Rw5G030280

Cyclic nucleotide-gated ion channel 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000119
Physical Location & Seq
Reverse (-)
47945445 .. 47948573
3129 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G030280.1

Sequence Viewer

Length: 1878 bp
ATGATTCAAAGCTCCGTATATGATGTCATTGCGAAAGCTGCACTATCACACCCGAGAGAAATGGAATGGACAGCGACTAAAGGACTTCTTGCAATTTTGAAATCACACCGGACTCTCATACTTACAATTTCATGTGTGATTGCAGTCATAGTGGATCCATTGTTCTTTTACCTTCCAATAATTGATGGGAAAAGAAAGTGCATTGGAATGGACACAATATTGAGGAATGTGGCACTTATTTTCCGATCACTCACCGATATTGGATTCATAGTGAATATTATACATAAAATTCGTGAAGCTGTGAATAAAACAAGAGCAGATTGTCTTAAAAACATTGAAAAAATGACTGCTGAAGAACGAAATCGAGGTGAGAGCGGAAATAAGTTTGCCAAGTCATTAGCTCGTAACTTGTCGTGGCACAACATCTTAATCGACTTTTTGGCTGTTCTTCCCATCCCCCAAGTGATAATAGTAGCTGTTAGAAGCTCTAGATGTTTGGATAAAAGAAAGATTCTAAACTTTCTTCTTCTCGCCCAGTATCTGCCGAGGGTTTATCGAATGCGTCATTCATCTATACAACTTAGACCAAGAACTGGAATATGGGTTAAAGGTTCATTCTATTTTTTCTTGTACATCCTTGCCAGCCATGTACTTGGAGCCTTTTGGTATTATTTTTCTATTCAACAAGAGACATCTTGTTGGCATCGGGCTTGCAAAAAGCATGGTGGAGTAAAATGTAATTTTTACTGCCATGAAAACATGACTTCAAGAGATTTGACATTCCCCATCAATTCTATAGATAAATATTGCCTTGTAGATGTTCCGGCAAATGAAACTGCACCATTTGATTTTGGAATCTTTCTTGATTCTCTTAAGAATAAGAACACGGCATCAATACAATTTCCAAAGAAGTTTTTCTACTCTTTTTGGTGGGGACTTCGAAATCTGAGTAATTTTGGCACAAATTTGCAAACAAGTACTTATGTGTGGGAAAACTGCTTTGCAATTCTGATTTCAATCATTGGCCTGCTATTATTCTTATATCTCATTGGAAAAGTACAGACATTTATTTCGATGCAAACTGCGAAGTCCGAGGAGAGACGAAAAAAATTCAAATCAGAAGAGAGAAGGAAAAAATTCGAAGCAGAAGAGAGCAGAAAAAAAATATCAAAAGAGTTAGATATACAAATATGGATGACAAGATATGGTTTCAAGAGAGATATGATGAAACAAATCAGAGTAGAGATAAATGAAAAGTTGGAAGAAATACGAGATGCAGATATAGAGAATCTATTCGCTGTTCTTCCCATGAAATATCGAAAACATCTTAAACATTCTCTTTGCATGGAAATGCTGAGCAAGGTGCCAAAGCTTAGAACTCTGAAAGATGAAGCTTTGAAACCGATCTGCGATTGTCTAAAGCCAGTGGTCTATCAGGATAATAGCTTTGTTTTCCAAGAAGGAGAACCACTTGATCGCATTCTCTTCATCACAGAAGGCTTAATATGGACATACCAGGCGGCGGGCGCTACTGATAGTAGTAGCACTAGCCAAAATGGAAGAGGAAAAACAGGTTTCAGTTTTCTTCAGAAAGGTGGATTTTACGGAGACGATCAACTTCTGAGTTGGGTTATTGCATCACAACAGAATCTTCCCTTATTTAGCAACCTTCCAATCTCCAAAGTGAATGTGAAATGCCATGCAAAAGTAGAAGGCTTTGTACTCATGGCCAAGGACTTGAAAATTGTGGTCTCCACTAATTACAGAGTTTATTGGGGTCTCCACAATTCTTCTCAGGAAAGAGAGAAGGCATTAATTCAAGCCATAAAGAACGTACGGGATCGTCGTAAACAGATAGACCAAAGGGAAATCCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
Ion_trans

Protein Analysis

625

Amino Acids

72.48

Weight (kDa)

9.6

Isoelectric Point (pI)

