Rorug02G0167300

Cyclic nucleotide-gated ion channel 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000119
Physical Location & Seq
Forward (+)
15004224 .. 15011875
7652 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0167300.1

Sequence Viewer

Length: 2457 bp
ATGGTATTAGTCAGAGGAGAAGCTTTAGAAGCATCATTTGTGGCACTGTATGATGCCATTAAGGAAGTAAATGAACATCATTTGATGTTTAAAGCCCTCCTTGGAGATATCAGATCTACACTAGATTCTATAGAACCACTGATCAAAGAGACAGCAAAGGATGACAAGGAGTTGGATTTTCCAGAGGATGAACTTCATAATTTTGGAGTACAAATGGAAGAGGGGATTGAGCTAATTCGTAAGTGCTCTAAGGTTGGTCCATTGAGTACTTACAAAAAATATAAATACACAAACAAACTAGGTGAATTGGGAAAATCTTTGCAAAGACTGTTGGATATACTGAAAGTGCATAGCATAAGAGATGTGAAGAAAACCTTGGTTACCGTAAAGAATATTGAGACTAAGGTCCAGCAAATTGATGTGAATATTGGGATACAAAATAATTCATCTGAAAGTATTGAAGGTTCGTGTGCAATACCTGAACCTCCACCACTTGTAGTTGGATTGGATGGGCCTTTGAAGGAATTGAAGATGAAGCTTTTCAGTGATGAGATATCAATGCTTGTGCTAACTGCTCCTGGAGGATGTGGGAAAACTACATTGGCAACGAAGTTTTGTCAAGATGGAGACGTCAGAGATAAATTCAAGAACAATATCTTCTTTGTAACTGTTTCCAAGAAGCCCAACTTAAACCTTATTGTAGAAGAGCTATATCAACGCAAGGGTTCCCAGGTACCCGATTTCCAACATGAAGTAATTGCACTTAATTGGCTGCACGAATTTCTAAACCAAATGGGACAAGATCCTTTACTATTGGTTTTGGATGATGTTTGGTCTGAATCTGAATTTCTTCTTGAGAAGTTTGATGAATGGAGAATGTCTAATTGCAAGATCTTGGTGACATCAAGGTTTGCATTTCCAAGATTTGGTTCTCCATATAATTTGAAATCATTGAGTGATGAAGATGCATTTGCTCTTTTTAATCATTCAGCATACTTGGGAGATGTTCGCTCTCATGTTCCAGAAGACCTTGCAAGACAGGTAATAAAACATTGTAAAGGATTTCCTCTTGCAATTACAGTGGTTGGAAGATCACTTTGTGGGCAGCCTATAGAGATCTGGCAAAAAAGATTAATTGAATGGACTAAAGGTTCATCTATTCTTGATTCTGATCATGATTTGCTTGTTTGCCTCCAAAGCAGCCTTGATGCCTTGGAGAAACAAAGTATTACCATCAAGGAATGTTTCCTAGACCTTGGTTCATTTCCAGAAGACCAAAGAATACCTGCTACTACCCTCATTGATATATGGGCGGAGTCATATAAAATAGATGAAGACTGGCTTTGCATTGCAAACCTCTGTGAACTCACCAATAGAAATCTGGCTACTCTTATAATCACAAGGAAGGACCCCAGGGAGGTGGATGGCTATTACAGTGAACATTTTGTTACTCTGCATGACATTATTAGAGAATTGGCTATCTATCAGTCAAGTGTGGAATCAATAGAACAGAGAAAAAGGCTGATAATAGATATGTCTGGAGACAATTTTCCCGAGTGGTGGACGGAACAGAAATGTCAACCCATCAATGCTCACCTTTTATCTATCTCGACAGATGGAGACTTCTCATCTAAATGGCTCAACATGCAACTACCACAAGCAGAGGTTCTAATTCTGAACTTTCAGTCAAAGAAGTATGCCTTACCCGAATTTGTGGAGAAAATGGACAAATTAAAGGTTCTGATAGTCACAAAATACGGTCTTTTACCTGCTGAATTGGATAACTTTCCATTACTTGGTTCATTATCAAATCTAAAGAGAATTAGATTAGAACGAATAGCAATACCTTCCATGAGCAAGACCCCTGTACAGTTGAAGAGTTTAGAAAAGATATCATTATTCATGTGCAAAGTGGGTCAAGCCTTTGACAACTGTTCCATCCAACTTTTTAATCTATTGCCAAATGTAGTGGAAATGAACATTGATTACTGCAATGATTTGGTGAAATTGCCTGCCATGCTTTGTGACCTGATTTGCCTGAAAAAGCTTAGTATCACCAATTCTCACAAGCTATCTGTCTTACCTGAAGAAATAGGAAAGTTAGTGAACTTAAAAGTGTTGAGGCTTAGGTCTTGTACTGAATTGTCAGAGCTGCCAGACTCGATTCGGAACCTCAAATTGTTAAATTTTCTGGACATATCTGATTGCTTTAGTATGAAGGAGCTTCCTGAACATATTGGTGAAATGTGCAGCTTAGAAACGCTAAATATGAGACAATGCTCAAGACTGCAAGAGTTGCCTGCATCAGTTTTGGATCTTGAACAGTTGAAGGATGTGATTTGTGATGAAGAGACTGAAATATTATGGAACCCCTTCTTACCCATTCTCCAAAACTTACATATCAGGGTAGTGAAAGAAGATATTAACCTGAATTGGCTCCACACGTTTCCATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
Ion_trans

Protein Analysis

818

Amino Acids

93.34

Weight (kDa)

5.26

Isoelectric Point (pI)

44.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 3 - 137 2.1e-24 Arabidopsis broad-spectrum mildew resistance protein RPW8
NB-ARC PF00931 172 - 331 3.6e-16 NB-ARC domain
LRR_14 PF23598 673 - 735 4.9e-08 Leucine-rich repeat region
LRR_14 PF23598 722 - 800 1.1e-08 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000119)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g10570 FvH4_4g36390 FvH4_7g31840 FvH4_7g31840 FvH4_7g31850 FvH4_7g31850 FvH4_7g31861 FvH4_7g31861 FvH4_7g31950
malus_domestica MD01G1054600.v1.1 MD01G1221100.v1.1 MD01G1221400.v1.1 MD01G1221600.v1.1 MD01G1221800.v1.1 MD01G1222000.v1.1 MD01G1222100.v1.1 MD07G1140800.v1.1 MD07G1291200.v1.1 MD07G1291400.v1.1 MD07G1291500.v1.1 MD07G1291700.v1.1 MD07G1292500.v1.1 MD07G1292700.v1.1 MD07G1292800.v1.1 MD07G1292900.v1.1 MD08G1241600.v1.1 MD11G1083800.v1.1 MD15G1431600.v1.1
prunus_persica Prupe.1G017800_v2.0.a1 Prupe.1G017800_v2.0.a1 Prupe.1G162800_v2.0.a1 Prupe.2G312500_v2.0.a1 Prupe.2G312500_v2.0.a1 Prupe.2G312700_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092800_v2.0.a1 Prupe.4G092800_v2.0.a1 Prupe.6G142800_v2.0.a1 Prupe.6G142800_v2.0.a1 Prupe.6G143000_v2.0.a1 Prupe.6G143100_v2.0.a1 Prupe.6G143100_v2.0.a1 Prupe.6G143100_v2.0.a1 Prupe.6G143100_v2.0.a1
pyrus_communis pycom01g23050 pycom01g23070 pycom01g23090 pycom01g23100 pycom01g23110 pycom01g23120 pycom01g23170 pycom07g26410 pycom07g26440 pycom07g26470 pycom07g26480 pycom07g26490 pycom07g26500 pycom07g26510 pycom07g26530 pycom07g26540 pycom07g26570 pycom07g26580 pycom07g26590 pycom08g20940
rosa_chinensis RchiOBHm_Chr1g0324571 RchiOBHm_Chr1g0336181 RchiOBHm_Chr1g0381061 RchiOBHm_Chr1g0381091 RchiOBHm_Chr1g0381121 RchiOBHm_Chr1g0381131 RchiOBHm_Chr1g0381141 RchiOBHm_Chr1g0381161 RchiOBHm_Chr1g0381211 RchiOBHm_Chr2g0110361 RchiOBHm_Chr2g0120231 RchiOBHm_Chr3g0486281 RchiOBHm_Chr3g0486311 RchiOBHm_Chr3g0486401 RchiOBHm_Chr4g0409511 RchiOBHm_Chr4g0409521 RchiOBHm_Chr5g0017271 RchiOBHm_Chr5g0048781 RchiOBHm_Chr5g0048811
