pycom01g23090

Cyclic nucleotide-gated ion channel 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0000119
Physical Location & Seq
Reverse (-)
20530677 .. 20533847
3171 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g23090.7

Sequence Viewer

Length: 1884 bp
ATGCGCCATGACCGGCCCACAGAAGATACTTACGTAGGCTTGGTTGGGGGCTTCGCCTTCGGCTGTTCGGCAGTAGAGGCCGGAGAACGTTCGAAATCAACATGCAGCAGAGGGCTTAACTTGATAAAGAAGATTCGTAATGCGCAGCAGGGGCGTTACCTTCCAATATGGAGCAGAATATTTGTGCTATCATGCGTATTTGCTGTCTCTCTTGATCCTTTCTTCTTTTACGTTGTGGTCATCGATCAGGATAACAAGTGTTTTCAAATAGACAAAACGTTGGCTATTGTTGCTCTTCTTATGCGGTCGATCACGGATGTCATTTTCATGGTGCATTTAATATGTGAAATCTGTGACGGTGTTCAAAATCCAAATCCAAAAACCCTGAAAAAAGGAGCCCCTTCGACAGAGGAACAAACCGGGGGAAATTCAGATAAGAAGACGAAAATCAGAGAGTTGGCTGATGAAGTTGCCATGAAATTAGCTCAGAAGATGAGTAATTTTTACACAATGAATGGCTCAGGATTTGTGGAACACAAAAACATTCTGAACTTTTTTCTTCTCGGTCAATATTTGCCGAGGATTTATCGAATCAACCTATCGGCCAAGGAATTCAAAATGACTAGTGGGATATGGATTAAGGGTCTATACAACATGTTTCTCTACATTCTTTCCAGCCACGTACTCGGAGCCTTTTGGTACTTTTTTTCTGTTCAACGAGAGTTAACTTGTTGGCAAGAGGCTTGTGTAAATCACAGTAAAGATCCCAGGGCATGTTTGAATACTTTCAACTGCGATAGTCGAAGTACTACTGCAAGAAATATAACATTTCTAAATGAGCATTGCCCTTTGGATACTCCAAATGGTGCTTCATCTCCATTTAACTTTGGCATATTTCTTGATTCCCTTCAGAATCAAAACACGGAGCAAATACTGTTCAGAAAGAAGTTCTTTTACTCCTTCTGGTGGGGGTTGCGAAATCTAAGTAATTTTGGGACTAATCTGATGACGAGTACATATGTGTTCGAAAACTTGTTTGCGGTTCTCATTTCTGTAATTGGCTTGCTACTGTTTTTATATCTCATTGGAAACGTGCAGGTTGGTATATTCAATCTCTCTGTAGTAATTGTCTGTTTTGTGGCGAGACAGACTTTTATGCAGATGGAAGCTACAAAAACAGAAGAGCTGAGAGAAAAGATTAAGTTGAAGAAGCTAGACGTAAGGACATGGATGTCCAGAAATGAAATCCCTGATGATATGAAGGAAGAAATCTTGAGAAGCATAAAGCGAAAATTGAAACGACACATTGATGCTGATCTCCATCATTTTCTGTTCTATATTCTTCCTGTGAATATCAGAACATCTCTGAAACATTTTCTTTGCATTAAGACACTTCAGAAAGTAAAAAAGCTTGAAGATATGGATGTCAAAGTGTTGACGTTGATCTGCAACTATCTGAAGCCAGTGAGGTACACTGAAAACAGCTTCGTTTTTCGAATGGGAGATCCACTCGACTGCATGCTGTTCATTATCGAAGGGACAGTGTGGACCTACGGGTCGACTGAGAGTCAAGCTGGGCAAGGAATCTCATCAATGGACACCAAGCCCCTCGGGAAAGGTGACTATTACGGGGAAGAGCTTCTCGATTGCGCATCACACCATTTCACCAAACTTCCAGTCTCCAGCAAACATGTCAAAAGTCAGACAAAAGTAGAAGCATTTGTGCTCATGGCCAAGGACTTGGAAACTGTAGTTTCCAAATACCGGGTAAAGTGGGAGAAAAACGAGAAGAGGGGTTCTCAATTGTTGGAGTCCGTGGCAGCTTCTACCATAGTATCAGCATTCCGTCTTCATCTCCGCAAAAGGGAACGTGCACAACAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
Ion_trans

Protein Analysis

628

Amino Acids

72.07

Weight (kDa)

9.2

Isoelectric Point (pI)