42.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ion_trans PF00520 41 - 364 3.1e-08 Ion transport protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000119)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g10570 FvH4_4g36390 FvH4_7g31840 FvH4_7g31840 FvH4_7g31850 FvH4_7g31850 FvH4_7g31861 FvH4_7g31861 FvH4_7g31950
malus_domestica MD01G1054600.v1.1 MD01G1221100.v1.1 MD01G1221400.v1.1 MD01G1221600.v1.1 MD01G1221800.v1.1 MD01G1222000.v1.1 MD01G1222100.v1.1 MD07G1140800.v1.1 MD07G1291200.v1.1 MD07G1291400.v1.1 MD07G1291500.v1.1 MD07G1291700.v1.1 MD07G1292500.v1.1 MD07G1292700.v1.1 MD07G1292800.v1.1 MD07G1292900.v1.1 MD08G1241600.v1.1 MD11G1083800.v1.1 MD15G1431600.v1.1
prunus_persica Prupe.1G017800_v2.0.a1 Prupe.1G017800_v2.0.a1 Prupe.1G162800_v2.0.a1 Prupe.2G312500_v2.0.a1 Prupe.2G312500_v2.0.a1 Prupe.2G312700_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092800_v2.0.a1 Prupe.4G092800_v2.0.a1 Prupe.6G142800_v2.0.a1 Prupe.6G142800_v2.0.a1 Prupe.6G143000_v2.0.a1 Prupe.6G143100_v2.0.a1 Prupe.6G143100_v2.0.a1 Prupe.6G143100_v2.0.a1 Prupe.6G143100_v2.0.a1
pyrus_communis pycom01g23050 pycom01g23070 pycom01g23090 pycom01g23100 pycom01g23110 pycom01g23120 pycom01g23170 pycom07g26410 pycom07g26440 pycom07g26470 pycom07g26480 pycom07g26490 pycom07g26500 pycom07g26510 pycom07g26530 pycom07g26540 pycom07g26570 pycom07g26580 pycom07g26590 pycom08g20940
rosa_chinensis RchiOBHm_Chr1g0324571 RchiOBHm_Chr1g0336181 RchiOBHm_Chr1g0381061 RchiOBHm_Chr1g0381091 RchiOBHm_Chr1g0381121 RchiOBHm_Chr1g0381131 RchiOBHm_Chr1g0381141 RchiOBHm_Chr1g0381161 RchiOBHm_Chr1g0381211 RchiOBHm_Chr2g0110361 RchiOBHm_Chr2g0120231 RchiOBHm_Chr3g0486281 RchiOBHm_Chr3g0486311 RchiOBHm_Chr3g0486401 RchiOBHm_Chr4g0409511 RchiOBHm_Chr4g0409521 RchiOBHm_Chr5g0017271 RchiOBHm_Chr5g0048781 RchiOBHm_Chr5g0048811
rosa_laevigata RLG00000002401 RLG00000008559 RLG00000017798 RLG00000017799 RLG00000023082 RLG00000023088 RLG00000026230 RLG00000026238 RLG00000026240 RLG00000026241 RLG00000026242 RLG00000026243 RLG00000026244 RLG00000026246 RLG00000026247 RLG00000026248 RLG00000029876 RLG00000030189 RLG00000034579