rosa_laevigata RLG00000002401 RLG00000008559 RLG00000017798 RLG00000017799 RLG00000023082 RLG00000023088 RLG00000026230 RLG00000026238 RLG00000026240 RLG00000026241 RLG00000026242 RLG00000026243 RLG00000026244 RLG00000026246 RLG00000026247 RLG00000026248 RLG00000029876 RLG00000030189 RLG00000034579
rosa_multiflora Rmu_co8101374.1_g000001 Rmu_co8309149.1_g000001 Rmu_co8450063.1_g000001 Rmu_sc0000441.1_g000094 Rmu_sc0000479.1_g000005 Rmu_sc0000647.1_g000006 Rmu_sc0001393.1_g000031 Rmu_sc0001423.1_g000037 Rmu_sc0001616.1_g000015 Rmu_sc0001857.1_g000011 Rmu_sc0002183.1_g000013 Rmu_sc0002183.1_g000028 Rmu_sc0002183.1_g000030 Rmu_sc0002688.1_g000003 Rmu_sc0002688.1_g000020 Rmu_sc0003801.1_g000003 Rmu_sc0003801.1_g000004 Rmu_sc0004125.1_g000006 Rmu_sc0004494.1_g000005 Rmu_sc0004494.1_g000006 Rmu_sc0004494.1_g000008 Rmu_sc0004494.1_g000009 Rmu_sc0005842.1_g000004 Rmu_sc0006872.1_g000001 Rmu_sc0006872.1_g000005 Rmu_sc0006872.1_g000009 Rmu_sc0006872.1_g000010 Rmu_sc0008053.1_g000026
rosa_roxburghii Rroxscaffold_159G00432960 Rroxscaffold_1G00033060 Rroxscaffold_2G00123150 Rroxscaffold_2G00123170 Rroxscaffold_2G00133500 Rroxscaffold_2G00133550 Rroxscaffold_4G00278650 Rroxscaffold_4G00278750 Rroxscaffold_4G00278790 Rroxscaffold_4G00278800 Rroxscaffold_4G00278810 Rroxscaffold_4G00325390 Rroxscaffold_5G00354010 Rroxscaffold_6G00395890 Rroxscaffold_6G00395900 Rroxscaffold_6G00395920
rosa_rugosa Rorug01G0046900.1 Rorug01G0427000 Rorug01G0427100 Rorug01G0427200 Rorug01G0427300 Rorug01G0427400 Rorug01G0427500 Rorug01G0427500 Rorug01G0427600 Rorug01G0427600 Rorug01G0427700 Rorug01G0427700 Rorug01G0427800 Rorug01G0428700 Rorug02G0166400 Rorug02G0166500 Rorug02G0166500 Rorug02G0166600 Rorug02G0166600 Rorug02G0166700 Rorug02G0167300 Rorug02G0167300 Rorug02G0167300 Rorug03G0224600 Rorug03G0224600 Rorug04G0094400 Rorug05G0241100 Rorug05G0241200
rosa_samantha Rh1BG407700 Rh1BG407900 Rh1BG409000 Rh1CG064700 Rh1CG420900 Rh1CG421000 Rh1CG421200 Rh1CG421300 Rh1CG421800 Rh1CG421900 Rh1CG422000 Rh1CG422200 Rh1CG422300 Rh1CG423600 Rh1DG437600 Rh1DG438900 Rh1DG439000 Rh1DG440300 Rh2AG219100 Rh2CG220700 Rh2DG225300 Rh3AG274400 Rh3AG274600 Rh3CG308900 Rh3DG305800 Rh4AG156400 Rh5AG129000 Rh5AG320900 Rh5CG139600