36.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000119)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g10570 FvH4_4g36390 FvH4_7g31840 FvH4_7g31840 FvH4_7g31850 FvH4_7g31850 FvH4_7g31861 FvH4_7g31861 FvH4_7g31950
malus_domestica MD01G1054600.v1.1 MD01G1221100.v1.1 MD01G1221400.v1.1 MD01G1221600.v1.1 MD01G1221800.v1.1 MD01G1222000.v1.1 MD01G1222100.v1.1 MD07G1140800.v1.1 MD07G1291200.v1.1 MD07G1291400.v1.1 MD07G1291500.v1.1 MD07G1291700.v1.1 MD07G1292500.v1.1 MD07G1292700.v1.1 MD07G1292800.v1.1 MD07G1292900.v1.1 MD08G1241600.v1.1 MD11G1083800.v1.1 MD15G1431600.v1.1
prunus_persica Prupe.1G017800_v2.0.a1 Prupe.1G017800_v2.0.a1 Prupe.1G162800_v2.0.a1 Prupe.2G312500_v2.0.a1 Prupe.2G312500_v2.0.a1 Prupe.2G312700_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092600_v2.0.a1 Prupe.4G092800_v2.0.a1 Prupe.4G092800_v2.0.a1 Prupe.6G142800_v2.0.a1 Prupe.6G142800_v2.0.a1 Prupe.6G143000_v2.0.a1 Prupe.6G143100_v2.0.a1 Prupe.6G143100_v2.0.a1 Prupe.6G143100_v2.0.a1 Prupe.6G143100_v2.0.a1
pyrus_communis pycom01g23050 pycom01g23070 pycom01g23090 pycom01g23100 pycom01g23110 pycom01g23120 pycom01g23170 pycom07g26410 pycom07g26440 pycom07g26470 pycom07g26480 pycom07g26490 pycom07g26500 pycom07g26510 pycom07g26530 pycom07g26540 pycom07g26570 pycom07g26580 pycom07g26590 pycom08g20940
rosa_chinensis RchiOBHm_Chr1g0324571 RchiOBHm_Chr1g0336181 RchiOBHm_Chr1g0381061 RchiOBHm_Chr1g0381091 RchiOBHm_Chr1g0381121 RchiOBHm_Chr1g0381131 RchiOBHm_Chr1g0381141 RchiOBHm_Chr1g0381161 RchiOBHm_Chr1g0381211 RchiOBHm_Chr2g0110361 RchiOBHm_Chr2g0120231 RchiOBHm_Chr3g0486281 RchiOBHm_Chr3g0486311 RchiOBHm_Chr3g0486401 RchiOBHm_Chr4g0409511 RchiOBHm_Chr4g0409521 RchiOBHm_Chr5g0017271 RchiOBHm_Chr5g0048781 RchiOBHm_Chr5g0048811
rosa_laevigata RLG00000002401 RLG00000008559 RLG00000017798 RLG00000017799 RLG00000023082 RLG00000023088 RLG00000026230 RLG00000026238 RLG00000026240 RLG00000026241 RLG00000026242 RLG00000026243 RLG00000026244 RLG00000026246 RLG00000026247 RLG00000026248 RLG00000029876 RLG00000030189 RLG00000034579