rosa_multiflora Rmu_co8101374.1_g000001 Rmu_co8309149.1_g000001 Rmu_co8450063.1_g000001 Rmu_sc0000441.1_g000094 Rmu_sc0000479.1_g000005 Rmu_sc0000647.1_g000006 Rmu_sc0001393.1_g000031 Rmu_sc0001423.1_g000037 Rmu_sc0001616.1_g000015 Rmu_sc0001857.1_g000011 Rmu_sc0002183.1_g000013 Rmu_sc0002183.1_g000028 Rmu_sc0002183.1_g000030 Rmu_sc0002688.1_g000003 Rmu_sc0002688.1_g000020 Rmu_sc0003801.1_g000003 Rmu_sc0003801.1_g000004 Rmu_sc0004125.1_g000006 Rmu_sc0004494.1_g000005 Rmu_sc0004494.1_g000006 Rmu_sc0004494.1_g000008 Rmu_sc0004494.1_g000009 Rmu_sc0005842.1_g000004 Rmu_sc0006872.1_g000001 Rmu_sc0006872.1_g000005 Rmu_sc0006872.1_g000009 Rmu_sc0006872.1_g000010 Rmu_sc0008053.1_g000026
rosa_roxburghii Rroxscaffold_159G00432960 Rroxscaffold_1G00033060 Rroxscaffold_2G00123150 Rroxscaffold_2G00123170 Rroxscaffold_2G00133500 Rroxscaffold_2G00133550 Rroxscaffold_4G00278650 Rroxscaffold_4G00278750 Rroxscaffold_4G00278790 Rroxscaffold_4G00278800 Rroxscaffold_4G00278810 Rroxscaffold_4G00325390 Rroxscaffold_5G00354010 Rroxscaffold_6G00395890 Rroxscaffold_6G00395900 Rroxscaffold_6G00395920
rosa_rugosa Rorug01G0046900.1 Rorug01G0427000 Rorug01G0427100 Rorug01G0427200 Rorug01G0427300 Rorug01G0427400 Rorug01G0427500 Rorug01G0427500 Rorug01G0427600 Rorug01G0427600 Rorug01G0427700 Rorug01G0427700 Rorug01G0427800 Rorug01G0428700 Rorug02G0166400 Rorug02G0166500 Rorug02G0166500 Rorug02G0166600 Rorug02G0166600 Rorug02G0166700 Rorug02G0167300 Rorug02G0167300 Rorug02G0167300 Rorug03G0224600 Rorug03G0224600 Rorug04G0094400 Rorug05G0241100 Rorug05G0241200
rosa_samantha Rh1BG407700 Rh1BG407900 Rh1BG409000 Rh1CG064700 Rh1CG420900 Rh1CG421000 Rh1CG421200 Rh1CG421300 Rh1CG421800 Rh1CG421900 Rh1CG422000 Rh1CG422200 Rh1CG422300 Rh1CG423600 Rh1DG437600 Rh1DG438900 Rh1DG439000 Rh1DG440300 Rh2AG219100 Rh2CG220700 Rh2DG225300 Rh3AG274400 Rh3AG274600 Rh3CG308900 Rh3DG305800 Rh4AG156400 Rh5AG129000 Rh5AG320900 Rh5CG139600
rosa_wichuraiana Rw1G005300 Rw1G039280 Rw1G039300 Rw1G039310 Rw1G039320 Rw1G039330 Rw1G039340 Rw1G039360 Rw1G039390 Rw2G016910 Rw2G022800 Rw3G024330 Rw3G024340 Rw3G024350 Rw3G024400 Rw4G012810 Rw5G030120 Rw5G030280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1363
AccB7I CCANNNNNTGG 1 cut(s) 593
AccBSI CCGCTC 1 cut(s) 375
AciI CCGC 3 cut(s) 375, 1520, 1523
AclWI GGATC 3 cut(s) 149, 162, 1848
AcoI YGGCCR 1 cut(s) 1728
AcsI RAATTY 4 cut(s) 288, 964, 1109, 1136
AcuI CTGAAG 2 cut(s) 372, 1571
AfaI GTAC 6 cut(s) 632, 651, 979, 1059, 1722, 1836
AfiI CCNNNNNNNGG 2 cut(s) 593, 1522