rosa_wichuraiana Rw1G005300 Rw1G039280 Rw1G039300 Rw1G039310 Rw1G039320 Rw1G039330 Rw1G039340 Rw1G039360 Rw1G039390 Rw2G016910 Rw2G022800 Rw3G024330 Rw3G024340 Rw3G024350 Rw3G024400 Rw4G012810 Rw5G030120 Rw5G030280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1394
AatII GACGTC 1 cut(s) 633
Acc36I ACCTGC 2 cut(s) 1294, 1777
Acc65I GGTACC 1 cut(s) 733
AccB1I GGYRCC 1 cut(s) 733
AccB7I CCANNNNNTGG 2 cut(s) 926, 1796
AciI CCGC 1 cut(s) 1313
AclWI GGATC 2 cut(s) 797, 2322
AcsI RAATTY 5 cut(s) 641, 779, 845, 1709, 2185
AcuI CTGAAG 1 cut(s) 2106
AcyI GRCGYC 1 cut(s) 630
AdeI CACNNNGTG 1 cut(s) 1100
AfaI GTAC 5 cut(s) 210, 268, 735, 1869, 2137
AfiI CCNNNNNNNGG 4 cut(s) 926, 1417, 1560, 1796
AflIII ACRYGT 1 cut(s) 2442
AgsI TTSAA 9 cut(s) 461, 520, 529, 646, 946, 1139, 1876, 2321, 2329
AjnI CCWGG 3 cut(s) 577, 729, 1412
AjuI GAANNNNNNNTTGG 6 cut(s) 359, 391, 584, 616, 913, 945
AloI GAACNNNNNNTCC 2 cut(s) 1135, 1167
AluBI AGCT 9 cut(s) 23, 232, 538, 709, 2047, 2071, 2152, 2224, 2253
AluI AGCT 9 cut(s) 23, 232, 538, 709, 2047, 2071, 2152, 2224, 2253
Alw21I GWGCWC 1 cut(s) 248
Alw26I GTCTC 7 cut(s) 143, 392, 621, 1536, 1614, 2266, 2345
AlwI GGATC 2 cut(s) 797, 2322
Ama87I CYCGRG 1 cut(s) 1553
AoxI GGCC 1 cut(s) 512
ApeKI GCWGC 5 cut(s) 772, 1105, 1200, 2152, 2250
ApoI RAATTY 5 cut(s) 641, 779, 845, 1709, 2185
AseI ATTAAT 1 cut(s) 1133
Asp700I GAANNNNTTC 3 cut(s) 539, 849, 2372
Asp718I GGTACC 1 cut(s) 733
AspS9I GGNCC 4 cut(s) 257, 406, 512, 1408
AsuHPI GGTGA 7 cut(s) 314, 910, 1360, 1586, 2014, 2047, 2252
AvaI CYCGRG 1 cut(s) 1553
AvaII GGWCC 3 cut(s) 257, 406, 1408
BanI GGYRCC 1 cut(s) 733
BbsI GAAGAC 3 cut(s) 1032, 1278, 1341
Bbv12I GWGCWC 1 cut(s) 248
BbvI GCAGC 5 cut(s) 759, 1117, 1212, 2139, 2262
BccI CCATC 7 cut(s) 503, 617, 1241, 1418, 1592, 1610, 1946
BciT130I CCWGG 3 cut(s) 579, 731, 1414
BciVI GTATCC 1 cut(s) 426
BclI TGATCA 2 cut(s) 141, 1171
BcoDI GTCTC 7 cut(s) 143, 392, 621, 1536, 1614, 2266, 2345
BfaI CTAG 3 cut(s) 122, 299, 1250
BfmI CTRYAG 2 cut(s) 129, 1110
BfuAI ACCTGC 2 cut(s) 1294, 1777
BfuI GTATCC 1 cut(s) 426
BglII AGATCT 3 cut(s) 113, 891, 1116
BisI GCNGC 5 cut(s) 773, 1106, 1201, 2153, 2251
BlsI GCNGC 5 cut(s) 774, 1107, 1202, 2154, 2252
BmcAI AGTACT 1 cut(s) 268