rosa_multiflora Rmu_co8101374.1_g000001 Rmu_co8309149.1_g000001 Rmu_co8450063.1_g000001 Rmu_sc0000441.1_g000094 Rmu_sc0000479.1_g000005 Rmu_sc0000647.1_g000006 Rmu_sc0001393.1_g000031 Rmu_sc0001423.1_g000037 Rmu_sc0001616.1_g000015 Rmu_sc0001857.1_g000011 Rmu_sc0002183.1_g000013 Rmu_sc0002183.1_g000028 Rmu_sc0002183.1_g000030 Rmu_sc0002688.1_g000003 Rmu_sc0002688.1_g000020 Rmu_sc0003801.1_g000003 Rmu_sc0003801.1_g000004 Rmu_sc0004125.1_g000006 Rmu_sc0004494.1_g000005 Rmu_sc0004494.1_g000006 Rmu_sc0004494.1_g000008 Rmu_sc0004494.1_g000009 Rmu_sc0005842.1_g000004 Rmu_sc0006872.1_g000001 Rmu_sc0006872.1_g000005 Rmu_sc0006872.1_g000009 Rmu_sc0006872.1_g000010 Rmu_sc0008053.1_g000026
rosa_roxburghii Rroxscaffold_159G00432960 Rroxscaffold_1G00033060 Rroxscaffold_2G00123150 Rroxscaffold_2G00123170 Rroxscaffold_2G00133500 Rroxscaffold_2G00133550 Rroxscaffold_4G00278650 Rroxscaffold_4G00278750 Rroxscaffold_4G00278790 Rroxscaffold_4G00278800 Rroxscaffold_4G00278810 Rroxscaffold_4G00325390 Rroxscaffold_5G00354010 Rroxscaffold_6G00395890 Rroxscaffold_6G00395900 Rroxscaffold_6G00395920
rosa_rugosa Rorug01G0046900.1 Rorug01G0427000 Rorug01G0427100 Rorug01G0427200 Rorug01G0427300 Rorug01G0427400 Rorug01G0427500 Rorug01G0427500 Rorug01G0427600 Rorug01G0427600 Rorug01G0427700 Rorug01G0427700 Rorug01G0427800 Rorug01G0428700 Rorug02G0166400 Rorug02G0166500 Rorug02G0166500 Rorug02G0166600 Rorug02G0166600 Rorug02G0166700 Rorug02G0167300 Rorug02G0167300 Rorug02G0167300 Rorug03G0224600 Rorug03G0224600 Rorug04G0094400 Rorug05G0241100 Rorug05G0241200
rosa_samantha Rh1BG407700 Rh1BG407900 Rh1BG409000 Rh1CG064700 Rh1CG420900 Rh1CG421000 Rh1CG421200 Rh1CG421300 Rh1CG421800 Rh1CG421900 Rh1CG422000 Rh1CG422200 Rh1CG422300 Rh1CG423600 Rh1DG437600 Rh1DG438900 Rh1DG439000 Rh1DG440300 Rh2AG219100 Rh2CG220700 Rh2DG225300 Rh3AG274400 Rh3AG274600 Rh3CG308900 Rh3DG305800 Rh4AG156400 Rh5AG129000 Rh5AG320900 Rh5CG139600
rosa_wichuraiana Rw1G005300 Rw1G039280 Rw1G039300 Rw1G039310 Rw1G039320 Rw1G039330 Rw1G039340 Rw1G039360 Rw1G039390 Rw2G016910 Rw2G022800 Rw3G024330 Rw3G024340 Rw3G024350 Rw3G024400 Rw4G012810 Rw5G030120 Rw5G030280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 1231, 1555
Acc16I TGCGCA 2 cut(s) 144, 1651
Acc36I ACCTGC 1 cut(s) 1087
AccI GTMKAC 1 cut(s) 1559
AciI CCGC 3 cut(s) 304, 1040, 1858
AclI AACGTT 2 cut(s) 88, 278
AclWI GGATC 3 cut(s) 209, 758, 1499
AcoI YGGCCR 2 cut(s) 603, 1731
AcsI RAATTY 2 cut(s) 427, 611
AcuI CTGAAG 3 cut(s) 893, 1379, 1478
AfaI GTAC 5 cut(s) 684, 701, 808, 1015, 1472
AfiI CCNNNNNNNGG 3 cut(s) 966, 1764, 1864
AflIII ACRYGT 2 cut(s) 654, 1690
AhdI GACNNNNNGTC 1 cut(s) 1566
AhlI ACTAGT 1 cut(s) 623
AjnI CCWGG 1 cut(s) 767