AflII CTTAAG 1 cut(s) 872
AjnI CCWGG 1 cut(s) 1515
AloI GAACNNNNNNTCC 2 cut(s) 146, 178
AluBI AGCT 9 cut(s) 12, 38, 299, 401, 476, 486, 1372, 1394, 1446
AluI AGCT 9 cut(s) 12, 38, 299, 401, 476, 486, 1372, 1394, 1446
Alw26I GTCTC 5 cut(s) 683, 1093, 1602, 1756, 1784
AlwI GGATC 3 cut(s) 149, 162, 1848
AlwNI CAGNNNCTG 1 cut(s) 541
Ama87I CYCGRG 1 cut(s) 52
AoxI GGCC 2 cut(s) 1024, 1728
ApeKI GCWGC 1 cut(s) 38
ApoI RAATTY 4 cut(s) 288, 964, 1109, 1136
ArsI GACNNNNNNTTYG 2 cut(s) 1054, 1086
AseI ATTAAT 1 cut(s) 1814
Asp700I GAANNNNTTC 3 cut(s) 914, 1136, 1292
AspLEI GCGC 1 cut(s) 1529
AsuHPI GGTGA 2 cut(s) 244, 380
AsuII TTCGAA 2 cut(s) 940, 1140
AvaI CYCGRG 1 cut(s) 52
BalI TGGCCA 1 cut(s) 1730
BamHI GGATCC 1 cut(s) 154
BanI GGYRCC 1 cut(s) 1363
BarI GAAGNNNNNNTAC 4 cut(s) 902, 934, 1704, 1736
BbvI GCAGC 1 cut(s) 25
BccI CCATC 3 cut(s) 179, 461, 794
BceAI ACGGC 1 cut(s) 903
BciT130I CCWGG 1 cut(s) 1517
BcoDI GTCTC 5 cut(s) 683, 1093, 1602, 1756, 1784
BfaI CTAG 2 cut(s) 489, 1548
BfmI CTRYAG 1 cut(s) 795
BfoI RGCGCY 1 cut(s) 1530
BfrI CTTAAG 1 cut(s) 872
BisI GCNGC 2 cut(s) 39, 1521
BlpI GCTNAGC 1 cut(s) 1355
BlsI GCNGC 2 cut(s) 40, 1522
BmcAI AGTACT 1 cut(s) 979
Bme1390I CCNGG 1 cut(s) 1517
BmeT110I CYCGRG 1 cut(s) 52
BmiI GGNNCC 3 cut(s) 156, 658, 1365
BmrFI CCNGG 1 cut(s) 1517
BmrI ACTGGG 1 cut(s) 529
BmsI GCATC 5 cut(s) 712, 899, 1065, 1264, 1646
BmuI ACTGGG 1 cut(s) 529
Bpu1102I GCTNAGC 1 cut(s) 1355
Bpu14I TTCGAA 2 cut(s) 940, 1140
BsaBI GATNNNNATC 1 cut(s) 1016
BsaI GGTCTC 2 cut(s) 1756, 1784
BsaJI CCNNGG 3 cut(s) 545, 1092, 1731
BsaWI WCCGGW 1 cut(s) 108
Bsc4I CCNNNNNNNGG 2 cut(s) 593, 1522
Bse1I ACTGG 3 cut(s) 535, 598, 1424
Bse3DI GCAATG 1 cut(s) 27
Bse8I GATNNNNATC 1 cut(s) 1016
BseBI CCWGG 1 cut(s) 1517
BseDI CCNNGG 3 cut(s) 545, 1092, 1731
BseGI GGATG 3 cut(s) 453, 633, 1200
BseJI GATNNNNATC 1 cut(s) 1016
BseLI CCNNNNNNNGG 2 cut(s) 593, 1522
BseMI GCAATG 1 cut(s) 27
BseMII CTCAG 4 cut(s) 938, 1346, 1613, 1808
BseNI ACTGG 3 cut(s) 535, 598, 1424
BseRI GAGGAG 1 cut(s) 1109
BseXI GCAGC 1 cut(s) 25
BsgI GTGCAG 2 cut(s) 24, 822
BshFI GGCC 2 cut(s) 1026, 1730
BshNI GGYRCC 1 cut(s) 1363
BsiHKCI CYCGRG 1 cut(s) 52
BsiSI CCGG 2 cut(s) 109, 824
BsiWI CGTACG 1 cut(s) 1834
BslFI GGGAC 1 cut(s) 948
BslI CCNNNNNNNGG 2 cut(s) 593, 1522
BsmAI GTCTC 5 cut(s) 683, 1093, 1602, 1756, 1784
