseGI GGATG 1 cut(s) 22
BseJI GATNNNNATC 1 cut(s) 296
BseNI ACTGG 2 cut(s) 195, 871
BseSI GKGCMC 1 cut(s) 854
BseXI GCAGC 2 cut(s) 334, 362
BshVI ATCGAT 1 cut(s) 222
BsiSI CCGG 1 cut(s) 917
BslFI GGGAC 1 cut(s) 750
BsmAI GTCTC 3 cut(s) 41, 190, 731
BsmFI GGGAC 1 cut(s) 750
BsmI GAATGC 1 cut(s) 847
Bsp1286I GDGCHC 1 cut(s) 854
Bsp143I GATC 3 cut(s) 193, 284, 342
BspACI CCGC 1 cut(s) 762
BspDI ATCGAT 1 cut(s) 222
BspLI GGNNCC 4 cut(s) 195, 409, 706, 766
BspMAI CTGCAG 1 cut(s) 352
BspPI GGATC 2 cut(s) 188, 201
BsrI ACTGG 2 cut(s) 195, 871
BssMI GATC 3 cut(s) 193, 284, 342
BssSI CACGAG 1 cut(s) 169
Bst2BI CACGAG 1 cut(s) 169
Bst2UI CCWGG 1 cut(s) 374
Bst4CI ACNGT 2 cut(s) 201, 268
BstBAI YACGTR 1 cut(s) 697
BstF5I GGATG 1 cut(s) 22
BstH2I RGCGCY 1 cut(s) 457
BstHHI GCGC 1 cut(s) 456
BstKTI GATC 3 cut(s) 196, 287, 345
BstMAI GTCTC 3 cut(s) 41, 190, 731
BstMBI GATC 3 cut(s) 193, 284, 342
BstMWI GCNNNNNNNGC 1 cut(s) 344
BstNI CCWGG 1 cut(s) 374
BstSCI CCNGG 2 cut(s) 372, 915
BstSFI CTRYAG 1 cut(s) 348
BstSLI GKGCMC 1 cut(s) 854
BstV1I GCAGC 2 cut(s) 334, 362
BstV2I GAAGAC 2 cut(s) 381, 508
BstX2I RGATCY 1 cut(s) 193
BstYI RGATCY 1 cut(s) 193
Bsu15I ATCGAT 1 cut(s) 222
BsuTUI ATCGAT 1 cut(s) 222
BtsCI GGATG 1 cut(s) 22
BtsIMutI CAGTG 3 cut(s) 188, 197, 294
CfoI GCGC 1 cut(s) 456
Cfr13I GGNCC 3 cut(s) 407, 764, 869
ClaI ATCGAT 1 cut(s) 222
CseI GACGC 1 cut(s) 832
Csp6I GTAC 3 cut(s) 141, 698, 799
CspCI CAANNNNNGTGG 2 cut(s) 924, 959
CviAII CATG 4 cut(s) 144, 450, 641, 744
CviJI RGCY 9 cut(s) 23, 126, 167, 338, 353, 368, 479, 534, 707
CviKI_1 RGCY 9 cut(s) 23, 126, 167, 338, 353, 368, 479, 534, 707
CviQI GTAC 3 cut(s) 141, 698, 799
DpnI GATC 3 cut(s) 195, 286, 344
DpnII GATC 3 cut(s) 193, 284, 342
DraI TTTAAA 3 cut(s) 403, 418, 523
DriI GACNNNNNGTC 1 cut(s) 445
Eam1105I GACNNNNNGTC 1 cut(s) 445
Eco32I GATATC 1 cut(s) 298
Eco47I GGWCC 3 cut(s) 407, 764, 869
EcoO109I RGGNCCY 1 cut(s) 407
EcoRII CCWGG 1 cut(s) 372
EcoRV GATATC 1 cut(s) 298
EcoT22I ATGCAT 1 cut(s) 847
FaeI CATG 4 cut(s) 147, 453, 644, 747
FaqI GGGAC 1 cut(s) 750
FatI CATG 4 cut(s) 143, 449, 640, 743
FblI GTMKAC 1 cut(s) 967
Fnu4HI GCNGC 2 cut(s) 348, 351
FokI GGATG 1 cut(s) 29
Fsp4HI GCNGC 2 cut(s) 348, 351
FspBI CTAG 2 cut(s) 123, 476
GlaI GCGC 1 cut(s) 455
GluI GCNGC 2 cut(s) 348, 351
HaeII RGCGCY 1 cut(s) 457
HapII CCGG 1 cut(s) 917
HgaI GACGC 1 cut(s) 832
HhaI GCGC 1 cut(s) 456
Hin1II CATG 4 cut(s) 147, 453, 644, 747
Hin6I GCGC 1 cut(s) 454
HinP1I GCGC 1 cut(s) 454
HincII GTYRAC 1 cut(s) 439
HindII GTYRAC 1 cut(s) 439
HinfI GANTC 5 cut(s) 152, 328, 637, 727, 896
HpaII CCGG 1 cut(s) 917
HphI GGTGA 2 cut(s) 602, 832
Hpy166II GTNNAC 3 cut(s) 120, 439, 968
Hpy188I TCNGA 6 cut(s) 72, 82, 327, 394, 811, 840
Hpy188III TCNNGA 3 cut(s) 332, 537, 893
Hpy8I GTNNAC 3 cut(s) 120, 439, 968
HpyAV CCTTC 3 cut(s) 251, 421, 590
HpyCH4III ACNGT 2 cut(s) 201, 268
HpyCH4IV ACGT 2 cut(s) 421, 696
HpyCH4V TGCA 6 cut(s) 313, 350, 431, 689, 845, 986