AjuI GAANNNNNNNTTGG 2 cut(s) 853, 885
AluBI AGCT 9 cut(s) 485, 1169, 1186, 1213, 1411, 1485, 1574, 1639, 1823
AluI AGCT 9 cut(s) 485, 1169, 1186, 1213, 1411, 1485, 1574, 1639, 1823
Alw21I GWGCWC 2 cut(s) 1728, 1876
Alw26I GTCTC 3 cut(s) 211, 1138, 1684
Alw44I GTGCAC 1 cut(s) 1872
AlwI GGATC 3 cut(s) 209, 758, 1499
Ama87I CYCGRG 1 cut(s) 1610
AoxI GGCC 4 cut(s) 14, 78, 603, 1731
ApaLI GTGCAC 1 cut(s) 1872
ApeKI GCWGC 3 cut(s) 105, 145, 1820
ApoI RAATTY 2 cut(s) 427, 611
ArsI GACNNNNNNTTYG 2 cut(s) 1662, 1694
Asp700I GAANNNNTTC 2 cut(s) 785, 1638
AspLEI GCGC 3 cut(s) 6, 145, 1652
AspS9I GGNCC 2 cut(s) 15, 1548
AsuC2I CCSGG 2 cut(s) 421, 1766
AsuHPI GGTGA 2 cut(s) 1631, 1657
AsuII TTCGAA 3 cut(s) 92, 1026, 1495
AvaI CYCGRG 1 cut(s) 1610
AvaII GGWCC 1 cut(s) 1548
BaeGI GKGCMC 1 cut(s) 1876
BaeI ACNNNNGTAYC 4 cut(s) 18, 51, 1818, 1851
BalI TGGCCA 1 cut(s) 1733
BanII GRGCYC 1 cut(s) 400
BarI GAAGNNNNNNTAC 2 cut(s) 938, 970
BbsI GAAGAC 2 cut(s) 446, 1841
Bbv12I GWGCWC 2 cut(s) 1728, 1876
BbvI GCAGC 3 cut(s) 117, 157, 1832
BccI CCATC 2 cut(s) 1156, 1329
BciT130I CCWGG 1 cut(s) 769
BciVI GTATCC 1 cut(s) 847
BcnI CCSGG 2 cut(s) 421, 1766
BcoDI GTCTC 3 cut(s) 211, 1138, 1684
BcuI ACTAGT 1 cut(s) 623
BfaI CTAG 2 cut(s) 624, 1214
BfmI CTRYAG 2 cut(s) 1119, 1749
BfuAI ACCTGC 1 cut(s) 1087
BfuI GTATCC 1 cut(s) 847
BisI GCNGC 3 cut(s) 106, 146, 1821
BlsI GCNGC 3 cut(s) 107, 147, 1822
BmcAI AGTACT 1 cut(s) 808
Bme1390I CCNGG 3 cut(s) 421, 769, 1766
Bme18I GGWCC 1 cut(s) 1548
BmeRI GACNNNNNGTC 1 cut(s) 1566
BmeT110I CYCGRG 1 cut(s) 1610
BmgT120I GGNCC 2 cut(s) 15, 1548
BmiI GGNNCC 2 cut(s) 397, 691
BmrFI CCNGG 3 cut(s) 421, 769, 1766
BmsI GCATC 2 cut(s) 1300, 1661
BpiI GAAGAC 2 cut(s) 446, 1841
BplI GAGNNNNNCTC 4 cut(s) 1494, 1526, 1783, 1815
BpmI CTGGAG 1 cut(s) 1666
Bpu10I CCTNAGC 1 cut(s) 520
Bpu14I TTCGAA 3 cut(s) 92, 1026, 1495
BpuEI CTTGAG 1 cut(s) 1294
BpuMI CCSGG 2 cut(s) 421, 1766
Bsa29I ATCGAT 1 cut(s) 243
BsaAI YACGTR 2 cut(s) 34, 682
BsaBI GATNNNNATC 2 cut(s) 1314, 1320
BsaJI CCNNGG 8 cut(s) 420, 578, 606, 767, 768, 1609, 1734, 1815
Bsc4I CCNNNNNNNGG 3 cut(s) 966, 1764, 1864
Bse118I RCCGGY 1 cut(s) 12
Bse1I ACTGG 2 cut(s) 1463, 1676
Bse3DI GCAATG 1 cut(s) 841
Bse8I GATNNNNATC 2 cut(s) 1314, 1320
BseBI CCWGG 1 cut(s) 769
BseCI ATCGAT 1 cut(s) 243
BseDI CCNNGG 8 cut(s) 420, 578, 606, 767, 768, 1609, 1734, 1815