BsmBI CGTCTC 2 cut(s) 1093, 1602
BsmFI GGGAC 1 cut(s) 948
BsmI GAATGC 2 cut(s) 564, 1479
BsnI GGCC 2 cut(s) 1026, 1730
Bso31I GGTCTC 2 cut(s) 1756, 1784
BsoBI CYCGRG 1 cut(s) 52
Bsp119I TTCGAA 2 cut(s) 940, 1140
Bsp1407I TGTACA 1 cut(s) 630
Bsp143I GATC 6 cut(s) 154, 245, 1404, 1474, 1612, 1840
Bsp1720I GCTNAGC 1 cut(s) 1355
BspACI CCGC 3 cut(s) 375, 1520, 1523
BspANI GGCC 2 cut(s) 1026, 1730
BspCNI CTCAG 4 cut(s) 939, 1347, 1614, 1807
BspLI GGNNCC 3 cut(s) 156, 658, 1365
BspPI GGATC 3 cut(s) 149, 162, 1848
BspT104I TTCGAA 2 cut(s) 940, 1140
BspT107I GGYRCC 1 cut(s) 1363
BspTI CTTAAG 1 cut(s) 872
BspTNI GGTCTC 2 cut(s) 1756, 1784
BsrBI CCGCTC 1 cut(s) 375
BsrDI GCAATG 1 cut(s) 27
BsrGI TGTACA 1 cut(s) 630
BsrI ACTGG 3 cut(s) 535, 598, 1424
BssECI CCNNGG 3 cut(s) 545, 1092, 1731
BssMI GATC 6 cut(s) 154, 245, 1404, 1474, 1612, 1840
BssT1I CCWWGG 1 cut(s) 1731
Bst2UI CCWGG 1 cut(s) 1517
Bst6I CTCTTC 4 cut(s) 1116, 1143, 1490, 1555
BstAFI CTTAAG 1 cut(s) 872
BstAUI TGTACA 1 cut(s) 630
BstBI TTCGAA 2 cut(s) 940, 1140
BstC8I GCNNGC 4 cut(s) 643, 712, 1028, 1525
BstDEI CTNAG 6 cut(s) 581, 947, 1355, 1373, 1622, 1794
BstF5I GGATG 3 cut(s) 453, 633, 1200
BstH2I RGCGCY 1 cut(s) 1530
BstHHI GCGC 1 cut(s) 1529
BstKTI GATC 6 cut(s) 157, 248, 1407, 1477, 1615, 1843
BstMAI GTCTC 5 cut(s) 683, 1093, 1602, 1756, 1784
BstMBI GATC 6 cut(s) 154, 245, 1404, 1474, 1612, 1840
BstMWI GCNNNNNNNGC 2 cut(s) 38, 1526
BstNI CCWGG 1 cut(s) 1517
BstSCI CCNGG 1 cut(s) 1515
BstSFI CTRYAG 1 cut(s) 795
BstV1I GCAGC 1 cut(s) 25
BstX2I RGATCY 1 cut(s) 154
BstXI CCANNNNNNTGG 1 cut(s) 653
BstYI RGATCY 1 cut(s) 154
BsuRI GGCC 2 cut(s) 1026, 1730
BtsCI GGATG 3 cut(s) 453, 633, 1200
BtsIMutI CAGTG 2 cut(s) 1431, 1873
Cac8I GCNNGC 4 cut(s) 643, 712, 1028, 1525
CaiI CAGNNNCTG 1 cut(s) 541
CfoI GCGC 1 cut(s) 1529
CseI GACGC 1 cut(s) 551
Csp6I GTAC 6 cut(s) 631, 650, 978, 1058, 1721, 1835
CviAII CATG 9 cut(s) 132, 647, 722, 752, 760, 1309, 1345, 1700, 1726
CviQI GTAC 6 cut(s) 631, 650, 978, 1058, 1721, 1835
DdeI CTNAG 6 cut(s) 581, 947, 1355, 1373, 1622, 1794
DpnI GATC 6 cut(s) 156, 247, 1406, 1476, 1614, 1842
DpnII GATC 6 cut(s) 154, 245, 1404, 1474, 1612, 1840
EaeI YGGCCR 1 cut(s) 1728
Eam1104I CTCTTC 4 cut(s) 1116, 1143, 1490, 1555
EarI CTCTTC 4 cut(s) 1116, 1143, 1490, 1555
Eco130I CCWWGG 1 cut(s) 1731