Bme1390I CCNGG 3 cut(s) 579, 731, 1414
Bme18I GGWCC 3 cut(s) 257, 406, 1408
BmeT110I CYCGRG 1 cut(s) 1553
BmgT120I GGNCC 4 cut(s) 257, 406, 512, 1408
BmiI GGNNCC 6 cut(s) 727, 735, 1410, 2171, 2369, 2438
BmrFI CCNGG 3 cut(s) 579, 731, 1414
BmsI GCATC 5 cut(s) 41, 43, 955, 1198, 2312
BoxI GACNNNNGTC 1 cut(s) 404
BpiI GAAGAC 3 cut(s) 1032, 1278, 1341
BpmI CTGGAG 2 cut(s) 600, 1560
Bpu10I CCTNAGC 1 cut(s) 2126
BpuEI CTTGAG 2 cut(s) 875, 2266
BsaBI GATNNNNATC 1 cut(s) 1170
BsaHI GRCGYC 1 cut(s) 630
BsaJI CCNNGG 7 cut(s) 100, 375, 729, 1212, 1255, 1412, 1413
BsaXI ACNNNNNCTCC 2 cut(s) 2370, 2400
Bsc4I CCNNNNNNNGG 4 cut(s) 926, 1417, 1560, 1796
Bse1I ACTGG 1 cut(s) 1343
Bse3DI GCAATG 2 cut(s) 1347, 1999
Bse8I GATNNNNATC 1 cut(s) 1170
BseBI CCWGG 3 cut(s) 579, 731, 1414
BseDI CCNNGG 7 cut(s) 100, 375, 729, 1212, 1255, 1412, 1413
BseGI GGATG 8 cut(s) 166, 193, 514, 590, 829, 1429, 1938, 2338
BseJI GATNNNNATC 1 cut(s) 1170
BseLI CCNNNNNNNGG 4 cut(s) 926, 1417, 1560, 1796
BseMI GCAATG 2 cut(s) 1347, 1999
BseNI ACTGG 1 cut(s) 1343
BseRI GAGGAG 1 cut(s) 30
BseXI GCAGC 5 cut(s) 759, 1117, 1212, 2139, 2262
BsgI GTGCAG 2 cut(s) 758, 2269
BshFI GGCC 1 cut(s) 514
BshNI GGYRCC 1 cut(s) 733
BsiHKAI GWGCWC 1 cut(s) 248
BsiHKCI CYCGRG 1 cut(s) 1553
BslFI GGGAC 1 cut(s) 810
BslI CCNNNNNNNGG 4 cut(s) 926, 1417, 1560, 1796
BsmAI GTCTC 7 cut(s) 143, 392, 621, 1536, 1614, 2266, 2345
BsmBI CGTCTC 1 cut(s) 621
BsmFI GGGAC 1 cut(s) 810
BsnI GGCC 1 cut(s) 514
BsoBI CYCGRG 1 cut(s) 1553
Bsp1286I GDGCHC 1 cut(s) 248
Bsp1407I TGTACA 1 cut(s) 1867
Bsp143I GATC 8 cut(s) 113, 141, 802, 891, 1091, 1116, 1171, 2314
BspACI CCGC 1 cut(s) 1313
BspANI GGCC 1 cut(s) 514
BspHI TCATGA 1 cut(s) 1174
BspLI GGNNCC 6 cut(s) 727, 735, 1410, 2171, 2369, 2438
BspMI ACCTGC 2 cut(s) 1294, 1777
BspPI GGATC 2 cut(s) 797, 2322
BspQI GCTCTTC 1 cut(s) 699
BspT107I GGYRCC 1 cut(s) 733
BsrDI GCAATG 2 cut(s) 1347, 1999
BsrGI TGTACA 1 cut(s) 1867
BsrI ACTGG 1 cut(s) 1343
BssECI CCNNGG 7 cut(s) 100, 375, 729, 1212, 1255, 1412, 1413
BssMI GATC 8 cut(s) 113, 141, 802, 891, 1091, 1116, 1171, 2314
BssNI GRCGYC 1 cut(s) 630
BssT1I CCWWGG 4 cut(s) 100, 375, 1212, 1255
Bst2UI CCWGG 3 cut(s) 579, 731, 1414
Bst6I CTCTTC 4 cut(s) 213, 699, 1871, 2343
BstACI GRCGYC 1 cut(s) 630
BstAPI GCANNNNNTGC 1 cut(s) 2296
BstAUI TGTACA 1 cut(s) 1867