BseGI GGATG 3 cut(s) 322, 1236, 1429
BseJI GATNNNNATC 2 cut(s) 1314, 1320
BseLI CCNNNNNNNGG 3 cut(s) 966, 1764, 1864
BseMI GCAATG 1 cut(s) 841
BseMII CTCAG 4 cut(s) 500, 534, 1178, 1554
BseNI ACTGG 2 cut(s) 1463, 1676
BseSI GKGCMC 1 cut(s) 1876
BseXI GCAGC 3 cut(s) 117, 157, 1832
BseYI CCCAGC 1 cut(s) 1574
BsgI GTGCAG 1 cut(s) 1115
Bsh1285I CGRYCG 1 cut(s) 308
BshFI GGCC 4 cut(s) 16, 80, 605, 1733
BshVI ATCGAT 1 cut(s) 243
BsiEI CGRYCG 1 cut(s) 308
BsiHKAI GWGCWC 2 cut(s) 1728, 1876
BsiHKCI CYCGRG 1 cut(s) 1610
BsiSI CCGG 4 cut(s) 13, 81, 420, 1765
BslFI GGGAC 2 cut(s) 1009, 1552
BslI CCNNNNNNNGG 3 cut(s) 966, 1764, 1864
BsmAI GTCTC 3 cut(s) 211, 1138, 1684
BsmFI GGGAC 2 cut(s) 1009, 1552
BsmI GAATGC 1 cut(s) 1841
BsnI GGCC 4 cut(s) 16, 80, 605, 1733
BsoBI CYCGRG 1 cut(s) 1610
Bsp119I TTCGAA 3 cut(s) 92, 1026, 1495
Bsp1286I GDGCHC 3 cut(s) 400, 1728, 1876
Bsp143I GATC 7 cut(s) 214, 244, 309, 763, 1315, 1443, 1504
BspACI CCGC 3 cut(s) 304, 1040, 1858
BspANI GGCC 4 cut(s) 16, 80, 605, 1733
BspCNI CTCAG 4 cut(s) 499, 533, 1179, 1555
BspDI ATCGAT 1 cut(s) 243
BspLI GGNNCC 2 cut(s) 397, 691
BspMI ACCTGC 1 cut(s) 1087
BspPI GGATC 3 cut(s) 209, 758, 1499
BspQI GCTCTTC 3 cut(s) 300, 1176, 1629
BspT104I TTCGAA 3 cut(s) 92, 1026, 1495
BsrDI GCAATG 1 cut(s) 841
BsrFI RCCGGY 1 cut(s) 12
BsrI ACTGG 2 cut(s) 1463, 1676
BssAI RCCGGY 1 cut(s) 12
BssECI CCNNGG 8 cut(s) 420, 578, 606, 767, 768, 1609, 1734, 1815
BssMI GATC 7 cut(s) 214, 244, 309, 763, 1315, 1443, 1504
BssT1I CCWWGG 2 cut(s) 606, 1734
Bst2UI CCWGG 1 cut(s) 769
Bst4CI ACNGT 6 cut(s) 359, 758, 936, 1071, 1543, 1750
Bst6I CTCTTC 4 cut(s) 300, 1176, 1629, 1784
BstBAI YACGTR 2 cut(s) 34, 682
BstBI TTCGAA 3 cut(s) 92, 1026, 1495
BstC8I GCNNGC 2 cut(s) 1064, 1520
BstDEI CTNAG 5 cut(s) 486, 520, 983, 1187, 1563
BstDSI CCRYGG 1 cut(s) 1815
BstF5I GGATG 3 cut(s) 322, 1236, 1429
BstHHI GCGC 3 cut(s) 6, 145, 1652
BstKTI GATC 7 cut(s) 217, 247, 312, 766, 1318, 1446, 1507
BstMAI GTCTC 3 cut(s) 211, 1138, 1684
BstMBI GATC 7 cut(s) 214, 244, 309, 763, 1315, 1443, 1504
BstMCI CGRYCG 1 cut(s) 308
BstMWI GCNNNNNNNGC 3 cut(s) 77, 151, 290
BstNI CCWGG 1 cut(s) 769
BstNSI RCATGY 5 cut(s) 105, 658, 777, 1522, 1694
BstSCI CCNGG 3 cut(s) 419, 767, 1764
BstSFI CTRYAG 2 cut(s) 1119, 1749
BstSLI GKGCMC 1 cut(s) 1876
BstSNI TACGTA 1 cut(s) 34
BstV1I GCAGC 3 cut(s) 117, 157, 1832