Eco31I GGTCTC 2 cut(s) 1756, 1784
Eco57I CTGAAG 2 cut(s) 372, 1571
Eco88I CYCGRG 1 cut(s) 52
EcoRII CCWGG 1 cut(s) 1515
EcoT14I CCWWGG 1 cut(s) 1731
ErhI CCWWGG 1 cut(s) 1731
Esp3I CGTCTC 2 cut(s) 1093, 1602
FaeI CATG 9 cut(s) 135, 650, 725, 755, 763, 1312, 1348, 1703, 1729
FalI AAGNNNNNCTT 2 cut(s) 72, 104
FaqI GGGAC 1 cut(s) 948
FatI CATG 9 cut(s) 131, 646, 721, 751, 759, 1308, 1344, 1699, 1725
FauI CCCGC 1 cut(s) 1516
Fnu4HI GCNGC 2 cut(s) 39, 1521
FokI GGATG 3 cut(s) 440, 620, 1207
Fsp4HI GCNGC 2 cut(s) 39, 1521
FspBI CTAG 2 cut(s) 489, 1548
GlaI GCGC 1 cut(s) 1528
GluI GCNGC 2 cut(s) 39, 1521
HaeII RGCGCY 1 cut(s) 1530
HaeIII GGCC 2 cut(s) 1026, 1730
HapII CCGG 2 cut(s) 109, 824
HgaI GACGC 1 cut(s) 551
HhaI GCGC 1 cut(s) 1529
Hin1II CATG 9 cut(s) 135, 650, 725, 755, 763, 1312, 1348, 1703, 1729
Hin6I GCGC 1 cut(s) 1527
HinP1I GCGC 1 cut(s) 1527
HindIII AAGCTT 2 cut(s) 1370, 1392
HinfI GANTC 8 cut(s) 4, 112, 264, 511, 855, 866, 1288, 1648
HpaII CCGG 2 cut(s) 109, 824
HphI GGTGA 2 cut(s) 244, 380
Hpy166II GTNNAC 1 cut(s) 1850
Hpy188I TCNGA 9 cut(s) 245, 948, 1011, 1093, 1120, 1238, 1383, 1590, 1623
Hpy188III TCNNGA 7 cut(s) 293, 489, 768, 863, 1213, 1436, 1796
Hpy8I GTNNAC 1 cut(s) 1850
Hpy99I CGWCG 1 cut(s) 1848
HpyAV CCTTC 7 cut(s) 182, 1122, 1454, 1490, 1679, 1706, 1801
HpyCH4IV ACGT 1 cut(s) 1833
HpyF10VI GCNNNNNNNGC 2 cut(s) 38, 1526
HpyF3I CTNAG 6 cut(s) 581, 947, 1355, 1373, 1622, 1794
HpySE526I ACGT 1 cut(s) 1833
Hsp92II CATG 9 cut(s) 135, 650, 725, 755, 763, 1312, 1348, 1703, 1729
HspAI GCGC 1 cut(s) 1527
Kzo9I GATC 6 cut(s) 154, 245, 1404, 1474, 1612, 1840
LmnI GCTCC 2 cut(s) 17, 656
Lsp1109I GCAGC 1 cut(s) 25
LweI GCATC 5 cut(s) 712, 899, 1065, 1264, 1646
MaeI CTAG 2 cut(s) 489, 1548
MaeII ACGT 1 cut(s) 1833
MaeIII GTNAC 1 cut(s) 404
MalI GATC 6 cut(s) 156, 247, 1406, 1476, 1614, 1842
MbiI CCGCTC 1 cut(s) 375
MboI GATC 6 cut(s) 154, 245, 1404, 1474, 1612, 1840
MflI RGATCY 1 cut(s) 154
MlsI TGGCCA 1 cut(s) 1730
MluNI TGGCCA 1 cut(s) 1730
MlyI GAGTC 1 cut(s) 106
MmeI TCCRAC 1 cut(s) 1239
MnlI CCTC 5 cut(s) 216, 359, 540, 1087, 1556
Mox20I TGGCCA 1 cut(s) 1730
MroXI GAANNNNTTC 3 cut(s) 914, 1136, 1292
MscI TGGCCA 1 cut(s) 1730
MseI TTAA 7 cut(s) 327, 428, 606, 873, 1329, 1502, 1814
MslI CAYNNNNRTG 2 cut(s) 206, 1349
Msp20I TGGCCA 1 cut(s) 1730