BstC8I GCNNGC 2 cut(s) 2013, 2301
BstDEI CTNAG 5 cut(s) 249, 402, 2048, 2126, 2254
BstEII GGTNACC 1 cut(s) 379
BstF5I GGATG 8 cut(s) 166, 193, 514, 590, 829, 1429, 1938, 2338
BstKTI GATC 8 cut(s) 116, 144, 805, 894, 1094, 1119, 1174, 2317
BstMAI GTCTC 7 cut(s) 143, 392, 621, 1536, 1614, 2266, 2345
BstMBI GATC 8 cut(s) 113, 141, 802, 891, 1091, 1116, 1171, 2314
BstMWI GCNNNNNNNGC 5 cut(s) 29, 1197, 1645, 2017, 2296
BstNI CCWGG 3 cut(s) 579, 731, 1414
BstNSI RCATGY 1 cut(s) 1648
BstPAI GACNNNNGTC 1 cut(s) 404
BstPI GGTNACC 1 cut(s) 379
BstSCI CCNGG 3 cut(s) 577, 729, 1412
BstSFI CTRYAG 2 cut(s) 129, 1110
BstV1I GCAGC 5 cut(s) 759, 1117, 1212, 2139, 2262
BstV2I GAAGAC 3 cut(s) 1032, 1278, 1341
BstX2I RGATCY 5 cut(s) 113, 802, 891, 1116, 2314
BstXI CCANNNNNNTGG 1 cut(s) 1420
BstYI RGATCY 5 cut(s) 113, 802, 891, 1116, 2314
BsuI GTATCC 1 cut(s) 426
BsuRI GGCC 1 cut(s) 514
BtsCI GGATG 8 cut(s) 166, 193, 514, 590, 829, 1429, 1938, 2338
BtsIMutI CAGTG 5 cut(s) 44, 137, 550, 1086, 1441
BveI ACCTGC 2 cut(s) 1294, 1777
Cac8I GCNNGC 2 cut(s) 2013, 2301
CciI TCATGA 1 cut(s) 1174
Cfr13I GGNCC 4 cut(s) 257, 406, 512, 1408
Csp6I GTAC 5 cut(s) 209, 267, 734, 1868, 2136
CspCI CAANNNNNGTGG 4 cut(s) 1062, 1097, 1950, 1985
CviAII CATG 8 cut(s) 749, 1016, 1175, 1457, 1645, 1852, 1903, 2017
CviQI GTAC 5 cut(s) 209, 267, 734, 1868, 2136
DdeI CTNAG 5 cut(s) 249, 402, 2048, 2126, 2254
DpnI GATC 8 cut(s) 115, 143, 804, 893, 1093, 1118, 1173, 2316
DpnII GATC 8 cut(s) 113, 141, 802, 891, 1091, 1116, 1171, 2314
DraI TTTAAA 1 cut(s) 91
DraIII CACNNNGTG 1 cut(s) 1100
Eam1104I CTCTTC 4 cut(s) 213, 699, 1871, 2343
EarI CTCTTC 4 cut(s) 213, 699, 1871, 2343
EciI GGCGGA 1 cut(s) 1328
Eco130I CCWWGG 4 cut(s) 100, 375, 1212, 1255
Eco32I GATATC 3 cut(s) 109, 555, 1893
Eco47I GGWCC 3 cut(s) 257, 406, 1408
Eco57I CTGAAG 1 cut(s) 2106
Eco88I CYCGRG 1 cut(s) 1553
Eco91I GGTNACC 1 cut(s) 379
EcoO109I RGGNCCY 1 cut(s) 1408
EcoO65I GGTNACC 1 cut(s) 379
EcoRII CCWGG 3 cut(s) 577, 729, 1412
EcoRV GATATC 3 cut(s) 109, 555, 1893
EcoT14I CCWWGG 4 cut(s) 100, 375, 1212, 1255
EcoT22I ATGCAT 1 cut(s) 970
ErhI CCWWGG 4 cut(s) 100, 375, 1212, 1255
Esp3I CGTCTC 1 cut(s) 621
FaeI CATG 8 cut(s) 752, 1019, 1178, 1460, 1648, 1855, 1906, 2020
FaqI GGGAC 1 cut(s) 810
FatI CATG 8 cut(s) 748, 1015, 1174, 1456, 1644, 1851, 1902, 2016
FbaI TGATCA 2 cut(s) 141, 1171