BstV2I GAAGAC 2 cut(s) 446, 1841
BstX2I RGATCY 2 cut(s) 763, 1504
BstXI CCANNNNNNTGG 1 cut(s) 1741
BstYI RGATCY 2 cut(s) 763, 1504
Bsu15I ATCGAT 1 cut(s) 243
BsuI GTATCC 1 cut(s) 847
BsuRI GGCC 4 cut(s) 16, 80, 605, 1733
BsuTUI ATCGAT 1 cut(s) 243
BtgI CCRYGG 1 cut(s) 1815
BtsCI GGATG 3 cut(s) 322, 1236, 1429
BtsIMutI CAGTG 3 cut(s) 1470, 1473, 1548
BveI ACCTGC 1 cut(s) 1087
Cac8I GCNNGC 2 cut(s) 1064, 1520
CfoI GCGC 3 cut(s) 6, 145, 1652
Cfr10I RCCGGY 1 cut(s) 12
Cfr13I GGNCC 2 cut(s) 15, 1548
ClaI ATCGAT 1 cut(s) 243
Csp6I GTAC 5 cut(s) 683, 700, 807, 1014, 1471
CviQI GTAC 5 cut(s) 683, 700, 807, 1014, 1471
DdeI CTNAG 5 cut(s) 486, 520, 983, 1187, 1563
DpnI GATC 7 cut(s) 216, 246, 311, 765, 1317, 1445, 1506
DpnII GATC 7 cut(s) 214, 244, 309, 763, 1315, 1443, 1504
DrdI GACNNNNNNGTC 2 cut(s) 1231, 1555
DriI GACNNNNNGTC 1 cut(s) 1566
DseDI GACNNNNNNGTC 2 cut(s) 1231, 1555
EaeI YGGCCR 2 cut(s) 603, 1731
Eam1104I CTCTTC 4 cut(s) 300, 1176, 1629, 1784
Eam1105I GACNNNNNGTC 1 cut(s) 1566
EarI CTCTTC 4 cut(s) 300, 1176, 1629, 1784
Eco105I TACGTA 1 cut(s) 34
Eco130I CCWWGG 2 cut(s) 606, 1734
Eco24I GRGCYC 1 cut(s) 400
Eco47I GGWCC 1 cut(s) 1548
Eco57I CTGAAG 3 cut(s) 893, 1379, 1478
Eco88I CYCGRG 1 cut(s) 1610
EcoRI GAATTC 1 cut(s) 611
EcoRII CCWGG 1 cut(s) 767
EcoT14I CCWWGG 2 cut(s) 606, 1734
EcoT38I GRGCYC 1 cut(s) 400
ErhI CCWWGG 2 cut(s) 606, 1734
FalI AAGNNNNNCTT 2 cut(s) 935, 967
FaqI GGGAC 2 cut(s) 1009, 1552
FauNDI CATATG 1 cut(s) 1018
FblI GTMKAC 1 cut(s) 1559
Fnu4HI GCNGC 3 cut(s) 106, 146, 1821
FokI GGATG 3 cut(s) 329, 1243, 1436
FriOI GRGCYC 1 cut(s) 400
Fsp4HI GCNGC 3 cut(s) 106, 146, 1821
FspBI CTAG 2 cut(s) 624, 1214
FspI TGCGCA 2 cut(s) 144, 1651
GlaI GCGC 3 cut(s) 5, 144, 1651
GluI GCNGC 3 cut(s) 106, 146, 1821
GsaI CCCAGC 1 cut(s) 1578
GsuI CTGGAG 1 cut(s) 1666
HaeIII GGCC 4 cut(s) 16, 80, 605, 1733
HapII CCGG 4 cut(s) 13, 81, 420, 1765
HhaI GCGC 3 cut(s) 6, 145, 1652
Hin6I GCGC 3 cut(s) 4, 143, 1650
HinP1I GCGC 3 cut(s) 4, 143, 1650
HincII GTYRAC 3 cut(s) 726, 1437, 1560
HindII GTYRAC 3 cut(s) 726, 1437, 1560
HindIII AAGCTT 1 cut(s) 1409
HinfI GANTC 7 cut(s) 133, 591, 902, 913, 1567, 1584, 1811
HpaI GTTAAC 1 cut(s) 726
HpaII CCGG 4 cut(s) 13, 81, 420, 1765
HphI GGTGA 2 cut(s) 1631, 1657
Hpy166II GTNNAC 6 cut(s) 726, 1437, 1473, 1548, 1560, 1874