HpyF10VI GCNNNNNNNGC 1 cut(s) 344
HpySE526I ACGT 2 cut(s) 421, 696
Hsp92II CATG 4 cut(s) 147, 453, 644, 747
HspAI GCGC 1 cut(s) 454
Kzo9I GATC 3 cut(s) 193, 284, 342
LmnI GCTCC 1 cut(s) 704
LpnPI CCDG 9 cut(s) 27, 39, 176, 359, 386, 482, 522, 852, 930
Lsp1109I GCAGC 2 cut(s) 334, 362
LweI GCATC 3 cut(s) 760, 854, 929
MaeI CTAG 2 cut(s) 123, 476
MaeII ACGT 2 cut(s) 421, 696
MaeIII GTNAC 6 cut(s) 44, 268, 692, 779, 820, 947
MalI GATC 3 cut(s) 195, 286, 344
MboI GATC 3 cut(s) 193, 284, 342
MboII GAAGA 6 cut(s) 381, 389, 476, 508, 566, 609
MfeI CAATTG 1 cut(s) 257
MflI RGATCY 1 cut(s) 193
MhlI GDGCHC 1 cut(s) 854
MluCI AATT 6 cut(s) 257, 442, 565, 597, 667, 973
MlyI GAGTC 1 cut(s) 646
MmeI TCCRAC 1 cut(s) 50
MnlI CCTC 5 cut(s) 131, 178, 255, 524, 972
Mph1103I ATGCAT 1 cut(s) 847
MseI TTAA 5 cut(s) 6, 402, 417, 522, 654
MslI CAYNNNNRTG 1 cut(s) 991
MspI CCGG 1 cut(s) 917
MspR9I CCNGG 2 cut(s) 374, 917
MunI CAATTG 1 cut(s) 257
Mva1269I GAATGC 1 cut(s) 847
MvaI CCWGG 1 cut(s) 374
MwoI GCNNNNNNNGC 1 cut(s) 344
NciI CCSGG 1 cut(s) 917
NdeII GATC 3 cut(s) 193, 284, 342
NlaIII CATG 4 cut(s) 147, 453, 644, 747
NlaIV GGNNCC 4 cut(s) 195, 409, 706, 766
NmuCI GTSAC 2 cut(s) 692, 820
NsiI ATGCAT 1 cut(s) 847
PctI GAATGC 1 cut(s) 847
PfeI GAWTC 4 cut(s) 152, 328, 727, 896
PkrI GCNGC 2 cut(s) 349, 352
PleI GAGTC 1 cut(s) 645
PpsI GAGTC 1 cut(s) 645
Ppu21I YACGTR 1 cut(s) 697
PpuMI RGGWCCY 1 cut(s) 407
PsiI TTATAA 1 cut(s) 830
Psp5II RGGWCCY 1 cut(s) 407
Psp6I CCWGG 1 cut(s) 372
PspGI CCWGG 1 cut(s) 372
PspN4I GGNNCC 4 cut(s) 195, 409, 706, 766
PspPI GGNCC 3 cut(s) 407, 764, 869
PspPPI RGGWCCY 1 cut(s) 407
PstI CTGCAG 1 cut(s) 352
PsuI RGATCY 1 cut(s) 193
RsaI GTAC 3 cut(s) 142, 699, 800
RsaNI GTAC 3 cut(s) 141, 698, 799
RseI CAYNNNNRTG 1 cut(s) 991
SaqAI TTAA 5 cut(s) 6, 402, 417, 522, 654
SatI GCNGC 2 cut(s) 348, 351
Sau3AI GATC 3 cut(s) 193, 284, 342
Sau96I GGNCC 3 cut(s) 407, 764, 869
SchI GAGTC 1 cut(s) 646
ScrFI CCNGG 2 cut(s) 374, 917
SduI GDGCHC 1 cut(s) 854
SfaNI GCATC 3 cut(s) 760, 854, 929
SfcI CTRYAG 1 cut(s) 348
SinI GGWCC 3 cut(s) 407, 764, 869
SmiMI CAYNNNNRTG 1 cut(s) 991
Sse9I AATT 6 cut(s) 257, 442, 565, 597, 667, 973
SsiI CCGC 1 cut(s) 762
SspI AATATT 2 cut(s) 587, 888
SspMI CTAG 2 cut(s) 123, 476
StyD4I CCNGG 2 cut(s) 372, 915
TaaI ACNGT 2 cut(s) 201, 268
TaiI ACGT 2 cut(s) 424, 699
TaqI TCGA 6 cut(s) 11, 136, 155, 222, 725, 754
TasI AATT 6 cut(s) 257, 442, 565, 597, 667, 973
TatI WGTACW 1 cut(s) 798
TfiI GAWTC 4 cut(s) 152, 328, 727, 896
Tru1I TTAA 5 cut(s) 6, 402, 417, 522, 654
Tru9I TTAA 5 cut(s) 6, 402, 417, 522, 654
TscAI CASTG 3 cut(s) 195, 204, 294
TseFI GTSAC 2 cut(s) 692, 820
TseI GCWGC 2 cut(s) 347, 350
Tsp45I GTSAC 2 cut(s) 692, 820
TspDTI ATGAA 1 cut(s) 33
TspRI CASTG 3 cut(s) 195, 204, 294
VpaK11BI GGWCC 3 cut(s) 407, 764, 869
XapI RAATTY 3 cut(s) 565, 597, 973
XmiI GTMKAC 1 cut(s) 967
XspI CTAG 2 cut(s) 123, 476
Zsp2I ATGCAT 1 cut(s) 847
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.