MspCI CTTAAG 1 cut(s) 872
MspI CCGG 2 cut(s) 109, 824
MspR9I CCNGG 1 cut(s) 1517
Mva1269I GAATGC 2 cut(s) 564, 1479
MvaI CCWGG 1 cut(s) 1517
MwoI GCNNNNNNNGC 2 cut(s) 38, 1526
NdeII GATC 6 cut(s) 154, 245, 1404, 1474, 1612, 1840
NlaIII CATG 9 cut(s) 135, 650, 725, 755, 763, 1312, 1348, 1703, 1729
NlaIV GGNNCC 3 cut(s) 156, 658, 1365
NmeAIII GCCGAG 1 cut(s) 570
NspV TTCGAA 2 cut(s) 940, 1140
PcsI WCGNNNNNNNCGW 1 cut(s) 1843
PctI GAATGC 2 cut(s) 564, 1479
PdmI GAANNNNTTC 3 cut(s) 914, 1136, 1292
PfeI GAWTC 7 cut(s) 4, 264, 511, 855, 866, 1288, 1648
Pfl23II CGTACG 1 cut(s) 1834
PflMI CCANNNNNTGG 1 cut(s) 593
PkrI GCNGC 2 cut(s) 40, 1522
PleI GAGTC 1 cut(s) 106
PpsI GAGTC 1 cut(s) 106
PshBI ATTAAT 1 cut(s) 1814
Psp6I CCWGG 1 cut(s) 1515
PspGI CCWGG 1 cut(s) 1515
PspLI CGTACG 1 cut(s) 1834
PspN4I GGNNCC 3 cut(s) 156, 658, 1365
PstNI CAGNNNCTG 1 cut(s) 541
PsuI RGATCY 1 cut(s) 154
RsaI GTAC 6 cut(s) 632, 651, 979, 1059, 1722, 1836
RsaNI GTAC 6 cut(s) 631, 650, 978, 1058, 1721, 1835
RseI CAYNNNNRTG 2 cut(s) 206, 1349
SaqAI TTAA 7 cut(s) 327, 428, 606, 873, 1329, 1502, 1814
SatI GCNGC 2 cut(s) 39, 1521
Sau3AI GATC 6 cut(s) 154, 245, 1404, 1474, 1612, 1840
ScaI AGTACT 1 cut(s) 979
SchI GAGTC 1 cut(s) 106
ScrFI CCNGG 1 cut(s) 1517
SfaNI GCATC 5 cut(s) 712, 899, 1065, 1264, 1646
SfcI CTRYAG 1 cut(s) 795
SfuI TTCGAA 2 cut(s) 940, 1140
SmiMI CAYNNNNRTG 2 cut(s) 206, 1349
SmlI CTYRAG 1 cut(s) 872
SmoI CTYRAG 1 cut(s) 872
SsiI CCGC 3 cut(s) 375, 1520, 1523
SspI AATATT 3 cut(s) 219, 277, 806
SspMI CTAG 2 cut(s) 489, 1548
StyD4I CCNGG 1 cut(s) 1515
StyI CCWWGG 1 cut(s) 1731
TaiI ACGT 1 cut(s) 1836
TaqI TCGA 7 cut(s) 364, 432, 556, 940, 1073, 1140, 1318
TatI WGTACW 5 cut(s) 630, 649, 977, 1057, 1720
TauI GCSGC 1 cut(s) 1523
TfiI GAWTC 7 cut(s) 4, 264, 511, 855, 866, 1288, 1648
Tru1I TTAA 7 cut(s) 327, 428, 606, 873, 1329, 1502, 1814
Tru9I TTAA 7 cut(s) 327, 428, 606, 873, 1329, 1502, 1814
TscAI CASTG 1 cut(s) 1431
TseI GCWGC 1 cut(s) 38
TspGWI ACGGA 2 cut(s) 4, 1620
TspRI CASTG 1 cut(s) 1431
Van91I CCANNNNNTGG 1 cut(s) 593
Vha464I CTTAAG 1 cut(s) 872
VspI ATTAAT 1 cut(s) 1814
XapI RAATTY 4 cut(s) 288, 964, 1109, 1136
XbaI TCTAGA 1 cut(s) 488
XmnI GAANNNNTTC 3 cut(s) 914, 1136, 1292
XspI CTAG 2 cut(s) 489, 1548
ZrmI AGTACT 1 cut(s) 979
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.