Hpy188III TCNNGA 8 cut(s) 212, 248, 522, 899, 1236, 1273, 1612, 1643
Hpy8I GTNNAC 6 cut(s) 726, 1437, 1473, 1548, 1560, 1874
HpyAV CCTTC 7 cut(s) 67, 170, 411, 917, 970, 1255, 1529
HpyCH4III ACNGT 6 cut(s) 359, 758, 936, 1071, 1543, 1750
HpyCH4IV ACGT 9 cut(s) 33, 88, 231, 278, 681, 1092, 1218, 1439, 1870
HpyCH4V TGCA 9 cut(s) 105, 334, 815, 1096, 1159, 1383, 1449, 1518, 1874
HpyF10VI GCNNNNNNNGC 3 cut(s) 77, 151, 290
HpyF3I CTNAG 5 cut(s) 486, 520, 983, 1187, 1563
HpySE526I ACGT 9 cut(s) 33, 88, 231, 278, 681, 1092, 1218, 1439, 1870
HspAI GCGC 3 cut(s) 4, 143, 1650
KspAI GTTAAC 1 cut(s) 726
Kzo9I GATC 7 cut(s) 214, 244, 309, 763, 1315, 1443, 1504
LguI GCTCTTC 3 cut(s) 300, 1176, 1629
LmnI GCTCC 4 cut(s) 171, 395, 689, 925
Lsp1109I GCAGC 3 cut(s) 117, 157, 1832
LweI GCATC 2 cut(s) 1300, 1661
MaeI CTAG 2 cut(s) 624, 1214
MaeII ACGT 9 cut(s) 33, 88, 231, 278, 681, 1092, 1218, 1439, 1870
MaeIII GTNAC 3 cut(s) 155, 353, 1619
MalI GATC 7 cut(s) 216, 246, 311, 765, 1317, 1445, 1506
MboI GATC 7 cut(s) 214, 244, 309, 763, 1315, 1443, 1504
MfeI CAATTG 1 cut(s) 1802
MflI RGATCY 2 cut(s) 763, 1504
MhlI GDGCHC 3 cut(s) 400, 1728, 1876
MlsI TGGCCA 1 cut(s) 1733
MluCI AATT 9 cut(s) 427, 479, 499, 611, 988, 1056, 1125, 1292, 1802
MluNI TGGCCA 1 cut(s) 1733
MlyI GAGTC 2 cut(s) 1576, 1820
MmeI TCCRAC 1 cut(s) 1788
MnlI CCTC 8 cut(s) 70, 104, 403, 573, 733, 1461, 1619, 1785
Mox20I TGGCCA 1 cut(s) 1733
MroXI GAANNNNTTC 2 cut(s) 785, 1638
MscI TGGCCA 1 cut(s) 1733
MseI TTAA 7 cut(s) 117, 338, 639, 725, 882, 1200, 1386
MslI CAYNNNNRTG 2 cut(s) 326, 1308
Msp20I TGGCCA 1 cut(s) 1733
MspI CCGG 4 cut(s) 13, 81, 420, 1765
MspR9I CCNGG 3 cut(s) 421, 769, 1766
MunI CAATTG 1 cut(s) 1802
Mva1269I GAATGC 1 cut(s) 1841
MvaI CCWGG 1 cut(s) 769
MwoI GCNNNNNNNGC 3 cut(s) 77, 151, 290
NciI CCSGG 2 cut(s) 421, 1766
NdeI CATATG 1 cut(s) 1018
NdeII GATC 7 cut(s) 214, 244, 309, 763, 1315, 1443, 1504
NlaIV GGNNCC 2 cut(s) 397, 691
NmeAIII GCCGAG 1 cut(s) 603
NmuCI GTSAC 2 cut(s) 353, 1619
NsbI TGCGCA 2 cut(s) 144, 1651
NspI RCATGY 5 cut(s) 105, 658, 777, 1522, 1694
NspV TTCGAA 3 cut(s) 92, 1026, 1495
PaeI GCATGC 1 cut(s) 1522
PasI CCCWGGG 1 cut(s) 768
PciI ACATGT 2 cut(s) 654, 1690
PciSI GCTCTTC 3 cut(s) 300, 1176, 1629
PctI GAATGC 1 cut(s) 1841
PdmI GAANNNNTTC 2 cut(s) 785, 1638
PfeI GAWTC 5 cut(s) 133, 591, 902, 913, 1584
PkrI GCNGC 3 cut(s) 107, 147, 1822