Fnu4HI GCNGC 5 cut(s) 773, 1106, 1201, 2153, 2251
FokI GGATG 8 cut(s) 173, 200, 521, 597, 836, 1436, 1925, 2345
Fsp4HI GCNGC 5 cut(s) 773, 1106, 1201, 2153, 2251
FspBI CTAG 3 cut(s) 122, 299, 1250
GluI GCNGC 5 cut(s) 773, 1106, 1201, 2153, 2251
GsuI CTGGAG 2 cut(s) 600, 1560
HaeIII GGCC 1 cut(s) 514
Hin1I GRCGYC 1 cut(s) 630
Hin1II CATG 8 cut(s) 752, 1019, 1178, 1460, 1648, 1855, 1906, 2020
HincII GTYRAC 1 cut(s) 1580
HindII GTYRAC 1 cut(s) 1580
HindIII AAGCTT 3 cut(s) 21, 536, 2045
HinfI GANTC 7 cut(s) 125, 839, 1166, 1316, 1499, 2159, 2164
HphI GGTGA 7 cut(s) 314, 910, 1360, 1586, 2014, 2047, 2252
Hpy166II GTNNAC 5 cut(s) 1364, 1439, 1563, 1580, 2107
Hpy8I GTNNAC 5 cut(s) 1364, 1439, 1563, 1580, 2107
HpyAV CCTTC 7 cut(s) 455, 514, 1399, 1857, 2212, 2323, 2383
HpyCH4IV ACGT 2 cut(s) 630, 2444
HpyF10VI GCNNNNNNNGC 5 cut(s) 29, 1197, 1645, 2017, 2296
HpyF3I CTNAG 5 cut(s) 249, 402, 2048, 2126, 2254
HpySE526I ACGT 2 cut(s) 630, 2444
Hsp92I GRCGYC 1 cut(s) 630
Hsp92II CATG 8 cut(s) 752, 1019, 1178, 1460, 1648, 1855, 1906, 2020
KpnI GGTACC 1 cut(s) 737
Ksp22I TGATCA 2 cut(s) 141, 1171
Kzo9I GATC 8 cut(s) 113, 141, 802, 891, 1091, 1116, 1171, 2314
LguI GCTCTTC 1 cut(s) 699
LmnI GCTCC 3 cut(s) 580, 2221, 2442
Lsp1109I GCAGC 5 cut(s) 759, 1117, 1212, 2139, 2262
LweI GCATC 5 cut(s) 41, 43, 955, 1198, 2312
MaeI CTAG 3 cut(s) 122, 299, 1250
MaeII ACGT 2 cut(s) 630, 2444
MaeIII GTNAC 6 cut(s) 379, 664, 898, 1447, 1747, 2024
MalI GATC 8 cut(s) 115, 143, 804, 893, 1093, 1118, 1173, 2316
MboI GATC 8 cut(s) 113, 141, 802, 891, 1091, 1116, 1171, 2314
MflI RGATCY 5 cut(s) 113, 802, 891, 1116, 2314
MhlI GDGCHC 1 cut(s) 248
MlyI GAGTC 2 cut(s) 1325, 2153
MmeI TCCRAC 6 cut(s) 153, 312, 481, 769, 1066, 1966
Mph1103I ATGCAT 1 cut(s) 970
MroXI GAANNNNTTC 3 cut(s) 539, 849, 2372
MslI CAYNNNNRTG 2 cut(s) 1633, 2238
MspR9I CCNGG 3 cut(s) 579, 731, 1414
MvaI CCWGG 3 cut(s) 579, 731, 1414
MwoI GCNNNNNNNGC 5 cut(s) 29, 1197, 1645, 2017, 2296
NdeII GATC 8 cut(s) 113, 141, 802, 891, 1091, 1116, 1171, 2314
NlaIII CATG 8 cut(s) 752, 1019, 1178, 1460, 1648, 1855, 1906, 2020
NlaIV GGNNCC 6 cut(s) 727, 735, 1410, 2171, 2369, 2438
NmuCI GTSAC 3 cut(s) 898, 1747, 2024
NsiI ATGCAT 1 cut(s) 970
NspI RCATGY 1 cut(s) 1648
PagI TCATGA 1 cut(s) 1174
PasI CCCWGGG 1 cut(s) 1413
PciSI GCTCTTC 1 cut(s) 699