PleI GAGTC 2 cut(s) 1575, 1819
PpsI GAGTC 2 cut(s) 1575, 1819
Ppu21I YACGTR 2 cut(s) 34, 682
PscI ACATGT 2 cut(s) 654, 1690
Psp1406I AACGTT 2 cut(s) 88, 278
Psp6I CCWGG 1 cut(s) 767
PspFI CCCAGC 1 cut(s) 1574
PspGI CCWGG 1 cut(s) 767
PspN4I GGNNCC 2 cut(s) 397, 691
PspPI GGNCC 2 cut(s) 15, 1548
PsuI RGATCY 2 cut(s) 763, 1504
RsaI GTAC 5 cut(s) 684, 701, 808, 1015, 1472
RsaNI GTAC 5 cut(s) 683, 700, 807, 1014, 1471
RseI CAYNNNNRTG 2 cut(s) 326, 1308
SalI GTCGAC 1 cut(s) 1558
SapI GCTCTTC 3 cut(s) 300, 1176, 1629
SaqAI TTAA 7 cut(s) 117, 338, 639, 725, 882, 1200, 1386
SatI GCNGC 3 cut(s) 106, 146, 1821
Sau3AI GATC 7 cut(s) 214, 244, 309, 763, 1315, 1443, 1504
Sau96I GGNCC 2 cut(s) 15, 1548
ScaI AGTACT 1 cut(s) 808
SchI GAGTC 2 cut(s) 1576, 1820
ScrFI CCNGG 3 cut(s) 421, 769, 1766
SduI GDGCHC 3 cut(s) 400, 1728, 1876
SfaNI GCATC 2 cut(s) 1300, 1661
SfcI CTRYAG 2 cut(s) 1119, 1749
SfuI TTCGAA 3 cut(s) 92, 1026, 1495
SinI GGWCC 1 cut(s) 1548
SmiMI CAYNNNNRTG 2 cut(s) 326, 1308
SmlI CTYRAG 1 cut(s) 1273
SmoI CTYRAG 1 cut(s) 1273
SnaBI TACGTA 1 cut(s) 34
SpeI ACTAGT 1 cut(s) 623
SphI GCATGC 1 cut(s) 1522
Sse9I AATT 9 cut(s) 427, 479, 499, 611, 988, 1056, 1125, 1292, 1802
SsiI CCGC 3 cut(s) 304, 1040, 1858
SspI AATATT 2 cut(s) 180, 572
SspMI CTAG 2 cut(s) 624, 1214
StyD4I CCNGG 3 cut(s) 419, 767, 1764
StyI CCWWGG 2 cut(s) 606, 1734
TaaI ACNGT 6 cut(s) 359, 758, 936, 1071, 1543, 1750
TaiI ACGT 9 cut(s) 36, 91, 234, 281, 684, 1095, 1221, 1442, 1873
TaqII GACCGA 1 cut(s) 554
TasI AATT 9 cut(s) 427, 479, 499, 611, 988, 1056, 1125, 1292, 1802
TatI WGTACW 2 cut(s) 806, 1013
TfiI GAWTC 5 cut(s) 133, 591, 902, 913, 1584
Tru1I TTAA 7 cut(s) 117, 338, 639, 725, 882, 1200, 1386
Tru9I TTAA 7 cut(s) 117, 338, 639, 725, 882, 1200, 1386
TscAI CASTG 3 cut(s) 1470, 1480, 1548
TseFI GTSAC 2 cut(s) 353, 1619
TseI GCWGC 3 cut(s) 105, 145, 1820
Tsp45I GTSAC 2 cut(s) 353, 1619
TspDTI ATGAA 9 cut(s) 316, 480, 491, 527, 861, 1257, 1274, 1516, 1841
TspGWI ACGGA 4 cut(s) 329, 938, 1804, 1835
TspRI CASTG 3 cut(s) 1470, 1480, 1548
VneI GTGCAC 1 cut(s) 1872
VpaK11BI GGWCC 1 cut(s) 1548
XapI RAATTY 2 cut(s) 427, 611
XceI RCATGY 5 cut(s) 105, 658, 777, 1522, 1694
XmiI GTMKAC 1 cut(s) 1559
XmnI GAANNNNTTC 2 cut(s) 785, 1638
XspI CTAG 2 cut(s) 624, 1214
ZrmI AGTACT 1 cut(s) 808
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.