PdmI GAANNNNTTC 3 cut(s) 539, 849, 2372
PfeI GAWTC 5 cut(s) 125, 839, 1166, 1499, 2164
PflMI CCANNNNNTGG 2 cut(s) 926, 1796
PfoI TCCNGGA 1 cut(s) 577
PkrI GCNGC 5 cut(s) 774, 1107, 1202, 2154, 2252
PleI GAGTC 2 cut(s) 1324, 2153
PpsI GAGTC 2 cut(s) 1324, 2153
PpuMI RGGWCCY 1 cut(s) 1408
PshAI GACNNNNGTC 1 cut(s) 404
PshBI ATTAAT 1 cut(s) 1133
PsiI TTATAA 1 cut(s) 1394
Psp5II RGGWCCY 1 cut(s) 1408
Psp6I CCWGG 3 cut(s) 577, 729, 1412
PspEI GGTNACC 1 cut(s) 379
PspGI CCWGG 3 cut(s) 577, 729, 1412
PspN4I GGNNCC 6 cut(s) 727, 735, 1410, 2171, 2369, 2438
PspPI GGNCC 4 cut(s) 257, 406, 512, 1408
PspPPI RGGWCCY 1 cut(s) 1408
PsrI GAACNNNNNNTAC 4 cut(s) 448, 480, 1970, 2002
PsuI RGATCY 5 cut(s) 113, 802, 891, 1116, 2314
RsaI GTAC 5 cut(s) 210, 268, 735, 1869, 2137
RsaNI GTAC 5 cut(s) 209, 267, 734, 1868, 2136
RseI CAYNNNNRTG 2 cut(s) 1633, 2238
SapI GCTCTTC 1 cut(s) 699
SatI GCNGC 5 cut(s) 773, 1106, 1201, 2153, 2251
Sau3AI GATC 8 cut(s) 113, 141, 802, 891, 1091, 1116, 1171, 2314
Sau96I GGNCC 4 cut(s) 257, 406, 512, 1408
ScaI AGTACT 1 cut(s) 268
SchI GAGTC 2 cut(s) 1325, 2153
ScrFI CCNGG 3 cut(s) 579, 731, 1414
SduI GDGCHC 1 cut(s) 248
SfaNI GCATC 5 cut(s) 41, 43, 955, 1198, 2312
SfcI CTRYAG 2 cut(s) 129, 1110
SinI GGWCC 3 cut(s) 257, 406, 1408
SmiMI CAYNNNNRTG 2 cut(s) 1633, 2238
SmlI CTYRAG 2 cut(s) 854, 2281
SmoI CTYRAG 2 cut(s) 854, 2281
SsiI CCGC 1 cut(s) 1313
SspI AATATT 3 cut(s) 394, 427, 2361
SspMI CTAG 3 cut(s) 122, 299, 1250
StyD4I CCNGG 3 cut(s) 577, 729, 1412
StyI CCWWGG 4 cut(s) 100, 375, 1212, 1255
TaiI ACGT 2 cut(s) 633, 2447
TaqI TCGA 2 cut(s) 1610, 2162
TatI WGTACW 4 cut(s) 208, 266, 1867, 2135
TfiI GAWTC 5 cut(s) 125, 839, 1166, 1499, 2164
TscAI CASTG 5 cut(s) 51, 144, 550, 1086, 1441
TseFI GTSAC 3 cut(s) 898, 1747, 2024
TseI GCWGC 5 cut(s) 772, 1105, 1200, 2152, 2250
Tsp45I GTSAC 3 cut(s) 898, 1747, 2024
TspGWI ACGGA 1 cut(s) 1580
TspRI CASTG 5 cut(s) 51, 144, 550, 1086, 1441
Van91I CCANNNNNTGG 2 cut(s) 926, 1796
VpaK11BI GGWCC 3 cut(s) 257, 406, 1408
VspI ATTAAT 1 cut(s) 1133
XapI RAATTY 5 cut(s) 641, 779, 845, 1709, 2185
XceI RCATGY 1 cut(s) 1648
XcmI CCANNNNNNNNNTGG 1 cut(s) 1378
XmnI GAANNNNTTC 3 cut(s) 539, 849, 2372
XspI CTAG 3 cut(s) 122, 299, 1250
ZraI GACGTC 1 cut(s) 631
ZrmI AGTACT 1 cut(s) 268
Zsp2I ATGCAT 1 cut(s) 970
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.